##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.control.2d.S.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20729914
Total Bases	3.1 Gbp
Sequences flagged as poor quality	0
Sequence length	68-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.9716458061524	37.0	35.0	38.0	33.0	39.0
2	35.2184149919773	35.0	34.0	37.0	33.0	39.0
3	35.36760837502751	35.0	34.0	37.0	33.0	39.0
4	35.44495269010764	36.0	35.0	37.0	33.0	39.0
5	35.37620870014222	36.0	34.0	37.0	33.0	39.0
6	35.303158855362355	35.0	34.0	37.0	33.0	39.0
7	35.35752367327718	35.0	34.0	37.0	33.0	39.0
8	35.45829485833853	35.0	34.0	38.0	33.0	39.0
9	35.47148849725088	36.0	34.0	38.0	33.0	39.0
10-14	35.43833716821015	35.8	34.4	37.8	33.0	39.0
15-19	35.40880988700677	36.0	34.0	38.0	33.0	39.0
20-24	35.38317700690895	36.0	34.0	38.0	33.0	39.0
25-29	35.371369606260785	36.0	34.0	38.0	33.0	39.0
30-34	35.35949141901891	36.0	34.0	38.0	32.8	39.0
35-39	35.329073058383166	36.0	34.0	38.0	32.0	39.0
40-44	35.316683330186514	36.0	34.0	38.0	32.0	39.0
45-49	35.286357608622986	36.0	34.0	38.0	32.0	39.0
50-54	35.26829181249859	36.0	34.0	38.0	32.0	39.0
55-59	35.238716764575095	36.0	34.0	38.0	32.0	39.0
60-64	35.197976142110385	36.0	34.0	38.0	32.0	39.0
65-69	35.161785592970396	36.0	34.0	38.0	32.0	39.0
70-74	35.14401674317076	36.0	34.0	38.0	32.0	39.0
75-79	35.084582984319056	35.4	34.0	38.0	32.0	39.0
80-84	35.01286754594308	35.4	34.0	38.0	31.6	39.0
85-89	34.99441223456315	35.0	34.0	37.6	31.2	39.0
90-94	34.946488001695045	35.0	34.0	37.2	31.0	39.0
95-99	34.881217255295624	35.0	34.0	37.2	31.0	39.0
100-104	34.821076448692565	35.0	34.0	37.0	31.0	39.0
105-109	34.76282504078336	35.0	34.0	37.0	30.8	39.0
110-114	34.6948086639173	35.0	34.0	37.0	30.0	39.0
115-119	34.63019518201689	35.0	34.0	37.0	30.0	39.0
120-124	34.55765694613568	35.0	34.0	37.0	30.0	39.0
125-129	34.477011293971586	35.0	34.0	37.0	30.0	39.0
130-134	34.398520095898405	35.0	34.0	37.0	30.0	39.0
135-139	34.31743626055658	35.0	34.0	37.0	29.2	39.0
140-144	34.230173125592444	35.0	34.0	37.0	29.0	39.0
145-149	34.196686728302765	35.0	34.0	37.0	29.0	39.0
150	35.01839612777842	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	12.0
21	154.0
22	1535.0
23	7776.0
24	27195.0
25	67235.0
26	135450.0
27	234332.0
28	343742.0
29	451642.0
30	579997.0
31	751756.0
32	998985.0
33	1376647.0
34	2042717.0
35	3414600.0
36	8515560.0
37	1780577.0
38	2.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.66091832315368	20.3946480434024	12.235043522129422	36.7093901113145
2	23.581482296549808	26.32938564048071	34.97315039512465	15.115981667844835
3	17.43661840565282	30.457444251818895	30.071711826686787	22.034225515841506
4	21.2877583573188	35.26412603544809	22.365090371334873	21.08302523589823
5	24.63135640601307	35.463427392897046	22.265495167997322	17.639721033092563
6	18.168777738296455	38.72081669031526	23.208460970942767	19.90194460044552
7	19.448122167800598	20.810293761951932	37.701439571818774	22.040144498428695
8	19.953966041537846	22.962618175839996	29.834243403035828	27.249172379586334
9	22.58564121394811	23.271133686324024	28.984987588467565	25.158237511260296
10-14	22.724917457206214	28.72340549722884	25.516946433226366	23.03473061233858
15-19	22.833228568466115	27.848931045723884	26.821400482620433	22.496439903189565
20-24	22.79494881875254	27.730532408633135	26.743400250100347	22.731118522513977
25-29	22.788758313227927	27.389567559228663	26.77554185704774	23.04613227049567
30-34	22.766841193841906	27.04563559694459	26.86943033145241	23.318092877761096
35-39	23.0737387526065	26.78714248404504	26.91967752495259	23.219441238395873
40-44	23.282735502740007	26.329268190203475	26.946290534020456	23.441705773036066
45-49	23.219687259677006	26.14542153913422	27.031668341701753	23.60322285948702
50-54	23.43819297502337	26.12154711419977	26.758711365215614	23.681548545561245
55-59	23.455451865357475	26.16503281200298	26.732229569307425	23.647285753332117
60-64	23.18230263762792	26.099039291721134	26.827422438896754	23.89123563175419
65-69	23.365282879275647	25.998386930099056	26.817595353435575	23.818734837189723
70-74	23.599232849487816	25.951285670405706	26.527214886031864	23.92226659407461
75-79	23.438953041537395	26.085753254610438	26.77544466339287	23.699849040459295
80-84	23.760601991891008	26.081797508355802	26.46601023207524	23.691590267677938
85-89	23.4997279571221	26.217777554888993	26.61509117560903	23.66740331237988
90-94	23.474048051913208	25.938582747010614	26.72263990825239	23.86472929282379
95-99	23.545930667846967	26.08996955959666	26.732426096258145	23.631673676298227
100-104	23.761272102345437	25.91213942196136	26.63478179036825	23.691806685324952
105-109	23.619257420866884	26.113727256841045	26.503696540011074	23.763318782280997
110-114	23.674150300842083	26.234955556163186	26.41790646745978	23.672987675534955
115-119	23.844295727584193	26.336985891506203	26.53893221903269	23.27978616187691
120-124	23.808400651624922	26.532266509487602	26.647154880023226	23.01217795886425
125-129	23.830511014398823	26.574446004211126	26.498245636380226	23.096797345009826
130-134	23.99034794074497	26.600760887162988	26.480636044867374	22.928255127224663
135-139	23.761613237733435	26.625544467136702	26.765768920257425	22.847073374872434
140-144	23.78942973796859	26.821874979239475	26.533006296830912	22.85568898596102
145-149	23.612611304734504	26.997787529261956	26.43183157752959	22.957769588473944
150	23.907235739345108	25.83495791396632	26.66803194455364	23.589774402134932
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	7.0
3	17.0
4	20.0
5	16.0
6	11.0
7	11.5
8	9.0
9	6.0
10	4.5
11	4.0
12	9.5
13	14.5
14	23.0
15	35.0
16	65.5
17	129.0
18	227.0
19	370.0
20	581.0
21	821.0
22	1207.5
23	1883.5
24	2959.5
25	4606.0
26	6899.5
27	10578.5
28	15998.0
29	22410.5
30	31038.5
31	44647.5
32	63568.0
33	86514.0
34	117627.0
35	166900.0
36	235284.0
37	316923.5
38	417418.0
39	539307.5
40	684439.5
41	841148.5
42	1003926.5
43	1164473.0
44	1286021.0
45	1370535.0
46	1398003.0
47	1363013.0
48	1321584.0
49	1271628.5
50	1172373.5
51	1040484.0
52	898080.0
53	763911.5
54	645870.0
55	553032.0
56	460818.5
57	362749.5
58	298242.0
59	243590.0
60	183122.0
61	126505.0
62	79068.0
63	47960.5
64	29388.0
65	14265.0
66	7347.5
67	4151.0
68	2522.5
69	1532.5
70	670.5
71	437.5
72	267.5
73	216.0
74	180.5
75	119.0
76	84.0
77	25.5
78	7.0
79	2.0
80	2.5
81	1.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	9.64789337765704E-7
15-19	9.64789337765704E-7
20-24	0.02543474131151726
25-29	0.0
30-34	0.0
35-39	0.0
40-44	9.64789337765704E-7
45-49	0.0
50-54	9.64789337765704E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	1.9295791409407536E-6
75-79	5.161626492762884E-4
80-84	0.049216902002436065
85-89	9.64793684707527E-7
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	2.5395366508198613E-4
145-149	0.025819520498500323
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
68-69	3.0
70-71	2.0
72-73	2.0
74-75	3.0
76-77	8.0
78-79	10.0
80-81	14.0
82-83	20.0
84-85	20.0
86-87	22.0
88-89	33.0
90-91	39.0
92-93	41.0
94-95	49.0
96-97	60.0
98-99	60.0
100-101	65.0
102-103	102.0
104-105	95.0
106-107	86.0
108-109	111.0
110-111	119.0
112-113	125.0
114-115	168.0
116-117	159.0
118-119	161.0
120-121	207.0
122-123	249.0
124-125	258.0
126-127	309.0
128-129	376.0
130-131	490.0
132-133	560.0
134-135	773.0
136-137	1341.0
138-139	3634.0
140-141	7323.0
142-143	8904.0
144-145	54902.0
146-147	702299.0
148-149	956683.0
150-151	1.8990029E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.15472209869681
#Duplication Level	Percentage of total
1	24.39798799224375
2	8.662435455705536
3	5.472507255597431
4	3.739373223494092
5	2.8172335962692285
6	2.3151922824820708
7	1.8593262914265167
8	1.605608154899834
9	1.430042740335629
>10	17.479128597513064
>50	6.216266584507757
>100	12.52040214194467
>500	3.8455570749259946
>1k	5.683815943291477
>5k	1.1448097278463747
>10k+	0.8103129375166527
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	31131	0.15017428436992067	No Hit
CTTAAACGTTTTACTTTGTGCTGCACTACTCAACCTCAATGGCCGCCTCA	26065	0.1257361704443154	No Hit
CAAATCTTAAACGTTTTACTTTGTGCTGCACTACTCAACCTCAATGGCCG	21290	0.1027018250051592	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.666199483509676E-4	4.823946688828521E-6	0.0	0.0	0.002141832329839863	0.0
2	0.0013265853394278432	9.647893377657041E-6	0.0	0.0	0.011133668957816227	0.0
3	0.0020935928629515783	9.647893377657041E-6	0.0	0.0	0.01649789767579354	0.0
4	0.005388348451421458	9.647893377657041E-6	0.0	0.0	0.024877093074288682	0.0
5	0.005566834478908113	9.647893377657041E-6	4.823946688828521E-6	0.0	0.029831286323715574	0.0
6	0.006628102750450388	9.647893377657041E-6	4.823946688828521E-6	0.0	0.041360518910015735	0.0
7	0.00887123796075565	9.647893377657041E-6	4.823946688828521E-6	4.823946688828521E-6	0.05544644324139502	0.0
8	0.01646895399566057	9.647893377657041E-6	4.823946688828521E-6	4.823946688828521E-6	0.07096507973935637	0.0
9	0.017110538905274763	9.647893377657041E-6	4.823946688828521E-6	4.823946688828521E-6	0.08130279749351589	0.0
10-11	0.017706296321345086	9.647893377657041E-6	4.823946688828521E-6	4.823946688828521E-6	0.1424125541475956	0.0
12-13	0.01842747635132495	9.647893377657041E-6	4.823946688828521E-6	4.823946688828521E-6	0.16939047600486912	0.0
14-15	0.021329080284655307	9.647893377657041E-6	4.823946688828521E-6	4.823946688828521E-6	0.18427958745993833	0.0
16-17	0.024963924114687598	9.647893377657041E-6	4.823946688828521E-6	4.823946688828521E-6	0.19422656553230275	0.0
18-19	0.03203100601382138	1.2059866722071301E-5	4.823946688828521E-6	4.823946688828521E-6	0.20366702920234014	0.0
20-21	0.03794998860101398	1.4471840066485563E-5	4.823946688828521E-6	4.823946688828521E-6	0.2088334761060755	0.0
22-23	0.04040778943897211	1.4471840066485563E-5	4.823946688828521E-6	4.823946688828521E-6	0.21431347954458468	0.0
24-25	0.04682846248180286	1.4471840066485563E-5	4.823946688828521E-6	4.823946688828521E-6	0.21961499695560724	0.0
26-27	0.052125155946136585	1.9295786755314083E-5	4.823946688828521E-6	4.823946688828521E-6	0.22451612679145702	0.0
28-29	0.06446963552284876	1.9295786755314083E-5	4.823946688828521E-6	4.823946688828521E-6	0.22926771427995313	0.0
30-31	0.09246299815812067	1.9295786755314083E-5	4.823946688828521E-6	4.823946688828521E-6	0.23445345697044379	0.0
32-33	0.11718813691171126	2.4119733444142606E-5	4.823946688828521E-6	4.823946688828521E-6	0.23853210389584828	0.0
34-35	0.13528517291485145	2.6531706788556867E-5	4.823946688828521E-6	4.823946688828521E-6	0.2443859632027417	0.0
36-37	0.1376079032455224	2.8943680132971126E-5	4.823946688828521E-6	4.823946688828521E-6	0.24708978532183007	0.0
38-39	0.1398775701626162	2.8943680132971126E-5	4.823946688828521E-6	4.823946688828521E-6	0.24907483938428304	0.0
40-41	0.14153218387688438	3.135565347738539E-5	4.823946688828521E-6	4.823946688828521E-6	0.2511853160606455	0.0
42-43	0.14192051158533509	3.376762682179965E-5	4.823946688828521E-6	4.823946688828521E-6	0.2528544016149802	0.0
44-45	0.1429045967098561	3.617960016621391E-5	4.823946688828521E-6	9.647893377657041E-6	0.25437635679530557	0.0
46-47	0.14379220290060055	4.823946688828521E-5	4.823946688828521E-6	9.647893377657041E-6	0.2559513753892081	0.0
48-49	0.14502954522628508	5.5475386921527986E-5	4.823946688828521E-6	9.647893377657041E-6	0.25721766139502555	0.0
50-51	0.14624517979186985	5.788736026594225E-5	4.823946688828521E-6	9.647893377657041E-6	0.2585104791076316	0.0
52-53	0.14759347289139743	5.788736026594225E-5	4.823946688828521E-6	9.647893377657041E-6	0.25987565602057006	0.0
54-55	0.14817958241409007	5.788736026594225E-5	4.823946688828521E-6	9.647893377657041E-6	0.26111299834625457	0.0
56-57	0.14907201255152336	6.512328029918503E-5	4.823946688828521E-6	9.647893377657041E-6	0.2622514497648182	0.0
58-59	0.14925532252569884	8.200709371008485E-5	4.823946688828521E-6	9.647893377657041E-6	0.26328618632957185	0.0
60-61	0.14955923116709505	8.924301374332763E-5	4.823946688828521E-6	9.647893377657041E-6	0.2640604297731288	0.0
62-63	0.14980042850153646	9.16549870877419E-5	4.823946688828521E-6	9.647893377657041E-6	0.2650903423911937	0.0
64-65	0.14987519967521332	1.0130288046539894E-4	4.823946688828521E-6	9.647893377657041E-6	0.26617090644949126	0.0
66-67	0.15000062228912286	1.2783458725395581E-4	4.823946688828521E-6	9.647893377657041E-6	0.2670898682937132	0.0
68-69	0.15006815754276645	1.3265853394278432E-4	4.823946688828521E-6	9.647893377657041E-6	0.26791717515084723	0.0
70-71	0.15022011186346457	1.350705072871986E-4	4.823946688828521E-6	9.647893377657041E-6	0.26882166515500255	0.0
72-73	0.15025870343697517	1.398944539760271E-4	4.823946688828521E-6	9.647893377657041E-6	0.2693667711308402	0.0
74-75	0.15026352738366403	1.4230642732044138E-4	4.823946688828521E-6	9.647893377657041E-6	0.2701651343078413	4.823946688828521E-6
76-77	0.1503286506639632	1.4954234735368413E-4	4.823946688828521E-6	9.647893377657041E-6	0.2711468074590179	4.823946688828521E-6
78-79	0.15039136197091796	1.712501074534125E-4	4.823946688828521E-6	9.647893377657041E-6	0.27231902650440326	7.2359200332427814E-6
80-81	0.1504637211712504	1.7366208079782675E-4	4.823946688828521E-6	9.647893377657041E-6	0.2731415094148485	1.2059866722071301E-5
82-83	0.15050472471810544	1.7366208079782675E-4	7.2359200332427814E-6	9.647893377657041E-6	0.2739857000853935	1.9295786755314083E-5
84-85	0.15055537615833814	1.7366208079782675E-4	9.647893377657041E-6	1.4471840066485563E-5	0.2748636583827603	1.9295786755314083E-5
86-87	0.150601203651882	1.7366208079782675E-4	1.4471840066485563E-5	1.4471840066485563E-5	0.27575126457350474	1.9295786755314083E-5
88-89	0.15065426706545912	1.7366208079782675E-4	1.4471840066485563E-5	1.4471840066485563E-5	0.2767401736447146	1.9295786755314083E-5
90-91	0.1506832107455921	2.0260576093079788E-4	1.4471840066485563E-5	1.4471840066485563E-5	0.27732628316740726	1.9295786755314083E-5
92-93	0.15070733047903623	2.0984168096404066E-4	1.9295786755314083E-5	1.4471840066485563E-5	0.2778713891432449	2.1707760099728344E-5
94-95	0.15079657349277956	2.146656276528692E-4	2.4119733444142606E-5	1.4471840066485563E-5	0.27850573813282586	2.4119733444142606E-5
96-97	0.15080863335950165	2.1707760099728344E-4	2.4119733444142606E-5	1.4471840066485563E-5	0.27914732304244005	2.4119733444142606E-5
98-99	0.15085687282638993	2.1707760099728344E-4	2.4119733444142606E-5	1.4471840066485563E-5	0.2796972529649665	2.6531706788556867E-5
100-101	0.15089305242655615	2.194895743416977E-4	2.4119733444142606E-5	1.4471840066485563E-5	0.2802713026209371	2.8943680132971126E-5
102-103	0.15090511229327822	2.2913746771935475E-4	2.4119733444142606E-5	1.4471840066485563E-5	0.2808188205701191	2.8943680132971126E-5
104-105	0.15092923202672234	2.31549441063769E-4	2.4119733444142606E-5	1.4471840066485563E-5	0.2813880462794009	3.376762682179965E-5
106-107	0.15095093978682209	2.31549441063769E-4	2.4119733444142606E-5	1.4471840066485563E-5	0.2820006875088821	3.376762682179965E-5
108-109	0.15097988346695507	2.3637338775259754E-4	2.4119733444142606E-5	1.4471840066485563E-5	0.2825144378312423	3.376762682179965E-5
110-111	0.15101606306712126	2.3637338775259754E-4	2.4119733444142606E-5	1.4471840066485563E-5	0.28308125156717967	3.376762682179965E-5
112-113	0.15106430253400954	2.387853610970118E-4	2.4119733444142606E-5	1.4471840066485563E-5	0.2837011287166942	3.376762682179965E-5
114-115	0.15109565818748694	2.4602128113025457E-4	2.4119733444142606E-5	1.4471840066485563E-5	0.28422452693243205	3.376762682179965E-5
116-117	0.15111495397424224	2.4602128113025457E-4	2.4119733444142606E-5	1.4471840066485563E-5	0.28481787237515793	3.8591573510628165E-5
118-119	0.1511414856810308	2.484332544746688E-4	2.4119733444142606E-5	1.4471840066485563E-5	0.28537503821771765	3.8591573510628165E-5
120-121	0.15114872160106405	2.5566917450791163E-4	2.4119733444142606E-5	1.4471840066485563E-5	0.2859804435271656	3.8591573510628165E-5
122-123	0.15115354554775287	2.580811478523259E-4	2.8943680132971126E-5	1.4471840066485563E-5	0.2864941938495259	3.8591573510628165E-5
124-125	0.15116560541447494	2.6049312119674013E-4	2.8943680132971126E-5	1.4471840066485563E-5	0.2870368878520191	4.341552019945669E-5
126-127	0.15119696106795233	2.629050945411544E-4	2.8943680132971126E-5	1.4471840066485563E-5	0.28766158894822236	5.065144023269947E-5
128-129	0.15121384488136325	2.7737693460763995E-4	2.8943680132971126E-5	1.4471840066485563E-5	0.2883248816179363	5.306341357711373E-5
130-131	0.15123072869477414	2.8943680132971126E-4	2.8943680132971126E-5	1.4471840066485563E-5	0.2890701813813603	5.5475386921527986E-5
132-133	0.15123796461480737	2.8943680132971126E-4	2.8943680132971126E-5	1.4471840066485563E-5	0.2897479458911407	6.029933361035651E-5
134-135	0.15125002448152944	2.8943680132971126E-4	2.8943680132971126E-5	1.4471840066485563E-5	0.29023034056002356	7.235920033242781E-5
136-137	0.15126449632159594	2.8943680132971126E-4	2.8943680132971126E-5	1.4471840066485563E-5	0.29080197824264975	7.235920033242781E-5
138-139	0.15126449632159594	2.9667272136295407E-4	2.8943680132971126E-5	1.4471840066485563E-5	0.2913904997386868	7.718314702125633E-5
>>END_MODULE
