##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.4d.R.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24076573
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	59-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.15715812212976	37.0	35.0	38.0	33.0	39.0
2	35.48974108566032	35.0	34.0	38.0	33.0	39.0
3	35.60171063381819	36.0	35.0	37.0	33.0	39.0
4	35.71317039181614	36.0	35.0	38.0	33.0	39.0
5	35.6385340637972	36.0	35.0	38.0	33.0	39.0
6	35.57214729023105	36.0	35.0	37.0	33.0	39.0
7	35.528561145309176	35.0	34.0	38.0	33.0	39.0
8	35.697154906555845	36.0	35.0	38.0	33.0	39.0
9	35.674198192575	36.0	35.0	38.0	33.0	39.0
10-14	35.59211807261772	36.0	35.0	37.8	33.0	39.0
15-19	35.6020199801691	36.0	35.0	38.0	33.0	39.0
20-24	35.58226190247258	36.0	35.0	38.0	33.0	39.0
25-29	35.564219716817675	36.0	35.0	38.0	33.0	39.0
30-34	35.53707184157812	36.0	35.0	38.0	33.0	39.0
35-39	35.51599587698798	36.0	35.0	38.0	33.0	39.0
40-44	35.49520357403024	36.0	35.0	38.0	33.0	39.0
45-49	35.459440236781205	36.0	34.0	38.0	33.0	39.0
50-54	35.43866592641735	36.0	34.0	38.0	33.0	39.0
55-59	35.406283253019446	36.0	34.0	38.0	33.0	39.0
60-64	35.36783689794693	36.0	34.0	38.0	32.4	39.0
65-69	35.32815453778399	36.0	34.0	38.0	32.0	39.0
70-74	35.295886751106785	36.0	34.0	38.0	32.0	39.0
75-79	35.24821153523398	36.0	34.0	38.0	32.0	39.0
80-84	35.207912532029084	36.0	34.0	38.0	32.0	39.0
85-89	35.163384663530415	36.0	34.0	38.0	32.0	39.0
90-94	35.10542823162715	36.0	34.0	37.4	32.0	39.0
95-99	35.038996708750915	35.8	34.0	37.0	32.0	39.0
100-104	34.98502157923801	35.4	34.0	37.0	31.6	39.0
105-109	34.918407533673545	35.0	34.0	37.0	31.0	39.0
110-114	34.85342453909485	35.0	34.0	37.0	31.0	39.0
115-119	34.78006209245667	35.0	34.0	37.0	31.0	39.0
120-124	34.70065471469061	35.0	34.0	37.0	30.6	39.0
125-129	34.635757571404305	35.0	34.0	37.0	30.0	39.0
130-134	34.54357460951337	35.0	34.0	37.0	30.0	39.0
135-139	34.448303907604526	35.0	34.0	37.0	30.0	39.0
140-144	34.35045808490699	35.0	34.0	37.0	30.0	39.0
145-149	34.31576445045186	35.0	34.0	37.0	29.4	39.0
150	35.045901215703324	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	124.0
22	1276.0
23	7225.0
24	26679.0
25	67356.0
26	135648.0
27	234910.0
28	343450.0
29	446901.0
30	568536.0
31	743104.0
32	1006116.0
33	1442639.0
34	2260810.0
35	4088544.0
36	1.1006966E7
37	1696274.0
38	8.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.38398699848189	18.776850011004473	16.724261380554452	31.11490160995919
2	24.789504917892796	24.9424087448994	32.94554557019163	17.322540767016168
3	20.004919304753212	29.182811856155777	30.585926826047878	20.226342013043137
4	23.812556198305813	33.54521704938797	22.534978091356948	20.10724866094927
5	25.494643734774357	35.10391076868878	22.341549384254094	17.059896112282765
6	18.952751290642567	39.17729487498075	22.5971569957236	19.272796838653075
7	20.383972419995157	20.818232727722506	38.035674761520255	20.76212009076209
8	21.539560468177925	23.40709369227921	28.42191037736143	26.631435462181436
9	23.59852753112258	23.1959411161972	28.67999054682623	24.52554080585399
10-14	23.094048055010084	29.35101903188896	25.65576940829021	21.89916350481074
15-19	23.021711985700158	28.34521554783092	27.089858296525144	21.543214169943774
20-24	23.096338056023804	28.428072856183856	26.989456962823688	21.486132124968652
25-29	23.202822926668176	28.335354038965594	26.92736877461755	21.534454259748678
30-34	23.145343370011062	28.14449262599777	27.13509978575605	21.575064218235116
35-39	23.245739333417593	28.103389963347357	27.090244114060585	21.560626589174465
40-44	23.373579786458812	27.890495877465614	27.203437133681774	21.532487202393796
45-49	23.37308157081227	27.720638877639598	27.304192563486446	21.60208698806169
50-54	23.428893890228327	27.714463209887803	27.253223968042896	21.603418931840977
55-59	23.59248801729382	27.589615847737136	27.18657094595647	21.631325189012575
60-64	23.4908621080778	27.577411709955076	27.248541783604345	21.68318439836278
65-69	23.585082251099802	27.500622430966647	27.219039080889512	21.695256237044042
70-74	23.671805073953987	27.43961608523639	27.144063805878922	21.744515034930707
75-79	23.582519924134168	27.51598465029653	27.2168739513272	21.684621474242093
80-84	23.700674908007038	27.550642190500913	27.064075407397166	21.68460749409488
85-89	23.71336809373721	27.518502989149678	27.135189062912428	21.632939854200686
90-94	23.661262709589334	27.439626888458342	27.18092251185545	21.71818789009688
95-99	23.71246294751397	27.544423420431723	27.20558223881437	21.53753139323993
100-104	23.90327889653395	27.50002272046716	27.021945047106797	21.574753335892094
105-109	23.659000585618088	27.629792438085694	27.153920940835626	21.557286035460592
110-114	23.711658595083442	27.625276385437168	27.063066348704858	21.59999867077453
115-119	23.778953685202993	27.6230813050326	27.099361297368084	21.498603712396324
120-124	23.728342797347956	27.536226104479482	27.26993347045925	21.465497627713308
125-129	23.704056438157608	27.647596645300005	27.225719247008897	21.422627669533494
130-134	23.773336358891964	27.55045660482302	27.294332985216833	21.381874051068184
135-139	23.658615809000082	27.562925255399833	27.39265398924564	21.38580494635444
140-144	23.686681892897433	27.69737237400957	27.342871195577263	21.273074537515733
145-149	23.438828065505195	27.905255866460998	27.20701576174117	21.44890030629264
150	23.680975528379562	26.63518525733537	27.52959896430057	22.1542402499845
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	2.0
2	13.5
3	34.0
4	50.0
5	52.5
6	48.0
7	40.0
8	33.5
9	28.5
10	22.0
11	16.0
12	17.5
13	20.5
14	28.5
15	44.5
16	72.0
17	132.5
18	214.5
19	368.5
20	603.5
21	989.0
22	1594.5
23	2649.0
24	4399.0
25	7141.5
26	11045.0
27	17451.0
28	28444.0
29	43091.0
30	58746.5
31	77814.0
32	104677.5
33	140252.5
34	186896.0
35	256309.5
36	357889.5
37	481137.5
38	627387.5
39	813917.5
40	1032170.0
41	1234539.5
42	1413357.0
43	1577166.0
44	1692559.5
45	1743792.0
46	1737811.0
47	1678101.0
48	1571365.0
49	1423360.5
50	1238919.0
51	1039388.5
52	847882.0
53	674814.0
54	525339.5
55	397094.0
56	292361.5
57	216727.0
58	159147.0
59	113477.0
60	78183.5
61	52096.5
62	35368.0
63	24582.5
64	16600.5
65	10927.5
66	7405.5
67	5472.0
68	4644.0
69	3385.5
70	1827.5
71	1146.0
72	645.0
73	464.5
74	392.5
75	269.5
76	183.5
77	64.5
78	17.5
79	3.0
80	1.0
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.1534150229769E-6
3	0.0
4	8.3068300459538E-6
5	8.3068300459538E-6
6	0.0
7	0.0
8	0.0
9	1.2460245068930697E-5
10-14	0.10485379293805643
15-19	1.6613660091907598E-6
20-24	1.6613660091907598E-6
25-29	0.0
30-34	1.6613660091907598E-6
35-39	0.0
40-44	0.0
45-49	8.306830045953799E-7
50-54	2.4920490137861396E-6
55-59	0.0
60-64	1.6613661471976221E-6
65-69	1.6613664922148784E-6
70-74	0.0
75-79	3.3227414028738805E-6
80-84	0.0
85-89	0.0
90-94	8.307036440327226E-7
95-99	8.307149544101556E-6
100-104	1.6614600937887576E-6
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	8.313953922437827E-7
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
58-59	1.0
60-61	1.0
62-63	2.0
64-65	2.0
66-67	2.0
68-69	3.0
70-71	5.0
72-73	20.0
74-75	13.0
76-77	32.0
78-79	38.0
80-81	40.0
82-83	63.0
84-85	66.0
86-87	82.0
88-89	104.0
90-91	116.0
92-93	141.0
94-95	120.0
96-97	142.0
98-99	174.0
100-101	199.0
102-103	181.0
104-105	175.0
106-107	228.0
108-109	233.0
110-111	205.0
112-113	256.0
114-115	240.0
116-117	271.0
118-119	314.0
120-121	340.0
122-123	342.0
124-125	381.0
126-127	416.0
128-129	471.0
130-131	631.0
132-133	802.0
134-135	1129.0
136-137	1534.0
138-139	3413.0
140-141	7013.0
142-143	10223.0
144-145	66342.0
146-147	830178.0
148-149	922591.0
150-151	2.2227298E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.01309086403929
#Duplication Level	Percentage of total
1	24.81863245366605
2	11.32514031065264
3	7.5288255584593085
4	5.40940678670417
5	4.19273617213303
6	3.328860666830533
7	2.7541068043455645
8	2.2765661310305036
9	1.9844390619999037
>10	22.918763439107316
>50	5.653966126735633
>100	6.135864935032933
>500	0.8331350173976463
>1k	0.7518605753344874
>5k	0.08769596057048193
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.1534150229769E-6	4.1534150229769E-6	0.0	0.0	0.0017195138195124364	0.0
2	2.0767075114884496E-5	1.66136600919076E-5	0.0	0.0	0.005594650035949883	0.0
3	4.5687565252745894E-5	2.4920490137861398E-5	0.0	0.0	0.006882208693072722	0.0
4	9.55285455284687E-5	2.4920490137861398E-5	0.0	0.0	0.008173920765218539	0.0
5	9.968196055144559E-5	2.4920490137861398E-5	4.1534150229769E-6	0.0	0.00969822407865106	0.0
6	1.0383537557442248E-4	2.4920490137861398E-5	4.1534150229769E-6	0.0	0.011504959613646011	0.0
7	1.329092807352608E-4	3.73807352067921E-5	4.1534150229769E-6	0.0	0.013440451014353247	0.0
8	1.7444343096502979E-4	4.153415022976899E-5	4.1534150229769E-6	0.0	0.018557458322660786	0.0
9	1.8275026101098358E-4	4.153415022976899E-5	4.1534150229769E-6	0.0	0.024534222540724546	0.0
10-11	2.2636111875224102E-4	5.3994395298699696E-5	4.1534150229769E-6	0.0	0.03060859201182826	0.0
12-13	2.6997197649349845E-4	6.437793285614195E-5	4.1534150229769E-6	0.0	0.03615963119003689	0.0
14-15	3.1565954174624436E-4	6.64546403676304E-5	4.1534150229769E-6	4.1534150229769E-6	0.044626367714375295	0.0
16-17	3.447334469070827E-4	6.64546403676304E-5	4.1534150229769E-6	8.3068300459538E-6	0.05038923105875574	0.0
18-19	3.9042101215982854E-4	6.853134787911883E-5	4.1534150229769E-6	8.3068300459538E-6	0.05819142117941785	0.0
20-21	4.2780174736662064E-4	7.268476290209574E-5	4.1534150229769E-6	1.038353755744225E-5	0.06408719380453357	0.0
22-23	4.8179614266532036E-4	7.47614704135842E-5	4.1534150229769E-6	1.2460245068930699E-5	0.06993520215688503	0.0
24-25	5.295604154295548E-4	8.929842299400335E-5	4.1534150229769E-6	1.2460245068930699E-5	0.07507089983279597	2.07670751148845E-6
26-27	5.710945656593237E-4	9.55285455284687E-5	4.1534150229769E-6	1.2460245068930699E-5	0.07888996494642322	4.1534150229769E-6
28-29	6.936203088371423E-4	9.968196055144559E-5	4.1534150229769E-6	1.2460245068930699E-5	0.08276717787037216	4.1534150229769E-6
30-31	8.576802022447297E-4	1.0383537557442248E-4	8.3068300459538E-6	1.2460245068930699E-5	0.08756437222191049	4.1534150229769E-6
32-33	0.0010362770482327363	1.1421891313186474E-4	8.3068300459538E-6	1.2460245068930699E-5	0.09232833925326499	4.1534150229769E-6
34-35	0.0011899534040828818	1.2667915820079544E-4	8.3068300459538E-6	1.2460245068930699E-5	0.09536863905008408	8.3068300459538E-6
36-37	0.0012564080444505122	1.4121611078121458E-4	8.3068300459538E-6	1.2460245068930699E-5	0.09866645057832774	8.3068300459538E-6
38-39	0.0012854819496113503	1.4536952580419148E-4	8.3068300459538E-6	1.66136600919076E-5	0.10209301797228368	8.3068300459538E-6
40-41	0.0013124791472607002	1.5575306336163374E-4	8.3068300459538E-6	1.66136600919076E-5	0.10474082004943146	1.038353755744225E-5
42-43	0.0013394763449100502	1.6821330843056444E-4	8.3068300459538E-6	1.66136600919076E-5	0.1074820739645962	1.2460245068930699E-5
44-45	0.0013498598824674924	1.8067355349949513E-4	8.3068300459538E-6	1.66136600919076E-5	0.10970207429437737	1.2460245068930699E-5
46-47	0.0013768570801168422	2.2428441124075257E-4	8.3068300459538E-6	1.8690367603396046E-5	0.112106901592681	1.2460245068930699E-5
48-49	0.0014121611078121458	2.2843782626372948E-4	8.3068300459538E-6	2.0767075114884496E-5	0.11464256146420838	1.2460245068930699E-5
50-51	0.0014723856256453108	2.3259124128670636E-4	8.3068300459538E-6	2.0767075114884496E-5	0.11682518105878274	1.2460245068930699E-5
52-53	0.001505612945829126	2.3674465630968327E-4	8.3068300459538E-6	2.0767075114884496E-5	0.11905971834114432	1.2460245068930699E-5
54-55	0.0015346868509899644	2.637418539590331E-4	8.3068300459538E-6	2.0767075114884496E-5	0.12099313303434006	1.2460245068930699E-5
56-57	0.0015575306336163375	2.741253915164754E-4	8.3068300459538E-6	2.0767075114884496E-5	0.12310514457352381	1.2460245068930699E-5
58-59	0.0016198318589609908	2.845089290739176E-4	8.3068300459538E-6	2.0767075114884496E-5	0.12513408781224802	1.2460245068930699E-5
60-61	0.0017049768669320173	3.0527600418880213E-4	8.3068300459538E-6	2.0767075114884496E-5	0.12658155294775547	1.66136600919076E-5
62-63	0.0017465110171617864	3.1565954174624436E-4	8.3068300459538E-6	2.0767075114884496E-5	0.12851912105597418	1.8690367603396046E-5
64-65	0.0017672780922766708	3.2811978681517506E-4	8.3068300459538E-6	2.2843782626372947E-5	0.13030716622336574	2.4920490137861398E-5
66-67	0.0018171190725523936	3.4681015441857116E-4	8.3068300459538E-6	2.4920490137861398E-5	0.13170894379362047	2.4920490137861398E-5
68-69	0.0018441162702017434	3.571936919760134E-4	8.3068300459538E-6	2.4920490137861398E-5	0.13302765306341563	2.4920490137861398E-5
70-71	0.0018544998077591856	3.6550052202196715E-4	8.3068300459538E-6	2.4920490137861398E-5	0.13444812100127373	2.9073905160838295E-5
72-73	0.0018960339579889548	3.758840595794094E-4	8.3068300459538E-6	2.4920490137861398E-5	0.13543248036171926	2.9073905160838295E-5
74-75	0.0019043407880349086	3.9042101215982854E-4	1.038353755744225E-5	2.4920490137861398E-5	0.13654144217285408	2.9073905160838295E-5
76-77	0.0019562584758221198	4.070346722517362E-4	2.0767075114884496E-5	2.4920490137861398E-5	0.13789960888536754	2.9073905160838295E-5
78-79	0.002008176163609331	4.3818528492406287E-4	2.9073905160838295E-5	2.4920490137861398E-5	0.13940937524621963	2.9073905160838295E-5
80-81	0.002012329578632308	4.4856882248150515E-4	2.9073905160838295E-5	2.4920490137861398E-5	0.14069485719583097	2.9073905160838295E-5
82-83	0.0020310199462357037	4.672591900849012E-4	3.32273201838152E-5	2.4920490137861398E-5	0.14211532513368907	2.9073905160838295E-5
84-85	0.0020476336063276115	4.6933589759638966E-4	3.32273201838152E-5	2.4920490137861398E-5	0.1436977762574433	3.32273201838152E-5
86-87	0.002072554096465473	4.7348931261936654E-4	3.32273201838152E-5	2.4920490137861398E-5	0.14549205154736933	3.32273201838152E-5
88-89	0.0020850143415344037	4.8387285017680883E-4	3.32273201838152E-5	2.6997197649349848E-5	0.14718664487674388	3.530402769530365E-5
90-91	0.0020974745866033343	4.98409802757228E-4	3.32273201838152E-5	3.115061267232674E-5	0.1480983194742873	3.73807352067921E-5
92-93	0.0021078581241607765	5.025632177802048E-4	3.32273201838152E-5	3.73807352067921E-5	0.14899338041173882	3.73807352067921E-5
94-95	0.0021161649542067304	5.108700478261587E-4	3.32273201838152E-5	3.73807352067921E-5	0.1500421177050405	3.73807352067921E-5
96-97	0.0021182416617182187	5.274837079180662E-4	3.32273201838152E-5	3.73807352067921E-5	0.1509911730377907	4.1534150229769E-5
98-99	0.0021182416617182187	5.274837079180662E-4	3.530402769530365E-5	3.73807352067921E-5	0.15194853520058688	5.3994395298699696E-5
100-101	0.0021265484917641726	5.295604154295547E-4	3.73807352067921E-5	3.73807352067921E-5	0.15282698247794652	5.814781032167659E-5
102-103	0.002128625199275661	5.420206604984854E-4	4.153415022976899E-5	3.73807352067921E-5	0.15378642134825415	6.23012253446535E-5
104-105	0.0021390087368331036	5.461740755214623E-4	4.153415022976899E-5	3.73807352067921E-5	0.15468355899321717	6.64546403676304E-5
106-107	0.002155622396925011	5.731712731708121E-4	4.153415022976899E-5	3.73807352067921E-5	0.15552254882785854	7.060805539060729E-5
108-109	0.002159775811947988	6.063985933546273E-4	4.5687565252745894E-5	3.73807352067921E-5	0.15632831134231603	7.060805539060729E-5
110-111	0.0021722360570169187	6.250889609580234E-4	4.9840980275722795E-5	3.73807352067921E-5	0.15731890082529604	7.268476290209574E-5
112-113	0.0021763894720398954	6.458560360729079E-4	5.191768778721124E-5	3.73807352067921E-5	0.158135046877311	7.891488543656109E-5
114-115	0.0021826195945743605	6.500094510958848E-4	5.3994395298699696E-5	3.73807352067921E-5	0.15892834914669957	7.891488543656109E-5
116-117	0.0021909264246203148	6.541628661188617E-4	5.3994395298699696E-5	3.73807352067921E-5	0.15996462619493232	8.099159294804953E-5
118-119	0.00219715654715478	6.624696961648154E-4	5.814781032167659E-5	3.73807352067921E-5	0.16079946261455066	8.722171548251489E-5
120-121	0.00219715654715478	6.645464036763039E-4	5.814781032167659E-5	3.9457442718280545E-5	0.16162599220412305	8.722171548251489E-5
122-123	0.00219715654715478	6.686998186992808E-4	5.814781032167659E-5	4.153415022976899E-5	0.16240268081341974	9.137513050549179E-5
124-125	0.002201309962177757	6.707765262107693E-4	5.814781032167659E-5	4.153415022976899E-5	0.16329358833584828	9.55285455284687E-5
126-127	0.0022096167922237104	6.770066487452346E-4	5.814781032167659E-5	4.153415022976899E-5	0.16429248464887425	9.968196055144559E-5
128-129	0.0022220770372926415	6.811600637682115E-4	5.814781032167659E-5	4.153415022976899E-5	0.16520831266144065	1.0798879059739939E-4
130-131	0.002234537282361572	6.915436013256537E-4	6.23012253446535E-5	4.153415022976899E-5	0.1662778170298572	1.0798879059739939E-4
132-133	0.0022553043574764565	6.977737238601191E-4	6.64546403676304E-5	4.153415022976899E-5	0.16710434661942958	1.2044903566633009E-4
134-135	0.002269841310056876	7.102339689290498E-4	7.060805539060729E-5	4.153415022976899E-5	0.1679682569442088	1.2044903566633009E-4
136-137	0.0022739947250798526	7.185407989750036E-4	7.268476290209574E-5	4.153415022976899E-5	0.16891731227695903	1.287558657122839E-4
138-139	0.002276071432591341	7.185407989750036E-4	7.47614704135842E-5	4.153415022976899E-5	0.16975214869657737	1.329092807352608E-4
>>END_MODULE
