##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.4d.S.1.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24144766
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	67-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.3972335453572	36.0	34.0	38.0	31.0	39.0
2	34.72047217189846	35.0	34.0	38.0	31.0	39.0
3	34.88597694423711	35.0	34.0	38.0	31.0	39.0
4	34.83625262717394	36.0	34.0	38.0	31.0	39.0
5	34.70735914359245	35.0	34.0	37.0	31.0	39.0
6	34.69369369742494	35.0	34.0	37.0	31.0	39.0
7	34.6960765326945	35.0	34.0	37.0	32.0	39.0
8	34.8332912400145	35.0	34.0	37.0	31.0	39.0
9	34.813670797223715	35.0	34.0	37.0	31.0	39.0
10-14	34.7900221604964	35.0	34.0	37.0	31.0	39.0
15-19	34.78842947577127	35.0	34.0	37.0	31.0	39.0
20-24	34.79012402108184	35.0	34.0	37.0	31.0	39.0
25-29	34.786620040136235	35.0	34.0	37.0	31.0	39.0
30-34	34.76107803239841	35.0	34.0	37.0	31.0	39.0
35-39	34.75837963391321	35.0	34.0	37.0	31.0	39.0
40-44	34.684206589535805	35.0	34.0	37.0	31.0	39.0
45-49	34.58049265832604	35.0	34.0	37.0	30.2	39.0
50-54	34.53953664326256	35.0	34.0	37.0	30.0	39.0
55-59	34.53378461402359	35.0	34.0	37.0	30.0	39.0
60-64	34.495192481882	35.0	34.0	37.0	30.0	39.0
65-69	34.47960793604816	35.0	34.0	37.0	30.0	39.0
70-74	34.448223760942604	35.0	34.0	37.0	30.0	39.0
75-79	34.42594539667158	35.0	34.0	37.0	30.0	39.0
80-84	34.39324428141581	35.0	34.0	37.0	30.0	39.0
85-89	34.37527740140454	35.0	34.0	37.0	30.0	39.0
90-94	34.34485785666359	35.0	34.0	37.0	30.0	39.0
95-99	34.30661514340211	35.0	34.0	37.0	30.0	39.0
100-104	34.26790933529679	35.0	34.0	37.0	30.0	39.0
105-109	34.20683110814728	35.0	34.0	37.0	29.2	39.0
110-114	34.17267662571529	35.0	34.0	37.0	29.0	39.0
115-119	34.11871529611406	35.0	34.0	37.0	29.0	39.0
120-124	34.079052225376294	35.0	34.0	37.0	29.0	39.0
125-129	34.01976033303337	35.0	34.0	37.0	29.0	39.0
130-134	33.96515310260569	35.0	34.0	37.0	28.6	39.0
135-139	33.90987352912909	35.0	34.0	37.0	28.0	39.0
140-144	33.88203807438862	35.0	34.0	37.0	28.0	39.0
145-149	33.889474245951284	35.0	34.0	37.0	28.2	39.0
150	34.86424149653117	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	15.0
22	319.0
23	3813.0
24	26100.0
25	100950.0
26	246188.0
27	428636.0
28	610914.0
29	786840.0
30	976745.0
31	1217204.0
32	1543560.0
33	2005191.0
34	2731080.0
35	4151400.0
36	7984945.0
37	1330861.0
38	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.08387424421508	10.430417921631545	15.122730947154345	38.36297688699903
2	22.912472515748362	12.22965121672898	36.476095659111785	28.381780608410878
3	21.707501277192502	16.817834763699196	27.829919997117386	33.64474396199092
4	27.086719248134845	24.644047951615164	21.853259641690297	26.415973158559698
5	26.799843129504264	30.293881193337334	23.90627083138588	19.000004845772523
6	19.90512800239787	35.98954439067751	25.425721035837906	18.679606571086715
7	16.83814166448365	24.235194229795535	39.7012515156002	19.225412590120616
8	19.276416222715532	23.8089402257726	31.09977549144561	25.81486806006626
9	20.086030346324765	22.722692018666095	32.33429845743602	24.856979177573116
10-14	21.077562068341148	28.444157629643822	26.686369680482606	23.791910621532416
15-19	21.28894366896371	27.405634019771686	27.326403406102962	23.97901890516164
20-24	21.2241749901757	27.503767841692422	27.22377707302572	24.048280095106158
25-29	21.177124401363287	27.69422429117096	26.913584026595395	24.21506728087036
30-34	21.179801357431334	27.451389593843718	27.12814842490691	24.24066062381803
35-39	21.48592093412184	27.20084463825611	27.03409337567072	24.279141051951324
40-44	21.636523350355496	27.332763398699043	26.845380819116777	24.18533243182868
45-49	21.879277361879087	27.283291596939417	26.740565508533372	24.096865532648124
50-54	21.848994402209478	27.49236441088395	26.690820660793303	23.967820526113268
55-59	21.761943589333455	27.479209195860836	26.738687750337142	24.020159464468563
60-64	21.97665616539941	27.292338423683372	26.54845192765867	24.182553483258545
65-69	21.81008640916639	27.398072614229058	26.74861701854219	24.043223958062356
70-74	22.171599624043132	27.15759807440185	26.678064786151733	23.992737515403288
75-79	22.05551242623505	27.035746886482155	26.674215763455422	24.234524923827376
80-84	22.27508239107621	27.077074721821166	26.688244324342648	23.95959856275998
85-89	22.153446250579456	27.04646414597856	26.691761351505384	24.108328251936594
90-94	22.14244414240816	26.818450585664838	26.68095874713047	24.358146524796535
95-99	22.34169956261533	26.834047745472873	26.880057370549927	23.944195321361875
100-104	22.27568235817557	26.70667574449767	26.915669245065786	24.10197265226098
105-109	22.380411257289392	26.657683949959548	26.812574390771736	24.14933040197932
110-114	22.363942388063045	26.71832241532715	26.805817005211047	24.111918191398757
115-119	22.452297201714938	26.873211586687475	26.717172092865443	23.957319118732144
120-124	22.404846480555804	26.74286958083123	26.707889672140332	24.14439426647263
125-129	22.778423073585603	26.718490434432525	26.58279786299235	23.92028862898952
130-134	22.553099601519165	27.013273769584018	26.45902395408184	23.974602674814978
135-139	22.620501460215166	26.828011240238876	26.53265012911879	24.01883717042717
140-144	22.576009629056383	26.682612042337812	26.793106326328836	23.948272002276973
145-149	21.980006897448742	26.702164017288194	27.169143387791244	24.14868569747182
150	21.726191132162388	25.356319350006356	27.964532543396214	24.952956974435043
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	0.5
9	2.0
10	3.0
11	3.0
12	6.0
13	14.5
14	27.0
15	57.5
16	104.0
17	205.0
18	471.0
19	859.5
20	1403.5
21	2250.5
22	3815.5
23	6347.5
24	10054.5
25	15527.5
26	22387.0
27	32248.0
28	49532.5
29	73230.5
30	100168.0
31	127545.0
32	154214.5
33	186000.0
34	222713.0
35	271320.0
36	338114.5
37	409509.0
38	485103.5
39	593154.0
40	740726.5
41	903846.5
42	1073646.0
43	1243205.5
44	1390625.0
45	1501497.5
46	1575038.5
47	1592017.5
48	1547423.5
49	1496690.5
50	1402966.0
51	1239621.0
52	1046717.5
53	875827.0
54	729327.5
55	599798.0
56	501594.0
57	408021.5
58	336365.0
59	274513.5
60	207672.5
61	142117.0
62	84098.5
63	49397.0
64	31712.5
65	19386.0
66	12422.0
67	6027.0
68	2481.0
69	1226.5
70	934.0
71	671.0
72	396.5
73	171.5
74	87.0
75	61.5
76	28.0
77	20.0
78	11.0
79	1.5
80	2.0
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.5
87	0.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.2425053115031225E-4
3	4.555852808844783E-5
4	4.555852808844783E-5
5	3.727515934509368E-5
6	9.525874054857272E-5
7	1.6980905923876007E-4
8	1.2010884677863518E-4
9	1.2425053115031225E-4
10-14	1.383322580140143E-4
15-19	2.418743673059412E-4
20-24	1.714657329874309E-4
25-29	2.9985794850942026E-4
30-34	7.993450837336755E-4
35-39	7.50473208147886E-4
40-44	0.0010056009654431936
45-49	8.730670655495274E-4
50-54	5.491873476843801E-4
55-59	3.1890969661913475E-4
60-64	2.526427466723016E-4
65-69	3.387897872158262E-4
70-74	3.3133476894614246E-4
75-79	2.485011384624073E-4
80-84	3.006866291001107E-4
85-89	3.8020766462204953E-4
90-94	3.7026836479525166E-4
95-99	3.2388205997969385E-4
100-104	4.4730692899307335E-4
105-109	3.139448556109625E-4
110-114	2.2448478216005022E-4
115-119	2.4188258207668293E-4
120-124	2.369154849733703E-4
125-129	1.3751319205056783E-4
130-134	1.747966971509621E-4
135-139	1.5161230992538684E-4
140-144	1.5913578395036383E-4
145-149	1.487354717240558E-4
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
66-67	1.0
68-69	0.0
70-71	0.0
72-73	2.0
74-75	2.0
76-77	4.0
78-79	7.0
80-81	8.0
82-83	22.0
84-85	12.0
86-87	16.0
88-89	14.0
90-91	27.0
92-93	29.0
94-95	14.0
96-97	27.0
98-99	44.0
100-101	42.0
102-103	43.0
104-105	48.0
106-107	70.0
108-109	74.0
110-111	88.0
112-113	79.0
114-115	89.0
116-117	110.0
118-119	111.0
120-121	143.0
122-123	178.0
124-125	210.0
126-127	226.0
128-129	285.0
130-131	378.0
132-133	497.0
134-135	652.0
136-137	1172.0
138-139	2654.0
140-141	6244.0
142-143	9962.0
144-145	72764.0
146-147	785762.0
148-149	1484720.0
150-151	2.1777936E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.96375256630666
#Duplication Level	Percentage of total
1	33.425945482623085
2	8.77927549819818
3	5.195833567672832
4	3.6262075766622166
5	2.659810309811234
6	2.2159430134894262
7	1.7959696282441227
8	1.4756033947988139
9	1.3357789652627547
>10	16.450228870244363
>50	5.59158103000546
>100	10.42851437771173
>500	3.1542670909439927
>1k	3.359133152656972
>5k	0.3947838531702821
>10k+	0.11112418850446541
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.141684371677075E-6	8.28336874335415E-6	0.0	0.0	3.271930653624889E-4	2.0708421858385375E-5
2	8.28336874335415E-6	4.141684371677075E-5	4.141684371677075E-6	8.28336874335415E-6	0.002327626616882516	3.31334749734166E-5
3	1.65667374867083E-5	4.97002124601249E-5	4.141684371677075E-6	8.28336874335415E-6	0.004551711124473105	5.384189683180197E-5
4	5.7983581203479045E-5	5.7983581203479045E-5	4.141684371677075E-6	1.2425053115031225E-5	0.006639120047798351	6.212526557515612E-5
5	7.455031869018734E-5	6.212526557515612E-5	4.141684371677075E-6	1.2425053115031225E-5	0.007790508303124578	7.040863431851027E-5
6	9.94004249202498E-5	6.212526557515612E-5	4.141684371677075E-6	1.2425053115031225E-5	0.009654266270379262	7.455031869018734E-5
7	1.2010884677863518E-4	8.28336874335415E-5	4.141684371677075E-6	1.2425053115031225E-5	0.011103855800466238	7.455031869018734E-5
8	2.0708421858385375E-4	8.697537180521857E-5	4.141684371677075E-6	1.2425053115031225E-5	0.014520745407099824	8.697537180521857E-5
9	2.5264274667230154E-4	8.697537180521857E-5	4.141684371677075E-6	1.2425053115031225E-5	0.01745719962661887	9.94004249202498E-5
10-11	3.168388544332962E-4	8.697537180521857E-5	4.141684371677075E-6	1.2425053115031225E-5	0.030853477726808368	1.3046305770782787E-4
12-13	3.58255698150067E-4	8.904621399105711E-5	6.212526557515612E-6	1.2425053115031225E-5	0.03971254059782563	1.4910063738037468E-4
14-15	3.934600153093221E-4	9.525874054857272E-5	8.28336874335415E-6	1.2425053115031225E-5	0.046337164750323116	1.5324232175205178E-4
16-17	4.307351746544158E-4	1.1389632022111955E-4	8.28336874335415E-6	1.2425053115031225E-5	0.050364952801779064	1.7395074361043714E-4
18-19	5.28064757388827E-4	1.3667558426534346E-4	8.28336874335415E-6	1.2425053115031225E-5	0.0534939953445811	1.760215857962757E-4
20-21	8.90462139910571E-4	1.760215857962757E-4	8.28336874335415E-6	1.2425053115031225E-5	0.05610946902529518	1.8016327016795274E-4
22-23	0.0012632137333615078	1.8844663891130688E-4	8.28336874335415E-6	1.4495895300869763E-5	0.05894859366207981	1.9051748109714543E-4
24-25	0.0013771100535826275	1.9051748109714543E-4	8.28336874335415E-6	1.65667374867083E-5	0.061677963663015	1.9258832328298398E-4
26-27	0.0014433770035294606	1.9051748109714543E-4	8.28336874335415E-6	2.4850106230062447E-5	0.06393103996120733	2.0087169202633812E-4
28-29	0.0015344940597063561	1.9051748109714543E-4	8.28336874335415E-6	2.8991790601739523E-5	0.06645953827011618	2.0294253421217667E-4
30-31	0.0017208698564318246	1.9051748109714543E-4	8.28336874335415E-6	3.934600153093221E-5	0.06944983438646703	2.1329674514136937E-4
32-33	0.0018223411235379129	1.9051748109714543E-4	8.28336874335415E-6	4.9700212460124894E-5	0.07232416334041092	2.3607600918559325E-4
34-35	0.0019175998640864858	1.9051748109714543E-4	8.28336874335415E-6	7.24794765043488E-5	0.0749934789179568	2.443593779289474E-4
36-37	0.001975583445289965	1.9051748109714543E-4	8.28336874335415E-6	7.662116087602588E-5	0.07746813533003385	2.5057190448646305E-4
38-39	0.0019942210249625112	1.9051748109714543E-4	8.28336874335415E-6	8.28336874335415E-5	0.08021614291064158	2.547135888581401E-4
40-41	0.0020211419733784123	1.9051748109714543E-4	8.28336874335415E-6	8.697537180521857E-5	0.08266180753211691	2.588552732298172E-4
42-43	0.0020252836577500898	1.9258832328298398E-4	8.28336874335415E-6	9.525874054857272E-5	0.08505570109894625	2.6299695760149424E-4
44-45	0.0020418503952367977	1.9465916546882253E-4	8.28336874335415E-6	9.94004249202498E-5	0.08799008447627946	2.650677997873328E-4
46-47	0.002058417132723506	1.9465916546882253E-4	8.28336874335415E-6	1.0768379366360395E-4	0.09245482022894734	2.650677997873328E-4
48-49	0.0020791255545818915	2.0294253421217667E-4	8.28336874335415E-6	1.1182547803528102E-4	0.0950640813831039	2.692094841590099E-4
50-51	0.0021184715561128236	2.1329674514136937E-4	8.28336874335415E-6	1.1182547803528102E-4	0.09783486822775586	2.692094841590099E-4
52-53	0.002157817557643756	2.1743842951304641E-4	8.28336874335415E-6	1.1182547803528102E-4	0.1004544835928416	2.7128032634484843E-4
54-55	0.0022137302966613965	2.1950927169888496E-4	8.28336874335415E-6	1.1182547803528102E-4	0.10223747871484859	2.7335116853068693E-4
56-57	0.0022758555622365527	2.319343248139162E-4	8.28336874335415E-6	1.1389632022111955E-4	0.10417992868516514	2.795636950882025E-4
58-59	0.0023069181950241303	2.5057190448646305E-4	8.28336874335415E-6	1.2010884677863518E-4	0.10616172465701262	2.816345372740411E-4
60-61	0.0024063186199443807	2.5678443104397864E-4	1.0354210929192687E-5	1.2010884677863518E-4	0.10803169515082482	2.816345372740411E-4
62-63	0.002462231358962021	2.6092611541565574E-4	1.2425053115031225E-5	1.2010884677863518E-4	0.11034896755677814	2.816345372740411E-4
64-65	0.002495364833935438	2.6299695760149424E-4	1.2425053115031225E-5	1.2217968896447373E-4	0.11290645765628873	2.816345372740411E-4
66-67	0.002503648202678792	2.7128032634484843E-4	1.2425053115031225E-5	1.2425053115031225E-4	0.11520302164038368	2.837053794598796E-4
68-69	0.0025264274667230156	2.878470638315567E-4	1.2425053115031225E-5	1.2839221552198932E-4	0.11707092129201004	2.899179060173952E-4
70-71	0.002588552732298172	3.002721169465879E-4	1.2425053115031225E-5	1.3667558426534346E-4	0.11897609610298149	2.940595903890723E-4
72-73	0.0026278987338291043	3.106263278757806E-4	1.2425053115031225E-5	1.3667558426534346E-4	0.12057892795482052	2.982012747607494E-4
74-75	0.002654819682245005	3.2305138099081185E-4	1.2425053115031225E-5	1.3667558426534346E-4	0.1222542392831639	3.106263278757806E-4
76-77	0.002681740630660906	3.251222231766504E-4	1.2425053115031225E-5	1.4081726863702054E-4	0.12357957828210056	3.147680122474577E-4
78-79	0.0026962365259617757	3.271930653624889E-4	1.2425053115031225E-5	1.5117147956621323E-4	0.12487799633262132	3.147680122474577E-4
80-81	0.002739724211864385	3.271930653624889E-4	1.2425053115031225E-5	1.5324232175205178E-4	0.12621161870030134	3.147680122474577E-4
82-83	0.002762503475908609	3.31334749734166E-4	1.2425053115031225E-5	1.6152569049540593E-4	0.12781030886776867	3.147680122474577E-4
84-85	0.0028101328461828954	3.3547643410584304E-4	1.2425053115031225E-5	1.6773821705292155E-4	0.1295684538835456	3.147680122474577E-4
86-87	0.0028805414805014055	3.3961811847752014E-4	1.2425053115031225E-5	1.6980905923876007E-4	0.13112986889166786	3.147680122474577E-4
88-89	0.0029116041132889836	3.416889606633587E-4	1.2425053115031225E-5	1.760215857962757E-4	0.13261259189672825	3.168388544332962E-4
90-91	0.0029261000085898533	3.5618485596422843E-4	1.2425053115031225E-5	1.7809242798211421E-4	0.1346523714497792	3.1890969661913475E-4
92-93	0.0029654460101207853	3.603265403359055E-4	1.2425053115031225E-5	1.8016327016795274E-4	0.13649127931080385	3.2305138099081185E-4
94-95	0.0030213587491384262	3.665390668934211E-4	1.2425053115031225E-5	1.8430495453962984E-4	0.138079615267342	3.2305138099081185E-4
96-97	0.003102121594386129	3.7689327782261376E-4	1.2425053115031225E-5	1.8637579672546836E-4	0.13949607132245556	3.2305138099081185E-4
98-99	0.003180813597447993	3.872474887518065E-4	1.2425053115031225E-5	1.8637579672546836E-4	0.14061432610280838	3.251222231766504E-4
100-101	0.0033071349707841442	3.934600153093221E-4	1.2425053115031225E-5	1.8637579672546836E-4	0.1417719268846921	3.2926390754832745E-4
102-103	0.003348551814500915	3.934600153093221E-4	1.2425053115031225E-5	1.8637579672546836E-4	0.14289846503378828	3.3547643410584304E-4
104-105	0.0033692602363593004	3.934600153093221E-4	1.2425053115031225E-5	1.9051748109714543E-4	0.14396080707512343	3.3961811847752014E-4
106-107	0.0034023937113327168	3.934600153093221E-4	1.2425053115031225E-5	1.9258832328298398E-4	0.14505835343361786	3.3961811847752014E-4
108-109	0.0034541647659786807	3.976016996809992E-4	1.2425053115031225E-5	2.0294253421217667E-4	0.14626772527014759	3.3961811847752014E-4
110-111	0.003528715084668868	4.0588506842435335E-4	1.2425053115031225E-5	2.0915506076969227E-4	0.14737355499738536	3.3961811847752014E-4
112-113	0.003626044667403279	4.0795591061019184E-4	1.2425053115031225E-5	2.1122590295553082E-4	0.14844832209183556	3.416889606633587E-4
114-115	0.0036860990907925965	4.100267527960304E-4	1.65667374867083E-5	2.153675873272079E-4	0.14967633150803783	3.4375980284919724E-4
116-117	0.003733728461066883	4.1623927935354604E-4	1.65667374867083E-5	2.3607600918559328E-4	0.15074281523374466	3.458306450350358E-4
118-119	0.0037896412000845234	4.2659349028273874E-4	1.65667374867083E-5	2.4643022011478595E-4	0.15170368600797374	3.479014872208743E-4
120-121	0.003932529310907383	4.390185433977699E-4	1.65667374867083E-5	2.5678443104397864E-4	0.1526852652040612	3.520431715925514E-4
122-123	0.0041706761622788135	4.390185433977699E-4	1.65667374867083E-5	2.5678443104397864E-4	0.1535840107127151	3.541140137783899E-4
124-125	0.004294926693429127	4.390185433977699E-4	1.65667374867083E-5	2.5678443104397864E-4	0.15443926853546644	3.5618485596422843E-4
126-127	0.0043529102746326055	4.43160227769447E-4	2.0708421858385375E-5	2.5678443104397864E-4	0.1552427553035718	3.6032654033590553E-4
128-129	0.004419177224579439	4.43160227769447E-4	2.0708421858385375E-5	2.6092611541565574E-4	0.1559903293326595	3.6446822470758257E-4
130-131	0.004518577649499689	4.43160227769447E-4	2.0708421858385375E-5	2.692094841590099E-4	0.15672754915081805	3.6446822470758257E-4
132-133	0.004609694705676584	4.43160227769447E-4	2.0708421858385375E-5	2.7128032634484843E-4	0.15750204412832164	3.6446822470758257E-4
134-135	0.004711165972782673	4.452310699552855E-4	2.0708421858385375E-5	2.816345372740411E-4	0.1584297814275773	3.665390668934211E-4
136-137	0.004845770714862177	4.638686496278324E-4	2.0708421858385375E-5	2.816345372740411E-4	0.1593223144096737	3.6860990907925967E-4
138-139	0.004862337452348886	4.762937027428636E-4	2.0708421858385375E-5	2.816345372740411E-4	0.16009888022936317	3.6860990907925967E-4
>>END_MODULE
