##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.4d.R.3.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24076573
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	66-150
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.38567141594445	36.0	34.0	38.0	31.0	39.0
2	34.84292789509537	35.0	34.0	38.0	31.0	39.0
3	34.97550444575314	35.0	34.0	38.0	31.0	39.0
4	34.98225146078721	36.0	34.0	38.0	31.0	39.0
5	34.89795474630048	35.0	34.0	37.0	31.0	39.0
6	34.85048586441268	35.0	34.0	37.0	32.0	39.0
7	34.801357942428105	35.0	34.0	37.0	32.0	39.0
8	34.94582019625467	35.0	34.0	37.0	32.0	39.0
9	34.92295398518718	35.0	34.0	37.0	32.0	39.0
10-14	34.93720901226267	35.2	34.0	37.0	31.6	39.0
15-19	34.93649377758206	35.0	34.0	37.0	31.8	39.0
20-24	34.932523328797664	35.0	34.0	37.0	31.4	39.0
25-29	34.920268071373776	35.0	34.0	37.0	31.0	39.0
30-34	34.9030336335657	35.0	34.0	37.0	31.0	39.0
35-39	34.89589540006379	35.0	34.0	37.0	31.0	39.0
40-44	34.83960449853058	35.0	34.0	37.0	31.0	39.0
45-49	34.75564376209189	35.0	34.0	37.0	31.0	39.0
50-54	34.73586438568313	35.0	34.0	37.0	31.0	39.0
55-59	34.71710541197039	35.0	34.0	37.0	31.0	39.0
60-64	34.70795502333326	35.0	34.0	37.0	31.0	39.0
65-69	34.68713397086939	35.0	34.0	37.0	31.0	39.0
70-74	34.67720505045452	35.0	34.0	37.0	31.0	39.0
75-79	34.65499973609742	35.0	34.0	37.0	31.0	39.0
80-84	34.645345067958296	35.0	34.0	37.0	31.0	39.0
85-89	34.628257800533405	35.0	34.0	37.0	31.0	39.0
90-94	34.61474874792344	35.0	34.0	37.0	31.0	39.0
95-99	34.59482824522916	35.0	34.0	37.0	30.8	39.0
100-104	34.55995885273097	35.0	34.0	37.0	30.0	39.0
105-109	34.53721839635823	35.0	34.0	37.0	30.0	39.0
110-114	34.49885463208721	35.0	34.0	37.0	30.0	39.0
115-119	34.476467593994485	35.0	34.0	37.0	30.0	39.0
120-124	34.45281773034335	35.0	34.0	37.0	30.0	39.0
125-129	34.42648512027053	35.0	34.0	37.0	30.0	39.0
130-134	34.3900166792517	35.0	34.0	37.0	30.0	39.0
135-139	34.36442916720931	35.0	34.0	37.0	30.0	39.0
140-144	34.32817155579183	35.0	34.0	37.0	30.0	39.0
145-149	34.35542520403598	35.0	34.0	37.0	30.0	39.0
150	35.11060467478869	35.0	34.0	37.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	4.0
22	171.0
23	2164.0
24	16533.0
25	71487.0
26	189280.0
27	345471.0
28	507201.0
29	657767.0
30	819885.0
31	1037314.0
32	1345416.0
33	1834972.0
34	2675494.0
35	4478339.0
36	9019434.0
37	1075635.0
38	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.38284705219468	10.966145389545265	15.758638075277572	37.89236948298249
2	22.93712701007266	12.42822322379593	35.68020424848987	28.954445517641542
3	21.68218328235982	17.44448001616178	28.143742817701998	32.7295938837764
4	26.561097247225234	24.829980782540083	22.1734947579476	26.435427212287077
5	26.146214980652545	30.66714962626702	24.23912265163594	18.9475127414445
6	19.499706498610454	36.56530980127673	25.464195509430816	18.470788190681994
7	16.071464098013763	25.275690602847078	40.17318603584893	18.479659263290234
8	18.551030149217627	24.508595763373034	31.482068498958654	25.458305588450685
9	19.71733649635664	23.142828270653307	32.58847833760852	24.551356895381534
10-14	20.240816590077973	29.36553325502803	27.146515038175806	23.247135116718187
15-19	20.46528990761989	27.94058218900429	27.93868075180019	23.65544715157563
20-24	20.617742048282732	28.111260199523507	27.687695210243113	23.583302541950648
25-29	20.622542868993417	28.294867657739115	27.546689273935378	23.535900199332094
30-34	20.572440865005166	28.04874495063047	27.58872155650451	23.79009262785985
35-39	20.6384542891118	27.986894236396342	27.658051463609844	23.71660001088201
40-44	20.6761596911606	28.059848332858294	27.57217805820524	23.69181391777587
45-49	20.775170273972048	27.847546484060665	27.536750761067157	23.84053248090013
50-54	20.907191568098014	27.822613872603196	27.60609887702544	23.664095682273352
55-59	20.877379812825996	27.854023678864376	27.56085328189982	23.707743226409807
60-64	20.836029439733288	27.786161234011754	27.530340453994867	23.84746887226009
65-69	20.92012942972534	27.705253328152946	27.628869135993302	23.745748106128413
70-74	21.071721815129308	27.620590470227896	27.59920614417217	23.70848157047062
75-79	21.096044499963128	27.546184488148434	27.588444022278292	23.76932698961015
80-84	21.136455157163205	27.533199580194694	27.54880899562196	23.781536267020137
85-89	21.08886609373533	27.557056263264716	27.63251917553271	23.721558467467236
90-94	21.080208422710214	27.479391596837544	27.56288076523813	23.87751921521411
95-99	21.173558350147427	27.386921067141788	27.666258511960308	23.773262070750473
100-104	21.240912190638923	27.439571072153228	27.522229980615204	23.79728675659264
105-109	21.19497673711702	27.290581262474245	27.642052729267874	23.87238927114086
110-114	21.272317563602446	27.259778282819774	27.692212877760152	23.775691275817625
115-119	21.28454088192955	27.28781871690072	27.67992847719667	23.74771192397306
120-124	21.26277475325062	27.177937215816563	27.69433898914092	23.8649490417919
125-129	21.31032719751036	27.10117809007311	27.739937714774758	23.848556997641772
130-134	21.37440085168785	27.207252924131858	27.646085265298925	23.77226095888137
135-139	21.35994919077841	27.08100357350992	27.74442446469004	23.81462277102163
140-144	21.392934692866408	26.96981172748939	27.91246237819894	23.72479120144526
145-149	21.050841511581606	27.265813785555725	27.88980971452525	23.793534988337417
150	21.181930784199686	25.82972662090222	28.387305067039197	24.601037527858892
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	1.0
9	0.0
10	1.5
11	6.0
12	12.0
13	18.0
14	39.0
15	86.0
16	168.0
17	332.5
18	660.5
19	1294.0
20	2586.5
21	4337.0
22	6576.5
23	10194.5
24	15963.0
25	24666.0
26	36025.0
27	50121.5
28	67655.0
29	93326.5
30	125620.5
31	159464.0
32	193862.0
33	232019.5
34	272668.5
35	320136.0
36	392217.0
37	489453.5
38	608314.0
39	755376.0
40	923478.5
41	1090559.0
42	1256918.5
43	1428231.5
44	1568660.5
45	1650968.5
46	1669567.0
47	1627151.5
48	1540054.5
49	1411079.5
50	1244660.5
51	1067162.0
52	888300.5
53	713824.0
54	556003.0
55	420176.0
56	313928.0
57	234408.5
58	175695.0
59	130072.0
60	91655.5
61	64085.0
62	46284.5
63	30971.0
64	19729.5
65	14372.5
66	12339.0
67	9796.0
68	5552.5
69	2682.0
70	2245.5
71	1570.5
72	678.0
73	244.0
74	107.5
75	66.5
76	46.0
77	37.0
78	15.0
79	0.0
80	0.0
81	1.0
82	1.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	7.476147041358418E-5
3	7.060805539060729E-5
4	1.2460245068930697E-4
5	1.0383537557442248E-4
6	1.0798879059739939E-4
7	1.4952294082716837E-4
8	2.533583164015909E-4
9	1.2460245068930697E-4
10-14	1.7610479697422054E-4
15-19	2.0019460410748658E-4
20-24	1.3124791472607003E-4
25-29	2.857549535808107E-4
30-34	4.228176493390484E-4
35-39	5.939383482856966E-4
40-44	2.467128523648278E-4
45-49	2.1265484917641725E-4
50-54	3.663312050265625E-4
55-59	5.449280510145693E-4
60-64	7.741965602828941E-4
65-69	5.183462077849586E-4
70-74	2.3757536891675184E-4
75-79	4.31124636961282E-4
80-84	2.4837441437840316E-4
85-89	3.015384015758347E-4
90-94	3.9208338782973994E-4
95-99	5.690204058692137E-4
100-104	3.9540816485463535E-4
105-109	4.5604981100639587E-4
110-114	2.832670835865258E-4
115-119	3.5969280141396985E-4
120-124	3.0569938540883043E-4
125-129	3.655135111363286E-4
130-134	3.0321441673732943E-4
135-139	2.957493360219717E-4
140-144	2.6759118701555817E-4
145-149	2.8261563375193565E-4
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
66-67	1.0
68-69	0.0
70-71	1.0
72-73	4.0
74-75	1.0
76-77	4.0
78-79	3.0
80-81	6.0
82-83	3.0
84-85	10.0
86-87	9.0
88-89	10.0
90-91	8.0
92-93	15.0
94-95	21.0
96-97	22.0
98-99	37.0
100-101	32.0
102-103	25.0
104-105	25.0
106-107	36.0
108-109	36.0
110-111	50.0
112-113	34.0
114-115	48.0
116-117	61.0
118-119	65.0
120-121	73.0
122-123	54.0
124-125	98.0
126-127	120.0
128-129	124.0
130-131	145.0
132-133	215.0
134-135	335.0
136-137	614.0
138-139	2112.0
140-141	5043.0
142-143	7517.0
144-145	67077.0
146-147	751731.0
148-149	1171943.0
150-151	2.2068805E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.83198904731974
#Duplication Level	Percentage of total
1	36.64792580377441
2	11.247507798430956
3	6.753204893256297
4	4.931973874442323
5	3.535967270163271
6	2.7972944691122517
7	2.3418733967055037
8	1.9334443966452293
9	1.6783891954510295
>10	18.861628882960648
>50	4.4582468835466775
>100	4.21148315155563
>500	0.3362315169585135
>1k	0.2648284669971023
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.2460245068930699E-5	0.0	4.1534150229769E-6	1.9521050607991427E-4	2.4920490137861398E-5
2	4.1534150229769E-6	1.1214220562037629E-4	0.0	1.66136600919076E-5	0.001698746744397552	4.153415022976899E-5
3	4.1534150229769E-6	1.24602450689307E-4	0.0	1.66136600919076E-5	0.002890776855991922	7.891488543656109E-5
4	2.9073905160838295E-5	1.4121611078121458E-4	0.0	2.0767075114884496E-5	0.004224023078367507	1.0383537557442248E-4
5	3.32273201838152E-5	1.495229408271684E-4	0.0	2.0767075114884496E-5	0.005499121490421415	1.0798879059739939E-4
6	5.3994395298699696E-5	1.495229408271684E-4	0.0	2.0767075114884496E-5	0.006649617451786017	1.1214220562037629E-4
7	7.060805539060729E-5	1.8275026101098358E-4	0.0	2.0767075114884496E-5	0.007858261223472295	1.1214220562037629E-4
8	8.722171548251489E-5	1.8275026101098358E-4	0.0	2.0767075114884496E-5	0.010013883620397304	1.24602450689307E-4
9	1.1214220562037629E-4	1.8275026101098358E-4	0.0	2.0767075114884496E-5	0.013062490247262349	1.329092807352608E-4
10-11	1.7236672345354135E-4	1.8898038354544892E-4	0.0	2.2843782626372947E-5	0.017649937140140334	1.7029001594205288E-4
12-13	2.2220770372926413E-4	1.910570910569374E-4	0.0	2.4920490137861398E-5	0.021442005056118242	2.0767075114884497E-4
14-15	2.388213638211717E-4	2.0559404363735653E-4	0.0	2.4920490137861398E-5	0.025778170340106127	2.3466794879819483E-4
16-17	2.6997197649349845E-4	2.2428441124075257E-4	0.0	2.4920490137861398E-5	0.03019532721704206	2.533583164015909E-4
18-19	3.09429419211779E-4	2.49204901378614E-4	0.0	2.4920490137861398E-5	0.03392509390767531	2.533583164015909E-4
20-21	3.488868619300595E-4	2.762020990279638E-4	0.0	2.6997197649349848E-5	0.037638246938216664	2.6166514644754464E-4
22-23	4.1534150229768994E-4	2.7827880653945227E-4	0.0	3.32273201838152E-5	0.04167536634055021	2.7412539151647534E-4
24-25	4.8179614266532036E-4	2.7827880653945227E-4	0.0	3.32273201838152E-5	0.046163131272876745	2.7827880653945227E-4
26-27	5.420206604984854E-4	2.7827880653945227E-4	0.0	3.73807352067921E-5	0.05024178482544007	2.9073905160838297E-4
28-29	5.877082257512312E-4	2.8035551405094074E-4	0.0	3.73807352067921E-5	0.0548749192835708	3.011225891658252E-4
30-31	6.458560360729079E-4	2.845089290739176E-4	0.0	3.73807352067921E-5	0.05956412484451172	3.239663717921981E-4
32-33	7.102339689290498E-4	2.865856365854061E-4	0.0	4.361085774125744E-5	0.06362616473698313	3.5927039948750186E-4
34-35	7.434612891128651E-4	2.865856365854061E-4	0.0	4.5687565252745894E-5	0.06729363020227172	3.696539370449441E-4
36-37	7.600749492047726E-4	2.865856365854061E-4	0.0	4.776427276423435E-5	0.07092371493235353	3.7796076709089785E-4
38-39	7.663050717392379E-4	2.9073905160838297E-4	0.0	4.9840980275722795E-5	0.07529933765905969	3.9042101215982854E-4
40-41	8.07839221969007E-4	2.9489246663135985E-4	0.0	4.9840980275722795E-5	0.0788255870135671	3.9042101215982854E-4
42-43	8.182227595264492E-4	3.0527600418880213E-4	0.0	4.9840980275722795E-5	0.08223138733240815	3.9042101215982854E-4
44-45	8.472966646872875E-4	3.1565954174624436E-4	0.0	4.9840980275722795E-5	0.08649486785349393	3.9872784220578236E-4
46-47	9.033677674974757E-4	3.32273201838152E-4	0.0	4.9840980275722795E-5	0.09055898445347682	4.008045497172708E-4
48-49	9.303649651468255E-4	3.364266168611289E-4	0.0	4.9840980275722795E-5	0.09433651541687432	4.070346722517362E-4
50-51	9.407485027042677E-4	3.385033243726173E-4	0.0	4.9840980275722795E-5	0.09865606704077029	4.174182098091784E-4
52-53	9.947428980029674E-4	3.4058003188410576E-4	0.0	5.3994395298699696E-5	0.1028634764590459	4.194949173206669E-4
54-55	0.0010404304632557135	3.4058003188410576E-4	0.0	5.814781032167659E-5	0.10663270059239743	4.2157162483215534E-4
56-57	0.0010736577834395285	3.426567393955942E-4	0.0	6.0224517833165045E-5	0.11104570405431038	4.2364833234364376E-4
58-59	0.0010965015660659016	3.571936919760134E-4	0.0	6.64546403676304E-5	0.11506620979655202	4.2364833234364376E-4
60-61	0.0011671096214565088	3.675772295334556E-4	0.0	6.64546403676304E-5	0.11842839925765183	4.2364833234364376E-4
62-63	0.0011899534040828818	3.8626759713685166E-4	0.0	7.060805539060729E-5	0.12190273092437201	4.2364833234364376E-4
64-65	0.0012294108468011624	3.8626759713685166E-4	0.0	7.891488543656109E-5	0.12676637991627795	4.2364833234364376E-4
66-67	0.0012564080444505122	4.049579647402477E-4	0.0	8.306830045953799E-5	0.1311087753228003	4.2572503985513217E-4
68-69	0.0013062490247262347	4.3403186990108604E-4	0.0	8.514500797102644E-5	0.13415738194966534	4.3195516238959757E-4
70-71	0.0013747803726053536	4.547989450159705E-4	0.0	8.722171548251489E-5	0.13692563306247946	4.3610857741257445E-4
72-73	0.001408007692789169	4.6102906755043585E-4	0.0	9.55285455284687E-5	0.1399887766419249	4.5064552999299356E-4
74-75	0.0014682322106223341	4.776427276423434E-4	0.0	9.760525303995714E-5	0.14356279026919655	4.6102906755043585E-4
76-77	0.001491075993248707	4.901029727112741E-4	0.0	1.0383537557442248E-4	0.14625420320408555	4.6102906755043585E-4
78-79	0.0015346868509899642	4.942563877342511E-4	0.0	1.1837232815484164E-4	0.14889369845118738	4.6102906755043585E-4
80-81	0.0015596073411278258	4.963330952457395E-4	0.0	1.2044903566633009E-4	0.15188623397524226	4.6102906755043585E-4
82-83	0.0015907579538001525	5.025632177802048E-4	0.0	1.2044903566633009E-4	0.15456310995755085	4.6102906755043585E-4
84-85	0.0016551358866562945	5.025632177802048E-4	0.0	1.2044903566633009E-4	0.1570572356788485	4.6102906755043585E-4
86-87	0.0017361274796043442	5.067166328031818E-4	0.0	1.2044903566633009E-4	0.15924400868844582	4.6102906755043585E-4
88-89	0.0017901218749030437	5.150234628491355E-4	0.0	1.2044903566633009E-4	0.16139755437785935	4.6518248257341273E-4
90-91	0.0018088122425064398	5.254070004065779E-4	0.0	1.2044903566633009E-4	0.1640640468226105	4.7556602013085496E-4
92-93	0.0018275026101098359	5.316371229410431E-4	0.0	1.2252574317781856E-4	0.16689252245325778	4.8179614266532036E-4
94-95	0.0018877271279430009	5.378672454755084E-4	0.0	1.24602450689307E-4	0.16982483345947946	4.901029727112741E-4
96-97	0.0019521050607991426	5.420206604984854E-4	0.0	1.24602450689307E-4	0.17226081137045543	4.901029727112741E-4
98-99	0.002008176163609331	5.482507830329508E-4	0.0	1.2667915820079544E-4	0.17421914655378903	4.921796802227626E-4
100-101	0.0020580171438850537	5.524041980559276E-4	0.0	1.2875586571228388E-4	0.17638515248827147	4.963330952457395E-4
102-103	0.002080860926511427	5.524041980559276E-4	0.0	1.2875586571228388E-4	0.17819604143828943	5.004865102687165E-4
104-105	0.0021140882466952416	5.524041980559276E-4	0.0	1.329092807352608E-4	0.1798158732972504	5.025632177802048E-4
106-107	0.0021722360570169182	5.58634320590393E-4	0.0	1.3498598824674925E-4	0.1814543955238148	5.025632177802048E-4
108-109	0.0022366139898730604	5.648644431248583E-4	0.0	1.370626957582377E-4	0.18318429288088467	5.067166328031818E-4
110-111	0.002276071432591341	5.794013957052775E-4	0.0	1.370626957582377E-4	0.18480204803233416	5.12946755337647E-4
112-113	0.0023321425354015293	5.835548107282544E-4	0.0	1.370626957582377E-4	0.18635750195843903	5.150234628491355E-4
114-115	0.002390290345723206	5.897849332627197E-4	0.0	1.3913940326972614E-4	0.1881102430981353	5.150234628491355E-4
116-117	0.0024255943734185093	5.980917633086735E-4	0.0	1.495229408271684E-4	0.1897944528899524	5.150234628491355E-4
118-119	0.0024816654762286976	6.022451783316504E-4	0.0	1.536763558501453E-4	0.19134783010854578	5.150234628491355E-4
120-121	0.002566810484199724	6.063985933546273E-4	0.0	1.619831858960991E-4	0.19299673587266758	5.150234628491355E-4
122-123	0.002724640255072846	6.063985933546273E-4	0.0	1.619831858960991E-4	0.19440682027296824	5.150234628491355E-4
124-125	0.0028430125832276877	6.063985933546273E-4	0.0	1.619831858960991E-4	0.19595396736902715	5.17100170360624E-4
126-127	0.0028741631959000144	6.126287158890926E-4	0.0	1.619831858960991E-4	0.19750111446508606	5.254070004065779E-4
128-129	0.0029177740536412717	6.147054234005811E-4	0.0	1.7029001594205288E-4	0.19890081532782927	5.274837079180662E-4
130-131	0.0029759218639629484	6.167821309120696E-4	0.0	1.7029001594205288E-4	0.200294286068038	5.357905379640201E-4
132-133	0.0030631435794454633	6.23012253446535E-4	0.0	1.7652013847651823E-4	0.20149877642470132	5.399439529869969E-4
134-135	0.003196052860180724	6.23012253446535E-4	0.0	1.785968459880067E-4	0.20258697116072127	5.399439529869969E-4
136-137	0.0033248087258930083	6.333957910039772E-4	0.0	1.785968459880067E-4	0.20367724260425268	5.420206604984854E-4
138-139	0.0033393456784734273	6.520861586073732E-4	0.0	1.785968459880067E-4	0.20477166746280712	5.440973680099738E-4
>>END_MODULE
