##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.4d.R.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21817240
Total Bases	3.2 Gbp
Sequences flagged as poor quality	0
Sequence length	59-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.98794517546674	37.0	35.0	38.0	33.0	39.0
2	35.38703438198416	35.0	34.0	37.0	33.0	39.0
3	35.48393563988846	35.0	34.0	37.0	33.0	39.0
4	35.55663310299561	36.0	35.0	38.0	33.0	39.0
5	35.47549355463844	36.0	35.0	37.0	33.0	39.0
6	35.42616187932112	35.0	35.0	37.0	33.0	39.0
7	35.446225187053905	35.0	34.0	37.0	33.0	39.0
8	35.53278361515939	36.0	35.0	38.0	33.0	39.0
9	35.53931835557568	36.0	35.0	38.0	33.0	39.0
10-14	35.52232083434935	35.8	35.0	37.8	33.0	39.0
15-19	35.50091030762828	36.0	35.0	38.0	33.0	39.0
20-24	35.4821093227191	36.0	35.0	38.0	33.0	39.0
25-29	35.46522740731641	36.0	35.0	38.0	33.0	39.0
30-34	35.44278350515464	36.0	34.4	38.0	33.0	39.0
35-39	35.41771552222004	36.0	34.0	38.0	33.0	39.0
40-44	35.395784948050256	36.0	34.0	38.0	33.0	39.0
45-49	35.366928016559385	36.0	34.0	38.0	33.0	39.0
50-54	35.34160198081884	36.0	34.0	38.0	32.6	39.0
55-59	35.31493014698468	36.0	34.0	38.0	32.2	39.0
60-64	35.270277985220766	36.0	34.0	38.0	32.0	39.0
65-69	35.24244262571885	36.0	34.0	38.0	32.0	39.0
70-74	35.21018634726708	36.0	34.0	38.0	32.0	39.0
75-79	35.160409342769604	36.0	34.0	37.6	32.0	39.0
80-84	35.11744778793387	35.4	34.0	37.2	32.0	39.0
85-89	35.06626568182981	35.0	34.0	37.0	32.0	39.0
90-94	35.00859402022205	35.0	34.0	37.0	32.0	39.0
95-99	34.94241111933879	35.0	34.0	37.0	31.0	39.0
100-104	34.8918401649065	35.0	34.0	37.0	31.0	39.0
105-109	34.82845173485538	35.0	34.0	37.0	31.0	39.0
110-114	34.75311120968022	35.0	34.0	37.0	31.0	39.0
115-119	34.68952910215721	35.0	34.0	37.0	30.8	39.0
120-124	34.61288481144354	35.0	34.0	37.0	30.0	39.0
125-129	34.53302442597449	35.0	34.0	37.0	30.0	39.0
130-134	34.431689213661905	35.0	34.0	37.0	30.0	39.0
135-139	34.369560155771254	35.0	34.0	37.0	30.0	39.0
140-144	34.247610600507016	35.0	34.0	37.0	29.0	39.0
145-149	34.236232867639806	35.0	34.0	37.0	29.2	39.0
150	35.01218732424409	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	104.0
22	1196.0
23	6684.0
24	24132.0
25	61272.0
26	123475.0
27	214474.0
28	320693.0
29	431323.0
30	566144.0
31	748768.0
32	1014306.0
33	1428159.0
34	2159449.0
35	3724331.0
36	9538579.0
37	1454134.0
38	10.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.80854498552521	19.427219024954578	16.713246038453995	31.05098995106622
2	24.58748219298133	25.760934930357827	32.5805005582741	17.071082318386743
3	19.98376513252822	29.7803159336378	30.2290573876439	20.006861546190077
4	23.57679631231065	33.96944498797488	22.62981122405085	19.823947475663626
5	25.286266810907378	35.38741895512511	22.47245457421413	16.853859659753386
6	18.904741300679003	39.39778761913754	22.737576098146324	18.959894982037127
7	20.254595926151662	21.293911238085826	37.976286770629216	20.475206065133296
8	21.256570223168364	23.782786530376573	28.606382402211295	26.354260844243765
9	23.283155889562565	23.385153209113525	29.1210574756477	24.21063342567621
10-14	22.927492203413447	29.499185964860818	25.842445698906	21.730876132819734
15-19	22.882833025625608	28.363417187508595	27.281173053970164	21.472576732895636
20-24	22.94370873676047	28.457826012822885	27.15965080825989	21.438814442156755
25-29	23.02918609319969	28.340182351204827	27.07805386932536	21.552577686270126
30-34	23.021552680357367	28.169705242276294	27.24157959485251	21.56716248251383
35-39	23.14347919351852	28.12869913884616	27.177396407611592	21.550425260023726
40-44	23.277619900592374	27.946950210017402	27.269598721011455	21.50583116837877
45-49	23.298212789518747	27.77212883022784	27.343887677818092	21.585770702435322
50-54	23.36870566579457	27.718147666707615	27.30537959888602	21.607767068611793
55-59	23.511738423375274	27.5860997999747	27.241740935150364	21.660420841499658
60-64	23.42192153645106	27.57045655502055	27.287803924227077	21.71981798430131
65-69	23.51723838895083	27.49287856628585	27.26627240957562	21.7236106351877
70-74	23.615223725569567	27.4160013918544	27.1700700188969	21.798704863679134
75-79	23.534037367177326	27.482971248661915	27.24676484228047	21.736226541880292
80-84	23.652772418708953	27.52690531638196	27.081844727860823	21.73847753704827
85-89	23.67045051731954	27.50315909623355	27.149623766773573	21.67676661967334
90-94	23.62064691993739	27.41727570424859	27.199572541688894	21.76250483412513
95-99	23.6718783446561	27.53256867113258	27.202759853795182	21.592793130416137
100-104	23.870116116979897	27.47571548461331	27.010349517045274	21.64381888136152
105-109	23.632356140955075	27.617559081224975	27.13304157845327	21.617043199366684
110-114	23.678952827150532	27.600225493899227	27.052715347127652	21.668106331822585
115-119	23.735302743111454	27.60441712480049	27.093911135515086	21.56636899657297
120-124	23.679107909686746	27.52907102487874	27.242511703690496	21.549309361744022
125-129	23.670269935591595	27.61853035283789	27.206906060826856	21.504293650743662
130-134	23.734210648877713	27.517731631097238	27.282454116288722	21.46560360373633
135-139	23.60734954269141	27.530688775495932	27.39324525847514	21.468716423337515
140-144	23.646457598124325	27.65095166239532	27.346731926913865	21.355858812566485
145-149	23.391661055874618	27.8346401664743	27.221159261029353	21.552539516621728
150	23.481044525363178	26.634275318437755	27.649735047965386	22.234945108233685
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.5
4	0.5
5	0.5
6	0.5
7	0.5
8	3.0
9	7.5
10	6.5
11	6.0
12	10.5
13	14.5
14	25.0
15	46.5
16	82.5
17	145.5
18	253.5
19	438.5
20	673.5
21	1102.0
22	1755.0
23	2819.0
24	4682.0
25	7607.5
26	11697.0
27	18483.0
28	29865.5
29	44081.5
30	58786.5
31	76826.5
32	102365.5
33	134820.0
34	177563.5
35	242115.5
36	336651.5
37	449911.5
38	582507.5
39	754252.5
40	952828.5
41	1130572.0
42	1286035.0
43	1434901.5
44	1539643.5
45	1580297.0
46	1566894.5
47	1506935.5
48	1408113.5
49	1273309.0
50	1104749.5
51	924507.5
52	752684.5
53	597484.0
54	464840.5
55	350626.0
56	258312.0
57	191115.0
58	139922.5
59	100094.0
60	68967.5
61	45604.0
62	30967.5
63	21394.0
64	14226.0
65	9375.5
66	6508.5
67	4908.5
68	4369.5
69	3176.0
70	1635.5
71	1044.0
72	554.0
73	360.5
74	323.5
75	222.5
76	143.5
77	62.5
78	21.0
79	3.0
80	2.0
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	4.5835311890963294E-6
5	2.7501187134577975E-5
6	9.167062378192658E-5
7	0.002168010252442564
8	0.11188857985703049
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	1.8334993047462313E-6
105-109	5.317232703812097E-5
110-114	0.018973699432709674
115-119	0.0
120-124	0.0
125-129	9.168599804811512E-7
130-134	0.047230047567378856
135-139	0.0
140-144	0.0
145-149	9.35677811322347E-7
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
58-59	1.0
60-61	0.0
62-63	0.0
64-65	2.0
66-67	1.0
68-69	4.0
70-71	8.0
72-73	7.0
74-75	15.0
76-77	25.0
78-79	23.0
80-81	31.0
82-83	48.0
84-85	37.0
86-87	65.0
88-89	95.0
90-91	106.0
92-93	103.0
94-95	105.0
96-97	88.0
98-99	126.0
100-101	146.0
102-103	126.0
104-105	142.0
106-107	151.0
108-109	173.0
110-111	149.0
112-113	161.0
114-115	170.0
116-117	205.0
118-119	254.0
120-121	264.0
122-123	292.0
124-125	341.0
126-127	386.0
128-129	452.0
130-131	511.0
132-133	673.0
134-135	957.0
136-137	1411.0
138-139	3001.0
140-141	6526.0
142-143	9509.0
144-145	62186.0
146-147	753764.0
148-149	946624.0
150-151	2.0027776E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.40394432474396
#Duplication Level	Percentage of total
1	29.705608596322897
2	11.16885229539314
3	7.231869552912746
4	5.227804601875675
5	3.9755258543230565
6	3.0840129684924533
7	2.5052186865349566
8	2.1944387653380737
9	1.803099115684545
>10	20.769578105338073
>50	5.001349870156272
>100	5.621582294922916
>500	0.8080749078108104
>1k	0.8342984393321244
>5k	0.06868594556206786
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.3750593567288987E-5	4.5835311890963294E-6	0.0	0.0	7.196143966881237E-4	0.0
2	3.2084718323674305E-5	2.2917655945481648E-5	0.0	4.5835311890963294E-6	0.005211474962002526	0.0
3	5.041884308005962E-5	2.2917655945481648E-5	0.0	4.5835311890963294E-6	0.007040303906451962	0.0
4	1.1917181091650456E-4	2.2917655945481648E-5	0.0	4.5835311890963294E-6	0.00851620094934098	0.0
5	1.237553421056009E-4	2.2917655945481648E-5	0.0	4.5835311890963294E-6	0.010239608676441199	0.0
6	1.2833887329469722E-4	2.2917655945481648E-5	0.0	4.5835311890963294E-6	0.012132607057537983	0.0
7	1.3292240448379354E-4	3.2084718323674305E-5	0.0	4.5835311890963294E-6	0.014153944311929465	0.0
8	1.8334124756385318E-4	3.6668249512770636E-5	0.0	4.5835311890963294E-6	0.020020864233972768	0.0
9	1.9250830994204583E-4	4.5835311890963296E-5	0.0	4.5835311890963294E-6	0.02633697021254751	0.0
10-11	2.2230126267117198E-4	4.812707748551146E-5	0.0	4.5835311890963294E-6	0.03298996573352083	0.0
12-13	2.5438598099484627E-4	5.729413986370411E-5	0.0	4.5835311890963294E-6	0.03890272096745509	0.0
14-15	2.8188716812942426E-4	6.416943664734861E-5	0.0	4.5835311890963294E-6	0.04750600900938891	0.0
16-17	3.116801208585504E-4	6.416943664734861E-5	0.0	4.5835311890963294E-6	0.05337980422821585	0.0
18-19	3.6897426072225454E-4	6.875296783644493E-5	0.0	4.5835311890963294E-6	0.06184787810007132	0.0
20-21	4.010589790459288E-4	7.792003021463761E-5	0.0	4.5835311890963294E-6	0.068113565235566	0.0
22-23	4.377272285586995E-4	1.0083768616011924E-4	0.0	4.5835311890963294E-6	0.07416382640517316	0.0
24-25	4.789790092605664E-4	1.0771298294376374E-4	0.0	4.5835311890963294E-6	0.07950364024047038	0.0
26-27	5.133554931787889E-4	1.168800453219564E-4	0.0	9.167062378192659E-6	0.08331713818979852	0.0
28-29	6.119014137443599E-4	1.2146357651105272E-4	0.0	1.1458827972740822E-5	0.08756148807090172	0.0
30-31	7.585744117954425E-4	1.2833887329469722E-4	0.0	1.3750593567288987E-5	0.09231231814840007	0.0
32-33	8.800379883064952E-4	1.5125652924017887E-4	0.0	1.3750593567288987E-5	0.09711356706897847	0.0
34-35	9.854592056557107E-4	1.5813182602382336E-4	0.0	1.3750593567288987E-5	0.1002647447614822	0.0
36-37	0.0010244192207630295	1.627153572129197E-4	0.0	1.3750593567288987E-5	0.10377572965232999	0.0
38-39	0.00108629689181583	1.6500712280746786E-4	0.0	1.3750593567288987E-5	0.10715608390428855	0.0
40-41	0.001127548672517697	1.695906539965642E-4	0.0	1.3750593567288987E-5	0.11002079089747374	0.0
42-43	0.0011367157348958897	1.81049481969305E-4	0.0	1.8334124756385318E-5	0.11295195909290084	0.0
44-45	0.001182551046786853	1.9480007553659398E-4	0.0	1.8334124756385318E-5	0.11530560235850182	0.0
46-47	0.0012238028274887199	2.4063538742755727E-4	2.2917655945481647E-6	1.8334124756385318E-5	0.11792050690188127	0.0
48-49	0.0012467204834342017	2.589695121839426E-4	4.5835311890963294E-6	1.8334124756385318E-5	0.1203154019481841	4.5835311890963294E-6
50-51	0.0013017228577033575	2.635530433730389E-4	4.5835311890963294E-6	1.8334124756385318E-5	0.12248799573181576	4.5835311890963294E-6
52-53	0.0013544334663779653	2.727201057512316E-4	4.5835311890963294E-6	1.8334124756385318E-5	0.12467434010901471	4.5835311890963294E-6
54-55	0.0013888099502961878	2.8647069931852056E-4	4.5835311890963294E-6	1.8334124756385318E-5	0.12645962550716772	4.5835311890963294E-6
56-57	0.0014369370277816994	2.910542305076169E-4	4.5835311890963294E-6	2.2917655945481648E-5	0.12862763575961028	4.5835311890963294E-6
58-59	0.001501106464429048	2.979295272912614E-4	4.5835311890963294E-6	2.2917655945481648E-5	0.13061918006127266	4.5835311890963294E-6
60-61	0.0015904853226164262	3.048048240749059E-4	4.5835311890963294E-6	2.2917655945481648E-5	0.13210424416653985	6.875296783644494E-6
62-63	0.001629445337723745	3.1397188645309855E-4	4.5835311890963294E-6	2.2917655945481648E-5	0.1342424614662533	9.167062378192659E-6
64-65	0.0016729888840201603	3.2772248002038755E-4	4.5835311890963294E-6	2.2917655945481648E-5	0.13622942223672654	9.167062378192659E-6
66-67	0.0017646595078020869	3.391813079931284E-4	4.5835311890963294E-6	2.2917655945481648E-5	0.13761364865583364	9.167062378192659E-6
68-69	0.0018036195229094057	3.391813079931284E-4	4.5835311890963294E-6	2.7501187134577975E-5	0.13917204926012638	9.167062378192659E-6
70-71	0.0018196618820712426	3.506401359658692E-4	4.5835311890963294E-6	2.7501187134577975E-5	0.14069148984931185	1.3750593567288987E-5
72-73	0.0018425795380167245	3.5751543274951367E-4	4.5835311890963294E-6	2.7501187134577975E-5	0.1416700737581839	1.3750593567288987E-5
74-75	0.0018471630692058207	3.6209896393861E-4	4.5835311890963294E-6	2.7501187134577975E-5	0.14280908125867434	1.3750593567288987E-5
76-77	0.0018723724907458504	3.75849557505899E-4	4.5835311890963294E-6	2.7501187134577975E-5	0.14420247474015963	1.3750593567288987E-5
78-79	0.0019182078026368137	4.010589790459288E-4	4.5835311890963294E-6	2.7501187134577975E-5	0.1457242070949396	1.3750593567288987E-5
80-81	0.001920499568231362	4.1480957261321783E-4	4.5835311890963294E-6	2.7501187134577975E-5	0.14718177001307223	1.3750593567288987E-5
82-83	0.0019250830994204584	4.354354629641513E-4	4.5835311890963294E-6	2.7501187134577975E-5	0.1488639259594706	1.3750593567288987E-5
84-85	0.0019388336929877473	4.377272285586995E-4	4.5835311890963294E-6	2.7501187134577975E-5	0.1506537948888127	1.6042359161837153E-5
86-87	0.001975501942500518	4.400189941532476E-4	9.167062378192659E-6	2.7501187134577975E-5	0.15254450150431492	2.0625890350933483E-5
88-89	0.0019984195984459998	4.468942909368921E-4	1.3750593567288987E-5	2.7501187134577975E-5	0.15440312340149348	2.2917655945481648E-5
90-91	0.0020144619576078367	4.812707748551146E-4	1.3750593567288987E-5	2.7501187134577975E-5	0.15547566969974203	3.2084718323674305E-5
92-93	0.0020282125511751257	4.812707748551146E-4	2.2917655945481648E-5	2.7501187134577975E-5	0.1564817547957487	3.2084718323674305E-5
94-95	0.00203508784795877	4.858543060442109E-4	2.2917655945481648E-5	2.7501187134577975E-5	0.15756575992196997	3.6668249512770636E-5
96-97	0.002044254910336963	4.858543060442109E-4	2.2917655945481648E-5	2.7501187134577975E-5	0.1586520568137858	3.6668249512770636E-5
98-99	0.002055713738309704	4.973131340169517E-4	2.5209421540029813E-5	2.7501187134577975E-5	0.15959168070755053	3.8960015107318804E-5
100-101	0.002062589035093348	4.996048996114999E-4	2.7501187134577975E-5	2.7501187134577975E-5	0.16048776105501888	4.1251780701866966E-5
102-103	0.0020671725662824445	4.996048996114999E-4	3.2084718323674305E-5	2.7501187134577975E-5	0.16154197322851105	4.1251780701866966E-5
104-105	0.002074047863066089	5.041884308005962E-4	3.6668249512770636E-5	2.7501187134577975E-5	0.16248388888787033	4.5835311890963296E-5
106-107	0.0021084243469843113	5.179390243678852E-4	3.6668249512770636E-5	2.7501187134577975E-5	0.16327454801798943	4.812707748551146E-5
108-109	0.0021152996437679558	5.225225555569816E-4	4.5835311890963296E-5	2.7501187134577975E-5	0.16412250128797226	5.271060867460779E-5
110-111	0.0021267584717406967	5.339813835297223E-4	5.041884308005962E-5	2.7501187134577975E-5	0.1650712922441152	5.729413986370411E-5
112-113	0.0021290502373352447	5.385649147188188E-4	5.041884308005962E-5	2.7501187134577975E-5	0.16588716079577437	6.416943664734861E-5
114-115	0.0021428008309025337	5.500237426915595E-4	5.041884308005962E-5	2.7501187134577975E-5	0.16674886465932445	6.416943664734861E-5
116-117	0.002145092596497082	5.591908050697522E-4	5.729413986370411E-5	2.7501187134577975E-5	0.16771598974022378	6.416943664734861E-5
118-119	0.0021496761276861786	5.591908050697522E-4	5.958590545825228E-5	2.7501187134577975E-5	0.16856165124461206	7.792003021463761E-5
120-121	0.0021496761276861786	5.683578674479449E-4	5.958590545825228E-5	2.7501187134577975E-5	0.16946460688886403	8.708709259283026E-5
122-123	0.0021542596588752746	5.729413986370411E-4	5.958590545825228E-5	2.7501187134577975E-5	0.17029193426849593	8.708709259283026E-5
124-125	0.00217259378363166	5.798166954206856E-4	5.958590545825228E-5	2.979295272912614E-5	0.1712567675838007	9.167062378192659E-5
126-127	0.0021886361427934974	5.866919922043302E-4	5.958590545825228E-5	3.2084718323674305E-5	0.1722376432582673	9.625415497102292E-5
128-129	0.0022069702675498824	5.889837577988783E-4	5.958590545825228E-5	3.2084718323674305E-5	0.17319330951119388	9.625415497102292E-5
130-131	0.0022115537987389793	6.027343513661672E-4	5.958590545825228E-5	3.2084718323674305E-5	0.1742589805126588	1.0542121734921558E-4
132-133	0.0022275961579008158	6.141931793389082E-4	6.187767105280045E-5	3.2084718323674305E-5	0.17516193615691078	1.0771298294376374E-4
134-135	0.0022459302826572016	6.187767105280044E-4	6.416943664734861E-5	3.2084718323674305E-5	0.17608551769151368	1.100047485383119E-4
136-137	0.002252805579440846	6.233602417171008E-4	6.416943664734861E-5	3.2084718323674305E-5	0.177052642772413	1.1458827972740824E-4
138-139	0.002255097345035394	6.41694366473486E-4	6.416943664734861E-5	3.2084718323674305E-5	0.17785705249609943	1.1458827972740824E-4
>>END_MODULE
