##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.2d.R.1.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24185941
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	70-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.41784171225755	36.0	34.0	38.0	31.0	39.0
2	34.74561866333834	35.0	34.0	38.0	31.0	39.0
3	34.85463571584831	35.0	34.0	38.0	31.0	39.0
4	34.84388959685298	36.0	34.0	38.0	31.0	39.0
5	34.70879743732113	35.0	34.0	37.0	31.0	39.0
6	34.6996546878205	35.0	34.0	37.0	31.0	39.0
7	34.72002408341275	35.0	34.0	37.0	32.0	39.0
8	34.8355528114453	35.0	34.0	37.0	31.0	39.0
9	34.79656007595487	35.0	34.0	37.0	31.0	39.0
10-14	34.774486037156876	35.0	34.0	37.0	31.0	39.0
15-19	34.79352707426186	35.0	34.0	37.0	31.0	39.0
20-24	34.77373110270963	35.0	34.0	37.0	31.0	39.0
25-29	34.77000938685826	35.0	34.0	37.0	31.0	39.0
30-34	34.749174067694945	35.0	34.0	37.0	31.0	39.0
35-39	34.739659647726754	35.0	34.0	37.0	31.0	39.0
40-44	34.69124759710611	35.0	34.0	37.0	31.0	39.0
45-49	34.59128870776622	35.0	34.0	37.0	30.8	39.0
50-54	34.560052445344176	35.0	34.0	37.0	30.0	39.0
55-59	34.54605176618929	35.0	34.0	37.0	30.0	39.0
60-64	34.516939398801966	35.0	34.0	37.0	30.0	39.0
65-69	34.47844874011724	35.0	34.0	37.0	30.0	39.0
70-74	34.47935620443809	35.0	34.0	37.0	30.0	39.0
75-79	34.46107475262447	35.0	34.0	37.0	30.0	39.0
80-84	34.44481092889474	35.0	34.0	37.0	30.0	39.0
85-89	34.4233554029983	35.0	34.0	37.0	30.0	39.0
90-94	34.39788949586858	35.0	34.0	37.0	30.0	39.0
95-99	34.380590682072345	35.0	34.0	37.0	30.0	39.0
100-104	34.33872093982396	35.0	34.0	37.0	30.0	39.0
105-109	34.31194878746426	35.0	34.0	37.0	30.0	39.0
110-114	34.27539587684381	35.0	34.0	37.0	30.0	39.0
115-119	34.236116718924464	35.0	34.0	37.0	30.0	39.0
120-124	34.19492106564884	35.0	34.0	37.0	29.2	39.0
125-129	34.18066704981136	35.0	34.0	37.0	29.0	39.0
130-134	34.12581618562735	35.0	34.0	37.0	29.0	39.0
135-139	34.09061019550421	35.0	34.0	37.0	29.0	39.0
140-144	34.043368211963276	35.0	34.0	37.0	29.0	39.0
145-149	34.07912586044388	35.0	34.0	37.0	29.2	39.0
150	34.97575925062365	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	9.0
22	232.0
23	2605.0
24	18805.0
25	79556.0
26	209788.0
27	389246.0
28	580870.0
29	763541.0
30	958614.0
31	1202552.0
32	1541057.0
33	2021598.0
34	2774479.0
35	4265251.0
36	8222830.0
37	1154898.0
38	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.81031228017963	10.58059308091424	15.656938880318943	37.95215575858719
2	22.461210382356295	12.034724397822814	36.41029837219617	29.093766847624725
3	21.5872639474923	16.61529868023831	27.96541981945509	33.8320175528143
4	26.64688877079448	24.298154757211456	22.304710057995305	26.750246413998756
5	26.187344007994728	30.13125190322392	24.47522909492645	19.206174993854898
6	19.74797417374909	36.10669325402112	25.703638321348265	18.441694250881525
7	16.158389099911467	25.23285850634162	39.96840394655868	18.640348447188224
8	18.631936493309084	24.579226330157987	31.50061581273471	25.288221363798215
9	19.57582443907778	23.215411737957222	32.74180206185175	24.466961761113247
10-14	20.30516649333155	29.14047675766997	27.18891865682301	23.36543809217547
15-19	20.504319946674386	27.8013747216126	27.871534606997724	23.822770724715298
20-24	20.641718275199676	28.001869817438106	27.584724955301006	23.771686952061216
25-29	20.630243477857903	28.181156729658014	27.4631111983759	23.72548859410818
30-34	20.596963717525902	27.89330242605896	27.517870335317795	23.99186352109734
35-39	20.683967737863266	27.87016071215372	27.529605608128005	23.91626594185501
40-44	20.711551908498645	27.95972097237819	27.459943965879702	23.868783153243466
45-49	20.80608984252957	27.745753149492113	27.41568975999553	24.032467247982787
50-54	20.91948077095237	27.729474466623135	27.498908910620873	23.852135851803624
55-59	20.920073636255683	27.77040700387895	27.420384230290757	23.889135129574605
60-64	20.865963441334852	27.689761643708522	27.403243394682868	24.04103152027376
65-69	20.96267226871022	27.631721435102452	27.47580503510858	23.929801261078744
70-74	21.08311929875741	27.568599936314936	27.457174637420202	23.891106127507452
75-79	21.085916338206108	27.481945126441694	27.46051777242809	23.971620762924115
80-84	21.141787844874067	27.483688208160817	27.42183070057299	23.95269324639213
85-89	21.114675016350233	27.47400337616676	27.491349853372775	23.919971754110225
90-94	21.10438288898745	27.39409004049516	27.436948983105868	24.06457808741152
95-99	21.18827620550767	27.300473063247576	27.563472681213725	23.94777805003103
100-104	21.23565465914992	27.376291735090923	27.41068554904032	23.97736805671883
105-109	21.20868764447571	27.213316060383896	27.524915838695325	24.053080456445066
110-114	21.29560260787667	27.195088739464556	27.558481227754932	23.950827424903835
115-119	21.335329901957387	27.227958869599185	27.526251706342098	23.910459522101327
120-124	21.30135801547541	27.16232479616173	27.51359271205719	24.022724476305676
125-129	21.328757980889	27.078303446981916	27.572646697915857	24.020291874213225
130-134	21.408946417844987	27.192912967613147	27.46640527315593	23.931735341385938
135-139	21.39973127179914	27.07440758793192	27.559304639935622	23.966556500333315
140-144	21.449102054019885	26.997670229990327	27.676081717344957	23.877145998644828
145-149	21.04827914844894	27.049007529894283	27.843749825808356	24.058963495848424
150	20.966861882734463	25.742952829854183	28.52089273122047	24.769292556190884
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	1.5
9	2.0
10	3.0
11	5.5
12	8.5
13	16.0
14	32.0
15	74.5
16	153.5
17	304.0
18	592.0
19	1093.0
20	2073.5
21	3611.0
22	5711.5
23	9032.0
24	14110.0
25	21678.0
26	32020.0
27	45125.5
28	61524.5
29	85799.0
30	116333.5
31	148073.5
32	178661.0
33	212908.5
34	250979.0
35	297004.5
36	369511.5
37	468012.0
38	586716.5
39	734675.5
40	904072.0
41	1072662.5
42	1246150.0
43	1425861.0
44	1574690.0
45	1667008.5
46	1694723.0
47	1659596.0
48	1574412.5
49	1447871.5
50	1283597.0
51	1101396.0
52	919404.5
53	739802.0
54	573609.0
55	433515.0
56	326445.0
57	246294.5
58	184217.5
59	134721.5
60	94924.0
61	68562.5
62	49689.5
63	32890.5
64	21794.5
65	16563.5
66	15762.5
67	11879.5
68	5968.0
69	3724.0
70	3641.5
71	2656.5
72	1211.0
73	428.0
74	166.0
75	95.5
76	53.5
77	30.5
78	12.5
79	1.0
80	1.0
81	0.5
82	0.0
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001695199703001012
3	0.16096127911665706
4	7.442340159516638E-5
5	2.4807800531722124E-5
6	2.8942433953675814E-5
7	3.721170079758319E-5
8	7.028876817321269E-5
9	4.961560106344425E-5
10-14	1.4636602313716055E-4
15-19	1.678661169313197E-4
20-24	2.0838552446646587E-4
25-29	1.546352899810679E-4
30-34	2.025970376757307E-4
35-39	6.863491480443122E-5
40-44	1.4223138971520687E-4
45-49	1.7117382366888266E-4
50-54	8.848115522980891E-5
55-59	1.1990436923665695E-4
60-64	1.9432777083182334E-4
65-69	0.06536359284098146
70-74	2.0755860636386265E-4
75-79	1.2486594689634857E-4
80-84	9.592353346375571E-5
85-89	8.269277237798109E-5
90-94	1.4471249885160295E-4
95-99	9.17892574219305E-5
100-104	7.525088272179698E-5
105-109	9.840540410063423E-5
110-114	4.630865943732812E-5
115-119	4.6308983028105034E-5
120-124	9.592662235259165E-5
125-129	9.923589026071848E-5
130-134	3.638733955777921E-5
135-139	3.804332148498897E-5
140-144	5.294929036533719E-5
145-149	3.204492793344478E-5
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
70-71	1.0
72-73	1.0
74-75	1.0
76-77	0.0
78-79	2.0
80-81	3.0
82-83	9.0
84-85	8.0
86-87	13.0
88-89	7.0
90-91	10.0
92-93	14.0
94-95	24.0
96-97	22.0
98-99	30.0
100-101	34.0
102-103	33.0
104-105	39.0
106-107	54.0
108-109	44.0
110-111	45.0
112-113	64.0
114-115	65.0
116-117	85.0
118-119	81.0
120-121	89.0
122-123	123.0
124-125	148.0
126-127	193.0
128-129	195.0
130-131	255.0
132-133	311.0
134-135	469.0
136-137	809.0
138-139	2452.0
140-141	5306.0
142-143	9029.0
144-145	74450.0
146-147	774117.0
148-149	1403297.0
150-151	2.1914009E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.29820226862275
#Duplication Level	Percentage of total
1	40.25472606020896
2	10.084076194632242
3	6.1991782611327375
4	4.220583481781345
5	3.3165543744910138
6	2.5770117541398068
7	2.193294432236176
8	1.771724993775263
9	1.5149015690365362
>10	18.000993901213405
>50	4.444085734451517
>100	4.6603709793391
>500	0.4315431617893962
>1k	0.3309551017724809
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	2.4807800531722127E-5	0.0	8.269266843907376E-6	1.7365460372205488E-4	1.6538533687814753E-5
2	0.0	1.4884680319033277E-4	0.0	1.2403900265861064E-5	0.001773757738018132	2.067316710976844E-5
3	8.269266843907376E-6	1.6125070345619384E-4	0.0	1.2403900265861064E-5	0.002704050257957712	7.442340159516638E-5
4	2.067316710976844E-5	1.7365460372205488E-4	0.0	1.6538533687814753E-5	0.003936171017699911	9.923120212688851E-5
5	2.4807800531722127E-5	1.7365460372205488E-4	0.0	1.6538533687814753E-5	0.005077329842159129	1.281736360805643E-4
6	4.548096764149057E-5	1.7365460372205488E-4	0.0	1.6538533687814753E-5	0.006185411599242717	1.364429029244717E-4
7	5.788486790735163E-5	2.1086630451963809E-4	0.0	1.6538533687814753E-5	0.00738858992503124	1.4057753634642538E-4
8	6.615413475125901E-5	2.1086630451963809E-4	0.0	1.6538533687814753E-5	0.009253309598332354	1.4884680319033277E-4
9	7.028876817321269E-5	2.1086630451963809E-4	0.0	1.6538533687814753E-5	0.012168226160809703	1.5298143661228645E-4
10-11	1.0543315225981903E-4	2.2533752149647598E-4	0.0	2.067316710976844E-5	0.016224301547746272	2.1086630451963809E-4
12-13	1.6331802016717067E-4	2.3153947162940652E-4	0.0	2.687511724269897E-5	0.019794557507603283	2.3774142176233705E-4
14-15	1.881258206988928E-4	2.3774142176233705E-4	0.0	2.8942433953675814E-5	0.023838228994273986	2.501453220281981E-4
16-17	2.0879898780866123E-4	2.4187605518429076E-4	0.0	2.8942433953675814E-5	0.02815271897008266	2.6461653900503604E-4
18-19	2.3774142176233705E-4	2.4807800531722126E-4	0.0	2.8942433953675814E-5	0.031824273448777536	2.666838557160129E-4
20-21	2.852897061148044E-4	2.9149165624773503E-4	0.0	3.100975066465266E-5	0.03514438408660635	2.770204392708971E-4
22-23	3.5971310770997083E-4	3.059628732245729E-4	0.0	3.927901750856003E-5	0.03855959129314009	2.894243395367582E-4
24-25	3.989921252185309E-4	3.0803018993554975E-4	0.0	4.134633421953688E-5	0.04247508914373023	2.956262896696887E-4
26-27	4.5687699312588247E-4	3.100975066465266E-4	0.0	4.341365093051373E-5	0.046365779193788655	3.1009750664652655E-4
28-29	4.940886939234657E-4	3.100975066465266E-4	0.0	5.9952184618328476E-5	0.05050247993245332	3.204340902014108E-4
30-31	5.581755119637478E-4	3.100975066465266E-4	0.0	6.822145146223585E-5	0.05450687240161546	3.535111575770403E-4
32-33	5.912525793393774E-4	3.1423214006848026E-4	0.0	7.442340159516638E-5	0.058159821029911546	3.927901750856003E-4
34-35	6.284642801369605E-4	3.1836677349043397E-4	0.0	7.442340159516638E-5	0.06149026825129525	4.051940753514614E-4
36-37	6.367335469808679E-4	3.204340902014108E-4	0.0	7.442340159516638E-5	0.06479384035543624	4.279345591722067E-4
38-39	6.636086642235669E-4	3.3077067375629504E-4	0.0	7.855803501712007E-5	0.06848193336781894	4.4860772628197516E-4
40-41	7.152915819979881E-4	3.3077067375629504E-4	0.0	8.062535172809692E-5	0.07135757091278772	4.630789432588131E-4
42-43	7.235608488418954E-4	3.3490530717824875E-4	0.0	9.096193528298113E-5	0.07439652647792369	4.7341552681369725E-4
44-45	7.400993825297101E-4	3.431745740221561E-4	0.0	9.096193528298113E-5	0.07811976387439298	4.796174769466278E-4
46-47	7.711091331943627E-4	3.4730920744410976E-4	0.0	9.509656870493483E-5	0.08176237591913418	4.8375211036858146E-4
48-49	8.021188838590155E-4	3.5144384086606347E-4	0.0	1.0129851883786535E-4	0.0851072943574947	4.940886939234657E-4
50-51	8.248593676797608E-4	3.5971310770997083E-4	0.0	1.033658355488422E-4	0.08886360882133963	5.044252774783499E-4
52-53	8.992827692749271E-4	3.721170079758319E-4	0.0	1.033658355488422E-4	0.09258064426767601	5.085599109003037E-4
54-55	9.509656870493482E-4	3.721170079758319E-4	0.0	1.033658355488422E-4	0.09554310911450582	5.085599109003037E-4
56-57	9.654369040261861E-4	3.762516413977856E-4	0.0	1.0750046897079588E-4	0.09932629869559345	5.230311278771416E-4
58-59	9.881773878469315E-4	3.989921252185309E-4	0.0	1.1576973581470326E-4	0.10301439170797613	5.292330780100721E-4
60-61	0.0010109178716676767	4.134633421953688E-4	0.0	1.1576973581470326E-4	0.10603060678929135	5.333677114320258E-4
62-63	0.0010253890886445147	4.3000187588318353E-4	0.0	1.1576973581470326E-4	0.10912331258891271	5.333677114320258E-4
64-65	0.001033658355488422	4.3000187588318353E-4	0.0	1.1990436923665695E-4	0.11350602401618362	5.333677114320258E-4
66-67	0.0010522642058872135	4.5274235970392887E-4	0.0	1.2610631936958749E-4	0.11738224284926521	5.375023448539794E-4
68-69	0.0010812066398408894	4.713482101027204E-4	0.0	1.281736360805643E-4	0.11996225410456429	5.56108195252771E-4
70-71	0.0011411588244592177	4.8375211036858146E-4	0.0	1.281736360805643E-4	0.12240788977364991	5.68512095518632E-4
72-73	0.0011763032085458243	4.8788674379053517E-4	0.0	1.3230826950251802E-4	0.12507886296423198	5.726467289405858E-4
74-75	0.0011949090589446158	4.982233273454193E-4	0.0	1.5711607003424013E-4	0.12792969270866905	5.747140456515626E-4
76-77	0.0012217841761873148	5.044252774783499E-4	0.0	1.653853368781475E-4	0.13030090497615948	5.747140456515626E-4
78-79	0.0012486592934300138	5.126945443222573E-4	0.0	1.8192387056596227E-4	0.13265557870996214	5.747140456515626E-4
80-81	0.0012879383109385737	5.126945443222573E-4	0.0	1.8605850398791595E-4	0.13514669534668922	5.767813623625394E-4
82-83	0.0013416885454239718	5.230311278771416E-4	0.0	1.8605850398791595E-4	0.1374517534794284	5.788486790735164E-4
84-85	0.0014078426801752307	5.333677114320258E-4	0.0	1.8605850398791595E-4	0.13957902237502356	5.788486790735164E-4
86-87	0.0014615929146606288	5.354350281430026E-4	0.0	1.8605850398791595E-4	0.14149335764938814	5.850506292064469E-4
88-89	0.001496737298747235	5.395696615649562E-4	0.0	1.8605850398791595E-4	0.14356274167707594	5.871179459174237E-4
90-91	0.0015091411990130962	5.499062451198405E-4	0.0	1.8605850398791595E-4	0.14605385831380305	5.933198960503543E-4
92-93	0.0015442855830997025	5.519735618308174E-4	0.0	1.881258206988928E-4	0.14853463836697528	6.015891628942616E-4
94-95	0.0015959685008741236	5.56108195252771E-4	0.0	1.9226045412084648E-4	0.1512965734928403	6.036564796052384E-4
96-97	0.0016869304361571047	5.643774620966784E-4	0.0	1.9432777083182334E-4	0.1534858618897648	6.036564796052384E-4
98-99	0.0017551518876193404	5.809159957844932E-4	0.0	1.9432777083182334E-4	0.15536298546333177	6.036564796052384E-4
100-101	0.0018192387056596227	5.8298331249547E-4	0.0	1.9432777083182334E-4	0.1572897246379622	6.036564796052384E-4
102-103	0.0018461138229023217	5.871179459174237E-4	0.0	1.9432777083182334E-4	0.15888982777225827	6.036564796052384E-4
104-105	0.0018771235735669744	5.933198960503543E-4	0.0	1.963950875428002E-4	0.16047132505615555	6.057237963162153E-4
106-107	0.0019288064913413954	5.995218461832848E-4	0.0	1.9846240425377702E-4	0.1619659950381918	6.098584297381689E-4
108-109	0.002036306960312191	6.036564796052384E-4	0.0	2.025970376757307E-4	0.16355989622235495	6.119257464491458E-4
110-111	0.0021148649953293113	6.098584297381689E-4	0.0	2.025970376757307E-4	0.1650028832866168	6.119257464491458E-4
112-113	0.0021996249804793616	6.181276965820763E-4	0.0	2.025970376757307E-4	0.16650995716891892	6.119257464491458E-4
114-115	0.0022843849656294128	6.243296467150069E-4	0.0	2.1086630451963806E-4	0.16818861833823212	6.119257464491458E-4
116-117	0.0023505391003806712	6.305315968479373E-4	0.0	2.2740483820745284E-4	0.16972876928790986	6.119257464491458E-4
118-119	0.0024084239682880234	6.532720806686827E-4	0.0	2.3360678834038337E-4	0.17106218856648991	6.119257464491458E-4
120-121	0.0025035205369929583	6.574067140906364E-4	0.0	2.398087384733139E-4	0.17249483904719687	6.119257464491458E-4
122-123	0.002623424906229615	6.574067140906364E-4	0.0	2.4394337189526758E-4	0.1737248924902281	6.119257464491458E-4
124-125	0.002730925375200411	6.594740308016132E-4	0.0	2.460106886062444E-4	0.1750913888361838	6.119257464491458E-4
126-127	0.002778473659552878	6.615413475125901E-4	0.0	2.4807800531722126E-4	0.17635245202987965	6.119257464491458E-4
128-129	0.0028549643778590216	6.615413475125901E-4	0.0	2.563472721611286E-4	0.17758457278962186	6.160603798710995E-4
130-131	0.002902512662211489	6.615413475125901E-4	0.0	2.625492222940592E-4	0.1788766457339824	6.181276965820763E-4
132-133	0.0029583302134078638	6.656759809345438E-4	0.0	2.6461653900503604E-4	0.18009636259345874	6.222623300040301E-4
134-135	0.0030596287322457293	6.718779310674743E-4	0.0	2.6461653900503604E-4	0.18107833803117274	6.243296467150069E-4
136-137	0.0032126101688580155	6.739452477784511E-4	0.0	2.6461653900503604E-4	0.18211613102008312	6.243296467150069E-4
138-139	0.003233283335967784	6.76012564489428E-4	0.0	2.6461653900503604E-4	0.1832324820440106	6.243296467150069E-4
>>END_MODULE
