##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.4d.S.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24210574
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	63-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.12604191044789	37.0	35.0	38.0	33.0	39.0
2	35.52378018794598	35.0	34.0	38.0	33.0	39.0
3	35.5923980984507	35.0	35.0	38.0	33.0	39.0
4	35.683479292973395	36.0	35.0	38.0	33.0	39.0
5	35.607097915150625	36.0	35.0	38.0	33.0	39.0
6	35.5451992588032	35.0	35.0	37.0	33.0	39.0
7	35.5627372155654	35.0	34.0	38.0	33.0	39.0
8	35.69110203665555	36.0	35.0	38.0	33.0	39.0
9	35.678858791204206	36.0	35.0	38.0	33.0	39.0
10-14	35.64898091222455	35.8	35.0	37.8	33.0	39.0
15-19	35.62915305518985	36.0	35.0	38.0	33.0	39.0
20-24	35.61943701954361	36.0	35.0	38.0	33.0	39.0
25-29	35.604443959073414	36.0	35.0	38.0	33.0	39.0
30-34	35.588748007378925	36.0	35.0	38.0	33.0	39.0
35-39	35.5636254885985	36.0	35.0	38.0	33.0	39.0
40-44	35.54966417566143	36.0	35.0	38.0	33.0	39.0
45-49	35.521704342904044	36.0	35.0	38.0	33.0	39.0
50-54	35.50443685473958	36.0	35.0	38.0	33.0	39.0
55-59	35.479313336395904	36.0	35.0	38.0	33.0	39.0
60-64	35.44023556349309	36.0	34.2	38.0	33.0	39.0
65-69	35.417504828598865	36.0	34.0	38.0	33.0	39.0
70-74	35.38755102510022	36.0	34.0	38.0	32.8	39.0
75-79	35.340726886486365	36.0	34.0	38.0	32.0	39.0
80-84	35.30385895645731	36.0	34.0	38.0	32.0	39.0
85-89	35.25648823831991	36.0	34.0	38.0	32.0	39.0
90-94	35.207410298375095	36.0	34.0	38.0	32.0	39.0
95-99	35.14475781527133	36.0	34.0	38.0	32.0	39.0
100-104	35.10051262718732	36.0	34.0	38.0	32.0	39.0
105-109	35.04511988960653	36.0	34.0	38.0	32.0	39.0
110-114	34.973852929824766	35.4	34.0	38.0	31.0	39.0
115-119	34.90764150684108	35.0	34.0	37.6	31.0	39.0
120-124	34.836034885214396	35.0	34.0	37.0	31.0	39.0
125-129	34.762881621554826	35.0	34.0	37.0	31.0	39.0
130-134	34.66417410196313	35.0	34.0	37.0	30.2	39.0
135-139	34.61189260649987	35.0	34.0	37.0	30.0	39.0
140-144	34.49867291307084	35.0	34.0	37.0	30.0	39.0
145-149	34.48605912677844	35.0	34.0	37.0	30.0	39.0
150	35.204122421681696	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	129.0
22	1155.0
23	6651.0
24	23891.0
25	60087.0
26	120179.0
27	210264.0
28	317998.0
29	427710.0
30	560994.0
31	744293.0
32	1013540.0
33	1436172.0
34	2189799.0
35	3805584.0
36	1.0877296E7
37	2414820.0
38	8.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.79395193191206	19.269315960868997	16.543742416020372	31.392989691198565
2	24.823397413047704	25.296496481248237	31.942414913417583	17.93769119228648
3	20.362693589999147	29.165326687421782	30.159103208374987	20.312876514204085
4	23.707426350155927	33.72633379117736	21.945365690214533	20.62087416845218
5	25.864500362527547	34.89905691620529	21.81317964621574	17.42326307505142
6	19.337633217618052	39.25466203320912	21.893231445070242	19.514473304102577
7	20.700541837628467	20.990088876042346	37.18262937508214	21.126739911247043
8	21.65417061156832	23.559065555405667	28.250734575727115	26.5360292572989
9	23.84101260878821	23.268465258196688	27.965586441692793	24.92493569132231
10-14	23.145253805217507	29.019026149483278	25.394025767418814	22.4416942778804
15-19	23.259251928516854	27.805604278527223	26.818942830516946	22.116200962438974
20-24	23.104097408016845	27.920528443480936	26.736626731774304	22.238747416727914
25-29	23.26331379008197	27.497275364062002	26.719642417399935	22.519768428456093
30-34	23.08421683847727	27.244695644142926	26.950952918340555	22.720134599039245
35-39	23.392938143474005	26.975040740463236	26.934208994797064	22.69781212126569
40-44	23.52830957250332	26.66070866390859	27.041200262331657	22.769781501256432
45-49	23.439832723006344	26.496357554317857	27.14837266682213	22.915437055853673
50-54	23.60878432704652	26.484099055230992	26.916800898648663	22.990315719073823
55-59	23.699334018268218	26.455780024050647	26.951958264186548	22.892927693494585
60-64	23.44287271704399	26.41982570442011	27.02921233531442	23.10808924322149
65-69	23.60224125914197	26.37991653347897	26.959630284449236	23.058211922929825
70-74	23.79393455027372	26.31567338690934	26.75683369658936	23.133558366227582
75-79	23.64331842710124	26.43186864592985	26.961970719469313	22.9628422074996
80-84	23.93429455380398	26.435266611446544	26.63577219046428	22.994666644285193
85-89	23.745443256258003	26.52390051605598	26.82619495558514	22.904461272100875
90-94	23.75186074487192	26.322897009166923	26.810575587577457	23.1146666583837
95-99	23.740135541881283	26.534974127185635	26.85159357380928	22.873296757123804
100-104	23.975062628599616	26.281469111705526	26.82633016298834	22.917138096706516
105-109	23.77472649823272	26.4800714850804	26.791140466340767	22.954061550346104
110-114	23.862865254892164	26.582122281422045	26.689506430772802	22.865506032912986
115-119	23.968374110278557	26.592216170311804	26.836599517450782	22.60281020195886
120-124	23.867496006007155	26.678521452801117	27.021700112712228	22.432282428479503
125-129	23.9055859046155	26.767223154055582	26.833235846565856	22.493955094763063
130-134	24.07347133214381	26.75305804992804	26.804722956075146	22.368747661853007
135-139	23.857928779038723	26.821906332455626	27.00633500304116	22.313829885464497
140-144	23.882759600265917	26.929315947315622	26.857865947926157	22.330058504492303
145-149	23.62803035794257	27.217669355677504	26.68897093734186	22.46532934903806
150	23.93034482949751	26.020740173351207	26.911392737765638	23.13752225938564
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.5
6	1.0
7	1.0
8	0.5
9	2.0
10	4.5
11	8.0
12	14.5
13	15.0
14	25.5
15	51.5
16	71.0
17	121.5
18	225.0
19	386.0
20	638.0
21	1040.0
22	1546.0
23	2345.5
24	3901.5
25	6091.5
26	9075.5
27	13901.5
28	20355.0
29	28305.5
30	39842.0
31	56864.0
32	83052.5
33	112789.5
34	147593.5
35	205447.5
36	291070.5
37	389659.5
38	506138.5
39	659850.5
40	844212.5
41	1034976.0
42	1224860.0
43	1407139.0
44	1546595.5
45	1627849.5
46	1648299.0
47	1607328.0
48	1544927.5
49	1477617.5
50	1359576.5
51	1194771.5
52	1016474.0
53	843124.0
54	694659.5
55	577827.5
56	473109.0
57	386643.5
58	323594.5
59	263697.0
60	198869.0
61	134160.0
62	83149.5
63	50906.0
64	31253.5
65	15424.5
66	8051.5
67	4233.5
68	2298.5
69	1380.5
70	919.0
71	789.5
72	497.0
73	344.0
74	290.0
75	190.5
76	121.0
77	37.5
78	9.0
79	3.0
80	2.0
81	0.0
82	0.5
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	8.260853294928075E-7
50-54	0.0
55-59	0.0
60-64	0.0
65-69	1.652170945600793E-6
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	8.260980977116926E-7
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	2.4785120850844777E-6
130-134	0.05245905085024824
135-139	0.0
140-144	0.0
145-149	8.420561296259185E-7
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62-63	1.0
64-65	3.0
66-67	0.0
68-69	1.0
70-71	5.0
72-73	4.0
74-75	8.0
76-77	11.0
78-79	22.0
80-81	20.0
82-83	24.0
84-85	23.0
86-87	30.0
88-89	40.0
90-91	50.0
92-93	52.0
94-95	58.0
96-97	44.0
98-99	71.0
100-101	83.0
102-103	82.0
104-105	90.0
106-107	87.0
108-109	110.0
110-111	121.0
112-113	130.0
114-115	155.0
116-117	141.0
118-119	158.0
120-121	220.0
122-123	222.0
124-125	271.0
126-127	325.0
128-129	355.0
130-131	447.0
132-133	602.0
134-135	807.0
136-137	1347.0
138-139	3916.0
140-141	8337.0
142-143	8787.0
144-145	60445.0
146-147	797431.0
148-149	953976.0
150-151	2.2371462E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.47627129521732
#Duplication Level	Percentage of total
1	25.455565267335427
2	8.815642897349584
3	5.555105253798284
4	3.9847636911377946
5	3.000710121382806
6	2.423810528917459
7	1.923007178738033
8	1.6998293859937532
9	1.4371652774570152
>10	17.797485715229282
>50	6.279580912381011
>100	12.639971479856154
>500	3.759578953092625
>1k	4.407167970100505
>5k	0.5668796887880199
>10k+	0.25373567844249034
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.0978199855980285E-4	8.260853294928076E-6	0.0	4.130426647464038E-6	0.0018834745512436013	0.0
2	0.0016108663925109748	2.065213323732019E-5	0.0	4.130426647464038E-6	0.009549546408936856	0.0
3	0.0024039083088240702	2.065213323732019E-5	0.0	4.130426647464038E-6	0.013163669725467888	0.0
4	0.005894118825931182	2.065213323732019E-5	0.0	4.130426647464038E-6	0.01871083271301209	0.0
5	0.006092379305009455	2.065213323732019E-5	0.0	4.130426647464038E-6	0.022395173282550012	0.0
6	0.007129116393522929	2.065213323732019E-5	0.0	4.130426647464038E-6	0.029371463890116772	0.0
7	0.009289329530146622	2.4782559884784227E-5	0.0	4.130426647464038E-6	0.03882601048616195	0.0
8	0.01577409936666516	2.4782559884784227E-5	0.0	4.130426647464038E-6	0.05418293676143325	0.0
9	0.016393663363784765	2.4782559884784227E-5	0.0	4.130426647464038E-6	0.06587617460040394	0.0
10-11	0.01701322736090437	4.130426647464038E-5	0.0	4.130426647464038E-6	0.11213488783867742	0.0
12-13	0.017674095624498617	4.130426647464038E-5	0.0	4.130426647464038E-6	0.13431114850891185	0.0
14-15	0.02033615559878919	4.130426647464038E-5	0.0	4.130426647464038E-6	0.1442737375825951	0.0
16-17	0.02335136705143794	4.130426647464038E-5	0.0	4.130426647464038E-6	0.1508307898854443	0.0
18-19	0.029648202475496863	4.7499906445836434E-5	0.0	4.130426647464038E-6	0.15785458039945688	0.0
20-21	0.03531101740917006	4.956511976956845E-5	0.0	4.130426647464038E-6	0.16308576574847006	0.0
22-23	0.03748362182573614	5.369554641703249E-5	0.0	4.130426647464038E-6	0.16823227735121027	0.0
24-25	0.04224393853693845	6.815203968315662E-5	0.0	4.130426647464038E-6	0.17323628923461293	0.0
26-27	0.04645077807738057	7.021725300688864E-5	0.0	4.130426647464038E-6	0.1772923682024226	0.0
28-29	0.057070104988010614	7.021725300688864E-5	2.065213323732019E-6	4.130426647464038E-6	0.18084040469259424	0.0
30-31	0.07909147465896513	7.434767965435268E-5	4.130426647464038E-6	4.130426647464038E-6	0.1846631145548222	0.0
32-33	0.09765567722599225	9.499981289167287E-5	4.130426647464038E-6	4.130426647464038E-6	0.18794267331290865	0.0
34-35	0.11206673579899427	9.499981289167287E-5	4.130426647464038E-6	6.195639971196057E-6	0.19103016723188804	0.0
36-37	0.11462760032042198	9.91302395391369E-5	4.130426647464038E-6	8.260853294928076E-6	0.19307679363570646	0.0
38-39	0.11665977023097429	1.0119545286286892E-4	4.130426647464038E-6	8.260853294928076E-6	0.19485907273408717	0.0
40-41	0.11807237614440698	1.0532587951033297E-4	4.130426647464038E-6	8.260853294928076E-6	0.19650091732645414	0.0
42-43	0.11835117994311081	1.1152151948152902E-4	4.130426647464038E-6	8.260853294928076E-6	0.19798993613286492	2.065213323732019E-6
44-45	0.11902443948664744	1.197823727764571E-4	4.130426647464038E-6	8.260853294928076E-6	0.19926210754028384	4.130426647464038E-6
46-47	0.12044530625337507	1.2391279942392113E-4	4.130426647464038E-6	8.260853294928076E-6	0.20066232217377417	4.130426647464038E-6
48-49	0.12155845623486664	1.321736527188492E-4	4.130426647464038E-6	8.260853294928076E-6	0.20191797187460322	6.195639971196057E-6
50-51	0.12247128052395617	1.3423886604258125E-4	4.130426647464038E-6	8.260853294928076E-6	0.20334709949462576	8.260853294928076E-6
52-53	0.12337377874642708	1.3836929269004527E-4	4.130426647464038E-6	8.260853294928076E-6	0.20449329288929705	1.4456493266124132E-5
54-55	0.12387356037077023	1.4043450601377728E-4	4.130426647464038E-6	8.260853294928076E-6	0.20557546467093263	1.652170658985615E-5
56-57	0.12470171091358677	1.4043450601377728E-4	4.130426647464038E-6	8.260853294928076E-6	0.206798070958582	1.652170658985615E-5
58-59	0.1248524714862192	1.4663014598497335E-4	6.195639971196057E-6	8.260853294928076E-6	0.20769850396772915	1.652170658985615E-5
60-61	0.12514573177818916	1.507605726324374E-4	8.260853294928076E-6	8.260853294928076E-6	0.2085658935636966	2.065213323732019E-5
62-63	0.12539562259036072	1.528257859561694E-4	8.260853294928076E-6	8.260853294928076E-6	0.20961502193215245	2.065213323732019E-5
64-65	0.12541833993692175	1.6108663925109749E-4	8.260853294928076E-6	8.260853294928076E-6	0.2107323023402915	2.4782559884784227E-5
66-67	0.12546790505669134	1.858691991358817E-4	8.260853294928076E-6	8.260853294928076E-6	0.21160175714958265	2.4782559884784227E-5
68-69	0.12550920932316598	1.858691991358817E-4	8.260853294928076E-6	1.0326066618660094E-5	0.21237414693265844	2.4782559884784227E-5
70-71	0.12558975264279154	1.858691991358817E-4	8.260853294928076E-6	1.652170658985615E-5	0.2130680586094324	2.4782559884784227E-5
72-73	0.12564757861585601	1.8793441245961373E-4	8.260853294928076E-6	1.652170658985615E-5	0.21367729653993334	2.4782559884784227E-5
74-75	0.12568475245568322	1.8999962578334574E-4	8.260853294928076E-6	1.652170658985615E-5	0.2144868601628363	2.8912986532248264E-5
76-77	0.12571366544221546	1.941300524308098E-4	1.2391279942392113E-5	1.652170658985615E-5	0.21527577165250192	2.8912986532248264E-5
78-79	0.12576323056198502	2.065213323732019E-4	1.4456493266124132E-5	1.652170658985615E-5	0.21627946532783568	3.30434131797123E-5
80-81	0.12579007833519354	2.065213323732019E-4	2.065213323732019E-5	1.652170658985615E-5	0.21721294175016256	3.30434131797123E-5
82-83	0.12582931738834446	2.065213323732019E-4	2.4782559884784227E-5	1.652170658985615E-5	0.21805554878624522	3.717383982717634E-5
84-85	0.1258582303748767	2.085865456969339E-4	2.4782559884784227E-5	1.858691991358817E-5	0.2189250035955364	3.717383982717634E-5
86-87	0.12587681729479028	2.16847398991862E-4	2.4782559884784227E-5	2.065213323732019E-5	0.21974489328505803	3.923905315090836E-5
88-89	0.1259036650679988	2.1891261231559402E-4	2.4782559884784227E-5	2.065213323732019E-5	0.22060815245437798	4.130426647464038E-5
90-91	0.12594083890782598	2.2304303896305804E-4	2.4782559884784227E-5	2.065213323732019E-5	0.22125869465135356	4.130426647464038E-5
92-93	0.12594703454779715	2.2304303896305804E-4	2.4782559884784227E-5	2.065213323732019E-5	0.22188858471509185	4.130426647464038E-5
94-95	0.12597181710768196	2.2304303896305804E-4	2.4782559884784227E-5	2.065213323732019E-5	0.222619670231693	4.3369479798372397E-5
96-97	0.12598420838762436	2.2304303896305804E-4	2.4782559884784227E-5	2.065213323732019E-5	0.22334869053497036	4.5434693122104415E-5
98-99	0.12599659966756674	2.271734656105221E-4	2.4782559884784227E-5	2.2717346561052208E-5	0.22399716751862225	5.163033309330047E-5
100-101	0.12602138222745152	2.292386789342541E-4	2.6847773208516245E-5	2.4782559884784227E-5	0.22453412298279257	5.369554641703249E-5
102-103	0.12603583872071766	2.3130389225798611E-4	3.30434131797123E-5	2.4782559884784227E-5	0.2251867303930919	5.369554641703249E-5
104-105	0.12605029521398378	2.3130389225798611E-4	3.717383982717634E-5	2.6847773208516245E-5	0.2259611853894914	5.782597306449653E-5
106-107	0.1260709473472211	2.3749953222918218E-4	3.717383982717634E-5	2.8912986532248264E-5	0.22660966237314323	6.195639971196057E-5
108-109	0.12609572990710588	2.395647455529142E-4	3.717383982717634E-5	3.30434131797123E-5	0.2271941177437594	6.195639971196057E-5
110-111	0.12612670810696186	2.4369517220037824E-4	4.130426647464038E-5	3.717383982717634E-5	0.2278900946338571	6.195639971196057E-5
112-113	0.12614322981355172	2.4782559884784227E-4	4.3369479798372397E-5	3.717383982717634E-5	0.2284642239378546	6.195639971196057E-5
114-115	0.12614736024019918	2.4782559884784227E-4	4.5434693122104415E-5	3.717383982717634E-5	0.22908585314829794	6.195639971196057E-5
116-117	0.12615768630681784	2.4782559884784227E-4	4.5434693122104415E-5	3.717383982717634E-5	0.22968476501218021	6.195639971196057E-5
118-119	0.12616594716011276	2.4782559884784227E-4	4.5434693122104415E-5	3.717383982717634E-5	0.2303559593423931	6.195639971196057E-5
120-121	0.12617007758676024	2.4782559884784227E-4	4.5434693122104415E-5	3.717383982717634E-5	0.23098997983277886	6.402161303569259E-5
122-123	0.1261804036533789	2.4989081217157425E-4	4.956511976956845E-5	3.717383982717634E-5	0.23152900051027292	6.60868263594246E-5
124-125	0.12619279493332128	2.5402123881903833E-4	4.956511976956845E-5	3.717383982717634E-5	0.23213823844077386	7.021725300688864E-5
126-127	0.12620105578661622	2.5608645214277037E-4	4.956511976956845E-5	4.130426647464038E-5	0.23269791125150524	7.228246633062067E-5
128-129	0.12621757749320606	2.5608645214277037E-4	4.956511976956845E-5	4.130426647464038E-5	0.23342280112813518	7.434767965435268E-5
130-131	0.126225838346501	2.5608645214277037E-4	4.956511976956845E-5	4.130426647464038E-5	0.23417040835132616	7.434767965435268E-5
132-133	0.126225838346501	2.5608645214277037E-4	4.956511976956845E-5	4.130426647464038E-5	0.23487258088139504	7.847810630181672E-5
134-135	0.12622790355982472	2.6641251876143046E-4	4.956511976956845E-5	4.130426647464038E-5	0.23549834051848584	7.847810630181672E-5
136-137	0.12623409919979592	2.746733720563585E-4	4.956511976956845E-5	4.130426647464038E-5	0.23612410015557667	7.847810630181672E-5
138-139	0.12623409919979592	2.788037987038226E-4	4.956511976956845E-5	4.130426647464038E-5	0.23673746851272506	7.847810630181672E-5
>>END_MODULE
