##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.2d.R.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22936848
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	59-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.903394136805545	37.0	35.0	38.0	33.0	39.0
2	35.2681450825327	35.0	34.0	37.0	33.0	39.0
3	35.4024824596649	35.0	34.0	37.0	33.0	39.0
4	35.456773964757495	36.0	35.0	38.0	33.0	39.0
5	35.36910769082134	36.0	35.0	37.0	33.0	39.0
6	35.326892474502166	35.0	34.0	37.0	33.0	39.0
7	35.36144312418167	35.0	34.0	37.0	33.0	39.0
8	35.43362802072892	36.0	34.0	38.0	33.0	39.0
9	35.438739751861284	36.0	34.0	38.0	33.0	39.0
10-14	35.42300261134398	35.8	34.4	37.8	33.0	39.0
15-19	35.39539103193255	36.0	34.0	38.0	33.0	39.0
20-24	35.37936287496869	36.0	34.0	38.0	33.0	39.0
25-29	35.35626125263593	36.0	34.0	38.0	33.0	39.0
30-34	35.337469681971996	36.0	34.0	38.0	32.8	39.0
35-39	35.30644094602711	36.0	34.0	37.8	32.0	39.0
40-44	35.2884496509721	35.8	34.0	37.8	32.0	39.0
45-49	35.254273751999406	36.0	34.0	37.2	32.0	39.0
50-54	35.23054998664158	35.8	34.0	37.2	32.0	39.0
55-59	35.19975243328987	35.8	34.0	37.2	32.0	39.0
60-64	35.155982790485545	35.0	34.0	37.0	32.0	39.0
65-69	35.124533828883266	35.0	34.0	37.0	32.0	39.0
70-74	35.090561887472504	35.0	34.0	37.0	32.0	39.0
75-79	35.03949036914132	35.0	34.0	37.0	32.0	39.0
80-84	34.99460574272665	35.0	34.0	37.0	32.0	39.0
85-89	34.942252519298286	35.0	34.0	37.0	31.2	39.0
90-94	34.88158693888242	35.0	34.0	37.0	31.0	39.0
95-99	34.812807494301104	35.0	34.0	37.0	31.0	39.0
100-104	34.75914655018692	35.0	34.0	37.0	31.0	39.0
105-109	34.692919540869596	35.0	34.0	37.0	30.8	39.0
110-114	34.613714080396946	35.0	34.0	37.0	30.0	39.0
115-119	34.54838605529075	35.0	34.0	37.0	30.0	39.0
120-124	34.468050116589026	35.0	34.0	37.0	30.0	39.0
125-129	34.38610305421834	35.0	34.0	37.0	30.0	39.0
130-134	34.27993048376361	35.0	34.0	37.0	29.4	39.0
135-139	34.21538295077902	35.0	34.0	37.0	29.0	39.0
140-144	34.08625489040296	35.0	34.0	37.0	29.0	39.0
145-149	34.07883414879912	35.0	34.0	37.0	29.0	39.0
150	34.912886491757085	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	154.0
22	1356.0
23	7852.0
24	28258.0
25	72244.0
26	147121.0
27	258897.0
28	382466.0
29	505930.0
30	650117.0
31	846845.0
32	1129809.0
33	1573929.0
34	2356623.0
35	3995173.0
36	9558001.0
37	1422062.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.660911037122446	18.606671675201405	16.261227349110914	31.47118993856523
2	24.311531383911163	25.39794046679823	33.05420169327538	17.23632645601523
3	19.90382026335964	29.2402731186081	29.877134818175538	20.97877179985672
4	23.540931632053145	33.81494708622742	22.29482738362665	20.349293898092785
5	25.164800353527276	35.061933542382725	22.45349883357972	17.319767270510276
6	18.924922367174034	38.68215286008722	22.94769047738008	19.445234295358667
7	20.337129799629224	20.57150906755828	37.95985399407202	21.131507138740467
8	21.542892435008817	23.168396564065855	28.433677566911676	26.855033434013652
9	23.440343677561973	23.139360735180354	28.819949454258058	24.60034613299962
10-14	23.15129960315384	28.977607559678642	25.689287385956433	22.18180545121108
15-19	22.96383705380966	28.15883769208393	27.13747154796509	21.739853706141314
20-24	23.048894076465956	28.258009121392792	26.995622938252023	21.697473863889233
25-29	23.145032831014966	28.213208719872927	26.941914599599738	21.699843849512366
30-34	23.12893210087105	27.994221350727877	27.124481968926155	21.752364579474914
35-39	23.210192612341505	27.993682479824606	27.08819973869121	21.707925169142683
40-44	23.364065542048323	27.76562673301929	27.180473969221925	21.68983375571046
45-49	23.3594668282233	27.606629297975033	27.266750863065404	21.767153010736262
50-54	23.436788699127273	27.602463076007655	27.214346103701782	21.746402121163293
55-59	23.609458457413155	27.48676278449419	27.121457141800825	21.782321616291828
60-64	23.51832839099463	27.460376746638282	27.192170746048923	21.829124116318166
65-69	23.598381252423067	27.400335938114868	27.145334853309983	21.855947956152082
70-74	23.694005740063407	27.345408189499746	27.059556878935766	21.90102919150108
75-79	23.618768076318304	27.403668466425408	27.135662210272077	21.84190124698421
80-84	23.71467819119811	27.448776985180963	26.990458537398464	21.846086286222462
85-89	23.749385128335394	27.395584127122202	27.03778837406952	21.81724237047288
90-94	23.68227075346311	27.3313292307505	27.109589969101183	21.876810046685204
95-99	23.758223706584104	27.432597503622098	27.109831691527734	21.69934709826606
100-104	23.9225205224729	27.396658633547773	26.93165567546543	21.7491651685139
105-109	23.704369165636937	27.527366970206508	27.03685991490718	21.73140394924938
110-114	23.724186499457872	27.51354945669989	26.98042938290929	21.781834660932947
115-119	23.807074743156743	27.494786148455034	27.011333471027548	21.68680563736067
120-124	23.776147171786246	27.413407531974787	27.180489095924788	21.62995620031418
125-129	23.753807941945883	27.507529193574754	27.132905797155928	21.605757067323438
130-134	23.81580512539419	27.41800585354848	27.187583289784715	21.578605731272617
135-139	23.731295447920765	27.425640095907877	27.279858528970095	21.563205927201263
140-144	23.75862646161069	27.54684368876741	27.25095610679546	21.443573742826445
145-149	23.47195160612386	27.633914005229077	27.225848477940286	21.668285910706782
150	23.58363838942593	26.43698219894978	27.66614764245889	22.313231769165405
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	0.5
4	0.0
5	0.0
6	0.0
7	0.0
8	1.5
9	4.0
10	4.0
11	4.0
12	7.5
13	15.0
14	27.0
15	44.0
16	68.5
17	111.0
18	172.5
19	298.0
20	513.5
21	833.5
22	1362.0
23	2323.5
24	3798.5
25	6168.5
26	9745.0
27	15560.0
28	25690.0
29	39012.5
30	52666.0
31	69213.0
32	92379.5
33	123440.0
34	165779.0
35	229046.0
36	321075.0
37	434194.0
38	569403.5
39	743255.5
40	947259.0
41	1142376.5
42	1319150.5
43	1481461.5
44	1602177.0
45	1661662.0
46	1666935.5
47	1622258.0
48	1527550.5
49	1389084.0
50	1212262.0
51	1020097.5
52	833415.0
53	664697.5
54	520400.5
55	394229.0
56	291815.5
57	215587.0
58	157487.0
59	112917.5
60	78231.5
61	52769.0
62	35915.0
63	24688.5
64	16637.5
65	11168.5
66	7909.5
67	5747.5
68	4812.0
69	3664.0
70	2175.0
71	1467.5
72	846.5
73	637.5
74	553.5
75	367.5
76	247.5
77	93.0
78	24.0
79	2.5
80	1.0
81	0.0
82	0.0
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	1.3079390856145534E-5
5	1.7439187808194046E-5
6	1.3079390856145535E-4
7	0.0022627346181131776
8	0.11356399100695962
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	8.720118855917616E-7
105-109	4.5345518418263904E-5
110-114	0.018773474200665576
115-119	0.0
120-124	0.0
125-129	8.721359784880334E-7
130-134	0.04727262755777186
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
58-59	1.0
60-61	0.0
62-63	0.0
64-65	2.0
66-67	0.0
68-69	7.0
70-71	21.0
72-73	13.0
74-75	10.0
76-77	29.0
78-79	42.0
80-81	49.0
82-83	66.0
84-85	66.0
86-87	86.0
88-89	109.0
90-91	134.0
92-93	122.0
94-95	135.0
96-97	132.0
98-99	171.0
100-101	189.0
102-103	170.0
104-105	183.0
106-107	196.0
108-109	219.0
110-111	208.0
112-113	222.0
114-115	224.0
116-117	271.0
118-119	275.0
120-121	310.0
122-123	343.0
124-125	394.0
126-127	472.0
128-129	493.0
130-131	630.0
132-133	806.0
134-135	1084.0
136-137	1669.0
138-139	3464.0
140-141	7102.0
142-143	11234.0
144-145	69705.0
146-147	797432.0
148-149	1071449.0
150-151	2.0966909E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.37975733904558
#Duplication Level	Percentage of total
1	30.075005038558444
2	10.635515084108938
3	7.123724634271432
4	5.050246959488808
5	3.781940598326234
6	3.03983319201535
7	2.4981955158641487
8	2.0835815251570895
9	1.8488821455822604
>10	20.96456040960586
>50	5.202210766258263
>100	5.971891628391329
>500	0.8995880401485823
>1k	0.728412826086061
>5k	0.09641163613710456
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.359796952048511E-6	2.615878171229107E-5	4.359796952048511E-6	0.0	6.408901519511312E-4	0.0
2	2.179898476024256E-5	4.359796952048512E-5	4.359796952048511E-6	4.359796952048511E-6	0.004508030048418161	0.0
3	5.231756342458214E-5	4.359796952048512E-5	4.359796952048511E-6	4.359796952048511E-6	0.00612115492067611	0.0
4	1.2643411160940683E-4	4.359796952048512E-5	4.359796952048511E-6	4.359796952048511E-6	0.007494490960571392	0.0
5	1.2643411160940683E-4	4.795776647253363E-5	4.359796952048511E-6	4.359796952048511E-6	0.008968102330363789	0.0
6	1.4387329941760088E-4	5.231756342458214E-5	4.359796952048511E-6	4.359796952048511E-6	0.010664063344710659	0.0
7	1.8311147198603748E-4	6.103715732867916E-5	4.359796952048511E-6	4.359796952048511E-6	0.012665210145700927	0.0
8	2.964661927392988E-4	6.539695428072768E-5	4.359796952048511E-6	4.359796952048511E-6	0.01775745298569359	0.0
9	3.0082598969134733E-4	7.41165481848247E-5	4.359796952048511E-6	8.719593904097023E-6	0.02384808932770536	0.0
10-11	3.2916466987966265E-4	8.501604056494597E-5	4.359796952048511E-6	8.719593904097023E-6	0.029746894603826995	0.0
12-13	3.66222943972075E-4	8.93758375169945E-5	4.359796952048511E-6	8.719593904097023E-6	0.035556324042431635	0.0
14-15	4.098209134925601E-4	9.3735634469043E-5	4.359796952048511E-6	1.089949238012128E-5	0.043626308200673425	0.0
16-17	4.468791875849725E-4	9.591553294506726E-5	4.359796952048511E-6	1.3079390856145534E-5	0.049124012157206604	0.0
18-19	5.144560403417244E-4	9.809543142109151E-5	4.359796952048511E-6	1.3079390856145534E-5	0.0564484710366481	0.0
20-21	5.733132991943793E-4	1.0681502532518853E-4	4.359796952048511E-6	1.3079390856145534E-5	0.06221212260725624	0.0
22-23	6.234509641429371E-4	1.286140100854311E-4	4.359796952048511E-6	1.3079390856145534E-5	0.06821338311175101	0.0
24-25	6.844881214716163E-4	1.329738070374796E-4	4.359796952048511E-6	1.9619086284218302E-5	0.07325112848984307	0.0
26-27	7.564247711804167E-4	1.3515370551350387E-4	4.359796952048511E-6	2.179898476024256E-5	0.07703325234574515	0.0
28-29	9.242769538342845E-4	1.4605319789362514E-4	4.359796952048511E-6	2.179898476024256E-5	0.08088949274983206	0.0
30-31	0.0012120235526694861	1.5477279179772216E-4	8.719593904097023E-6	2.179898476024256E-5	0.08580080401631471	0.0
32-33	0.0014278335017958875	1.722119796059162E-4	8.719593904097023E-6	2.179898476024256E-5	0.09072955447060554	0.0
34-35	0.0015956856844497552	1.78751675033989E-4	8.719593904097023E-6	2.179898476024256E-5	0.0937443540629471	6.539695428072767E-6
36-37	0.001661082638730483	1.87471268938086E-4	8.719593904097023E-6	2.179898476024256E-5	0.0968725083760419	8.719593904097023E-6
38-39	0.0017177599991071136	1.87471268938086E-4	8.719593904097023E-6	2.179898476024256E-5	0.10019903345045492	8.719593904097023E-6
40-41	0.0017591780701515744	1.87471268938086E-4	8.719593904097023E-6	2.179898476024256E-5	0.10291954674853318	8.719593904097023E-6
42-43	0.00178751675033989	1.9837076131820727E-4	8.719593904097023E-6	2.179898476024256E-5	0.10550272644262193	8.719593904097023E-6
44-45	0.001798416242720011	2.2452954303049834E-4	8.719593904097023E-6	2.179898476024256E-5	0.10769352441102631	8.719593904097023E-6
46-47	0.001839834313764472	2.441486293147166E-4	8.719593904097023E-6	2.179898476024256E-5	0.11017860867369396	1.3079390856145534E-5
48-49	0.001909591064997248	2.5286822321881366E-4	8.719593904097023E-6	2.179898476024256E-5	0.11244134329180713	1.3079390856145534E-5
50-51	0.0019488292375656846	2.5286822321881366E-4	8.719593904097023E-6	2.179898476024256E-5	0.11444249009279742	1.3079390856145534E-5
52-53	0.0020164060903224367	2.5940791864688644E-4	8.719593904097023E-6	2.179898476024256E-5	0.11649159466026021	1.9619086284218302E-5
54-55	0.0020665437552709947	2.768471064550805E-4	8.719593904097023E-6	2.179898476024256E-5	0.11819191547155913	2.179898476024256E-5
56-57	0.0021406603034558195	2.8338680188315325E-4	8.719593904097023E-6	2.179898476024256E-5	0.12003392968379963	2.179898476024256E-5
58-59	0.0022016974607844985	2.921063957872503E-4	1.089949238012128E-5	2.179898476024256E-5	0.12179310775395119	2.179898476024256E-5
60-61	0.002258374821161129	3.0736568511942005E-4	1.3079390856145534E-5	2.179898476024256E-5	0.12319478247403479	2.3978883236266812E-5
62-63	0.002291073298301493	3.1826517749954135E-4	1.3079390856145534E-5	2.179898476024256E-5	0.12504987607713144	2.615878171229107E-5
64-65	0.0023063325876336626	3.4224406073580816E-4	1.3079390856145534E-5	2.179898476024256E-5	0.12681559384271107	2.615878171229107E-5
66-67	0.0023695496434383663	3.705827409241235E-4	1.3079390856145534E-5	2.179898476024256E-5	0.12809737414661335	2.615878171229107E-5
68-69	0.002393528526674633	3.74942537876172E-4	1.3079390856145534E-5	2.179898476024256E-5	0.12928977861299862	2.615878171229107E-5
70-71	0.0024044280190547544	3.74942537876172E-4	1.3079390856145534E-5	2.179898476024256E-5	0.13049526247024001	2.615878171229107E-5
72-73	0.002441486293147167	3.9238172568436603E-4	1.3079390856145534E-5	2.179898476024256E-5	0.13137158165760177	3.051857866433958E-5
74-75	0.002454565684003312	3.989214211124388E-4	1.3079390856145534E-5	2.179898476024256E-5	0.13237869475352498	3.051857866433958E-5
76-77	0.002465465176383433	4.2072040587268135E-4	1.7439187808194046E-5	2.179898476024256E-5	0.13355366003210206	3.051857866433958E-5
78-79	0.002498163653523797	4.3815959368087543E-4	2.179898476024256E-5	2.179898476024256E-5	0.1349771337369459	3.051857866433958E-5
80-81	0.002515602841331991	4.59958578441118E-4	2.179898476024256E-5	2.3978883236266812E-5	0.13624365475151598	3.051857866433958E-5
82-83	0.0025265023337121125	4.77397766249312E-4	2.3978883236266812E-5	2.615878171229107E-5	0.13784152033444177	3.051857866433958E-5
84-85	0.002548301318472355	4.8611736015340904E-4	3.051857866433958E-5	2.615878171229107E-5	0.13936744926765876	3.051857866433958E-5
86-87	0.002574460100184646	4.970168525335303E-4	3.051857866433958E-5	2.615878171229107E-5	0.14109828865762203	3.051857866433958E-5
88-89	0.0026006188818969373	4.970168525335303E-4	3.051857866433958E-5	2.615878171229107E-5	0.14289234510338997	3.487837561638809E-5
90-91	0.002624597765133204	5.166359388177487E-4	3.051857866433958E-5	2.615878171229107E-5	0.14382970144808038	3.9238172568436604E-5
92-93	0.002639857054465374	5.188158372937729E-4	3.051857866433958E-5	2.615878171229107E-5	0.14473871911258251	3.9238172568436604E-5
94-95	0.0026463967498934465	5.253555327218457E-4	3.051857866433958E-5	2.615878171229107E-5	0.14562811769080042	3.9238172568436604E-5
96-97	0.0026551163437975437	5.36255025101967E-4	3.051857866433958E-5	2.615878171229107E-5	0.14644775951778555	5.0137664948557884E-5
98-99	0.0026616560392256163	5.44974619006064E-4	3.051857866433958E-5	2.615878171229107E-5	0.14726740134477068	5.667736037663065E-5
100-101	0.0026660158361776647	5.44974619006064E-4	3.051857866433958E-5	2.615878171229107E-5	0.1480456251007113	5.667736037663065E-5
102-103	0.0026703756331297136	5.44974619006064E-4	3.051857866433958E-5	2.615878171229107E-5	0.14880422977036775	5.667736037663065E-5
104-105	0.0026790952270338104	5.536942129101609E-4	3.051857866433958E-5	2.615878171229107E-5	0.14962387159735285	5.667736037663065E-5
106-107	0.002700894211794053	5.580540098622095E-4	3.051857866433958E-5	2.615878171229107E-5	0.15040209535329352	5.667736037663065E-5
108-109	0.0027074339072221255	5.733132991943793E-4	3.487837561638809E-5	2.615878171229107E-5	0.15123045677418273	5.667736037663065E-5
110-111	0.0027117937041741744	5.820328930984764E-4	3.487837561638809E-5	2.615878171229107E-5	0.15215691362649306	6.103715732867916E-5
112-113	0.0027161535011262228	5.951122839546218E-4	3.487837561638809E-5	2.615878171229107E-5	0.15293077758548168	6.103715732867916E-5
114-115	0.0027248730950303196	6.038318778587189E-4	3.487837561638809E-5	2.615878171229107E-5	0.1538681339301721	6.103715732867916E-5
116-117	0.0027314127904583927	6.060117763347431E-4	3.9238172568436604E-5	2.615878171229107E-5	0.15483600885352686	6.103715732867916E-5
118-119	0.0027401323843624895	6.147313702388401E-4	4.795776647253363E-5	2.615878171229107E-5	0.15560987281251548	6.539695428072768E-5
120-121	0.0027488519782665867	6.147313702388401E-4	4.795776647253363E-5	2.615878171229107E-5	0.15638373677150408	6.975675123277618E-5
122-123	0.002753211775218635	6.147313702388401E-4	4.795776647253363E-5	3.051857866433958E-5	0.1570834841823079	6.975675123277618E-5
124-125	0.0027597514706467078	6.147313702388401E-4	4.795776647253363E-5	3.051857866433958E-5	0.15789222651691287	6.975675123277618E-5
126-127	0.0027706509630268292	6.234509641429371E-4	4.795776647253363E-5	3.051857866433958E-5	0.15879906428293897	7.41165481848247E-5
128-129	0.0027815504554069507	6.256308626189614E-4	4.795776647253363E-5	3.051857866433958E-5	0.15973206083067734	7.847634513687321E-5
130-131	0.0028011695416911685	6.408901519511312E-4	5.2317563424582144E-5	3.051857866433958E-5	0.16068467646469994	7.847634513687321E-5
132-133	0.002838227815783581	6.496097458552283E-4	6.103715732867916E-5	3.051857866433958E-5	0.16138224397702772	7.847634513687321E-5
134-135	0.0028643865974958722	6.583293397593253E-4	6.103715732867916E-5	3.051857866433958E-5	0.16220406570248885	8.065624361289745E-5
136-137	0.002873106191399969	6.714087306154708E-4	6.321705580470342E-5	3.051857866433958E-5	0.16296921006757337	9.591553294506725E-5
138-139	0.0028774659883520175	6.801283245195678E-4	6.539695428072768E-5	3.269847714036383E-5	0.16376705290979826	1.0027532989711577E-4
>>END_MODULE
