##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.control.2d.R.3.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24234100
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	67-150
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.381518851535645	36.0	34.0	38.0	31.0	39.0
2	34.698139769993524	35.0	34.0	37.0	31.0	39.0
3	34.80861290495624	35.0	34.0	38.0	31.0	39.0
4	34.81957922926785	35.0	34.0	38.0	31.0	39.0
5	34.69834873174576	35.0	34.0	37.0	31.0	39.0
6	34.693120932900335	35.0	34.0	37.0	31.0	39.0
7	34.71129383802163	35.0	34.0	37.0	32.0	39.0
8	34.83972266351959	35.0	34.0	37.0	31.0	39.0
9	34.80322772456992	35.0	34.0	37.0	31.0	39.0
10-14	34.77996896109202	35.0	34.0	37.0	31.0	39.0
15-19	34.79197858389625	35.0	34.0	37.0	31.0	39.0
20-24	34.782691991862706	35.0	34.0	37.0	31.0	39.0
25-29	34.778047462047276	35.0	34.0	37.0	31.0	39.0
30-34	34.761011260991744	35.0	34.0	37.0	31.0	39.0
35-39	34.75289566354847	35.0	34.0	37.0	31.0	39.0
40-44	34.706353633929055	35.0	34.0	37.0	31.0	39.0
45-49	34.61232918078245	35.0	34.0	37.0	31.0	39.0
50-54	34.582721900132455	35.0	34.0	37.0	31.0	39.0
55-59	34.5715899084348	35.0	34.0	37.0	31.0	39.0
60-64	34.544717361073864	35.0	34.0	37.0	30.2	39.0
65-69	34.512704998731884	35.0	34.0	37.0	30.0	39.0
70-74	34.51248372403709	35.0	34.0	37.0	30.0	39.0
75-79	34.49477369381246	35.0	34.0	37.0	30.0	39.0
80-84	34.48199447105519	35.0	34.0	37.0	30.0	39.0
85-89	34.46204227246035	35.0	34.0	37.0	30.0	39.0
90-94	34.44008803331903	35.0	34.0	37.0	30.0	39.0
95-99	34.42318032825473	35.0	34.0	37.0	30.0	39.0
100-104	34.38291847910423	35.0	34.0	37.0	30.0	39.0
105-109	34.358702267561775	35.0	34.0	37.0	30.0	39.0
110-114	34.32697523420612	35.0	34.0	37.0	30.0	39.0
115-119	34.29020796701967	35.0	34.0	37.0	30.0	39.0
120-124	34.25002241012136	35.0	34.0	37.0	30.0	39.0
125-129	34.238045492257505	35.0	34.0	37.0	30.0	39.0
130-134	34.18525567438796	35.0	34.0	37.0	29.2	39.0
135-139	34.15372967167692	35.0	34.0	37.0	29.0	39.0
140-144	34.108048618327935	35.0	34.0	37.0	29.0	39.0
145-149	34.14525719265354	35.0	34.0	37.0	29.2	39.0
150	35.02112310194179	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	10.0
22	237.0
23	2779.0
24	19271.0
25	80170.0
26	209186.0
27	386470.0
28	573081.0
29	751804.0
30	938983.0
31	1176020.0
32	1502270.0
33	1971354.0
34	2725630.0
35	4276096.0
36	8620768.0
37	999961.0
38	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.662892370667784	10.907238147899035	15.548805195984173	38.88106428544902
2	22.181229775866555	13.2966101425645	37.10003737352392	27.42212270804502
3	20.917212630993014	18.387566441659015	28.808732311078106	31.886488616269865
4	25.812241781415924	26.26533940583074	22.47362814114063	25.448790671612702
5	25.24762035471213	31.602796129119685	24.601224705346695	18.54835881082149
6	18.658756870746306	36.59442062698055	26.214697191992187	18.532125310280954
7	15.735842603817218	24.38714204235176	40.98448210251991	18.892533251311107
8	18.33954538555919	24.113386795798345	31.849737687522122	25.69733013112034
9	19.514986998470583	23.078601895711127	32.79294127705818	24.613469828760103
10-14	20.18708028004272	29.350422030652794	27.13556359022089	23.326934099083598
15-19	20.349753449190906	28.050256038293785	27.95888452928954	23.64110598322577
20-24	20.50299638801022	28.211079049192477	27.66165864990323	23.624265912894074
25-29	20.48848042081127	28.38388613537768	27.557864958443805	23.569768485367245
30-34	20.470601383548193	28.092998317606032	27.588852875631325	23.84754742321445
35-39	20.572899327099485	28.098249337602056	27.618180630372624	23.710670704925835
40-44	20.625625468540466	28.153113072894026	27.506381253279322	23.714880205286182
45-49	20.728990106392363	27.93049406620481	27.471507674172752	23.869008153230077
50-54	20.847468625776447	27.888683614165238	27.555424856245224	23.70842290381309
55-59	20.851540967165807	27.92077813108209	27.48133969759759	23.746341204154515
60-64	20.80848833382859	27.828401586798634	27.472253958558046	23.89085612081473
65-69	20.916478758847244	27.733768942577992	27.558807413174037	23.790944885400727
70-74	21.02263160533042	27.667241873712893	27.54040549315549	23.76972102780119
75-79	21.037680025550813	27.571201028634906	27.535391098313696	23.855727847500585
80-84	21.07959992708445	27.565155563848016	27.517020010644195	23.838224498423344
85-89	21.071593374986797	27.55349387210064	27.573295765030874	23.801616987881694
90-94	21.07753103756068	27.430830034958888	27.52190122639308	23.96973770108735
95-99	21.17163346647215	27.335573429980542	27.63852935202354	23.85426375152377
100-104	21.222333334783094	27.395847297080795	27.494590858771055	23.88722850936506
105-109	21.202816069139853	27.23067552812644	27.594496607627867	23.972011795105843
110-114	21.3033263844132	27.20023766579468	27.631930838903823	23.8645051108883
115-119	21.338580700990025	27.252991906653484	27.572511611397488	23.835915780959002
120-124	21.305052234330887	27.151013280729032	27.559984331347227	23.983950153592858
125-129	21.326539178714913	27.080394580192298	27.64669600377111	23.94637023732168
130-134	21.409161686764218	27.192008822230047	27.528302035664083	23.87052745534165
135-139	21.394014633739733	27.04144213440059	27.637373707835273	23.927169524024407
140-144	21.4596668602499	26.99001562317112	27.73598455834619	23.814332958232786
145-149	21.073295021097582	27.068059620368224	27.875203957388113	23.983441401146084
150	21.022210538654214	25.709800654810877	28.554770708081122	24.713218098453783
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	1.0
7	1.0
8	0.0
9	0.5
10	1.0
11	4.5
12	9.0
13	16.0
14	35.5
15	74.5
16	172.5
17	364.0
18	653.0
19	1212.0
20	2336.0
21	3954.0
22	6208.5
23	9843.5
24	15731.5
25	24644.0
26	36610.5
27	51613.0
28	70200.0
29	98057.5
30	133190.0
31	169294.0
32	202291.0
33	238501.0
34	279540.0
35	327783.5
36	403503.5
37	505681.0
38	626778.0
39	775555.0
40	943668.5
41	1106454.0
42	1272961.0
43	1444648.5
44	1580308.5
45	1658272.5
46	1674813.5
47	1628500.5
48	1536275.5
49	1407712.0
50	1242194.0
51	1062693.5
52	884382.0
53	706294.5
54	544852.5
55	412625.0
56	308252.5
57	229858.0
58	172444.5
59	127699.5
60	90406.5
61	65143.5
62	47384.0
63	31028.0
64	20313.5
65	15151.0
66	13766.5
67	10198.5
68	5021.5
69	2569.0
70	1994.5
71	1250.0
72	574.0
73	245.0
74	118.5
75	76.5
76	46.0
77	37.0
78	19.0
79	2.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0015143950053849741
3	0.13065886498776516
4	1.1966609034377179E-4
5	1.0728683961855403E-4
6	6.189625362608886E-5
7	7.427550435130663E-5
8	1.0728683961855403E-4
9	1.1553967343536587E-4
10-14	2.2530236319896345E-4
15-19	2.8389748329832754E-4
20-24	3.672511048481272E-4
25-29	2.987525841685889E-4
30-34	2.541872815578049E-4
35-39	1.1223853990864113E-4
40-44	2.781204996265593E-4
45-49	1.9889329498516553E-4
50-54	7.510078773298782E-5
55-59	1.4029817488580141E-4
60-64	1.9971857836684673E-4
65-69	0.05365827579581294
70-74	1.7330952159540623E-4
75-79	1.4524990394524818E-4
80-84	1.0728689097296517E-4
85-89	9.738355798454724E-5
90-94	1.2709396068893015E-4
95-99	1.1636552053973992E-4
100-104	8.417958506248448E-5
105-109	1.1141460357425475E-4
110-114	6.024666138586798E-5
115-119	6.354819550442239E-5
120-124	1.0316349452951224E-4
125-129	1.1059256768756205E-4
130-134	4.704403626342491E-5
135-139	5.282405355163886E-5
140-144	5.862318721933272E-5
145-149	3.4505204415774085E-5
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
66-67	1.0
68-69	0.0
70-71	0.0
72-73	2.0
74-75	1.0
76-77	2.0
78-79	0.0
80-81	6.0
82-83	6.0
84-85	6.0
86-87	10.0
88-89	12.0
90-91	15.0
92-93	18.0
94-95	26.0
96-97	22.0
98-99	31.0
100-101	39.0
102-103	32.0
104-105	47.0
106-107	33.0
108-109	43.0
110-111	42.0
112-113	36.0
114-115	53.0
116-117	75.0
118-119	82.0
120-121	71.0
122-123	105.0
124-125	113.0
126-127	146.0
128-129	180.0
130-131	206.0
132-133	287.0
134-135	435.0
136-137	832.0
138-139	2469.0
140-141	5294.0
142-143	8980.0
144-145	74408.0
146-147	776059.0
148-149	1395700.0
150-151	2.1968175E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.05609404446314
#Duplication Level	Percentage of total
1	39.85754048987988
2	10.217616578071821
3	6.2630144156065635
4	4.438970610728974
5	3.2878727686173335
6	2.600947826658832
7	2.1997833355519836
8	1.8144659645962666
9	1.5910861309679643
>10	17.979475995710157
>50	4.4463604537124075
>100	4.4590380965861645
>500	0.47703706865401296
>1k	0.34406289404208734
>5k	0.022727370615428073
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	8.252833816811847E-6	0.0	0.0	2.5171143141276137E-4	2.8884918358841467E-5
2	4.1264169084059235E-6	8.66547550765244E-5	0.0	1.2379250725217771E-5	0.002224138713630793	7.014908744290071E-5
3	4.1264169084059235E-6	9.903400580174217E-5	0.0	1.2379250725217771E-5	0.0034868222876030057	1.2791892416058365E-4
4	4.539058599246516E-5	1.1553967343536587E-4	0.0	1.2379250725217771E-5	0.0052364230567671175	1.4029817488580141E-4
5	4.9517002900871086E-5	1.1966609034377179E-4	0.0	1.2379250725217771E-5	0.006606393470357884	1.568038425194251E-4
6	9.078117198493032E-5	1.2379250725217772E-4	0.0	1.2379250725217771E-5	0.007720526035627484	1.6918309324464288E-4
7	9.490758889333625E-5	1.5267742561101918E-4	0.0	1.2379250725217771E-5	0.00904097944631738	1.6918309324464288E-4
8	1.1141325652695995E-4	1.568038425194251E-4	0.0	1.2379250725217771E-5	0.011454933337734844	1.8568876087826658E-4
9	1.1966609034377179E-4	1.568038425194251E-4	0.0	1.2379250725217771E-5	0.015226478392017859	1.9806801160348434E-4
10-11	1.5061421715681622E-4	1.7330951015304878E-4	0.0	1.2379250725217771E-5	0.020493849575598022	2.9091239204261766E-4
12-13	1.8775196933246954E-4	1.7743592706145471E-4	0.0	1.2379250725217771E-5	0.024857535456237284	3.693143133023302E-4
14-15	2.0219442851189027E-4	2.001312200576873E-4	0.0	1.2379250725217771E-5	0.02968544323907222	4.0438885702378055E-4
16-17	2.2488972150812287E-4	2.2901613841652877E-4	0.0	1.2379250725217771E-5	0.034597942568529466	4.167681077489983E-4
18-19	2.7853314131739986E-4	2.558378483211673E-4	0.0	1.2379250725217771E-5	0.038693411350122345	4.167681077489983E-4
20-21	3.4661902030609757E-4	3.136076850388502E-4	0.0	1.4442459179420732E-5	0.042722857461180735	4.1883131620320125E-4
22-23	4.2502094156581015E-4	3.218605188556621E-4	0.0	1.6505667633623694E-5	0.047067974465732174	4.5184265147044865E-4
24-25	4.807275698292901E-4	3.363029780350828E-4	0.0	1.8568876087826656E-5	0.05181748032730739	4.559690683788546E-4
26-27	5.42623823455379E-4	3.3836618648928576E-4	0.0	2.063208454202962E-5	0.05647620501689768	4.8485398673769604E-4
28-29	5.632559079974086E-4	3.3836618648928576E-4	0.0	2.063208454202962E-5	0.06105446457677405	5.219917389133494E-4
30-31	5.96267243264656E-4	3.3836618648928576E-4	0.0	3.713775217565331E-5	0.06537275987142085	5.611926995432057E-4
32-33	6.313417869861064E-4	3.3836618648928576E-4	0.0	4.745379444666813E-5	0.06967248628997982	6.49910663073933E-4
34-35	6.953012490663982E-4	3.3836618648928576E-4	0.0	6.395946208029182E-5	0.0735657606430608	6.643531222533537E-4
36-37	7.159333336084278E-4	3.404293949434887E-4	0.0	7.221229589710366E-5	0.07730016794516817	6.849852067953834E-4
38-39	7.510078773298782E-4	3.466190203060976E-4	0.0	7.427550435130663E-5	0.08166385382580743	7.159333336084278E-4
40-41	7.922720464139374E-4	3.5074543721450355E-4	0.0	7.427550435130663E-5	0.08526415257839161	7.324390012420515E-4
42-43	8.005248802307492E-4	3.5899827103131536E-4	0.0	7.427550435130663E-5	0.08884175603797954	7.427550435130663E-4
44-45	8.170305478643728E-4	3.693143133023302E-4	0.0	8.046512971391552E-5	0.09325908533842808	7.489446688756752E-4
46-47	8.582947169484322E-4	3.755039386649391E-4	0.0	8.252833816811847E-5	0.09757738063307489	7.551342942382841E-4
48-49	8.974956775782885E-4	3.8375677248175095E-4	0.0	8.459154662232144E-5	0.10169348149920979	7.695767534177049E-4
50-51	9.22254179028724E-4	3.9200960629856275E-4	0.0	8.66547550765244E-5	0.10600352396003979	7.860824210513285E-4
52-53	9.862136411090158E-4	3.961360232069687E-4	0.0	9.284438043913329E-5	0.1101258144515373	7.984616717765463E-4
54-55	0.001033667435555684	3.961360232069687E-4	0.0	9.903400580174217E-5	0.1132680809272884	8.108409225017641E-4
56-57	0.0010728683961855401	4.0438885702378055E-4	0.0	1.0109721425594514E-4	0.11791855278306189	8.232201732269819E-4
58-59	0.0011161957737238024	4.2502094156581015E-4	0.0	1.0522363116435105E-4	0.1224472953400374	8.294097985895908E-4
60-61	0.0011512703174452528	4.33273775382622E-4	0.0	1.0728683961855402E-4	0.12608473184479718	8.314730070437936E-4
62-63	0.0011822184442582973	4.3740019229102794E-4	0.0	1.1141325652695995E-4	0.12976549572709528	8.397258408606055E-4
64-65	0.0012069769457087327	4.3740019229102794E-4	0.0	1.1141325652695995E-4	0.1349152640287859	8.438522577690114E-4
66-67	0.0012358618640675742	4.518426514704487E-4	0.0	1.1347646498116292E-4	0.13881060159032108	8.459154662232144E-4
68-69	0.0013060109515104748	4.704115275582753E-4	0.0	1.1760288188956882E-4	0.14178781138973595	8.768635930362589E-4
70-71	0.0013823496643159845	4.8485398673769604E-4	0.0	1.2791892416058365E-4	0.14477121081451344	8.789268014904618E-4
72-73	0.001409171374220623	4.86917195191899E-4	0.0	1.3617175797739548E-4	0.14791554049871874	8.933692606698826E-4
74-75	0.0014318666672168555	4.86917195191899E-4	0.0	1.3617175797739548E-4	0.15125793819452754	8.954324691240854E-4
76-77	0.0014710676278467119	4.992964459171168E-4	0.0	1.4029817488580141E-4	0.15375442042411314	8.954324691240854E-4
78-79	0.001516458213839177	5.034228628255227E-4	0.0	1.671198847904399E-4	0.15639120082858454	8.995588860324914E-4
80-81	0.0015370902983812065	5.075492797339287E-4	0.0	1.733095101530488E-4	0.15931889362509852	9.057485113951003E-4
82-83	0.0015762912590110629	5.178653220049435E-4	0.0	1.7537271860725175E-4	0.16227340813151717	9.078117198493032E-4
84-85	0.0016670724309959931	5.199285304591465E-4	0.0	1.7743592706145471E-4	0.16476370073574015	9.078117198493032E-4
86-87	0.0017599168114351266	5.199285304591465E-4	0.0	1.8156234396986065E-4	0.1669692705732831	9.078117198493032E-4
88-89	0.0018114970227902005	5.199285304591465E-4	0.0	1.8156234396986065E-4	0.16944099430141824	9.119381367577092E-4
90-91	0.0018527611918742598	5.302445727301612E-4	0.0	1.8156234396986065E-4	0.17235424463875282	9.119381367577092E-4
92-93	0.001898151777866725	5.343709896385671E-4	0.0	1.8568876087826658E-4	0.17524273647463698	9.18127762120318E-4
94-95	0.0019662376568554225	5.40560615001176E-4	0.0	1.8568876087826658E-4	0.1783850029503881	9.22254179028724E-4
96-97	0.0020446395781151356	5.467502403637849E-4	0.0	1.8775196933246954E-4	0.18097432956041282	9.24317387482927E-4
98-99	0.0020982829979244123	5.611926995432057E-4	0.0	1.9187838624087547E-4	0.18310975031051288	9.24317387482927E-4
100-101	0.00216636887691311	5.653191164516116E-4	0.0	1.939415946950784E-4	0.1853689635678651	9.24317387482927E-4
102-103	0.002220012296722387	5.653191164516116E-4	0.0	1.939415946950784E-4	0.18736614935153356	9.24317387482927E-4
104-105	0.0022654028827148523	5.653191164516116E-4	0.0	1.939415946950784E-4	0.18919827845886583	9.263805959371299E-4
106-107	0.002325235927886738	5.818247840852353E-4	0.0	2.001312200576873E-4	0.19091074147585427	9.284438043913329E-4
108-109	0.002434585975959495	5.818247840852353E-4	0.0	2.063208454202962E-4	0.19270366962255664	9.284438043913329E-4
110-111	0.0025026718549481927	5.880144094478441E-4	0.0	2.063208454202962E-4	0.19438312130427784	9.284438043913329E-4
112-113	0.002558378483211673	5.96267243264656E-4	0.0	2.063208454202962E-4	0.196143038115713	9.305070128455358E-4
114-115	0.002609958694566747	6.024568686272649E-4	0.0	2.0838405387449914E-4	0.19825782678127102	9.325702212997388E-4
116-117	0.002657412489013415	6.168993278066856E-4	0.0	2.104472623287021E-4	0.2000631341786986	9.325702212997388E-4
118-119	0.0027420040356357367	6.375314123487153E-4	0.0	2.1251047078290507E-4	0.2015795923925378	9.325702212997388E-4
120-121	0.0028761125851589292	6.519738715281359E-4	0.0	2.1870009614551397E-4	0.20302796472738827	9.366966382081447E-4
122-123	0.0030411692614951656	6.519738715281359E-4	0.0	2.2488972150812287E-4	0.20445157856078833	9.366966382081447E-4
124-125	0.003154645726476329	6.561002884365419E-4	0.0	2.269529299623258E-4	0.20588138201955097	9.366966382081447E-4
126-127	0.003206225937831403	6.561002884365419E-4	0.0	2.269529299623258E-4	0.20736070248121447	9.366966382081447E-4
128-129	0.003257806149186477	6.561002884365419E-4	0.0	2.269529299623258E-4	0.20872035685253423	9.366966382081447E-4
130-131	0.003342397695808798	6.561002884365419E-4	0.0	2.331425553249347E-4	0.20991908096442616	9.449494720249566E-4
132-133	0.00346206378615257	6.664163307075567E-4	0.0	2.372689722333406E-4	0.21109304657486766	9.449494720249566E-4
134-135	0.0036869535076606928	6.684795391617597E-4	0.0	2.3933218068754357E-4	0.21214528288651116	9.490758889333625E-4
136-137	0.0038767686854473656	7.0355408288321E-4	0.0	2.3933218068754357E-4	0.21317688711361263	9.532023058417685E-4
138-139	0.0038829583108099743	7.138701251542248E-4	0.0	2.3933218068754357E-4	0.21439830651850078	9.532023058417685E-4
>>END_MODULE
