##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.4d.S.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24091839
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	63-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.12208096691996	37.0	35.0	38.0	33.0	39.0
2	35.50035549382511	35.0	34.0	38.0	33.0	39.0
3	35.605317966801955	36.0	35.0	38.0	33.0	39.0
4	35.67512911737456	36.0	35.0	38.0	33.0	39.0
5	35.598993335461024	36.0	35.0	38.0	33.0	39.0
6	35.538195901109916	36.0	35.0	37.0	33.0	39.0
7	35.563836284976006	35.0	34.0	38.0	33.0	39.0
8	35.62805529291475	36.0	35.0	38.0	33.0	39.0
9	35.64893323419603	36.0	35.0	38.0	33.0	39.0
10-14	35.6395791039447	36.0	35.0	37.8	33.0	39.0
15-19	35.61468386867436	36.0	35.0	38.0	33.0	39.0
20-24	35.600707592309575	36.0	35.0	38.0	33.0	39.0
25-29	35.58514140825862	36.0	35.0	38.0	33.0	39.0
30-34	35.565508079312664	36.0	35.0	38.0	33.0	39.0
35-39	35.539012609207624	36.0	35.0	38.0	33.0	39.0
40-44	35.52095719218446	36.0	35.0	38.0	33.0	39.0
45-49	35.49260841399446	36.0	35.0	38.0	33.0	39.0
50-54	35.472724145300816	36.0	35.0	38.0	33.0	39.0
55-59	35.44778477060219	36.0	34.8	38.0	33.0	39.0
60-64	35.40182921195732	36.0	34.0	38.0	33.0	39.0
65-69	35.375571165488665	36.0	34.0	38.0	32.8	39.0
70-74	35.34690895952935	36.0	34.0	38.0	32.0	39.0
75-79	35.296624051747656	36.0	34.0	38.0	32.0	39.0
80-84	35.25333185837847	36.0	34.0	38.0	32.0	39.0
85-89	35.20308125508772	36.0	34.0	38.0	32.0	39.0
90-94	35.14998260684753	36.0	34.0	38.0	32.0	39.0
95-99	35.08366289093903	36.0	34.0	38.0	32.0	39.0
100-104	35.03514035240648	36.0	34.0	38.0	32.0	39.0
105-109	34.974109515026775	35.0	34.0	37.4	31.4	39.0
110-114	34.894860600603465	35.0	34.0	37.0	31.0	39.0
115-119	34.83067605406879	35.0	34.0	37.0	31.0	39.0
120-124	34.75783091425122	35.0	34.0	37.0	31.0	39.0
125-129	34.677224976328944	35.0	34.0	37.0	30.6	39.0
130-134	34.57580766748625	35.0	34.0	37.0	30.0	39.0
135-139	34.517755010105326	35.0	34.0	37.0	30.0	39.0
140-144	34.399267843217146	35.0	34.0	37.0	30.0	39.0
145-149	34.38323354964295	35.0	34.0	37.0	30.0	39.0
150	35.1176517945482	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	65.0
22	711.0
23	4401.0
24	16871.0
25	47048.0
26	106340.0
27	206407.0
28	326168.0
29	443624.0
30	582950.0
31	772263.0
32	1050192.0
33	1497884.0
34	2291689.0
35	4004113.0
36	1.0608545E7
37	2132562.0
38	5.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.087592898159414	19.66849438102255	14.824244010596285	31.41966871022175
2	25.093916657835873	25.886795109331423	31.263661524551946	17.755626708280758
3	20.85116042822634	29.25982113694185	28.90191985759161	20.9870985772402
4	24.492105956557232	33.032156619362674	21.77718211264596	20.69855531143413
5	26.337449140438967	34.19307838440846	21.672996889568218	17.79647558558436
6	20.35951045120424	38.212983271456864	21.953051527827895	19.474454749511004
7	21.554719216658135	21.01534482818737	36.12126878960672	21.30866716554778
8	22.628456354531103	23.589792391209944	27.74006077956251	26.041690474696445
9	24.196206026447378	23.025245187799904	27.844101066755428	24.93444771899729
10-14	23.8766853788123	28.54256497397314	24.917588067893032	22.663161579321528
15-19	23.84001154913911	27.61283188053847	26.363842129278716	22.18331444104371
20-24	23.71145266245553	27.67166010033522	26.326800540216126	22.290086696993118
25-29	23.816929874054033	27.376926269513923	26.30990602253319	22.496237833898856
30-34	23.563673159197187	27.206499263090713	26.55972671907695	22.67010085863516
35-39	23.843610278152696	27.053586071200293	26.585191773861677	22.517611876785327
40-44	23.950776028347192	26.85941409454048	26.654922440748503	22.534887436363825
45-49	23.87516287154335	26.768173239079008	26.74814404994156	22.608519839436084
50-54	23.997392644040165	26.71284911043943	26.55452910838396	22.73522913713644
55-59	24.063044751378257	26.725872607732438	26.59907697374202	22.612005667147287
60-64	23.77295667444031	26.769113198615624	26.6834418633085	22.77448826363557
65-69	23.954700238781307	26.770215051608602	26.70642746707104	22.568657242539043
70-74	24.080471602237903	26.74091563330105	26.448843981452963	22.72976878300808
75-79	23.85994935143037	26.819019816837713	26.735977507647867	22.58505332408405
80-84	24.119917654587773	26.826829530035578	26.471501775870877	22.581751039505775
85-89	23.868676896788696	26.834592695673248	26.780179031134093	22.516551376403964
90-94	23.89520686595939	26.622630878972082	26.793352074410958	22.688810180657573
95-99	23.792790404384732	26.85992500203494	26.84304109978309	22.504243493797233
100-104	24.04394194821017	26.69221040080801	26.782485619643204	22.481362031338616
105-109	23.82790726000847	26.76792939320467	26.9001999133826	22.503963433404255
110-114	23.865923269523666	26.874784531206625	26.798045900907226	22.461246298362482
115-119	23.97136386772753	26.880124733738974	26.939949381545343	22.208562016988154
120-124	23.894131801400395	26.9586474510552	27.095651491555277	22.051569255989122
125-129	23.840429214404676	27.046437958368298	27.01745231058527	22.095680516641753
130-134	23.980932680202756	26.96959249434665	27.088460679635656	21.961014145814932
135-139	23.747699257859722	27.042335515313138	27.310229661832224	21.899735564994913
140-144	23.736435287317462	27.159734309449213	27.13931566967855	21.96451473355478
145-149	23.549404563223533	27.436380590971172	26.93836635598138	22.075848489823912
150	23.60353101772862	26.412682949395773	27.24079680735602	22.742989225519587
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	0.0
6	0.5
7	0.5
8	0.0
9	1.0
10	1.5
11	2.5
12	6.5
13	11.5
14	16.5
15	30.0
16	49.5
17	81.0
18	159.0
19	296.0
20	487.5
21	721.5
22	1156.5
23	1917.5
24	3033.0
25	4943.5
26	7624.0
27	11614.0
28	17549.5
29	25294.0
30	35714.0
31	50743.5
32	72013.0
33	98611.5
34	131632.0
35	181555.5
36	255162.0
37	348315.5
38	462859.0
39	603266.0
40	772975.5
41	964900.0
42	1164710.5
43	1362049.0
44	1528670.5
45	1631352.5
46	1668006.5
47	1652454.0
48	1610108.5
49	1559358.5
50	1446540.5
51	1273624.0
52	1065281.5
53	867241.0
54	719433.0
55	592804.0
56	479818.0
57	386414.0
58	315087.5
59	249130.0
60	180047.0
61	115873.0
62	69273.5
63	41275.0
64	25744.0
65	14796.5
66	8624.0
67	4605.0
68	2441.0
69	1427.0
70	845.0
71	645.5
72	467.0
73	363.5
74	279.5
75	182.5
76	113.5
77	37.5
78	8.5
79	2.0
80	0.5
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	1.2452349527987464E-5
5	2.9055482231970748E-5
6	9.131722987190808E-5
7	0.002469715989717514
8	0.11551214500478772
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	8.30158475260547E-7
85-89	0.0
90-94	0.0
95-99	0.0
100-104	8.30170384169647E-7
105-109	3.9848503957653795E-5
110-114	0.019042778516886676
115-119	0.0
120-124	0.0
125-129	3.320900159876436E-6
130-134	0.047176799568824875
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62-63	1.0
64-65	2.0
66-67	1.0
68-69	4.0
70-71	3.0
72-73	4.0
74-75	5.0
76-77	7.0
78-79	11.0
80-81	15.0
82-83	16.0
84-85	13.0
86-87	27.0
88-89	20.0
90-91	30.0
92-93	40.0
94-95	30.0
96-97	44.0
98-99	58.0
100-101	65.0
102-103	77.0
104-105	83.0
106-107	82.0
108-109	91.0
110-111	80.0
112-113	106.0
114-115	117.0
116-117	134.0
118-119	119.0
120-121	162.0
122-123	196.0
124-125	204.0
126-127	264.0
128-129	329.0
130-131	403.0
132-133	546.0
134-135	730.0
136-137	1399.0
138-139	3914.0
140-141	8023.0
142-143	9115.0
144-145	58817.0
146-147	782645.0
148-149	1002225.0
150-151	2.2221582E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.05479519395208
#Duplication Level	Percentage of total
1	27.483785365801065
2	8.548590915354195
3	5.25916792950812
4	3.6468545422240326
5	2.802281372623
6	2.2950930843442956
7	1.8096968009589587
8	1.563238922638565
9	1.3400012567336816
>10	17.506270152348115
>50	6.260126571345941
>100	12.729406406435126
>500	3.5841605702669663
>1k	4.473729224331625
>5k	0.47220531757872863
>10k+	0.22539156750769423
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.7810247279172007E-4	4.150783175995822E-6	0.0	0.0	0.0012078779042147842	0.0
2	0.0012244810369187674	4.150783175995822E-6	0.0	0.0	0.005694874517466268	0.0
3	0.0018720032123741156	4.150783175995822E-6	0.0	0.0	0.00795705134838399	0.0
4	0.0044952981796034745	4.150783175995822E-6	0.0	0.0	0.011074289513556853	0.0
5	0.004636424807587333	4.150783175995822E-6	0.0	0.0	0.012776110615715139	0.0
6	0.005553747889482409	4.150783175995822E-6	0.0	0.0	0.016673696017975217	0.0
7	0.007214061159880738	8.301566351991643E-6	0.0	0.0	0.020442607141779423	0.0
8	0.012232358019659686	1.2452349527987464E-5	0.0	0.0	0.027694025350244122	0.0
9	0.012693094952195222	1.2452349527987464E-5	0.0	0.0	0.03292816293517485	0.0
10-11	0.013247224506190664	1.4527741115985375E-5	0.0	0.0	0.05495221846700868	0.0
12-13	0.013755695445250153	1.6603132703983287E-5	0.0	2.075391587997911E-6	0.06558237418073398	0.0
14-15	0.015741845194964153	2.075391587997911E-5	0.0	4.150783175995822E-6	0.07196212792223956	0.0
16-17	0.018124394737985758	2.490469905597493E-5	0.0	4.150783175995822E-6	0.07599046299454351	0.0
18-19	0.022513847946601336	2.698009064397284E-5	4.150783175995822E-6	4.150783175995822E-6	0.08069537572453478	0.0
20-21	0.02647577048808935	2.905548223197075E-5	4.150783175995822E-6	4.150783175995822E-6	0.08383336780558762	0.0
22-23	0.028181742373423628	4.565861493595404E-5	4.150783175995822E-6	4.150783175995822E-6	0.08728681940801614	0.0
24-25	0.031685003373964106	4.980939811194986E-5	4.150783175995822E-6	4.150783175995822E-6	0.09072989405250467	0.0
26-27	0.03463413482050914	6.018635605193941E-5	4.150783175995822E-6	4.150783175995822E-6	0.09367487471587371	0.0
28-29	0.04144557001231828	6.226174763993732E-5	4.150783175995822E-6	4.150783175995822E-6	0.0960678012168353	0.0
30-31	0.05582180754237981	6.848792240393106E-5	4.150783175995822E-6	4.150783175995822E-6	0.0985478941644928	0.0
32-33	0.0682596293292513	8.301566351991644E-5	4.150783175995822E-6	4.150783175995822E-6	0.10049046069085885	0.0
34-35	0.07797038656949351	8.301566351991644E-5	4.150783175995822E-6	4.150783175995822E-6	0.10216322631078516	4.150783175995822E-6
36-37	0.08012671842942334	8.301566351991644E-5	4.150783175995822E-6	4.150783175995822E-6	0.10360147268126771	4.150783175995822E-6
38-39	0.0821439990529573	8.301566351991644E-5	4.150783175995822E-6	4.150783175995822E-6	0.10493594947235037	4.150783175995822E-6
40-41	0.0831526393647243	8.509105510791436E-5	4.150783175995822E-6	4.150783175995822E-6	0.10616873207562114	4.150783175995822E-6
42-43	0.08337263087305208	8.924183828391016E-5	4.150783175995822E-6	4.150783175995822E-6	0.10754471669846374	4.150783175995822E-6
44-45	0.08397241904198346	9.3392621459906E-5	4.150783175995822E-6	4.150783175995822E-6	0.10866750354757061	4.150783175995822E-6
46-47	0.08481087724353462	1.1207114575188718E-4	4.150783175995822E-6	4.150783175995822E-6	0.10985047675272942	4.150783175995822E-6
48-49	0.08568669249366975	1.1207114575188718E-4	4.150783175995822E-6	4.150783175995822E-6	0.11082383540750045	4.150783175995822E-6
50-51	0.08650439677934092	1.1207114575188718E-4	4.150783175995822E-6	4.150783175995822E-6	0.11188851129214336	4.150783175995822E-6
52-53	0.08724738696784418	1.1829732051588091E-4	4.150783175995822E-6	4.150783175995822E-6	0.1128473422057984	8.301566351991643E-6
54-55	0.08761888206209581	1.349004532198642E-4	4.150783175995822E-6	4.150783175995822E-6	0.11373560980546152	1.4527741115985375E-5
56-57	0.0881211268263913	1.4320201957185584E-4	4.150783175995822E-6	4.150783175995822E-6	0.11465708367053258	1.6603132703983287E-5
58-59	0.08824357493008317	1.4527741115985375E-4	4.150783175995822E-6	4.150783175995822E-6	0.11549761726367173	2.075391587997911E-5
60-61	0.08844488791411897	1.515035859238475E-4	4.150783175995822E-6	4.150783175995822E-6	0.11629041685028693	2.075391587997911E-5
62-63	0.08863167315703878	1.5772976068784123E-4	4.150783175995822E-6	4.150783175995822E-6	0.11715585514248206	2.075391587997911E-5
64-65	0.08865035168133076	1.5980515227583913E-4	6.226174763993732E-6	4.150783175995822E-6	0.11793412698798128	2.075391587997911E-5
66-67	0.08868770872991472	1.6603132703983287E-4	8.301566351991643E-6	4.150783175995822E-6	0.11869994648395252	2.075391587997911E-5
68-69	0.0887188396037347	1.6810671862783078E-4	8.301566351991643E-6	4.150783175995822E-6	0.11947406754627574	2.075391587997911E-5
70-71	0.08883091074948657	1.7018211021582868E-4	8.301566351991643E-6	4.150783175995822E-6	0.12033120427211887	2.490469905597493E-5
72-73	0.08886411701489455	1.7225750180382661E-4	8.301566351991643E-6	4.150783175995822E-6	0.12098495262233822	3.7357048583962396E-5
74-75	0.08888279553918652	1.7433289339182452E-4	8.301566351991643E-6	4.150783175995822E-6	0.12162002244826557	3.943244017196031E-5
76-77	0.08893052954571048	1.7433289339182452E-4	8.301566351991643E-6	4.150783175995822E-6	0.1224999884815767	4.150783175995822E-5
78-79	0.08898448972699843	2.0131298403579733E-4	8.301566351991643E-6	4.150783175995822E-6	0.1237742789166074	4.565861493595404E-5
80-81	0.08901977138399439	2.137653335637848E-4	8.301566351991643E-6	4.150783175995822E-6	0.12458368163592659	4.565861493595404E-5
82-83	0.08904675147463836	2.1584072515178272E-4	1.0376957939989553E-5	4.150783175995822E-6	0.12531214408331387	4.980939811194986E-5
84-85	0.08907580695687034	2.1584072515178272E-4	1.2452349527987464E-5	4.150783175995822E-6	0.1263353121361968	4.980939811194986E-5
86-87	0.0891090132222783	2.1791611673978062E-4	1.2452349527987464E-5	4.150783175995822E-6	0.127144714855516	4.980939811194986E-5
88-89	0.08912976713815829	2.2206689991577646E-4	1.2452349527987464E-5	4.150783175995822E-6	0.12803090706359113	4.980939811194986E-5
90-91	0.08914637027086225	2.345192494437639E-4	1.2452349527987464E-5	1.0376957939989553E-5	0.1287261632455704	4.980939811194986E-5
92-93	0.08916297340356624	2.3659464103176184E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.12938821316214175	5.81109644639415E-5
94-95	0.08922938593438218	2.3867003261975975E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.13039892886549675	5.81109644639415E-5
96-97	0.08925013985026216	2.4074542420775765E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.13115022062035198	5.81109644639415E-5
98-99	0.08926674298296614	2.448962073837535E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.1318392506275673	5.81109644639415E-5
100-101	0.08929994924837412	2.448962073837535E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.13253658220113457	6.226174763993732E-5
102-103	0.0893165523810781	2.490469905597493E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.13323183838311387	7.056331399192897E-5
104-105	0.08932900473060608	2.511223821477472E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.13394162230620915	7.471409716792479E-5
106-107	0.08934975864648606	2.531977737357451E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.13461197378913248	7.471409716792479E-5
108-109	0.08938504030348202	2.5734855691174097E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.13536741632716373	7.471409716792479E-5
110-111	0.08943069891841798	2.594239484997389E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.13614153738948695	7.471409716792479E-5
112-113	0.08944730205112196	2.677255148517305E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.1369862217658021	7.678948875592271E-5
114-115	0.08946805596700194	2.739516896157242E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.13773543812906935	7.886488034392061E-5
116-117	0.08947635753335392	2.7810247279172007E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.13841824196152067	7.886488034392061E-5
118-119	0.08949711144923392	2.905548223197075E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.1391570813668479	7.886488034392061E-5
120-121	0.0895033376239979	2.9470560549570336E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.14000384113475106	7.886488034392061E-5
122-123	0.0895137145819379	2.9470560549570336E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.14073645436531435	8.094027193191852E-5
124-125	0.08952201614828989	2.9470560549570336E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.14152510316875355	9.131722987190808E-5
126-127	0.08952824232305387	3.0093178025969707E-4	1.2452349527987464E-5	1.2452349527987464E-5	0.14222866091708483	1.0169418781189762E-4
128-129	0.08954069467258186	3.071579550236908E-4	1.6603132703983287E-5	1.2452349527987464E-5	0.1431397578242159	1.0376957939989554E-4
130-131	0.08954899623893385	3.092333466116887E-4	1.6603132703983287E-5	1.2452349527987464E-5	0.14411104108739892	1.0376957939989554E-4
132-133	0.08956767476322583	3.1130873819968665E-4	1.6603132703983287E-5	1.2452349527987464E-5	0.14492044380671812	1.0376957939989554E-4
134-135	0.08958012711275382	3.1130873819968665E-4	1.6603132703983287E-5	1.2452349527987464E-5	0.14566966016998537	1.0792036257589136E-4
136-137	0.08959050407069381	3.1338412978768455E-4	1.6603132703983287E-5	1.2452349527987464E-5	0.1464500074070726	1.0792036257589136E-4
138-139	0.08959050407069381	3.1753491296368036E-4	1.6603132703983287E-5	1.2452349527987464E-5	0.14731752109085572	1.0792036257589136E-4
>>END_MODULE
