##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.4d.S.3.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24166152
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	69-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.48011487306709	36.0	34.0	38.0	31.0	39.0
2	34.8074490303628	35.0	34.0	38.0	31.0	39.0
3	34.95682837714502	35.0	34.0	38.0	31.0	39.0
4	34.9255531455732	36.0	34.0	38.0	31.0	39.0
5	34.79168106697335	35.0	34.0	37.0	31.0	39.0
6	34.77362813078392	35.0	34.0	37.0	31.0	39.0
7	34.75933855749976	35.0	34.0	37.0	32.0	39.0
8	34.92944234564113	35.0	34.0	37.0	32.0	39.0
9	34.913704548411346	35.0	34.0	37.0	32.0	39.0
10-14	34.889346818641215	35.0	34.0	37.4	31.6	39.0
15-19	34.88641446929573	35.0	34.0	37.0	31.0	39.0
20-24	34.89495264285352	35.0	34.0	37.0	31.0	39.0
25-29	34.88673835205539	35.0	34.0	37.0	31.0	39.0
30-34	34.86850134849768	35.0	34.0	37.0	31.0	39.0
35-39	34.86445298366078	35.0	34.0	37.0	31.0	39.0
40-44	34.79951614141962	35.0	34.0	37.0	31.0	39.0
45-49	34.698302385915646	35.0	34.0	37.0	31.0	39.0
50-54	34.66530926396557	35.0	34.0	37.0	31.0	39.0
55-59	34.65499794919771	35.0	34.0	37.0	31.0	39.0
60-64	34.62627716650958	35.0	34.0	37.0	31.0	39.0
65-69	34.60677296906847	35.0	34.0	37.0	30.6	39.0
70-74	34.579434469595626	35.0	34.0	37.0	30.0	39.0
75-79	34.559039769026036	35.0	34.0	37.0	30.0	39.0
80-84	34.52991708579306	35.0	34.0	37.0	30.0	39.0
85-89	34.51209001057218	35.0	34.0	37.0	30.0	39.0
90-94	34.48524631564165	35.0	34.0	37.0	30.0	39.0
95-99	34.447671943644934	35.0	34.0	37.0	30.0	39.0
100-104	34.41185156582679	35.0	34.0	37.0	30.0	39.0
105-109	34.353534023699765	35.0	34.0	37.0	30.0	39.0
110-114	34.321782907892015	35.0	34.0	37.0	30.0	39.0
115-119	34.271830172550196	35.0	34.0	37.0	30.0	39.0
120-124	34.23621801455632	35.0	34.0	37.0	29.6	39.0
125-129	34.17980922180203	35.0	34.0	37.0	29.0	39.0
130-134	34.131814872977024	35.0	34.0	37.0	29.0	39.0
135-139	34.08171480825174	35.0	34.0	37.0	29.0	39.0
140-144	34.05918967558565	35.0	34.0	37.0	29.0	39.0
145-149	34.06451377111845	35.0	34.0	37.0	29.0	39.0
150	34.98133166096167	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	11.0
22	287.0
23	3376.0
24	22651.0
25	88997.0
26	218522.0
27	388924.0
28	564697.0
29	735744.0
30	915072.0
31	1145506.0
32	1462207.0
33	1921186.0
34	2653680.0
35	4130744.0
36	8515625.0
37	1398921.0
38	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.07985466614627	10.406931976592714	15.537008126076508	38.97620523118451
2	23.007644879227776	12.205472365826674	36.544485788383575	28.242396966561977
3	21.64570255548869	17.196783714867838	28.24304054172323	32.91447318792024
4	27.02660461347024	24.913421241706423	21.880269121924982	26.179705022898354
5	26.745041895190624	30.57298064866713	23.939310288664647	18.742667167477602
6	19.73727115286799	36.0005523432546	25.44189529379381	18.8202812100836
7	16.607938378457025	24.315948617927248	40.114910585053586	18.961202418562138
8	19.16359894387741	23.60080019418911	31.268253333363127	25.967347528570357
9	20.047953058935825	22.51336612274707	32.4726260506801	24.966054767637004
10-14	20.970088249353182	28.572501029416614	26.72167972983394	23.735730991396267
15-19	21.214784671591747	27.453653897958752	27.3569473945124	23.9746140359371
20-24	21.151134776750872	27.540611713448897	27.26177918763042	24.04647432216981
25-29	21.11644615856886	27.739519907964834	26.93121210657602	24.212821826890288
30-34	21.12836824893102	27.503271150581725	27.1502349225761	24.21812567791115
35-39	21.424127931422515	27.265550690321493	27.051755856940034	24.258565521315962
40-44	21.577917244670576	27.382623328688116	26.868669930236983	24.17078949640433
45-49	21.81358696676792	27.321809423255573	26.778104549886102	24.086499060090404
50-54	21.805197097776855	27.534456844497313	26.696693945458804	23.96365211226703
55-59	21.714131599711152	27.49170104862582	26.766245751914962	24.027921599748066
60-64	21.9231394200755	27.320680956346603	26.566306341835215	24.18987328174268
65-69	21.766649360585788	27.419600277450133	26.7552887638894	24.058461598074675
70-74	22.13186162997091	27.180939209235618	26.686295182447907	24.00090397834557
75-79	22.000960718669603	27.067982854444107	26.677508200793675	24.253548226092615
80-84	22.235827436309876	27.07816960064217	26.69388191335338	23.992121049694575
85-89	22.100001874533927	27.064251036590363	26.713175246535346	24.12257184234036
90-94	22.10227450804614	26.835169115639435	26.698111840428208	24.364444535886214
95-99	22.308621492938048	26.84870871246403	26.87739291023859	23.965276884359334
100-104	22.255432526005396	26.70272043873983	26.92381560229523	24.118031432959544
105-109	22.34414721956099	26.65904832452572	26.822723199977144	24.174081255936148
110-114	22.327551277621694	26.723933129916194	26.792559662315746	24.155955930146366
115-119	22.424423254109342	26.886827955080356	26.70990443161848	23.97884435919182
120-124	22.382896756709563	26.750220613774694	26.7021595316568	24.164723097858943
125-129	22.74880381406916	26.721835561007516	26.583880494870037	23.945480130053284
130-134	22.505615646174807	26.987438994279945	26.46694993593901	24.039995423606232
135-139	22.58270364646738	26.811088609630524	26.54009218945651	24.066115554445588
140-144	22.563795815729897	26.638980941267796	26.79400122963943	24.003222013362873
145-149	21.99721396802845	26.715102828773386	27.131948498916913	24.155734704281247
150	21.856602110817892	25.283288714113905	27.829476391167923	25.030632783900273
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.5
10	2.0
11	4.5
12	9.5
13	15.5
14	19.0
15	41.5
16	91.0
17	197.0
18	459.5
19	836.5
20	1358.0
21	2180.0
22	3660.5
23	6100.0
24	9950.5
25	15454.0
26	22322.5
27	32459.5
28	50210.0
29	74886.5
30	102591.0
31	130399.5
32	158519.0
33	191489.0
34	227716.5
35	275835.5
36	345247.0
37	419661.5
38	495311.5
39	605620.0
40	754845.0
41	917342.5
42	1085931.0
43	1251523.0
44	1397418.0
45	1506914.0
46	1577124.5
47	1590424.5
48	1539800.5
49	1482935.5
50	1390299.5
51	1230821.5
52	1038224.5
53	867392.0
54	723304.5
55	595474.5
56	498289.5
57	404169.5
58	330207.5
59	267919.0
60	201249.0
61	137990.5
62	82042.5
63	48112.5
64	30991.5
65	18949.0
66	12158.5
67	5975.0
68	2401.0
69	1119.5
70	858.5
71	604.5
72	332.0
73	144.0
74	91.0
75	62.0
76	35.0
77	30.5
78	15.5
79	3.5
80	2.5
81	1.0
82	0.5
83	0.0
84	0.0
85	0.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.6965878556089527E-4
3	4.5518210760240186E-5
4	2.069009580010918E-5
5	5.3794249080283865E-5
6	8.276038320043671E-5
7	2.4000511128126643E-4
8	7.448434488039304E-5
9	7.862236404041487E-5
10-14	1.5393431275281228E-4
15-19	1.737968047209171E-4
20-24	1.3655463228072056E-4
25-29	3.542144400978691E-4
30-34	7.870512442361532E-4
35-39	7.440158449719261E-4
40-44	9.33537122500926E-4
45-49	8.722944389326029E-4
50-54	5.213904141627512E-4
55-59	3.591800630898953E-4
60-64	2.8304051054549355E-4
65-69	3.302139289697425E-4
70-74	3.484212449928619E-4
75-79	2.7724739156343467E-4
80-84	2.491090626787368E-4
85-89	3.434564714440772E-4
90-94	3.873202441987931E-4
95-99	3.1283628607613775E-4
100-104	4.8580830221229074E-4
105-109	2.904933830655798E-4
110-114	2.309063956618995E-4
115-119	2.524268420043926E-4
120-124	2.2677297463697391E-4
125-129	1.4649458810919483E-4
130-134	1.5974147902533134E-4
135-139	1.6057942220560622E-4
140-144	1.7306239589965668E-4
145-149	1.6287721571982527E-4
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
68-69	1.0
70-71	1.0
72-73	2.0
74-75	3.0
76-77	4.0
78-79	10.0
80-81	9.0
82-83	9.0
84-85	17.0
86-87	13.0
88-89	12.0
90-91	19.0
92-93	26.0
94-95	21.0
96-97	20.0
98-99	31.0
100-101	47.0
102-103	37.0
104-105	56.0
106-107	62.0
108-109	56.0
110-111	54.0
112-113	78.0
114-115	98.0
116-117	94.0
118-119	109.0
120-121	124.0
122-123	141.0
124-125	169.0
126-127	211.0
128-129	245.0
130-131	290.0
132-133	441.0
134-135	564.0
136-137	990.0
138-139	2276.0
140-141	6007.0
142-143	9199.0
144-145	70696.0
146-147	775311.0
148-149	1411917.0
150-151	2.1886682E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.83647087504639
#Duplication Level	Percentage of total
1	33.10856406111327
2	9.063644389507514
3	5.257445468140094
4	3.731633469228986
5	2.692573519542512
6	2.159877459190437
7	1.825700044600491
8	1.4773298804944717
9	1.2851216137248151
>10	16.523544062496036
>50	5.542250775363871
>100	10.252310300478015
>500	3.282369733493098
>1k	3.3055812994750333
>5k	0.38253565411535034
>10k+	0.10951826903617208
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	8.276038320043671E-6	0.0	0.0	4.138019160021836E-6	3.1862747532168136E-4	2.896613412015285E-5
2	1.6552076640087343E-5	4.551821076024019E-5	0.0	1.6552076640087343E-5	0.0023752229978525334	3.724217244019652E-5
3	2.069009580010918E-5	5.79322682403057E-5	0.0	1.6552076640087343E-5	0.00462216740174439	5.3794249080283865E-5
4	4.965622992026203E-5	6.620830656034937E-5	0.0	1.6552076640087343E-5	0.006687038962595286	6.620830656034937E-5
5	5.79322682403057E-5	6.620830656034937E-5	0.0	2.069009580010918E-5	0.007940858768081902	6.620830656034937E-5
6	7.448434488039304E-5	7.03463257203712E-5	0.0	2.4828114960131014E-5	0.009600204451250658	6.620830656034937E-5
7	9.517444068050222E-5	9.517444068050222E-5	0.0	2.4828114960131014E-5	0.011077477291378453	6.620830656034937E-5
8	1.862108622009826E-4	9.517444068050222E-5	0.0	2.4828114960131014E-5	0.01442927281099614	7.03463257203712E-5
9	2.648332262413975E-4	9.931245984052406E-5	0.0	2.4828114960131014E-5	0.017503821046892364	7.03463257203712E-5
10-11	3.227654944817032E-4	1.0345047900054589E-4	0.0	2.6897124540141932E-5	0.03025926510765967	1.0345047900054589E-4
12-13	3.620766765019106E-4	1.0345047900054589E-4	0.0	2.896613412015285E-5	0.03914152323464654	1.386236418607315E-4
14-15	3.9311182020207436E-4	1.0965750774057865E-4	0.0	2.896613412015285E-5	0.045549245903940355	1.4896868976078609E-4
16-17	4.241469639022381E-4	1.2000255564063323E-4	0.0	2.896613412015285E-5	0.04957140052748158	1.51037699340797E-4
18-19	4.862172513025657E-4	1.282785939606769E-4	0.0	2.896613412015285E-5	0.05292526505667928	1.613827472408516E-4
20-21	7.634645350240287E-4	1.531067089208079E-4	0.0	3.1035143700163765E-5	0.05549497495505283	1.6965878556089524E-4
22-23	0.0010220907325253934	1.5724472808082974E-4	0.0	3.517316286018561E-5	0.058408140443708206	1.6965878556089524E-4
24-25	0.00110485111572583	1.634517568208625E-4	0.0	4.138019160021836E-5	0.06108130082108231	1.6965878556089524E-4
26-27	0.0011462313073260485	1.6552076640087343E-4	0.0	4.344920118022928E-5	0.06352687014465522	1.8207284304096075E-4
28-29	0.0012020945659863432	1.6552076640087343E-4	0.0	6.207028740032753E-5	0.06617106438790918	1.9034888136100443E-4
30-31	0.001309683064146911	1.6552076640087343E-4	0.0	8.689840236045854E-5	0.06920009441304516	2.0690095800109177E-4
32-33	0.0013531322653271402	1.6965878556089524E-4	0.0	8.689840236045854E-5	0.07204705159514017	2.8345431246149574E-4
34-35	0.0014420996772676097	1.7172779514090618E-4	0.0	8.689840236045854E-5	0.07465814168511395	2.9793737952157217E-4
36-37	0.0014752038305477842	1.75865814300928E-4	0.0	8.896741194046946E-5	0.07706439982666666	3.02075398681594E-4
38-39	0.0014979629359279044	1.8207284304096077E-4	0.0	9.517444068050222E-5	0.07998997937280208	3.0621341784161586E-4
40-41	0.0015289980796280682	1.8414185262097168E-4	0.0	9.517444068050222E-5	0.08235485732275458	3.124204465816486E-4
42-43	0.0015310670892080792	1.882798717809935E-4	0.0	9.517444068050222E-5	0.08463904389908664	3.144894561616595E-4
44-45	0.0015476191658481664	1.9034888136100443E-4	0.0	9.724345026051314E-5	0.08733289437226084	3.144894561616595E-4
46-47	0.0015703782712282867	1.9034888136100443E-4	0.0	9.931245984052406E-5	0.09173781576810408	3.144894561616595E-4
48-49	0.0015910683670283957	1.9862491968104812E-4	0.0	9.931245984052406E-5	0.09440063109757812	3.227654944817032E-4
50-51	0.001609689453248494	2.1517699632113546E-4	0.0	9.931245984052406E-5	0.09717724195395278	3.227654944817032E-4
52-53	0.0016303795490486032	2.1517699632113546E-4	0.0	9.931245984052406E-5	0.09951315376978512	3.3104153280174686E-4
54-55	0.0016676217214887997	2.1517699632113546E-4	0.0	9.931245984052406E-5	0.10133802021935474	3.351795519617687E-4
56-57	0.0017296920088891274	2.213840250611682E-4	0.0	9.931245984052406E-5	0.1033015103107851	3.372485615417796E-4
58-59	0.0017979693250294874	2.3793610170125555E-4	0.0	1.0965750774057865E-4	0.10535603682373595	3.4345559028181236E-4
60-61	0.0018807297082299242	2.4000511128126646E-4	0.0	1.158645364806114E-4	0.10735883809718652	3.4345559028181236E-4
62-63	0.0019324549477301972	2.4621214002129924E-4	0.0	1.158645364806114E-4	0.10974647515251912	3.4345559028181236E-4
64-65	0.001955214053110317	2.482811496013101E-4	0.0	1.2414057480065506E-4	0.11230997802215265	3.4345559028181236E-4
66-67	0.0019738351393304154	2.648332262413975E-4	0.0	1.282785939606769E-4	0.114654165876305	3.4345559028181236E-4
68-69	0.0019821111776504594	2.7517827414145205E-4	0.0	1.282785939606769E-4	0.11655972369949506	3.5173162860185604E-4
70-71	0.0020131463213506227	2.77247283721463E-4	0.0	1.282785939606769E-4	0.11861838823160592	3.5173162860185604E-4
72-73	0.002017284340510645	2.896613412015285E-4	0.0	1.282785939606769E-4	0.12056946426555622	3.5173162860185604E-4
74-75	0.002025560378830688	2.896613412015285E-4	0.0	1.3655463228072056E-4	0.12220605084334485	3.53800638181867E-4
76-77	0.0020503884937908196	2.896613412015285E-4	0.0	1.3655463228072056E-4	0.12352814796497183	3.5586964776187786E-4
78-79	0.0020814236374909834	2.896613412015285E-4	0.0	1.551757185008188E-4	0.12472610451179816	3.5586964776187786E-4
80-81	0.00211866580993118	2.9173035078153937E-4	4.138019160021836E-6	1.5724472808082974E-4	0.12607096073880525	3.5586964776187786E-4
82-83	0.002139355905731289	2.937993603615503E-4	4.138019160021836E-6	1.613827472408516E-4	0.1276309939621335	3.5586964776187786E-4
84-85	0.0021765980781714854	2.937993603615503E-4	4.138019160021836E-6	1.6552076640087343E-4	0.12923447638664193	3.600076669218997E-4
86-87	0.002215909260191693	2.9793737952157217E-4	4.138019160021836E-6	1.6552076640087343E-4	0.1306662310160095	3.600076669218997E-4
88-89	0.002238668365571813	2.9793737952157217E-4	4.138019160021836E-6	1.7172779514090618E-4	0.13205867446335684	3.620766765019106E-4
90-91	0.002251082423051879	3.0621341784161586E-4	4.138019160021836E-6	1.737968047209171E-4	0.13409251088050758	3.6414568608192154E-4
92-93	0.0022717725188519874	3.144894561616595E-4	4.138019160021836E-6	1.737968047209171E-4	0.13596496455041746	3.6414568608192154E-4
94-95	0.0023255667679322714	3.2069648490169224E-4	4.138019160021836E-6	1.737968047209171E-4	0.1375415498503858	3.662146956619325E-4
96-97	0.0023876370553325994	3.289725232217359E-4	4.138019160021836E-6	1.737968047209171E-4	0.13884088786663262	3.7035271482195424E-4
98-99	0.002466259419373014	3.3311054238175774E-4	4.138019160021836E-6	1.737968047209171E-4	0.13984849553209794	3.724217244019652E-4
100-101	0.002567640888793549	3.351795519617687E-4	4.138019160021836E-6	1.75865814300928E-4	0.140994726839424	3.724217244019652E-4
102-103	0.002590399994173669	3.393175711217905E-4	4.138019160021836E-6	1.7793482388093893E-4	0.14208302587850974	3.744907339819761E-4
104-105	0.0026152281091338	3.4345559028181236E-4	4.138019160021836E-6	1.8621086220098261E-4	0.14318580798465558	3.78628753141998E-4
106-107	0.002648332262413975	3.4552459986182324E-4	4.138019160021836E-6	1.9241789094101536E-4	0.14430514216744145	3.8069776272200886E-4
108-109	0.0026710913677940947	3.51731628601856E-4	4.138019160021836E-6	1.9862491968104812E-4	0.14544516644602748	3.8069776272200886E-4
110-111	0.002714540568974324	3.620766765019106E-4	4.138019160021836E-6	1.9862491968104812E-4	0.14646725717855288	3.8276677230201974E-4
112-113	0.002786955904274706	3.6414568608192154E-4	4.138019160021836E-6	2.0276293884106993E-4	0.14761762650503896	3.848357818820307E-4
114-115	0.0028262670862949134	3.6828370524194336E-4	4.138019160021836E-6	2.0276293884106993E-4	0.1488362731476654	3.8897380104205255E-4
116-117	0.0028841993545352194	3.76559743561987E-4	4.138019160021836E-6	2.0690095800109177E-4	0.14977146547783032	3.8897380104205255E-4
118-119	0.0029317865748754706	3.8483578188203073E-4	4.138019160021836E-6	2.1724600590114637E-4	0.15067769167387507	3.8897380104205255E-4
120-121	0.003064203187996169	3.8897380104205255E-4	4.138019160021836E-6	2.2759105380120096E-4	0.15165012617648022	3.8897380104205255E-4
122-123	0.003302139289697425	3.9311182020207436E-4	4.138019160021836E-6	2.317290729612228E-4	0.15251290317134478	3.8897380104205255E-4
124-125	0.003434555902818124	3.9311182020207436E-4	4.138019160021836E-6	2.4000511128126646E-4	0.1533508520512492	3.8897380104205255E-4
126-127	0.0034780051039983526	3.951808297820853E-4	4.138019160021836E-6	2.441431304412883E-4	0.1541888009311536	3.8897380104205255E-4
128-129	0.0035317993530786366	3.993188489421071E-4	4.138019160021836E-6	2.52419168761332E-4	0.15490054022667737	3.910428106220635E-4
130-131	0.003664215966199335	4.0138785852211805E-4	4.138019160021836E-6	2.6276421666138655E-4	0.15559158942640103	3.9311182020207436E-4
132-133	0.0037759424835199246	4.0552587768213986E-4	4.138019160021836E-6	2.689712454014193E-4	0.15628263862612468	3.9311182020207436E-4
134-135	0.0038545648475603396	4.0552587768213986E-4	4.138019160021836E-6	2.689712454014193E-4	0.15712058750602909	3.9311182020207436E-4
136-137	0.003943532259500809	4.2207795432222723E-4	4.138019160021836E-6	2.7104025498143024E-4	0.1578799140218931	3.951808297820853E-4
138-139	0.003947670278660831	4.2621597348224905E-4	4.138019160021836E-6	2.731092645614411E-4	0.1585626871832967	3.9724983936209623E-4
>>END_MODULE
