##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.2d.R.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24134166
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	59-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.95929782698934	37.0	35.0	38.0	33.0	39.0
2	35.294179546125605	35.0	34.0	37.0	33.0	39.0
3	35.47388689544938	35.0	34.0	37.0	33.0	39.0
4	35.51657190059934	36.0	35.0	38.0	33.0	39.0
5	35.4228523993744	36.0	35.0	37.0	33.0	39.0
6	35.38815664895982	35.0	34.0	37.0	33.0	39.0
7	35.40459740767508	35.0	34.0	37.0	33.0	39.0
8	35.519162211778934	36.0	35.0	38.0	33.0	39.0
9	35.47147450630778	36.0	34.0	38.0	33.0	39.0
10-14	35.469004829087524	35.8	34.4	37.8	33.0	39.0
15-19	35.445398030327624	36.0	34.0	38.0	33.0	39.0
20-24	35.425380201661	36.0	34.0	38.0	33.0	39.0
25-29	35.40432775675778	36.0	34.0	38.0	33.0	39.0
30-34	35.38364230195483	36.0	34.0	38.0	33.0	39.0
35-39	35.354350127532896	36.0	34.0	38.0	33.0	39.0
40-44	35.33331415719938	36.0	34.0	37.8	32.8	39.0
45-49	35.3012862677749	36.0	34.0	38.0	32.0	39.0
50-54	35.27158799686718	36.0	34.0	37.2	32.0	39.0
55-59	35.24877774520984	36.0	34.0	37.2	32.0	39.0
60-64	35.20164564218401	35.6	34.0	37.0	32.0	39.0
65-69	35.17139779944686	35.6	34.0	37.0	32.0	39.0
70-74	35.1300633751964	35.2	34.0	37.0	32.0	39.0
75-79	35.085313097422876	35.0	34.0	37.0	32.0	39.0
80-84	35.040736931737456	35.0	34.0	37.0	32.0	39.0
85-89	34.9860335314479	35.0	34.0	37.0	32.0	39.0
90-94	34.92387457146251	35.0	34.0	37.0	31.0	39.0
95-99	34.85400611359969	35.0	34.0	37.0	31.0	39.0
100-104	34.80166332098038	35.0	34.0	37.0	31.0	39.0
105-109	34.73484232008134	35.0	34.0	37.0	31.0	39.0
110-114	34.658581430801725	35.0	34.0	37.0	30.6	39.0
115-119	34.58434240095197	35.0	34.0	37.0	30.0	39.0
120-124	34.50116797301687	35.0	34.0	37.0	30.0	39.0
125-129	34.4157238022146	35.0	34.0	37.0	30.0	39.0
130-134	34.325475626076276	35.0	34.0	37.0	29.8	39.0
135-139	34.24293540962989	35.0	34.0	37.0	29.0	39.0
140-144	34.111223172690664	35.0	34.0	37.0	29.0	39.0
145-149	34.097837865305806	35.0	34.0	37.0	29.0	39.0
150	34.92095968481026	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	139.0
22	1346.0
23	7780.0
24	28142.0
25	72083.0
26	146146.0
27	256580.0
28	382673.0
29	510314.0
30	661600.0
31	868969.0
32	1167993.0
33	1637682.0
34	2469483.0
35	4209767.0
36	1.02441E7
37	1469354.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.13648932389046	18.63473964669009	15.605179810232514	31.62359121918694
2	23.78442660914821	25.605790562640536	33.52477562307312	17.08500720513814
3	19.91854203704408	29.06966414335594	29.61026703802402	21.40152678157596
4	23.666900277390983	33.638672245811186	22.388045230152144	20.306382246645686
5	25.017578813371884	34.81184309414297	22.739252725783025	17.431325366702126
6	18.992191402014885	38.39999691723344	23.33912015024675	19.268691530504928
7	20.235814239447926	20.577802439910293	37.96374815686608	21.222635163775703
8	21.56263033908029	23.28038598889226	28.52357110662121	26.63341256540624
9	23.283717365663268	23.225198666487998	29.00109744832285	24.489986519525885
10-14	23.09842320633744	28.90411626405487	25.767820607515503	22.22963992209219
15-19	22.879658654871275	28.139106194927145	27.230068774698907	21.751166375502677
20-24	22.967622746938925	28.20217943309083	27.10231130423152	21.727886515738724
25-29	23.035035061911817	28.1658334495586	27.07237449183038	21.726756996699205
30-34	23.030527758862686	27.94978786505405	27.227947300934286	21.79173707514898
35-39	23.090532318373878	27.956062786673463	27.221519898388035	21.73188499656462
40-44	23.235496101253304	27.74151549301517	27.305888258164796	21.717100147566732
45-49	23.236949642262342	27.575700772092144	27.39793204372589	21.78941754191962
50-54	23.29588600658502	27.5951918123046	27.34521424937576	21.763707931734622
55-59	23.466914278014947	27.48886559816375	27.23619558460148	21.80802453921983
60-64	23.373565234181502	27.461821861249398	27.321645476443873	21.84296742812523
65-69	23.46599455492164	27.388835644399062	27.274180738676918	21.870989062002376
70-74	23.53739727800251	27.36472089198837	27.20056756767037	21.89731426233875
75-79	23.4613327870081	27.405038526031	27.271357953973318	21.862270732987582
80-84	23.559581395027763	27.458890521817413	27.14523189487883	21.83629618827599
85-89	23.606950214010897	27.37893026291986	27.18793354619646	21.826185976872786
90-94	23.51944046717061	27.334340221223734	27.27286634758274	21.873352964022914
95-99	23.594846848429057	27.432233000800245	27.27414109402885	21.698779056741856
100-104	23.747758697699094	27.401112837074855	27.095798888588373	21.75532957663767
105-109	23.544578981698567	27.519288558519506	27.208618600476335	21.72751385930559
110-114	23.562143764220163	27.495806857918836	27.155751581998178	21.786297795862822
115-119	23.638438340614115	27.494693261769786	27.180615349734012	21.68625304788209
120-124	23.612339136542445	27.39989405641327	27.368748853252384	21.619017953791897
125-129	23.566904105759424	27.509528158775698	27.324429325220617	21.59913841024426
130-134	23.634051413394065	27.40384967279904	27.38644635852994	21.57565255527695
135-139	23.54614593977708	27.397747265492423	27.493982499881035	21.562124294849465
140-144	23.570133339756644	27.528962708736426	27.469120749372028	21.431783202134902
145-149	23.281332112171107	27.597367944261375	27.472046019807618	21.649253923759908
150	23.35829472746761	26.431592059846505	27.912511741114738	22.297601471571156
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	0.5
5	0.0
6	0.5
7	0.5
8	2.0
9	4.5
10	5.5
11	5.5
12	12.5
13	15.0
14	18.5
15	35.0
16	59.5
17	108.5
18	188.5
19	325.0
20	527.0
21	838.0
22	1357.0
23	2406.5
24	4060.0
25	6618.0
26	10364.0
27	16542.0
28	27670.5
29	42155.0
30	57131.5
31	75272.5
32	100629.5
33	134202.0
34	180364.0
35	249348.5
36	348550.5
37	471338.5
38	616349.5
39	799139.5
40	1014407.5
41	1222385.0
42	1407920.5
43	1575048.5
44	1696414.5
45	1752379.5
46	1751565.5
47	1697430.0
48	1592752.5
49	1444869.5
50	1258445.5
51	1055642.0
52	861579.0
53	685770.5
54	533533.0
55	402606.0
56	296974.0
57	218944.5
58	159792.0
59	114769.0
60	79024.0
61	52549.0
62	35463.0
63	24071.5
64	16010.5
65	10747.5
66	7676.0
67	5819.0
68	5016.0
69	3693.0
70	1991.0
71	1255.5
72	703.0
73	472.5
74	395.0
75	276.0
76	191.0
77	79.5
78	20.5
79	2.5
80	0.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	8.287006893049463E-7
60-64	0.0
65-69	0.0
70-74	8.287014309900293E-7
75-79	0.0
80-84	8.287060871544889E-7
85-89	0.0
90-94	8.287234005789611E-7
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	1.6577449733255986E-6
130-134	0.0
135-139	0.0
140-144	8.29456364428223E-7
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
58-59	1.0
60-61	0.0
62-63	0.0
64-65	1.0
66-67	5.0
68-69	2.0
70-71	12.0
72-73	15.0
74-75	18.0
76-77	27.0
78-79	26.0
80-81	42.0
82-83	74.0
84-85	72.0
86-87	94.0
88-89	146.0
90-91	121.0
92-93	141.0
94-95	120.0
96-97	128.0
98-99	185.0
100-101	208.0
102-103	187.0
104-105	195.0
106-107	227.0
108-109	218.0
110-111	236.0
112-113	250.0
114-115	272.0
116-117	261.0
118-119	317.0
120-121	362.0
122-123	376.0
124-125	388.0
126-127	514.0
128-129	590.0
130-131	702.0
132-133	859.0
134-135	1156.0
136-137	1710.0
138-139	3561.0
140-141	7183.0
142-143	11459.0
144-145	72347.0
146-147	835687.0
148-149	1104627.0
150-151	2.2089044E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.28051031503468
#Duplication Level	Percentage of total
1	31.075039121464858
2	10.532598478292584
3	7.0046436364985585
4	5.00292815841804
5	3.8883914500748373
6	3.005580808833797
7	2.5751726233091943
8	2.1803585569956008
9	1.8532410985680672
>10	20.44717604925865
>50	5.020616013513133
>100	5.737271722332791
>500	0.9282284756309209
>1k	0.6539191363205508
>5k	0.09483467048840406
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	2.486102067914839E-5	0.0	0.0	7.043955859092044E-4	0.0
2	4.143503446524732E-6	2.9004524125673122E-5	0.0	0.0	0.004752598453163868	0.0
3	2.9004524125673122E-5	3.3148027572197856E-5	0.0	0.0	0.00624425969391277	0.0
4	8.287006893049464E-5	3.7291531018722584E-5	0.0	0.0	0.007557750286461111	0.0
5	8.701357237701937E-5	4.557853791177205E-5	0.0	0.0	0.008891958396242074	0.0
6	9.944408271659356E-5	4.557853791177205E-5	0.0	0.0	0.010325610588739632	0.0
7	1.1601809650269249E-4	5.3865544804821516E-5	0.0	0.0	0.012289631222392355	0.0
8	1.6159663441446453E-4	5.3865544804821516E-5	0.0	0.0	0.01727426586856161	0.0
9	1.6574013786098928E-4	5.3865544804821516E-5	0.0	4.143503446524732E-6	0.02292600456962134	0.0
10-11	1.9267291026340004E-4	5.3865544804821516E-5	0.0	4.143503446524732E-6	0.02875384216715838	0.0
12-13	2.1960568266581078E-4	6.422430342113334E-5	0.0	4.143503446524732E-6	0.03435585882685981	0.0
14-15	2.5896896540779574E-4	7.251131031418281E-5	0.0	4.143503446524732E-6	0.041822452037497376	0.0
16-17	2.8797348953346884E-4	7.458306203744517E-5	0.0	4.143503446524732E-6	0.047246298048998256	0.0
18-19	3.273367722754538E-4	7.665481376070753E-5	0.0	4.143503446524732E-6	0.054592729659686604	0.0
20-21	3.6462830329417643E-4	8.908532410028173E-5	0.0	4.143503446524732E-6	0.06023203785040676	4.143503446524732E-6
22-23	4.2056559982226024E-4	1.0980284133290539E-4	4.143503446524732E-6	4.143503446524732E-6	0.065966646620397	4.143503446524732E-6
24-25	4.847899032433936E-4	1.1394634477943013E-4	4.143503446524732E-6	4.143503446524732E-6	0.07073996259079349	4.143503446524732E-6
26-27	5.137944273690667E-4	1.2016159994921722E-4	4.143503446524732E-6	4.143503446524732E-6	0.07426608402378604	4.143503446524732E-6
28-29	6.17382013532185E-4	1.2016159994921722E-4	4.143503446524732E-6	4.143503446524732E-6	0.0781485467531797	4.143503446524732E-6
30-31	8.079831720723227E-4	1.3259211028879143E-4	8.287006893049464E-6	4.143503446524732E-6	0.08274783557882215	4.143503446524732E-6
32-33	9.675080547635248E-4	1.4502262062836562E-4	8.287006893049464E-6	4.143503446524732E-6	0.087160666749371	6.2152551697870985E-6
34-35	0.0011001001650523161	1.5330962752141509E-4	8.287006893049464E-6	4.143503446524732E-6	0.09018956776878057	8.287006893049464E-6
36-37	0.001139463447794301	1.574531309679398E-4	8.287006893049464E-6	4.143503446524732E-6	0.09329512360195086	8.287006893049464E-6
38-39	0.0011871137374293355	1.574531309679398E-4	8.287006893049464E-6	4.143503446524732E-6	0.09635924440065591	8.287006893049464E-6
40-41	0.0012181900132782711	1.6159663441446455E-4	8.287006893049464E-6	6.2152551697870985E-6	0.09914160696499726	8.287006893049464E-6
42-43	0.0012430510339574196	1.760988964773011E-4	8.287006893049464E-6	8.287006893049464E-6	0.10189496500521295	8.287006893049464E-6
44-45	0.0012554815442969937	2.071751723262366E-4	8.287006893049464E-6	8.287006893049464E-6	0.10410138059048736	1.2430510339574195E-5
46-47	0.001309347089101815	2.237491861123355E-4	8.287006893049464E-6	8.287006893049464E-6	0.10648182332051581	1.2430510339574195E-5
48-49	0.0013507821235670624	2.2996444128212261E-4	8.287006893049464E-6	8.287006893049464E-6	0.1088394767815884	1.2430510339574195E-5
50-51	0.0013942889097555721	2.444667033449592E-4	8.287006893049464E-6	8.287006893049464E-6	0.11087186522210878	1.2430510339574195E-5
52-53	0.0014481544545603938	2.5275371023800863E-4	8.287006893049464E-6	8.287006893049464E-6	0.1129891954832829	1.8645765509361292E-5
54-55	0.0015082352545350024	2.672559723008452E-4	8.287006893049464E-6	8.287006893049464E-6	0.11496150312382869	2.071751723262366E-5
56-57	0.0015662443027863486	2.817582343636818E-4	8.287006893049464E-6	8.287006893049464E-6	0.1170581158677702	2.071751723262366E-5
58-59	0.0016035358338050711	2.9418874470325595E-4	8.287006893049464E-6	8.287006893049464E-6	0.11897448621178788	2.071751723262366E-5
60-61	0.0016781188958425162	3.1904976538240433E-4	8.287006893049464E-6	8.287006893049464E-6	0.12045371694219723	2.071751723262366E-5
62-63	0.0017195539303077637	3.3355202744524094E-4	8.287006893049464E-6	8.287006893049464E-6	0.12246745961720823	2.486102067914839E-5
64-65	0.0017444149509869122	3.376955308917656E-4	8.287006893049464E-6	1.2430510339574197E-5	0.12439004521639571	2.6932772402410755E-5
66-67	0.0018169262613010949	3.5841304812438927E-4	8.287006893049464E-6	1.6574013786098928E-5	0.12567038778137185	2.9004524125673122E-5
68-69	0.0018459307854267679	3.770588136337506E-4	8.287006893049464E-6	1.6574013786098928E-5	0.12696937611185735	3.107627584893549E-5
70-71	0.0018604330474896046	3.812023170802753E-4	8.287006893049464E-6	1.6574013786098928E-5	0.1282994407181918	3.5219779295460224E-5
72-73	0.0018894375716152776	3.9363282741984953E-4	8.287006893049464E-6	1.6574013786098928E-5	0.12928352278674143	3.7291531018722584E-5
74-75	0.0019163703440176884	3.998480825896366E-4	8.287006893049464E-6	1.6574013786098928E-5	0.1304022687173031	3.7291531018722584E-5
76-77	0.0019453748681433616	4.081350894826861E-4	1.2430510339574195E-5	1.6574013786098928E-5	0.13170332879951185	3.7291531018722584E-5
78-79	0.001972307640545772	4.3713961360835924E-4	1.2430510339574195E-5	1.8645765509361292E-5	0.13329236237125408	3.7291531018722584E-5
80-81	0.001982666399162084	4.495701239479334E-4	1.8645765509361292E-5	2.2789268955886027E-5	0.13471979930858186	3.7291531018722584E-5
82-83	0.0019950969095016585	4.6614413773403234E-4	2.9004524125673122E-5	2.486102067914839E-5	0.13633990915617306	3.7291531018722584E-5
84-85	0.0020199579301808067	4.6821588945729467E-4	2.9004524125673122E-5	2.9004524125673122E-5	0.13799938228650618	3.7291531018722584E-5
86-87	0.0020468907025832173	4.7235939290381945E-4	2.9004524125673122E-5	3.3148027572197856E-5	0.13982045205125382	3.7291531018722584E-5
88-89	0.0020655364680925788	4.7650289635034417E-4	2.9004524125673122E-5	3.3148027572197856E-5	0.1416311630573851	3.7291531018722584E-5
90-91	0.0020738234749856283	4.827181515201312E-4	2.9004524125673122E-5	3.5219779295460224E-5	0.1426836129328024	3.7291531018722584E-5
92-93	0.0020800387301554155	4.889334066899184E-4	3.107627584893549E-5	3.7291531018722584E-5	0.1436241882151635	3.7291531018722584E-5
94-95	0.0020862539853252022	4.93076910136443E-4	3.3148027572197856E-5	3.7291531018722584E-5	0.14466213582851795	4.143503446524732E-5
96-97	0.002096612743941514	4.992921653062302E-4	3.5219779295460224E-5	3.7291531018722584E-5	0.14560063935915582	4.557853791177205E-5
98-99	0.0021048997508345637	5.179379308155915E-4	4.557853791177205E-5	3.936328274198495E-5	0.1465764344208124	4.557853791177205E-5
100-101	0.002111115006004351	5.220814342621162E-4	4.557853791177205E-5	4.143503446524732E-5	0.14750457919283394	4.557853791177205E-5
102-103	0.002117330261174138	5.303684411551657E-4	4.557853791177205E-5	4.143503446524732E-5	0.14838921717866696	4.972204135829678E-5
104-105	0.002121473764620663	5.345119446016904E-4	4.557853791177205E-5	4.143503446524732E-5	0.1494333800471912	5.3865544804821516E-5
106-107	0.0021339042749602367	5.427989514947399E-4	4.557853791177205E-5	4.143503446524732E-5	0.15023514796409373	5.3865544804821516E-5
108-109	0.0021339042749602367	5.697317238971505E-4	4.557853791177205E-5	4.143503446524732E-5	0.1509312565431099	5.8009048251346244E-5
110-111	0.0021380477784067615	5.904492411297743E-4	4.7650289635034414E-5	4.143503446524732E-5	0.1517889617565405	5.8009048251346244E-5
112-113	0.0021608370473626477	6.090950066391356E-4	5.3865544804821516E-5	4.143503446524732E-5	0.15252857712174517	6.215255169787098E-5
114-115	0.0021649805508091725	6.215255169787098E-4	5.593729652808388E-5	4.143503446524732E-5	0.15341735861102473	6.629605514439571E-5
116-117	0.002167052302532435	6.256690204252345E-4	5.8009048251346244E-5	4.143503446524732E-5	0.1542833508313484	6.629605514439571E-5
118-119	0.002169124054255697	6.298125238717592E-4	6.215255169787098E-5	4.143503446524732E-5	0.15506854473446482	7.043955859092043E-5
120-121	0.002179482812872009	6.380995307648087E-4	6.215255169787098E-5	4.143503446524732E-5	0.1558848149134302	7.043955859092043E-5
122-123	0.0021836263163185336	6.380995307648087E-4	6.215255169787098E-5	4.143503446524732E-5	0.1566389325406977	7.458306203744517E-5
124-125	0.002191913323211583	6.380995307648087E-4	6.215255169787098E-5	4.143503446524732E-5	0.15753392928514703	7.458306203744517E-5
126-127	0.0021981285783813703	6.401712824880711E-4	6.422430342113334E-5	4.350678618850968E-5	0.1585221548571432	7.87265654839699E-5
128-129	0.002214702592167469	6.505300411043829E-4	6.629605514439571E-5	4.557853791177205E-5	0.1594627301395043	8.079831720723228E-5
130-131	0.002231276605953568	6.608887997206947E-4	6.629605514439571E-5	4.7650289635034414E-5	0.16046960147700984	8.701357237701937E-5
132-133	0.0022437071162931424	6.671040548904818E-4	6.629605514439571E-5	4.972204135829678E-5	0.1613128044283776	8.908532410028173E-5
134-135	0.002266496385249028	6.712475583370065E-4	6.629605514439571E-5	5.179379308155915E-5	0.1621891554073176	9.530057927006883E-5
136-137	0.00227685514386534	6.712475583370065E-4	6.629605514439571E-5	5.3865544804821516E-5	0.16319809849654635	9.530057927006883E-5
138-139	0.0022789268955886025	6.774628135067936E-4	6.629605514439571E-5	5.3865544804821516E-5	0.16401436867551172	1.0151583443985594E-4
>>END_MODULE
