##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.control.4d.R.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24236066
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	66-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.903174879949574	37.0	35.0	38.0	33.0	39.0
2	35.27268249723367	35.0	34.0	37.0	33.0	39.0
3	35.42537113902892	35.0	34.0	37.0	33.0	39.0
4	35.46533529822868	36.0	35.0	37.0	33.0	39.0
5	35.38158342199596	36.0	35.0	37.0	33.0	39.0
6	35.34840633789329	35.0	34.0	37.0	33.0	39.0
7	35.36852990085107	35.0	34.0	37.0	33.0	39.0
8	35.48963519904592	36.0	34.0	38.0	33.0	39.0
9	35.444356480956934	36.0	34.0	38.0	33.0	39.0
10-14	35.432083606308055	35.8	34.4	37.8	33.0	39.0
15-19	35.409312055842726	36.0	34.0	38.0	33.0	39.0
20-24	35.390466571596235	36.0	34.0	38.0	33.0	39.0
25-29	35.368400638948586	36.0	34.0	38.0	33.0	39.0
30-34	35.3514710514487	36.0	34.0	37.8	33.0	39.0
35-39	35.32130564424111	36.0	34.0	37.6	32.6	39.0
40-44	35.30138883926129	35.8	34.0	37.4	32.0	39.0
45-49	35.27042973888583	35.8	34.0	37.2	32.0	39.0
50-54	35.24263136599809	35.6	34.0	37.0	32.0	39.0
55-59	35.22077450193443	35.8	34.0	37.2	32.0	39.0
60-64	35.17684058130557	35.0	34.0	37.0	32.0	39.0
65-69	35.147734095807074	35.0	34.0	37.0	32.0	39.0
70-74	35.110247375955126	35.0	34.0	37.0	32.0	39.0
75-79	35.06590608882097	35.0	34.0	37.0	32.0	39.0
80-84	35.02374548248506	35.0	34.0	37.0	32.0	39.0
85-89	34.97024889142621	35.0	34.0	37.0	32.0	39.0
90-94	34.91138274951557	35.0	34.0	37.0	31.0	39.0
95-99	34.84403667702835	35.0	34.0	37.0	31.0	39.0
100-104	34.79530132291909	35.0	34.0	37.0	31.0	39.0
105-109	34.73179813637834	35.0	34.0	37.0	31.0	39.0
110-114	34.658192755391234	35.0	34.0	37.0	30.6	39.0
115-119	34.58780860497761	35.0	34.0	37.0	30.0	39.0
120-124	34.50807332414407	35.0	34.0	37.0	30.0	39.0
125-129	34.426168699701805	35.0	34.0	37.0	30.0	39.0
130-134	34.33901899787054	35.0	34.0	37.0	30.0	39.0
135-139	34.261625643935716	35.0	34.0	37.0	29.4	39.0
140-144	34.13466938723005	35.0	34.0	37.0	29.0	39.0
145-149	34.123462992309484	35.0	34.0	37.0	29.0	39.0
150	34.94181790120229	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	6.0
21	153.0
22	1492.0
23	8583.0
24	30251.0
25	76600.0
26	153810.0
27	265060.0
28	393313.0
29	521650.0
30	671044.0
31	877182.0
32	1177616.0
33	1647837.0
34	2475370.0
35	4193543.0
36	1.0221839E7
37	1520707.0
38	10.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.094364407160796	19.454898332097297	16.189483887360268	31.261253373381635
2	23.53884908549102	26.545025913033903	33.10167169869895	16.81445330277612
3	19.624389535826484	30.219174184457167	29.70357895542948	20.45285732428687
4	22.88368087461059	34.76152854180212	22.475863038167994	19.878927545419295
5	24.536271686997384	35.47635990098393	22.79511039456651	17.192258017452172
6	18.75889428589607	38.83999160589842	23.242237415923857	19.15887669228166
7	20.04070710155683	20.677056251621035	38.283028276948905	20.99920836987323
8	21.493401610640937	23.360742622173085	28.430670225109967	26.715185542076014
9	23.326232070831956	23.380023804193304	28.754542919630605	24.539201205344135
10-14	23.03713647256118	29.096502707988996	25.76575010152225	22.100610717927573
15-19	22.81174015617881	28.30300099034224	27.234063482084924	21.65119537139402
20-24	22.91184138547898	28.386286784332075	27.12246203653679	21.579409793652154
25-29	23.031982995920213	28.361356170593034	27.03572601262928	21.570934820857477
30-34	23.074717654259565	28.13110675635229	27.16043106995995	21.633744519428195
35-39	23.14111786954203	28.106171191314626	27.143240161171374	21.60947077797197
40-44	23.301289078846377	27.872202526598173	27.26463279972913	21.561875594826322
45-49	23.325051186112468	27.701072443027673	27.316387073710725	21.657489297149134
50-54	23.40994615215192	27.646562771367268	27.290921719721347	21.652569356759468
55-59	23.55837783244195	27.538783728349316	27.1791189213629	21.72371951784584
60-64	23.490466645865713	27.488910122624688	27.23845941003792	21.782163821471688
65-69	23.57094161116164	27.420593169263675	27.194158708182297	21.814306511392388
70-74	23.64823827041361	27.383263303209386	27.091143050460577	21.87735537591643
75-79	23.58222241491997	27.42862557235212	27.162390200210528	21.826761812517383
80-84	23.686285785245353	27.461392166229498	27.042130837387486	21.810191211137667
85-89	23.731919671294314	27.375745219060516	27.0921073173213	21.800227792323867
90-94	23.667007159425545	27.32302216289319	27.127985665964555	21.881985011716715
95-99	23.745592620490243	27.454011300248155	27.093794000657525	21.706602078604078
100-104	23.902771145771588	27.433666507003363	26.88703487333943	21.77652747388562
105-109	23.690461586022877	27.559669890899634	26.987404748090217	21.76246377498727
110-114	23.72600425736342	27.582664989338433	26.90022991875225	21.791100834545897
115-119	23.81282302569292	27.580254554366444	26.905426705780545	21.70149571416009
120-124	23.787966273953234	27.492505497179238	27.082655522591466	21.63687270627607
125-129	23.765140767153937	27.56429355357178	27.039518172964915	21.63104750630937
130-134	23.811014721508474	27.474298550899938	27.127229287167026	21.587457440424558
135-139	23.74350559792235	27.48266036478595	27.209157502148134	21.56467653514357
140-144	23.78221249756652	27.577715498646626	27.170571483391498	21.46950052039536
145-149	23.503526636221586	27.55437406114044	27.220701149801986	21.72139815283599
150	23.61121278155551	26.391261867044385	27.65161527390979	22.34591007749031
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.5
4	0.5
5	0.0
6	0.0
7	0.0
8	1.5
9	5.0
10	7.0
11	5.0
12	7.5
13	16.0
14	32.5
15	46.0
16	70.5
17	144.5
18	230.0
19	413.5
20	655.5
21	977.5
22	1542.0
23	2585.5
24	4303.5
25	7079.5
26	11279.0
27	18206.5
28	30534.0
29	46602.5
30	61577.5
31	80448.5
32	105968.0
33	138591.5
34	187211.0
35	259220.0
36	361106.5
37	488182.0
38	638592.5
39	825006.5
40	1038685.0
41	1237556.0
42	1416435.5
43	1581428.0
44	1697931.5
45	1748322.0
46	1744150.5
47	1685241.0
48	1576532.5
49	1430273.5
50	1243274.0
51	1042598.0
52	854356.0
53	681459.0
54	531611.5
55	403237.5
56	300923.0
57	223401.0
58	162219.0
59	116176.0
60	79849.5
61	52844.5
62	35119.0
63	24418.0
64	17030.5
65	11431.0
66	8079.0
67	6078.0
68	5489.0
69	4062.5
70	1993.5
71	1185.0
72	670.5
73	503.0
74	420.0
75	287.0
76	196.0
77	70.0
78	19.0
79	4.0
80	3.5
81	1.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	8.252165106117602E-7
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	8.252768704867258E-7
110-114	0.0
115-119	0.0
120-124	8.253403181444277E-7
125-129	8.253741745092414E-7
130-134	0.0
135-139	8.25521976100825E-7
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
66-67	5.0
68-69	4.0
70-71	10.0
72-73	12.0
74-75	24.0
76-77	27.0
78-79	32.0
80-81	36.0
82-83	57.0
84-85	52.0
86-87	83.0
88-89	127.0
90-91	121.0
92-93	147.0
94-95	108.0
96-97	136.0
98-99	168.0
100-101	169.0
102-103	171.0
104-105	195.0
106-107	182.0
108-109	206.0
110-111	209.0
112-113	232.0
114-115	248.0
116-117	266.0
118-119	271.0
120-121	341.0
122-123	347.0
124-125	380.0
126-127	499.0
128-129	535.0
130-131	654.0
132-133	792.0
134-135	1141.0
136-137	1697.0
138-139	3632.0
140-141	7498.0
142-143	11810.0
144-145	73398.0
146-147	842813.0
148-149	1102263.0
150-151	2.2184968E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.85436425006752
#Duplication Level	Percentage of total
1	29.88430448295642
2	10.304179507473501
3	6.812063050075409
4	4.879589030996033
5	3.800331697447594
6	3.0047822911176847
7	2.471093748900183
8	2.0351148439292883
9	1.760599709957628
>10	20.80301168967274
>50	5.2270523154531
>100	6.618672083150807
>500	1.2065761061145657
>1k	1.0378067656805827
>5k	0.10481041236112768
>10k+	0.05001226471330979
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.237824653555573E-5	4.126082178518576E-6	0.0	0.0	7.839556139185295E-4	0.0
2	3.7134739606667184E-5	3.300865742814861E-5	0.0	0.0	0.005561958776643041	0.0
3	7.839556139185295E-5	3.300865742814861E-5	0.0	0.0	0.007406317510440845	0.0
4	1.4441287624815017E-4	3.300865742814861E-5	0.0	0.0	0.008961850491742349	0.0
5	1.4441287624815017E-4	3.300865742814861E-5	0.0	4.126082178518576E-6	0.010542139966114963	0.0
6	1.567911227837059E-4	3.300865742814861E-5	0.0	4.126082178518576E-6	0.012192572837522394	0.0
7	1.732954514977802E-4	4.1260821785185766E-5	0.0	4.126082178518576E-6	0.014449539789172055	0.0
8	2.269345198185217E-4	4.538690396370434E-5	0.0	4.126082178518576E-6	0.020506628427237324	0.0
9	2.3518668417555887E-4	4.951298614222292E-5	0.0	4.126082178518576E-6	0.027075351255438897	0.0
10-11	2.8676271140704105E-4	5.363906832074149E-5	4.126082178518576E-6	4.126082178518576E-6	0.033722469645032324	0.0
12-13	3.238974510137082E-4	6.395427376703793E-5	4.126082178518576E-6	4.126082178518576E-6	0.0400580688301476	2.063041089259288E-6
14-15	3.775365193344497E-4	7.839556139185295E-5	4.126082178518576E-6	4.126082178518576E-6	0.04910656704763884	4.126082178518576E-6
16-17	4.2498646438741337E-4	8.252164357037153E-5	4.126082178518576E-6	4.126082178518576E-6	0.0549057755495467	4.126082178518576E-6
18-19	4.930668203329699E-4	8.871076683814939E-5	4.126082178518576E-6	4.126082178518576E-6	0.06320538985163682	4.126082178518576E-6
20-21	5.5083197083223E-4	9.696293119518655E-5	4.126082178518576E-6	4.126082178518576E-6	0.06988964298083691	4.126082178518576E-6
22-23	6.189123267777864E-4	1.237824653555573E-4	4.126082178518576E-6	4.126082178518576E-6	0.07656151786350145	4.126082178518576E-6
24-25	6.973078881696394E-4	1.2997158862333518E-4	4.126082178518576E-6	4.126082178518576E-6	0.08217711570846523	4.126082178518576E-6
26-27	7.550730386688994E-4	1.3616071189111303E-4	4.126082178518576E-6	6.189123267777865E-6	0.08648268246174937	4.126082178518576E-6
28-29	8.726663807566789E-4	1.567911227837059E-4	4.126082178518576E-6	8.252164357037152E-6	0.0910915162551546	4.126082178518576E-6
30-31	0.0010707183253255705	1.6916936931926163E-4	1.237824653555573E-5	8.252164357037152E-6	0.09613152563621505	4.126082178518576E-6
32-33	0.0012440137768233508	1.7948457476555808E-4	1.237824653555573E-5	8.252164357037152E-6	0.10112408507222254	4.126082178518576E-6
34-35	0.0013884266530715009	1.8773673912259522E-4	1.237824653555573E-5	8.252164357037152E-6	0.1044497073081085	4.126082178518576E-6
36-37	0.0014730113377311319	1.939258623903731E-4	1.237824653555573E-5	8.252164357037152E-6	0.10803320968015188	4.126082178518576E-6
38-39	0.0015472808169444661	2.0011498565815095E-4	1.237824653555573E-5	8.252164357037152E-6	0.11140834490218007	4.126082178518576E-6
40-41	0.0015741003511048368	2.1661931437222528E-4	1.237824653555573E-5	1.0315205446296442E-5	0.11424296335882234	4.126082178518576E-6
42-43	0.0016071090085329855	2.2899756090778099E-4	1.237824653555573E-5	1.237824653555573E-5	0.11718485995210609	4.126082178518576E-6
44-45	0.0016483698303181712	2.3931276635407743E-4	1.237824653555573E-5	1.237824653555573E-5	0.1198193634230902	4.126082178518576E-6
46-47	0.0017205762684422464	2.8469967031778177E-4	1.237824653555573E-5	1.237824653555573E-5	0.1224744973049669	4.126082178518576E-6
48-49	0.0017845305422092843	2.8882575249630034E-4	1.237824653555573E-5	1.237824653555573E-5	0.12487794017395397	4.126082178518576E-6
50-51	0.0018422956927085445	2.8882575249630034E-4	1.237824653555573E-5	1.237824653555573E-5	0.12713284408451436	4.126082178518576E-6
52-53	0.0019310064595466938	2.929518346748189E-4	1.237824653555573E-5	1.237824653555573E-5	0.12942488273468145	4.126082178518576E-6
54-55	0.001980519445688917	3.0739312229963395E-4	1.4441287624815017E-5	1.237824653555573E-5	0.13166328231652777	4.126082178518576E-6
56-57	0.0020795454179733624	3.1977136883518966E-4	1.8567369803333592E-5	1.237824653555573E-5	0.13384185370678559	4.126082178518576E-6
58-59	0.0021930126778826236	3.4246482081704184E-4	2.0630410892592883E-5	1.237824653555573E-5	0.13605755983665005	4.126082178518576E-6
60-61	0.0023085429788811435	3.7959956042370906E-4	2.0630410892592883E-5	1.237824653555573E-5	0.13756564287289857	4.126082178518576E-6
62-63	0.002353929882844848	4.0848213567333904E-4	2.0630410892592883E-5	1.237824653555573E-5	0.13968232303047862	4.126082178518576E-6
64-65	0.0023807494170052187	4.2498646438741337E-4	2.0630410892592883E-5	1.237824653555573E-5	0.14152461872318717	4.126082178518576E-6
66-67	0.0024653341016648493	4.414907931014877E-4	2.0630410892592883E-5	1.237824653555573E-5	0.1428717845544735	4.126082178518576E-6
68-69	0.0024983427590929983	4.4974295745852484E-4	2.0630410892592883E-5	1.237824653555573E-5	0.14420863518031352	4.126082178518576E-6
70-71	0.002531351416521147	4.6831032726185845E-4	2.0630410892592883E-5	1.237824653555573E-5	0.14551454018981463	4.126082178518576E-6
72-73	0.002574675279395592	4.848146559759327E-4	2.0630410892592883E-5	1.237824653555573E-5	0.14648416950176651	4.126082178518576E-6
74-75	0.002599431772466703	5.033820257792663E-4	2.269345198185217E-5	1.237824653555573E-5	0.14764359859393023	4.126082178518576E-6
76-77	0.0026365665120733706	5.322646010288963E-4	2.475649307111146E-5	1.237824653555573E-5	0.1489515666445206	4.126082178518576E-6
78-79	0.002684016457126334	5.528950119214893E-4	2.475649307111146E-5	1.237824653555573E-5	0.1506040625570173	4.126082178518576E-6
80-81	0.0026963947036618896	5.65273258457045E-4	2.8882575249630034E-5	1.237824653555573E-5	0.15193678710067882	4.126082178518576E-6
82-83	0.002714962073465223	5.776515049926007E-4	2.8882575249630034E-5	1.237824653555573E-5	0.15338916802751734	8.252164357037152E-6
84-85	0.0027417816076255944	5.859036693496378E-4	2.8882575249630034E-5	1.237824653555573E-5	0.15479616205039218	8.252164357037152E-6
86-87	0.002768601141785965	5.982819158851936E-4	2.8882575249630034E-5	1.6504328714074305E-5	0.15652499048319146	8.252164357037152E-6
88-89	0.0027871685115892983	5.982819158851936E-4	3.7134739606667184E-5	1.6504328714074305E-5	0.15829920581995444	1.237824653555573E-5
90-91	0.002799546758124854	6.189123267777865E-4	4.1260821785185766E-5	1.6504328714074305E-5	0.15915743091308632	1.237824653555573E-5
92-93	0.002805735881392632	6.189123267777865E-4	4.332386287444505E-5	1.6504328714074305E-5	0.1601765732111804	1.4441287624815017E-5
94-95	0.0028181141279281876	6.209753678670458E-4	4.538690396370434E-5	1.6504328714074305E-5	0.1612142828790778	1.6504328714074305E-5
96-97	0.002820177169017447	6.271644911348236E-4	4.538690396370434E-5	1.6504328714074305E-5	0.16208488621874523	1.6504328714074305E-5
98-99	0.0028469967031778177	6.416057787596385E-4	4.538690396370434E-5	1.8567369803333592E-5	0.16288534616137784	1.6504328714074305E-5
100-101	0.0028531858264455954	6.581101074737129E-4	4.951298614222292E-5	2.0630410892592883E-5	0.16358471709063674	1.6504328714074305E-5
102-103	0.0028593749497133735	6.663622718307502E-4	4.951298614222292E-5	2.269345198185217E-5	0.16440580744416194	1.6504328714074305E-5
104-105	0.0028676271140704104	6.684253129200094E-4	4.951298614222292E-5	2.475649307111146E-5	0.16519595218134825	1.6504328714074305E-5
106-107	0.0028882575249630036	6.746144361877873E-4	4.951298614222292E-5	2.6819534160370746E-5	0.1659468991378386	1.6504328714074305E-5
108-109	0.0028882575249630036	6.869926827233429E-4	4.951298614222292E-5	3.300865742814861E-5	0.16668546784779345	1.8567369803333592E-5
110-111	0.002892383607141522	7.055600525266766E-4	4.951298614222292E-5	3.300865742814861E-5	0.16749005387260457	2.0630410892592883E-5
112-113	0.0029006357714985594	7.468208743118623E-4	4.951298614222292E-5	3.300865742814861E-5	0.1682121182538453	2.0630410892592883E-5
114-115	0.002906824894766337	7.633252030259366E-4	4.951298614222292E-5	3.300865742814861E-5	0.16896719129251422	2.0630410892592883E-5
116-117	0.002915077059123374	7.653882441151959E-4	5.363906832074149E-5	3.50716985174079E-5	0.1698398576732709	2.0630410892592883E-5
118-119	0.0029253922645696706	7.674512852044553E-4	5.363906832074149E-5	3.7134739606667184E-5	0.17060730895847537	2.0630410892592883E-5
120-121	0.002929518346748189	7.715773673829738E-4	5.363906832074149E-5	3.7134739606667184E-5	0.1715067948733924	2.0630410892592883E-5
122-123	0.0029377705111052264	7.757034495614924E-4	5.5702109410000783E-5	3.7134739606667184E-5	0.17227630919968612	2.0630410892592883E-5
124-125	0.0029480857165515226	7.839556139185295E-4	5.776515049926007E-5	3.7134739606667184E-5	0.17305820177251538	2.475649307111146E-5
126-127	0.0029625270041763376	7.983969015433446E-4	5.776515049926007E-5	3.9197780695926475E-5	0.17390817470129022	2.6819534160370746E-5
128-129	0.00298522045615819	8.045860248111224E-4	5.982819158851936E-5	4.1260821785185766E-5	0.17478496716422542	3.300865742814861E-5
130-131	0.0029914095794259677	8.190273124359373E-4	6.189123267777865E-5	4.1260821785185766E-5	0.17570095740785652	3.300865742814861E-5
132-133	0.003020292154675598	8.210903535251967E-4	6.189123267777865E-5	4.1260821785185766E-5	0.17647872389850727	3.300865742814861E-5
134-135	0.00304298560665745	8.23153394614456E-4	6.189123267777865E-5	4.1260821785185766E-5	0.17728743600549693	3.7134739606667184E-5
136-137	0.0030533008121037463	8.314055589714931E-4	6.189123267777865E-5	4.1260821785185766E-5	0.1780239416743625	3.9197780695926475E-5
138-139	0.0030574268942822652	8.623511753103824E-4	6.189123267777865E-5	4.1260821785185766E-5	0.17877695167194213	4.1260821785185766E-5
>>END_MODULE
