##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.control.4d.R.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24095793
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	62-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.071634953039315	37.0	35.0	38.0	33.0	39.0
2	35.41663223119488	35.0	34.0	37.0	33.0	39.0
3	35.537351105232354	35.0	35.0	37.0	33.0	39.0
4	35.616675616361746	36.0	35.0	38.0	33.0	39.0
5	35.53096019707672	36.0	35.0	38.0	33.0	39.0
6	35.48014991662652	35.0	35.0	37.0	33.0	39.0
7	35.49723082365457	35.0	34.0	38.0	33.0	39.0
8	35.63757955590007	36.0	35.0	38.0	33.0	39.0
9	35.60101661729913	36.0	35.0	38.0	33.0	39.0
10-14	35.58057365449645	35.8	35.0	37.8	33.0	39.0
15-19	35.55719795567633	36.0	35.0	38.0	33.0	39.0
20-24	35.54136714238872	36.0	35.0	38.0	33.0	39.0
25-29	35.522848797713365	36.0	35.0	38.0	33.0	39.0
30-34	35.50438067757305	36.0	35.0	38.0	33.0	39.0
35-39	35.47749284698785	36.0	35.0	38.0	33.0	39.0
40-44	35.460090066344776	36.0	34.8	38.0	33.0	39.0
45-49	35.43030581313509	36.0	34.0	38.0	33.0	39.0
50-54	35.40603231443763	36.0	34.0	38.0	33.0	39.0
55-59	35.38286687638792	36.0	34.0	38.0	33.0	39.0
60-64	35.34111295439956	36.0	34.0	38.0	32.2	39.0
65-69	35.31392027821611	36.0	34.0	38.0	32.0	39.0
70-74	35.280434495949464	36.0	34.0	38.0	32.0	39.0
75-79	35.23539389751698	36.0	34.0	38.0	32.0	39.0
80-84	35.19494239815379	36.0	34.0	38.0	32.0	39.0
85-89	35.14533410802479	36.0	34.0	38.0	32.0	39.0
90-94	35.08922728036767	35.4	34.0	38.0	32.0	39.0
95-99	35.0242720451734	35.0	34.0	37.0	32.0	39.0
100-104	34.97820657990396	35.0	34.0	37.0	31.8	39.0
105-109	34.91391992582042	35.0	34.0	37.0	31.0	39.0
110-114	34.846201056800304	35.0	34.0	37.0	31.0	39.0
115-119	34.776132512580006	35.0	34.0	37.0	31.0	39.0
120-124	34.70076452130421	35.0	34.0	37.0	30.8	39.0
125-129	34.621314236238575	35.0	34.0	37.0	30.0	39.0
130-134	34.51609116951712	35.0	34.0	37.0	30.0	39.0
135-139	34.45381490533533	35.0	34.0	37.0	30.0	39.0
140-144	34.32561972041511	35.0	34.0	37.0	29.4	39.0
145-149	34.312836859737075	35.0	34.0	37.0	29.2	39.0
150	35.081840796896415	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	136.0
22	1270.0
23	7126.0
24	25613.0
25	65176.0
26	132012.0
27	232549.0
28	349758.0
29	463688.0
30	600603.0
31	787280.0
32	1058627.0
33	1493459.0
34	2269966.0
35	3941015.0
36	1.0849744E7
37	1817757.0
38	11.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.55518118868302	18.51786326351658	16.61055936195999	31.316396185840407
2	24.62794646351751	25.01868272191747	33.49820858769827	16.855162226866742
3	19.989755058071758	29.095410970703472	30.422725660035344	20.492108311189426
4	23.85339631694213	33.75712515458611	22.29702919509642	20.09244933337533
5	25.597559706791973	35.02459122221045	22.30398061603534	17.07386845496224
6	19.07998213630072	38.74919576209839	22.864920859836406	19.305901241764488
7	20.478346572781398	20.36772560255643	38.19913293577846	20.954794888883715
8	21.679174451739357	23.044167917611176	28.496173585156548	26.78048404549292
9	23.624725693817176	23.16890753502074	28.700371056474467	24.50599571468762
10-14	23.353485813892906	29.022876317040076	25.644440919624433	21.979196949442585
15-19	23.155421363389035	28.203265192392713	27.107152688438184	21.534160755780064
20-24	23.255037923010047	28.288099088500633	26.979670683591944	21.477192304897375
25-29	23.335789778738555	28.24576306743671	26.929705114913627	21.488742038911106
30-34	23.288944256783747	28.045385350048452	27.12420877785595	21.541461615311853
35-39	23.363907550168612	28.04648927719457	27.091286018268836	21.498317154367985
40-44	23.510542275989838	27.836066652797026	27.191029571012663	21.462361500200473
45-49	23.486867603817814	27.693243380701354	27.272555835784278	21.547333179696558
50-54	23.56468367735397	27.67780915116593	27.243383938432736	21.514123233047364
55-59	23.7154543948813	27.581208055696692	27.14226587188892	21.561071677533086
60-64	23.615795654278557	27.570295261512882	27.204950142331906	21.608958941876658
65-69	23.70037775567267	27.494970655948375	27.185613663972386	21.619037924406566
70-74	23.77932802714502	27.47209561514233	27.083469647052084	21.665106710660567
75-79	23.69037093661834	27.503795467809418	27.192115948330425	21.61371764724182
80-84	23.79111370300722	27.537106335027918	27.065989099427878	21.605790862536985
85-89	23.8170107747165	27.472079538155075	27.11998672148451	21.590922965643916
90-94	23.747399190935766	27.406286458514735	27.18833262453363	21.657981726015873
95-99	23.809041154962713	27.528254750577812	27.17933309233096	21.483371002128514
100-104	23.970564639533958	27.490659094057673	26.993630677931506	21.545145588476867
105-109	23.750650178397613	27.610067310760876	27.115393838814793	21.523888672026718
110-114	23.78566331379843	27.605084720254915	27.052957724587777	21.55629424135888
115-119	23.864268984907756	27.59500838015279	27.089149378962873	21.451573255976577
120-124	23.825716511621327	27.501053278492016	27.273337423808318	21.399892786078343
125-129	23.7990349924608	27.5893050380015	27.22504835539361	21.38661161414409
130-134	23.861739745094695	27.522126668832225	27.26292596721486	21.353207618858228
135-139	23.769653348469422	27.530806762284637	27.35495351813599	21.34458637110995
140-144	23.781850331925135	27.672830725769547	27.315903924453572	21.229415017851743
145-149	23.539953111409165	27.824258767901	27.224387761648504	21.411400359041323
150	23.666131531854344	26.56479047841305	27.637752491611355	22.131325498121246
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	3.5
10	6.0
11	6.0
12	9.0
13	16.5
14	25.0
15	35.5
16	62.0
17	119.0
18	198.5
19	333.5
20	544.5
21	874.5
22	1321.5
23	2231.0
24	3907.0
25	6474.5
26	10300.0
27	16486.5
28	26957.5
29	41018.5
30	56077.5
31	74068.0
32	97587.0
33	128958.5
34	174942.5
35	244129.0
36	342062.5
37	466108.5
38	612751.0
39	794643.0
40	1006209.5
41	1210972.0
42	1402833.0
43	1579873.5
44	1706446.0
45	1765712.0
46	1765731.0
47	1707444.5
48	1600110.0
49	1448817.5
50	1257888.5
51	1055193.5
52	858409.0
53	680325.0
54	529296.0
55	398828.0
56	294974.5
57	217315.0
58	157454.5
59	112527.5
60	77081.5
61	50956.0
62	33956.0
63	23263.5
64	15874.5
65	10566.5
66	7372.0
67	5492.0
68	4871.5
69	3583.5
70	1725.0
71	955.5
72	524.5
73	366.0
74	304.0
75	218.0
76	132.0
77	46.5
78	19.0
79	2.0
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	8.301375183399168E-7
125-129	4.981026605522683E-6
130-134	0.05433617008356975
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62-63	3.0
64-65	2.0
66-67	3.0
68-69	7.0
70-71	14.0
72-73	15.0
74-75	18.0
76-77	33.0
78-79	39.0
80-81	41.0
82-83	49.0
84-85	60.0
86-87	75.0
88-89	133.0
90-91	108.0
92-93	96.0
94-95	110.0
96-97	126.0
98-99	143.0
100-101	168.0
102-103	134.0
104-105	163.0
106-107	197.0
108-109	181.0
110-111	212.0
112-113	222.0
114-115	204.0
116-117	234.0
118-119	263.0
120-121	350.0
122-123	340.0
124-125	400.0
126-127	452.0
128-129	518.0
130-131	618.0
132-133	756.0
134-135	1109.0
136-137	1644.0
138-139	3509.0
140-141	7438.0
142-143	11069.0
144-145	68957.0
146-147	840645.0
148-149	1018457.0
150-151	2.2136478E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.08239373396322
#Duplication Level	Percentage of total
1	29.7455477024266
2	10.695219908394964
3	6.996414270536045
4	5.132698354807617
5	3.8699629002515294
6	2.991158033189625
7	2.5574583716000694
8	2.090995879322194
9	1.7592924311849616
>10	21.126699484735386
>50	5.316772391097832
>100	6.076822458565096
>500	0.9014780650399219
>1k	0.6512604121068248
>5k	0.08821933674121377
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	8.300204106169073E-6	1.2450306159253608E-5	4.1501020530845364E-6	0.0	5.64413879219497E-4	4.1501020530845364E-6
2	2.4900612318507217E-5	2.4900612318507217E-5	4.1501020530845364E-6	8.300204106169073E-6	0.0037890431744661817	4.1501020530845364E-6
3	3.735091847776083E-5	2.4900612318507217E-5	4.1501020530845364E-6	8.300204106169073E-6	0.005088025117081642	4.1501020530845364E-6
4	6.640163284935258E-5	2.4900612318507217E-5	4.1501020530845364E-6	8.300204106169073E-6	0.0063870070596971015	4.1501020530845364E-6
5	7.470183695552165E-5	2.4900612318507217E-5	4.1501020530845364E-6	8.300204106169073E-6	0.0077814413495335055	4.1501020530845364E-6
6	9.545234722094433E-5	3.320081642467629E-5	4.1501020530845364E-6	8.300204106169073E-6	0.009184175843476078	4.1501020530845364E-6
7	1.1205275543328249E-4	4.150102053084536E-5	4.1501020530845364E-6	8.300204106169073E-6	0.011039271461204868	4.1501020530845364E-6
8	1.8675459238880413E-4	4.150102053084536E-5	4.1501020530845364E-6	8.300204106169073E-6	0.016089945659808747	4.1501020530845364E-6
9	1.950547964949732E-4	4.150102053084536E-5	4.1501020530845364E-6	8.300204106169073E-6	0.021431127002128545	4.1501020530845364E-6
10-11	2.3863086805236085E-4	4.9801224637014434E-5	4.1501020530845364E-6	8.300204106169073E-6	0.02697981344710257	4.1501020530845364E-6
12-13	2.8013188858320623E-4	5.18762756635567E-5	4.1501020530845364E-6	1.2450306159253608E-5	0.0322193172891218	4.1501020530845364E-6
14-15	3.216329091140516E-4	6.225153079626805E-5	4.1501020530845364E-6	1.2450306159253608E-5	0.03929316623860439	4.1501020530845364E-6
16-17	3.6728403169798143E-4	6.432658182281032E-5	4.1501020530845364E-6	1.2450306159253608E-5	0.04442476742724342	4.1501020530845364E-6
18-19	4.316106135207918E-4	6.847668387589485E-5	4.1501020530845364E-6	1.2450306159253608E-5	0.05084082520131211	4.1501020530845364E-6
20-21	5.042373994497712E-4	7.677688798206392E-5	4.1501020530845364E-6	1.2450306159253608E-5	0.0558230227160401	4.1501020530845364E-6
22-23	5.623388281929547E-4	9.545234722094433E-5	4.1501020530845364E-6	1.8675459238880414E-5	0.061525262936978256	4.1501020530845364E-6
24-25	6.245903589892227E-4	9.545234722094433E-5	4.1501020530845364E-6	2.4900612318507217E-5	0.06614847662411442	4.1501020530845364E-6
26-27	6.57791175413899E-4	9.75273982474866E-5	4.1501020530845364E-6	2.4900612318507217E-5	0.06974869015516526	4.1501020530845364E-6
28-29	7.594686757144702E-4	1.0790265338019795E-4	4.1501020530845364E-6	2.4900612318507217E-5	0.07352735807449873	4.1501020530845364E-6
30-31	9.275478088643939E-4	1.2035295953945156E-4	8.300204106169073E-6	2.4900612318507217E-5	0.07770651084195486	4.1501020530845364E-6
32-33	0.0010894017889346908	1.307282146721629E-4	8.300204106169073E-6	2.9050714371591756E-5	0.08211599427335718	4.1501020530845364E-6
34-35	0.0012367304118191917	1.3280326569870517E-4	8.300204106169073E-6	2.9050714371591756E-5	0.08479488514862324	4.1501020530845364E-6
36-37	0.001301056993642002	1.4110346980487424E-4	8.300204106169073E-6	2.9050714371591756E-5	0.08762317969780037	4.1501020530845364E-6
38-39	0.0013446330651993898	1.4110346980487424E-4	8.300204106169073E-6	2.9050714371591756E-5	0.09054485154317188	4.1501020530845364E-6
40-41	0.0013819839836771506	1.431785208314165E-4	8.300204106169073E-6	2.9050714371591756E-5	0.09310338945889848	4.1501020530845364E-6
42-43	0.0014089596470222001	1.556288269906701E-4	8.300204106169073E-6	2.9050714371591756E-5	0.09565155211949239	4.1501020530845364E-6
44-45	0.0014400854124203342	1.7430428622955052E-4	8.300204106169073E-6	2.9050714371591756E-5	0.09777847942169822	4.1501020530845364E-6
46-47	0.0014836614839777219	2.1165520470731134E-4	8.300204106169073E-6	2.9050714371591756E-5	0.10001745947933732	4.1501020530845364E-6
48-49	0.0015272375555351095	2.220304598400227E-4	1.037525513271134E-5	2.9050714371591756E-5	0.10205100948534876	4.1501020530845364E-6
50-51	0.0015832639332517507	2.282556129196495E-4	1.2450306159253608E-5	2.9050714371591756E-5	0.10393723086847567	4.1501020530845364E-6
52-53	0.001647590515074561	2.3448076599927632E-4	1.2450306159253608E-5	2.9050714371591756E-5	0.10586702832315997	4.1501020530845364E-6
54-55	0.00170154184176466	2.5730632729124126E-4	1.2450306159253608E-5	2.9050714371591756E-5	0.1074482172053852	4.1501020530845364E-6
56-57	0.0017658684235874703	2.635314803708681E-4	1.2450306159253608E-5	2.9050714371591756E-5	0.10924313634334426	4.1501020530845364E-6
58-59	0.0018281199543837383	2.7805683755666394E-4	1.2450306159253608E-5	2.9050714371591756E-5	0.11086997634815339	4.1501020530845364E-6
60-61	0.0018737710769676681	2.822069396097485E-4	1.2450306159253608E-5	2.9050714371591756E-5	0.11221668446437932	8.300204106169073E-6
62-63	0.0019173471485250558	2.925821947424598E-4	1.2450306159253608E-5	3.1125765398134024E-5	0.11400122834720566	8.300204106169073E-6
64-65	0.0019235723016046828	3.0918260295479793E-4	1.2450306159253608E-5	3.320081642467629E-5	0.11547451457605068	8.300204106169073E-6
66-67	0.0019920489854805775	3.40308368352932E-4	1.4525357185795878E-5	3.320081642467629E-5	0.1166095674875693	8.300204106169073E-6
68-69	0.002019024648825627	3.527586745121856E-4	1.6600408212338146E-5	3.320081642467629E-5	0.11785252305246813	8.300204106169073E-6
70-71	0.0020356250570379647	3.548337255387279E-4	1.6600408212338146E-5	3.320081642467629E-5	0.11901870172938488	1.037525513271134E-5
72-73	0.00207712607756881	3.693590827245237E-4	1.6600408212338146E-5	3.320081642467629E-5	0.11984872214000178	1.2450306159253608E-5
74-75	0.002099951638860775	3.7350918477760826E-4	1.6600408212338146E-5	3.320081642467629E-5	0.12082607117350319	1.2450306159253608E-5
76-77	0.002126927302205825	3.901095929899464E-4	1.6600408212338146E-5	3.320081642467629E-5	0.12196942428912798	1.2450306159253608E-5
78-79	0.002212004394294058	4.025598991492E-4	2.075051026542268E-5	3.320081642467629E-5	0.12343233526284028	1.2450306159253608E-5
80-81	0.002230679853532938	4.1916030736153815E-4	2.075051026542268E-5	3.527586745121856E-5	0.12471264174621685	1.2450306159253608E-5
82-83	0.002253505414824903	4.440609196800454E-4	2.075051026542268E-5	3.735091847776083E-5	0.12617140261787607	1.6600408212338146E-5
84-85	0.0022950064353557485	4.56511225839299E-4	2.075051026542268E-5	3.9425969504303095E-5	0.1275575367036063	1.6600408212338146E-5
86-87	0.0023282072517804247	4.8141183815780624E-4	2.075051026542268E-5	4.150102053084536E-5	0.1291387255858315	1.6600408212338146E-5
88-89	0.002336507455886594	4.855619402108908E-4	2.075051026542268E-5	4.150102053084536E-5	0.13062653717186232	1.6600408212338146E-5
90-91	0.0023489577620458475	4.855619402108908E-4	2.075051026542268E-5	4.357607155738763E-5	0.1315872857971514	1.6600408212338146E-5
92-93	0.0023489577620458475	4.897120422639753E-4	2.4900612318507217E-5	4.56511225839299E-5	0.13245880722829914	1.6600408212338146E-5
94-95	0.0023821585784705238	4.938621443170599E-4	2.4900612318507217E-5	4.56511225839299E-5	0.1334050304964024	2.075051026542268E-5
96-97	0.0023863086805236086	5.125376035559402E-4	2.4900612318507217E-5	4.56511225839299E-5	0.13430767769294832	2.075051026542268E-5
98-99	0.0023883837315501508	5.208378076621093E-4	2.4900612318507217E-5	4.56511225839299E-5	0.1351999496343615	2.282556129196495E-5
100-101	0.00239668393565632	5.353631648479051E-4	2.4900612318507217E-5	4.56511225839299E-5	0.1359573432590494	2.4900612318507217E-5
102-103	0.0024049841397624887	5.415883179275319E-4	3.320081642467629E-5	4.56511225839299E-5	0.13679981397582558	2.4900612318507217E-5
104-105	0.0024112092928421157	5.436633689540742E-4	3.320081642467629E-5	4.56511225839299E-5	0.13758625831488508	2.4900612318507217E-5
106-107	0.0024361099051606228	5.623388281929547E-4	3.320081642467629E-5	4.56511225839299E-5	0.13833120163341378	2.4900612318507217E-5
108-109	0.0024402600072137076	5.685639812725814E-4	3.320081642467629E-5	4.56511225839299E-5	0.13918612265634916	2.4900612318507217E-5
110-111	0.0024485602113198763	5.830893384583774E-4	3.735091847776083E-5	4.56511225839299E-5	0.14007631954673583	2.9050714371591756E-5
112-113	0.0024589354664525877	5.893144915380042E-4	3.735091847776083E-5	4.56511225839299E-5	0.1408316381203972	2.9050714371591756E-5
114-115	0.0024651606195322147	5.893144915380042E-4	3.735091847776083E-5	4.56511225839299E-5	0.14155168082660735	2.9050714371591756E-5
116-117	0.0024713857726118412	5.893144915380042E-4	3.735091847776083E-5	4.56511225839299E-5	0.14240452679851623	2.9050714371591756E-5
118-119	0.002473460823638384	5.934645935910888E-4	4.150102053084536E-5	4.56511225839299E-5	0.14314532001499183	2.9050714371591756E-5
120-121	0.0024776109256914682	5.976146956441732E-4	4.56511225839299E-5	4.56511225839299E-5	0.14385083736401622	2.9050714371591756E-5
122-123	0.0024817610277445526	5.976146956441732E-4	4.56511225839299E-5	4.56511225839299E-5	0.1445916305804918	2.9050714371591756E-5
124-125	0.0024879861808241796	6.079899507768846E-4	4.56511225839299E-5	4.56511225839299E-5	0.14540090048084328	3.735091847776083E-5
126-127	0.0025025115380099753	6.328905630953918E-4	4.56511225839299E-5	4.56511225839299E-5	0.1463305233407342	4.150102053084536E-5
128-129	0.002521186997248856	6.432658182281032E-4	4.56511225839299E-5	4.56511225839299E-5	0.1472435457924128	4.9801224637014434E-5
130-131	0.0025315622523815673	6.515660223342721E-4	4.56511225839299E-5	4.56511225839299E-5	0.14815034309101177	4.9801224637014434E-5
132-133	0.0025730632729124127	6.640163284935258E-4	4.56511225839299E-5	4.56511225839299E-5	0.14899903896086758	5.810142874318351E-5
134-135	0.0025896636811247506	6.640163284935258E-4	4.56511225839299E-5	4.7726173610472166E-5	0.14973983217734316	6.225153079626805E-5
136-137	0.0026083391403636308	6.723165325996949E-4	4.56511225839299E-5	4.9801224637014434E-5	0.15044742457739407	6.640163284935258E-5
138-139	0.0026145642934432578	6.847668387589485E-4	4.56511225839299E-5	4.9801224637014434E-5	0.15114256667128573	7.055173490243712E-5
>>END_MODULE
