##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.4d.R.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22805127
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	63-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.93095219333793	37.0	35.0	38.0	33.0	39.0
2	35.22068046365188	35.0	34.0	37.0	33.0	39.0
3	35.38554255803969	35.0	34.0	37.0	33.0	39.0
4	35.43373439665563	36.0	35.0	37.0	33.0	39.0
5	35.346660775009056	35.0	34.0	37.0	33.0	39.0
6	35.29993097604762	35.0	34.0	37.0	33.0	39.0
7	35.3356853044493	35.0	34.0	37.0	33.0	39.0
8	35.4070204914886	36.0	34.0	38.0	33.0	39.0
9	35.41965001992754	36.0	34.0	38.0	33.0	39.0
10-14	35.40598395264363	35.4	34.2	37.6	33.0	39.0
15-19	35.37771623898433	36.0	34.0	37.6	33.0	39.0
20-24	35.36278542978515	36.0	34.0	37.8	33.0	39.0
25-29	35.33929898307516	36.0	34.0	37.6	33.0	39.0
30-34	35.32298893139249	36.0	34.0	37.6	32.4	39.0
35-39	35.29272313195187	35.8	34.0	37.0	32.0	39.0
40-44	35.27410278399239	35.8	34.0	37.0	32.0	39.0
45-49	35.24156477620143	35.4	34.0	37.0	32.0	39.0
50-54	35.21795360315249	35.2	34.0	37.0	32.0	39.0
55-59	35.18936497919964	35.0	34.0	37.0	32.0	39.0
60-64	35.1461351839401	35.0	34.0	37.0	32.0	39.0
65-69	35.11634389230549	35.0	34.0	37.0	32.0	39.0
70-74	35.08473239299628	35.0	34.0	37.0	32.0	39.0
75-79	35.036461579319266	35.0	34.0	37.0	32.0	39.0
80-84	34.99138199592554	35.0	34.0	37.0	32.0	39.0
85-89	34.940428973591914	35.0	34.0	37.0	31.2	39.0
90-94	34.88254980243288	35.0	34.0	37.0	31.0	39.0
95-99	34.81669856502799	35.0	34.0	37.0	31.0	39.0
100-104	34.76437581405024	35.0	34.0	37.0	31.0	39.0
105-109	34.70179468187037	35.0	34.0	37.0	31.0	39.0
110-114	34.6240338545311	35.0	34.0	37.0	30.0	39.0
115-119	34.56011589156517	35.0	34.0	37.0	30.0	39.0
120-124	34.48251210108542	35.0	34.0	37.0	30.0	39.0
125-129	34.40294909100923	35.0	34.0	37.0	30.0	39.0
130-134	34.30017534365916	35.0	34.0	37.0	29.8	39.0
135-139	34.238396838016634	35.0	34.0	37.0	29.0	39.0
140-144	34.11233534742897	35.0	34.0	37.0	29.0	39.0
145-149	34.10461921206323	35.0	34.0	37.0	29.0	39.0
150	34.927889923753135	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	5.0
21	168.0
22	1579.0
23	8540.0
24	30649.0
25	75649.0
26	150916.0
27	257098.0
28	377610.0
29	499105.0
30	643112.0
31	837268.0
32	1122784.0
33	1562447.0
34	2340260.0
35	3960365.0
36	9550055.0
37	1387504.0
38	11.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.81338007896207	19.27866922205695	14.578331442749695	33.32961925623128
2	23.69890770614871	26.175434146891618	34.24251923701192	15.88313890994775
3	18.928081391522177	30.09575873004347	29.977872958129108	20.998286920305244
4	22.723058961527286	34.59275930300755	22.635390071310727	20.04879166415444
5	24.583142933829897	35.52144586485166	22.849650554333873	17.045760646984565
6	18.343508909433712	38.759760592116756	23.728734852563882	19.167995645885654
7	19.63457628899437	20.56920154630606	38.680686447493066	21.115535717206498
8	20.775191218938037	23.192108865445277	29.24724573348904	26.785454182127648
9	22.97788563071804	23.28124285385475	29.387488173163867	24.353383342263342
10-14	22.860965431150635	29.15431955279179	25.92469491619143	22.06002009986614
15-19	22.70996605280909	28.3830193096491	27.34844230422396	21.558572333317855
20-24	22.8357368937257	28.41761503893401	27.198290103799906	21.54835796354039
25-29	22.906147376421103	28.431204965444834	27.14458112862077	21.5180665295133
30-34	22.972373036750664	28.17151360894867	27.24944199827909	21.606671356021575
35-39	23.057736972918413	28.150799598704275	27.249842546371262	21.54162088200605
40-44	23.221391400275913	27.902439657538412	27.349254402310496	21.52691453987518
45-49	23.247553061204176	27.714622242621147	27.408421799185774	21.629402896988907
50-54	23.318095970261425	27.664779064812926	27.40804995297768	21.60907501194797
55-59	23.487945495765054	27.56350008487127	27.278821117724977	21.669733301638704
60-64	23.432727416363235	27.520546827216062	27.338241329138324	21.708484427282375
65-69	23.520321289212077	27.388167123676553	27.31713483851852	21.77437674859285
70-74	23.55160652729349	27.390191828542044	27.21772613540667	21.84047550875779
75-79	23.516920272931877	27.437965848602282	27.262867984203197	21.78224589426264
80-84	23.621261955580337	27.49100644563934	27.176157059457385	21.71157453932294
85-89	23.664212743469157	27.38984061395798	27.212513579665103	21.733433062907764
90-94	23.579481287923564	27.33610948419909	27.275212175862396	21.80919705201495
95-99	23.698427509153454	27.459611135643964	27.222893064235933	21.619068290966652
100-104	23.82585896887163	27.46886407712001	27.01894614787702	21.686330806131345
105-109	23.622360470009735	27.604338610492295	27.09868677816154	21.67461414133643
110-114	23.639836009081186	27.58098534365222	27.05516635160576	21.724012295660835
115-119	23.739814884909116	27.599586366724566	27.04873373685373	21.611865011512585
120-124	23.723413226194758	27.478452209665637	27.247142745047036	21.550991819092573
125-129	23.684700371137378	27.554544449405732	27.212708430085343	21.54804674937155
130-134	23.728719505242125	27.471968534728337	27.28288269145105	21.516429268578488
135-139	23.673435552263737	27.49001692224324	27.36576273163509	21.470784793857938
140-144	23.696720782371038	27.62233667439994	27.345264457613123	21.3356780856159
145-149	23.417051161288875	27.700047549109325	27.327933390019027	21.55496789958277
150	23.54223812511971	26.42296390156027	27.794799706508332	22.239998266811686
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	0.5
5	0.0
6	0.0
7	3.0
8	4.5
9	3.0
10	3.5
11	6.5
12	11.0
13	20.0
14	37.5
15	50.5
16	98.0
17	174.0
18	259.5
19	463.0
20	734.0
21	1097.0
22	1702.5
23	2902.5
24	4763.5
25	7597.0
26	12052.5
27	19263.5
28	31598.0
29	48132.5
30	64129.5
31	83021.5
32	107391.5
33	138577.0
34	185794.0
35	254939.0
36	351051.0
37	472092.5
38	614530.0
39	791879.0
40	996363.0
41	1181232.5
42	1349451.5
43	1505073.5
44	1607871.0
45	1648496.5
46	1638443.5
47	1578783.5
48	1472308.5
49	1328048.0
50	1150654.0
51	961035.0
52	781919.5
53	620779.0
54	483249.5
55	367289.0
56	273229.0
57	201343.0
58	145697.5
59	104146.0
60	71179.5
61	46475.5
62	30856.5
63	21073.0
64	14120.5
65	9277.5
66	6545.5
67	4901.5
68	4059.5
69	2872.0
70	1525.0
71	929.0
72	516.5
73	347.0
74	301.0
75	241.0
76	147.5
77	53.0
78	19.5
79	2.0
80	0.5
81	0.0
82	0.0
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	8.769957738012158E-6
5	3.507983095204863E-5
6	1.3154936607018237E-4
7	0.002569597617237562
8	0.11287374106708549
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	8.769957738012158E-7
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	1.7540796472138884E-6
105-109	4.4729767739365434E-5
110-114	0.018767581067455772
115-119	0.0
120-124	0.0
125-129	4.38577409263597E-6
130-134	0.047145444850891095
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62-63	1.0
64-65	2.0
66-67	2.0
68-69	4.0
70-71	1.0
72-73	7.0
74-75	24.0
76-77	24.0
78-79	26.0
80-81	30.0
82-83	57.0
84-85	38.0
86-87	70.0
88-89	116.0
90-91	107.0
92-93	98.0
94-95	101.0
96-97	142.0
98-99	142.0
100-101	161.0
102-103	129.0
104-105	167.0
106-107	144.0
108-109	192.0
110-111	192.0
112-113	195.0
114-115	214.0
116-117	251.0
118-119	259.0
120-121	309.0
122-123	317.0
124-125	395.0
126-127	422.0
128-129	498.0
130-131	591.0
132-133	763.0
134-135	1069.0
136-137	1573.0
138-139	3327.0
140-141	6881.0
142-143	10900.0
144-145	67547.0
146-147	791769.0
148-149	1052586.0
150-151	2.0863284E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.96113539872404
#Duplication Level	Percentage of total
1	29.53688617928662
2	10.71051801525227
3	7.079554791957589
4	5.181445403323867
5	3.9849200467137953
6	3.0803352922496505
7	2.6805786434858576
8	2.1737213888513853
9	1.850293203206264
>10	20.503440431479618
>50	4.960931113859165
>100	6.040422675306176
>500	1.0400340728468287
>1k	0.9183927759711229
>5k	0.1383326749206478
>10k+	0.12019329128930083
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	8.769957738012158E-6	4.384978869006079E-6	0.0	5.52507337494766E-4	0.0
2	0.0	3.069485208304255E-5	4.384978869006079E-6	0.0	0.00485417160798973	0.0
3	3.507983095204863E-5	3.507983095204863E-5	4.384978869006079E-6	0.0	0.006366989317796827	0.0
4	7.454464077310334E-5	3.946480982105471E-5	4.384978869006079E-6	0.0	0.007875422048734919	0.0
5	8.33145985111155E-5	4.823476755906687E-5	4.384978869006079E-6	0.0	0.009625028617468345	0.0
6	1.052394928561459E-4	5.261974642807295E-5	4.384978869006079E-6	4.384978869006079E-6	0.011528109446616983	0.0
7	1.227794083321702E-4	7.454464077310334E-5	4.384978869006079E-6	4.384978869006079E-6	0.013847763268321198	0.0
8	1.4908928154620669E-4	7.454464077310334E-5	4.384978869006079E-6	4.384978869006079E-6	0.019793794614693442	0.0
9	1.6224421815322492E-4	7.892961964210942E-5	4.384978869006079E-6	4.384978869006079E-6	0.02633618308725051	0.0
10-11	1.8197662306375226E-4	8.33145985111155E-5	4.384978869006079E-6	4.384978869006079E-6	0.0334683512176889	0.0
12-13	2.060940068432857E-4	8.33145985111155E-5	4.384978869006079E-6	4.384978869006079E-6	0.03980026070453367	0.0
14-15	2.477513060988435E-4	8.550708794561854E-5	4.384978869006079E-6	4.384978869006079E-6	0.04932224231858037	0.0
16-17	2.894086053544012E-4	9.208455624912766E-5	4.384978869006079E-6	4.384978869006079E-6	0.05541078547819532	2.1924894345030395E-6
18-19	3.5299079895498937E-4	9.866202455263678E-5	4.384978869006079E-6	4.384978869006079E-6	0.06331251740014428	4.384978869006079E-6
20-21	3.924556087760441E-4	1.0962447172515197E-4	4.384978869006079E-6	4.384978869006079E-6	0.0698264035100528	4.384978869006079E-6
22-23	4.384978869006079E-4	1.271643872011763E-4	6.5774683035091185E-6	4.384978869006079E-6	0.0767393226970409	4.384978869006079E-6
24-25	4.933101227631839E-4	1.2935687663567932E-4	8.769957738012158E-6	4.384978869006079E-6	0.08230605337124411	4.384978869006079E-6
26-27	5.437373797567538E-4	1.3154936607018237E-4	8.769957738012158E-6	4.384978869006079E-6	0.08663402751495311	4.384978869006079E-6
28-29	6.577468303509119E-4	1.3374185550468542E-4	8.769957738012158E-6	4.384978869006079E-6	0.09139830705612821	4.384978869006079E-6
30-31	8.638408371941976E-4	1.4031932380819453E-4	8.769957738012158E-6	4.384978869006079E-6	0.09667343663554252	4.384978869006079E-6
32-33	0.0010502024391269558	1.6443670758772795E-4	8.769957738012158E-6	4.384978869006079E-6	0.10229497954560832	4.384978869006079E-6
34-35	0.0011861367840661444	1.775916441947462E-4	8.769957738012158E-6	4.384978869006079E-6	0.1058862772393243	4.384978869006079E-6
36-37	0.0012277940833217023	1.9293907023626748E-4	8.769957738012158E-6	4.384978869006079E-6	0.10947538244360577	4.384978869006079E-6
38-39	0.001265066403708254	1.9293907023626748E-4	8.769957738012158E-6	4.384978869006079E-6	0.11297240309163811	4.384978869006079E-6
40-41	0.0012913762769222903	1.9951653853977658E-4	8.769957738012158E-6	4.384978869006079E-6	0.11606600568372191	4.384978869006079E-6
42-43	0.0013045312135293087	2.1267147514679484E-4	8.769957738012158E-6	4.384978869006079E-6	0.11914645333919868	4.384978869006079E-6
44-45	0.001326456107874339	2.324038800573222E-4	8.769957738012158E-6	4.384978869006079E-6	0.12163931382622864	4.384978869006079E-6
46-47	0.0013549584705228785	2.543287744023526E-4	8.769957738012158E-6	4.384978869006079E-6	0.12432949836236387	4.384978869006079E-6
48-49	0.0013922307909094301	2.76253668747383E-4	8.769957738012158E-6	4.384978869006079E-6	0.12671054188823416	4.384978869006079E-6
50-51	0.0014316956007304849	2.8502362648539516E-4	8.769957738012158E-6	4.384978869006079E-6	0.12894468862199276	6.5774683035091185E-6
52-53	0.0014952777943310729	2.8502362648539516E-4	8.769957738012158E-6	4.384978869006079E-6	0.13115252548253734	8.769957738012158E-6
54-55	0.0015566674984971581	3.003710525269164E-4	8.769957738012158E-6	4.384978869006079E-6	0.13322662048757722	8.769957738012158E-6
56-57	0.0016290196498357583	3.1352598913393463E-4	1.3154936607018237E-5	4.384978869006079E-6	0.1353270253658311	8.769957738012158E-6
58-59	0.0016882168645673404	3.3545088347896506E-4	1.3154936607018237E-5	4.384978869006079E-6	0.13737042551878795	8.769957738012158E-6
60-61	0.0017671464842094501	3.551832883894924E-4	1.3154936607018237E-5	4.384978869006079E-6	0.1390608348727898	8.769957738012158E-6
62-63	0.0018373061461135472	3.639532461275046E-4	1.3154936607018237E-5	4.384978869006079E-6	0.14122262945520977	8.769957738012158E-6
64-65	0.0018636160193275836	3.749156933000198E-4	1.3154936607018237E-5	4.384978869006079E-6	0.14302485577037127	8.769957738012158E-6
66-67	0.00194035314953519	3.924556087760441E-4	1.3154936607018237E-5	4.384978869006079E-6	0.14439735415637017	1.3154936607018237E-5
68-69	0.0019557005755767116	4.3411290803160185E-4	1.3154936607018237E-5	4.384978869006079E-6	0.1457106553276375	1.3154936607018237E-5
70-71	0.001986395427659754	4.4069037633511095E-4	1.3154936607018237E-5	4.384978869006079E-6	0.14698229919964928	1.3154936607018237E-5
72-73	0.002008320322004784	4.691927389836505E-4	1.3154936607018237E-5	4.384978869006079E-6	0.1478636799523195	1.7539915476024316E-5
74-75	0.0020170902797427962	4.7357771785265653E-4	1.3154936607018237E-5	4.384978869006079E-6	0.1489445772435295	1.7539915476024316E-5
76-77	0.002043400152956833	4.889251438941778E-4	1.5347426041521276E-5	4.384978869006079E-6	0.15036750288652195	1.7539915476024316E-5
78-79	0.0020916349205159	5.086575488047052E-4	2.1924894345030395E-5	4.384978869006079E-6	0.15210176202921388	1.7539915476024316E-5
80-81	0.0021025973676884148	5.196199959772204E-4	2.1924894345030395E-5	4.384978869006079E-6	0.15362554218619348	1.7539915476024316E-5
82-83	0.0021223297725989423	5.371599114532446E-4	2.1924894345030395E-5	4.384978869006079E-6	0.155454078374569	1.7539915476024316E-5
84-85	0.002155217114116488	5.437373797567538E-4	2.1924894345030395E-5	4.384978869006079E-6	0.15709406047157728	1.7539915476024316E-5
86-87	0.0021859119661995306	5.546998269292691E-4	2.8502362648539513E-5	4.384978869006079E-6	0.15906949345206453	1.7539915476024316E-5
88-89	0.0021968744133720455	5.634697846672812E-4	3.288734151754559E-5	4.384978869006079E-6	0.16102957900651024	2.1924894345030395E-5
90-91	0.002205644371110058	5.832021895778085E-4	3.507983095204863E-5	4.384978869006079E-6	0.16214555612867232	2.1924894345030395E-5
92-93	0.002210029349979064	5.832021895778085E-4	3.946480982105471E-5	4.384978869006079E-6	0.16318260363119225	2.4117383779533434E-5
94-95	0.002223184286586082	5.897796578813177E-4	3.946480982105471E-5	4.384978869006079E-6	0.16427446336957474	2.6309873214036474E-5
96-97	0.002223184286586082	5.985496156193298E-4	3.946480982105471E-5	4.384978869006079E-6	0.16533782074530873	2.6309873214036474E-5
98-99	0.0022407242020621066	6.007421050538328E-4	3.946480982105471E-5	4.384978869006079E-6	0.16626524377610352	2.6309873214036474E-5
100-101	0.0022451091809311124	6.07319573357342E-4	3.946480982105471E-5	4.384978869006079E-6	0.16708304233517313	2.6309873214036474E-5
102-103	0.002256071628103628	6.182820205298573E-4	3.946480982105471E-5	6.5774683035091185E-6	0.16796880806671238	2.6309873214036474E-5
104-105	0.0022604566069726337	6.314369571368754E-4	3.946480982105471E-5	8.769957738012158E-6	0.1688655362454241	2.6309873214036474E-5
106-107	0.002288958969621173	6.445918937438937E-4	3.946480982105471E-5	8.769957738012158E-6	0.16963290754750018	2.8502362648539513E-5
108-109	0.0023021139062281916	6.533618514819058E-4	4.384978869006079E-5	8.769957738012158E-6	0.17050551834243238	3.069485208304255E-5
110-111	0.002317461332269713	6.730942563924332E-4	4.384978869006079E-5	8.769957738012158E-6	0.17142855639435817	3.069485208304255E-5
112-113	0.002324038800573222	6.840567035649483E-4	4.384978869006079E-5	8.769957738012158E-6	0.17220469765417223	3.069485208304255E-5
114-115	0.002326231290007725	6.928266613029604E-4	4.384978869006079E-5	1.3154936607018237E-5	0.1730224962132419	3.069485208304255E-5
116-117	0.0023284237794422282	7.015966190409726E-4	4.384978869006079E-5	1.3154936607018237E-5	0.17399157654329223	3.288734151754559E-5
118-119	0.002335001247745737	7.081740873444818E-4	4.384978869006079E-5	1.3154936607018237E-5	0.17485103240161742	4.384978869006079E-5
120-121	0.00233719373718024	7.103665767789849E-4	4.384978869006079E-5	1.3154936607018237E-5	0.17581792024223325	4.823476755906687E-5
122-123	0.00233719373718024	7.169440450824939E-4	4.384978869006079E-5	1.3154936607018237E-5	0.17662475635413039	4.823476755906687E-5
124-125	0.0023525411632217613	7.257140028205061E-4	4.823476755906687E-5	1.3154936607018237E-5	0.17759602917361522	4.823476755906687E-5
126-127	0.00236569609982878	7.366764499930213E-4	4.823476755906687E-5	1.3154936607018237E-5	0.1786659640176527	6.13897041660851E-5
128-129	0.002385428504739307	7.432539182965304E-4	4.823476755906687E-5	1.3154936607018237E-5	0.17972274392508314	6.13897041660851E-5
130-131	0.0023963909519118223	7.498313866000396E-4	4.823476755906687E-5	1.3154936607018237E-5	0.18085187598385222	6.13897041660851E-5
132-133	0.0024139308673878463	7.564088549035486E-4	4.823476755906687E-5	1.3154936607018237E-5	0.18178149150408152	6.13897041660851E-5
134-135	0.0024380482511673802	7.586013443380517E-4	5.261974642807295E-5	1.3154936607018237E-5	0.18262779242579968	6.13897041660851E-5
136-137	0.002446818208905392	7.651788126415609E-4	5.261974642807295E-5	1.3154936607018237E-5	0.1835069806890354	7.015966190409726E-5
138-139	0.002446818208905392	7.76141259814076E-4	5.261974642807295E-5	1.3154936607018237E-5	0.1843138168009325	7.454464077310334E-5
>>END_MODULE
