##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.control.2d.R.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24210249
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	56-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.466229983838666	37.0	35.0	39.0	34.0	39.0
2	35.518440847097445	35.0	34.0	37.0	33.0	39.0
3	35.643241504868456	36.0	35.0	37.0	33.0	39.0
4	35.78638893800721	36.0	35.0	38.0	34.0	39.0
5	35.72784241087318	36.0	35.0	38.0	33.0	39.0
6	35.575986517115126	36.0	35.0	37.0	33.0	39.0
7	35.61167301501112	35.0	34.0	38.0	33.0	39.0
8	35.785278044847864	36.0	35.0	38.0	33.0	39.0
9	35.789826986083455	36.0	35.0	38.0	33.0	39.0
10-14	35.76021945912246	36.0	35.0	37.8	33.0	39.0
15-19	35.730067121573185	36.0	35.0	38.0	33.0	39.0
20-24	35.70502515690772	36.0	35.0	38.0	33.0	39.0
25-29	35.68707836916506	36.0	35.0	38.0	33.0	39.0
30-34	35.66640802413887	36.0	35.0	38.0	33.0	39.0
35-39	35.64402214120144	36.0	35.0	38.0	33.0	39.0
40-44	35.62355348761593	36.0	35.0	38.0	33.0	39.0
45-49	35.5941696840871	36.0	35.0	38.0	33.0	39.0
50-54	35.573195352100676	36.0	35.0	38.0	33.0	39.0
55-59	35.53871317556735	36.0	35.0	38.0	33.0	39.0
60-64	35.513032753726954	36.0	35.0	38.0	33.0	39.0
65-69	35.475463712214506	36.0	34.2	38.0	33.0	39.0
70-74	35.447540819460414	36.0	34.0	38.0	33.0	39.0
75-79	35.40554931708843	36.0	34.0	38.0	32.0	39.0
80-84	35.353888108768295	36.0	34.0	38.0	32.0	39.0
85-89	35.31792058323912	36.0	34.0	38.0	32.0	39.0
90-94	35.275607050312814	36.0	34.0	38.0	32.0	39.0
95-99	35.21539392303113	36.0	34.0	38.0	32.0	39.0
100-104	35.162066844936234	36.0	34.0	38.0	32.0	39.0
105-109	35.0967119199767	36.0	34.0	38.0	32.0	39.0
110-114	35.03995179648768	36.0	34.0	38.0	31.4	39.0
115-119	34.98260381136814	36.0	34.0	38.0	31.0	39.0
120-124	34.90797276824233	36.0	34.0	38.0	31.0	39.0
125-129	34.842562750766504	35.4	34.0	38.0	31.0	39.0
130-134	34.76960841912479	35.0	34.0	38.0	30.8	39.0
135-139	34.685378947633886	35.0	34.0	37.0	30.0	39.0
140-144	34.577606729415166	35.0	34.0	37.0	30.0	39.0
145-149	34.5508353500625	35.0	34.0	37.0	30.0	39.0
150	35.23354751038706	36.0	34.0	38.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	135.0
22	1242.0
23	6324.0
24	22898.0
25	57890.0
26	119810.0
27	213838.0
28	316974.0
29	414828.0
30	528452.0
31	687994.0
32	923743.0
33	1311866.0
34	2041396.0
35	3690750.0
36	1.1514819E7
37	2357275.0
38	8.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.370032501524456	20.061511965449014	11.973850413517019	38.59460511950951
2	24.200193066994068	24.66434360092703	37.357785952552575	13.777677379526331
3	16.75217797222986	29.96454931132679	31.37541047182125	21.907862244622102
4	21.050287421661793	34.751303053512586	23.546767321558733	20.65164220326689
5	25.080964677397578	35.32436200883353	23.01607472108197	16.578598592686923
6	17.901835706026816	38.47442874296749	24.417563817703815	19.206171733301876
7	19.097445053126055	20.54589773116336	39.66100472572587	20.695652489984717
8	19.23084723333494	22.295536902573783	31.318793953750745	27.15482191034053
9	22.403726620077308	22.52679846456763	30.716049223616	24.353425691739066
10-14	22.87289238536952	29.025941864538446	26.029281235397455	22.07188451469458
15-19	22.90245341962406	28.403204774969478	27.133803539154016	21.560538266252447
20-24	23.038701119446536	28.366642069435144	27.04031573463202	21.554341076486295
25-29	23.03482380540572	28.50324257301112	27.010768869002543	21.451164752580613
30-34	23.16252096374556	28.22960391691965	27.02689096671414	21.58098415262065
35-39	23.24374772023204	28.234688540378084	27.052890699306726	21.468673040083146
40-44	23.415446904325517	27.945401965919476	27.161026720543024	21.478124409211983
45-49	23.448318933027082	27.81670688310558	27.196197775578433	21.538776408288903
50-54	23.55631699616142	27.682709087378655	27.196721520708028	21.564252395751897
55-59	23.681807411773605	27.662994155813784	27.065911475736865	21.589286956675753
60-64	23.657748134719615	27.625361419269417	27.096254893320467	21.620635552690505
65-69	23.746120030843144	27.501978088283618	27.087631169681725	21.66427071119151
70-74	23.766482781892694	27.489244878661562	27.00010709520787	21.744165244237877
75-79	23.750337782045374	27.550634964983388	27.04177045961679	21.657256793354446
80-84	23.85926082591378	27.580837103354742	26.94833000945913	21.61157206127234
85-89	23.866232224022223	27.4992077434966	26.98654523301982	21.648014799461357
90-94	23.800954405192684	27.453020992306172	27.069284170991605	21.67674043150954
95-99	23.932200721032164	27.572001706275078	26.99906371314917	21.49673385954359
100-104	24.044353954509628	27.616755686123064	26.829997912929443	21.508892446437866
105-109	23.847428058576988	27.742691972639992	26.88538160726971	21.52449836151331
110-114	23.854564219680015	27.713314303319226	26.877494775043605	21.554626701957158
115-119	23.953179409036636	27.743912846960793	26.835193481506494	21.46771426249608
120-124	23.927182891528247	27.654523478416298	27.034491028695896	21.383802601359562
125-129	23.91284473198828	27.690223537575488	27.02493880981259	21.371992920623644
130-134	23.95113686760185	27.63220736643931	27.095516583696934	21.32113918226191
135-139	23.884140342058	27.659374452740188	27.169013960203216	21.287471244998596
140-144	23.933106945328202	27.860488670864804	27.131066733285902	21.075337650521092
145-149	23.69634511895626	27.940008003670414	27.121371696324243	21.242275181049084
150	24.298088388323997	26.46774822135428	27.32922121723339	21.904942173088333
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.5
2	3.0
3	6.5
4	9.0
5	10.0
6	12.5
7	13.0
8	11.0
9	10.0
10	7.5
11	7.5
12	18.0
13	28.5
14	41.0
15	68.5
16	120.0
17	207.0
18	341.0
19	646.0
20	1028.0
21	1423.5
22	2112.5
23	3346.0
24	5211.5
25	8534.0
26	13597.0
27	20625.0
28	32766.0
29	50158.0
30	65947.5
31	84130.5
32	109362.0
33	141850.0
34	190763.5
35	262514.0
36	361390.5
37	485241.5
38	634564.0
39	820779.5
40	1034051.5
41	1233541.5
42	1423915.5
43	1597872.0
44	1712124.0
45	1764273.5
46	1758323.5
47	1697279.5
48	1580348.5
49	1424709.5
50	1240465.0
51	1038182.0
52	843954.0
53	667300.5
54	517165.5
55	390444.0
56	289149.0
57	212480.0
58	152034.5
59	108141.5
60	74543.5
61	48796.0
62	32421.5
63	22486.0
64	15252.5
65	10062.0
66	7092.0
67	5312.0
68	4500.5
69	3138.0
70	1549.5
71	907.5
72	485.0
73	388.0
74	344.5
75	217.5
76	144.5
77	47.0
78	10.5
79	4.5
80	3.5
81	2.0
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.009812373263901582
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	8.260965827019623E-7
70-74	1.6521938614884927E-6
75-79	2.2222045987904597E-4
80-84	0.019488555085597558
85-89	8.261061983309814E-7
90-94	0.0
95-99	8.261233009184029E-7
100-104	8.261372510392022E-7
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	8.262806697619581E-7
135-139	0.0
140-144	9.011529168534148E-5
145-149	0.009891255293122065
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
56-57	1.0
58-59	0.0
60-61	0.0
62-63	2.0
64-65	0.0
66-67	3.0
68-69	3.0
70-71	5.0
72-73	10.0
74-75	19.0
76-77	26.0
78-79	33.0
80-81	40.0
82-83	47.0
84-85	50.0
86-87	87.0
88-89	104.0
90-91	107.0
92-93	87.0
94-95	91.0
96-97	143.0
98-99	143.0
100-101	207.0
102-103	170.0
104-105	162.0
106-107	190.0
108-109	176.0
110-111	205.0
112-113	187.0
114-115	215.0
116-117	247.0
118-119	269.0
120-121	289.0
122-123	300.0
124-125	332.0
126-127	410.0
128-129	454.0
130-131	547.0
132-133	691.0
134-135	988.0
136-137	1387.0
138-139	3208.0
140-141	6682.0
142-143	9890.0
144-145	65130.0
146-147	860772.0
148-149	855670.0
150-151	2.240047E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.88830992928625
#Duplication Level	Percentage of total
1	23.906435092244415
2	11.012830078751906
3	7.526103384590536
4	5.419899941995519
5	4.086350402206784
6	3.2955324599868963
7	2.71791186415151
8	2.2809378518472556
9	1.957043283067122
>10	22.436002729151248
>50	5.783017864318031
>100	7.022418711208149
>500	1.224045949180565
>1k	1.1564355376288404
>5k	0.03101472516931503
>10k+	0.14402012450174112
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.130482094587296E-6	8.260964189174593E-6	0.0	0.0	7.476172591203006E-4	0.0
2	1.2391446283761889E-5	2.0652410472936482E-5	0.0	0.0	0.004659183802694471	0.0
3	4.1304820945872964E-5	2.4782892567523778E-5	0.0	4.130482094587296E-6	0.005985068555056992	0.0
4	7.434867770257133E-5	2.8913374662111075E-5	0.0	4.130482094587296E-6	0.007480303073297594	0.0
5	7.847915979715864E-5	2.8913374662111075E-5	0.0	4.130482094587296E-6	0.00902923385876783	0.0
6	8.674012398633323E-5	2.8913374662111075E-5	0.0	4.130482094587296E-6	0.010929255622277987	0.0
7	9.087060608092052E-5	5.782674932422215E-5	0.0	4.130482094587296E-6	0.01292427847396365	0.0
8	1.3217542702679348E-4	5.782674932422215E-5	0.0	4.130482094587296E-6	0.018475646409088976	0.0
9	1.4456687331055538E-4	6.195723141880945E-5	0.0	4.130482094587296E-6	0.02462593424792946	0.0
10-11	1.5489307854702362E-4	6.195723141880945E-5	0.0	4.130482094587296E-6	0.03077415684572265	0.0
12-13	1.9619789949289657E-4	8.260964189174593E-5	0.0	8.260964189174593E-6	0.036563027501286746	0.0
14-15	2.189155510131267E-4	9.087060608092052E-5	0.0	8.260964189174593E-6	0.04461333710363739	0.0
16-17	2.313069972968886E-4	9.087060608092052E-5	4.130482094587296E-6	8.260964189174593E-6	0.0500056814781211	0.0
18-19	3.077209160467536E-4	9.087060608092052E-5	8.260964189174593E-6	8.260964189174593E-6	0.05724435134888534	0.0
20-21	3.510909780399202E-4	9.913157027009511E-5	8.260964189174593E-6	8.260964189174593E-6	0.06261397807184882	0.0
22-23	4.047872452695551E-4	1.197839807430316E-4	2.0652410472936482E-5	1.2391446283761889E-5	0.07024917422369345	0.0
24-25	4.543530304046026E-4	1.280449449322062E-4	2.0652410472936482E-5	1.2391446283761889E-5	0.07561260522351504	0.0
26-27	4.894621282085946E-4	1.3630590912138077E-4	2.0652410472936482E-5	1.2391446283761889E-5	0.0799537419049263	0.0
28-29	5.885936984786897E-4	1.3630590912138077E-4	2.0652410472936482E-5	1.2391446283761889E-5	0.08435477057670906	0.0
30-31	7.558782233094753E-4	1.3630590912138077E-4	2.271765152023013E-5	1.2391446283761889E-5	0.08920602179680184	0.0
32-33	8.963146145254434E-4	1.4456687331055538E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.09531913529679104	0.0
34-35	0.001016098595268475	1.4663211435784904E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.09872678302482557	0.0
36-37	0.001063599139356229	1.4869735540514267E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.10208899544981961	0.0
38-39	0.0011234911297277445	1.4869735540514267E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.1057362111393402	0.0
40-41	0.0011751221559100859	1.507625964524363E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.1086007004719365	2.065241047293648E-6
42-43	0.0011957745663830222	1.631540427361982E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.1112607309408507	4.130482094587296E-6
44-45	0.0012308836641870143	1.755454890199601E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.11364608435047488	4.130482094587296E-6
46-47	0.0012866451724639428	1.9826314054019023E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.11644035548746318	4.130482094587296E-6
48-49	0.0013547981270246334	2.0652410472936483E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.11889179661060074	4.130482094587296E-6
50-51	0.0014539296972947283	2.189155510131267E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.12084757988238783	4.130482094587296E-6
52-53	0.001538604580233768	2.2304603310771402E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.12277031929741822	4.130482094587296E-6
54-55	0.0016046922937471646	2.3750272043876954E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.12476534214910387	4.130482094587296E-6
56-57	0.0016831714535443234	2.436984435806505E-4	2.4782892567523778E-5	1.2391446283761889E-5	0.12668808156413425	6.1957231418809446E-6
58-59	0.0017575201312468947	2.4576368462794415E-4	2.6848133614817426E-5	1.6521928378349185E-5	0.12849310223946891	8.260964189174593E-6
60-61	0.0018463254962805215	2.7674230033734886E-4	2.8913374662111075E-5	1.6521928378349185E-5	0.12987887898220296	8.260964189174593E-6
62-63	0.0018855650761791009	2.8913374662111076E-4	2.8913374662111075E-5	1.6521928378349185E-5	0.1316880301396322	8.260964189174593E-6
64-65	0.0019062174866520372	2.99459951857579E-4	2.8913374662111075E-5	1.6521928378349185E-5	0.13331957056699417	1.0326205236468241E-5
66-67	0.00203219719053695	3.2424284442510273E-4	3.0978615709404726E-5	1.6521928378349185E-5	0.13475904357695784	1.2391446283761889E-5
68-69	0.002061110565199061	3.614171832763885E-4	3.304385675669837E-5	1.6521928378349185E-5	0.13605601495465824	1.2391446283761889E-5
70-71	0.00207763249357741	3.6761290641826934E-4	3.304385675669837E-5	1.6521928378349185E-5	0.13742526976901392	1.2391446283761889E-5
72-73	0.0021148068324286957	3.75873870607444E-4	3.304385675669837E-5	1.6521928378349185E-5	0.138410389748573	1.2391446283761889E-5
74-75	0.002137524483948926	3.8826531689120587E-4	3.304385675669837E-5	2.0652410472936482E-5	0.139556598529821	1.4456687331055537E-5
76-77	0.0022056774385096163	4.192439326006106E-4	3.304385675669837E-5	2.0652410472936482E-5	0.1409506362367442	1.6521928378349185E-5
78-79	0.002284156598306775	4.4609206621542804E-4	3.304385675669837E-5	2.0652410472936482E-5	0.14274326546579508	2.0652410472936482E-5
80-81	0.002311004731921592	4.5228778935730896E-4	3.304385675669837E-5	2.0652410472936482E-5	0.1440670849771103	2.0652410472936482E-5
82-83	0.002341983347630997	4.6467923564107085E-4	3.304385675669837E-5	2.271765152023013E-5	0.14583493131359365	2.0652410472936482E-5
84-85	0.0023894838917187513	4.6880971773565817E-4	3.304385675669837E-5	2.6848133614817426E-5	0.1473838620990639	2.4782892567523778E-5
86-87	0.0024266582305700365	4.7707068192483275E-4	3.717433885128567E-5	3.0978615709404726E-5	0.14922399187220256	2.4782892567523778E-5
88-89	0.0024473106410429733	4.8533164611400733E-4	3.717433885128567E-5	3.304385675669837E-5	0.15118803610817882	3.0978615709404726E-5
90-91	0.0024617673283740286	5.039188155396501E-4	3.717433885128567E-5	3.304385675669837E-5	0.15223717856020397	3.304385675669837E-5
92-93	0.0024679630515159097	5.121797797288248E-4	3.717433885128567E-5	3.304385675669837E-5	0.15320990709347931	3.5109097803992016E-5
94-95	0.002482419738846965	5.245712260125866E-4	3.717433885128567E-5	3.304385675669837E-5	0.15429622388435577	3.717433885128567E-5
96-97	0.0024968764261780207	5.287017081071739E-4	3.717433885128567E-5	3.5109097803992016E-5	0.15517808181155013	3.717433885128567E-5
98-99	0.0025361160060766	5.307669491544676E-4	3.717433885128567E-5	3.717433885128567E-5	0.1560289611230351	3.717433885128567E-5
100-101	0.002546442211313068	5.348974312490549E-4	3.717433885128567E-5	3.717433885128567E-5	0.15680342651577026	4.337006199316661E-5
102-103	0.0025505726934076556	5.369626722963486E-4	4.1304820945872964E-5	3.717433885128567E-5	0.15769974113029567	4.9565785135047556E-5
104-105	0.0025691598628332984	5.410931543909359E-4	4.337006199316661E-5	4.1304820945872964E-5	0.15855888140596985	4.9565785135047556E-5
106-107	0.0026393780584412826	5.493541185801104E-4	4.543530304046026E-5	4.1304820945872964E-5	0.15942421740478588	4.9565785135047556E-5
108-109	0.002655899986819632	5.658760469584596E-4	4.543530304046026E-5	4.1304820945872964E-5	0.16022966141323042	4.9565785135047556E-5
110-111	0.00268894384357633	5.741370111476342E-4	4.543530304046026E-5	4.1304820945872964E-5	0.16113010650985043	5.369626722963485E-5
112-113	0.002695139566718211	5.906589395259835E-4	4.543530304046026E-5	4.1304820945872964E-5	0.16193348527724766	5.782674932422215E-5
114-115	0.0026972048077655046	5.947894216205707E-4	4.543530304046026E-5	4.543530304046026E-5	0.16272447259836115	5.98919903715158E-5
116-117	0.0027075310130019728	5.947894216205707E-4	4.9565785135047556E-5	4.750054408775391E-5	0.16354024281204213	6.40224724661031E-5
118-119	0.002719922459285735	5.98919903715158E-4	5.369626722963485E-5	4.9565785135047556E-5	0.16434981730258125	6.81529545606904E-5
120-121	0.0027261181824276156	5.98919903715158E-4	5.369626722963485E-5	4.9565785135047556E-5	0.16519037040882972	7.228343665527769E-5
122-123	0.002730248664522203	6.030503858097453E-4	5.369626722963485E-5	4.9565785135047556E-5	0.16589255236490957	7.434867770257133E-5
124-125	0.0027323139055694967	6.092461089516263E-4	5.5761508276928504E-5	5.16310261823412E-5	0.16668147444497577	7.434867770257133E-5
126-127	0.0027570967981370206	6.216375552353881E-4	5.782674932422215E-5	5.369626722963485E-5	0.16765833346034564	7.641391874986498E-5
128-129	0.0027860101727991316	6.360942425664436E-4	5.782674932422215E-5	5.369626722963485E-5	0.1685918224137224	7.847915979715864E-5
130-131	0.002806662583272068	6.443552067556183E-4	5.782674932422215E-5	5.369626722963485E-5	0.16967400872250427	8.054440084445228E-5
132-133	0.002829380234792298	6.443552067556183E-4	5.782674932422215E-5	5.369626722963485E-5	0.17060336719378638	8.260964189174593E-5
134-135	0.0028500326452652346	6.505509298974992E-4	5.782674932422215E-5	5.369626722963485E-5	0.17144185505898762	9.087060608092052E-5
136-137	0.002854163127359822	6.526161709447929E-4	5.782674932422215E-5	5.369626722963485E-5	0.17240425738702647	9.293584712821417E-5
138-139	0.0028582936094544093	6.69138099323142E-4	5.782674932422215E-5	5.5761508276928504E-5	0.17321383187756556	9.500108817550782E-5
>>END_MODULE
