##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.4d.S.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24144766
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	62-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.94442965402936	37.0	35.0	38.0	33.0	39.0
2	35.280998623055616	35.0	34.0	37.0	33.0	39.0
3	35.42788308654555	35.0	34.0	37.0	33.0	39.0
4	35.48154312201659	36.0	35.0	37.0	33.0	39.0
5	35.402660767141	36.0	35.0	37.0	33.0	39.0
6	35.358417803676375	35.0	34.0	37.0	33.0	39.0
7	35.40075795309012	35.0	34.0	37.0	33.0	39.0
8	35.49228408343241	36.0	34.0	38.0	33.0	39.0
9	35.47402608913253	36.0	34.0	38.0	33.0	39.0
10-14	35.44904219821389	35.8	34.4	37.8	33.0	39.0
15-19	35.42726098070281	36.0	34.0	38.0	33.0	39.0
20-24	35.41336915006755	36.0	34.0	38.0	33.0	39.0
25-29	35.396146800511545	36.0	34.0	38.0	33.0	39.0
30-34	35.378098135223176	36.0	34.0	38.0	32.8	39.0
35-39	35.3476279621016	36.0	34.0	38.0	32.4	39.0
40-44	35.33188509675348	36.0	34.0	38.0	32.0	39.0
45-49	35.30137515517856	36.0	34.0	38.0	32.0	39.0
50-54	35.27915930930952	36.0	34.0	38.0	32.0	39.0
55-59	35.25321188865529	36.0	34.0	38.0	32.0	39.0
60-64	35.2088666124428	36.0	34.0	38.0	32.0	39.0
65-69	35.181159889579774	36.0	34.0	38.0	32.0	39.0
70-74	35.144905136843605	36.0	34.0	38.0	32.0	39.0
75-79	35.095263272072714	35.4	34.0	37.8	32.0	39.0
80-84	35.05115553228046	35.2	34.0	37.6	32.0	39.0
85-89	34.99946465501464	35.0	34.0	37.2	31.8	39.0
90-94	34.944156481566516	35.0	34.0	37.0	31.0	39.0
95-99	34.87571333159009	35.0	34.0	37.0	31.0	39.0
100-104	34.82409190370852	35.0	34.0	37.0	31.0	39.0
105-109	34.76378009270066	35.0	34.0	37.0	31.0	39.0
110-114	34.68556297916443	35.0	34.0	37.0	30.4	39.0
115-119	34.60679790767459	35.0	34.0	37.0	30.0	39.0
120-124	34.53026066629228	35.0	34.0	37.0	30.0	39.0
125-129	34.446565181429364	35.0	34.0	37.0	30.0	39.0
130-134	34.33542502745902	35.0	34.0	37.0	29.8	39.0
135-139	34.27286503630145	35.0	34.0	37.0	29.0	39.0
140-144	34.14660340395311	35.0	34.0	37.0	29.0	39.0
145-149	34.12818282734733	35.0	34.0	37.0	29.0	39.0
150	34.95790693272933	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	170.0
22	1784.0
23	9786.0
24	34400.0
25	84089.0
26	162472.0
27	271091.0
28	394016.0
29	516737.0
30	664028.0
31	865854.0
32	1156843.0
33	1607968.0
34	2404324.0
35	4037837.0
36	9917607.0
37	2015751.0
38	2.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.68780215140623	19.15563812049369	15.251350955316775	31.9052087727833
2	23.7893256037354	26.467077792346384	32.738904986695665	17.004691617222548
3	19.572320560075006	30.13335064005176	29.40785178866509	20.886477011208143
4	23.01754757117961	34.877840605288945	21.66347770775662	20.441134115774823
5	24.869982173362125	35.442675236529524	21.874098924793888	17.813243665314463
6	18.841806128914236	39.13598914149758	22.259602764425217	19.76260196516297
7	20.297413526393257	20.763965987493936	37.056490835322236	21.882129650790567
8	21.43074403785897	23.50605510113455	28.225554142873033	26.837646718133445
9	23.431798842034752	23.65214059229234	27.788465624392465	25.12759494128044
10-14	23.022119162389064	28.835671466022905	25.331562956543046	22.81064641504498
15-19	22.974910587246942	27.76138149361232	26.900390751353733	22.363317167787006
20-24	22.903224657468208	27.717011628938543	26.810821028458093	22.568942685135156
25-29	22.970119486765785	27.367349097522837	26.78266502976256	22.879866385948823
30-34	22.896883928358502	27.05967270140181	26.97524280811206	23.06820056212763
35-39	23.176730724994393	26.791206839610705	27.055568068044227	22.97649436735067
40-44	23.36846668963369	26.40803725329125	27.084350289416765	23.1391457676583
45-49	23.258910854634085	26.27881007419993	27.124623199910076	23.337655871255908
50-54	23.51388536960764	26.30515201514067	26.809708572035863	23.371254043215824
55-59	23.538135759940683	26.332729834697922	26.804266398771475	23.324868006589917
60-64	23.25192835999203	26.305968362765963	26.902478605963353	23.53962467127865
65-69	23.492603049764632	26.239154914662734	26.86136689790302	23.406875137669616
70-74	23.63370746012095	26.200456943942612	26.59931456751934	23.566521028417103
75-79	23.447543788643145	26.333801981948685	26.83956923756262	23.379084991845552
80-84	23.7430113269852	26.317570881482112	26.55370042842175	23.38571736311094
85-89	23.49152491801973	26.39069206027137	26.756644744930963	23.361138276777936
90-94	23.525386646522225	26.142866569411353	26.778389447202837	23.55335733686358
95-99	23.559943281314144	26.290833858782463	26.76918115752109	23.380041702382297
100-104	23.760913696795612	26.134758978573817	26.720202224948252	23.38412509968232
105-109	23.588515013185386	26.340325486858806	26.588432565962272	23.48272693399354
110-114	23.66818091690813	26.423179904300643	26.48422877082969	23.42441040796153
115-119	23.82971343673173	26.50740571304076	26.64244754782768	23.02043330239983
120-124	23.790620350779307	26.650372771864784	26.761194209009965	22.797812668345948
125-129	23.753399779620278	26.72802815983941	26.591512158831463	22.927059901708844
130-134	23.89963953783673	26.74170140962736	26.6130226517138	22.745636400822107
135-139	23.654038827685362	26.75352789626997	26.900361621061027	22.692071654983643
140-144	23.70868976565669	26.851876171139516	26.687053580785182	22.752380482418612
145-149	23.537637924000986	27.06721348327632	26.517252093437587	22.877896499285107
150	23.732189237891355	25.914895899438743	26.791641037180604	23.561273825489295
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	2.0
11	4.0
12	4.0
13	6.5
14	17.5
15	25.0
16	51.0
17	100.0
18	180.0
19	330.5
20	504.0
21	697.5
22	1067.0
23	1844.5
24	3024.0
25	4723.0
26	7242.0
27	11412.5
28	17514.0
29	24729.5
30	34910.0
31	50639.0
32	72614.5
33	99912.0
34	135540.5
35	192015.0
36	273007.0
37	368817.5
38	488019.5
39	635431.0
40	809742.5
41	997739.0
42	1188422.5
43	1382105.0
44	1532831.5
45	1623971.5
46	1648857.5
47	1612859.0
48	1561143.0
49	1502064.5
50	1387803.5
51	1222508.0
52	1033778.0
53	862857.5
54	732682.0
55	627806.5
56	510906.0
57	392049.5
58	318744.0
59	258465.0
60	190328.5
61	126484.0
62	79890.0
63	49547.0
64	30348.5
65	15186.5
66	8302.0
67	4888.5
68	3313.0
69	2150.5
70	916.0
71	582.0
72	359.5
73	265.5
74	236.5
75	149.0
76	89.5
77	36.0
78	14.0
79	3.0
80	1.5
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	8.28336874335415E-7
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	8.283370184252553E-7
70-74	0.0
75-79	0.0
80-84	8.283387063385463E-7
85-89	0.0
90-94	8.283439485196163E-7
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	4.970634609122094E-6
130-134	0.05346631653540721
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62-63	1.0
64-65	3.0
66-67	1.0
68-69	1.0
70-71	4.0
72-73	5.0
74-75	5.0
76-77	6.0
78-79	12.0
80-81	12.0
82-83	21.0
84-85	29.0
86-87	28.0
88-89	32.0
90-91	43.0
92-93	49.0
94-95	55.0
96-97	68.0
98-99	79.0
100-101	111.0
102-103	107.0
104-105	110.0
106-107	112.0
108-109	117.0
110-111	144.0
112-113	167.0
114-115	173.0
116-117	161.0
118-119	231.0
120-121	242.0
122-123	302.0
124-125	346.0
126-127	395.0
128-129	527.0
130-131	588.0
132-133	780.0
134-135	1013.0
136-137	1784.0
138-139	4479.0
140-141	8376.0
142-143	10677.0
144-145	64455.0
146-147	801425.0
148-149	1098522.0
150-151	2.2148968E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.18016905657005
#Duplication Level	Percentage of total
1	25.63812972047888
2	8.39308714596726
3	5.294762250433028
4	3.7802217056261895
5	2.8279713213733326
6	2.2374406223439074
7	1.8136457061299682
8	1.5910439726535437
9	1.352805865051721
>10	17.456030292301193
>50	6.238303846353007
>100	12.409098401805192
>500	3.752744991750285
>1k	5.418254071256811
>5k	0.9864044096417824
>10k+	0.8100556768340113
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	34557	0.14312418683204467	No Hit
GAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCCG	26126	0.10820564589443527	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.5264274667230154E-4	4.141684371677075E-6	0.0	0.0	0.001768499226706111	0.0
2	0.001433022792600268	4.141684371677075E-6	0.0	0.0	0.00892118813659242	0.0
3	0.0021288257670420163	4.141684371677075E-6	0.0	0.0	0.01313742282695968	0.0
4	0.005214380623941437	8.28336874335415E-6	0.0	4.141684371677075E-6	0.019660575712351074	0.0
5	0.005404898105038583	8.28336874335415E-6	0.0	4.141684371677075E-6	0.023396375015603797	0.0
6	0.006407185722984435	8.28336874335415E-6	0.0	4.141684371677075E-6	0.031228300162445145	0.0
7	0.008386910852646076	1.65667374867083E-5	0.0	4.141684371677075E-6	0.04091569990779782	0.0
8	0.015195839959683188	1.65667374867083E-5	0.0	4.141684371677075E-6	0.05532461983686236	0.0
9	0.015796384193576363	1.65667374867083E-5	0.0	4.141684371677075E-6	0.06548417160058623	0.0
10-11	0.016490116325832273	1.863757967254684E-5	0.0	4.141684371677075E-6	0.11103648716247654	0.0
12-13	0.017225265301804953	2.0708421858385375E-5	0.0	4.141684371677075E-6	0.13108638120576527	0.0
14-15	0.02027354499935928	3.106263278757806E-5	4.141684371677075E-6	4.141684371677075E-6	0.1418671856252407	0.0
16-17	0.023754630713753864	3.31334749734166E-5	4.141684371677075E-6	4.141684371677075E-6	0.1497446693001705	0.0
18-19	0.030578055716091845	3.5204317159255134E-5	4.141684371677075E-6	4.141684371677075E-6	0.15685179968196833	0.0
20-21	0.03612170024758161	3.727515934509367E-5	4.141684371677075E-6	4.141684371677075E-6	0.16182389177016665	2.0708421858385374E-6
22-23	0.038461751917579154	4.97002124601249E-5	4.141684371677075E-6	4.141684371677075E-6	0.16717908966274514	4.141684371677075E-6
24-25	0.044195913930166066	4.97002124601249E-5	4.141684371677075E-6	4.141684371677075E-6	0.17179706773716508	4.141684371677075E-6
26-27	0.049147297596506004	4.97002124601249E-5	4.141684371677075E-6	4.141684371677075E-6	0.1756343383075239	4.141684371677075E-6
28-29	0.06145431270694444	5.384189683180197E-5	4.141684371677075E-6	4.141684371677075E-6	0.17911542402191846	4.141684371677075E-6
30-31	0.0857142289140429	5.384189683180197E-5	4.141684371677075E-6	4.141684371677075E-6	0.18329438355294064	4.141684371677075E-6
32-33	0.10574341453547323	5.7983581203479045E-5	4.141684371677075E-6	4.141684371677075E-6	0.18648348051913197	4.141684371677075E-6
34-35	0.11966568655086572	6.62669499468332E-5	4.141684371677075E-6	4.141684371677075E-6	0.189419934738651	4.141684371677075E-6
36-37	0.12185456674129706	6.62669499468332E-5	4.141684371677075E-6	4.141684371677075E-6	0.19149284776667538	4.141684371677075E-6
38-39	0.12390884218964889	6.833779213267173E-5	4.141684371677075E-6	4.141684371677075E-6	0.19319922172780635	4.141684371677075E-6
40-41	0.12532943992913412	7.040863431851027E-5	4.141684371677075E-6	4.141684371677075E-6	0.19500499611385758	4.141684371677075E-6
42-43	0.1256027910976648	7.040863431851027E-5	4.141684371677075E-6	4.141684371677075E-6	0.19693295018887325	4.141684371677075E-6
44-45	0.12649325323757538	7.455031869018734E-5	4.141684371677075E-6	4.141684371677075E-6	0.19839910645644693	4.141684371677075E-6
46-47	0.1279076384505031	8.904621399105711E-5	4.141684371677075E-6	4.141684371677075E-6	0.19993567135833912	4.141684371677075E-6
48-49	0.1290838768120594	9.318789836273418E-5	4.141684371677075E-6	4.141684371677075E-6	0.20115746824798386	4.141684371677075E-6
50-51	0.13016485643306713	9.94004249202498E-5	4.141684371677075E-6	4.141684371677075E-6	0.20245174461413293	4.141684371677075E-6
52-53	0.13135559068992428	9.94004249202498E-5	4.141684371677075E-6	4.141684371677075E-6	0.2037605168755829	6.212526557515612E-6
54-55	0.13196855997693246	1.2010884677863518E-4	6.212526557515612E-6	4.141684371677075E-6	0.20497817208085595	8.28336874335415E-6
56-57	0.1327554800075511	1.2839221552198932E-4	8.28336874335415E-6	4.141684371677075E-6	0.20617097717989896	8.28336874335415E-6
58-59	0.13292943075116156	1.3460474207950492E-4	1.0354210929192687E-5	4.141684371677075E-6	0.20723124837904827	8.28336874335415E-6
60-61	0.13325248213215235	1.4081726863702054E-4	1.2425053115031225E-5	4.141684371677075E-6	0.208107214623658	8.28336874335415E-6
62-63	0.13355275424909896	1.4910063738037468E-4	1.2425053115031225E-5	4.141684371677075E-6	0.20905773118695786	8.28336874335415E-6
64-65	0.13358588772407237	1.553131639378903E-4	1.2425053115031225E-5	4.141684371677075E-6	0.21011593154392136	8.28336874335415E-6
66-67	0.13368321730680677	1.760215857962757E-4	1.2425053115031225E-5	8.28336874335415E-6	0.21084693883552236	1.0354210929192687E-5
68-69	0.1337536259411253	1.8223411235379129E-4	1.2425053115031225E-5	8.28336874335415E-6	0.21171048002701703	1.2425053115031225E-5
70-71	0.13394000173785076	1.8223411235379129E-4	1.2425053115031225E-5	8.28336874335415E-6	0.21257195037632587	1.65667374867083E-5
72-73	0.1339959144768684	1.9465916546882253E-4	1.2425053115031225E-5	8.28336874335415E-6	0.21318699050551992	1.65667374867083E-5
74-75	0.13401869374091263	1.9673000765466108E-4	1.2425053115031225E-5	8.28336874335415E-6	0.2139863355892536	1.65667374867083E-5
76-77	0.13407667732211612	2.0294253421217667E-4	1.2425053115031225E-5	8.28336874335415E-6	0.21486023099167745	1.65667374867083E-5
78-79	0.13415122764080628	2.1122590295553082E-4	1.2425053115031225E-5	8.28336874335415E-6	0.21603854019541957	1.65667374867083E-5
80-81	0.13420299869545227	2.153675873272079E-4	1.2425053115031225E-5	8.28336874335415E-6	0.21696627749467523	1.65667374867083E-5
82-83	0.13422991964386816	2.1950927169888496E-4	1.2425053115031225E-5	8.28336874335415E-6	0.2179250774267185	2.0708421858385375E-5
84-85	0.13428169069851414	2.2986348262807766E-4	1.2425053115031225E-5	8.28336874335415E-6	0.21887559399001838	2.0708421858385375E-5
86-87	0.13435209933283265	2.3193432481391618E-4	1.4495895300869763E-5	1.0354210929192687E-5	0.21988202329233592	2.485010623006245E-5
88-89	0.1343873036499919	2.3400516699975473E-4	1.863757967254684E-5	1.2425053115031225E-5	0.22081804396033491	2.485010623006245E-5
90-91	0.13442043712496532	2.443593779289474E-4	2.0708421858385375E-5	1.2425053115031225E-5	0.2214351549317148	2.485010623006245E-5
92-93	0.13444942891556705	2.485010623006245E-4	2.0708421858385375E-5	1.2425053115031225E-5	0.2219880697953337	2.485010623006245E-5
94-95	0.13449705828584133	2.485010623006245E-4	2.0708421858385375E-5	1.2425053115031225E-5	0.22269629782289047	2.485010623006245E-5
96-97	0.13450327081239885	2.5678443104397864E-4	2.0708421858385375E-5	1.2425053115031225E-5	0.2232554252130669	2.485010623006245E-5
98-99	0.13452397923425724	2.650677997873328E-4	2.277926404422391E-5	1.2425053115031225E-5	0.22390359881723434	2.8991790601739523E-5
100-101	0.1345550418670448	2.6713864197317133E-4	2.485010623006245E-5	1.2425053115031225E-5	0.22448343462926912	3.31334749734166E-5
102-103	0.13456746692015983	2.692094841590099E-4	2.6920948415900986E-5	1.2425053115031225E-5	0.2250508453881889	3.727515934509367E-5
104-105	0.13460060039513327	2.7128032634484843E-4	2.8991790601739523E-5	1.2425053115031225E-5	0.2256327520424095	4.3487685902609285E-5
106-107	0.13463166302792084	2.7335116853068693E-4	3.31334749734166E-5	1.2425053115031225E-5	0.2262374379606744	4.97002124601249E-5
108-109	0.13466479650289426	2.7749285290236403E-4	3.31334749734166E-5	1.2425053115031225E-5	0.22680070703522245	4.97002124601249E-5
110-111	0.13472070924191187	2.7749285290236403E-4	3.31334749734166E-5	1.2425053115031225E-5	0.22740125126911565	4.97002124601249E-5
112-113	0.1347372759793986	2.7749285290236403E-4	3.31334749734166E-5	1.2425053115031225E-5	0.22797694539677874	4.97002124601249E-5
114-115	0.13475177187469947	2.7749285290236403E-4	3.31334749734166E-5	1.2425053115031225E-5	0.2286230481587604	4.97002124601249E-5
116-117	0.13476833861218618	2.7749285290236403E-4	3.31334749734166E-5	1.2425053115031225E-5	0.22914697123177752	4.97002124601249E-5
118-119	0.13477248029655786	2.899179060173952E-4	3.727515934509367E-5	1.2425053115031225E-5	0.22966261093605134	5.591273901764051E-5
120-121	0.13477869282311536	2.899179060173952E-4	3.727515934509367E-5	1.65667374867083E-5	0.23030871369803296	5.7983581203479045E-5
122-123	0.13479940124497375	2.899179060173952E-4	3.727515934509367E-5	1.65667374867083E-5	0.23078707824296163	6.212526557515612E-5
124-125	0.13483046387776132	2.9198874820323377E-4	3.727515934509367E-5	1.65667374867083E-5	0.23130893047379297	6.833779213267173E-5
126-127	0.13486566819492057	2.9820127476074936E-4	3.727515934509367E-5	1.65667374867083E-5	0.2319405373404737	7.869200306186443E-5
128-129	0.13487395156366394	3.04413801318265E-4	3.727515934509367E-5	1.65667374867083E-5	0.23264876536803047	8.904621399105711E-5
130-131	0.13488016409022147	3.106263278757806E-4	3.934600153093221E-5	2.0708421858385375E-5	0.23331350570968468	9.111705617689564E-5
132-133	0.13490294335426567	3.1269717006161916E-4	4.555852808844782E-5	2.0708421858385375E-5	0.2339016248904628	9.111705617689564E-5
134-135	0.13492158093393825	3.209805388049733E-4	4.555852808844782E-5	2.0708421858385375E-5	0.23444832722752418	9.111705617689564E-5
136-137	0.13492986430268158	3.2305138099081185E-4	4.555852808844782E-5	2.0708421858385375E-5	0.23499295872239973	9.111705617689564E-5
138-139	0.13493193514486743	3.2305138099081185E-4	4.555852808844782E-5	2.0708421858385375E-5	0.2356038571672221	9.525874054857272E-5
>>END_MODULE
