##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.control.2d.S.1.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20729914
Total Bases	3.1 Gbp
Sequences flagged as poor quality	0
Sequence length	71-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.454942649545	36.0	34.0	38.0	31.0	39.0
2	34.661058603523394	35.0	34.0	37.0	31.0	39.0
3	34.856494532490586	35.0	34.0	38.0	31.0	39.0
4	34.8824217022801	35.0	34.0	38.0	31.0	39.0
5	34.73632842856946	35.0	34.0	37.0	31.0	39.0
6	34.704524533965746	35.0	34.0	37.0	31.0	39.0
7	34.740559705168096	35.0	34.0	37.0	32.0	39.0
8	34.84800482047345	35.0	34.0	37.0	32.0	39.0
9	34.82593019922803	35.0	34.0	37.0	32.0	39.0
10-14	34.819535160637905	35.0	34.0	37.0	31.2	39.0
15-19	34.83082723835709	35.0	34.0	37.0	31.0	39.0
20-24	34.81825729716004	35.0	34.0	37.0	31.0	39.0
25-29	34.82265937041514	35.0	34.0	37.0	31.0	39.0
30-34	34.79754908775791	35.0	34.0	37.0	31.0	39.0
35-39	34.78782758095378	35.0	34.0	37.0	31.0	39.0
40-44	34.73959214688493	35.0	34.0	37.0	31.0	39.0
45-49	34.63538099579188	35.0	34.0	37.0	31.0	39.0
50-54	34.608466306227804	35.0	34.0	37.0	31.0	39.0
55-59	34.60119071405699	35.0	34.0	37.0	30.8	39.0
60-64	34.56898551532824	35.0	34.0	37.0	30.0	39.0
65-69	34.53820412376047	35.0	34.0	37.0	30.0	39.0
70-74	34.537133430980205	35.0	34.0	37.0	30.0	39.0
75-79	34.521193661812006	35.0	34.0	37.0	30.0	39.0
80-84	34.49806641143183	35.0	34.0	37.0	30.0	39.0
85-89	34.48909279746307	35.0	34.0	37.0	30.0	39.0
90-94	34.46603863976772	35.0	34.0	37.0	30.0	39.0
95-99	34.442242517958945	35.0	34.0	37.0	30.0	39.0
100-104	34.403887745441736	35.0	34.0	37.0	30.0	39.0
105-109	34.37365916450457	35.0	34.0	37.0	30.0	39.0
110-114	34.34730333066144	35.0	34.0	37.0	30.0	39.0
115-119	34.31436686232267	35.0	34.0	37.0	30.0	39.0
120-124	34.26742564188909	35.0	34.0	37.0	30.0	39.0
125-129	34.25095112786936	35.0	34.0	37.0	30.0	39.0
130-134	34.2096764474223	35.0	34.0	37.0	29.2	39.0
135-139	34.16838018948169	35.0	34.0	37.0	29.0	39.0
140-144	34.12602154018018	35.0	34.0	37.0	29.0	39.0
145-149	34.163274024412296	35.0	34.0	37.0	29.2	39.0
150	35.05537586535206	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	7.0
22	190.0
23	2451.0
24	16703.0
25	69586.0
26	178984.0
27	333195.0
28	492352.0
29	644145.0
30	804760.0
31	1005569.0
32	1278135.0
33	1672126.0
34	2292255.0
35	3529764.0
36	7178036.0
37	1231654.0
38	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.537375022395175	10.495364331950437	11.710820411507736	44.25644023414665
2	21.72580419599648	11.670693192257243	40.66222255818812	25.941280053558152
3	19.33496028509264	16.07875131710065	29.051424787032502	35.5348636107742
4	25.526731093780793	24.14104082101758	22.837526595058293	27.494701490143335
5	26.54411538050946	29.58577809354314	25.066919107439073	18.803187418508326
6	19.196339005257386	34.834510659369315	27.23015588364335	18.738994451729955
7	16.396612987497136	23.931524772609755	40.59278941698246	19.079072822910646
8	18.204219310279687	23.019741406137353	33.03253887468047	25.74350040890249
9	19.460712306685288	21.81976793575529	33.841149842620766	24.878369914938656
10-14	20.99861315065377	28.143283826081028	26.903632735633998	23.9544702876312
15-19	21.326465182589022	27.28655564113156	27.222876453258237	24.164102723021177
20-24	21.241603707892516	27.24911896307906	27.261441299460415	24.247836029568013
25-29	21.146872988378124	27.5349348118375	26.938847601839676	24.379344597944705
30-34	21.23713861396102	27.271316922553083	27.08156553328427	24.409978930201625
35-39	21.54725818833301	27.048147416622403	26.98647220654116	24.41812218850343
40-44	21.78022729414565	27.140637625506876	26.738532658164303	24.34060242218317
45-49	21.926920895678855	27.121437154347916	26.670274792376475	24.281367157596755
50-54	21.930868269994633	27.342457851658896	26.600083456301498	24.126590422044966
55-59	21.90255816618106	27.31284976805076	26.622978941586585	24.161613124181592
60-64	22.111840890959193	27.10286973183851	26.414360970892915	24.370928406309382
65-69	21.972961409900712	27.246920957123088	26.65060442715525	24.129513205820952
70-74	22.381644531333965	27.029022423792398	26.48321110908419	24.106121935789442
75-79	22.208961919242682	26.872170575294213	26.524283313871727	24.394584191591377
80-84	22.503482823299283	26.91381377875394	26.550401113322554	24.032302284624222
85-89	22.363034519625636	26.919174044235	26.520592588887794	24.19719884725157
90-94	22.283472311101086	26.606024097129477	26.602327975697097	24.508175616072336
95-99	22.62960740577526	26.682559977826713	26.75752448256788	23.930308133830145
100-104	22.526891714378372	26.564698365335765	26.76313298799454	24.145276932291328
105-109	22.647626160312573	26.49063616672232	26.619616494863347	24.24212117810176
110-114	22.60546051313606	26.560863873209	26.641470358305458	24.19220525534948
115-119	22.70008035404987	26.80570774734703	26.503880048485474	23.990331850117627
120-124	22.638776219835997	26.634635423098775	26.520847934213453	24.20574042285178
125-129	23.018441623741577	26.636551059345248	26.407179751383858	23.937827565529314
130-134	22.71499015365303	26.967628673802007	26.283558853475387	24.033822319069575
135-139	22.8045684935108	26.71643410192547	26.35847140636111	24.120525998202616
140-144	22.828095333275396	26.622045255279602	26.62274793744445	23.927111474000547
145-149	22.22303168494489	26.59982921047278	27.054574827447	24.122564277135332
150	21.98789963100072	25.1410173730673	27.82305143654149	25.048031559390495
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.0
9	1.0
10	2.0
11	2.5
12	6.5
13	12.0
14	24.0
15	52.0
16	105.5
17	244.0
18	517.5
19	893.0
20	1385.5
21	2196.0
22	3546.0
23	5621.5
24	8848.0
25	13274.0
26	18630.0
27	26811.0
28	40379.0
29	58432.0
30	79945.5
31	101987.5
32	123337.5
33	150550.5
34	181638.0
35	220794.5
36	278616.5
37	344750.0
38	413148.5
39	506354.0
40	631492.0
41	771535.5
42	919001.5
43	1059265.5
44	1178119.5
45	1270673.5
46	1335034.5
47	1352913.5
48	1308968.0
49	1262893.0
50	1192783.5
51	1059747.0
52	899743.0
53	760753.5
54	636852.0
55	530827.0
56	462199.0
57	384232.0
58	316550.0
59	263847.0
60	205259.0
61	144008.5
62	84155.0
63	46240.0
64	29113.0
65	18458.5
66	12282.0
67	5957.5
68	2207.0
69	947.0
70	684.0
71	477.0
72	272.5
73	138.0
74	79.0
75	49.0
76	23.0
77	19.0
78	10.0
79	2.0
80	1.0
81	1.0
82	1.5
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0014327121665820707
3	0.12226775277504769
4	1.398944539760271E-4
5	1.6883813410899825E-4
6	1.398944539760271E-4
7	1.1095077384305599E-4
8	1.591902407313412E-4
9	2.4602128113025457E-4
10-14	2.354085984148318E-4
15-19	3.0969737742279105E-4
20-24	3.8109178841745314E-4
25-29	2.961903266940712E-4
30-34	2.537395958323802E-4
35-39	1.408592433137928E-4
40-44	2.566339638456773E-4
45-49	1.813803954999524E-4
50-54	1.1288035251858739E-4
55-59	1.601550300691069E-4
60-64	1.9199307821537515E-4
65-69	0.049106812502936574
70-74	1.871691387496982E-4
75-79	1.601550625174452E-4
80-84	1.2156352340989774E-4
85-89	1.1288048865095225E-4
90-94	1.4568350905996578E-4
95-99	9.16553593700996E-5
100-104	8.393726841152888E-5
105-109	1.0805754635504722E-4
110-114	8.683229031994206E-5
115-119	5.113480777432006E-5
120-124	1.0227037729066591E-4
125-129	1.0516607401885457E-4
130-134	5.306648596788479E-5
135-139	6.56126692506544E-5
140-144	5.887855332189951E-5
145-149	5.707676618394484E-5
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
70-71	1.0
72-73	1.0
74-75	1.0
76-77	2.0
78-79	1.0
80-81	5.0
82-83	5.0
84-85	7.0
86-87	4.0
88-89	6.0
90-91	12.0
92-93	13.0
94-95	17.0
96-97	17.0
98-99	23.0
100-101	37.0
102-103	22.0
104-105	25.0
106-107	37.0
108-109	31.0
110-111	28.0
112-113	38.0
114-115	35.0
116-117	48.0
118-119	64.0
120-121	65.0
122-123	87.0
124-125	92.0
126-127	128.0
128-129	151.0
130-131	184.0
132-133	238.0
134-135	357.0
136-137	701.0
138-139	1691.0
140-141	4610.0
142-143	7070.0
144-145	61261.0
146-147	681317.0
148-149	1221310.0
150-151	1.8750172E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.389537596702034
#Duplication Level	Percentage of total
1	32.95275151573251
2	8.672058680548112
3	5.136759451187728
4	3.5765127740410247
5	2.669066630171837
6	2.1800508596321997
7	1.8178092046143082
8	1.5321674659965396
9	1.2706128162105277
>10	16.278033576764347
>50	5.424338908998186
>100	10.42815154164549
>500	3.451501463404182
>1k	4.103981700599168
>5k	0.44079360872424567
>10k+	0.06540980172952535
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	9.647893377657041E-6	0.0	9.647893377657041E-6	1.9778181424196935E-4	9.647893377657041E-6
2	2.8943680132971126E-5	5.788736026594225E-5	0.0	9.647893377657041E-6	0.001649789767579354	1.4471840066485563E-5
3	3.376762682179965E-5	6.75352536435993E-5	0.0	9.647893377657041E-6	0.0038012699907968746	1.9295786755314083E-5
4	6.75352536435993E-5	6.75352536435993E-5	0.0	1.4471840066485563E-5	0.005947926267325566	1.9295786755314083E-5
5	7.235920033242781E-5	6.75352536435993E-5	0.0	1.9295786755314083E-5	0.006898243765024785	1.9295786755314083E-5
6	1.2059866722071302E-4	6.75352536435993E-5	0.0	1.9295786755314083E-5	0.008523913799159996	1.9295786755314083E-5
7	1.3024656059837007E-4	8.200709371008486E-5	0.0	1.9295786755314083E-5	0.009927682285609096	2.4119733444142606E-5
8	2.5084522781908307E-4	8.200709371008486E-5	0.0	1.9295786755314083E-5	0.012812402405528552	2.4119733444142606E-5
9	3.3285232152916795E-4	8.200709371008486E-5	0.0	1.9295786755314083E-5	0.015619939378426751	2.8943680132971126E-5
10-11	4.1244744189483857E-4	8.683104039891338E-5	0.0	1.9295786755314083E-5	0.02644246377481354	6.75352536435993E-5
12-13	4.413911220278097E-4	8.924301374332763E-5	0.0	1.9295786755314083E-5	0.03315498559231842	9.16549870877419E-5
14-15	4.848066422272664E-4	1.0853880049864172E-4	0.0	1.9295786755314083E-5	0.03849268260350718	9.406696043215616E-5
16-17	5.28222162426723E-4	1.1095077384305599E-4	0.0	1.9295786755314083E-5	0.04199004395290786	1.0130288046539894E-4
18-19	6.174651761700506E-4	1.157747205318845E-4	0.0	1.9295786755314083E-5	0.04514731706074612	1.0130288046539894E-4
20-21	9.382576309771473E-4	1.3265853394278432E-4	0.0	1.9295786755314083E-5	0.047556878431815976	1.0371485380981319E-4
22-23	0.0012807578458839722	1.3748248063161285E-4	0.0	1.9295786755314083E-5	0.050014679269774105	1.157747205318845E-4
24-25	0.001398944539760271	1.398944539760271E-4	0.0	1.9295786755314083E-5	0.052619610481741505	1.2059866722071302E-4
26-27	0.0014520079533373847	1.398944539760271E-4	0.0	1.9295786755314083E-5	0.05486274569204677	1.4230642732044138E-4
28-29	0.0015026593935700842	1.398944539760271E-4	0.0	2.1707760099728344E-5	0.05730848666328282	1.543662940425127E-4
30-31	0.0016522017409237684	1.398944539760271E-4	0.0	4.823946688828521E-5	0.06014737928965841	1.8813392086431232E-4
32-33	0.0017462687013559248	1.4471840066485563E-4	0.0	5.065144023269947E-5	0.06285602535543562	2.0984168096404066E-4
34-35	0.0018475715818213234	1.4471840066485563E-4	0.0	7.235920033242781E-5	0.06518357963279539	2.1707760099728344E-4
36-37	0.0018789272352987088	1.4713037400926988E-4	0.0	7.95951203656706E-5	0.06738571129624561	2.2672549437494048E-4
38-39	0.001898223022054023	1.5677826738692694E-4	0.0	9.16549870877419E-5	0.06982180437410401	2.2913746771935475E-4
40-41	0.0019175188088093372	1.6401418742016972E-4	0.0	9.16549870877419E-5	0.07200705222414333	2.31549441063769E-4
42-43	0.00192475472884258	1.6883813410899825E-4	0.0	9.647893377657042E-5	0.07439731780845787	2.387853610970118E-4
44-45	0.0019416385422534796	1.7366208079782675E-4	0.0	9.889090712098469E-5	0.07695159758019257	2.4119733444142604E-4
46-47	0.0019464624889423082	1.7366208079782675E-4	0.0	1.0612682715422746E-4	0.08057920549019161	2.4119733444142604E-4
48-49	0.001963346302353208	1.7366208079782675E-4	0.0	1.1095077384305599E-4	0.08292605555430668	2.4119733444142604E-4
50-51	0.0019826420891085224	1.7848602748665528E-4	0.0	1.1095077384305599E-4	0.08542003599243103	2.4119733444142604E-4
52-53	0.0020164097159303214	1.8089800083106954E-4	0.0	1.1095077384305599E-4	0.0874436816283946	2.4602128113025457E-4
54-55	0.0020381174760300503	1.833099741754838E-4	0.0	1.1095077384305599E-4	0.08897046075540882	2.4602128113025457E-4
56-57	0.002112888649706892	1.9054589420872657E-4	0.0	1.1095077384305599E-4	0.09091209929766231	2.5084522781908307E-4
58-59	0.0021490682498731062	1.9295786755314085E-4	0.0	1.157747205318845E-4	0.09276208285282805	2.5084522781908307E-4
60-61	0.002221427450205534	1.953698408975551E-4	0.0	1.157747205318845E-4	0.09473990099524773	2.5084522781908307E-4
62-63	0.002262430997060576	2.122536543084549E-4	0.0	1.157747205318845E-4	0.09694444463204237	2.5084522781908307E-4
64-65	0.002293786650537962	2.122536543084549E-4	0.0	1.157747205318845E-4	0.09946736875029968	2.532572011634973E-4
66-67	0.002305846517260033	2.2190154768611197E-4	0.0	1.2059866722071302E-4	0.10163090884023927	2.580811478523259E-4
68-69	0.002354085984148318	2.2913746771935475E-4	0.0	1.2301064056512728E-4	0.10327828663447422	2.6772904122998294E-4
70-71	0.0023830296642812893	2.4119733444142604E-4	0.0	1.2542261390954154E-4	0.1050317912558634	2.797889079520542E-4
72-73	0.002407149397725432	2.4119733444142604E-4	0.0	1.2542261390954154E-4	0.10648379920920077	2.797889079520542E-4
74-75	0.0024288571578251605	2.4119733444142604E-4	0.0	1.3265853394278432E-4	0.10790203953571637	2.8461285464088275E-4
76-77	0.0024409170245472318	2.4360930778584032E-4	0.0	1.3748248063161285E-4	0.10911526212795673	2.8461285464088275E-4
78-79	0.002465036757991374	2.484332544746688E-4	0.0	1.6160221407575544E-4	0.11022718183973171	2.87024827985297E-4
80-81	0.002537395958323802	2.5084522781908307E-4	0.0	1.6883813410899822E-4	0.11159235875267018	2.9426074801853976E-4
82-83	0.0025759875318344303	2.5084522781908307E-4	0.0	1.6883813410899822E-4	0.11307089841279612	3.0149666805178257E-4
84-85	0.0026338748921003723	2.5566917450791163E-4	0.0	1.6883813410899822E-4	0.1144529591391455	3.039086413961968E-4
86-87	0.002703822119088386	2.6531706788556863E-4	0.0	1.6883813410899822E-4	0.11573854093171829	3.039086413961968E-4
88-89	0.0027182939591548715	2.701410145743972E-4	0.0	1.712501074534125E-4	0.11703618259101316	3.039086413961968E-4
90-91	0.0027400017192546	2.701410145743972E-4	0.0	1.7366208079782675E-4	0.11870285617200341	3.039086413961968E-4
92-93	0.0028027130262093707	2.7255298791881144E-4	0.0	1.7366208079782675E-4	0.12032129028610539	3.039086413961968E-4
94-95	0.0028750722265417986	2.797889079520542E-4	0.0	1.7366208079782675E-4	0.12168887917238827	3.039086413961968E-4
96-97	0.002923311693430084	2.8220088129646845E-4	0.0	1.7366208079782675E-4	0.12291898557803954	3.039086413961968E-4
98-99	0.002990846947073683	2.8943680132971126E-4	0.0	1.8572194751989807E-4	0.12394648622276001	3.039086413961968E-4
100-101	0.0031114456142943957	3.039086413961968E-4	0.0	1.953698408975551E-4	0.12503428620109086	3.0632061474061107E-4
102-103	0.003150037187805024	3.039086413961968E-4	0.0	2.0260576093079788E-4	0.1260762586858778	3.087325880850253E-4
104-105	0.0031717449479047527	3.039086413961968E-4	0.0	2.1707760099728344E-4	0.1270724036771209	3.087325880850253E-4
106-107	0.0032658119083369087	3.039086413961968E-4	0.0	2.2190154768611195E-4	0.12809025642846372	3.087325880850253E-4
108-109	0.0033068154551919512	3.039086413961968E-4	0.0	2.2672549437494048E-4	0.12905745773957383	3.087325880850253E-4
110-111	0.003388822548902036	3.111445614294396E-4	0.0	2.2672549437494048E-4	0.1300463668107837	3.111445614294396E-4
112-113	0.003507009242778335	3.135565347738539E-4	0.0	2.2672549437494048E-4	0.13117517033596957	3.135565347738539E-4
114-115	0.0035576606830110343	3.183804814626824E-4	0.0	2.31549441063769E-4	0.13232085767456633	3.1596850811826813E-4
116-117	0.003627607909999048	3.183804814626824E-4	0.0	2.4119733444142604E-4	0.13324223149213257	3.183804814626824E-4
118-119	0.0037072030303647186	3.2079245480709663E-4	0.0	2.4119733444142604E-4	0.13404783058916694	3.232044281515109E-4
120-121	0.0038446855109963308	3.232044281515109E-4	0.0	2.4119733444142604E-4	0.13487996139298986	3.232044281515109E-4
122-123	0.004136534285670457	3.232044281515109E-4	0.0	2.4360930778584032E-4	0.1356831485166798	3.232044281515109E-4
124-125	0.004278840712990898	3.232044281515109E-4	0.0	2.4602128113025457E-4	0.13642121236007057	3.256164014959252E-4
126-127	0.004339140046601254	3.232044281515109E-4	0.0	2.4602128113025457E-4	0.1372075156703496	3.2802837484033944E-4
128-129	0.004440442927066654	3.232044281515109E-4	0.0	2.4602128113025457E-4	0.13797211122052894	3.2802837484033944E-4
130-131	0.0045731014610094385	3.232044281515109E-4	0.0	2.6531706788556863E-4	0.1386426398102761	3.304403481847537E-4
132-133	0.00464787263468628	3.232044281515109E-4	0.0	2.6772904122998294E-4	0.13931558037336766	3.352642948735822E-4
134-135	0.004758823408529336	3.232044281515109E-4	2.4119733444142603E-6	2.701410145743972E-4	0.1401959506440789	3.3767626821799645E-4
136-137	0.004901129835849777	3.376762682179965E-4	4.823946688828521E-6	2.7255298791881144E-4	0.14110044064823424	3.3767626821799645E-4
138-139	0.004901129835849777	3.473241615956535E-4	4.823946688828521E-6	2.7737693460763995E-4	0.1420097545990784	3.3767626821799645E-4
>>END_MODULE
