##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.control.2d.S.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22339575
Total Bases	3.3 Gbp
Sequences flagged as poor quality	0
Sequence length	64-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.01035077883084	37.0	35.0	38.0	33.0	39.0
2	35.23004618485356	35.0	34.0	37.0	33.0	39.0
3	35.402526681908675	35.0	34.0	37.0	33.0	39.0
4	35.46592421744818	36.0	35.0	37.0	33.0	39.0
5	35.405553507620446	36.0	35.0	37.0	33.0	39.0
6	35.327769440555606	35.0	34.0	37.0	33.0	39.0
7	35.390941009397004	35.0	34.0	37.0	33.0	39.0
8	35.47919291213015	36.0	34.0	38.0	33.0	39.0
9	35.491411542072754	36.0	34.0	38.0	33.0	39.0
10-14	35.46718306861254	35.8	34.4	37.8	33.0	39.0
15-19	35.43772707403789	36.0	34.0	38.0	33.0	39.0
20-24	35.41078258650847	36.0	34.0	38.0	33.0	39.0
25-29	35.39895981906549	36.0	34.0	38.0	33.0	39.0
30-34	35.385501389350516	36.0	34.0	38.0	33.0	39.0
35-39	35.356276903208766	36.0	34.0	38.0	32.8	39.0
40-44	35.3439921932266	36.0	34.0	38.0	32.2	39.0
45-49	35.31234902185919	36.0	34.0	38.0	32.0	39.0
50-54	35.2939062538119	36.0	34.0	38.0	32.0	39.0
55-59	35.26420437273314	36.0	34.0	38.0	32.0	39.0
60-64	35.22313223953455	36.0	34.0	38.0	32.0	39.0
65-69	35.185808575378516	36.0	34.0	38.0	32.0	39.0
70-74	35.16751417826564	36.0	34.0	38.0	32.0	39.0
75-79	35.10815926029777	35.8	34.0	38.0	32.0	39.0
80-84	35.035476181439755	35.6	34.0	38.0	32.0	39.0
85-89	35.01462914882795	35.2	34.0	38.0	31.8	39.0
90-94	34.96763549013214	35.0	34.0	37.8	31.0	39.0
95-99	34.90100567940034	35.0	34.0	37.2	31.0	39.0
100-104	34.838928961360445	35.0	34.0	37.0	31.0	39.0
105-109	34.77904795198528	35.0	34.0	37.0	31.0	39.0
110-114	34.709683538410715	35.0	34.0	37.0	30.6	39.0
115-119	34.64303856093959	35.0	34.0	37.0	30.0	39.0
120-124	34.568851105405564	35.0	34.0	37.0	30.0	39.0
125-129	34.48734761069253	35.0	34.0	37.0	30.0	39.0
130-134	34.407043939745854	35.0	34.0	37.0	30.0	39.0
135-139	34.32227718948619	35.0	34.0	37.0	29.4	39.0
140-144	34.22978627327617	35.0	34.0	37.0	29.0	39.0
145-149	34.19378794158387	35.0	34.0	37.0	29.0	39.0
150	35.01424445283437	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	132.0
22	1219.0
23	6430.0
24	23489.0
25	60784.0
26	129331.0
27	238098.0
28	359786.0
29	475986.0
30	614476.0
31	803290.0
32	1074512.0
33	1501464.0
34	2250352.0
35	3804685.0
36	9130696.0
37	1864835.0
38	2.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.621491232487635	20.88761312603306	10.632538891183025	37.85835675029628
2	23.845860988850504	27.062139722890876	34.72489964558413	14.367099642674491
3	17.08175289816391	30.882722701752385	29.349049836444962	22.686474563638743
4	21.077482449867553	35.34631701811695	22.499067238297954	21.07713329371754
5	24.67707644393414	35.386147677384194	22.206554959080467	17.7302209196012
6	18.31165991295716	38.5006921573038	23.327534207790436	19.860113721948604
7	19.479345511273156	21.156002296373142	37.15946699970792	22.20518519264579
8	19.7958868957892	23.277099049556675	29.801104094415404	27.125909960238726
9	22.380340718209723	23.37857815110628	29.163647920786318	25.07743320989768
10-14	22.854264684981697	28.764548116962835	25.261892403951286	23.119294794104185
15-19	23.009422721868457	27.84339028690913	26.58658212241354	22.560604868808873
20-24	22.957999482312548	27.652502319452022	26.59310119903166	22.79639699920377
25-29	22.899133936075327	27.351045845769224	26.654080930366845	23.0957392877886
30-34	22.883970711170647	27.007727765635646	26.72565436003147	23.382647163162236
35-39	23.2080950510473	26.7031695992426	26.806606661048832	23.282128688661267
40-44	23.45175859433315	26.23106840662815	26.81593181607081	23.501241182967895
45-49	23.35882397046497	26.065163728495282	26.90393438550196	23.672077915537784
50-54	23.571849720255194	26.02592776295814	26.63880155856861	23.76342095821806
55-59	23.580067212558877	26.070730530907593	26.623437554205932	23.725764702327595
60-64	23.29002857037343	26.014374042478426	26.725091233830543	23.9705061533176
65-69	23.475487409499614	25.90426885871336	26.744784483971866	23.875459247815158
70-74	23.722326961634757	25.830013998927193	26.463754067950013	23.983904971488034
75-79	23.556482550319124	25.953538489341664	26.7357080028448	23.75427095749441
80-84	23.88599706537358	26.007961841974303	26.359616849029255	23.74642424362286
85-89	23.62202033646535	26.14856739931736	26.543120951309646	23.68629131290764
90-94	23.56233932990493	25.862713504527235	26.668305086600053	23.90664207896778
95-99	23.67719638490832	25.998302489806065	26.639805850371484	23.684695274914134
100-104	23.905726353451957	25.810903715264217	26.553240326462014	23.730129604821816
105-109	23.757663994995777	25.996390637639244	26.452240213286736	23.79370515407824
110-114	23.800587425212658	26.172346915631756	26.33618769100145	23.69087796815414
115-119	23.988730930576537	26.279826950226727	26.421080231081014	23.310361888115725
120-124	23.972448207542918	26.454057790771962	26.5287131169297	23.04478088475542
125-129	23.979381348224095	26.520049400886435	26.382555310857185	23.11801394003228
130-134	24.15108826629758	26.57550439946965	26.311653966439703	22.961753367793065
135-139	23.90856998472345	26.586873060068346	26.614005719663865	22.89055123554434
140-144	23.933618918050318	26.779313354445573	26.416966230992458	22.87010149651166
145-149	23.769564457633383	26.861083672054235	26.375197985097838	22.99415388521454
150	24.086118323083745	25.688626596052554	26.57592683953023	23.64932824133347
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	2.5
2	10.0
3	13.5
4	22.5
5	29.5
6	16.5
7	11.0
8	12.0
9	10.5
10	7.5
11	7.5
12	11.0
13	11.0
14	19.5
15	38.5
16	59.5
17	114.5
18	205.5
19	334.0
20	524.5
21	764.5
22	1138.5
23	1715.5
24	2720.0
25	4437.0
26	6857.5
27	10462.0
28	15523.0
29	21598.0
30	30233.0
31	43901.5
32	62494.5
33	85095.0
34	117135.5
35	168755.5
36	238981.0
37	321883.0
38	427190.5
39	557299.0
40	714578.0
41	883679.0
42	1060102.5
43	1234695.0
44	1368101.5
45	1468163.5
46	1506179.0
47	1469461.5
48	1426133.0
49	1383748.0
50	1286551.0
51	1144708.5
52	987805.5
53	843769.0
54	716398.0
55	612670.0
56	514912.5
57	408860.0
58	337621.0
59	278077.0
60	209357.5
61	145826.5
62	92030.5
63	55380.5
64	34129.0
65	16751.5
66	8338.5
67	4711.5
68	2780.5
69	1673.0
70	845.5
71	637.0
72	385.0
73	315.0
74	282.5
75	159.0
76	103.0
77	39.0
78	11.5
79	1.0
80	1.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	8.952721795289303E-7
20-24	0.025228770019125255
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.952721795289303E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	8.952724119675504E-7
75-79	5.684983276927854E-4
80-84	0.04845939448675907
85-89	8.952760989411158E-7
90-94	0.0
95-99	8.952829439682156E-7
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	2.562593175126238E-4
145-149	0.025899812059682416
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
64-65	2.0
66-67	0.0
68-69	1.0
70-71	2.0
72-73	4.0
74-75	5.0
76-77	8.0
78-79	11.0
80-81	13.0
82-83	17.0
84-85	19.0
86-87	29.0
88-89	30.0
90-91	23.0
92-93	30.0
94-95	55.0
96-97	35.0
98-99	77.0
100-101	72.0
102-103	90.0
104-105	99.0
106-107	95.0
108-109	107.0
110-111	112.0
112-113	114.0
114-115	135.0
116-117	160.0
118-119	157.0
120-121	219.0
122-123	211.0
124-125	227.0
126-127	292.0
128-129	417.0
130-131	459.0
132-133	602.0
134-135	886.0
136-137	1386.0
138-139	3879.0
140-141	8024.0
142-143	9442.0
144-145	58449.0
146-147	756094.0
148-149	1031133.0
150-151	2.0466353E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.75614810418625
#Duplication Level	Percentage of total
1	23.35807825550022
2	8.220320324514429
3	5.254476201018262
4	3.5529844755065967
5	2.894458533445775
6	2.233037929014716
7	1.8546878773079007
8	1.519528098319669
9	1.3601445225620814
>10	17.532655305333762
>50	6.105938581289632
>100	13.157224062415542
>500	4.222192208776862
>1k	6.429325765308606
>5k	1.409073816344003
>10k+	0.8958740433419538
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	33828	0.15142633644552325	No Hit
CTTAAACGTTTTACTTTGTGCTGCACTACTCAACCTCAATGGCCGCCTCA	30975	0.13865527880454306	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.7753437565396834E-4	1.3429082692933953E-5	0.0	0.0	0.00224265680971997	0.0
2	0.0010608975327417822	3.5810887181157204E-5	0.0	0.0	0.011710160108238407	0.0
3	0.0018532134116248855	3.5810887181157204E-5	0.0	0.0	0.017918872673271538	0.0
4	0.005237342250244242	3.5810887181157204E-5	0.0	0.0	0.026558249205725713	0.0
5	0.005523829347693499	3.5810887181157204E-5	0.0	0.0	0.031835878704048755	0.0
6	0.006777210399034002	3.5810887181157204E-5	0.0	0.0	0.04432492560847733	0.0
7	0.009275019779919716	4.4763608976446505E-5	0.0	0.0	0.05836279338349096	0.0
8	0.018518705033555922	4.4763608976446505E-5	0.0	0.0	0.07323774064636414	0.0
9	0.019185682807304972	4.923996987409116E-5	0.0	0.0	0.08279029480193782	0.0
10-11	0.019955616881699854	4.923996987409116E-5	0.0	0.0	0.15284086648917894	0.0
12-13	0.020660643723078888	4.923996987409116E-5	0.0	0.0	0.18700669104045176	0.0
14-15	0.023543420141162043	4.923996987409116E-5	0.0	0.0	0.20364756267744574	0.0
16-17	0.027220750618577122	5.1478150322913484E-5	0.0	0.0	0.21421848893723358	0.0
18-19	0.034022581002548166	5.819269166938046E-5	0.0	0.0	0.2235561777697203	0.0
20-21	0.0397903720191633	6.490723301584743E-5	0.0	0.0	0.22804596775005792	0.0
22-23	0.041880832558363355	7.609813525995906E-5	2.2381804488223252E-6	0.0	0.23327435727850687	0.0
24-25	0.04733527831214336	8.728903750407069E-5	4.4763608976446505E-6	0.0	0.23852289043099523	0.0
26-27	0.05198174092389851	8.952721795289301E-5	4.4763608976446505E-6	0.0	0.24356551098219192	0.0
28-29	0.06134852610221994	9.400357885053767E-5	4.4763608976446505E-6	4.4763608976446505E-6	0.24875585144301088	0.0
30-31	0.08485837353664964	9.624175929935999E-5	4.4763608976446505E-6	6.714541346466976E-6	0.2538947137535069	0.0
32-33	0.1112509973891625	9.847993974818232E-5	4.4763608976446505E-6	8.952721795289301E-6	0.25763695146393784	0.0
34-35	0.13137895416542167	9.847993974818232E-5	4.4763608976446505E-6	8.952721795289301E-6	0.26326821347317486	0.0
36-37	0.13409834341074078	9.847993974818232E-5	8.952721795289301E-6	8.952721795289301E-6	0.2656205411248871	0.0
38-39	0.1365760091675871	9.847993974818232E-5	8.952721795289301E-6	8.952721795289301E-6	0.2672409837698345	0.0
40-41	0.13843369894010965	1.0295630064582697E-4	8.952721795289301E-6	8.952721795289301E-6	0.26890171366286064	0.0
42-43	0.1387806169096771	1.0967084199229395E-4	8.952721795289301E-6	8.952721795289301E-6	0.2703005764433746	0.0
44-45	0.13967365090875722	1.1862356378758325E-4	8.952721795289301E-6	8.952721795289301E-6	0.27169943922388856	0.0
46-47	0.14015485970525401	1.3429082692933953E-4	1.3429082692933953E-5	8.952721795289301E-6	0.27327064189896183	0.0
48-49	0.14109713367420823	1.3429082692933953E-4	1.3429082692933953E-5	8.952721795289301E-6	0.2744210666496565	0.0
50-51	0.1422990365752258	1.3429082692933953E-4	1.3429082692933953E-5	1.3429082692933953E-5	0.2758109767083752	0.0
52-53	0.14373818660381857	1.3429082692933953E-4	1.3429082692933953E-5	1.3429082692933953E-5	0.27701511778984156	0.0
54-55	0.14440964073846524	1.4100536827580648E-4	1.3429082692933953E-5	1.3429082692933953E-5	0.27803348989405574	0.0
56-57	0.14528253111350597	1.4324354872462882E-4	1.3429082692933953E-5	1.3429082692933953E-5	0.2791413892162228	0.0
58-59	0.14545039464716764	1.4771990962227349E-4	1.3429082692933953E-5	1.3429082692933953E-5	0.28000756504991703	0.0
60-61	0.1456585454289081	1.4771990962227349E-4	1.3429082692933953E-5	1.3429082692933953E-5	0.28073945005668194	4.4763608976446505E-6
62-63	0.14591593618052268	1.4771990962227349E-4	1.3429082692933953E-5	1.3429082692933953E-5	0.28170410583012434	4.4763608976446505E-6
64-65	0.14594055616545973	1.5219627051991813E-4	1.3429082692933953E-5	1.3429082692933953E-5	0.2826732379644644	4.4763608976446505E-6
66-67	0.1460479888270032	1.7010171411049673E-4	1.3429082692933953E-5	1.3429082692933953E-5	0.28332902483596933	4.4763608976446505E-6
68-69	0.14610394333822377	1.7457807500814138E-4	1.3429082692933953E-5	1.3429082692933953E-5	0.28395123900074193	4.4763608976446505E-6
70-71	0.14626509233053897	1.7457807500814138E-4	1.3429082692933953E-5	1.3429082692933953E-5	0.28467193310526273	8.952721795289301E-6
72-73	0.1463367141049013	1.7905443590578602E-4	1.3429082692933953E-5	1.3429082692933953E-5	0.28518895278894074	1.7905443590578602E-5
74-75	0.14637476317253126	1.835307968034307E-4	1.3429082692933953E-5	1.3429082692933953E-5	0.28584473966044566	2.0143624039400927E-5
76-77	0.1463971449770195	1.947216990475423E-4	1.3429082692933953E-5	1.3429082692933953E-5	0.2866504846220217	2.461998493704558E-5
78-79	0.14644190858599593	2.0591260129165394E-4	1.3429082692933953E-5	1.7905443590578602E-5	0.28780762391406284	2.6858165385867906E-5
80-81	0.14649114855587003	2.0815078174047627E-4	1.3429082692933953E-5	1.7905443590578602E-5	0.28872303971763114	3.133452628351255E-5
82-83	0.14650457763856295	2.193416839845879E-4	1.3429082692933953E-5	1.7905443590578602E-5	0.28949073561157723	3.5810887181157204E-5
84-85	0.1465403885257441	2.2381804488223254E-4	1.5667263141756277E-5	1.7905443590578602E-5	0.2903949605129014	3.8049067629979526E-5
86-87	0.14658962849561818	2.2605622533105485E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.2913036617751233	4.0287248078801854E-5
88-89	0.14664558300683878	2.2829440577987718E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.29214521762388046	4.0287248078801854E-5
90-91	0.1466612502699805	2.3277076667752185E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.29272042999922787	4.0287248078801854E-5
92-93	0.1466881084353664	2.372471275751665E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.29319268607392934	4.0287248078801854E-5
94-95	0.14675749202927987	2.372471275751665E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.2937880420733161	4.0287248078801854E-5
96-97	0.14677092111197282	2.394853080239888E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.2943520635464193	4.0287248078801854E-5
98-99	0.14679106473601222	2.4396166892163347E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.29489817957593195	4.252542852762418E-5
100-101	0.14683582834498865	2.461998493704558E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.2954107228987123	4.4763608976446505E-5
102-103	0.1468514956081304	2.461998493704558E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.29593445712373667	4.4763608976446505E-5
104-105	0.14688283013441394	2.4843802981927815E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.296485049514147	4.923996987409116E-5
106-107	0.14689625921710686	2.506762102681004E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.29690582743852556	4.923996987409116E-5
108-109	0.14692983192383918	2.551525711657451E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.29739375077636887	4.923996987409116E-5
110-111	0.1469813100741621	2.6186711251221205E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.297845863227031	4.923996987409116E-5
112-113	0.14701488278089445	2.708198343075014E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.2984165992414807	5.371633077173581E-5
114-115	0.14702607368313855	2.7753437565396834E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.2989134753011192	5.371633077173581E-5
116-117	0.1470372645853827	2.797725561027907E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.2994103513607578	5.595451122055814E-5
118-119	0.14704621730717796	2.82010736551613E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.2999027510594987	5.819269166938046E-5
120-121	0.14705293184852442	2.82010736551613E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.3004488670890113	5.819269166938046E-5
122-123	0.14707531365301263	2.82010736551613E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.30089426499832694	5.819269166938046E-5
124-125	0.14710440999884733	2.82010736551613E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.30147619191502073	6.490723301584743E-5
126-127	0.14713798270557965	2.8648709744925763E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.3020693097339587	7.385995481113674E-5
128-129	0.14716260269051673	2.8872527789807997E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.3027609074926448	8.057449615760371E-5
130-131	0.14718498449500494	2.9320163879572464E-4	1.7905443590578602E-5	2.2381804488223252E-5	0.303463696153575	8.057449615760371E-5
132-133	0.14720512811904435	2.999161801421916E-4	1.7905443590578602E-5	2.461998493704558E-5	0.3041082921228358	8.057449615760371E-5
134-135	0.14722527174308375	2.999161801421916E-4	2.2381804488223252E-5	2.6858165385867906E-5	0.30463650270875786	8.057449615760371E-5
136-137	0.14724541536712316	3.0439254103983626E-4	2.2381804488223252E-5	2.6858165385867906E-5	0.305151284211987	8.057449615760371E-5
138-139	0.14724541536712316	3.0886890193748093E-4	2.2381804488223252E-5	2.6858165385867906E-5	0.3057376874895785	8.057449615760371E-5
>>END_MODULE
