##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.2d.R.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24185941
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	60-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.787882844831216	36.0	35.0	38.0	32.0	39.0
2	35.15489143052156	35.0	34.0	37.0	32.0	39.0
3	35.292860922798084	35.0	34.0	37.0	33.0	39.0
4	35.344199549647456	36.0	34.0	37.0	33.0	39.0
5	35.256370343415625	35.0	34.0	37.0	32.0	39.0
6	35.221762221283846	35.0	34.0	37.0	33.0	39.0
7	35.262451562252636	35.0	34.0	37.0	33.0	39.0
8	35.398742600091516	36.0	34.0	38.0	33.0	39.0
9	35.369152723890295	36.0	34.0	38.0	33.0	39.0
10-14	35.328794790328814	35.4	34.0	37.4	32.4	39.0
15-19	35.299740150693324	35.0	34.0	37.0	32.2	39.0
20-24	35.264883570169964	35.0	34.0	37.0	32.0	39.0
25-29	35.25432905008741	35.0	34.0	37.0	32.0	39.0
30-34	35.23622277090645	35.0	34.0	37.0	32.0	39.0
35-39	35.21004768017916	35.0	34.0	37.0	32.0	39.0
40-44	35.18785627567685	35.0	34.0	37.0	32.0	39.0
45-49	35.15871710759569	35.0	34.0	37.0	32.0	39.0
50-54	35.13355671379501	35.0	34.0	37.0	32.0	39.0
55-59	35.1024899382662	35.0	34.0	37.0	32.0	39.0
60-64	35.060749849638746	35.0	34.0	37.0	32.0	39.0
65-69	35.02378077880792	35.0	34.0	37.0	32.0	39.0
70-74	35.0002199024322	35.0	34.0	37.0	32.0	39.0
75-79	34.949383761284494	35.0	34.0	37.0	31.0	39.0
80-84	34.87103124028686	35.0	34.0	37.0	31.0	39.0
85-89	34.85520692897301	35.0	34.0	37.0	31.0	39.0
90-94	34.80390168539841	35.0	34.0	37.0	31.0	39.0
95-99	34.73835622244233	35.0	34.0	37.0	31.0	39.0
100-104	34.669100053363024	35.0	34.0	37.0	30.4	39.0
105-109	34.60999647885706	35.0	34.0	37.0	30.0	39.0
110-114	34.54351047895044	35.0	34.0	37.0	30.0	39.0
115-119	34.484711869827606	35.0	34.0	37.0	30.0	39.0
120-124	34.4097213046084	35.0	34.0	37.0	30.0	39.0
125-129	34.32807417125359	35.0	34.0	37.0	29.6	39.0
130-134	34.25094693864455	35.0	34.0	37.0	29.0	39.0
135-139	34.162496069453304	35.0	34.0	37.0	29.0	39.0
140-144	34.06618898166865	35.0	34.0	37.0	29.0	39.0
145-149	34.035308641792106	35.0	34.0	37.0	28.8	39.0
150	34.896117486486084	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	17.0
21	214.0
22	2069.0
23	10743.0
24	37076.0
25	89794.0
26	177844.0
27	299466.0
28	435327.0
29	570674.0
30	724926.0
31	936655.0
32	1235229.0
33	1694788.0
34	2495167.0
35	4140661.0
36	9879225.0
37	1456060.0
38	6.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.8776953520229	19.142678798397796	16.374909704774357	31.60471614480495
2	23.73639710772469	26.03262366347458	33.258404128249545	16.972575100551186
3	19.671713414003612	29.707642964977047	30.12434785977523	20.496295761244106
4	23.243834093533923	34.176272074756156	22.543286614318625	20.036607217391296
5	24.84077423326221	35.171461800886725	22.758622457567395	17.22914150828368
6	18.767514565589984	38.71081964518147	23.24024936635709	19.28141642287145
7	20.05857039012871	20.60504075487491	38.2567707413162	21.079618113680173
8	21.47775023514694	23.30136751760041	28.45185556352759	26.769026683725063
9	23.380979884140128	23.328358404578925	28.761184028357633	24.529477682923314
10-14	22.964903453622085	29.142162382683395	25.781353721155607	22.111580442538912
15-19	22.789724185548323	28.28081014735635	27.241939664385516	21.687526002709806
20-24	22.908244152531097	28.30693596807604	27.149637205545524	21.63518267384734
25-29	23.035181471748402	28.246418032690972	27.06008337653681	21.658317119023817
30-34	23.04236415692902	28.0382078166816	27.22948344246767	21.689944583921708
35-39	23.14641551469922	28.003395857122122	27.198346345093622	21.65184228308504
40-44	23.292474748036472	27.763223270907673	27.309250444297373	21.635051536758482
45-49	23.320269407752214	27.589236242658494	27.373493551481005	21.71700079810829
50-54	23.408685622919293	27.566422618271048	27.319723095656656	21.705168663153003
55-59	23.5897466218081	27.43196884504101	27.213690796649175	21.764593736501713
60-64	23.516980915639103	27.415644418462037	27.25607567464528	21.81129899125358
65-69	23.612866975466517	27.33342430631462	27.213826700175286	21.83988201804358
70-74	23.68268412755375	27.29293158443638	27.113499137138703	21.910885150871167
75-79	23.62606970960699	27.341207259030103	27.17152186221251	21.861201169150394
80-84	23.7485197123243	27.388952307378702	27.026648963826556	21.835879016470454
85-89	23.788361284021004	27.314303534530815	27.074957517584945	21.822377663863232
90-94	23.72791179788555	27.251503754296934	27.118447633550407	21.902136814267106
95-99	23.802727236356354	27.365423119929112	27.108566406175765	21.723283237538766
100-104	23.972252236699344	27.33721470615941	26.916916190362887	21.773616866778355
105-109	23.75897138905596	27.48399194157618	27.0096130635052	21.747423605862657
110-114	23.80706176063365	27.475905028323822	26.928609978446804	21.788423232595726
115-119	23.881048751000638	27.47655522387146	26.940965381964322	21.70143064316358
120-124	23.84665802244487	27.377878864116834	27.11974811008961	21.655715003348686
125-129	23.826256949468856	27.467892392965677	27.06432801025696	21.64152264730851
130-134	23.886671210477285	27.360153693430618	27.144009899830902	21.609165196261195
135-139	23.796569114152238	27.382255084723646	27.235531936040523	21.585643865083597
140-144	23.83142815324732	27.479839768949045	27.209319845031544	21.479412232772095
145-149	23.526285917125524	27.464602969545858	27.27071015960251	21.73840095372611
150	23.63314532608484	26.3071728291097	27.74052009953165	22.31916174527381
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	9.5
3	29.0
4	42.5
5	44.0
6	37.5
7	23.0
8	14.0
9	15.0
10	14.0
11	10.5
12	9.0
13	14.0
14	28.5
15	42.0
16	75.5
17	148.5
18	225.5
19	357.5
20	580.0
21	890.0
22	1444.5
23	2548.5
24	4171.0
25	6768.0
26	10424.0
27	16718.5
28	28423.0
29	43397.5
30	57444.0
31	74989.5
32	100591.5
33	134807.5
34	181824.5
35	252119.5
36	354224.0
37	477258.0
38	622268.0
39	810022.5
40	1029424.0
41	1233458.0
42	1412415.5
43	1576329.0
44	1695010.5
45	1745727.0
46	1743500.0
47	1688501.0
48	1583017.5
49	1437931.0
50	1251357.0
51	1051429.5
52	859114.5
53	684287.5
54	535928.5
55	407214.0
56	301764.0
57	223573.5
58	163817.0
59	116736.0
60	81494.0
61	55933.5
62	38456.5
63	26115.0
64	17227.5
65	11734.5
66	8277.5
67	6068.0
68	5243.5
69	4082.5
70	2618.0
71	2085.5
72	1301.0
73	946.0
74	833.0
75	546.5
76	346.0
77	121.0
78	31.0
79	5.0
80	1.0
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	8.269266843907376E-7
20-24	0.033224260325451054
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	1.6538533687814751E-6
55-59	0.0
60-64	0.0
65-69	8.269268621807886E-7
70-74	0.0
75-79	7.60774361460785E-4
80-84	0.06479833935649444
85-89	1.6538729532670991E-6
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	3.989306473636321E-4
145-149	0.03543370967062132
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
60-61	2.0
62-63	0.0
64-65	2.0
66-67	2.0
68-69	2.0
70-71	5.0
72-73	11.0
74-75	14.0
76-77	38.0
78-79	23.0
80-81	35.0
82-83	45.0
84-85	68.0
86-87	72.0
88-89	85.0
90-91	101.0
92-93	106.0
94-95	112.0
96-97	111.0
98-99	153.0
100-101	143.0
102-103	154.0
104-105	182.0
106-107	191.0
108-109	194.0
110-111	203.0
112-113	220.0
114-115	232.0
116-117	229.0
118-119	243.0
120-121	331.0
122-123	350.0
124-125	375.0
126-127	460.0
128-129	488.0
130-131	602.0
132-133	874.0
134-135	1157.0
136-137	1665.0
138-139	3593.0
140-141	7446.0
142-143	11924.0
144-145	74935.0
146-147	843958.0
148-149	1159415.0
150-151	2.207539E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.917511239927286
#Duplication Level	Percentage of total
1	28.879823763283248
2	10.287425103662816
3	6.825516622595205
4	5.023051999561668
5	3.7411205359125974
6	3.062048074727659
7	2.4617599880621346
8	2.138213944672744
9	1.8466654167695356
>10	21.232537092797703
>50	5.4567546456027625
>100	6.735054325068929
>500	1.1841918514942795
>1k	1.0257127183443042
>5k	0.054092823631197606
>10k+	0.04603109381310933
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.134633421953688E-6	8.269266843907376E-6	4.134633421953688E-6	0.0	0.001496737298747235	0.0
2	2.067316710976844E-5	1.2403900265861064E-5	4.134633421953688E-6	0.0	0.006028295529208477	0.0
3	5.375023448539794E-5	1.2403900265861064E-5	4.134633421953688E-6	4.134633421953688E-6	0.00785993813513396	0.0
4	1.033658355488422E-4	1.2403900265861064E-5	4.134633421953688E-6	4.134633421953688E-6	0.009199559363846956	0.0
5	1.0750046897079588E-4	1.2403900265861064E-5	4.134633421953688E-6	4.134633421953688E-6	0.01088648980000406	0.0
6	1.2403900265861063E-4	1.6538533687814753E-5	4.134633421953688E-6	4.134633421953688E-6	0.012565150969317257	0.0
7	1.3230826950251802E-4	3.3077067375629505E-5	4.134633421953688E-6	4.134633421953688E-6	0.014566313545542843	0.0
8	2.067316710976844E-4	3.3077067375629505E-5	4.134633421953688E-6	8.269266843907376E-6	0.02023489596704135	0.0
9	2.1086630451963809E-4	4.134633421953688E-5	4.134633421953688E-6	8.269266843907376E-6	0.026209441261764426	0.0
10-11	2.356741050513602E-4	4.341365093051373E-5	4.134633421953688E-6	8.269266843907376E-6	0.032769037185693954	0.0
12-13	2.666838557160129E-4	4.9615601063444254E-5	4.134633421953688E-6	8.269266843907376E-6	0.03884901563267685	0.0
14-15	2.873570228257813E-4	6.615413475125901E-5	4.134633421953688E-6	8.269266843907376E-6	0.047329148781103864	4.134633421953688E-6
16-17	3.059628732245729E-4	6.615413475125901E-5	4.134633421953688E-6	8.269266843907376E-6	0.05304734680366582	4.134633421953688E-6
18-19	3.5971310770997083E-4	6.615413475125901E-5	4.134633421953688E-6	1.033658355488422E-5	0.060818390320227775	4.134633421953688E-6
20-21	3.948574917965772E-4	7.649071830614323E-5	4.134633421953688E-6	1.2403900265861064E-5	0.06705548483724491	4.134633421953688E-6
22-23	4.3620382601611404E-4	9.509656870493483E-5	4.134633421953688E-6	1.2403900265861064E-5	0.07392517826782097	4.134633421953688E-6
24-25	4.89954060501512E-4	9.509656870493483E-5	4.134633421953688E-6	1.2403900265861064E-5	0.07904798907762159	4.134633421953688E-6
26-27	5.31300394721049E-4	1.033658355488422E-4	4.134633421953688E-6	1.2403900265861064E-5	0.08321776688366188	4.134633421953688E-6
28-29	6.346662302698911E-4	1.0750046897079588E-4	4.134633421953688E-6	1.2403900265861064E-5	0.08729865007113016	4.134633421953688E-6
30-31	7.979842504370618E-4	1.1163510239274958E-4	1.2403900265861064E-5	1.2403900265861064E-5	0.09265093303584922	4.134633421953688E-6
32-33	9.592349538932556E-4	1.1576973581470326E-4	1.6538533687814753E-5	1.2403900265861064E-5	0.09816653402073544	8.269266843907376E-6
34-35	0.001085341273262843	1.1576973581470326E-4	1.6538533687814753E-5	1.4471216976837909E-5	0.1013936154065703	8.269266843907376E-6
36-37	0.0011411588244592179	1.1576973581470326E-4	1.8605850398791596E-5	1.6538533687814753E-5	0.10477367822901743	8.269266843907376E-6
38-39	0.0012176495427653612	1.2197168594763378E-4	2.067316710976844E-5	1.6538533687814753E-5	0.10844523270771231	8.269266843907376E-6
40-41	0.001271399777250759	1.3024095279154117E-4	2.067316710976844E-5	1.6538533687814753E-5	0.11132707220281401	1.033658355488422E-5
42-43	0.0012900056276495507	1.4264485305740224E-4	2.067316710976844E-5	1.6538533687814753E-5	0.11421718096475965	1.2403900265861064E-5
44-45	0.0013168807448922495	1.6745265358912435E-4	2.067316710976844E-5	1.6538533687814753E-5	0.11654911421474154	1.4471216976837909E-5
46-47	0.0013726982960886244	1.9432777083182334E-4	2.067316710976844E-5	1.6538533687814753E-5	0.11899681720053812	1.6538533687814753E-5
48-49	0.0014223138971520685	2.1086630451963809E-4	2.067316710976844E-5	1.6538533687814753E-5	0.12138663531842735	1.6538533687814753E-5
50-51	0.001471929498215513	2.1706825465256862E-4	2.067316710976844E-5	1.6538533687814753E-5	0.12358005834877378	1.6538533687814753E-5
52-53	0.0015194777825679804	2.2327020478549913E-4	2.067316710976844E-5	1.6538533687814753E-5	0.1258458374640044	1.6538533687814753E-5
54-55	0.0015525548499436097	2.3153947162940652E-4	2.067316710976844E-5	1.6538533687814753E-5	0.12784079809009705	2.067316710976844E-5
56-57	0.0016228436181168226	2.460106886062444E-4	2.067316710976844E-5	1.8605850398791596E-5	0.1298543645665885	2.067316710976844E-5
58-59	0.0016807284860241743	2.5221263873917497E-4	2.2740483820745284E-5	2.067316710976844E-5	0.1316736032722481	2.067316710976844E-5
60-61	0.0017510172541973868	2.6048190558308233E-4	2.4807800531722127E-5	2.067316710976844E-5	0.13317860983783925	2.4807800531722127E-5
62-63	0.001813036755526692	2.7495312255992026E-4	2.4807800531722127E-5	2.067316710976844E-5	0.13526246508250392	3.3077067375629505E-5
64-65	0.001837844556058414	3.0389555651359605E-4	2.4807800531722127E-5	2.2740483820745284E-5	0.13708377110487452	3.721170079758319E-5
66-67	0.0019102006409426038	3.369726238892256E-4	2.4807800531722127E-5	2.4807800531722127E-5	0.13844820013411924	3.721170079758319E-5
68-69	0.001947412341740187	3.535111575770403E-4	2.4807800531722127E-5	2.4807800531722127E-5	0.13971753259465902	3.721170079758319E-5
70-71	0.0019784220924048395	3.5764579099899403E-4	2.4807800531722127E-5	2.4807800531722127E-5	0.1408710953193841	3.721170079758319E-5
72-73	0.0020218357433353534	3.8245359153071617E-4	2.4807800531722127E-5	2.4807800531722127E-5	0.14181379173958952	4.9615601063444254E-5
74-75	0.002038374277023168	3.927901750856004E-4	2.4807800531722127E-5	2.8942433953675814E-5	0.14288259447916457	5.375023448539794E-5
76-77	0.0020735186611097745	4.2173260903927617E-4	2.8942433953675814E-5	2.8942433953675814E-5	0.14415399425641534	5.788486790735163E-5
78-79	0.0021252015788841955	4.610116265478362E-4	2.8942433953675814E-5	2.8942433953675814E-5	0.14575203007400042	5.788486790735163E-5
80-81	0.0021417401125720104	4.816847936576046E-4	2.8942433953675814E-5	2.8942433953675814E-5	0.14717020933773053	5.788486790735163E-5
82-83	0.0021582786462598248	4.961560106344425E-4	2.8942433953675814E-5	2.8942433953675814E-5	0.14884887050704373	5.788486790735163E-5
84-85	0.002185153763502524	5.064925941893267E-4	2.8942433953675814E-5	2.8942433953675814E-5	0.15041382925725322	5.788486790735163E-5
86-87	0.0022099615640342463	5.126945443222573E-4	2.8942433953675814E-5	2.8942433953675814E-5	0.15225167381331162	5.788486790735163E-5
88-89	0.0022265000977220607	5.188964944551878E-4	3.100975066465266E-5	2.8942433953675814E-5	0.15397995058368827	6.615413475125901E-5
90-91	0.002247173264831829	5.333677114320258E-4	3.3077067375629505E-5	2.8942433953675814E-5	0.15488130066967418	6.615413475125901E-5
92-93	0.0022533752149647597	5.375023448539794E-4	3.3077067375629505E-5	2.8942433953675814E-5	0.1558984204914748	6.615413475125901E-5
94-95	0.0022699137486525745	5.416369782759331E-4	3.3077067375629505E-5	2.8942433953675814E-5	0.15675015497639724	6.615413475125901E-5
96-97	0.0022699137486525745	5.478389284088636E-4	3.3077067375629505E-5	2.8942433953675814E-5	0.15763496652869532	6.822145146223585E-5
98-99	0.00229265423247332	5.602428286747246E-4	3.3077067375629505E-5	2.8942433953675814E-5	0.15850117223059462	7.649071830614323E-5
100-101	0.0023133273995830884	5.623101453857015E-4	3.3077067375629505E-5	2.8942433953675814E-5	0.15929502184760974	7.855803501712007E-5
102-103	0.0023153947162940654	5.664447788076552E-4	4.341365093051373E-5	3.100975066465266E-5	0.16003925586356138	8.269266843907376E-5
104-105	0.0023153947162940654	5.788486790735162E-4	4.548096764149057E-5	3.3077067375629505E-5	0.16083930743070946	8.269266843907376E-5
106-107	0.002329865933270903	5.933198960503543E-4	4.754828435246741E-5	3.3077067375629505E-5	0.1615483970625745	8.269266843907376E-5
108-109	0.002342269833536764	6.222623300040301E-4	5.375023448539794E-5	3.3077067375629505E-5	0.16226575596128345	8.269266843907376E-5
110-111	0.0023588083672245793	6.325989135589143E-4	5.788486790735163E-5	3.3077067375629505E-5	0.16307614411198637	8.682730186102744E-5
112-113	0.0023712122674904397	6.532720806686827E-4	5.788486790735163E-5	3.3077067375629505E-5	0.16378523374385145	8.889461857200428E-5
114-115	0.0023774142176233703	6.594740308016132E-4	5.788486790735163E-5	3.3077067375629505E-5	0.16459148726113243	9.509656870493483E-5
116-117	0.0023815488510453243	6.63608664223567E-4	5.788486790735163E-5	3.721170079758319E-5	0.16552591441449394	1.0129851883786535E-4
118-119	0.0023815488510453243	6.698106143564974E-4	5.788486790735163E-5	3.721170079758319E-5	0.16627221574715656	1.033658355488422E-4
120-121	0.0023815488510453243	6.739452477784511E-4	5.788486790735163E-5	4.134633421953688E-5	0.16701231512968628	1.0543315225981904E-4
122-123	0.0023815488510453243	6.822145146223585E-4	5.788486790735163E-5	4.134633421953688E-5	0.167752414512216	1.0956778568177274E-4
124-125	0.002383616167756301	6.88416464755289E-4	6.201950132930532E-5	4.134633421953688E-5	0.16851732169527744	1.1576973581470326E-4
126-127	0.0023939527513111855	6.904837814662659E-4	6.201950132930532E-5	4.134633421953688E-5	0.16940213324757553	1.178370525256801E-4
128-129	0.0024146259184209535	6.966857315991964E-4	6.201950132930532E-5	4.134633421953688E-5	0.17027454089960775	1.1990436923665695E-4
130-131	0.0024352990855307224	7.111569485760344E-4	6.201950132930532E-5	4.134633421953688E-5	0.17116141976861682	1.2197168594763379E-4
132-133	0.002453904935929514	7.111569485760344E-4	6.201950132930532E-5	4.548096764149057E-5	0.1719304615851002	1.2610631936958749E-4
134-135	0.0024725107863283052	7.132242652870113E-4	6.201950132930532E-5	4.548096764149057E-5	0.17263955121696528	1.281736360805643E-4
136-137	0.00248284736988319	7.152915819979881E-4	6.201950132930532E-5	4.548096764149057E-5	0.1734396027841133	1.3024095279154117E-4
138-139	0.0024849146865941665	7.276954822638491E-4	6.201950132930532E-5	4.548096764149057E-5	0.1741362885157125	1.364429029244717E-4
>>END_MODULE
