##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.4d.R.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23004401
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	64-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.03088600307393	37.0	35.0	38.0	33.0	39.0
2	35.40721981850343	35.0	34.0	37.0	33.0	39.0
3	35.517640124600504	35.0	34.0	37.0	33.0	39.0
4	35.61572009634156	36.0	35.0	38.0	33.0	39.0
5	35.54278852989913	36.0	35.0	37.0	33.0	39.0
6	35.47568758691	36.0	35.0	37.0	33.0	39.0
7	35.439411702134734	35.0	34.0	37.0	33.0	39.0
8	35.59346018181478	36.0	35.0	38.0	33.0	39.0
9	35.56768041906416	36.0	35.0	38.0	33.0	39.0
10-14	35.49342591445872	36.0	34.8	37.8	33.0	39.0
15-19	35.49770841675034	36.0	34.8	38.0	33.0	39.0
20-24	35.47400250934593	36.0	34.0	38.0	33.0	39.0
25-29	35.455427037635104	36.0	34.0	38.0	33.0	39.0
30-34	35.42375606302464	36.0	34.0	38.0	33.0	39.0
35-39	35.39876120225865	36.0	34.0	38.0	33.0	39.0
40-44	35.37573175671907	36.0	34.0	38.0	33.0	39.0
45-49	35.338659424342325	36.0	34.0	38.0	32.0	39.0
50-54	35.312777194242095	36.0	34.0	38.0	32.0	39.0
55-59	35.276455161775345	36.0	34.0	38.0	32.0	39.0
60-64	35.23261814119828	36.0	34.0	37.8	32.0	39.0
65-69	35.188633960429016	36.0	34.0	37.2	32.0	39.0
70-74	35.152772578075414	36.0	34.0	37.0	32.0	39.0
75-79	35.101284846939066	35.6	34.0	37.0	32.0	39.0
80-84	35.055404776731436	35.4	34.0	37.0	32.0	39.0
85-89	35.00395782234527	35.0	34.0	37.0	31.8	39.0
90-94	34.94062408625133	35.0	34.0	37.0	31.0	39.0
95-99	34.86915794524232	35.0	34.0	37.0	31.0	39.0
100-104	34.808635696630176	35.0	34.0	37.0	31.0	39.0
105-109	34.73419648721268	35.0	34.0	37.0	31.0	39.0
110-114	34.66174049037105	35.0	34.0	37.0	30.2	39.0
115-119	34.5824677901375	35.0	34.0	37.0	30.0	39.0
120-124	34.497067087358474	35.0	34.0	37.0	30.0	39.0
125-129	34.42389345173512	35.0	34.0	37.0	30.0	39.0
130-134	34.32117726231378	35.0	34.0	37.0	29.8	39.0
135-139	34.217806468821756	35.0	34.0	37.0	29.0	39.0
140-144	34.112447217644174	35.0	34.0	37.0	29.0	39.0
145-149	34.080895623172935	35.0	34.0	37.0	29.0	39.0
150	34.87878702201421	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	133.0
22	1275.0
23	7876.0
24	28539.0
25	73277.0
26	145303.0
27	248319.0
28	360895.0
29	470370.0
30	604788.0
31	785167.0
32	1059477.0
33	1509952.0
34	2341373.0
35	4138375.0
36	9906870.0
37	1322399.0
38	5.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.883246992608065	19.03292765588637	16.943618744952325	31.14020660655324
2	24.84647611559197	24.98756650955615	32.768677610862376	17.397279763989506
3	20.291439016386473	29.071884984094996	30.518638585721053	20.118037413797474
4	23.967381195222707	33.436752683693236	22.51802361800454	20.07784250307952
5	25.660050874613077	35.042981558180976	22.371997427796533	16.92497013940941
6	19.067347156746226	39.25304118981407	22.628357069588553	19.05125458385115
7	20.45746811664429	20.987862279048255	38.019151205023775	20.535518399283685
8	21.545182660708388	23.53374571821043	28.50070421310706	26.420367407974126
9	23.62234283973252	23.258503732264423	28.836258665136178	24.282894762866878
10-14	23.079235538705788	29.464948034984044	25.700126170779917	21.755690255530247
15-19	23.031104352597577	28.398239102161366	27.102347937683753	21.46830860755731
20-24	23.108455638553686	28.467102447049154	27.002968692816648	21.421473221580513
25-29	23.221661802887194	28.371411192145363	26.91439172878268	21.492535276184764
30-34	23.176393073655777	28.20528471921525	27.113865733778507	21.50445647335047
35-39	23.306971566006	28.151994916103227	27.058297236254923	21.482736281635848
40-44	23.420405512840784	27.93240041329483	27.188738363585298	21.45845571027909
45-49	23.436911253954502	27.765609961029718	27.260417928381774	21.537060856634007
50-54	23.496344434203603	27.742991051894656	27.208029058579342	21.552635455322406
55-59	23.65560572518276	27.631112846624433	27.148839041712066	21.564442386480742
60-64	23.544917339947254	27.61113927721917	27.20803641007649	21.635906972757084
65-69	23.655367752786706	27.52344095654074	27.188338019249713	21.632853271422842
70-74	23.74037881824258	27.44994314689547	27.109529603080762	21.700148431781184
75-79	23.66199267457979	27.507215358331504	27.200316642966925	21.630475324121775
80-84	23.786555088887113	27.557805003109472	27.034267735045674	21.62137217295774
85-89	23.791022589283457	27.515443990100195	27.110033544463867	21.58349987615248
90-94	23.7439009034389	27.44240576900106	27.1469457614192	21.666747566140838
95-99	23.779915491100592	27.56253995634707	27.161316523875467	21.49622802867687
100-104	23.98252809935632	27.496849882061586	26.98492338448019	21.53569863410191
105-109	23.737132741627594	27.636701188317296	27.115612888638974	21.510553181416135
110-114	23.7895390329918	27.624834797548743	27.02811919617704	21.55750697328242
115-119	23.840094551122544	27.634310842206318	27.065818792046038	21.459775814625097
120-124	23.795137615349798	27.54142761763381	27.231316933182114	21.432117833834276
125-129	23.775001239361305	27.642492876161633	27.19039230591978	21.392113578557275
130-134	23.84397389100334	27.556317503738093	27.263229597802557	21.33647900745601
135-139	23.72116631470716	27.5573288267209	27.382006156584342	21.339498701987598
140-144	23.7456888091049	27.679385991792604	27.334700434804542	21.240224764297956
145-149	23.495594212953687	27.876575397833808	27.205438049708363	21.422392339504135
150	23.662647790664668	26.63188864729782	27.583311883904155	22.122151678133363
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.5
2	12.0
3	30.0
4	40.0
5	43.5
6	42.5
7	34.0
8	29.0
9	25.5
10	16.5
11	14.5
12	21.5
13	22.0
14	24.5
15	44.0
16	81.5
17	150.5
18	239.0
19	423.5
20	701.5
21	1098.0
22	1720.0
23	2820.0
24	4658.0
25	7565.5
26	11681.0
27	18138.0
28	29065.0
29	43495.5
30	58568.0
31	76699.0
32	102513.5
33	137074.5
34	181251.0
35	246311.0
36	343370.0
37	461763.0
38	600886.5
39	777793.5
40	986389.5
41	1179732.0
42	1348031.5
43	1502845.5
44	1614808.0
45	1663872.5
46	1656322.5
47	1599685.0
48	1499236.5
49	1357505.5
50	1179598.0
51	991578.5
52	810091.0
53	643157.0
54	500576.5
55	378527.0
56	279293.5
57	207946.0
58	152896.5
59	109015.5
60	75386.5
61	50036.0
62	33760.5
63	22985.0
64	15276.5
65	10422.0
66	7232.0
67	5354.5
68	4691.5
69	3559.0
70	2211.0
71	1535.0
72	821.5
73	566.5
74	486.5
75	321.5
76	211.0
77	76.0
78	28.5
79	3.5
80	0.5
81	0.5
82	0.5
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	8.693988598094774E-6
5	0.0
6	0.0
7	0.0
8	4.346994299047387E-6
9	8.693988598094774E-6
10-14	0.0952017833457172
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	8.693988598094773E-7
50-54	1.7387977196189546E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	1.7388028896782688E-6
80-84	8.694047177203721E-7
85-89	0.0
90-94	8.694193817402281E-7
95-99	6.9554501764280365E-6
100-104	0.0
105-109	0.0
110-114	0.0
115-119	8.695091605137735E-7
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
64-65	6.0
66-67	3.0
68-69	9.0
70-71	10.0
72-73	13.0
74-75	15.0
76-77	23.0
78-79	27.0
80-81	47.0
82-83	59.0
84-85	52.0
86-87	78.0
88-89	92.0
90-91	106.0
92-93	113.0
94-95	123.0
96-97	152.0
98-99	168.0
100-101	207.0
102-103	178.0
104-105	183.0
106-107	220.0
108-109	229.0
110-111	203.0
112-113	220.0
114-115	252.0
116-117	256.0
118-119	284.0
120-121	305.0
122-123	350.0
124-125	433.0
126-127	459.0
128-129	517.0
130-131	597.0
132-133	830.0
134-135	1083.0
136-137	1650.0
138-139	3450.0
140-141	6986.0
142-143	10633.0
144-145	65328.0
146-147	794743.0
148-149	963185.0
150-151	2.1150524E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.50737014422417
#Duplication Level	Percentage of total
1	26.346108415144126
2	11.402899163196075
3	7.609360990217364
4	5.367482868017963
5	4.165126508719341
6	3.243634185638452
7	2.705529922328155
8	2.2195810737953177
9	1.954490121410946
>10	21.891460906338725
>50	5.375716209126594
>100	5.986624533755074
>500	0.8597253938386044
>1k	0.7844668951815367
>5k	0.08779281329166998
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.346994299047386E-6	1.304098289714216E-5	0.0	4.346994299047386E-6	0.0016214288735446752	0.0
2	2.1734971495236934E-5	5.651092588761603E-5	0.0	4.346994299047386E-6	0.005625010622967318	0.0
3	5.216393158856864E-5	5.651092588761603E-5	0.0	4.346994299047386E-6	0.00718558157632533	0.0
4	1.0432786317713728E-4	5.651092588761603E-5	0.0	4.346994299047386E-6	0.008541843797628115	0.0
5	1.0867485747618467E-4	5.651092588761603E-5	0.0	4.346994299047386E-6	0.0102284775856585	0.0
6	1.1302185177523205E-4	5.651092588761603E-5	0.0	4.346994299047386E-6	0.01181078351051175	0.0
7	1.2171584037332683E-4	6.52049144857108E-5	0.0	4.346994299047386E-6	0.013745195973587837	0.0
8	1.4779780616761115E-4	6.955190878475818E-5	0.0	4.346994299047386E-6	0.01915720387590183	0.0
9	1.5649179476570591E-4	7.824589738285296E-5	0.0	4.346994299047386E-6	0.025056075139709137	0.0
10-11	1.7605326911141915E-4	9.128688027999512E-5	0.0	4.346994299047386E-6	0.03166567997141069	0.0
12-13	2.0648222920475087E-4	9.128688027999512E-5	0.0	4.346994299047386E-6	0.03735372201171419	2.173497149523693E-6
14-15	2.47778675045701E-4	9.346037742951881E-5	0.0	4.346994299047386E-6	0.04535436501911091	4.346994299047386E-6
16-17	2.7168714369046166E-4	9.99808688780899E-5	0.0	4.346994299047386E-6	0.05101197809932108	4.346994299047386E-6
18-19	3.0211610378379336E-4	1.0650136032666098E-4	0.0	4.346994299047386E-6	0.05875397494592448	4.346994299047386E-6
20-21	3.390655553256962E-4	1.1302185177523205E-4	0.0	4.346994299047386E-6	0.06464849921543273	4.346994299047386E-6
22-23	3.8036200116664634E-4	1.2823633182189792E-4	0.0	4.346994299047386E-6	0.07046477758755815	6.52049144857108E-6
24-25	4.2383194415712016E-4	1.3475682327046898E-4	0.0	4.346994299047386E-6	0.07562683331767692	8.693988598094772E-6
26-27	4.629548928485467E-4	1.3910381756951636E-4	8.693988598094772E-6	4.346994299047386E-6	0.07955216916971669	8.693988598094772E-6
28-29	5.477212816799707E-4	1.4127731471904006E-4	8.693988598094772E-6	4.346994299047386E-6	0.08363834381082125	8.693988598094772E-6
30-31	6.585696363056791E-4	1.4997130331713485E-4	1.304098289714216E-5	4.346994299047386E-6	0.08843090502552098	8.693988598094772E-6
32-33	7.781119795294822E-4	1.63012286214277E-4	1.7387977196189545E-5	4.346994299047386E-6	0.0932930181490055	8.693988598094772E-6
34-35	8.6505186551043E-4	1.7387977196189547E-4	1.7387977196189545E-5	4.346994299047386E-6	0.09631852618114248	8.693988598094772E-6
36-37	9.259097856970933E-4	1.7822676626094285E-4	1.7387977196189545E-5	4.346994299047386E-6	0.09993739893509943	8.693988598094772E-6
38-39	9.715532258370909E-4	1.8257376055999023E-4	1.7387977196189545E-5	4.346994299047386E-6	0.1032193796308802	8.693988598094772E-6
40-41	0.0010106761745285174	1.9344124630760873E-4	1.7387977196189545E-5	4.346994299047386E-6	0.10594277155923337	8.693988598094772E-6
42-43	0.001021543660276136	2.021352349057035E-4	1.7387977196189545E-5	4.346994299047386E-6	0.10866398999043705	8.693988598094772E-6
44-45	0.0010432786317713728	2.1300272065332196E-4	1.7387977196189545E-5	4.346994299047386E-6	0.11090921254589503	8.693988598094772E-6
46-47	0.0010889220719113705	2.4343168074665366E-4	1.7387977196189545E-5	4.346994299047386E-6	0.11350002114812727	1.5214480046665852E-5
48-49	0.0011367390092008917	2.47778675045701E-4	1.7387977196189545E-5	4.346994299047386E-6	0.11569960026344525	1.7387977196189545E-5
50-51	0.0011889029407894602	2.5647266364379584E-4	1.7387977196189545E-5	4.346994299047386E-6	0.1177144321210537	1.7387977196189545E-5
52-53	0.0012236788951818394	2.5647266364379584E-4	1.7387977196189545E-5	4.346994299047386E-6	0.1199074907449231	1.7387977196189545E-5
54-55	0.0012801898210694554	2.608196579428432E-4	1.7387977196189545E-5	4.346994299047386E-6	0.12184842369944777	1.7387977196189545E-5
56-57	0.0013301802555085005	2.651666522418906E-4	1.7387977196189545E-5	4.346994299047386E-6	0.12394584844873813	1.956147434571324E-5
58-59	0.0013779971927980216	2.7386064083998536E-4	1.7387977196189545E-5	4.346994299047386E-6	0.1258498319517209	2.1734971495236934E-5
60-61	0.0014366816158351613	2.955956123352223E-4	1.7387977196189545E-5	4.346994299047386E-6	0.12737127995638747	2.608196579428432E-5
62-63	0.0014844985531246827	3.0646309808284077E-4	1.7387977196189545E-5	4.346994299047386E-6	0.12930786591661309	3.477595439237909E-5
64-65	0.0015062335246199194	3.3471856102664877E-4	1.7387977196189545E-5	1.0867485747618465E-5	0.13111838904216633	4.129644584095017E-5
66-67	0.001623602370694199	3.608005268209331E-4	1.956147434571324E-5	2.173497149523693E-5	0.1324855187492167	5.216393158856864E-5
68-69	0.0016562048279370543	3.694945154190279E-4	2.1734971495236934E-5	2.608196579428432E-5	0.1338591689477157	6.52049144857108E-5
70-71	0.0016648988165351491	3.7384150971807523E-4	2.1734971495236934E-5	2.608196579428432E-5	0.13521977816331754	6.955190878475818E-5
72-73	0.0016822867937313388	3.999234755123596E-4	2.1734971495236934E-5	2.8255462943808013E-5	0.13615655543476224	6.955190878475818E-5
74-75	0.0017040217652265755	4.173114527085491E-4	2.1734971495236934E-5	3.26024572428554E-5	0.13727373296961742	6.955190878475818E-5
76-77	0.0017192362452732412	4.4339341850283345E-4	2.1734971495236934E-5	3.477595439237909E-5	0.13848654437905167	6.955190878475818E-5
78-79	0.0017800941654599048	4.825163671942599E-4	2.1734971495236934E-5	3.477595439237909E-5	0.13994713446353158	6.955190878475818E-5
80-81	0.0018040026341046656	4.933838529418784E-4	2.608196579428432E-5	3.477595439237909E-5	0.14142945951950672	6.955190878475818E-5
82-83	0.001834431594197997	5.129453272875917E-4	2.8255462943808013E-5	3.477595439237909E-5	0.14308566434744377	6.955190878475818E-5
84-85	0.0018626870571418052	5.194658187361626E-4	3.0428960093331708E-5	3.694945154190279E-5	0.1447223077010351	7.172540593428187E-5
86-87	0.0018757280400389473	5.259863101847338E-4	3.26024572428554E-5	3.912294869142648E-5	0.14666106715841026	7.389890308380558E-5
88-89	0.0018996365086837081	5.281598073342574E-4	3.477595439237909E-5	3.912294869142648E-5	0.1483911708894311	7.389890308380558E-5
90-91	0.0019039835029827554	5.564152702780654E-4	3.477595439237909E-5	4.346994299047387E-5	0.14945401099554823	7.389890308380558E-5
92-93	0.0019039835029827554	5.564152702780654E-4	3.477595439237909E-5	4.346994299047387E-5	0.15041469673563768	7.389890308380558E-5
94-95	0.001906157000132279	5.651092588761603E-4	3.477595439237909E-5	4.346994299047387E-5	0.15149927181325	7.824589738285296E-5
96-97	0.00191267749158085	5.738032474742551E-4	3.477595439237909E-5	4.346994299047387E-5	0.15259688787375947	7.824589738285296E-5
98-99	0.00191267749158085	5.824972360723498E-4	3.477595439237909E-5	4.346994299047387E-5	0.1535749615910451	7.824589738285296E-5
100-101	0.00191267749158085	5.846707332218734E-4	3.477595439237909E-5	4.346994299047387E-5	0.15442262547935937	7.824589738285296E-5
102-103	0.00191267749158085	5.911912246704446E-4	3.912294869142648E-5	4.346994299047387E-5	0.15543112815673837	8.041939453237666E-5
104-105	0.0019213714801789448	5.911912246704446E-4	4.346994299047387E-5	4.346994299047387E-5	0.1564265898512202	8.476638883142404E-5
106-107	0.0019518004402722766	6.085792018666341E-4	4.346994299047387E-5	4.346994299047387E-5	0.15728294772813256	9.128688027999512E-5
108-109	0.0019518004402722766	6.237936819133E-4	4.346994299047387E-5	4.346994299047387E-5	0.15801976326182107	9.346037742951881E-5
110-111	0.001962667926019895	6.411816591094895E-4	5.216393158856864E-5	4.346994299047387E-5	0.15887394764158388	9.563387457904251E-5
112-113	0.001975708908917037	6.498756477075842E-4	5.216393158856864E-5	4.346994299047387E-5	0.15963467164391718	1.0215436602761359E-4
114-115	0.0019800559032160845	6.52049144857108E-4	5.216393158856864E-5	4.346994299047387E-5	0.16055840793246476	1.0432786317713728E-4
116-117	0.0019887498918141795	6.52049144857108E-4	5.216393158856864E-5	4.346994299047387E-5	0.1615560431240961	1.0867485747618466E-4
118-119	0.0019996173775617978	6.585696363056791E-4	5.651092588761603E-5	4.346994299047387E-5	0.1624167479953075	1.1302185177523205E-4
120-121	0.002003964371860845	6.607431334552028E-4	5.651092588761603E-5	4.346994299047387E-5	0.1633144023180608	1.1736884607427945E-4
122-123	0.0020104848633094165	6.629166306047265E-4	5.651092588761603E-5	4.346994299047387E-5	0.16417510718927217	1.238893375228505E-4
124-125	0.0020213523490570347	6.694371220532975E-4	5.651092588761603E-5	4.346994299047387E-5	0.16516187489515594	1.260628346723742E-4
126-127	0.002043087320552272	6.80304607800916E-4	5.651092588761603E-5	4.346994299047387E-5	0.16613560161814256	1.304098289714216E-4
128-129	0.0020648222920475086	6.998660821466293E-4	5.651092588761603E-5	4.346994299047387E-5	0.1670941138610825	1.304098289714216E-4
130-131	0.0020800367720941746	7.259480479409135E-4	5.8684423037139725E-5	4.7816937289521256E-5	0.16821998538453575	1.3475682327046898E-4
132-133	0.0020909042578417933	7.346420365390083E-4	6.0857920186663416E-5	4.7816937289521256E-5	0.16908069025574712	1.4345081186856377E-4
134-135	0.0021235067150846484	7.411625279875794E-4	6.30314173361871E-5	4.7816937289521256E-5	0.1698827107039214	1.4562430901808745E-4
136-137	0.0021278537093836957	7.542035108847216E-4	6.52049144857108E-5	4.7816937289521256E-5	0.17061517924331088	1.4779780616761115E-4
138-139	0.0021300272065332194	7.585505051837689E-4	6.52049144857108E-5	4.7816937289521256E-5	0.17134764778270037	1.5649179476570591E-4
>>END_MODULE
