##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.4d.R.1.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24134818
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	76-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.46320502603334	36.0	34.0	38.0	31.0	39.0
2	34.79489420637023	35.0	34.0	38.0	31.0	39.0
3	34.949730799710196	35.0	34.0	38.0	31.0	39.0
4	34.902315153153424	36.0	34.0	38.0	31.0	39.0
5	34.76700632256684	35.0	34.0	37.0	31.0	39.0
6	34.75096418792137	35.0	34.0	37.0	31.0	39.0
7	34.74337685082191	35.0	34.0	37.0	32.0	39.0
8	34.897137405386694	35.0	34.0	37.0	32.0	39.0
9	34.884451625034	35.0	34.0	37.0	32.0	39.0
10-14	34.8600941345404	35.0	34.0	37.0	31.6	39.0
15-19	34.85593194031958	35.0	34.0	37.0	31.0	39.0
20-24	34.86494085018582	35.0	34.0	37.0	31.0	39.0
25-29	34.84864484994252	35.0	34.0	37.0	31.0	39.0
30-34	34.83816230145179	35.0	34.0	37.0	31.0	39.0
35-39	34.83361845115219	35.0	34.0	37.0	31.0	39.0
40-44	34.76887780135736	35.0	34.0	37.0	31.0	39.0
45-49	34.67566940840407	35.0	34.0	37.0	31.0	39.0
50-54	34.63753026851083	35.0	34.0	37.0	31.0	39.0
55-59	34.62282848787176	35.0	34.0	37.0	31.0	39.0
60-64	34.59767485298625	35.0	34.0	37.0	31.0	39.0
65-69	34.575850308877406	35.0	34.0	37.0	30.4	39.0
70-74	34.54819497706591	35.0	34.0	37.0	30.0	39.0
75-79	34.52326469406489	35.0	34.0	37.0	30.0	39.0
80-84	34.50036834578225	35.0	34.0	37.0	30.0	39.0
85-89	34.47608947476381	35.0	34.0	37.0	30.0	39.0
90-94	34.45060506365799	35.0	34.0	37.0	30.0	39.0
95-99	34.41671466876952	35.0	34.0	37.0	30.0	39.0
100-104	34.379082325191995	35.0	34.0	37.0	30.0	39.0
105-109	34.32505881521455	35.0	34.0	37.0	30.0	39.0
110-114	34.28751666689307	35.0	34.0	37.0	30.0	39.0
115-119	34.23670674515317	35.0	34.0	37.0	30.0	39.0
120-124	34.20292372416772	35.0	34.0	37.0	29.6	39.0
125-129	34.152630699097564	35.0	34.0	37.0	29.0	39.0
130-134	34.09642702943393	35.0	34.0	37.0	29.0	39.0
135-139	34.05099877634079	35.0	34.0	37.0	29.0	39.0
140-144	34.023570596737244	35.0	34.0	37.0	29.0	39.0
145-149	34.03458492586566	35.0	34.0	37.0	29.0	39.0
150	34.944831608686606	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	14.0
22	277.0
23	3227.0
24	21721.0
25	86735.0
26	215509.0
27	385937.0
28	563854.0
29	732805.0
30	918633.0
31	1154089.0
32	1478444.0
33	1957415.0
34	2720638.0
35	4255015.0
36	8597697.0
37	1042801.0
38	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.098980236768305	10.665147754584268	15.543775801416857	38.69209620723056
2	22.46165077949747	12.402313176254351	36.620172216195876	28.515863828052296
3	21.144034198248892	17.1545133390979	28.38435847187151	33.31709399078169
4	26.197395263702987	24.90652088991483	22.40425486778347	26.491828978598708
5	25.827330039445933	30.648234752746717	24.502508949108602	19.021926258698745
6	19.22381805032193	36.35976342050412	25.817337243868348	18.599081285305598
7	15.971295367100922	24.926914602080483	40.34632592466142	18.75546410615717
8	18.3671104270274	24.339917823219402	31.689861253448235	25.60311049630496
9	19.44240681505544	23.09268359381474	32.850924805674794	24.613984785455028
10-14	20.180562467202936	29.21487109111467	27.180411548092962	23.42415489358943
15-19	20.35672474067865	27.868041941952814	27.96521545666058	23.81001786070796
20-24	20.523362658238995	28.04687095694905	27.674623857069314	23.755142527742645
25-29	20.51211405191837	28.229889842483054	27.56183565498722	23.696160450611362
30-34	20.468772879468215	27.978738435186283	27.588891149356755	23.963597535988743
35-39	20.5769046928793	27.958764467420146	27.607836529878284	23.856494309822278
40-44	20.603735275174593	28.052577339638436	27.522795252747173	23.820892132439795
45-49	20.709252267395097	27.847083314984854	27.465410713301058	23.978253704318988
50-54	20.81131571163419	27.814263121311438	27.566801828717573	23.8076193383368
55-59	20.81464254314093	27.857193152143783	27.488624968993875	23.839539335721412
60-64	20.763451742661225	27.774328257085195	27.469289307858702	23.992930692394875
65-69	20.858745663429705	27.719339749445332	27.540591610579884	23.88132297654508
70-74	20.973277005870735	27.642864442948234	27.53462953852532	23.849229012655712
75-79	20.976328294829	27.550857073551043	27.54035686328995	23.93245776833001
80-84	21.030062049257932	27.55975387268287	27.497470180791673	23.912713897267526
85-89	21.004263176898622	27.568800007504557	27.57315225126521	23.853784564331615
90-94	21.00739210116797	27.464512150061704	27.511338646617705	24.016757102152614
95-99	21.08319567400458	27.3791147982467	27.627485399641976	23.910204128106745
100-104	21.14821493047995	27.431684137162776	27.488430381576457	23.93167055078082
105-109	21.093384248273313	27.26949740142378	27.62328082315801	24.013837527144897
110-114	21.203506137366297	27.241121990873346	27.645429095218145	23.909942776542216
115-119	21.23241057146433	27.277350843714466	27.60640637994094	23.883832204880264
120-124	21.178703896241743	27.21294409865497	27.595707510001176	24.012644495102116
125-129	21.21701088490705	27.12091607560452	27.67809912003225	23.98397391945618
130-134	21.294205425485917	27.225722826850262	27.572604199145957	23.907467548517864
135-139	21.28928227435703	27.076900816475614	27.66575653020405	23.96806037896331
140-144	21.338811283107948	27.011314870956436	27.78669526630854	23.86317857962707
145-149	20.960431161314087	27.16481527001876	27.882121246430113	23.992632322237036
150	20.932679856860005	25.776269504815154	28.550154944766533	24.74089569355831
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	3.0
9	4.5
10	5.5
11	5.5
12	7.5
13	15.5
14	35.5
15	75.0
16	164.5
17	348.5
18	671.5
19	1245.0
20	2324.0
21	3916.5
22	6146.0
23	9750.0
24	15169.0
25	23446.0
26	34746.5
27	48633.0
28	65770.0
29	91499.0
30	124349.0
31	158551.0
32	190371.0
33	225256.5
34	265122.0
35	313190.5
36	387207.5
37	487096.0
38	604998.0
39	751562.0
40	921033.5
41	1086004.5
42	1254837.5
43	1431619.5
44	1575035.0
45	1659025.5
46	1681352.0
47	1641953.5
48	1553882.0
49	1426158.5
50	1260057.5
51	1079371.5
52	897634.0
53	718560.0
54	555935.5
55	420023.5
56	315505.0
57	236005.0
58	176787.5
59	130625.5
60	91018.5
61	63536.5
62	45219.0
63	29944.0
64	19983.5
65	14855.5
66	13210.0
67	9640.0
68	4890.5
69	3074.5
70	2876.5
71	1998.5
72	866.5
73	301.5
74	138.5
75	91.5
76	50.0
77	30.5
78	11.5
79	1.5
80	1.5
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.5744887738536084E-4
3	6.215087265211613E-5
4	5.800748114197505E-5
5	4.9720698121692906E-5
6	5.3864089631833976E-5
7	1.5744887738536084E-4
8	8.28678302028215E-5
9	7.458104718253935E-5
10-14	1.4419002455290942E-4
15-19	2.1711371513139232E-4
20-24	1.7816583493606623E-4
25-29	2.8672269250176237E-4
30-34	7.789576039065221E-4
35-39	7.308942623888856E-4
40-44	0.0010499354086697483
45-49	8.551960076931179E-4
50-54	5.568718189629605E-4
55-59	2.925234406159599E-4
60-64	2.651770566490288E-4
65-69	3.1986982458289103E-4
70-74	3.7207655761066857E-4
75-79	2.461174944533031E-4
80-84	2.825795492092148E-4
85-89	3.795354297400946E-4
90-94	3.787075562988352E-4
95-99	3.23186688367907E-4
100-104	4.3257460824340526E-4
105-109	2.842411258739617E-4
110-114	2.510950291512628E-4
115-119	2.3949516011357772E-4
120-124	2.4115538389712023E-4
125-129	1.533145012297232E-4
130-134	1.533187805909297E-4
135-139	1.7487770826725E-4
140-144	1.5422132112749516E-4
145-149	1.4536955374657232E-4
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
76-77	6.0
78-79	6.0
80-81	9.0
82-83	10.0
84-85	7.0
86-87	19.0
88-89	23.0
90-91	18.0
92-93	24.0
94-95	26.0
96-97	24.0
98-99	34.0
100-101	40.0
102-103	59.0
104-105	44.0
106-107	56.0
108-109	55.0
110-111	67.0
112-113	72.0
114-115	73.0
116-117	88.0
118-119	109.0
120-121	126.0
122-123	146.0
124-125	172.0
126-127	215.0
128-129	217.0
130-131	313.0
132-133	437.0
134-135	590.0
136-137	943.0
138-139	2760.0
140-141	5964.0
142-143	9928.0
144-145	75553.0
146-147	770122.0
148-149	1415128.0
150-151	2.1851335E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.10230572642678
#Duplication Level	Percentage of total
1	38.571108843147144
2	10.52216863468386
3	6.43064089059411
4	4.645916835937349
5	3.395022897740713
6	2.733083678592687
7	2.2271563112072466
8	1.8821905443185627
9	1.5601813470934156
>10	18.22793668859715
>50	4.366858129216083
>100	4.667651902760998
>500	0.423476770920831
>1k	0.3239979607022941
>5k	0.02260856448764985
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	2.0716957550705376E-5	0.0	0.0	1.9473940097663051E-4	1.65735660405643E-5
2	4.143391510141075E-6	1.160149622839501E-4	0.0	0.0	0.001984684533357575	2.0716957550705376E-5
3	4.143391510141075E-6	1.2430174530423226E-4	0.0	0.0	0.003095113458075383	5.800748114197505E-5
4	2.9003740570987526E-5	1.4087531134479654E-4	0.0	4.143391510141075E-6	0.004508009963033489	7.043765567239827E-5
5	4.143391510141075E-5	1.4501870285493762E-4	0.0	4.143391510141075E-6	0.005780031156646799	7.043765567239827E-5
6	5.800748114197505E-5	1.4501870285493762E-4	0.0	4.143391510141075E-6	0.006907033647405172	8.28678302028215E-5
7	6.215087265211613E-5	1.9473940097663051E-4	0.0	4.143391510141075E-6	0.008083756836285238	8.28678302028215E-5
8	7.043765567239827E-5	1.988827924867716E-4	0.0	4.143391510141075E-6	0.010167882765886198	8.701122171296258E-5
9	8.28678302028215E-5	1.988827924867716E-4	0.0	4.143391510141075E-6	0.013747773030648086	9.94413962433858E-5
10-11	1.325885283245144E-4	2.092412712621243E-4	0.0	8.28678302028215E-6	0.018367654564455386	1.4709039861000818E-4
12-13	1.9681109673170107E-4	2.1959975003747696E-4	0.0	1.0358478775352688E-5	0.022260370888232926	1.988827924867716E-4
14-15	2.0716957550705376E-4	2.3410162032297074E-4	0.0	1.2430174530423225E-5	0.026621290452656408	2.2581483730268857E-4
16-17	2.4031670758818235E-4	2.548185778736761E-4	0.0	1.65735660405643E-5	0.030998783583120457	2.320299245679002E-4
18-19	2.6724875240409935E-4	2.734638396693109E-4	0.0	1.65735660405643E-5	0.034615964371473615	2.423884033432529E-4
20-21	3.335430165663565E-4	3.418297995866387E-4	0.0	1.65735660405643E-5	0.038218643289541276	2.651770566490288E-4
22-23	4.2262593403438964E-4	3.5633166987213247E-4	0.0	1.65735660405643E-5	0.04212793317935938	2.817506226895931E-4
24-25	5.117088515024228E-4	3.5633166987213247E-4	0.0	2.278865330577591E-5	0.04636247930272356	2.9003740570987524E-4
26-27	5.469276793386218E-4	3.5633166987213247E-4	0.0	2.9003740570987526E-5	0.05060531220910802	3.273279293011449E-4
28-29	5.717880283994684E-4	3.5633166987213247E-4	0.0	3.31471320811286E-5	0.054991092122592344	3.5011658260692086E-4
30-31	6.256521180313024E-4	3.5840336562720297E-4	0.0	4.7649002366622364E-5	0.05941209086391287	3.749769316677673E-4
32-33	6.650143373776425E-4	3.687618444025557E-4	0.0	5.593578538690452E-5	0.06330066379618027	4.184825425242486E-4
34-35	7.416670803152524E-4	3.7290523591269675E-4	0.0	7.66527429376099E-5	0.06667959957270032	4.288410212996013E-4
36-37	7.582406463558167E-4	3.811920189329789E-4	0.0	7.872443869268042E-5	0.06993216190816107	4.4955797885030664E-4
38-39	7.768859081514516E-4	3.915504977083316E-4	0.0	7.872443869268042E-5	0.07420607025087159	4.764900236662236E-4
40-41	8.328216935383562E-4	3.9362219346340214E-4	0.0	8.493952595789203E-5	0.07756843246135106	4.827051109314352E-4
42-43	8.390367808035677E-4	3.977655849735432E-4	0.0	9.94413962433858E-5	0.08098673045721745	4.8477680668650576E-4
44-45	8.618254341093436E-4	4.2676932554453075E-4	0.0	9.94413962433858E-5	0.08511147670556289	4.930635897067879E-4
46-47	9.053310449658249E-4	4.2676932554453075E-4	0.0	9.94413962433858E-5	0.08915335512370551	4.97206981216929E-4
48-49	9.36406481291883E-4	4.371278043198834E-4	0.0	9.94413962433858E-5	0.09304192805597292	5.137805472574933E-4
50-51	9.529800473324472E-4	4.474862830952361E-4	0.0	9.94413962433858E-5	0.09699058016513736	5.158522430125639E-4
52-53	0.0010151309199845633	4.474862830952361E-4	0.0	1.0151309199845634E-4	0.10118990746066533	5.179239387676344E-4
54-55	0.0010627799223511857	4.474862830952361E-4	0.0	1.0358478775352688E-4	0.10441553775131016	5.220673302777755E-4
56-57	0.001110428924717808	4.66131544890871E-4	0.0	1.0358478775352688E-4	0.1083704049477398	5.365692005632692E-4
58-59	0.001133217578023584	5.0135037272707E-4	0.0	1.0772817926366795E-4	0.11229834009935355	5.489993750936924E-4
60-61	0.0011808665803902064	5.179239387676344E-4	0.0	1.1187157077380902E-4	0.11576221540183149	5.655729411342568E-4
62-63	0.0012430174530423225	5.262107217879165E-4	0.0	1.1187157077380902E-4	0.11904170978210815	5.676446368893273E-4
64-65	0.0012616627148379573	5.303541132980576E-4	0.0	1.1601496228395009E-4	0.12338812747624614	5.717880283994684E-4
66-67	0.0012865230638988037	5.386408963183397E-4	0.0	1.2015835379409118E-4	0.1271669005334948	5.7800311566468E-4
68-69	0.0013631758068364138	5.448559835835513E-4	0.0	1.2223004954916173E-4	0.13017914616136736	5.966483774603148E-4
70-71	0.0014854058563855753	5.635012453791862E-4	0.0	1.2430174530423226E-4	0.1329117128623054	5.966483774603148E-4
72-73	0.0015413416417724799	5.635012453791862E-4	0.0	1.2430174530423226E-4	0.13572093230618104	6.007917689704559E-4
74-75	0.0016097076016898077	5.676446368893273E-4	0.0	1.2844513681437331E-4	0.1388678381581332	6.152936392559497E-4
76-77	0.0016697867785868533	5.676446368893273E-4	0.0	1.3051683256944384E-4	0.14129586558307589	6.173653350110202E-4
78-79	0.0017340093469940397	5.717880283994684E-4	0.0	1.4087531134479654E-4	0.1437425382698142	6.173653350110202E-4
80-81	0.0017671564790751686	5.759314199096094E-4	0.0	1.4294700709986707E-4	0.14627000709100024	6.235804222762319E-4
82-83	0.0018127337856867203	5.82146507174821E-4	0.0	1.4501870285493762E-4	0.14896942665985713	6.256521180313024E-4
84-85	0.0018831714413591184	5.945766817052442E-4	0.0	1.4709039861000818E-4	0.15142645782537079	6.256521180313024E-4
86-87	0.0019639675758068697	6.049351604805969E-4	0.0	1.491620943650787E-4	0.1535644478446036	6.256521180313024E-4
88-89	0.0020281901442140565	6.132219435008792E-4	0.0	1.533054858752198E-4	0.155808094347345	6.297955095414434E-4
90-91	0.0020530504932749025	6.194370307660907E-4	0.0	1.533054858752198E-4	0.15867324957660753	6.360105968066551E-4
92-93	0.0020758391465806785	6.215087265211613E-4	0.0	1.533054858752198E-4	0.1613333069261181	6.422256840718666E-4
94-95	0.0021524918895182883	6.235804222762319E-4	0.0	1.533054858752198E-4	0.16436626951154137	6.505124670921488E-4
96-97	0.002227072936700828	6.339389010515845E-4	0.0	1.533054858752198E-4	0.16693724394358392	6.505124670921488E-4
98-99	0.0023140841584137904	6.339389010515845E-4	0.0	1.533054858752198E-4	0.16891571338967626	6.546558586022898E-4
100-101	0.0024238840334325287	6.339389010515845E-4	0.0	1.533054858752198E-4	0.1709376884466251	6.587992501124309E-4
102-103	0.0024673896442890106	6.339389010515845E-4	0.0	1.533054858752198E-4	0.172814644800719	6.62942641622572E-4
104-105	0.0025253971254309854	6.339389010515845E-4	0.0	1.5537718163029032E-4	0.1746605257184869	6.67086033132713E-4
106-107	0.0026227668259193003	6.422256840718666E-4	2.0716957550705375E-6	1.6159226889550193E-4	0.17630959553952302	6.712294246428542E-4
108-109	0.002726351613672827	6.422256840718666E-4	4.143391510141075E-6	1.65735660405643E-4	0.17802703132047648	6.712294246428542E-4
110-111	0.002798860965100296	6.587992501124309E-4	4.143391510141075E-6	1.65735660405643E-4	0.17968231622877787	6.712294246428542E-4
112-113	0.00285272505473213	6.62942641622572E-4	4.143391510141075E-6	1.65735660405643E-4	0.18142254066303712	6.712294246428542E-4
114-115	0.0029128042316291756	6.712294246428542E-4	4.143391510141075E-6	1.7609413918099568E-4	0.18341758367517003	6.712294246428542E-4
116-117	0.002960453233995798	6.774445119080658E-4	4.143391510141075E-6	1.9681109673170107E-4	0.18520131372028578	6.712294246428542E-4
118-119	0.0030184607151377734	6.89874686438489E-4	4.143391510141075E-6	2.0302618399691268E-4	0.18667428940214092	6.712294246428542E-4
120-121	0.003153120939217358	6.919463821935595E-4	4.143391510141075E-6	2.154563585273359E-4	0.18815555186701638	6.712294246428542E-4
122-123	0.003310569816602719	6.9401807794863E-4	4.143391510141075E-6	2.2167144579254752E-4	0.18966167468095263	6.753728161529953E-4
124-125	0.0033913659510504696	6.981614694587712E-4	4.143391510141075E-6	2.2581483730268857E-4	0.19116572579913382	6.753728161529953E-4
126-127	0.003426584778886669	7.043765567239827E-4	4.143391510141075E-6	2.2788653305775913E-4	0.19257862230409195	6.753728161529953E-4
128-129	0.0034783771727634324	7.043765567239827E-4	4.143391510141075E-6	2.2788653305775913E-4	0.19401430746235582	6.795162076631363E-4
130-131	0.0035467431326807602	7.043765567239827E-4	4.143391510141075E-6	2.2788653305775913E-4	0.19548313975270085	6.795162076631363E-4
132-133	0.003635826050148793	7.064482524790532E-4	4.143391510141075E-6	2.320299245679002E-4	0.19674065907602867	6.795162076631363E-4
134-135	0.0037953466232892247	7.085199482341238E-4	4.143391510141075E-6	2.320299245679002E-4	0.1979132388733986	6.857312949283479E-4
136-137	0.0039320785431238795	7.168067312544059E-4	4.143391510141075E-6	2.320299245679002E-4	0.19909824884529895	6.919463821935595E-4
138-139	0.003952795500674585	7.292369057848292E-4	4.143391510141075E-6	2.320299245679002E-4	0.20005951567565167	6.960897737037006E-4
>>END_MODULE
