##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.control.2d.S.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24116217
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	68-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.92347294768496	37.0	35.0	38.0	33.0	39.0
2	35.2263734399139	35.0	34.0	37.0	32.0	39.0
3	35.365487878965425	35.0	34.0	37.0	33.0	39.0
4	35.42810765054901	36.0	35.0	37.0	33.0	39.0
5	35.343378399688476	36.0	34.0	37.0	33.0	39.0
6	35.29889646456573	35.0	34.0	37.0	33.0	39.0
7	35.357598747763795	35.0	34.0	37.0	33.0	39.0
8	35.46543033677297	36.0	34.0	38.0	33.0	39.0
9	35.462874214475676	36.0	34.0	38.0	33.0	39.0
10-14	35.41644096999127	35.8	34.2	37.8	33.0	39.0
15-19	35.3873032242163	36.0	34.0	38.0	33.0	39.0
20-24	35.360485087690165	36.0	34.0	38.0	32.8	39.0
25-29	35.35284811046442	36.0	34.0	38.0	32.2	39.0
30-34	35.33990645381902	36.0	34.0	38.0	32.0	39.0
35-39	35.31210573366461	36.0	34.0	38.0	32.0	39.0
40-44	35.29758079386995	36.0	34.0	38.0	32.0	39.0
45-49	35.26999148332427	36.0	34.0	38.0	32.0	39.0
50-54	35.25090643362514	36.0	34.0	38.0	32.0	39.0
55-59	35.22040453525526	36.0	34.0	38.0	32.0	39.0
60-64	35.183834520978145	35.8	34.0	38.0	32.0	39.0
65-69	35.14606357311329	35.8	34.0	38.0	32.0	39.0
70-74	35.12844267559699	36.0	34.0	38.0	32.0	39.0
75-79	35.07417785718843	35.4	34.0	38.0	32.0	39.0
80-84	34.9955992272733	35.0	34.0	37.8	31.4	39.0
85-89	34.98567734947939	35.0	34.0	37.4	31.2	39.0
90-94	34.939479478822214	35.0	34.0	37.2	31.0	39.0
95-99	34.87176413982133	35.0	34.0	37.0	31.0	39.0
100-104	34.80845192899399	35.0	34.0	37.0	31.0	39.0
105-109	34.75615554897099	35.0	34.0	37.0	30.8	39.0
110-114	34.68657484255535	35.0	34.0	37.0	30.0	39.0
115-119	34.62595227588115	35.0	34.0	37.0	30.0	39.0
120-124	34.55585551714249	35.0	34.0	37.0	30.0	39.0
125-129	34.47762522415369	35.0	34.0	37.0	30.0	39.0
130-134	34.40096728522672	35.0	34.0	37.0	30.0	39.0
135-139	34.32013690362731	35.0	34.0	37.0	29.4	39.0
140-144	34.22952955034808	35.0	34.0	37.0	29.0	39.0
145-149	34.192137209245764	35.0	34.0	37.0	29.0	39.0
150	35.021078377208774	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	173.0
22	1762.0
23	9439.0
24	32475.0
25	79580.0
26	160108.0
27	277188.0
28	407693.0
29	535711.0
30	682866.0
31	881260.0
32	1165632.0
33	1603624.0
34	2370921.0
35	3946102.0
36	9877442.0
37	2084232.0
38	2.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.81668857930744	18.64673468479737	14.830775490202297	32.70580124569289
2	24.234555527510803	25.942290202480763	32.74146604336824	17.08168822664019
3	19.568807164075526	29.584548853578486	29.479781177951747	21.366862804394238
4	23.2617454055916	34.3939764681998	21.50698843023348	20.83728969597512
5	25.210405097947163	35.13153825079613	21.636001202012736	18.022055449243968
6	18.967427602762076	38.80868214115008	22.072130964819234	20.151759291268608
7	20.41347115096866	20.553124065851623	36.731552879956254	22.30185190322346
8	21.5054624860939	23.39242510547985	28.034935993485217	27.067176414941034
9	23.4636427429725	23.55439080681684	27.587249691773795	25.394716758436864
10-14	23.165427645637788	28.708364997710877	25.101739630224756	23.02446772642658
15-19	23.20550065054566	27.662674915600157	26.602453885739273	22.529370548114912
20-24	23.114981045124093	27.583325662362096	26.541250490206586	22.760442802307224
25-29	23.18725445205606	27.185721541649755	26.52986245728341	23.097161549010774
30-34	23.041310334867198	26.910373214837136	26.742161094337476	23.30615535595819
35-39	23.351161585583675	26.649119138378957	26.789314426885447	23.21040484915192
40-44	23.587761712378025	26.26155005986221	26.795441424332843	23.355246803426922
45-49	23.448526138643768	26.097326343491822	26.884219252830732	23.569928265033678
50-54	23.674540029844152	26.130752813600516	26.5758184758067	23.618888680748633
55-59	23.69402630603299	26.147508127000187	26.61638514863256	23.54208041833427
60-64	23.38726362751889	26.106825345839486	26.68385698041991	23.822054046221712
65-69	23.61818210226075	26.040836554430047	26.694006996984704	23.646974346324505
70-74	23.780450025413195	26.025651773810544	26.391344371778064	23.8025538289982
75-79	23.596282676271528	26.126499897387696	26.676367660788287	23.60084976555249
80-84	23.924646205190182	26.139739345343433	26.359992009823426	23.57562243964296
85-89	23.626241486366258	26.25819138031392	26.580672873454738	23.534894259865084
90-94	23.643580147432512	26.014284070919825	26.620800649686238	23.72133513196142
95-99	23.68225618985724	26.143782025175188	26.660476607620957	23.51348517734661
100-104	23.92431810860474	25.94814943902565	26.58707768292261	23.540454769447003
105-109	23.719393861787378	26.187298220425237	26.457724317847365	23.63558359994002
110-114	23.823597133473985	26.279915071068626	26.352439672369304	23.544048123088082
115-119	23.958645788826548	26.38759225373922	26.500950177094385	23.152811780339846
120-124	23.951183430308614	26.576428146558357	26.574781817433564	22.897606605699465
125-129	23.91697820153354	26.62682062648292	26.436089394656758	23.020111777326775
130-134	24.04711990389386	26.664043722141756	26.4493743975388	22.839461976425582
135-139	23.815775960941355	26.630218687872762	26.767318976900512	22.786686374285374
140-144	23.865522868977422	26.781931076234734	26.526104091160853	22.82644196362699
145-149	23.64375432230088	26.832672918054552	26.533348214501036	22.99022454514354
150	23.81901970574571	25.71907969084076	26.818165953458294	23.643734649955235
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	2.0
2	11.5
3	29.5
4	39.5
5	34.5
6	28.0
7	21.5
8	14.0
9	11.0
10	8.5
11	5.5
12	8.5
13	12.5
14	17.5
15	28.5
16	50.0
17	100.5
18	170.0
19	264.0
20	405.5
21	617.0
22	954.0
23	1523.5
24	2449.0
25	3908.0
26	6083.0
27	9486.5
28	14648.5
29	20790.0
30	29320.5
31	42352.0
32	60951.5
33	84691.0
34	117000.5
35	168736.0
36	242198.5
37	331411.0
38	445617.5
39	585621.5
40	753987.0
41	938976.5
42	1132222.5
43	1328545.5
44	1481697.0
45	1585826.5
46	1626187.5
47	1601263.5
48	1561539.0
49	1517324.5
50	1416812.0
51	1262038.5
52	1084476.0
53	918504.0
54	780904.0
55	670830.0
56	558901.0
57	442579.0
58	365828.5
59	302680.0
60	228214.5
61	154404.0
62	96618.5
63	59120.0
64	36281.5
65	17977.0
66	9716.0
67	5426.5
68	3126.5
69	1906.5
70	899.5
71	607.5
72	389.0
73	285.0
74	256.0
75	161.5
76	95.0
77	36.0
78	12.5
79	2.0
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	1.658634934326557E-6
20-24	0.03322992159176541
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	8.293174671632785E-7
50-54	8.293174671632785E-7
55-59	0.0
60-64	8.293174671632785E-7
65-69	0.0
70-74	1.6586352919657142E-6
75-79	7.47215669986701E-4
80-84	0.0645774253499335
85-89	2.4879631100753E-6
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	3.784899669655451E-4
145-149	0.03455546198170888
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
68-69	2.0
70-71	3.0
72-73	5.0
74-75	7.0
76-77	8.0
78-79	12.0
80-81	11.0
82-83	17.0
84-85	26.0
86-87	27.0
88-89	40.0
90-91	54.0
92-93	38.0
94-95	58.0
96-97	77.0
98-99	75.0
100-101	90.0
102-103	84.0
104-105	99.0
106-107	112.0
108-109	124.0
110-111	121.0
112-113	123.0
114-115	153.0
116-117	167.0
118-119	182.0
120-121	235.0
122-123	279.0
124-125	286.0
126-127	358.0
128-129	405.0
130-131	576.0
132-133	691.0
134-135	966.0
136-137	1587.0
138-139	4441.0
140-141	8513.0
142-143	10321.0
144-145	64161.0
146-147	799102.0
148-149	1103753.0
150-151	2.2118828E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.31139887794779
#Duplication Level	Percentage of total
1	24.841393248022104
2	8.3857845136892
3	5.2355268794727845
4	3.677297766655345
5	2.8409672225021625
6	2.2292347766999883
7	1.8019550984779353
8	1.5395527080074896
9	1.332197912305775
>10	17.219058956910256
>50	6.109227877522241
>100	12.672229586768182
>500	4.059100367316147
>1k	6.066297170485594
>5k	1.164415220724103
>10k+	0.8257606944406927
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	32303	0.1339472107088769	No Hit
GAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCCG	25205	0.10451473379925218	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.897792095667409E-4	1.2439762007449179E-5	0.0	0.0	0.0027782135149969833	0.0
2	0.001720833744363803	3.7319286022347537E-5	0.0	0.0	0.011316036839442935	0.0
3	0.0025086853381689174	3.7319286022347537E-5	0.0	0.0	0.0158855760835126	0.0
4	0.0057471700474415206	3.7319286022347537E-5	0.0	0.0	0.023934102102332218	0.0
5	0.005950352826896523	4.9759048029796715E-5	0.0	0.0	0.029407597385609857	0.0
6	0.0068294293420895985	4.9759048029796715E-5	0.0	0.0	0.03840154531699561	0.0
7	0.00873685951656514	5.390563536561311E-5	0.0	0.0	0.05110668891393704	0.0
8	0.015649220605371068	5.390563536561311E-5	0.0	0.0	0.06801232548206047	0.0
9	0.016296088229758424	7.049198470887867E-5	0.0	0.0	0.08019499907468904	0.0
10-11	0.017083939823563536	7.049198470887867E-5	2.0732936679081965E-6	0.0	0.13265762204743803	0.0
12-13	0.017969236219760338	7.049198470887867E-5	4.146587335816393E-6	0.0	0.15762215110272065	0.0
14-15	0.021340411723779065	7.049198470887867E-5	4.146587335816393E-6	0.0	0.16845096393020514	0.0
16-17	0.02506197385767428	7.049198470887867E-5	4.146587335816393E-6	0.0	0.17664254721211042	0.0
18-19	0.03211117232856214	7.049198470887867E-5	4.146587335816393E-6	0.0	0.18415823675827764	0.0
20-21	0.03801383940109678	7.463857204469506E-5	4.146587335816393E-6	0.0	0.18858679203292955	0.0
22-23	0.04033592830915396	8.293174671632786E-5	8.293174671632786E-6	0.0	0.19344037250950263	0.0
24-25	0.04632982030307656	8.707833405214425E-5	8.293174671632786E-6	0.0	0.19787722095882618	0.0
26-27	0.05176392300666394	9.122492138796064E-5	8.293174671632786E-6	0.0	0.20144950594863198	0.0
28-29	0.06486921228151166	9.329821505586884E-5	1.4513055675357375E-5	0.0	0.20496581200940428	0.0
30-31	0.09046402261183834	1.0366468339540982E-4	1.6586349343265572E-5	0.0	0.20890299668476198	0.0
32-33	0.11089011182806988	1.0781127073122622E-4	1.6586349343265572E-5	0.0	0.21180975440716926	0.0
34-35	0.12510668650891638	1.0988456439913441E-4	1.6586349343265572E-5	0.0	0.21493006137737108	0.0
36-37	0.12736243001960051	1.1403115173495081E-4	1.6586349343265572E-5	0.0	0.21694322952890993	0.0
38-39	0.1296409797606316	1.181777390707672E-4	1.6586349343265572E-5	0.0	0.2184795401368299	0.0
40-41	0.1312063164799023	1.243976200744918E-4	1.6586349343265572E-5	0.0	0.2199184059423582	0.0
42-43	0.1315152372364206	1.3061750107821638E-4	1.6586349343265572E-5	4.146587335816393E-6	0.22160606698803548	0.0
44-45	0.13254773748303889	1.3269079474612457E-4	1.6586349343265572E-5	4.146587335816393E-6	0.22292260846715717	2.0732936679081965E-6
46-47	0.1338393994381457	1.3269079474612457E-4	1.6586349343265572E-5	4.146587335816393E-6	0.22428476240697287	4.146587335816393E-6
48-49	0.1348470201607491	1.3269079474612457E-4	1.6586349343265572E-5	4.146587335816393E-6	0.22532140924092697	4.146587335816393E-6
50-51	0.135865007351692	1.3476408841403277E-4	1.6586349343265572E-5	4.146587335816393E-6	0.22648452698862348	4.146587335816393E-6
52-53	0.1369970256943699	1.3683738208194096E-4	1.6586349343265572E-5	4.146587335816393E-6	0.22754605334659245	4.146587335816393E-6
54-55	0.1375319354606902	1.4098396941775735E-4	1.6586349343265572E-5	4.146587335816393E-6	0.22853086783884885	6.2198810037245894E-6
56-57	0.13828868764947672	1.4098396941775735E-4	1.6586349343265572E-5	4.146587335816393E-6	0.22947006987041127	8.293174671632786E-6
58-59	0.1384110119758833	1.4305726308566554E-4	1.6586349343265572E-5	4.146587335816393E-6	0.23035121967927225	1.0366468339540982E-5
60-61	0.13870749297039414	1.5342373142520653E-4	1.6586349343265572E-5	4.146587335816393E-6	0.23111211845539456	1.6586349343265572E-5
62-63	0.13903714666359157	1.617169060968393E-4	1.6586349343265572E-5	4.146587335816393E-6	0.23207827330463976	2.0732936679081965E-5
64-65	0.13907861253694973	1.7830325544010488E-4	1.6586349343265572E-5	4.146587335816393E-6	0.23281221926307927	2.0732936679081965E-5
66-67	0.13915739769633023	1.9074301744755406E-4	1.6586349343265572E-5	4.146587335816393E-6	0.23338652160908985	2.0732936679081965E-5
68-69	0.13920715674436002	1.9696289845127867E-4	1.6586349343265572E-5	4.146587335816393E-6	0.23402302276513764	2.0732936679081965E-5
70-71	0.13929216178474427	1.9903619211918686E-4	1.6586349343265572E-5	8.293174671632786E-6	0.2348170942399465	2.0732936679081965E-5
72-73	0.1393274077770987	2.1562254146245243E-4	1.6586349343265572E-5	8.293174671632786E-6	0.23537273694294591	2.0732936679081965E-5
74-75	0.13933984753910617	2.1976912879826882E-4	2.0732936679081965E-5	8.293174671632786E-6	0.23598435857497882	2.280623034699016E-5
76-77	0.13937924011879643	2.280623034699016E-4	2.4879524014898358E-5	8.293174671632786E-6	0.23682611580414956	2.4879524014898358E-5
78-79	0.13943729234149785	2.32208890805718E-4	2.4879524014898358E-5	8.293174671632786E-6	0.2378275166457492	2.4879524014898358E-5
80-81	0.13949741785786718	2.363554781415344E-4	2.4879524014898358E-5	8.293174671632786E-6	0.23867342046225576	2.4879524014898358E-5
82-83	0.13952022408821418	2.4050206547735078E-4	2.902611135071475E-5	8.293174671632786E-6	0.23954213050910927	2.4879524014898358E-5
84-85	0.13956376325524023	2.4464865281316716E-4	3.3172698686531144E-5	8.293174671632786E-6	0.24033827527758603	2.4879524014898358E-5
86-87	0.13960730242226632	2.4879524014898355E-4	3.3172698686531144E-5	8.293174671632786E-6	0.24110124734737626	2.4879524014898358E-5
88-89	0.13962181547794167	2.5501512115270813E-4	3.3172698686531144E-5	8.293174671632786E-6	0.2418704392981702	2.4879524014898358E-5
90-91	0.13965291488296028	2.6952817682806553E-4	3.3172698686531144E-5	8.293174671632786E-6	0.2423535167227928	2.4879524014898358E-5
92-93	0.1396777944069752	2.6952817682806553E-4	3.3172698686531144E-5	8.293174671632786E-6	0.24288220660810939	2.4879524014898358E-5
94-95	0.13972133357400127	2.6952817682806553E-4	3.524599235443934E-5	8.293174671632786E-6	0.24359749292353772	2.4879524014898358E-5
96-97	0.13973584662967664	2.7160147049597373E-4	3.7319286022347537E-5	8.293174671632786E-6	0.2441759418568841	2.4879524014898358E-5
98-99	0.13975865286002362	2.736747641638819E-4	3.7319286022347537E-5	8.293174671632786E-6	0.2446092602334769	2.902611135071475E-5
100-101	0.13980011873338177	2.757480578317901E-4	3.7319286022347537E-5	8.293174671632786E-6	0.2450073326177153	3.3172698686531144E-5
102-103	0.1398125584953892	2.778213514996983E-4	3.7319286022347537E-5	8.293174671632786E-6	0.24549662992334162	3.3172698686531144E-5
104-105	0.13982292496372875	2.819679388355147E-4	3.7319286022347537E-5	8.293174671632786E-6	0.24601288004665078	3.3172698686531144E-5
106-107	0.13984158460673995	2.819679388355147E-4	3.7319286022347537E-5	8.293174671632786E-6	0.24653949663829944	3.9392579690255736E-5
108-109	0.1398768305990944	2.840412325034229E-4	3.7319286022347537E-5	8.293174671632786E-6	0.24704538029326906	4.146587335816393E-5
110-111	0.13992451635345626	2.902611135071475E-4	3.9392579690255736E-5	8.293174671632786E-6	0.2475844366469252	4.146587335816393E-5
112-113	0.13994110270279952	2.985542881787803E-4	4.146587335816393E-5	8.293174671632786E-6	0.24820435145362973	4.146587335816393E-5
114-115	0.13995561575847487	3.006275818466885E-4	4.146587335816393E-5	8.293174671632786E-6	0.24871230840226724	4.146587335816393E-5
116-117	0.1399597623458107	3.0477416918250487E-4	4.146587335816393E-5	8.293174671632786E-6	0.24922233864457266	4.146587335816393E-5
118-119	0.13997634869515396	3.1099405018622945E-4	4.146587335816393E-5	8.293174671632786E-6	0.24972822229954225	4.146587335816393E-5
120-121	0.13998878845716142	3.1514063752204584E-4	4.146587335816393E-5	8.293174671632786E-6	0.25031703770122815	4.146587335816393E-5
122-123	0.13999293504449722	3.192872248578622E-4	4.146587335816393E-5	8.293174671632786E-6	0.25083536111820526	4.561246069398032E-5
124-125	0.13999915492550097	3.234338121936786E-4	4.146587335816393E-5	8.293174671632786E-6	0.25138893052753675	4.561246069398032E-5
126-127	0.14000952139384049	3.234338121936786E-4	4.146587335816393E-5	8.293174671632786E-6	0.2520005521595696	4.561246069398032E-5
128-129	0.14001574127484423	3.234338121936786E-4	4.146587335816393E-5	8.293174671632786E-6	0.2526370533156175	4.561246069398032E-5
130-131	0.14002610774318378	3.2758039952949505E-4	4.146587335816393E-5	8.293174671632786E-6	0.2532859942336727	4.561246069398032E-5
132-133	0.1400385475051912	3.3172698686531144E-4	4.146587335816393E-5	8.293174671632786E-6	0.253889322691034	4.561246069398032E-5
134-135	0.14005098726719867	3.37946867869036E-4	4.146587335816393E-5	8.293174671632786E-6	0.2544138659890148	4.768575436188852E-5
136-137	0.14006342702920613	3.400201615369442E-4	4.146587335816393E-5	8.293174671632786E-6	0.25492596952498814	4.9759048029796715E-5
138-139	0.14006342702920613	3.400201615369442E-4	4.146587335816393E-5	8.293174671632786E-6	0.2555583240937001	4.9759048029796715E-5
>>END_MODULE
