##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.control.2d.R.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24234100
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	63-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.84042877598095	36.0	35.0	38.0	33.0	39.0
2	35.211887010452216	35.0	34.0	37.0	32.0	39.0
3	35.334727759644466	35.0	34.0	37.0	33.0	39.0
4	35.415714881097294	36.0	35.0	37.0	33.0	39.0
5	35.328475990443216	35.0	34.0	37.0	33.0	39.0
6	35.284081273907425	35.0	34.0	37.0	33.0	39.0
7	35.3118048122274	35.0	34.0	37.0	33.0	39.0
8	35.456575734192725	36.0	34.0	38.0	33.0	39.0
9	35.429903318051835	36.0	34.0	38.0	33.0	39.0
10-14	35.39233779674096	35.4	34.2	37.6	33.0	39.0
15-19	35.36564720785999	35.8	34.0	38.0	33.0	39.0
20-24	35.33229779525545	35.8	34.0	37.6	32.8	39.0
25-29	35.32180119748619	36.0	34.0	37.8	32.2	39.0
30-34	35.30159965503155	36.0	34.0	37.6	32.0	39.0
35-39	35.277402742416676	35.4	34.0	37.0	32.0	39.0
40-44	35.255526840278776	35.6	34.0	37.0	32.0	39.0
45-49	35.22668249285098	35.0	34.0	37.0	32.0	39.0
50-54	35.20159221922828	35.0	34.0	37.0	32.0	39.0
55-59	35.17275534061508	35.0	34.0	37.0	32.0	39.0
60-64	35.13173201592033	35.0	34.0	37.0	32.0	39.0
65-69	35.09579347393503	35.0	34.0	37.0	32.0	39.0
70-74	35.07322116108055	35.0	34.0	37.0	32.0	39.0
75-79	35.02171398495441	35.0	34.0	37.0	32.0	39.0
80-84	34.95152130371762	35.0	34.0	37.0	31.4	39.0
85-89	34.930915153918114	35.0	34.0	37.0	31.0	39.0
90-94	34.87860315054553	35.0	34.0	37.0	31.0	39.0
95-99	34.81646865069582	35.0	34.0	37.0	31.0	39.0
100-104	34.75137937373174	35.0	34.0	37.0	31.0	39.0
105-109	34.69146943830601	35.0	34.0	37.0	30.8	39.0
110-114	34.628040100466215	35.0	34.0	37.0	30.0	39.0
115-119	34.56822293860877	35.0	34.0	37.0	30.0	39.0
120-124	34.493036241599086	35.0	34.0	37.0	30.0	39.0
125-129	34.413989064155274	35.0	34.0	37.0	30.0	39.0
130-134	34.34080196174799	35.0	34.0	37.0	30.0	39.0
135-139	34.25281374544404	35.0	34.0	37.0	29.0	39.0
140-144	34.158571901691715	35.0	34.0	37.0	29.0	39.0
145-149	34.127514077139665	35.0	34.0	37.0	29.0	39.0
150	34.95221722263949	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	209.0
22	1637.0
23	8896.0
24	30676.0
25	77492.0
26	158587.0
27	278306.0
28	413140.0
29	544666.0
30	694568.0
31	900117.0
32	1197120.0
33	1655308.0
34	2458721.0
35	4158792.0
36	1.0146749E7
37	1509106.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.77176788079607	18.42198802513813	16.70283195992424	32.103412134141564
2	24.075109040566804	24.823628688500914	34.18924985867022	16.91201241226206
3	19.567819725098108	29.053606282057103	30.871264045291554	20.50730994755324
4	23.359807048745363	33.92004241956582	22.478812912383788	20.24133761930503
5	25.234541410656885	35.050841582728474	22.58424699081047	17.13037001580418
6	18.74225162065024	38.59298674182247	23.196359675003407	19.46840196252388
7	20.073066464197144	20.158322363941718	38.594005966798846	21.17460520506229
8	21.31311251500984	22.85053705316063	28.73999859701825	27.096351834811276
9	23.362200370552237	23.07268270742466	28.849835562286202	24.7152813597369
10-14	23.063840811615375	28.97837286285336	25.780900679463244	22.176885646068026
15-19	22.903106143022487	28.22371805203179	27.18127206854203	21.69190373640369
20-24	23.028605646767197	28.310937196198545	27.027380190148026	21.63307696688623
25-29	23.120718326655414	28.30392298455482	26.941095398632502	21.634263290157257
30-34	23.127301803056866	28.067196455137157	27.126620151989307	21.678881589816676
35-39	23.212607854221943	28.049537634985413	27.095000845915468	21.642853664877176
40-44	23.36949441794409	27.819524783833728	27.187478199623488	21.62350259859869
45-49	23.368223288671746	27.638715694001426	27.274052677838252	21.71900833948857
50-54	23.43803502864175	27.614896592940497	27.234687710578793	21.71238066783896
55-59	23.61518769007308	27.50687832434462	27.129087525429046	21.748846460153253
60-64	23.533246516821283	27.492437969513006	27.17238976768091	21.8019257459848
65-69	23.61890482457168	27.415579339876718	27.139016945603263	21.826498889948343
70-74	23.66618648080704	27.385408667689692	27.06169193422855	21.88671291727472
75-79	23.607210722495875	27.444702897716468	27.13795008729503	21.810136292492626
80-84	23.699380158934265	27.48793258007889	27.018863509249268	21.79382375173758
85-89	23.73308801668861	27.401885226327316	27.0808908791871	21.784135877796977
90-94	23.65945237909467	27.352913247329646	27.1350611418122	21.852573231763486
95-99	23.74013244321488	27.480354845180372	27.12537513933806	21.65413757226669
100-104	23.886571872658845	27.4580463379385	26.928285567946524	21.727096221456133
105-109	23.67005053665301	27.58877443612839	27.037009082550295	21.7041659446683
110-114	23.686546797416273	27.59118428920855	26.98541871443697	21.736850198938207
115-119	23.77527710402247	27.576178690279114	27.026343463309804	21.62220074238861
120-124	23.737758137859373	27.47778825717232	27.224764337019288	21.559689267949018
125-129	23.709150100344328	27.557644573063282	27.18108621450372	21.552119112088665
130-134	23.756478622947437	27.475626648426104	27.263735604354505	21.504159124271954
135-139	23.6725922091603	27.49113315112961	27.351552054580214	21.48472258512988
140-144	23.69878456052959	27.600086501398312	27.341387650634363	21.359741287437735
145-149	23.403851971216085	27.58028343240586	27.422037675660597	21.59382692071745
150	23.53004843100348	26.434220597910734	27.846208917563935	22.18952205352185
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	3.5
2	11.0
3	21.0
4	29.5
5	33.5
6	24.0
7	12.5
8	10.0
9	11.0
10	10.5
11	8.0
12	13.0
13	18.0
14	24.5
15	41.0
16	77.5
17	135.0
18	229.0
19	415.0
20	628.5
21	942.5
22	1511.0
23	2486.0
24	4218.5
25	7017.5
26	10931.5
27	17176.0
28	28419.5
29	44136.5
30	59782.0
31	77462.0
32	101818.0
33	134372.5
34	181683.0
35	251636.5
36	349395.0
37	471665.0
38	617729.0
39	801227.0
40	1012958.5
41	1215418.0
42	1405602.5
43	1577590.5
44	1695489.5
45	1751818.5
46	1752180.5
47	1697459.0
48	1593276.5
49	1447803.5
50	1262230.0
51	1061956.0
52	870427.0
53	692845.5
54	541442.5
55	413871.5
56	310179.5
57	228442.0
58	164562.0
59	118336.0
60	82246.0
61	54548.5
62	36597.5
63	25289.0
64	17114.5
65	11190.5
66	7962.5
67	6054.0
68	5281.5
69	3808.0
70	1843.0
71	1101.5
72	592.0
73	423.5
74	377.5
75	261.5
76	179.5
77	64.5
78	14.0
79	0.0
80	0.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	8.252833816811849E-7
15-19	8.252833816811849E-7
20-24	0.027740250308449663
25-29	0.0
30-34	8.252833816811849E-7
35-39	0.0
40-44	8.252833816811849E-7
45-49	0.0
50-54	8.252833816811849E-7
55-59	0.0
60-64	0.0
65-69	8.25283463412312E-7
70-74	1.6505675125646358E-6
75-79	6.123613203804114E-4
80-84	0.0526690365848723
85-89	1.6505856434668657E-6
90-94	0.0
95-99	8.253096318272275E-7
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	3.2131064346049414E-4
145-149	0.02926210690911897
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62-63	2.0
64-65	0.0
66-67	1.0
68-69	1.0
70-71	7.0
72-73	8.0
74-75	10.0
76-77	21.0
78-79	32.0
80-81	44.0
82-83	50.0
84-85	62.0
86-87	76.0
88-89	98.0
90-91	92.0
92-93	91.0
94-95	113.0
96-97	117.0
98-99	160.0
100-101	190.0
102-103	145.0
104-105	187.0
106-107	168.0
108-109	181.0
110-111	204.0
112-113	188.0
114-115	212.0
116-117	240.0
118-119	261.0
120-121	296.0
122-123	339.0
124-125	393.0
126-127	466.0
128-129	492.0
130-131	619.0
132-133	810.0
134-135	1126.0
136-137	1709.0
138-139	3539.0
140-141	6934.0
142-143	11601.0
144-145	73469.0
146-147	845604.0
148-149	1127893.0
150-151	2.2155849E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.328918230444366
#Duplication Level	Percentage of total
1	28.96588182137854
2	10.68353810934927
3	6.8576285320495325
4	5.203601817267053
5	3.9897022244699722
6	3.1005632537547485
7	2.5402862634268186
8	2.182632434898362
9	1.8887016205147322
>10	21.181075250544108
>50	5.255294822681053
>100	6.155123369859461
>500	0.9734534379896252
>1k	0.8865100904721399
>5k	0.0900819808347478
>10k+	0.04592497050994368
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	8.252833816811847E-6	8.252833816811847E-6	0.0	0.0	0.0011760288188956882	0.0
2	2.4758501450435543E-5	1.6505667633623694E-5	0.0	4.1264169084059235E-6	0.005162147552415811	0.0
3	4.539058599246516E-5	1.6505667633623694E-5	0.0	4.1264169084059235E-6	0.006688921808526003	0.0
4	9.903400580174217E-5	2.4758501450435543E-5	0.0	4.1264169084059235E-6	0.008046512971391551	0.0
5	1.031604227101481E-4	3.301133526724739E-5	0.0	4.1264169084059235E-6	0.009701206151662327	0.0
6	1.031604227101481E-4	3.301133526724739E-5	0.0	4.1264169084059235E-6	0.011401289917925567	0.0
7	1.1553967343536587E-4	4.9517002900871086E-5	0.0	4.1264169084059235E-6	0.013683198468274043	0.0
8	1.6918309324464288E-4	4.9517002900871086E-5	0.0	4.1264169084059235E-6	0.019707767154546692	0.0
9	1.8156234396986065E-4	4.9517002900871086E-5	0.0	4.1264169084059235E-6	0.026132598280934716	0.0
10-11	2.1251047078290507E-4	5.1580211355074044E-5	0.0	4.1264169084059235E-6	0.03237586706335288	0.0
12-13	2.5790105677537026E-4	5.5706628263479976E-5	0.0	4.1264169084059235E-6	0.038419004625713356	0.0
14-15	3.0535485122203836E-4	5.7769836717682934E-5	0.0	4.1264169084059235E-6	0.04674817715533071	0.0
16-17	3.507454372145035E-4	6.189625362608886E-5	0.0	4.1264169084059235E-6	0.05270259675416046	0.0
18-19	4.2502094156581015E-4	7.221229589710366E-5	0.0	4.1264169084059235E-6	0.06038185862070389	0.0
20-21	4.7247473601247825E-4	7.633871280550959E-5	0.0	4.1264169084059235E-6	0.06639198484779711	0.0
22-23	5.42623823455379E-4	8.66547550765244E-5	4.1264169084059235E-6	4.1264169084059235E-6	0.07489240367911332	0.0
24-25	6.065832855356708E-4	8.871796353072736E-5	4.1264169084059235E-6	4.1264169084059235E-6	0.08029594662067087	4.1264169084059235E-6
26-27	6.416578292571212E-4	9.284438043913329E-5	4.1264169084059235E-6	4.1264169084059235E-6	0.08466375891821853	4.1264169084059235E-6
28-29	7.716399618719078E-4	9.903400580174218E-5	4.1264169084059235E-6	4.1264169084059235E-6	0.089161553348381	4.1264169084059235E-6
30-31	9.655815565669862E-4	1.0728683961855402E-4	6.189625362608885E-6	4.1264169084059235E-6	0.09514485786556959	4.1264169084059235E-6
32-33	0.001130638232903223	1.2172929879797475E-4	8.252833816811847E-6	4.1264169084059235E-6	0.10247750071180692	4.1264169084059235E-6
34-35	0.001244114697884386	1.2379250725217772E-4	8.252833816811847E-6	4.1264169084059235E-6	0.10592718524723427	4.1264169084059235E-6
36-37	0.0013101373684188808	1.3204534106898955E-4	8.252833816811847E-6	6.189625362608885E-6	0.10933147919666916	4.1264169084059235E-6
38-39	0.0013761600389533757	1.4236138334000438E-4	8.252833816811847E-6	8.252833816811847E-6	0.11331553472173508	4.1264169084059235E-6
40-41	0.001417424208037435	1.4855100870261325E-4	8.252833816811847E-6	8.252833816811847E-6	0.11616482559698937	4.1264169084059235E-6
42-43	0.0014359930841252614	1.5886705097362808E-4	8.252833816811847E-6	8.252833816811847E-6	0.11916060427249206	4.1264169084059235E-6
44-45	0.0014690044193925089	1.7949913551565768E-4	8.252833816811847E-6	8.252833816811847E-6	0.12165296008516924	4.1264169084059235E-6
46-47	0.00151852142229338	2.1251047078290507E-4	8.252833816811847E-6	8.252833816811847E-6	0.12451669341960296	4.1264169084059235E-6
48-49	0.0015618487998316421	2.228265130539199E-4	8.252833816811847E-6	1.4442459179420732E-5	0.1269223944772036	4.1264169084059235E-6
50-51	0.0016196186365493252	2.3107934687073174E-4	8.252833816811847E-6	1.6505667633623694E-5	0.1290062350159486	4.1264169084059235E-6
52-53	0.001681514890175414	2.3107934687073174E-4	1.237925072521777E-5	1.6505667633623694E-5	0.131094201971602	4.1264169084059235E-6
54-55	0.0017289686846220821	2.372689722333406E-4	1.6505667633623694E-5	1.6505667633623694E-5	0.13308726133836207	4.1264169084059235E-6
56-57	0.001790864938248171	2.3933218068754357E-4	1.8568876087826656E-5	1.6505667633623694E-5	0.13505349899521749	4.1264169084059235E-6
58-59	0.0018630772341452747	2.4758501450435543E-4	2.063208454202962E-5	1.8568876087826656E-5	0.1368546799757367	4.1264169084059235E-6
60-61	0.0019373527384965812	2.620274736837762E-4	2.063208454202962E-5	2.063208454202962E-5	0.1383484428965796	4.1264169084059235E-6
62-63	0.0019848065329432494	2.7853314131739986E-4	2.063208454202962E-5	2.063208454202962E-5	0.1405065589396759	4.1264169084059235E-6
64-65	0.002013691451302091	2.9503880895102353E-4	2.063208454202962E-5	2.063208454202962E-5	0.14214887286922145	4.1264169084059235E-6
66-67	0.002106535831741224	3.156708934930532E-4	2.063208454202962E-5	2.063208454202962E-5	0.14339505077556006	4.1264169084059235E-6
68-69	0.0021374839585542684	3.2805014421827093E-4	2.063208454202962E-5	2.063208454202962E-5	0.14469693531016214	4.1264169084059235E-6
70-71	0.002160179251550501	3.3217656112667686E-4	2.063208454202962E-5	2.063208454202962E-5	0.14604008401384827	4.1264169084059235E-6
72-73	0.0022035066290887633	3.5899827103131536E-4	2.063208454202962E-5	2.063208454202962E-5	0.14695821177596857	4.1264169084059235E-6
74-75	0.002246834006627026	3.631246879397213E-4	2.063208454202962E-5	2.063208454202962E-5	0.1479506150424402	4.1264169084059235E-6
76-77	0.002275718924985867	3.878831893901569E-4	2.063208454202962E-5	2.063208454202962E-5	0.1493474071659356	8.252833816811847E-6
78-79	0.0023293623447951444	4.126416908405924E-4	2.8884918358841467E-5	2.063208454202962E-5	0.15111145039427915	8.252833816811847E-6
80-81	0.002339678387066159	4.2502094156581015E-4	2.8884918358841467E-5	2.063208454202962E-5	0.15261759256584728	8.252833816811847E-6
82-83	0.002347931220882971	4.456530261078398E-4	3.507454372145035E-5	2.063208454202962E-5	0.154495112259172	1.237925072521777E-5
84-85	0.002376816139241812	4.456530261078398E-4	3.713775217565331E-5	2.4758501450435543E-5	0.15600538084764856	1.6505667633623694E-5
86-87	0.002391258598421233	4.5184265147044865E-4	3.713775217565331E-5	2.8884918358841467E-5	0.15794067037769094	1.6505667633623694E-5
88-89	0.0024057010576006535	4.5803227683305754E-4	3.713775217565331E-5	2.8884918358841467E-5	0.1597996211949278	1.6505667633623694E-5
90-91	0.0024180803083258713	4.7453794446668127E-4	3.713775217565331E-5	3.094812681304443E-5	0.1607755187937658	1.6505667633623694E-5
92-93	0.002426333142142683	4.827907782834931E-4	4.126416908405924E-5	3.713775217565331E-5	0.16175966922642063	1.8568876087826656E-5
94-95	0.002434585975959495	4.951700290087109E-4	4.126416908405924E-5	3.713775217565331E-5	0.1627169979491708	2.063208454202962E-5
96-97	0.002436649184413698	4.992964459171168E-4	4.126416908405924E-5	3.920096062985628E-5	0.16359798795911545	2.063208454202962E-5
98-99	0.002440775601322104	5.116756966423346E-4	4.745379444666813E-5	4.126416908405924E-5	0.16450786288741898	2.063208454202962E-5
100-101	0.0024449020182305096	5.199285304591465E-4	4.9517002900871086E-5	4.126416908405924E-5	0.1653001349338329	2.063208454202962E-5
102-103	0.002446965226684713	5.302445727301612E-4	5.1580211355074044E-5	4.539058599246516E-5	0.16623270515513264	2.063208454202962E-5
104-105	0.0024614076858641334	5.323077811843641E-4	5.364341980927701E-5	4.539058599246516E-5	0.16719416029479123	2.6821709904638505E-5
106-107	0.002488229395768772	5.570662826347997E-4	5.7769836717682934E-5	4.539058599246516E-5	0.1680834031385527	3.094812681304443E-5
108-109	0.002496482229585584	5.673823249058146E-4	6.189625362608886E-5	4.539058599246516E-5	0.16881790534824898	3.301133526724739E-5
110-111	0.002506798271856599	5.96267243264656E-4	6.189625362608886E-5	4.539058599246516E-5	0.16970714819201044	3.713775217565331E-5
112-113	0.0025129878972192077	6.230889531692945E-4	6.602267053449479E-5	4.539058599246516E-5	0.17051386269760377	3.713775217565331E-5
114-115	0.002515051105673411	6.272153700777004E-4	7.427550435130663E-5	4.539058599246516E-5	0.17140929516672787	3.713775217565331E-5
116-117	0.0025212407310360196	6.292785785319034E-4	7.427550435130663E-5	4.539058599246516E-5	0.17229028517667255	3.713775217565331E-5
118-119	0.0025253671479444255	6.561002884365419E-4	7.427550435130663E-5	4.539058599246516E-5	0.17307636759772388	4.126416908405924E-5
120-121	0.0025253671479444255	6.622899137991508E-4	7.427550435130663E-5	4.539058599246516E-5	0.1739429151484891	4.539058599246516E-5
122-123	0.0025274303563986283	6.746691645243685E-4	7.427550435130663E-5	4.539058599246516E-5	0.17467741735818537	5.1580211355074044E-5
124-125	0.0025398096071238465	6.829219983411804E-4	8.046512971391552E-5	4.539058599246516E-5	0.17550889036522915	5.983304517188589E-5
126-127	0.002562504900120079	6.849852067953834E-4	8.66547550765244E-5	4.539058599246516E-5	0.1765116096739718	6.808587898869774E-5
128-129	0.0025790105677537026	6.870484152495863E-4	8.871796353072736E-5	4.539058599246516E-5	0.17747925443899298	7.427550435130663E-5
130-131	0.0025893266100247172	7.159333336084278E-4	9.078117198493032E-5	4.539058599246516E-5	0.17844483599556	7.427550435130663E-5
132-133	0.002609958694566747	7.241861674252396E-4	9.490758889333625E-5	4.539058599246516E-5	0.17931963638014203	7.427550435130663E-5
134-135	0.002638843612925588	7.283125843336455E-4	9.490758889333625E-5	4.9517002900871086E-5	0.180132540511098	7.427550435130663E-5
136-137	0.0026470964467424	7.345022096962544E-4	9.697079734753922E-5	5.364341980927701E-5	0.18102178335485947	7.427550435130663E-5
138-139	0.002649159655196603	7.448182519672693E-4	1.031604227101481E-4	5.364341980927701E-5	0.18176247518991834	7.840192125971256E-5
>>END_MODULE
