##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.4d.S.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20058847
Total Bases	3 Gbp
Sequences flagged as poor quality	0
Sequence length	67-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.196988889740275	37.0	35.0	38.0	33.0	39.0
2	35.51932650964435	35.0	34.0	38.0	33.0	39.0
3	35.62141841951335	36.0	35.0	38.0	33.0	39.0
4	35.72639065445786	36.0	35.0	38.0	33.0	39.0
5	35.660537916262086	36.0	35.0	38.0	33.0	39.0
6	35.58288355257907	36.0	35.0	37.0	33.0	39.0
7	35.55676734560067	35.0	34.0	38.0	33.0	39.0
8	35.70171685341635	36.0	35.0	38.0	33.0	39.0
9	35.69882580987831	36.0	35.0	38.0	33.0	39.0
10-14	35.61628654927175	36.0	35.0	37.8	33.0	39.0
15-19	35.624754254319804	36.0	35.0	38.0	33.0	39.0
20-24	35.60928979616825	36.0	35.0	38.0	33.0	39.0
25-29	35.59751447328951	36.0	35.0	38.0	33.0	39.0
30-34	35.574054221561184	36.0	35.0	38.0	33.0	39.0
35-39	35.55158549242636	36.0	35.0	38.0	33.0	39.0
40-44	35.536611052469766	36.0	35.0	38.0	33.0	39.0
45-49	35.50472416485354	36.0	34.4	38.0	33.0	39.0
50-54	35.489371667274796	36.0	34.4	38.0	33.0	39.0
55-59	35.45772874183646	36.0	34.0	38.0	33.0	39.0
60-64	35.421752765749694	36.0	34.0	38.0	33.0	39.0
65-69	35.38927664295208	36.0	34.0	38.0	32.4	39.0
70-74	35.36128016889952	36.0	34.0	38.0	32.0	39.0
75-79	35.31415102102712	36.0	34.0	38.0	32.0	39.0
80-84	35.27603162856512	36.0	34.0	38.0	32.0	39.0
85-89	35.235168391062146	36.0	34.0	38.0	32.0	39.0
90-94	35.18236874136729	36.0	34.0	38.0	32.0	39.0
95-99	35.1200205166424	36.0	34.0	38.0	32.0	39.0
100-104	35.071250731538626	36.0	34.0	38.0	32.0	39.0
105-109	35.01039448696603	36.0	34.0	38.0	31.4	39.0
110-114	34.94235190331485	35.8	34.0	37.6	31.0	39.0
115-119	34.87278876444209	35.0	34.0	37.0	31.0	39.0
120-124	34.79790517432318	35.0	34.0	37.0	31.0	39.0
125-129	34.739905155812366	35.0	34.0	37.0	30.8	39.0
130-134	34.64962252092995	35.0	34.0	37.0	30.0	39.0
135-139	34.5632423603899	35.0	34.0	37.0	30.0	39.0
140-144	34.474581985444004	35.0	34.0	37.0	30.0	39.0
145-149	34.4403858006737	35.0	34.0	37.0	30.0	39.0
150	35.149811634706936	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	118.0
22	1175.0
23	6797.0
24	24292.0
25	59724.0
26	115920.0
27	192804.0
28	279756.0
29	361233.0
30	460647.0
31	597926.0
32	805688.0
33	1148182.0
34	1784183.0
35	3203398.0
36	9143773.0
37	1873221.0
38	3.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.296774236325746	19.362673238397	15.924439724775805	31.416112800501445
2	24.767540228010116	25.832900565022506	32.07116042113488	17.328398785832505
3	20.180865829426786	29.505115623046528	29.995273407290064	20.318745140236626
4	23.709120654298857	34.05356918339171	21.91956107544771	20.317749086861728
5	25.760508567616075	35.14019524651641	21.77789182000341	17.321404365864097
6	19.310334169290407	39.469605553061506	21.912737248829632	19.30732302881846
7	20.66395441373076	21.248140533700667	36.91472396195056	21.17318109061802
8	21.63550078426741	23.933798388312148	28.13545065676008	26.295250170660356
9	23.773182166112647	23.647855913545573	27.920015937108246	24.658945983233533
10-14	23.182101246258018	29.386300343815414	25.21617810374633	22.21542030618024
15-19	23.26730344969479	28.122420994586577	26.718570613754615	21.89170494196401
20-24	23.185298736263356	28.149248059970745	26.66134000623266	22.004113197533236
25-29	23.342990751163313	27.74757990825694	26.620093368277846	22.289335972301895
30-34	23.182414223509458	27.45704177313881	26.842846949278787	22.51769705407295
35-39	23.467450780869417	27.180209582138094	26.85181183995084	22.500527797041652
40-44	23.621399810481627	26.820526890907807	26.966800504204457	22.591272794406105
45-49	23.53560301845864	26.642653987041232	27.08320672668773	22.7385362678124
50-54	23.681169893821412	26.634683769943695	26.87327177246388	22.810874563771016
55-59	23.78676102370191	26.57353136997356	26.910862822773414	22.728844783551118
60-64	23.52688367382233	26.51874158071	26.992693049605492	22.96168169586218
65-69	23.67911247378613	26.502904972640902	26.898015431236367	22.919967122336608
70-74	23.894208892249758	26.42561414360354	26.691921629696402	22.9882553344503
75-79	23.745706005783987	26.56804011001639	26.87939924711461	22.806854637085014
80-84	24.016499173584606	26.545297682982866	26.59396161443089	22.844241529001643
85-89	23.865863696003448	26.635285712688976	26.723807685590117	22.775042905717456
90-94	23.85229283987137	26.46214388576865	26.72168562389655	22.963877650463427
95-99	23.858603091154205	26.683929715453537	26.757773479497722	22.69969371389454
100-104	24.074617025892692	26.422915862161357	26.737423615888346	22.765043496057604
105-109	23.884063524708814	26.61063145741575	26.699781040153447	22.805523977721986
110-114	23.976413248930907	26.72061252316938	26.592582416147014	22.710391811752697
115-119	24.0734143450183	26.731730162478723	26.738758913770855	22.456096578732126
120-124	23.98049724596052	26.804383863836662	26.952401320764714	22.262717569438106
125-129	24.01513618816996	26.89681169022619	26.76468744347595	22.3233646781279
130-134	24.17542757011883	26.891122740449582	26.704760654162307	22.228689035269287
135-139	23.981761940334714	26.94735896243099	26.910917549208254	22.159961548026047
140-144	23.987434014737257	27.059036258284586	26.791251507320123	22.162278219658035
145-149	23.75598031125436	27.305189481299042	26.625508293552674	22.313321913893922
150	24.217493451981657	25.997627098094377	26.807315190644115	22.97756425927985
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	10.0
3	24.5
4	34.0
5	32.0
6	28.0
7	29.0
8	24.5
9	19.0
10	13.5
11	13.5
12	17.0
13	17.0
14	21.5
15	32.0
16	56.0
17	107.5
18	199.0
19	328.0
20	566.0
21	867.0
22	1286.0
23	2061.0
24	3409.0
25	5331.5
26	8082.0
27	12383.0
28	17988.0
29	25004.0
30	35013.0
31	49697.5
32	71903.5
33	97507.5
34	127702.5
35	177329.0
36	249901.0
37	333458.5
38	433873.5
39	562274.5
40	713370.0
41	871760.0
42	1030515.0
43	1179603.5
44	1289740.0
45	1352021.5
46	1367684.0
47	1328014.0
48	1266733.0
49	1208843.0
50	1110774.5
51	971989.5
52	826336.5
53	686726.0
54	561695.0
55	465008.5
56	383318.0
57	315055.0
58	264316.0
59	214991.0
60	161676.5
61	108805.5
62	67223.5
63	40922.5
64	25229.5
65	12970.5
66	7089.5
67	3805.0
68	1979.5
69	1230.0
70	853.0
71	686.5
72	425.0
73	301.5
74	250.5
75	160.0
76	99.5
77	33.0
78	7.5
79	1.5
80	1.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	4.985331410125417E-6
5	0.0
6	9.970662820250835E-6
7	0.0
8	0.0
9	4.985331410125417E-6
10-14	0.09832170313677552
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	9.970662820250834E-7
40-44	9.970662820250834E-7
45-49	0.0
50-54	4.985331410125417E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	9.970664808533574E-7
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	5.982481201174541E-6
100-104	9.97088859482301E-7
105-109	9.970985230657605E-7
110-114	0.0
115-119	0.0
120-124	0.0
125-129	9.971716422972211E-7
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
66-67	1.0
68-69	1.0
70-71	1.0
72-73	4.0
74-75	4.0
76-77	8.0
78-79	14.0
80-81	15.0
82-83	14.0
84-85	23.0
86-87	25.0
88-89	41.0
90-91	37.0
92-93	28.0
94-95	32.0
96-97	55.0
98-99	81.0
100-101	68.0
102-103	73.0
104-105	77.0
106-107	87.0
108-109	106.0
110-111	107.0
112-113	127.0
114-115	131.0
116-117	133.0
118-119	161.0
120-121	164.0
122-123	174.0
124-125	218.0
126-127	215.0
128-129	299.0
130-131	395.0
132-133	499.0
134-135	651.0
136-137	1178.0
138-139	3512.0
140-141	6760.0
142-143	7395.0
144-145	49013.0
146-147	666766.0
148-149	729415.0
150-151	1.8590739E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.777066659627046
#Duplication Level	Percentage of total
1	23.90897799849427
2	9.734323466553757
3	6.158160963861098
4	4.365102517370745
5	3.1772750697280077
6	2.448494975459267
7	2.028199174660292
8	1.7256644461690525
9	1.4449211461205618
>10	18.43489028926502
>50	6.446671222810243
>100	12.351444478728103
>500	3.2662448733054523
>1k	3.8657291470939312
>5k	0.4498304295618685
>10k+	0.19406980081814337
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.5923723332652173E-4	4.985331410125417E-6	0.0	0.0	0.0034548346672169145	0.0
2	0.0013909074634249916	1.4955994230376252E-5	0.0	4.985331410125417E-6	0.013754529360536026	0.0
3	0.0021187658493033025	1.4955994230376252E-5	0.0	4.985331410125417E-6	0.01852549152002605	0.0
4	0.005349260603064573	1.4955994230376252E-5	0.0	4.985331410125417E-6	0.026367417828153335	0.0
5	0.005543688528059464	1.4955994230376252E-5	0.0	4.985331410125417E-6	0.03127796926712687	0.0
6	0.0066304907754668055	1.4955994230376252E-5	0.0	4.985331410125417E-6	0.04020171249125137	0.0
7	0.008938699218354874	1.4955994230376252E-5	0.0	4.985331410125417E-6	0.05192222663645622	0.0
8	0.01528502610344453	1.4955994230376252E-5	0.0	4.985331410125417E-6	0.07103598726287708	0.0
9	0.01576361791881657	1.4955994230376252E-5	0.0	4.985331410125417E-6	0.08501485653686874	0.0
10-11	0.016404233005017685	1.994132564050167E-5	0.0	4.985331410125417E-6	0.141827693286658	0.0
12-13	0.017147047385126374	2.4926657050627087E-5	0.0	4.985331410125417E-6	0.1697754611718211	0.0
14-15	0.01968458107288021	2.9911988460752504E-5	0.0	4.985331410125417E-6	0.18158321861670312	0.0
16-17	0.022663316590430145	2.9911988460752504E-5	0.0	4.985331410125417E-6	0.1897342354722582	0.0
18-19	0.02883764954187048	2.9911988460752504E-5	0.0	4.985331410125417E-6	0.198510911419784	0.0
20-21	0.03410215951096292	2.9911988460752504E-5	0.0	4.985331410125417E-6	0.20432131517828517	4.985331410125417E-6
22-23	0.03619101337180547	4.9853314101254174E-5	0.0	4.985331410125417E-6	0.21025884488774454	4.985331410125417E-6
24-25	0.04078499626623604	5.483864551137959E-5	0.0	4.985331410125417E-6	0.21618889660008872	4.985331410125417E-6
26-27	0.04483308537125788	5.982397692150501E-5	0.0	4.985331410125417E-6	0.220969829422399	4.985331410125417E-6
28-29	0.05448717964696575	6.231664262656771E-5	0.0	4.985331410125417E-6	0.22500296253319046	4.985331410125417E-6
30-31	0.0753582695954558	6.480930833163043E-5	0.0	4.985331410125417E-6	0.22954210678210968	4.985331410125417E-6
32-33	0.09367188453055153	7.477997115188127E-5	0.0	4.985331410125417E-6	0.23369987317815427	4.985331410125417E-6
34-35	0.10775544576415583	7.477997115188127E-5	0.0	4.985331410125417E-6	0.2372544144735737	4.985331410125417E-6
36-37	0.11062201132497795	7.477997115188127E-5	0.0	4.985331410125417E-6	0.2397196608558807	4.985331410125417E-6
38-39	0.1132791929665748	7.727263685694397E-5	0.0	4.985331410125417E-6	0.24175866140262198	4.985331410125417E-6
40-41	0.11497919097742756	7.976530256200668E-5	0.0	4.985331410125417E-6	0.24335895278527225	7.477997115188126E-6
42-43	0.11527831086203509	8.225796826706938E-5	0.0	4.985331410125417E-6	0.24497669282785794	9.970662820250835E-6
44-45	0.11601863257643871	8.47506339721321E-5	0.0	4.985331410125417E-6	0.24641496093967913	9.970662820250835E-6
46-47	0.11744693002543966	8.973596538225751E-5	0.0	4.985331410125417E-6	0.24786569238002565	9.970662820250835E-6
48-49	0.11871818953502163	9.222863108732023E-5	0.0	4.985331410125417E-6	0.2491045472354418	9.970662820250835E-6
50-51	0.11974267513980241	9.472129679238294E-5	0.0	4.985331410125417E-6	0.25045058671617565	9.970662820250835E-6
52-53	0.12074472675323761	9.472129679238294E-5	0.0	4.985331410125417E-6	0.25178167020267916	9.970662820250835E-6
54-55	0.12120337724296915	1.0469195961263377E-4	0.0	4.985331410125417E-6	0.252888413775727	9.970662820250835E-6
56-57	0.12201100093140946	1.1964795384301002E-4	0.0	4.985331410125417E-6	0.25420204860229506	9.970662820250835E-6
58-59	0.12218798019646893	1.2214061954807272E-4	0.0	4.985331410125417E-6	0.2553561528237391	9.970662820250835E-6
60-61	0.12241481277562963	1.2463328525313543E-4	0.0	7.477997115188126E-6	0.2562634831403819	9.970662820250835E-6
62-63	0.1225843140435739	1.2463328525313543E-4	0.0	9.970662820250835E-6	0.25736773404772467	9.970662820250835E-6
64-65	0.12260674803491946	1.3460394807338625E-4	0.0	9.970662820250835E-6	0.2586240375630763	9.970662820250835E-6
66-67	0.12266906467754603	1.445746108936371E-4	0.0	9.970662820250835E-6	0.2594516025771571	9.970662820250835E-6
68-69	0.12272390332305741	1.4955994230376253E-4	0.0	9.970662820250835E-6	0.2603016015825835	1.4955994230376252E-5
70-71	0.12284604394260548	1.4955994230376253E-4	0.0	1.2463328525313543E-5	0.261076820616858	1.4955994230376252E-5
72-73	0.1229307945765776	1.4955994230376253E-4	0.0	1.4955994230376252E-5	0.2617897230085059	2.2433991345564378E-5
74-75	0.12296319923074342	1.5703793941895065E-4	0.0	1.4955994230376252E-5	0.2627020386565589	2.4926657050627087E-5
76-77	0.12299560388490924	1.670086022392015E-4	0.0	1.4955994230376252E-5	0.26363180296454725	2.4926657050627087E-5
78-79	0.1230454571990105	1.695012679442642E-4	0.0	1.4955994230376252E-5	0.26473356120618496	2.7419322755689796E-5
80-81	0.12307786185317632	1.8445726217464044E-4	4.985331410125417E-6	1.4955994230376252E-5	0.26563092086000756	2.9911988460752504E-5
82-83	0.12310777384163707	1.8944259358476587E-4	4.985331410125417E-6	1.4955994230376252E-5	0.26643854454844784	2.9911988460752504E-5
84-85	0.1231576271557383	1.9193525928982858E-4	4.985331410125417E-6	1.4955994230376252E-5	0.2673309188708603	2.9911988460752504E-5
86-87	0.12318255381278895	1.9442792499489128E-4	4.985331410125417E-6	1.4955994230376252E-5	0.26824821985032343	2.9911988460752504E-5
88-89	0.12319750980701932	1.9442792499489128E-4	4.985331410125417E-6	1.4955994230376252E-5	0.2692851687836295	3.7389985575940634E-5
90-91	0.1232124658012497	2.043985878151421E-4	4.985331410125417E-6	1.4955994230376252E-5	0.26998810051245714	3.988265128100334E-5
92-93	0.1232124658012497	2.043985878151421E-4	4.985331410125417E-6	1.4955994230376252E-5	0.27062622293295324	4.2375316986066044E-5
94-95	0.1232324071268902	2.043985878151421E-4	4.985331410125417E-6	1.4955994230376252E-5	0.2713216766646657	4.4867982691128756E-5
96-97	0.12323739245830032	2.1187658493033025E-4	4.985331410125417E-6	1.4955994230376252E-5	0.2719971890707377	5.483864551137959E-5
98-99	0.12323739245830032	2.1436925063539295E-4	4.985331410125417E-6	1.4955994230376252E-5	0.27279234943065267	6.231664262656771E-5
100-101	0.12325484111823576	2.1436925063539295E-4	4.985331410125417E-6	1.4955994230376252E-5	0.2733681552085222	6.480930833163043E-5
102-103	0.12326730444676107	2.1935458204551836E-4	4.985331410125417E-6	2.2433991345564378E-5	0.2740062776290183	6.480930833163043E-5
104-105	0.12328475310669651	2.2433991345564377E-4	4.985331410125417E-6	2.4926657050627087E-5	0.27472167268637127	6.480930833163043E-5
106-107	0.12330718709804209	2.3181791057083192E-4	4.985331410125417E-6	2.4926657050627087E-5	0.27522519115879396	6.480930833163043E-5
108-109	0.1233321137550927	2.3431057627589462E-4	4.985331410125417E-6	2.4926657050627087E-5	0.27574366562544694	6.979463974175584E-5
110-111	0.12336202574355346	2.3431057627589462E-4	4.985331410125417E-6	2.4926657050627087E-5	0.27634938339177717	6.979463974175584E-5
112-113	0.12338695240060407	2.3431057627589462E-4	4.985331410125417E-6	2.4926657050627087E-5	0.27702489579784917	6.979463974175584E-5
114-115	0.12341686438906484	2.3431057627589462E-4	4.985331410125417E-6	2.4926657050627087E-5	0.2776879448753959	6.979463974175584E-5
116-117	0.12342434238618002	2.3929590768602003E-4	4.985331410125417E-6	2.4926657050627087E-5	0.27828618464461097	7.228730544681856E-5
118-119	0.12343182038329521	2.4428123909614544E-4	4.985331410125417E-6	2.4926657050627087E-5	0.27891433640228674	7.477997115188127E-5
120-121	0.12343182038329521	2.4428123909614544E-4	4.985331410125417E-6	2.9911988460752504E-5	0.2795798781455385	7.727263685694397E-5
122-123	0.12344179104611547	2.4428123909614544E-4	4.985331410125417E-6	2.9911988460752504E-5	0.2802204932317396	7.976530256200668E-5
124-125	0.12344926904323066	2.467739048012082E-4	4.985331410125417E-6	2.9911988460752504E-5	0.2808810496435812	8.973596538225751E-5
126-127	0.12347419570028129	2.4926657050627085E-4	4.985331410125417E-6	2.9911988460752504E-5	0.28157401070958865	9.222863108732023E-5
128-129	0.12347668836598634	2.4926657050627085E-4	4.985331410125417E-6	3.489731987087792E-5	0.2823716637352087	1.0469195961263377E-4
130-131	0.12347918103169139	2.4926657050627085E-4	4.985331410125417E-6	3.489731987087792E-5	0.2832141847435199	1.0469195961263377E-4
132-133	0.12349413702592177	2.4926657050627085E-4	4.985331410125417E-6	3.489731987087792E-5	0.2838722484896565	1.0469195961263377E-4
134-135	0.12350161502303697	2.4926657050627085E-4	7.477997115188126E-6	3.489731987087792E-5	0.28450787824444745	1.0469195961263377E-4
136-137	0.12350161502303697	2.4926657050627085E-4	9.970662820250835E-6	3.489731987087792E-5	0.2850986400165473	1.0469195961263377E-4
138-139	0.12350161502303697	2.4926657050627085E-4	9.970662820250835E-6	3.489731987087792E-5	0.2856993724514674	1.0967729102275918E-4
>>END_MODULE
