##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.lowPi.4d.S.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24025985
Total Bases	3.5 Gbp
Sequences flagged as poor quality	0
Sequence length	65-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.17758044051055	37.0	35.0	38.0	33.0	39.0
2	35.529287019866196	35.0	34.0	38.0	33.0	39.0
3	35.62970275724388	36.0	35.0	37.0	33.0	39.0
4	35.727163277592986	36.0	35.0	38.0	33.0	39.0
5	35.6642035279719	36.0	35.0	38.0	33.0	39.0
6	35.59380716336916	36.0	35.0	37.0	33.0	39.0
7	35.56189508983711	35.0	34.0	38.0	33.0	39.0
8	35.702917944883424	36.0	35.0	38.0	33.0	39.0
9	35.696408034883895	36.0	35.0	38.0	33.0	39.0
10-14	35.616965248251006	36.0	35.0	37.8	33.0	39.0
15-19	35.62404255226164	36.0	35.0	38.0	33.0	39.0
20-24	35.60820845430479	36.0	35.0	38.0	33.0	39.0
25-29	35.59205552654761	36.0	35.0	38.0	33.0	39.0
30-34	35.567378369710966	36.0	35.0	38.0	33.0	39.0
35-39	35.543181226492905	36.0	35.0	38.0	33.0	39.0
40-44	35.5248706015591	36.0	35.0	38.0	33.0	39.0
45-49	35.491489801562764	36.0	34.2	38.0	33.0	39.0
50-54	35.474878053907055	36.0	34.0	38.0	33.0	39.0
55-59	35.43798121908425	36.0	34.0	38.0	33.0	39.0
60-64	35.400065420834984	36.0	34.0	38.0	32.8	39.0
65-69	35.36318661837202	36.0	34.0	38.0	32.0	39.0
70-74	35.331205298346084	36.0	34.0	38.0	32.0	39.0
75-79	35.280295140094346	36.0	34.0	38.0	32.0	39.0
80-84	35.23746634545317	36.0	34.0	38.0	32.0	39.0
85-89	35.192647904583225	36.0	34.0	38.0	32.0	39.0
90-94	35.13750569374312	36.0	34.0	38.0	32.0	39.0
95-99	35.06930889623989	36.0	34.0	37.8	32.0	39.0
100-104	35.0146858295213	36.0	34.0	37.6	31.6	39.0
105-109	34.94874317927517	35.6	34.0	37.0	31.0	39.0
110-114	34.874353286703	35.0	34.0	37.0	31.0	39.0
115-119	34.79846169451984	35.0	34.0	37.0	31.0	39.0
120-124	34.71776927431593	35.0	34.0	37.0	30.8	39.0
125-129	34.65260020530268	35.0	34.0	37.0	30.0	39.0
130-134	34.55677054940078	35.0	34.0	37.0	30.0	39.0
135-139	34.46014278224388	35.0	34.0	37.0	30.0	39.0
140-144	34.365528473417534	35.0	34.0	37.0	30.0	39.0
145-149	34.325027182015184	35.0	34.0	37.0	29.4	39.0
150	35.056421635036585	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	9.0
21	141.0
22	1288.0
23	7471.0
24	27502.0
25	68332.0
26	134274.0
27	223712.0
28	325840.0
29	430594.0
30	559135.0
31	739270.0
32	1007760.0
33	1448946.0
34	2270276.0
35	4047254.0
36	1.0674199E7
37	2059981.0
38	1.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.96308559253658	19.89237069780906	15.73075567973592	31.41378802991844
2	24.799999833513557	26.194565216486488	31.571702798387413	17.433732151612546
3	20.244223077638647	29.666696287373856	29.753764517875126	20.335316117112367
4	23.595982582856962	34.01265479907087	21.968053420829715	20.423309197242453
5	25.63268061642426	35.08853018929297	21.804354743416347	17.474434450866426
6	19.40387043444837	39.39301135832724	21.846575697104615	19.356542510119773
7	20.675347961800526	21.37039126595642	36.75336515859808	21.200895613644978
8	21.658268883316868	24.034625347066964	28.003936405016187	26.30316936459998
9	23.757584601536784	23.630397292397873	27.836660328805706	24.775357777259636
10-14	23.151923142494006	29.31128497151077	25.157020643079203	22.37977124291602
15-19	23.260685462011235	28.0683901201137	26.653769241927023	22.01715517594804
20-24	23.124796949009973	28.104055072905897	26.620177500486893	22.15097047759724
25-29	23.28259340876139	27.68020707579731	26.615030351513163	22.42216916392814
30-34	23.12218810693662	27.389193220500164	26.841414633709416	22.6472040388538
35-39	23.427303396718177	27.107133380795833	26.84001592442516	22.625547298060827
40-44	23.563431010216647	26.774746592075203	26.95791577327631	22.703906624431838
45-49	23.48659689487525	26.6211755865258	27.052723349762548	22.8395041688364
50-54	23.648496167341225	26.58223901951755	26.83901434648397	22.93025046665726
55-59	23.742272377178292	26.53502697183903	26.87886802559812	22.843832625384557
60-64	23.506028356431823	26.505002200783878	26.929545048242936	23.059424394541363
65-69	23.652783299693226	26.476984129673014	26.860313300984412	23.00991926964935
70-74	23.856022935713835	26.40629819784351	26.65654240025061	23.08113646619205
75-79	23.71058661375321	26.55451259431636	26.824994316145386	22.909906475785046
80-84	23.98185048755881	26.534516398751922	26.53583830543949	22.94779480824978
85-89	23.82171073414586	26.616107687244806	26.684689715456962	22.87749186315237
90-94	23.810704528726635	26.43011164755678	26.670712221485253	23.088471602231337
95-99	23.792100146261706	26.66974333397329	26.702441838222335	22.835714681542665
100-104	24.028476194144538	26.409634052545194	26.689401102220987	22.872488651089277
105-109	23.832805741791198	26.596203713416543	26.666825722621073	22.904164822171182
110-114	23.929196307755586	26.693349807005763	26.554604603224647	22.82284928201401
115-119	24.02731308210387	26.693740547364687	26.70110722917569	22.577839141355753
120-124	23.93518122731563	26.752622685554783	26.91739132725982	22.394804759869764
125-129	23.948672393440862	26.865341238565303	26.733273415476678	22.452712952517157
130-134	24.11720140771877	26.85028678679021	26.690238212563294	22.342273592927725
135-139	23.91067180074954	26.908113987923933	26.892358459764814	22.288855751561716
140-144	23.922738394179007	27.03155364722882	26.752891673261033	22.292816285331146
145-149	23.722055834471966	27.333382064198368	26.519387668564654	22.425174432765008
150	24.073238144036253	26.118605551177044	26.716000729222795	23.092155575563915
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.5
2	8.0
3	33.0
4	48.5
5	47.5
6	46.0
7	34.0
8	27.0
9	23.0
10	15.0
11	12.0
12	13.0
13	15.0
14	25.0
15	44.5
16	67.0
17	120.5
18	221.0
19	426.0
20	655.0
21	991.5
22	1531.0
23	2313.0
24	3729.0
25	5812.0
26	8915.5
27	13773.5
28	20167.5
29	28193.0
30	39706.0
31	56502.0
32	82915.5
33	113332.0
34	148204.0
35	205962.5
36	292687.0
37	392254.5
38	508737.0
39	661386.0
40	841688.0
41	1030016.5
42	1219735.5
43	1400910.0
44	1537676.0
45	1614908.5
46	1639147.5
47	1599079.0
48	1529208.5
49	1464513.5
50	1350988.5
51	1182573.0
52	1002002.5
53	829998.0
54	680703.5
55	564185.0
56	465727.0
57	383698.0
58	319111.0
59	257390.5
60	193750.5
61	131592.0
62	82533.0
63	50057.0
64	30333.0
65	15648.5
66	8609.0
67	4575.5
68	2405.5
69	1410.5
70	895.0
71	670.0
72	418.0
73	316.5
74	253.0
75	163.5
76	111.0
77	40.0
78	11.0
79	4.0
80	2.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.0810801305336702E-5
3	0.0
4	4.16216026106734E-6
5	0.0
6	0.0
7	0.0
8	8.32432052213468E-6
9	1.248648078320202E-5
10-14	0.09902028990694865
15-19	0.0
20-24	8.324320522134681E-7
25-29	0.0
30-34	8.324320522134681E-7
35-39	0.0
40-44	0.0
45-49	8.324320522134681E-7
50-54	2.497296156640404E-6
55-59	0.0
60-64	8.324320522134681E-7
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	6.659572723312476E-6
100-104	8.324542061946448E-7
105-109	8.324631180129186E-7
110-114	8.32474545654748E-7
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
64-65	1.0
66-67	3.0
68-69	2.0
70-71	10.0
72-73	3.0
74-75	11.0
76-77	13.0
78-79	14.0
80-81	25.0
82-83	28.0
84-85	31.0
86-87	34.0
88-89	43.0
90-91	60.0
92-93	47.0
94-95	62.0
96-97	61.0
98-99	90.0
100-101	107.0
102-103	92.0
104-105	105.0
106-107	110.0
108-109	131.0
110-111	137.0
112-113	153.0
114-115	138.0
116-117	172.0
118-119	190.0
120-121	237.0
122-123	267.0
124-125	306.0
126-127	359.0
128-129	421.0
130-131	508.0
132-133	720.0
134-135	919.0
136-137	1488.0
138-139	4324.0
140-141	8577.0
142-143	9168.0
144-145	59536.0
146-147	795080.0
148-149	904828.0
150-151	2.2237374E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.35568947818124
#Duplication Level	Percentage of total
1	21.794902241424456
2	9.224839311956314
3	5.835341363936357
4	4.221278648508512
5	3.2052477358939258
6	2.516812887379991
7	2.122158961861765
8	1.845922925450096
9	1.4944818366605606
>10	19.005896011996555
>50	6.572103309234367
>100	13.253828574851582
>500	3.7555514145701623
>1k	4.364826338859082
>5k	0.6054887780583952
>10k+	0.1813196593577537
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.412971414075219E-4	8.32432052213468E-6	0.0	0.0	0.003229836362588256	0.0
2	0.001539999296594916	1.664864104426936E-5	0.0	0.0	0.012369940295892135	0.0
3	0.0024057286308969226	1.664864104426936E-5	0.0	0.0	0.016890046339411265	0.0
4	0.006076753981158317	2.0810801305336702E-5	0.0	0.0	0.02294182735900318	0.0
5	0.006309834955778088	2.0810801305336702E-5	0.0	0.0	0.02710398762007052	0.0
6	0.0074585911878326736	2.0810801305336702E-5	0.0	4.16216026106734E-6	0.034853930026177905	0.0
7	0.009722806369853306	2.497296156640404E-5	0.0	4.16216026106734E-6	0.04498046594135475	0.0
8	0.016403073588866387	2.497296156640404E-5	0.0	4.16216026106734E-6	0.061088026151685355	0.0
9	0.0171023165127257	2.497296156640404E-5	0.0	4.16216026106734E-6	0.07281283160711205	0.0
10-11	0.017741208112799535	2.497296156640404E-5	0.0	6.24324039160101E-6	0.12301472759597577	0.0
12-13	0.01844669427705045	3.1216201958005055E-5	0.0	8.32432052213468E-6	0.14957763438210753	0.0
14-15	0.021006422837606866	3.745944234960606E-5	0.0	8.32432052213468E-6	0.1615105478505876	0.0
16-17	0.023788826972130382	4.1621602610673404E-5	0.0	8.32432052213468E-6	0.16896081471789814	0.0
18-19	0.029530527052272777	4.786484300227441E-5	0.0	8.32432052213468E-6	0.17682313545105433	0.0
20-21	0.03479357870239243	4.994592313280808E-5	0.0	8.32432052213468E-6	0.18233175455657696	0.0
22-23	0.03712438844859014	4.994592313280808E-5	0.0	8.32432052213468E-6	0.18793194118784307	0.0
24-25	0.04175271065889702	5.827024365494276E-5	0.0	8.32432052213468E-6	0.19320747931874593	0.0
26-27	0.04563600618247285	6.24324039160101E-5	0.0	8.32432052213468E-6	0.19730720717589728	0.0
28-29	0.05436197516980053	6.24324039160101E-5	2.08108013053367E-6	8.32432052213468E-6	0.20107188113203267	0.0
30-31	0.07398031756034143	6.659456417707744E-5	1.664864104426936E-5	8.32432052213468E-6	0.20523404139310003	0.0
32-33	0.093113768280468	6.659456417707744E-5	1.664864104426936E-5	8.32432052213468E-6	0.20911733691667583	0.0
34-35	0.10825362623010046	6.867564430761112E-5	1.664864104426936E-5	1.248648078320202E-5	0.21250533536918464	0.0
36-37	0.11133154374315975	7.075672443814478E-5	1.664864104426936E-5	1.248648078320202E-5	0.21493187480138692	0.0
38-39	0.11411186679755272	7.699996482974579E-5	1.664864104426936E-5	1.248648078320202E-5	0.2169963062908763	0.0
40-41	0.11565602825440871	8.740536548241414E-5	1.664864104426936E-5	1.248648078320202E-5	0.21890673785070622	0.0
42-43	0.11593073083163916	9.156752574348149E-5	1.664864104426936E-5	1.248648078320202E-5	0.22069854784309573	0.0
44-45	0.11669448723954501	9.364860587401515E-5	1.664864104426936E-5	1.248648078320202E-5	0.22208662829016168	0.0
46-47	0.1180409460840003	9.989184626561616E-5	1.664864104426936E-5	1.248648078320202E-5	0.22350176277892458	0.0
48-49	0.11921883743788236	1.0405400652668351E-4	1.664864104426936E-5	1.248648078320202E-5	0.2247462486969837	2.08108013053367E-6
50-51	0.1200928910927065	1.1237832704881819E-4	1.664864104426936E-5	1.248648078320202E-5	0.22594911301243215	4.16216026106734E-6
52-53	0.12097526906805278	1.1445940717935186E-4	1.664864104426936E-5	1.248648078320202E-5	0.22718527460996918	4.16216026106734E-6
54-55	0.12143102561663965	1.2070264757095286E-4	1.664864104426936E-5	1.248648078320202E-5	0.22826743627784668	4.16216026106734E-6
56-57	0.12216148474245697	1.248648078320202E-4	1.664864104426936E-5	1.248648078320202E-5	0.22946405735290354	4.16216026106734E-6
58-59	0.12230716035159434	1.248648078320202E-4	1.872972117480303E-5	1.248648078320202E-5	0.23052957037973676	4.16216026106734E-6
60-61	0.12256105212751944	1.2694588796255385E-4	2.497296156640404E-5	1.248648078320202E-5	0.23140986727495252	4.16216026106734E-6
62-63	0.1227629169001812	1.2902696809308754E-4	2.497296156640404E-5	1.248648078320202E-5	0.23248162354217736	4.16216026106734E-6
64-65	0.12277956554122546	1.3735128861522224E-4	2.705404169693771E-5	1.456756091373569E-5	0.2335471365690106	4.16216026106734E-6
66-67	0.12285240334579414	1.4983776939842425E-4	2.913512182747138E-5	1.664864104426936E-5	0.23436916322057139	4.16216026106734E-6
68-69	0.12289818710866589	1.539999296594916E-4	2.913512182747138E-5	1.664864104426936E-5	0.23516829799069633	4.16216026106734E-6
70-71	0.12301888975623684	1.539999296594916E-4	2.913512182747138E-5	1.664864104426936E-5	0.23593829763899377	4.16216026106734E-6
72-73	0.12305843027871699	1.539999296594916E-4	2.913512182747138E-5	1.664864104426936E-5	0.23655637843776228	8.32432052213468E-6
74-75	0.12307924108002231	1.539999296594916E-4	2.913512182747138E-5	1.664864104426936E-5	0.237374242929062	1.248648078320202E-5
76-77	0.12311045728198033	1.664864104426936E-4	2.913512182747138E-5	2.0810801305336702E-5	0.23821499930179763	1.664864104426936E-5
78-79	0.12317288968589633	1.810539713564293E-4	2.913512182747138E-5	2.0810801305336702E-5	0.23930340421006674	1.872972117480303E-5
80-81	0.12321451128850701	1.8313505148696298E-4	3.329728208853872E-5	2.0810801305336702E-5	0.24007548493849473	2.497296156640404E-5
82-83	0.1232332410096818	1.8313505148696298E-4	3.329728208853872E-5	2.497296156640404E-5	0.24086837646822806	2.497296156640404E-5
84-85	0.12326861937190088	1.872972117480303E-4	3.329728208853872E-5	2.913512182747138E-5	0.24173826796279113	2.913512182747138E-5
86-87	0.12329775449372835	1.9145937200909765E-4	3.329728208853872E-5	2.913512182747138E-5	0.24259151081630992	3.1216201958005055E-5
88-89	0.1233310517758169	1.9145937200909765E-4	3.329728208853872E-5	2.913512182747138E-5	0.24359251035909663	3.329728208853872E-5
90-91	0.12335186257712223	1.9145937200909765E-4	3.329728208853872E-5	2.913512182747138E-5	0.24432505056504447	3.745944234960606E-5
92-93	0.12336643013803597	1.9145937200909765E-4	3.329728208853872E-5	2.913512182747138E-5	0.2449868340465542	3.745944234960606E-5
94-95	0.1233997274201245	1.9145937200909765E-4	3.745944234960606E-5	3.329728208853872E-5	0.24570064453132723	3.745944234960606E-5
96-97	0.1233997274201245	1.935404521396313E-4	3.745944234960606E-5	3.329728208853872E-5	0.24643942797766666	4.1621602610673404E-5
98-99	0.12340805174064665	2.0394585279229967E-4	3.745944234960606E-5	3.329728208853872E-5	0.24708040065787107	4.786484300227441E-5
100-101	0.12342053822142984	2.0394585279229967E-4	3.745944234960606E-5	3.329728208853872E-5	0.24770472469703114	5.618916352440909E-5
102-103	0.12343510578234357	2.0394585279229967E-4	4.1621602610673404E-5	3.329728208853872E-5	0.24830823793488593	5.827024365494276E-5
104-105	0.12347048414456264	2.0394585279229967E-4	4.5783762871740746E-5	3.329728208853872E-5	0.24895753493561243	5.827024365494276E-5
106-107	0.12347880846508479	2.0394585279229967E-4	4.5783762871740746E-5	3.537836221907239E-5	0.2495360752119008	6.24324039160101E-5
108-109	0.12350794358691225	2.0394585279229967E-4	4.5783762871740746E-5	3.745944234960606E-5	0.25020410193380205	6.24324039160101E-5
110-111	0.12353083546834812	2.0810801305336702E-4	4.5783762871740746E-5	3.745944234960606E-5	0.25083883137361485	6.24324039160101E-5
112-113	0.12354956518952293	2.101890931839007E-4	5.410808339387542E-5	3.745944234960606E-5	0.2515110202557772	6.24324039160101E-5
114-115	0.12357453815108933	2.1227017331443436E-4	5.410808339387542E-5	3.745944234960606E-5	0.2521644794167648	6.24324039160101E-5
116-117	0.12358910571200307	2.1227017331443436E-4	5.410808339387542E-5	3.745944234960606E-5	0.2527388575327921	6.451348404654377E-5
118-119	0.1235911867921336	2.1643233357550168E-4	5.827024365494276E-5	3.954052248013974E-5	0.2533236410494721	7.075672443814478E-5
120-121	0.1235911867921336	2.1643233357550168E-4	6.24324039160101E-5	4.1621602610673404E-5	0.2539999920918955	7.908104496027946E-5
122-123	0.12359326787226413	2.1643233357550168E-4	6.24324039160101E-5	4.1621602610673404E-5	0.25461391073040296	7.908104496027946E-5
124-125	0.1236036732729168	2.1643233357550168E-4	6.24324039160101E-5	4.1621602610673404E-5	0.25530691041387066	8.740536548241414E-5
126-127	0.1236286462344832	2.1643233357550168E-4	6.24324039160101E-5	4.1621602610673404E-5	0.25597285605564146	8.740536548241414E-5
128-129	0.12363280839474428	2.1643233357550168E-4	6.24324039160101E-5	4.1621602610673404E-5	0.2566179908961069	9.156752574348149E-5
130-131	0.1236411327152664	2.2059449383656903E-4	6.24324039160101E-5	4.1621602610673404E-5	0.25732763922061885	9.156752574348149E-5
132-133	0.12365153811591909	2.2475665409763638E-4	6.24324039160101E-5	4.1621602610673404E-5	0.258014395663695	9.364860587401515E-5
134-135	0.12366402459670228	2.2475665409763638E-4	7.075672443814478E-5	4.1621602610673404E-5	0.2586116656611581	9.989184626561616E-5
136-137	0.12367442999735495	2.2683773422817004E-4	7.075672443814478E-5	4.1621602610673404E-5	0.25922974645992664	1.0405400652668351E-4
138-139	0.12367859215761602	2.289188143587037E-4	7.075672443814478E-5	4.370268274120707E-5	0.25985823265934777	1.0821616678775084E-4
>>END_MODULE
