##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.4d.S.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24166152
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	64-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.02855688402523	37.0	35.0	38.0	33.0	39.0
2	35.36919762815363	35.0	34.0	37.0	33.0	39.0
3	35.48677960810641	35.0	34.0	37.0	33.0	39.0
4	35.565379750983936	36.0	35.0	38.0	33.0	39.0
5	35.48718616848888	36.0	35.0	38.0	33.0	39.0
6	35.435494488324	35.0	35.0	37.0	33.0	39.0
7	35.46287079548287	35.0	34.0	38.0	33.0	39.0
8	35.56937649816984	36.0	35.0	38.0	33.0	39.0
9	35.55574755964458	36.0	35.0	38.0	33.0	39.0
10-14	35.52782908093933	35.8	35.0	37.8	33.0	39.0
15-19	35.50657703386125	36.0	35.0	38.0	33.0	39.0
20-24	35.49456829535791	36.0	35.0	38.0	33.0	39.0
25-29	35.47729162673478	36.0	34.8	38.0	33.0	39.0
30-34	35.462311144943556	36.0	34.4	38.0	33.0	39.0
35-39	35.43326054557631	36.0	34.0	38.0	33.0	39.0
40-44	35.41778907125967	36.0	34.0	38.0	33.0	39.0
45-49	35.390759050096186	36.0	34.0	38.0	32.8	39.0
50-54	35.36949006196767	36.0	34.0	38.0	32.4	39.0
55-59	35.34644722088978	36.0	34.0	38.0	32.0	39.0
60-64	35.304689211588176	36.0	34.0	38.0	32.0	39.0
65-69	35.2778808052191	36.0	34.0	38.0	32.0	39.0
70-74	35.2453795587871	36.0	34.0	38.0	32.0	39.0
75-79	35.19752595114649	36.0	34.0	38.0	32.0	39.0
80-84	35.157316555965686	36.0	34.0	38.0	32.0	39.0
85-89	35.10944186780412	36.0	34.0	38.0	32.0	39.0
90-94	35.057397480671696	35.8	34.0	38.0	32.0	39.0
95-99	34.99222979100874	35.2	34.0	38.0	31.0	39.0
100-104	34.94494268394108	35.0	34.0	38.0	31.0	39.0
105-109	34.886432443357954	35.0	34.0	37.2	31.0	39.0
110-114	34.81251793486366	35.0	34.0	37.0	31.0	39.0
115-119	34.73839850055761	35.0	34.0	37.0	31.0	39.0
120-124	34.66539157993175	35.0	34.0	37.0	30.2	39.0
125-129	34.58647456175797	35.0	34.0	37.0	30.0	39.0
130-134	34.50001339005438	35.0	34.0	37.0	30.0	39.0
135-139	34.42749887458428	35.0	34.0	37.0	30.0	39.0
140-144	34.305411117409854	35.0	34.0	37.0	29.2	39.0
145-149	34.293445357009816	35.0	34.0	37.0	29.2	39.0
150	35.08095443493481	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	11.0
21	171.0
22	1603.0
23	8857.0
24	30696.0
25	75560.0
26	147435.0
27	251620.0
28	370078.0
29	489549.0
30	627513.0
31	814583.0
32	1091367.0
33	1518355.0
34	2273779.0
35	3860689.0
36	1.0333518E7
37	2270763.0
38	4.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.61139994484848	18.794121629293734	15.65156091048339	31.942917515374397
2	24.513447569145473	25.54045426843297	32.64271448760233	17.303383674819226
3	19.69042485539278	29.505773198811298	29.884778511696858	20.919023434099067
4	23.318160044677366	34.33506087357226	21.650530047150244	20.696249034600132
5	25.243464495299044	35.18214236176285	21.689911575496172	17.88448156744193
6	18.905939183035844	39.02056479657994	22.03751759899549	20.035978421388727
7	20.373636646827347	20.583665947313413	37.159838272969566	21.88285913288967
8	21.400668174229807	23.2602650186095	28.273197983692235	27.06586882346846
9	23.53887784865377	23.427900312801146	27.707493522344805	25.32572831620028
10-14	23.061155123082898	28.771455215542797	25.30866312518435	22.85872653618996
15-19	23.062214455987863	27.65083990202495	26.85879075824732	22.428154883739868
20-24	22.987174788936198	27.640724927990192	26.763829011751643	22.60827127132197
25-29	23.08385712379861	27.254568290392285	26.756352438733316	22.905222147075794
30-34	22.9558259833837	27.002735892747843	26.960232642747588	23.08120548112087
35-39	23.239156155270397	26.74982678251796	27.01976797961049	22.991249082601154
40-44	23.421537694540696	26.398410470976096	27.067068849024867	23.112982985458338
45-49	23.316581804169733	26.25245839718297	27.1085425598581	23.322417238789196
50-54	23.56553496808263	26.29156433345284	26.804921197218324	23.337979501246206
55-59	23.597498683282303	26.338315673922768	26.808253957849804	23.255931684945125
60-64	23.305285839466706	26.315028557297826	26.88643769185926	23.49324791137621
65-69	23.539675367246947	26.249557511240535	26.859518122840797	23.35124899867172
70-74	23.686228094518615	26.236229536205013	26.57268785860708	23.50485451066929
75-79	23.497060840280533	26.367372946508766	26.826091925388816	23.30947428782189
80-84	23.813124959374893	26.365142185098467	26.515917088253264	23.305815767273373
85-89	23.541751889619846	26.435435918281435	26.737395386600777	23.285416805497942
90-94	23.585649862664084	26.19045682042681	26.753885604811632	23.470007712097473
95-99	23.594568449296478	26.353649658986022	26.77327685104921	23.278505040668286
100-104	23.82196031065344	26.191797274619294	26.69569169833223	23.290550716395032
105-109	23.62850876217248	26.383073119229582	26.594951862277803	23.393466256320128
110-114	23.721896814523316	26.48922473793584	26.482571323583855	23.30630712395699
115-119	23.869568597466465	26.544617366889717	26.65443490408125	22.93137913156257
120-124	23.827573843444082	26.681078623828675	26.779571835771694	22.711775696955556
125-129	23.79539284589117	26.758381761840592	26.627436345686263	22.818789046581976
130-134	23.94344314511286	26.767242375096938	26.643503385613478	22.645811094176725
135-139	23.69171319327438	26.780458305715438	26.93106493550486	22.596763565505317
140-144	23.749661552115764	26.91260213559714	26.70235341060007	22.63538290168702
145-149	23.556155659442844	27.14075914632718	26.537837032550982	22.76524816167899
150	23.728022326388484	26.000668528548516	26.80201213508415	23.469297009978852
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.5
9	2.5
10	2.5
11	2.5
12	4.5
13	10.0
14	18.0
15	28.5
16	49.0
17	84.0
18	140.0
19	241.5
20	395.5
21	593.5
22	913.0
23	1561.5
24	2592.0
25	4251.0
26	6642.5
27	10301.5
28	15977.0
29	23088.0
30	33098.0
31	48186.0
32	69431.0
33	96117.5
34	132149.0
35	187879.0
36	266795.5
37	363658.0
38	484328.5
39	630849.5
40	804016.5
41	991759.5
42	1183569.0
43	1379305.0
44	1532975.0
45	1625005.0
46	1650188.5
47	1615458.0
48	1563070.0
49	1505010.0
50	1391721.0
51	1227450.0
52	1042207.5
53	868769.5
54	736583.5
55	630933.5
56	513915.0
57	399672.5
58	327598.5
59	266383.5
60	197148.0
61	131446.0
62	82735.5
63	51215.5
64	31463.0
65	15921.0
66	8786.5
67	5016.0
68	3130.0
69	1962.5
70	815.5
71	519.0
72	333.5
73	256.0
74	209.5
75	126.0
76	82.0
77	37.5
78	16.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	8.276059210403536E-7
85-89	0.0
90-94	0.0
95-99	0.0
100-104	8.276234830582286E-7
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	3.310799014411552E-6
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
64-65	1.0
66-67	0.0
68-69	2.0
70-71	4.0
72-73	10.0
74-75	6.0
76-77	9.0
78-79	9.0
80-81	18.0
82-83	23.0
84-85	29.0
86-87	31.0
88-89	35.0
90-91	46.0
92-93	49.0
94-95	68.0
96-97	59.0
98-99	80.0
100-101	94.0
102-103	101.0
104-105	96.0
106-107	102.0
108-109	123.0
110-111	123.0
112-113	123.0
114-115	188.0
116-117	192.0
118-119	178.0
120-121	244.0
122-123	266.0
124-125	299.0
126-127	357.0
128-129	453.0
130-131	573.0
132-133	635.0
134-135	886.0
136-137	1526.0
138-139	4135.0
140-141	8425.0
142-143	9743.0
144-145	62844.0
146-147	800525.0
148-149	1042529.0
150-151	2.2230913E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.241538463003785
#Duplication Level	Percentage of total
1	25.57727685917794
2	8.49823451300169
3	5.278109461893337
4	3.860989416911562
5	2.9257123220250745
6	2.262686106061685
7	1.8680270510251549
8	1.65667271916434
9	1.421982201217782
>10	17.493987513248804
>50	6.0487671969200605
>100	12.414068075207597
>500	3.7160523575665545
>1k	5.322523105258248
>5k	0.9165452608167096
>10k+	0.7383658405036232
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	35373	0.14637415174745239	No Hit
GAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCCG	24173	0.10002833715520781	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.062134178416158E-4	0.0	0.0	0.0	0.0017793482388093892	0.0
2	0.001688311817288909	4.138019160021836E-6	0.0	4.138019160021836E-6	0.008040171227922426	0.0
3	0.0024579833810529704	4.138019160021836E-6	0.0	4.138019160021836E-6	0.011785078567742188	0.0
4	0.005731156536630242	4.138019160021836E-6	0.0	4.138019160021836E-6	0.017710722004893455	0.0
5	0.005958747590431443	4.138019160021836E-6	0.0	4.138019160021836E-6	0.02152597567043359	0.0
6	0.007063598706157273	4.138019160021836E-6	0.0	4.138019160021836E-6	0.028738543066351648	0.0
7	0.009070537998767864	8.276038320043671E-6	0.0	4.138019160021836E-6	0.0381111564638011	0.0
8	0.015343775045360965	8.276038320043671E-6	0.0	4.138019160021836E-6	0.052701812022038094	0.0
9	0.015856889421203675	8.276038320043671E-6	0.0	4.138019160021836E-6	0.0627075423509709	0.0
10-11	0.016585180793367515	8.276038320043671E-6	0.0	4.138019160021836E-6	0.10453877803963163	0.0
12-13	0.017327955232591435	1.034504790005459E-5	0.0	4.138019160021836E-6	0.12304606873282928	0.0
14-15	0.02025146576914686	1.6552076640087343E-5	0.0	4.138019160021836E-6	0.13202143229091665	0.0
16-17	0.02355360505884429	1.6552076640087343E-5	0.0	4.138019160021836E-6	0.13928572492633498	0.0
18-19	0.03030064529925989	1.862108622009826E-5	0.0	4.138019160021836E-6	0.14653760350427325	0.0
20-21	0.035988352634709904	2.896613412015285E-5	0.0	4.138019160021836E-6	0.15154046866873966	0.0
22-23	0.03826426317272191	4.138019160021836E-5	0.0	4.138019160021836E-6	0.15663850827388653	0.0
24-25	0.04382782993337127	4.758722034025111E-5	0.0	4.138019160021836E-6	0.16101239452602964	0.0
26-27	0.04872517560925711	5.586325866029478E-5	0.0	4.138019160021836E-6	0.1645876430802885	0.0
28-29	0.06037576855429859	5.79322682403057E-5	0.0	4.138019160021836E-6	0.16791667949452604	0.0
30-31	0.0829652151488578	5.79322682403057E-5	0.0	4.138019160021836E-6	0.17132640728238407	0.0
32-33	0.10117043044337386	6.827731614036028E-5	0.0	4.138019160021836E-6	0.1741216392249788	0.0
34-35	0.11426105405610293	7.03463257203712E-5	0.0	4.138019160021836E-6	0.17685273187059322	0.0
36-37	0.11636937481813406	7.03463257203712E-5	0.0	4.138019160021836E-6	0.17893415550808422	0.0
38-39	0.11837217609158462	7.03463257203712E-5	0.0	4.138019160021836E-6	0.1805252238751126	0.0
40-41	0.11969013519405158	7.448434488039304E-5	0.0	4.138019160021836E-6	0.18224043281694163	2.069009580010918E-6
42-43	0.11995703742987299	8.06913736204258E-5	0.0	4.138019160021836E-6	0.18400529798869097	8.276038320043671E-6
44-45	0.1207742962139773	8.689840236045854E-5	0.0	4.138019160021836E-6	0.18549912290545884	8.276038320043671E-6
46-47	0.12214398055594453	9.103642152048039E-5	0.0	4.138019160021836E-6	0.18707363919584716	1.034504790005459E-5
48-49	0.12333366106445082	9.931245984052406E-5	0.0	4.138019160021836E-6	0.18829642385763362	1.2414057480065507E-5
50-51	0.12434126872991613	9.931245984052406E-5	2.069009580010918E-6	4.138019160021836E-6	0.18957920979724036	1.2414057480065507E-5
52-53	0.12542956776900188	9.931245984052406E-5	4.138019160021836E-6	4.138019160021836E-6	0.1907192340758264	1.2414057480065507E-5
54-55	0.126025442528045	1.158645364806114E-4	1.034504790005459E-5	8.276038320043671E-6	0.19178891202869203	1.2414057480065507E-5
56-57	0.12689442655164962	1.2000255564063323E-4	1.4483067060076425E-5	8.276038320043671E-6	0.19287721106777778	1.4483067060076425E-5
58-59	0.12703718821267035	1.2000255564063323E-4	1.6552076640087343E-5	8.276038320043671E-6	0.1937896442925626	1.6552076640087343E-5
60-61	0.12737443677421212	1.2207156522064415E-4	1.6552076640087343E-5	8.276038320043671E-6	0.19450345259766638	2.069009580010918E-5
62-63	0.1276475460387736	1.2414057480065506E-4	1.6552076640087343E-5	8.276038320043671E-6	0.19552554333019173	2.4828114960131014E-5
64-65	0.12770340929743387	1.282785939606769E-4	1.6552076640087343E-5	8.276038320043671E-6	0.1964607356603567	2.4828114960131014E-5
66-67	0.12781927383391448	1.4689968018077518E-4	1.6552076640087343E-5	8.276038320043671E-6	0.1971269567451202	2.4828114960131014E-5
68-69	0.12789375817879486	1.4896868976078609E-4	1.6552076640087343E-5	8.276038320043671E-6	0.19786973118434412	2.4828114960131014E-5
70-71	0.12797444955241527	1.531067089208079E-4	1.6552076640087343E-5	8.276038320043671E-6	0.1986869899684484	2.4828114960131014E-5
72-73	0.12803445083023562	1.531067089208079E-4	2.4828114960131014E-5	1.034504790005459E-5	0.19927665769875152	2.4828114960131014E-5
74-75	0.12806341696435578	1.551757185008188E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.19999667303259533	3.3104153280174686E-5
76-77	0.128108935175116	1.634517568208625E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.2009153132861202	3.3104153280174686E-5
78-79	0.12816479843377632	1.6552076640087343E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.20199326727730588	3.3104153280174686E-5
80-81	0.12821238565411655	1.7793482388093893E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.20278362893687005	4.138019160021836E-5
82-83	0.12823307574991666	1.7793482388093893E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.2036484749413146	4.138019160021836E-5
84-85	0.12827031792235685	1.882798717809935E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.20452159698407923	4.138019160021836E-5
86-87	0.12832825019059715	1.9034888136100443E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.20541540912264394	4.138019160021836E-5
88-89	0.12838411344925743	1.9034888136100443E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.20622646087800822	4.758722034025111E-5
90-91	0.12839652750673752	1.9655591010103718E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.20683061167537142	4.965622992026203E-5
92-93	0.1284130795833776	2.0276293884106993E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.20737683020449427	4.965622992026203E-5
94-95	0.12844618373665778	2.0690095800109177E-4	2.4828114960131014E-5	1.2414057480065507E-5	0.208076155442538	5.586325866029478E-5
96-97	0.12845445977497782	2.0690095800109177E-4	2.6897124540141932E-5	1.4483067060076425E-5	0.20867823723032114	5.79322682403057E-5
98-99	0.12846894284203791	2.0896996758110268E-4	2.896613412015285E-5	1.6552076640087343E-5	0.20925135288398417	6.207028740032753E-5
100-101	0.12850411600489808	2.1517699632113543E-4	2.896613412015285E-5	1.6552076640087343E-5	0.20972308706822668	6.207028740032753E-5
102-103	0.12852687511027822	2.1931501548115727E-4	3.1035143700163765E-5	1.6552076640087343E-5	0.21024654649196944	6.413929698033845E-5
104-105	0.12854963421565835	2.1931501548115727E-4	3.3104153280174686E-5	1.6552076640087343E-5	0.21080311006899238	6.827731614036028E-5
106-107	0.12858273836893852	2.2345303464117912E-4	3.3104153280174686E-5	1.6552076640087343E-5	0.21134519057895523	7.03463257203712E-5
108-109	0.12861170450305864	2.3586709212124462E-4	3.724217244019652E-5	1.6552076640087343E-5	0.21188727108891808	7.03463257203712E-5
110-111	0.12865101568507886	2.3586709212124462E-4	3.724217244019652E-5	1.6552076640087343E-5	0.21243348961804098	7.03463257203712E-5
112-113	0.1286613607329789	2.3586709212124462E-4	3.724217244019652E-5	1.6552076640087343E-5	0.21307074456868436	7.03463257203712E-5
114-115	0.12869239587667908	2.4000511128126646E-4	3.724217244019652E-5	1.6552076640087343E-5	0.2136355841840273	7.241533530038212E-5
116-117	0.1287172239916392	2.441431304412883E-4	3.9311182020207436E-5	1.6552076640087343E-5	0.21414869855987	7.655335446040396E-5
118-119	0.1287172239916392	2.4621214002129924E-4	4.138019160021836E-5	1.6552076640087343E-5	0.21464526085907265	8.06913736204258E-5
120-121	0.12872136201079923	2.482811496013101E-4	4.138019160021836E-5	1.6552076640087343E-5	0.2152080314648356	8.276038320043671E-5
122-123	0.12872343102037925	2.482811496013101E-4	4.138019160021836E-5	1.6552076640087343E-5	0.21570459376403822	8.689840236045854E-5
124-125	0.1287317070586993	2.482811496013101E-4	4.138019160021836E-5	2.069009580010918E-5	0.2162818474368613	8.896741194046946E-5
126-127	0.12875032814491938	2.52419168761332E-4	4.138019160021836E-5	2.2759105380120097E-5	0.2169480685216248	9.310543110049131E-5
128-129	0.12876067319281942	2.52419168761332E-4	4.138019160021836E-5	2.4828114960131014E-5	0.21756463337646803	9.517444068050222E-5
130-131	0.1287730872502995	2.689712454014193E-4	4.138019160021836E-5	2.4828114960131014E-5	0.2181646461546712	9.517444068050222E-5
132-133	0.12880619140357968	2.689712454014193E-4	4.138019160021836E-5	2.4828114960131014E-5	0.2188101771436346	9.517444068050222E-5
134-135	0.12881653645147975	2.7104025498143024E-4	4.138019160021836E-5	2.6897124540141932E-5	0.21940398289309776	9.517444068050222E-5
136-137	0.12882895050895982	2.77247283721463E-4	4.344920118022928E-5	2.896613412015285E-5	0.2199253733072605	9.517444068050222E-5
138-139	0.12883308852811984	2.7931629330147387E-4	4.551821076024019E-5	2.896613412015285E-5	0.22051711004714364	9.517444068050222E-5
>>END_MODULE
