##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.2d.S.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24108909
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	67-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.05146097652117	37.0	35.0	38.0	33.0	39.0
2	35.357770274880544	35.0	34.0	37.0	33.0	39.0
3	35.51623381215633	35.0	34.0	37.0	33.0	39.0
4	35.56177378246357	36.0	35.0	38.0	33.0	39.0
5	35.47681543781181	36.0	35.0	37.0	33.0	39.0
6	35.42878120283253	35.0	35.0	37.0	33.0	39.0
7	35.47463636782569	35.0	34.0	38.0	33.0	39.0
8	35.56920941549035	36.0	35.0	38.0	33.0	39.0
9	35.55108802310382	36.0	35.0	38.0	33.0	39.0
10-14	35.533186972500495	35.8	34.8	37.8	33.0	39.0
15-19	35.51081892589997	36.0	35.0	38.0	33.0	39.0
20-24	35.50087013891836	36.0	35.0	38.0	33.0	39.0
25-29	35.48560336761817	36.0	34.6	38.0	33.0	39.0
30-34	35.47188995570061	36.0	34.4	38.0	33.0	39.0
35-39	35.443311955758766	36.0	34.0	38.0	33.0	39.0
40-44	35.43051045569918	36.0	34.0	38.0	33.0	39.0
45-49	35.40179138757378	36.0	34.0	38.0	33.0	39.0
50-54	35.38186442198608	36.0	34.0	38.0	32.6	39.0
55-59	35.35891026839912	36.0	34.0	38.0	32.2	39.0
60-64	35.319728810623495	36.0	34.0	38.0	32.0	39.0
65-69	35.2954698515722	36.0	34.0	38.0	32.0	39.0
70-74	35.26541730375753	36.0	34.0	38.0	32.0	39.0
75-79	35.21928617411551	36.0	34.0	38.0	32.0	39.0
80-84	35.179509765045765	36.0	34.0	38.0	32.0	39.0
85-89	35.13422630089215	36.0	34.0	38.0	32.0	39.0
90-94	35.084606219812855	36.0	34.0	38.0	32.0	39.0
95-99	35.021066684210595	35.2	34.0	38.0	32.0	39.0
100-104	34.976009947307666	35.2	34.0	38.0	31.0	39.0
105-109	34.922115997287634	35.0	34.0	37.6	31.0	39.0
110-114	34.84826350408183	35.0	34.0	37.0	31.0	39.0
115-119	34.77786917983218	35.0	34.0	37.0	31.0	39.0
120-124	34.707591836383	35.0	34.0	37.0	30.6	39.0
125-129	34.63243336718009	35.0	34.0	37.0	30.0	39.0
130-134	34.53037440227396	35.0	34.0	37.0	30.0	39.0
135-139	34.4779544221438	35.0	34.0	37.0	30.0	39.0
140-144	34.361214578703866	35.0	34.0	37.0	30.0	39.0
145-149	34.34650389650714	35.0	34.0	37.0	29.6	39.0
150	35.105662872697586	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	155.0
22	1417.0
23	8113.0
24	28795.0
25	72032.0
26	141707.0
27	238470.0
28	351495.0
29	468237.0
30	609337.0
31	803300.0
32	1084219.0
33	1524296.0
34	2298245.0
35	3900401.0
36	1.0279236E7
37	2299446.0
38	3.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.95614874152953	18.995430278491657	14.348115047429147	32.70030593254967
2	23.85017090570129	26.502269347816608	32.57089733923671	17.076662407245387
3	19.67084864769285	29.69628779137206	28.987238700847062	21.645624860088027
4	23.178037629160244	34.445337198792366	21.50577614275287	20.870849029294526
5	25.15071503235588	34.987696871724886	21.87612056605299	17.98546752986624
6	19.093974762607466	38.709196670824056	22.32718618664992	19.869642379918563
7	20.383124761058248	20.728030455463582	36.710134830240555	22.178709953237618
8	21.5478311357847	23.515502090949035	28.158308615292384	26.778358157973887
9	23.441666315136864	23.54029375613803	27.855565757869837	25.162474170855265
10-14	23.164516486415874	28.667897829802254	25.207699776045445	22.959885907736428
15-19	23.177434532603694	27.696237934283964	26.669706206946152	22.45662132616619
20-24	23.11081185797333	27.60201965173953	26.619303262540832	22.667865227746304
25-29	23.149841413396185	27.249251303740042	26.6001120166823	23.000795266181477
30-34	23.017126988201746	26.929214424427084	26.80508188902285	23.248576698348316
35-39	23.307054029753516	26.695019062828756	26.828437793086678	23.16948911433105
40-44	23.50571815589001	26.28676809887996	26.88563800211781	23.321875743112226
45-49	23.39028530905318	26.11654471797127	26.994780228338	23.498389744637553
50-54	23.62044421006359	26.1277455566322	26.71116722867883	23.540643004625387
55-59	23.656643276558057	26.139463216688902	26.714580074942422	23.489313431810622
60-64	23.361281093225745	26.12434598347026	26.796949625551285	23.71742329775271
65-69	23.566119016538458	26.029435045092587	26.766085574957728	23.63836036341123
70-74	23.75224521858936	26.017807747494142	26.458527983097763	23.771419050818736
75-79	23.598422264353676	26.112363143997346	26.706806343441812	23.582408248207162
80-84	23.900602856532316	26.12428882869579	26.392155565686824	23.582952749085067
85-89	23.66301429733138	26.219874971166142	26.58446539129427	23.532645340208212
90-94	23.670579425009073	25.996472181195575	26.601114786627505	23.73183360716785
95-99	23.718954482329185	26.160082423723356	26.639490740915488	23.48147235303197
100-104	23.95121673045187	25.967006836717182	26.587434242041848	23.4943421907891
105-109	23.717980667943756	26.157630045788267	26.502046674138764	23.62234261212922
110-114	23.822741010430654	26.23406193025435	26.428447948040017	23.514749111274977
115-119	23.968986470854972	26.33226746062809	26.56686017103707	23.13188589747987
120-124	23.9219463730112	26.492652926204613	26.69200326054874	22.893397440235447
125-129	23.90784851181625	26.52991491254795	26.529568117326562	23.03266845830923
130-134	24.075201656765934	26.542458200868424	26.511376323319798	22.87096381904584
135-139	23.87180538661433	26.558193145781033	26.775174927029884	22.794826540574753
140-144	23.909630763575947	26.684417559909534	26.585433167801426	22.820518508713093
145-149	23.655124669433587	26.717624532023486	26.613807609289225	23.013443189253703
150	23.96283672592783	25.554506057328275	26.839871963215696	23.6427852535282
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	0.5
7	1.0
8	1.5
9	2.5
10	4.0
11	4.5
12	5.5
13	12.5
14	20.5
15	32.0
16	53.5
17	94.5
18	175.5
19	292.0
20	438.0
21	647.5
22	1004.5
23	1597.5
24	2594.5
25	4106.0
26	6361.5
27	9840.5
28	14799.5
29	21174.5
30	30251.5
31	44132.5
32	64303.5
33	88716.0
34	120565.5
35	173512.0
36	250061.0
37	340302.5
38	454281.0
39	598121.0
40	768098.0
41	954218.0
42	1147608.5
43	1341152.5
44	1490795.0
45	1589116.0
46	1625463.5
47	1597581.0
48	1555026.0
49	1503730.0
50	1398421.5
51	1244322.5
52	1072468.0
53	906423.0
54	762385.5
55	650268.5
56	544683.5
57	440110.5
58	364933.5
59	302523.0
60	232088.0
61	157682.5
62	97312.5
63	58965.5
64	36244.5
65	17529.0
66	9263.5
67	5029.0
68	2614.5
69	1659.5
70	1046.5
71	872.5
72	584.0
73	425.0
74	358.5
75	223.0
76	148.5
77	59.0
78	15.5
79	2.0
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	8.295688535719307E-7
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	8.295688742174656E-7
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	2.4889608570998885E-6
130-134	0.05627365785645892
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
66-67	1.0
68-69	4.0
70-71	4.0
72-73	6.0
74-75	9.0
76-77	8.0
78-79	11.0
80-81	10.0
82-83	16.0
84-85	21.0
86-87	26.0
88-89	28.0
90-91	45.0
92-93	42.0
94-95	55.0
96-97	55.0
98-99	75.0
100-101	77.0
102-103	82.0
104-105	93.0
106-107	110.0
108-109	108.0
110-111	110.0
112-113	141.0
114-115	127.0
116-117	139.0
118-119	183.0
120-121	195.0
122-123	256.0
124-125	271.0
126-127	320.0
128-129	350.0
130-131	476.0
132-133	621.0
134-135	844.0
136-137	1343.0
138-139	4524.0
140-141	8478.0
142-143	9244.0
144-145	60445.0
146-147	786872.0
148-149	1004472.0
150-151	2.2228612E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.27429421867972
#Duplication Level	Percentage of total
1	24.955587657057553
2	8.192389178449517
3	5.1534035275172565
4	3.6756877706336075
5	2.803839903011917
6	2.197621335781346
7	1.7799323886722802
8	1.5413757274174251
9	1.3451373020581727
>10	16.91965667728265
>50	5.904220295461719
>100	13.165572402389975
>500	4.393918929973187
>1k	6.210862574737179
>5k	0.9607907823324024
>10k+	0.8000035472239504
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	28400	0.11779877720721414	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.2353185289305295E-4	4.147844267859653E-6	0.0	0.0	0.0017006161498224577	0.0
2	0.0014683368708223172	1.2443532803578959E-5	0.0	0.0	0.008876386733219657	0.0
3	0.0022149488390370547	1.2443532803578959E-5	0.0	0.0	0.012771212500739872	0.0
4	0.005421232458092566	1.2443532803578959E-5	0.0	4.147844267859653E-6	0.018694334115243457	0.0
5	0.005657659581360567	1.2443532803578959E-5	4.147844267859653E-6	4.147844267859653E-6	0.022779960719085213	0.0
6	0.006731951246736217	1.2443532803578959E-5	4.147844267859653E-6	4.147844267859653E-6	0.029988914056625293	0.0
7	0.009029856971130465	1.2443532803578959E-5	4.147844267859653E-6	4.147844267859653E-6	0.041175650047042774	0.0
8	0.017354580416724788	1.2443532803578959E-5	4.147844267859653E-6	4.147844267859653E-6	0.05436164697456861	0.0
9	0.018146818671885983	1.6591377071438612E-5	4.147844267859653E-6	4.147844267859653E-6	0.06437039519291396	0.0
10-11	0.018945278693448964	1.6591377071438612E-5	4.147844267859653E-6	4.147844267859653E-6	0.10404037777072367	0.0
12-13	0.019951130928404934	1.6591377071438612E-5	4.147844267859653E-6	4.147844267859653E-6	0.12350413699765511	0.0
14-15	0.02389573082713946	2.0739221339298265E-5	4.147844267859653E-6	4.147844267859653E-6	0.13338222812156286	0.0
16-17	0.028145197279561676	2.0739221339298265E-5	4.147844267859653E-6	4.147844267859653E-6	0.13996693089679005	0.0
18-19	0.03566109109292337	2.4887065607157918E-5	4.147844267859653E-6	4.147844267859653E-6	0.14640438520050825	0.0
20-21	0.04176879177734671	2.4887065607157918E-5	4.147844267859653E-6	4.147844267859653E-6	0.15103545332557355	0.0
22-23	0.04451259076053586	3.3182754142877224E-5	4.147844267859653E-6	4.147844267859653E-6	0.15569970420478174	0.0
24-25	0.05117195473258454	3.3182754142877224E-5	4.147844267859653E-6	8.295688535719306E-6	0.160183523858338	0.0
26-27	0.057246472662865006	3.3182754142877224E-5	4.147844267859653E-6	8.295688535719306E-6	0.16376933522790268	0.0
28-29	0.07142587829254322	3.525667627680705E-5	4.147844267859653E-6	8.295688535719306E-6	0.16731366815478876	0.0
30-31	0.09845945330831851	3.733059841073688E-5	2.0739221339298265E-5	8.295688535719306E-6	0.1712790072748626	0.0
32-33	0.1203331100548764	3.733059841073688E-5	2.0739221339298265E-5	8.295688535719306E-6	0.17407880215566784	0.0
34-35	0.13586263899374296	3.94045205446667E-5	2.0739221339298265E-5	8.295688535719306E-6	0.17706939787279466	0.0
36-37	0.13852762893584275	4.147844267859653E-5	2.0739221339298265E-5	8.295688535719306E-6	0.17894007563759934	4.147844267859653E-6
38-39	0.1409561917546746	4.562628694645618E-5	2.0739221339298265E-5	8.295688535719306E-6	0.18038559936494844	4.147844267859653E-6
40-41	0.1426070337732827	4.9774131214315836E-5	2.0739221339298265E-5	8.295688535719306E-6	0.18189541467844936	4.147844267859653E-6
42-43	0.1429720440688544	4.9774131214315836E-5	2.0739221339298265E-5	8.295688535719306E-6	0.18336789939353954	4.147844267859653E-6
44-45	0.14383064783230132	5.5995897616105313E-5	2.0739221339298265E-5	8.295688535719306E-6	0.184595661296826	8.295688535719306E-6
46-47	0.14504804012491812	6.221766401789479E-5	2.0739221339298265E-5	8.295688535719306E-6	0.18587112340919285	8.295688535719306E-6
48-49	0.1461804016100438	7.05133525536141E-5	2.0739221339298265E-5	8.295688535719306E-6	0.1870138545049882	8.295688535719306E-6
50-51	0.14733765016077666	7.466119682147376E-5	2.0739221339298265E-5	8.295688535719306E-6	0.18812340284664064	8.295688535719306E-6
52-53	0.14851978577711666	7.88090410893334E-5	2.0739221339298265E-5	8.295688535719306E-6	0.18919354666774843	8.295688535719306E-6
54-55	0.14903411846633127	8.50308074911229E-5	2.4887065607157918E-5	8.295688535719306E-6	0.19008118534107038	8.295688535719306E-6
56-57	0.1497869522009478	8.917865175898254E-5	2.4887065607157918E-5	1.2443532803578959E-5	0.19110777679736565	8.295688535719306E-6
58-59	0.14992383106178717	9.33264960268422E-5	3.11088320089474E-5	1.2443532803578959E-5	0.1919186803517322	8.295688535719306E-6
60-61	0.15018307132852837	1.0162218456256149E-4	3.733059841073688E-5	1.2443532803578959E-5	0.19262381387726835	1.2443532803578959E-5
62-63	0.15051489886995717	1.0369610669649133E-4	3.733059841073688E-5	1.2443532803578959E-5	0.1934264217430992	1.2443532803578959E-5
64-65	0.1505460077019661	1.1199179523221063E-4	3.733059841073688E-5	1.2443532803578959E-5	0.19419999469905502	1.2443532803578959E-5
66-67	0.15062066889878759	1.2443532803578958E-4	3.733059841073688E-5	1.2443532803578959E-5	0.1948035060400286	1.4517454937508785E-5
68-69	0.15071399539481445	1.327310165715089E-4	3.733059841073688E-5	1.2443532803578959E-5	0.19548790034422547	1.6591377071438612E-5
70-71	0.15083428287858236	1.327310165715089E-4	3.733059841073688E-5	1.2443532803578959E-5	0.1960540810867883	1.6591377071438612E-5
72-73	0.15087576132126096	1.327310165715089E-4	3.733059841073688E-5	1.2443532803578959E-5	0.19657048769813681	1.6591377071438612E-5
74-75	0.15090064838686812	1.327310165715089E-4	3.733059841073688E-5	1.2443532803578959E-5	0.19722792101459258	1.6591377071438612E-5
76-77	0.15094005290741278	1.3895278297329836E-4	3.733059841073688E-5	1.2443532803578959E-5	0.19786876295397687	1.6591377071438612E-5
78-79	0.15100227057143067	1.6176592644652648E-4	3.733059841073688E-5	1.2443532803578959E-5	0.19862781845499522	1.6591377071438612E-5
80-81	0.15106448823544857	1.721355371161756E-4	4.147844267859653E-5	1.2443532803578959E-5	0.19938272611174568	1.6591377071438612E-5
82-83	0.1510873013789218	1.804312256518949E-4	4.147844267859653E-5	1.2443532803578959E-5	0.2001770382890408	1.6591377071438612E-5
84-85	0.15112463197733253	1.8250514778582474E-4	4.147844267859653E-5	1.2443532803578959E-5	0.20086350651537155	1.6591377071438612E-5
86-87	0.15116818434214505	1.8250514778582474E-4	4.147844267859653E-5	1.2443532803578959E-5	0.20151471806542554	1.6591377071438612E-5
88-89	0.15120551494055579	1.866529920536844E-4	4.147844267859653E-5	1.2443532803578959E-5	0.20226133003364027	1.6591377071438612E-5
90-91	0.1512138106290915	1.866529920536844E-4	4.147844267859653E-5	1.2443532803578959E-5	0.20274040604657806	1.6591377071438612E-5
92-93	0.15122625416189509	1.866529920536844E-4	4.147844267859653E-5	1.2443532803578959E-5	0.20317592969470333	1.6591377071438612E-5
94-95	0.15125736299390402	1.949486805894037E-4	4.147844267859653E-5	1.2443532803578959E-5	0.2037711453471412	1.6591377071438612E-5
96-97	0.15126358476030582	2.0531829125905283E-4	4.147844267859653E-5	1.2443532803578959E-5	0.2043290304011683	1.6591377071438612E-5
98-99	0.15127810221524335	2.115400576608423E-4	4.562628694645618E-5	1.2443532803578959E-5	0.20493461566427582	1.6591377071438612E-5
100-101	0.15130091535871656	2.115400576608423E-4	4.9774131214315836E-5	1.6591377071438612E-5	0.2054696875748297	1.6591377071438612E-5
102-103	0.15130713712511834	2.1568790192870197E-4	4.9774131214315836E-5	1.6591377071438612E-5	0.20601305517391932	1.866529920536844E-5
104-105	0.15131958065792195	2.177618240626318E-4	4.9774131214315836E-5	1.6591377071438612E-5	0.2064983529532589	2.0739221339298265E-5
106-107	0.15134031987926122	2.198357461965616E-4	5.1848053348245664E-5	1.866529920536844E-5	0.20698779857686633	2.0739221339298265E-5
108-109	0.15135691125633266	2.447228118037195E-4	5.392197548217549E-5	2.0739221339298265E-5	0.2074585789012684	2.0739221339298265E-5
110-111	0.15139216793260948	2.447228118037195E-4	5.806981975003514E-5	2.0739221339298265E-5	0.20798535512328659	2.0739221339298265E-5
112-113	0.15141083323181487	2.4679673393764934E-4	5.806981975003514E-5	2.0739221339298265E-5	0.2085432401773137	2.0739221339298265E-5
114-115	0.1514274246088863	2.50944578205509E-4	5.806981975003514E-5	2.0739221339298265E-5	0.2090990513092069	2.0739221339298265E-5
116-117	0.15143986814168986	2.592402667412283E-4	5.806981975003514E-5	2.0739221339298265E-5	0.2095511663344036	2.281314347322809E-5
118-119	0.1514419420638238	2.6131418887515815E-4	5.806981975003514E-5	2.4887065607157918E-5	0.21001365097026997	2.903490987501757E-5
120-121	0.15144401598595775	2.6131418887515815E-4	5.806981975003514E-5	2.4887065607157918E-5	0.2105321315037524	2.903490987501757E-5
122-123	0.15145438559662738	2.6131418887515815E-4	5.806981975003514E-5	2.4887065607157918E-5	0.21100498575028842	2.903490987501757E-5
124-125	0.15146682912943094	2.6131418887515815E-4	5.806981975003514E-5	2.6960987741087746E-5	0.211490283529628	2.903490987501757E-5
126-127	0.15148756835077024	2.6131418887515815E-4	5.806981975003514E-5	2.903490987501757E-5	0.211992172686039	2.903490987501757E-5
128-129	0.1514979379614399	2.6131418887515815E-4	5.806981975003514E-5	2.903490987501757E-5	0.2125625012728697	3.733059841073688E-5
130-131	0.15150830757210954	2.737577216787371E-4	6.014374188396497E-5	2.903490987501757E-5	0.21322615635572725	3.733059841073688E-5
132-133	0.15152282502704706	2.737577216787371E-4	6.221766401789479E-5	2.903490987501757E-5	0.21377367179908474	3.733059841073688E-5
134-135	0.15154149032625241	2.820534102144564E-4	6.221766401789479E-5	2.903490987501757E-5	0.21427141311122788	3.733059841073688E-5
136-137	0.15155808170332385	2.820534102144564E-4	6.221766401789479E-5	3.1108832008947396E-5	0.21476915442337105	4.147844267859653E-5
138-139	0.15155808170332385	3.007187094198248E-4	6.221766401789479E-5	3.3182754142877224E-5	0.21525652612484455	4.147844267859653E-5
>>END_MODULE
