##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.2d.S.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24133957
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	63-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.985272079501925	37.0	35.0	38.0	33.0	39.0
2	35.34511381618854	35.0	34.0	37.0	33.0	39.0
3	35.459910283257734	35.0	34.0	37.0	33.0	39.0
4	35.54334769884607	36.0	35.0	38.0	33.0	39.0
5	35.467828835528294	36.0	35.0	38.0	33.0	39.0
6	35.41370447457083	35.0	35.0	37.0	33.0	39.0
7	35.448028104135595	35.0	34.0	38.0	33.0	39.0
8	35.577940119807124	36.0	35.0	38.0	33.0	39.0
9	35.57587672009194	36.0	35.0	38.0	33.0	39.0
10-14	35.52587226371539	35.8	34.8	37.8	33.0	39.0
15-19	35.50182994856583	36.0	35.0	38.0	33.0	39.0
20-24	35.47161307198815	36.0	34.8	38.0	33.0	39.0
25-29	35.47028811727807	36.0	34.2	38.0	33.0	39.0
30-34	35.45543616407372	36.0	34.0	38.0	33.0	39.0
35-39	35.433638884829364	36.0	34.0	38.0	33.0	39.0
40-44	35.41547597022735	36.0	34.0	38.0	33.0	39.0
45-49	35.39161745419535	36.0	34.0	38.0	33.0	39.0
50-54	35.37347819091582	36.0	34.0	38.0	32.4	39.0
55-59	35.347226374854316	36.0	34.0	38.0	32.0	39.0
60-64	35.30954775711325	36.0	34.0	38.0	32.0	39.0
65-69	35.27725345152697	36.0	34.0	38.0	32.0	39.0
70-74	35.26208482619289	36.0	34.0	38.0	32.0	39.0
75-79	35.21014793451893	36.0	34.0	38.0	32.0	39.0
80-84	35.136804370259284	36.0	34.0	38.0	32.0	39.0
85-89	35.12766545181569	36.0	34.0	38.0	32.0	39.0
90-94	35.08618499925046	36.0	34.0	38.0	32.0	39.0
95-99	35.02257060500527	35.2	34.0	38.0	32.0	39.0
100-104	34.963099904507246	35.2	34.0	38.0	31.0	39.0
105-109	34.91466509677616	35.0	34.0	37.6	31.0	39.0
110-114	34.846552557921505	35.0	34.0	37.2	31.0	39.0
115-119	34.789110364259535	35.0	34.0	37.0	31.0	39.0
120-124	34.72252461815058	35.0	34.0	37.0	30.6	39.0
125-129	34.64737907794962	35.0	34.0	37.0	30.0	39.0
130-134	34.57574641866337	35.0	34.0	37.0	30.0	39.0
135-139	34.497776545082516	35.0	34.0	37.0	30.0	39.0
140-144	34.41197946069405	35.0	34.0	37.0	30.0	39.0
145-149	34.37507644262504	35.0	34.0	37.0	29.8	39.0
150	35.141387044641355	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	9.0
21	154.0
22	1486.0
23	8058.0
24	27660.0
25	69406.0
26	138937.0
27	244608.0
28	363879.0
29	483233.0
30	621870.0
31	809415.0
32	1082145.0
33	1508249.0
34	2256868.0
35	3837921.0
36	1.03846E7
37	2295452.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.20212263575343	18.757118859538867	15.692785066286477	32.34797343842122
2	24.19677800867881	25.548118777206742	32.74601425700725	17.5090889571072
3	19.94307439927899	29.295129679728856	29.849307347319797	20.91248857367236
4	23.4399108277188	34.225833749517335	21.539070447502663	20.795184975261204
5	25.38370728016131	35.01562135044825	21.643421341970566	17.95725002741987
6	19.15441049117683	38.83626041250759	21.948125703055066	20.061203393260516
7	20.375548029691114	20.5475173424731	36.965914872559026	22.111019755276768
8	21.59255939670399	23.321310301497594	28.04753899246609	27.038591309332322
9	23.552569518541862	23.58640980424387	27.45171875461616	25.409301922598104
10-14	23.12356485925619	28.808378998934987	25.153241965252526	22.914814176556295
15-19	23.18156529407921	27.64888990230653	26.69071880752916	22.4788259960851
20-24	23.085890669078445	27.611225556473883	26.646037763377468	22.656846011070204
25-29	23.167943822888223	27.22053826481915	26.637045885181614	22.974472027111013
30-34	22.988981234937818	26.947212402402243	26.85346979654824	23.210336566111696
35-39	23.29192100574307	26.68299939375876	26.873959375994577	23.15112022450359
40-44	23.452000846773696	26.330372594929212	26.96226565747175	23.255360900825337
45-49	23.37006815749278	26.164310311815008	27.053315790692757	23.412305739999454
50-54	23.566364970862093	26.188201998974375	26.77231261404212	23.473120416121414
55-59	23.631330742820168	26.19250543953484	26.798716845314672	23.377446972330315
60-64	23.334138892632808	26.19126508090874	26.850034802415824	23.62456122404263
65-69	23.550122881638185	26.16110721609184	26.791084342593223	23.497685559676757
70-74	23.715879253466028	26.1324854147807	26.513219558244604	23.638415773508665
75-79	23.560484943384186	26.249934645518998	26.764491637771403	23.425088773325417
80-84	23.858688481931765	26.233663407716644	26.4575148884242	23.450133221927388
85-89	23.6115051979813	26.30233656297969	26.656206338037293	23.429951901001722
90-94	23.645511360594607	26.10963922257988	26.645489979767774	23.599359437057746
95-99	23.64559474363262	26.32087946096322	26.691638874634478	23.34188692076968
100-104	23.884053201888435	26.0789732800013	26.647821816001642	23.38915170210862
105-109	23.65889285686257	26.301634266830675	26.564237603071668	23.475235273235086
110-114	23.77116300737252	26.381971851025064	26.50581201900853	23.341053122593884
115-119	23.874201230096947	26.434546242008572	26.701945722514964	22.989306805379513
120-124	23.834973610457734	26.552749087421113	26.82786058662579	22.784416715495365
125-129	23.811033971793474	26.617060150782606	26.673034543223253	22.89887133420067
130-134	23.99063600597447	26.61385126408209	26.644830740937035	22.750681989006406
135-139	23.75195564843122	26.642163838613786	26.910648282063526	22.695232230891467
140-144	23.77806530520718	26.805696294186227	26.704957471216524	22.711280929390078
145-149	23.554893217411763	26.981562649266937	26.605956750153048	22.85758738316825
150	23.802217566886206	25.86046756503002	26.830381399815188	23.50693346826858
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	12.0
3	28.0
4	38.5
5	39.0
6	25.5
7	15.0
8	15.5
9	13.0
10	7.5
11	6.5
12	9.0
13	12.5
14	16.0
15	28.5
16	51.5
17	94.0
18	158.0
19	228.0
20	386.0
21	618.5
22	985.0
23	1622.5
24	2597.0
25	4230.0
26	6541.5
27	10103.5
28	15363.0
29	22127.5
30	31829.0
31	46784.5
32	68186.5
33	93921.5
34	127032.5
35	181035.0
36	259955.5
37	352705.5
38	466370.0
39	609884.0
40	781267.0
41	966985.0
42	1158283.0
43	1351822.5
44	1502884.0
45	1595826.5
46	1629189.0
47	1603184.5
48	1555296.0
49	1504645.5
50	1400640.5
51	1241665.5
52	1062668.0
53	892258.5
54	751712.5
55	637663.5
56	529965.5
57	428715.5
58	354690.0
59	290875.5
60	220898.5
61	149031.5
62	91681.5
63	55541.0
64	34340.0
65	17206.5
66	9295.0
67	5101.5
68	2708.0
69	1608.5
70	942.5
71	740.0
72	505.0
73	384.0
74	313.5
75	200.5
76	132.0
77	46.0
78	9.5
79	3.5
80	3.0
81	0.5
82	0.5
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	4.143539329252969E-6
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.03288727165628082
25-29	0.0
30-34	8.287078658505939E-7
35-39	0.0
40-44	0.0
45-49	0.0
50-54	3.3148314634023757E-6
55-59	0.0
60-64	0.0
65-69	1.6574158003768633E-6
70-74	8.287079894668231E-7
75-79	7.085457009159482E-4
80-84	0.06424902311096092
85-89	3.3148460776499544E-6
90-94	8.287142115313412E-7
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	8.288884878910582E-7
140-144	3.7818590838003494E-4
145-149	0.03354707665093474
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62-63	1.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	3.0
72-73	2.0
74-75	6.0
76-77	7.0
78-79	13.0
80-81	17.0
82-83	19.0
84-85	22.0
86-87	32.0
88-89	24.0
90-91	41.0
92-93	39.0
94-95	46.0
96-97	56.0
98-99	64.0
100-101	78.0
102-103	72.0
104-105	91.0
106-107	84.0
108-109	94.0
110-111	104.0
112-113	107.0
114-115	130.0
116-117	150.0
118-119	144.0
120-121	177.0
122-123	218.0
124-125	236.0
126-127	258.0
128-129	331.0
130-131	443.0
132-133	581.0
134-135	784.0
136-137	1361.0
138-139	4445.0
140-141	8340.0
142-143	9254.0
144-145	61420.0
146-147	790424.0
148-149	1031234.0
150-151	2.2223005E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.23246250457214
#Duplication Level	Percentage of total
1	24.73340840410885
2	8.354955031049458
3	5.222806460603004
4	3.763095474683532
5	2.862684022161393
6	2.233798885597996
7	1.8162534565724662
8	1.5492440695911058
9	1.417848098674269
>10	17.358225964440976
>50	6.03034698253723
>100	13.004137837985496
>500	4.260838111808662
>1k	5.895868389089993
>5k	0.9173169809758392
>10k+	0.5791718301196662
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	28909	0.11978557846937408	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.480573036572494E-4	4.143539329252969E-6	0.0	0.0	0.0023038078670646507	0.0
2	0.0018438750015175713	1.2430617987758907E-5	4.143539329252969E-6	0.0	0.009198657310941591	0.0
3	0.0026228603954171295	1.2430617987758907E-5	4.143539329252969E-6	0.0	0.012832541302696445	0.0
4	0.0063396151737570424	1.2430617987758907E-5	4.143539329252969E-6	0.0	0.018231573048713066	0.0
5	0.006550935679548944	1.2430617987758907E-5	4.143539329252969E-6	0.0	0.022072634006930568	0.0
6	0.007628255905154716	1.6574157317011876E-5	4.143539329252969E-6	0.0	0.028031043562396336	0.0
7	0.00993620731154862	2.9004775304770785E-5	4.143539329252969E-6	0.0	0.037072246378826314	0.0
8	0.017527171362740058	2.9004775304770785E-5	4.143539329252969E-6	0.0	0.05007052925469288	0.0
9	0.018202568273408295	2.9004775304770785E-5	4.143539329252969E-6	0.0	0.06018076521807012	0.0
10-11	0.018919400577369058	2.9004775304770785E-5	4.143539329252969E-6	0.0	0.09997531693621564	0.0
12-13	0.019739821364561143	2.9004775304770785E-5	4.143539329252969E-6	0.0	0.11803286133310009	0.0
14-15	0.02273767206927567	2.9004775304770785E-5	4.143539329252969E-6	0.0	0.12711757131248722	0.0
16-17	0.026299044122768594	2.9004775304770785E-5	4.143539329252969E-6	0.0	0.13343646878959797	0.0
18-19	0.033452864774723846	3.522008429865023E-5	4.143539329252969E-6	0.0	0.14011999772768302	0.0
20-21	0.03971375270122508	3.9363623627903206E-5	4.143539329252969E-6	0.0	0.1447027522258368	0.0
22-23	0.04216672798414284	5.1794241615662115E-5	4.143539329252969E-6	0.0	0.14930208088130761	0.0
24-25	0.047851663943877916	6.008132027416805E-5	4.143539329252969E-6	0.0	0.15368594549165726	0.0
26-27	0.05272446619507941	7.458370792655344E-5	4.143539329252969E-6	0.0	0.1570712171236569	0.0
28-29	0.06406533333924477	7.458370792655344E-5	4.143539329252969E-6	2.0717696646264845E-6	0.16017887162059663	0.0
30-31	0.08802327774098545	7.458370792655344E-5	8.287078658505938E-6	4.143539329252969E-6	0.16362215280320586	0.0
32-33	0.10752691736377917	8.287078658505939E-5	8.287078658505938E-6	4.143539329252969E-6	0.16646876432240265	0.0
34-35	0.1214533530493984	8.701432591431236E-5	1.0358848323132422E-5	4.143539329252969E-6	0.1692242179763559	0.0
36-37	0.12382138577606648	9.322963490819181E-5	1.2430617987758907E-5	4.143539329252969E-6	0.17091892556202035	0.0
38-39	0.12599260038459503	9.53014045728183E-5	1.2430617987758907E-5	4.143539329252969E-6	0.17239816910256367	0.0
40-41	0.12740140375654105	9.737317423744478E-5	1.2430617987758907E-5	4.143539329252969E-6	0.17386291025545458	0.0
42-43	0.12768109266126562	9.944494390207126E-5	1.2430617987758907E-5	4.143539329252969E-6	0.17523856531276658	0.0
44-45	0.1284538627461713	9.944494390207126E-5	1.2430617987758907E-5	4.143539329252969E-6	0.17651270365651184	0.0
46-47	0.12981294364616627	1.0358848323132423E-4	1.4502387652385392E-5	4.143539329252969E-6	0.17780755969690343	0.0
48-49	0.13109744083823469	1.0358848323132423E-4	1.6574157317011876E-5	4.143539329252969E-6	0.1788330856808935	0.0
50-51	0.13214989982786496	1.0358848323132423E-4	1.6574157317011876E-5	4.143539329252969E-6	0.18007821924933404	2.0717696646264845E-6
52-53	0.1331567798848734	1.0358848323132423E-4	1.6574157317011876E-5	4.143539329252969E-6	0.1812715585761589	4.143539329252969E-6
54-55	0.13368508114935318	1.0773202256057719E-4	1.6574157317011876E-5	6.2153089938794535E-6	0.18217692191960067	4.143539329252969E-6
56-57	0.13456351148715479	1.1601910121908314E-4	1.6574157317011876E-5	1.2430617987758907E-5	0.183173443128286	4.143539329252969E-6
58-59	0.13474582721764194	1.222344102129626E-4	1.6574157317011876E-5	1.2430617987758907E-5	0.18416167725831284	8.287078658505938E-6
60-61	0.13501930081337263	1.243061798775891E-4	1.6574157317011876E-5	1.2430617987758907E-5	0.1849240884948954	8.287078658505938E-6
62-63	0.13523476485849378	1.2844971920684205E-4	1.6574157317011876E-5	1.2430617987758907E-5	0.18578594467538	8.287078658505938E-6
64-65	0.13525755432480469	1.388085675299745E-4	1.6574157317011876E-5	1.2430617987758907E-5	0.18668923624915718	8.287078658505938E-6
66-67	0.13536321457770062	1.4088033719460096E-4	1.6574157317011876E-5	1.2430617987758907E-5	0.18739363793513014	8.287078658505938E-6
68-69	0.1354398700552918	1.4295210685922742E-4	1.6574157317011876E-5	1.2430617987758907E-5	0.18805867599747528	8.287078658505938E-6
70-71	0.13552895615087074	1.5745449451161282E-4	1.6574157317011876E-5	1.2430617987758907E-5	0.1886884939755217	8.287078658505938E-6
72-73	0.135582822162151	1.6574157317011877E-4	1.6574157317011876E-5	1.2430617987758907E-5	0.18917950338603817	1.6574157317011876E-5
74-75	0.13561389870712043	1.7402865182862472E-4	1.6574157317011876E-5	1.2430617987758907E-5	0.18974509650448124	1.6574157317011876E-5
76-77	0.13562425755544355	1.7817219115787768E-4	2.0717696646264847E-5	1.2430617987758907E-5	0.19038734510051541	1.6574157317011876E-5
78-79	0.13566154940940683	1.7817219115787768E-4	2.0717696646264847E-5	1.2430617987758907E-5	0.19133207206758512	1.6574157317011876E-5
80-81	0.13571127188135787	1.8231573048713064E-4	2.4861235975517814E-5	1.2430617987758907E-5	0.19214834931544794	2.0717696646264847E-5
82-83	0.13572991780833948	1.864592698163836E-4	2.6933005640144298E-5	1.4502387652385392E-5	0.19290661701270123	2.0717696646264847E-5
84-85	0.13577342497129666	1.9060280914563656E-4	2.9004775304770785E-5	1.6574157317011876E-5	0.1936711000189484	2.0717696646264847E-5
86-87	0.1358086450555953	2.0303342713339547E-4	2.9004775304770785E-5	2.0717696646264847E-5	0.19435478400827516	2.278946631089133E-5
88-89	0.13585422398821712	2.0924873612727495E-4	2.9004775304770785E-5	2.0717696646264847E-5	0.19512962586284543	2.4861235975517814E-5
90-91	0.13587908522419262	2.154640451211544E-4	2.9004775304770785E-5	2.0717696646264847E-5	0.19571593667793474	2.4861235975517814E-5
92-93	0.13588944407251574	2.154640451211544E-4	3.522008429865023E-5	2.0717696646264847E-5	0.1962670274087254	2.9004775304770785E-5
94-95	0.13593709477480215	2.154640451211544E-4	3.729185396327672E-5	2.4861235975517814E-5	0.19691756308341812	3.314831463402375E-5
96-97	0.13595366893211916	2.1753581478578087E-4	3.9363623627903206E-5	2.4861235975517814E-5	0.1975349504434768	4.143539329252969E-5
98-99	0.1359660995501069	2.278946631089133E-4	4.143539329252969E-5	2.4861235975517814E-5	0.19801352923600551	5.593778094491509E-5
100-101	0.1360013196344056	2.3203820243816628E-4	4.143539329252969E-5	2.4861235975517814E-5	0.19849210802853423	6.215308993879454E-5
102-103	0.13601582202205798	2.3203820243816628E-4	4.143539329252969E-5	2.4861235975517814E-5	0.19906805999530042	6.215308993879454E-5
104-105	0.1360427550276981	2.3203820243816628E-4	4.143539329252969E-5	2.4861235975517814E-5	0.19960879187776792	6.215308993879454E-5
106-107	0.13606761626367364	2.3203820243816628E-4	4.143539329252969E-5	2.4861235975517814E-5	0.2000210740410286	6.215308993879454E-5
108-109	0.136098692808643	2.3618174176741924E-4	4.143539329252969E-5	2.4861235975517814E-5	0.20052865760886207	6.422485960342102E-5
110-111	0.1361359846626063	2.486123597551782E-4	5.38660112802886E-5	2.4861235975517814E-5	0.20104867179468333	6.8368398932674E-5
112-113	0.1361587741289172	2.527558990844311E-4	5.38660112802886E-5	2.4861235975517814E-5	0.20157697305916306	7.458370792655344E-5
114-115	0.13618570713455735	2.568994384136841E-4	5.38660112802886E-5	2.4861235975517814E-5	0.20205969539102103	7.458370792655344E-5
116-117	0.13619192244355122	2.610429777429371E-4	5.38660112802886E-5	2.4861235975517814E-5	0.2025486330318729	7.665547759117992E-5
118-119	0.1361981377525451	2.69330056401443E-4	5.38660112802886E-5	2.4861235975517814E-5	0.2031183696896452	7.872724725580641E-5
120-121	0.13620849660086823	2.69330056401443E-4	5.38660112802886E-5	2.4861235975517814E-5	0.20371918289238686	7.872724725580641E-5
122-123	0.13622092721885598	2.69330056401443E-4	5.800955060954157E-5	2.4861235975517814E-5	0.20425784300518973	7.872724725580641E-5
124-125	0.13623750137617302	2.73473595730696E-4	5.800955060954157E-5	2.4861235975517814E-5	0.20484001028094978	8.07990169204329E-5
126-127	0.1362520037638254	2.73473595730696E-4	5.800955060954157E-5	2.6933005640144298E-5	0.2053911010117404	8.287078658505939E-5
128-129	0.13626650615147778	2.73473595730696E-4	5.800955060954157E-5	3.314831463402375E-5	0.20596290943917733	9.115786524356532E-5
130-131	0.1362768649998009	2.755453653953225E-4	6.0081320274168056E-5	3.314831463402375E-5	0.20663001927118707	9.115786524356532E-5
132-133	0.13628308030879477	2.817606743892019E-4	6.215308993879454E-5	3.314831463402375E-5	0.20726812432789204	9.322963490819181E-5
134-135	0.13628929561778866	2.817606743892019E-4	6.215308993879454E-5	3.314831463402375E-5	0.20782543036767653	1.0151671356669774E-4
136-137	0.13629343915711792	2.859042137184549E-4	6.215308993879454E-5	3.314831463402375E-5	0.20836616225014407	1.0980379222520368E-4
138-139	0.13629343915711792	2.8797598338308134E-4	6.215308993879454E-5	3.314831463402375E-5	0.20891103767194083	1.1601910121908314E-4
>>END_MODULE
