##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.4d.R.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23585421
Total Bases	3.5 Gbp
Sequences flagged as poor quality	0
Sequence length	65-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.82667161209461	36.0	35.0	38.0	33.0	39.0
2	35.22751830463404	35.0	34.0	37.0	32.0	39.0
3	35.35108268790284	35.0	34.0	37.0	33.0	39.0
4	35.40478348891885	36.0	35.0	37.0	33.0	39.0
5	35.319985808182096	35.0	34.0	37.0	33.0	39.0
6	35.28319142575407	35.0	34.0	37.0	33.0	39.0
7	35.30748639169935	35.0	34.0	37.0	33.0	39.0
8	35.38539511336261	36.0	34.0	38.0	33.0	39.0
9	35.390219067957275	36.0	34.0	38.0	33.0	39.0
10-14	35.37072171830217	35.4	34.2	37.4	33.0	39.0
15-19	35.34734654937896	35.8	34.0	37.2	33.0	39.0
20-24	35.32992889972157	35.6	34.0	37.0	32.8	39.0
25-29	35.310903502634105	35.8	34.0	37.0	32.0	39.0
30-34	35.289958190697554	35.6	34.0	37.0	32.0	39.0
35-39	35.26398562908841	35.4	34.0	37.0	32.0	39.0
40-44	35.241653833527074	35.4	34.0	37.0	32.0	39.0
45-49	35.213079741082424	35.0	34.0	37.0	32.0	39.0
50-54	35.1873926015567	35.0	34.0	37.0	32.0	39.0
55-59	35.16108284011551	35.0	34.0	37.0	32.0	39.0
60-64	35.116919736137	35.0	34.0	37.0	32.0	39.0
65-69	35.08965258098419	35.0	34.0	37.0	32.0	39.0
70-74	35.055804063141494	35.0	34.0	37.0	32.0	39.0
75-79	35.00699501315463	35.0	34.0	37.0	32.0	39.0
80-84	34.96458427575857	35.0	34.0	37.0	31.4	39.0
85-89	34.912511574408384	35.0	34.0	37.0	31.0	39.0
90-94	34.855176210828176	35.0	34.0	37.0	31.0	39.0
95-99	34.7883776507514	35.0	34.0	37.0	31.0	39.0
100-104	34.73694081112045	35.0	34.0	37.0	31.0	39.0
105-109	34.67364080988686	35.0	34.0	37.0	30.8	39.0
110-114	34.59731538457101	35.0	34.0	37.0	30.0	39.0
115-119	34.53244016633745	35.0	34.0	37.0	30.0	39.0
120-124	34.45610192997047	35.0	34.0	37.0	30.0	39.0
125-129	34.37570520003438	35.0	34.0	37.0	30.0	39.0
130-134	34.27328022353512	35.0	34.0	37.0	29.6	39.0
135-139	34.211272914941304	35.0	34.0	37.0	29.0	39.0
140-144	34.08626257977247	35.0	34.0	37.0	29.0	39.0
145-149	34.07995914018509	35.0	34.0	37.0	29.0	39.0
150	34.910289180520046	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	11.0
21	181.0
22	1779.0
23	9675.0
24	33957.0
25	84599.0
26	165550.0
27	277487.0
28	404423.0
29	530021.0
30	681442.0
31	880659.0
32	1172157.0
33	1624439.0
34	2410552.0
35	4055345.0
36	9797323.0
37	1455818.0
38	3.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.32405306651088	19.523933874235276	17.186519587672404	30.96549347158145
2	23.678025505671492	25.849468618770892	33.23180875168605	17.240697123871566
3	19.831064283312987	29.690981560176517	30.320930035550354	20.15702412096015
4	23.187840045913113	34.261487330858415	22.44740373055928	20.103268892669192
5	24.949880036015482	35.2786290101695	22.61523775798362	17.15625319583139
6	18.82728856797086	38.91085893713961	22.974507993146435	19.287344501743092
7	20.1880076578187	20.57850095213931	38.37277458212041	20.86071680792158
8	21.471139670605364	23.114712784751468	28.575436256943256	26.83871128769992
9	23.455871319829313	23.10620616015292	28.81947284299059	24.618449677027176
10-14	22.982328786923073	29.06576651737529	25.872509123326655	22.079395572374985
15-19	22.825210540019615	28.24550895233119	27.295364369370382	21.63391613827881
20-24	22.91519239788003	28.387124401976966	27.124238316543085	21.573444883599915
25-29	23.035641382021545	28.340782214572297	27.042027360885353	21.581549042520802
30-34	23.046220798857057	28.15296703840902	27.173911375166888	21.62690078756703
35-39	23.152966598755786	28.10923008844515	27.1329100051503	21.604893307648766
40-44	23.274780636733176	27.88895394320076	27.263755012047486	21.57251040801858
45-49	23.30126140211786	27.711858100815757	27.329133535500596	21.657746961565792
50-54	23.37818349733931	27.665684661723866	27.296218286711948	21.659913554224875
55-59	23.53879393154605	27.56228712272965	27.197907785473983	21.70101116025032
60-64	23.46933641761154	27.522827767204156	27.249915106454957	21.757920708729344
65-69	23.541020532958466	27.431119981526216	27.228758502033052	21.79910098348227
70-74	23.608710673626145	27.385020626192652	27.13379062584303	21.872478074338176
75-79	23.537373535913307	27.451475814310513	27.208212962011334	21.80293768776485
80-84	23.650370056954298	27.47381803451201	27.081941945320136	21.793869963213552
85-89	23.67499770660278	27.39677138855149	27.15559961953604	21.77263128530969
90-94	23.614612607081497	27.336841801767576	27.19779061464907	21.850754976501854
95-99	23.700113778030147	27.45363038583193	27.177846076820394	21.66840975931753
100-104	23.853970679329766	27.42718456276053	26.978232338015	21.7406124198947
105-109	23.62699837849754	27.57127883715194	27.075076441622798	21.726646342727722
110-114	23.66214506773916	27.566312887606625	27.002858179318594	21.76868386533562
115-119	23.741619943370786	27.56035285205991	27.02068031760302	21.67734688696629
120-124	23.71567696428555	27.459905974737186	27.20967271974523	21.61474434123203
125-129	23.69508005316791	27.53490517060806	27.164895269497286	21.605119506726744
130-134	23.729191028400383	27.448180858591908	27.24654146225892	21.576086650748785
135-139	23.673190853032946	27.45289434291002	27.326051493224274	21.54786331083276
140-144	23.69059224615516	27.55471316719665	27.31002401322855	21.444670573419643
145-149	23.39468846443644	27.587373518358092	27.340273255619067	21.677664761586396
150	23.50423896921488	26.417612591730123	27.771513260800933	22.306635178254062
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	4.0
9	5.0
10	3.5
11	5.0
12	12.0
13	17.0
14	23.5
15	42.0
16	81.0
17	157.5
18	253.0
19	429.5
20	658.0
21	1027.0
22	1628.5
23	2712.5
24	4438.0
25	7132.0
26	11353.0
27	18265.5
28	30340.5
29	46049.0
30	61178.5
31	79616.5
32	105045.0
33	137799.5
34	184930.5
35	255106.5
36	354438.0
37	478130.0
38	624230.0
39	806604.5
40	1015086.5
41	1207729.5
42	1382858.0
43	1544345.5
44	1654944.5
45	1701827.0
46	1696054.0
47	1636974.0
48	1529821.0
49	1384187.0
50	1202147.0
51	1007403.0
52	823977.5
53	658298.0
54	515593.5
55	393108.0
56	292952.5
57	216476.5
58	157231.0
59	112400.5
60	77738.5
61	51567.0
62	34350.0
63	23697.5
64	16080.5
65	10603.0
66	7371.5
67	5466.0
68	4605.0
69	3392.5
70	1971.5
71	1306.5
72	702.0
73	498.5
74	446.5
75	317.0
76	205.0
77	75.5
78	22.5
79	4.0
80	1.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	1.2719722068984904E-5
5	3.815916620695471E-5
6	1.1871740597719242E-4
7	0.002700820985981128
8	0.11496084805948557
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	8.479814712656602E-7
40-44	0.0
45-49	0.0
50-54	0.0
55-59	8.479814712656602E-7
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	1.6960569736744907E-6
105-109	4.9186525676939525E-5
110-114	0.019453708256386492
115-119	8.480830020869542E-7
120-124	0.0
125-129	5.088844481935657E-6
130-134	0.048285811699227736
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
64-65	1.0
66-67	5.0
68-69	4.0
70-71	8.0
72-73	19.0
74-75	18.0
76-77	27.0
78-79	30.0
80-81	41.0
82-83	70.0
84-85	59.0
86-87	75.0
88-89	104.0
90-91	120.0
92-93	114.0
94-95	136.0
96-97	141.0
98-99	168.0
100-101	176.0
102-103	148.0
104-105	162.0
106-107	195.0
108-109	222.0
110-111	202.0
112-113	231.0
114-115	231.0
116-117	225.0
118-119	270.0
120-121	282.0
122-123	352.0
124-125	362.0
126-127	454.0
128-129	470.0
130-131	646.0
132-133	841.0
134-135	1100.0
136-137	1658.0
138-139	3494.0
140-141	7197.0
142-143	11363.0
144-145	71048.0
146-147	818810.0
148-149	1095573.0
150-151	2.1568569E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.935731417959694
#Duplication Level	Percentage of total
1	29.464072682679543
2	10.782940846853382
3	7.136499408567013
4	5.179187456072016
5	4.009265422705196
6	3.155328218833818
7	2.507738453691636
8	2.162816297150087
9	1.857581540290217
>10	20.605186736806502
>50	5.106616556964324
>100	5.9899359781194725
>500	0.9936780155301321
>1k	0.9312437433413648
>5k	0.06425931317884791
>10k+	0.05364932921624791
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	8.098223050587056E-4	0.0
2	0.0	0.0	0.0	0.0	0.005109088364375603	0.0
3	2.5439444137969808E-5	8.479814712656603E-6	0.0	0.0	0.006741452696561999	0.0
4	8.479814712656603E-5	8.479814712656603E-6	0.0	4.239907356328301E-6	0.00821270054920792	0.0
5	8.903805448289432E-5	8.479814712656603E-6	0.0	4.239907356328301E-6	0.009925623121164554	0.0
6	8.903805448289432E-5	8.479814712656603E-6	0.0	4.239907356328301E-6	0.011647025507833843	0.0
7	9.327796183922263E-5	2.5439444137969808E-5	0.0	8.479814712656603E-6	0.013843297518411903	0.0
8	1.4415685011516223E-4	2.967935149429811E-5	0.0	8.479814712656603E-6	0.01921950004623619	0.0
9	1.4415685011516223E-4	4.2399073563283013E-5	0.0	8.479814712656603E-6	0.025414004693831836	0.0
10-11	1.759561552876245E-4	4.2399073563283013E-5	0.0	8.479814712656603E-6	0.031964661559359064	0.0
12-13	2.1623527517274338E-4	4.2399073563283013E-5	0.0	8.479814712656603E-6	0.03824608430775944	0.0
14-15	2.628742560923547E-4	4.6638980919611315E-5	0.0	8.479814712656603E-6	0.047232567949497276	0.0
16-17	2.9891346862114524E-4	5.511879563226792E-5	0.0	8.479814712656603E-6	0.05330623523743757	0.0
18-19	3.476724032189207E-4	5.723874931043207E-5	0.0	8.479814712656603E-6	0.06091262903469054	0.0
20-21	3.9007147678220375E-4	5.935870298859622E-5	0.0	8.479814712656603E-6	0.06696933669320551	0.0
22-23	4.3247055034548675E-4	8.055823977023772E-5	0.0	8.479814712656603E-6	0.07371290934344568	0.0
24-25	4.79109531265098E-4	8.691810080473017E-5	0.0	8.479814712656603E-6	0.07930746710012088	0.0
26-27	5.066689290812319E-4	9.751786919555092E-5	0.0	8.479814712656603E-6	0.08343289695782832	0.0
28-29	6.084267056331112E-4	1.0175777655187923E-4	0.0	8.479814712656603E-6	0.08798443750484675	0.0
30-31	8.03462444024213E-4	1.1023759126453582E-4	4.239907356328301E-6	8.479814712656603E-6	0.09308716600818785	0.0
32-33	9.942582750589867E-4	1.1871740597719244E-4	4.239907356328301E-6	1.2719722068984904E-5	0.09819201446520713	0.0
34-35	0.0011468949398868054	1.2507726701168487E-4	4.239907356328301E-6	1.4839675747149055E-5	0.10137618488980968	0.0
36-37	0.0011935339208064169	1.3143712804617735E-4	4.239907356328301E-6	1.6959629425313205E-5	0.10493346716176913	0.0
38-39	0.0012359329943696998	1.3143712804617735E-4	4.239907356328301E-6	1.6959629425313205E-5	0.10837415198142955	0.0
40-41	0.001280452021611147	1.3779698908066978E-4	4.239907356328301E-6	1.6959629425313205E-5	0.11124244930798563	0.0
42-43	0.0013037715120709527	1.5263666482781882E-4	4.239907356328301E-6	1.6959629425313205E-5	0.11428246288247303	0.0
44-45	0.0013419306782779072	1.632364332186396E-4	4.239907356328301E-6	1.6959629425313205E-5	0.11682640729627002	0.0
46-47	0.001407649242300996	1.8867587735660942E-4	4.239907356328301E-6	1.6959629425313205E-5	0.11935339208064169	0.0
48-49	0.0014691278989677563	2.0139559942559433E-4	4.239907356328301E-6	1.6959629425313205E-5	0.12182949797673741	0.0
50-51	0.0015348464629908452	2.0987541413825092E-4	4.239907356328301E-6	1.6959629425313205E-5	0.1239409718401889	0.0
52-53	0.0015963251196576054	2.183552288509075E-4	4.239907356328301E-6	1.6959629425313205E-5	0.12614148375812329	0.0
54-55	0.00165992373000253	2.3107495091989242E-4	4.239907356328301E-6	1.6959629425313205E-5	0.12796676387502262	0.0
56-57	0.0017828810433360505	2.39554765632549E-4	4.239907356328301E-6	1.9079583103477356E-5	0.12981112357502544	2.1199536781641507E-6
58-59	0.001842239746324647	2.565143950578622E-4	4.239907356328301E-6	2.1199536781641507E-5	0.13160672434043047	4.239907356328301E-6
60-61	0.0019376376618420337	2.6923411712684715E-4	4.239907356328301E-6	2.1199536781641507E-5	0.13316277034020296	4.239907356328301E-6
62-63	0.0019736768743708246	2.755939781613396E-4	4.239907356328301E-6	2.1199536781641507E-5	0.1350346894380219	8.479814712656603E-6
64-65	0.0020075961332214507	2.9255360758665284E-4	4.239907356328301E-6	2.1199536781641507E-5	0.13671157279744975	8.479814712656603E-6
66-67	0.0021072339560951657	3.285928201154434E-4	4.239907356328301E-6	2.1199536781641507E-5	0.1380407837536587	8.479814712656603E-6
68-69	0.002141153214945792	3.413125421844283E-4	4.239907356328301E-6	2.1199536781641507E-5	0.1394102738297527	8.479814712656603E-6
70-71	0.002162352751727434	3.476724032189207E-4	4.239907356328301E-6	2.1199536781641507E-5	0.14076704418377778	8.479814712656603E-6
72-73	0.0022005119179343887	3.6675198632239806E-4	4.239907356328301E-6	2.1199536781641507E-5	0.14183126093021617	8.479814712656603E-6
74-75	0.002208991732647045	3.7523180103505465E-4	6.359861034492452E-6	2.1199536781641507E-5	0.14286155841780396	8.479814712656603E-6
76-77	0.0022238314083941942	3.8583156942587543E-4	1.2719722068984904E-5	2.1199536781641507E-5	0.14413777053205878	8.479814712656603E-6
78-79	0.0022916699260954465	4.006712451730245E-4	1.2719722068984904E-5	2.1199536781641507E-5	0.14576165504953248	8.479814712656603E-6
80-81	0.002293789879773611	4.1551092092017357E-4	1.2719722068984904E-5	2.1199536781641507E-5	0.14702938734907467	8.479814712656603E-6
82-83	0.0023149894165552525	4.2823064298915843E-4	1.2719722068984904E-5	2.1199536781641507E-5	0.14861087279298513	1.4839675747149055E-5
84-85	0.002355268536440371	4.2823064298915843E-4	1.2719722068984904E-5	2.1199536781641507E-5	0.1501626788854013	1.9079583103477356E-5
86-87	0.002376468073222013	4.3035059666732256E-4	1.6959629425313205E-5	2.3319490459805657E-5	0.1519074007625304	2.1199536781641507E-5
88-89	0.0023997875636818187	4.3247055034548675E-4	1.9079583103477356E-5	2.5439444137969808E-5	0.1535440050020731	2.1199536781641507E-5
90-91	0.0024103873320726393	4.4095036505814334E-4	2.1199536781641507E-5	2.5439444137969808E-5	0.15456370272127007	2.1199536781641507E-5
92-93	0.0024167471931071318	4.4519027241447166E-4	2.1199536781641507E-5	2.5439444137969808E-5	0.15552616169115657	2.1199536781641507E-5
94-95	0.0024252270078197885	4.4519027241447166E-4	2.1199536781641507E-5	2.5439444137969808E-5	0.15657341880816966	2.1199536781641507E-5
96-97	0.0024252270078197885	4.515501334489641E-4	2.1199536781641507E-5	2.5439444137969808E-5	0.1574108005110445	2.1199536781641507E-5
98-99	0.002433706822532445	4.5367008712712825E-4	2.1199536781641507E-5	3.391925885062641E-5	0.15824394230656302	2.1199536781641507E-5
100-101	0.002435826776210609	4.579099944834565E-4	2.1199536781641507E-5	3.815916620695471E-5	0.1590092455843803	2.967935149429811E-5
102-103	0.0024379467298887734	4.6214990183978484E-4	2.3319490459805657E-5	4.0279119885118866E-5	0.15993566534173803	2.967935149429811E-5
104-105	0.0024421866372451016	4.6214990183978484E-4	2.5439444137969808E-5	4.2399073563283013E-5	0.1607815268593255	2.967935149429811E-5
106-107	0.0024803458034520563	4.6638980919611316E-4	2.5439444137969808E-5	4.2399073563283013E-5	0.1615553099518554	3.1799305172462263E-5
108-109	0.002488825618164713	4.79109531265098E-4	2.5439444137969808E-5	4.2399073563283013E-5	0.16235453248852333	3.391925885062641E-5
110-111	0.0025100251549463543	4.93949207012247E-4	2.5439444137969808E-5	4.2399073563283013E-5	0.1632851921532374	3.391925885062641E-5
112-113	0.0025291047380498317	5.045489754030678E-4	2.967935149429811E-5	4.2399073563283013E-5	0.16409501445829608	3.391925885062641E-5
114-115	0.0025375845527624884	5.130287901157245E-4	2.967935149429811E-5	4.2399073563283013E-5	0.16487515741186048	3.391925885062641E-5
116-117	0.0025397045064406523	5.172686974720528E-4	2.967935149429811E-5	4.2399073563283013E-5	0.1657740177714021	3.391925885062641E-5
118-119	0.002552424228509637	5.172686974720528E-4	3.391925885062641E-5	4.2399073563283013E-5	0.166547800863932	4.451902724144716E-5
120-121	0.0025545441821878015	5.172686974720528E-4	3.391925885062641E-5	4.451902724144716E-5	0.16746786076025524	4.875893459777547E-5
122-123	0.002560904043222294	5.172686974720528E-4	3.391925885062641E-5	4.6638980919611315E-5	0.16819500487186556	5.0878888275939616E-5
124-125	0.0025693838579349502	5.193886511502168E-4	3.391925885062641E-5	4.6638980919611315E-5	0.16910658495347614	5.2998841954103763E-5
126-127	0.002590583394716592	5.257485121847094E-4	4.0279119885118866E-5	4.6638980919611315E-5	0.17019836109773068	5.935870298859622E-5
128-129	0.0026075430241419054	5.384682342536943E-4	4.2399073563283013E-5	4.6638980919611315E-5	0.17127953747359437	5.935870298859622E-5
130-131	0.002613902885176398	5.49068002644515E-4	4.2399073563283013E-5	4.6638980919611315E-5	0.1722123170919866	6.147865666676037E-5
132-133	0.002630862514601711	5.533079100008434E-4	4.6638980919611315E-5	4.6638980919611315E-5	0.17318113592290763	6.995847137941697E-5
134-135	0.0026563019587396808	5.554278636790075E-4	4.6638980919611315E-5	4.6638980919611315E-5	0.17399519813532266	7.631833241390942E-5
136-137	0.002666901727130502	5.575478173571716E-4	4.875893459777547E-5	4.6638980919611315E-5	0.17485801928233546	7.631833241390942E-5
138-139	0.00267114163448683	5.702675394261565E-4	5.0878888275939616E-5	4.6638980919611315E-5	0.1756551218653252	7.631833241390942E-5
>>END_MODULE
