##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.4d.R.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24134818
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	63-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.003155358370634	37.0	35.0	38.0	33.0	39.0
2	35.33198195238099	35.0	34.0	37.0	33.0	39.0
3	35.46140215351945	35.0	34.0	37.0	33.0	39.0
4	35.522712373468075	36.0	35.0	38.0	33.0	39.0
5	35.43813141661147	36.0	35.0	37.0	33.0	39.0
6	35.39268707143348	35.0	35.0	37.0	33.0	39.0
7	35.41698731682998	35.0	34.0	37.0	33.0	39.0
8	35.547333068763976	36.0	35.0	38.0	33.0	39.0
9	35.50851711415433	36.0	34.0	38.0	33.0	39.0
10-14	35.48645272568452	35.8	34.4	37.8	33.0	39.0
15-19	35.46540105668085	36.0	34.8	38.0	33.0	39.0
20-24	35.44757612010996	36.0	34.2	38.0	33.0	39.0
25-29	35.42902156544126	36.0	34.0	38.0	33.0	39.0
30-34	35.410161849987844	36.0	34.0	38.0	33.0	39.0
35-39	35.38252755831844	36.0	34.0	38.0	33.0	39.0
40-44	35.363841119497984	36.0	34.0	38.0	33.0	39.0
45-49	35.334665801084554	36.0	34.0	38.0	32.4	39.0
50-54	35.309855147861484	36.0	34.0	38.0	32.0	39.0
55-59	35.286650937247586	36.0	34.0	38.0	32.0	39.0
60-64	35.24478566597237	36.0	34.0	38.0	32.0	39.0
65-69	35.21741982051334	36.0	34.0	38.0	32.0	39.0
70-74	35.18275870465959	35.8	34.0	38.0	32.0	39.0
75-79	35.139493880311825	35.6	34.0	37.8	32.0	39.0
80-84	35.09967294004891	35.2	34.0	37.4	32.0	39.0
85-89	35.04948957271148	35.0	34.0	37.0	32.0	39.0
90-94	34.99229025588504	35.0	34.0	37.0	31.6	39.0
95-99	34.92757391978751	35.0	34.0	37.0	31.0	39.0
100-104	34.880254404678816	35.0	34.0	37.0	31.0	39.0
105-109	34.81684650873571	35.0	34.0	37.0	31.0	39.0
110-114	34.74778347873972	35.0	34.0	37.0	31.0	39.0
115-119	34.67744914267311	35.0	34.0	37.0	30.4	39.0
120-124	34.60276738720305	35.0	34.0	37.0	30.0	39.0
125-129	34.5241066703678	35.0	34.0	37.0	30.0	39.0
130-134	34.437730495890534	35.0	34.0	37.0	30.0	39.0
135-139	34.35929400999177	35.0	34.0	37.0	29.8	39.0
140-144	34.23222996027608	35.0	34.0	37.0	29.0	39.0
145-149	34.219109553578576	35.0	34.0	37.0	29.0	39.0
150	35.01775223656289	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	9.0
21	213.0
22	1790.0
23	9985.0
24	33697.0
25	81946.0
26	156450.0
27	261285.0
28	378783.0
29	498825.0
30	639981.0
31	831211.0
32	1106931.0
33	1543194.0
34	2313969.0
35	3956589.0
36	1.0602119E7
37	1717834.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.343723578110264	18.88148068901949	16.335267164641557	31.439528568228692
2	24.379065133202992	25.494428837209384	33.356729684060596	16.769776345527028
3	19.761599196646106	29.583500484652504	30.212102697439025	20.44279762126236
4	23.40243460713066	34.15386848991362	22.482348944997224	19.9613479579585
5	25.100698915566717	35.2338061965083	22.546811830112	17.11868305781299
6	18.82298428767932	38.90659958571057	22.98029344990296	19.290122676707153
7	20.233233165462444	20.60315515948784	38.23297528077486	20.93063639427486
8	21.47717873820304	23.25256399281735	28.48843111226279	26.78182615671682
9	23.496290711618375	23.267732949135976	28.77641753917515	24.459558800070504
10-14	23.124745336799307	29.224281699576103	25.686629996546902	21.964342967077688
15-19	22.956109302336568	28.318104574063913	27.171423459667277	21.55436266393225
20-24	23.06355407362094	28.37381081556115	27.0750929217697	21.487542189048202
25-29	23.163312853654002	28.35593788194301	26.98629175492436	21.49445750947863
30-34	23.15704804569067	28.146384198961023	27.15730278140071	21.539264973947596
35-39	23.257121916205225	28.109721217783544	27.12730319430876	21.505853671702464
40-44	23.407001453253137	27.86622132389811	27.257546338240463	21.469230884608287
45-49	23.407903055245747	27.7050740552508	27.32983028916978	21.557192600333675
50-54	23.491094069986357	27.66761613864252	27.28759669950691	21.553693091864208
55-59	23.65487570695582	27.551717191320858	27.195460102495904	21.597946999227425
60-64	23.590174553463374	27.516414294342955	27.24409154018218	21.649319612011485
65-69	23.66564250298345	27.42374811755334	27.22799603924853	21.68261334021468
70-74	23.726225777130274	27.37952464581096	27.133628214918517	21.76062136214025
75-79	23.643372360820067	27.428729862833308	27.21679396830565	21.711103808040978
80-84	23.760295888507756	27.472848983624722	27.083370917596007	21.683484210271516
85-89	23.79311166658308	27.382467145222318	27.149473820708664	21.67494736748594
90-94	23.737997415377734	27.32024625055124	27.195209171551454	21.746547162519573
95-99	23.799057058842482	27.456855590800227	27.170627261289326	21.573460089067968
100-104	23.96812464862313	27.41185827313518	26.96450165478615	21.655515423455533
105-109	23.743828774938017	27.54531070597358	27.084797670636075	21.62606284845233
110-114	23.784938098764428	27.560924035200845	27.003720302193905	21.650417563840822
115-119	23.857934115757892	27.553235228135375	27.0319687805021	21.556861875604636
120-124	23.836103623833267	27.448989352998908	27.217057187987574	21.497849835180247
125-129	23.824659355029542	27.520917162859888	27.157940306564143	21.496483175546427
130-134	23.864327739296904	27.45046972410128	27.229612754232647	21.455589782369174
135-139	23.80143261219157	27.45971177153807	27.31402000756188	21.424835608708477
140-144	23.81653915329017	27.5823331111923	27.27801620731025	21.32311152820728
145-149	23.545566160913427	27.623654173756908	27.282985913605717	21.54779375172395
150	23.66802548307039	26.409950081363647	27.705669868418664	22.2163545671473
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	2.0
9	1.5
10	3.5
11	6.0
12	8.5
13	13.0
14	25.5
15	48.0
16	81.5
17	145.5
18	248.5
19	441.0
20	666.5
21	1022.5
22	1597.5
23	2670.5
24	4368.5
25	7214.0
26	11449.0
27	18187.0
28	29754.5
29	45670.0
30	61191.5
31	79531.0
32	104773.0
33	137306.5
34	184846.0
35	255435.5
36	355553.5
37	481969.5
38	630151.5
39	815289.0
40	1030481.5
41	1228310.0
42	1408418.5
43	1577753.5
44	1696479.0
45	1747505.5
46	1743396.5
47	1686723.0
48	1579651.5
49	1430258.5
50	1242185.5
51	1041880.0
52	849533.0
53	674174.5
54	526508.5
55	399858.5
56	297082.5
57	220270.0
58	160733.0
59	114615.5
60	78857.5
61	52859.0
62	35370.0
63	23944.0
64	16393.0
65	11236.0
66	7907.0
67	6117.0
68	5554.0
69	4193.0
70	2392.0
71	1677.5
72	967.5
73	702.5
74	581.5
75	358.0
76	245.0
77	91.5
78	22.5
79	1.5
80	1.0
81	0.5
82	1.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	8.286783020282149E-7
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	8.286784325027038E-7
70-74	0.0
75-79	0.0
80-84	8.286837819921246E-7
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	8.287333453085302E-7
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	3.3158864101330036E-6
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62-63	2.0
64-65	0.0
66-67	3.0
68-69	4.0
70-71	11.0
72-73	19.0
74-75	13.0
76-77	28.0
78-79	35.0
80-81	44.0
82-83	49.0
84-85	60.0
86-87	74.0
88-89	88.0
90-91	114.0
92-93	88.0
94-95	95.0
96-97	133.0
98-99	156.0
100-101	163.0
102-103	175.0
104-105	156.0
106-107	187.0
108-109	199.0
110-111	204.0
112-113	214.0
114-115	224.0
116-117	252.0
118-119	266.0
120-121	316.0
122-123	306.0
124-125	361.0
126-127	489.0
128-129	514.0
130-131	659.0
132-133	758.0
134-135	1137.0
136-137	1642.0
138-139	3545.0
140-141	7296.0
142-143	11489.0
144-145	71953.0
146-147	840197.0
148-149	1054678.0
150-151	2.2136422E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.38007371651909
#Duplication Level	Percentage of total
1	28.99277064488762
2	10.777004778616856
3	7.047679811319699
4	5.02629897960693
5	3.9664098829028163
6	3.138486144577802
7	2.5488946986232657
8	2.104691526948774
9	1.8509237291895242
>10	20.537248970819597
>50	5.309001065880759
>100	6.4867574159666015
>500	1.0994800113351277
>1k	0.9781805601708617
>5k	0.0800877236190488
>10k+	0.056084055534731135
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.65735660405643E-5	1.2430174530423225E-5	0.0	0.0	6.132219435008792E-4	0.0
2	3.7290523591269676E-5	4.557730661155182E-5	0.0	0.0	0.004922349114047597	0.0
3	7.043765567239827E-5	4.557730661155182E-5	0.0	0.0	0.006509268062431629	0.0
4	1.160149622839501E-4	4.557730661155182E-5	0.0	0.0	0.008038179529673686	0.0
5	1.2430174530423226E-4	4.97206981216929E-5	4.143391510141075E-6	0.0	0.009645815435608423	0.0
6	1.325885283245144E-4	4.97206981216929E-5	4.143391510141075E-6	0.0	0.011220304209462031	0.0
7	1.65735660405643E-4	5.800748114197505E-5	4.143391510141075E-6	0.0	0.013325147096613697	0.0
8	2.444600990983234E-4	5.800748114197505E-5	4.143391510141075E-6	0.0	0.018985019899466406	0.0
9	2.6103366513888774E-4	5.800748114197505E-5	4.143391510141075E-6	0.0	0.02505923185333322	0.0
10-11	2.9003740570987524E-4	6.007917689704559E-5	1.2430174530423225E-5	4.143391510141075E-6	0.03156642822000978	0.0
12-13	3.211128420359333E-4	6.215087265211613E-5	1.2430174530423225E-5	4.143391510141075E-6	0.03773800987436491	0.0
14-15	3.832637146880494E-4	7.872443869268042E-5	1.2430174530423225E-5	6.215087265211612E-6	0.046292041647051155	0.0
16-17	4.309127170546718E-4	7.872443869268042E-5	1.4501870285493761E-5	8.28678302028215E-6	0.05201613701831105	0.0
18-19	5.220673302777755E-4	8.28678302028215E-5	1.65735660405643E-5	8.28678302028215E-6	0.05979949797011107	0.0
20-21	6.152936392559497E-4	8.908291746803312E-5	1.65735660405643E-5	8.28678302028215E-6	0.06581155905132577	0.0
22-23	6.815879034182068E-4	1.0151309199845634E-4	1.65735660405643E-5	8.28678302028215E-6	0.07213437449580104	0.0
24-25	7.499538633355346E-4	1.0979987501873848E-4	1.65735660405643E-5	8.28678302028215E-6	0.07721624418298907	0.0
26-27	7.893160826818748E-4	1.1187157077380902E-4	1.65735660405643E-5	1.0358478775352688E-5	0.0813492772143548	0.0
28-29	9.902705709237168E-4	1.1187157077380902E-4	1.65735660405643E-5	1.2430174530423225E-5	0.08585521548163322	0.0
30-31	0.0012347306700220403	1.3466022407958495E-4	2.0716957550705376E-5	1.2430174530423225E-5	0.09093915686457632	0.0
32-33	0.0014336134625088119	1.4709039861000818E-4	2.0716957550705376E-5	1.2430174530423225E-5	0.09598995111543829	0.0
34-35	0.0016138509931999487	1.5744887738536084E-4	2.0716957550705376E-5	1.2430174530423225E-5	0.0990125552220862	0.0
36-37	0.0016635716913216416	1.7402244342592515E-4	2.9003740570987526E-5	1.2430174530423225E-5	0.10238320421558597	2.0716957550705375E-6
38-39	0.0017091489979331933	1.7609413918099568E-4	2.9003740570987526E-5	1.2430174530423225E-5	0.10564819672557713	4.143391510141075E-6
40-41	0.0017754432620954506	1.8023753069113676E-4	2.9003740570987526E-5	1.4501870285493761E-5	0.10849263499728898	4.143391510141075E-6
42-43	0.0017940885238910856	1.9059600946648946E-4	2.9003740570987526E-5	1.65735660405643E-5	0.11161260880442521	4.143391510141075E-6
44-45	0.0018562393965432017	2.2374314154761807E-4	2.9003740570987526E-5	1.65735660405643E-5	0.11399505892275633	4.143391510141075E-6
46-47	0.0019266770522155998	2.8175062268959313E-4	2.9003740570987526E-5	1.65735660405643E-5	0.11651424096092211	4.143391510141075E-6
48-49	0.001992971316377857	2.9418079722001635E-4	2.9003740570987526E-5	1.65735660405643E-5	0.11883661190235617	4.143391510141075E-6
50-51	0.0020364769272343385	3.04539275995369E-4	2.9003740570987526E-5	1.65735660405643E-5	0.12096010005130348	8.28678302028215E-6
52-53	0.0020882693211111015	3.211128420359333E-4	2.9003740570987526E-5	2.0716957550705376E-5	0.12319545977102458	1.0358478775352688E-5
54-55	0.0021462768022530773	3.4390149534170925E-4	2.9003740570987526E-5	2.0716957550705376E-5	0.12516149904258653	1.2430174530423225E-5
56-57	0.002231216328210969	3.4597319109677975E-4	2.9003740570987526E-5	2.0716957550705376E-5	0.12710889305235284	1.2430174530423225E-5
58-59	0.0022912955051080145	3.542599741170619E-4	2.9003740570987526E-5	2.0716957550705376E-5	0.12891541175077434	1.2430174530423225E-5
60-61	0.002378306726820977	3.708335401576262E-4	2.9003740570987526E-5	2.0716957550705376E-5	0.13044432321801638	1.2430174530423225E-5
62-63	0.0024176689461673172	3.811920189329789E-4	2.9003740570987526E-5	2.278865330577591E-5	0.1323067777018248	1.2430174530423225E-5
64-65	0.0024487443824933756	3.9569388921847264E-4	2.9003740570987526E-5	2.486034906084645E-5	0.1339952097422073	1.2430174530423225E-5
66-67	0.0025419706914715495	4.101957595039664E-4	2.9003740570987526E-5	2.486034906084645E-5	0.13530866485092202	1.2430174530423225E-5
68-69	0.0025606159532671844	4.164108467691781E-4	2.9003740570987526E-5	2.9003740570987526E-5	0.13667598404926856	1.2430174530423225E-5
70-71	0.0025916913895932423	4.226259340343897E-4	3.7290523591269676E-5	3.31471320811286E-5	0.1380888805542267	1.2430174530423225E-5
72-73	0.0026455554792250762	4.4334289158509503E-4	3.7290523591269676E-5	3.7290523591269676E-5	0.1389092720732346	1.2430174530423225E-5
74-75	0.0026662724367757817	4.4541458734016553E-4	4.143391510141075E-5	3.7290523591269676E-5	0.13995547842954523	1.2430174530423225E-5
76-77	0.002713921439142404	4.5784476187058876E-4	4.143391510141075E-5	3.7290523591269676E-5	0.1412730769297701	1.2430174530423225E-5
78-79	0.002759498745753956	4.744183279111531E-4	4.143391510141075E-5	3.7290523591269676E-5	0.1428413506163585	1.2430174530423225E-5
80-81	0.00277607231179452	4.889201981966468E-4	4.557730661155182E-5	3.7290523591269676E-5	0.14418173776988913	1.2430174530423225E-5
82-83	0.002794717573590155	5.054937642372111E-4	4.557730661155182E-5	3.7290523591269676E-5	0.14571272093288623	1.2430174530423225E-5
84-85	0.002821649618406072	5.096371557473521E-4	4.97206981216929E-5	3.7290523591269676E-5	0.14738457940722816	1.65735660405643E-5
86-87	0.0028506533589770598	5.137805472574933E-4	4.97206981216929E-5	3.936221934634021E-5	0.1491206604499773	1.65735660405643E-5
88-89	0.0028568684462422713	5.179239387676344E-4	5.3864089631833976E-5	4.143391510141075E-5	0.1508277377521554	1.65735660405643E-5
90-91	0.0028672269250176238	5.262107217879165E-4	5.3864089631833976E-5	4.350561085648129E-5	0.15176207253769222	1.8645261795634838E-5
92-93	0.0028775854037929767	5.303541132980576E-4	5.593578538690452E-5	4.557730661155182E-5	0.1527709883704116	2.0716957550705376E-5
94-95	0.0029045174486088936	5.427842878284808E-4	5.800748114197505E-5	5.3864089631833976E-5	0.15377990420313092	2.486034906084645E-5
96-97	0.002910732535874105	5.531427666038335E-4	5.800748114197505E-5	5.3864089631833976E-5	0.15458786554760845	2.486034906084645E-5
98-99	0.002921091014649458	5.593578538690451E-4	6.215087265211613E-5	5.3864089631833976E-5	0.1554206872411468	2.9003740570987526E-5
100-101	0.002921091014649458	5.593578538690451E-4	6.215087265211613E-5	5.3864089631833976E-5	0.15621000332382867	2.9003740570987526E-5
102-103	0.002933521189179881	5.593578538690451E-4	6.215087265211613E-5	5.3864089631833976E-5	0.1570656136706728	2.9003740570987526E-5
104-105	0.0029418079722001635	5.635012453791862E-4	6.215087265211613E-5	5.3864089631833976E-5	0.15789429197270102	2.9003740570987526E-5
106-107	0.0029625249297508685	5.697163326443978E-4	6.215087265211613E-5	5.3864089631833976E-5	0.15870846840444375	2.9003740570987526E-5
108-109	0.0029728834085262215	5.987200732153854E-4	6.62942641622572E-5	5.3864089631833976E-5	0.15952678822769661	3.1075436326058065E-5
110-111	0.002997743757587068	6.152936392559497E-4	6.62942641622572E-5	5.3864089631833976E-5	0.16040933061935664	3.7290523591269676E-5
112-113	0.0030039588448522794	6.277238137863729E-4	6.62942641622572E-5	5.593578538690452E-5	0.16120693348505882	4.143391510141075E-5
114-115	0.0030039588448522794	6.380822925617256E-4	6.62942641622572E-5	6.215087265211613E-5	0.162064615527658	4.143391510141075E-5
116-117	0.0030039588448522794	6.422256840718666E-4	6.62942641622572E-5	6.215087265211613E-5	0.16299895031319483	4.350561085648129E-5
118-119	0.003014317323627632	6.608709458675015E-4	7.043765567239827E-5	6.215087265211613E-5	0.1637799796128564	4.557730661155182E-5
120-121	0.0030163890193827024	6.62942641622572E-4	7.043765567239827E-5	6.215087265211613E-5	0.1646542352214962	4.557730661155182E-5
122-123	0.0030226041066479144	6.62942641622572E-4	7.043765567239827E-5	6.215087265211613E-5	0.16541454756360707	4.557730661155182E-5
124-125	0.0030267474981580554	6.650143373776425E-4	7.043765567239827E-5	6.215087265211613E-5	0.16628051638922656	4.557730661155182E-5
126-127	0.0030371059769334083	6.753728161529953E-4	7.043765567239827E-5	6.215087265211613E-5	0.16731429257100675	4.557730661155182E-5
128-129	0.0030578229344841133	6.815879034182069E-4	7.043765567239827E-5	6.215087265211613E-5	0.16835635553580724	4.557730661155182E-5
130-131	0.0030743965005246773	7.085199482341238E-4	7.043765567239827E-5	6.215087265211613E-5	0.16936527136852658	4.97206981216929E-5
132-133	0.003099256849585524	7.20950122764547E-4	7.043765567239827E-5	6.215087265211613E-5	0.17017116101724902	5.3864089631833976E-5
134-135	0.0031116870241159472	7.395953845601819E-4	7.458104718253935E-5	6.215087265211613E-5	0.17102055627682794	5.3864089631833976E-5
136-137	0.003117902111381159	7.437387760703229E-4	7.458104718253935E-5	6.215087265211613E-5	0.171874094927917	5.3864089631833976E-5
138-139	0.0031199738071362297	7.540972548456756E-4	7.458104718253935E-5	6.215087265211613E-5	0.17275870901533213	5.3864089631833976E-5
>>END_MODULE
