##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.control.4d.R.3.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24130301
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	75-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.436372302193824	36.0	34.0	38.0	31.0	39.0
2	34.681857677614545	35.0	34.0	37.0	31.0	39.0
3	34.84971990196061	35.0	34.0	38.0	31.0	39.0
4	34.848669521362375	35.0	34.0	38.0	31.0	39.0
5	34.71714646244985	35.0	34.0	37.0	31.0	39.0
6	34.701097222119195	35.0	34.0	37.0	31.0	39.0
7	34.736627860547614	35.0	34.0	37.0	32.0	39.0
8	34.845254851980506	35.0	34.0	37.0	32.0	39.0
9	34.81946114140889	35.0	34.0	37.0	32.0	39.0
10-14	34.80331554919269	35.0	34.0	37.0	31.2	39.0
15-19	34.82250785019217	35.0	34.0	37.0	31.0	39.0
20-24	34.81268539501435	35.0	34.0	37.0	31.0	39.0
25-29	34.80980811635959	35.0	34.0	37.0	31.0	39.0
30-34	34.79581752419914	35.0	34.0	37.0	31.0	39.0
35-39	34.7869807591708	35.0	34.0	37.0	31.0	39.0
40-44	34.74580693378006	35.0	34.0	37.0	31.0	39.0
45-49	34.64954137952941	35.0	34.0	37.0	31.0	39.0
50-54	34.62560089076386	35.0	34.0	37.0	31.0	39.0
55-59	34.611627770411985	35.0	34.0	37.0	31.0	39.0
60-64	34.59071364256915	35.0	34.0	37.0	31.0	39.0
65-69	34.55209332863274	35.0	34.0	37.0	30.8	39.0
70-74	34.55824362074886	35.0	34.0	37.0	30.6	39.0
75-79	34.539503276889015	35.0	34.0	37.0	30.0	39.0
80-84	34.52896204429628	35.0	34.0	37.0	30.0	39.0
85-89	34.50636447825413	35.0	34.0	37.0	30.0	39.0
90-94	34.486602101039566	35.0	34.0	37.0	30.0	39.0
95-99	34.46896563774943	35.0	34.0	37.0	30.0	39.0
100-104	34.43226257668574	35.0	34.0	37.0	30.0	39.0
105-109	34.406688310931216	35.0	34.0	37.0	30.0	39.0
110-114	34.372689660359654	35.0	34.0	37.0	30.0	39.0
115-119	34.336070167925435	35.0	34.0	37.0	30.0	39.0
120-124	34.29648101755227	35.0	34.0	37.0	30.0	39.0
125-129	34.2843305736903	35.0	34.0	37.0	30.0	39.0
130-134	34.2324581090241	35.0	34.0	37.0	30.0	39.0
135-139	34.19747336728496	35.0	34.0	37.0	29.8	39.0
140-144	34.150991465678274	35.0	34.0	37.0	29.0	39.0
145-149	34.1889198459589	35.0	34.0	37.0	29.2	39.0
150	35.043655983534016	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	12.0
22	207.0
23	2395.0
24	17621.0
25	73944.0
26	196502.0
27	366787.0
28	549869.0
29	723767.0
30	910874.0
31	1148097.0
32	1480868.0
33	1961480.0
34	2739425.0
35	4306821.0
36	8635984.0
37	1015638.0
38	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.25039327109927	10.822471713054885	13.07940999161179	40.84772502423405
2	21.82237729982215	12.14359141403875	38.36548126791479	27.668550018224312
3	20.107902603866123	16.28107334691056	28.481553707187405	35.12947034203591
4	25.624067690898674	24.280626723068842	22.871749285907434	27.22355630012505
5	25.80692391468313	29.916797484322604	25.07447074795651	19.20180785303776
6	19.206534759933596	35.734940851499374	26.711258492544758	18.347265896022268
7	15.954345338233674	25.09921039059423	40.21802892091826	18.72841535025384
8	17.962048159767487	24.430447637833748	32.41287103921689	25.194633163181873
9	19.138154798331538	22.973958893875754	33.56676901251801	24.321117295274693
10-14	20.196778213585713	28.990348820467798	27.377903205207225	23.434969760739264
15-19	20.388505311539916	27.788381395624935	27.94905037347794	23.874062919357208
20-24	20.55439780941888	27.9334144432512	27.677156501445616	23.835031245884313
25-29	20.514365250820354	28.148534361220523	27.585644402633896	23.751455985325222
30-34	20.53244646820309	27.82715860490062	27.587899464563918	24.052495462332367
35-39	20.62072651316213	27.84229375803571	27.629882508299886	23.907097220502273
40-44	20.657716445555955	27.95128421960128	27.5207786626391	23.870220672203665
45-49	20.7633713009943	27.718674566171593	27.474101102322386	24.043853030511723
50-54	20.901004644065964	27.68670118976751	27.562647083369214	23.849647082797315
55-59	20.90265613075271	27.74678474941266	27.46045636116377	23.89010275867086
60-64	20.834308388751044	27.64932040246707	27.47531144763326	24.041059761148624
65-69	20.957255608817732	27.61089875184123	27.561245942509128	23.870599696831913
70-74	21.050094471839785	27.581327202806616	27.52200832122012	23.846570004133483
75-79	21.05130260808061	27.458731247161033	27.511899329880585	23.978066814877767
80-84	21.125796115217906	27.480133686694213	27.497401624576945	23.896668573510933
85-89	21.09837230059236	27.493681772976046	27.53344511383799	23.874500812593606
90-94	21.081159152948217	27.36269470351278	27.513587605175964	24.04255853836304
95-99	21.19477714739576	27.281155112163923	27.63676923296501	23.887298507475304
100-104	21.23207183072029	27.397922867863244	27.477934787223163	23.892070514193303
105-109	21.190568695111264	27.20725949142698	27.57546571051508	24.026706102946676
110-114	21.27848078456854	27.202816175807392	27.641363743382563	23.8773392962415
115-119	21.331346769746435	27.24095514821137	27.577120623240205	23.85057745880199
120-124	21.283244274634846	27.15031590449436	27.582959270277847	23.983480550592944
125-129	21.30970364025147	27.09760733998713	27.665952957527306	23.92673606223409
130-134	21.38428865048545	27.23970077000209	27.52334059846215	23.85266998105031
135-139	21.39559120811259	27.08007671608727	27.615199400694422	23.909132675105713
140-144	21.47090025834459	27.050843701714417	27.740175829947145	23.73808020999385
145-149	21.084789990623857	27.185852257929206	27.856164154607143	23.87319359683979
150	21.076835501456408	25.689785520273468	28.510722113073495	24.722656865196633
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.5
9	2.0
10	4.0
11	5.5
12	7.5
13	21.0
14	35.5
15	71.5
16	148.5
17	305.0
18	612.5
19	1134.5
20	2112.0
21	3502.5
22	5374.5
23	8446.5
24	13278.0
25	20608.0
26	30354.5
27	43041.5
28	58891.5
29	82610.5
30	113397.0
31	145328.0
32	176168.0
33	210506.5
34	250177.5
35	298647.5
36	373896.5
37	478110.5
38	601490.5
39	751087.5
40	922748.5
41	1091793.0
42	1265101.0
43	1443493.0
44	1587929.0
45	1676649.0
46	1700415.0
47	1656884.5
48	1566801.0
49	1437910.0
50	1268762.0
51	1085459.5
52	901000.0
53	717270.0
54	552182.5
55	414850.0
56	310075.0
57	232018.5
58	172724.5
59	128196.0
60	91127.5
61	67286.5
62	49946.5
63	32482.5
64	21343.5
65	17613.0
66	19168.5
67	14602.5
68	6662.5
69	3347.5
70	2292.0
71	1434.5
72	752.0
73	323.5
74	117.0
75	78.0
76	41.0
77	30.5
78	13.0
79	1.0
80	1.0
81	1.5
82	1.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0018482985355217907
3	0.14526549005750075
4	1.077483451201044E-4
5	8.70275095200843E-5
6	7.873917528007628E-5
7	6.630667392006424E-5
8	1.4504584920014052E-4
9	1.1603667936011241E-4
10-14	2.0057768860819435E-4
15-19	2.784880304642698E-4
20-24	3.439658709603332E-4
25-29	3.249027022083148E-4
30-34	2.6108252856025296E-4
35-39	1.4421701577613973E-4
40-44	2.760015301922674E-4
45-49	1.9146052094418548E-4
50-54	9.780234403209475E-5
55-59	1.4753234947214293E-4
60-64	2.0140652203219512E-4
65-69	0.05819322353252037
70-74	1.7239735219216703E-4
75-79	1.3675752402806903E-4
80-84	9.531587615049577E-5
85-89	7.459506009465069E-5
90-94	1.4421724886368826E-4
95-99	1.0526217288857695E-4
100-104	7.210889278278753E-5
105-109	1.0774928283527818E-4
110-114	6.464984994769827E-5
115-119	4.9730925440387856E-5
120-124	9.946252853258763E-5
125-129	1.1935614224238412E-4
130-134	3.647054600162173E-5
135-139	4.641922088636823E-5
140-144	4.9752747914750396E-5
145-149	4.0569215506233705E-5
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
74-75	1.0
76-77	2.0
78-79	3.0
80-81	2.0
82-83	3.0
84-85	2.0
86-87	6.0
88-89	9.0
90-91	10.0
92-93	14.0
94-95	15.0
96-97	17.0
98-99	19.0
100-101	25.0
102-103	32.0
104-105	32.0
106-107	32.0
108-109	39.0
110-111	53.0
112-113	50.0
114-115	44.0
116-117	69.0
118-119	72.0
120-121	55.0
122-123	82.0
124-125	75.0
126-127	133.0
128-129	138.0
130-131	185.0
132-133	272.0
134-135	392.0
136-137	753.0
138-139	2284.0
140-141	5368.0
142-143	8696.0
144-145	74080.0
146-147	775754.0
148-149	1374426.0
150-151	2.1887057E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.47223617001389
#Duplication Level	Percentage of total
1	39.39921470413141
2	10.060266451322702
3	6.177504830791931
4	4.356559996558847
5	3.255649718506364
6	2.6253158261527982
7	2.207099402301623
8	1.8913118997233132
9	1.5526574082302342
>10	17.862640278350035
>50	4.688161847191258
>100	4.987370733993125
>500	0.4669922443400735
>1k	0.46925465840640895
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	8.28833424000803E-6	2.0720835600020073E-5	0.0	4.144167120004015E-6	1.9892002176019271E-4	8.28833424000803E-6
2	8.28833424000803E-6	7.045084104006826E-5	0.0	8.28833424000803E-6	0.0018607310368818028	3.315333696003212E-5
3	8.28833424000803E-6	7.873917528007628E-5	0.0	8.28833424000803E-6	0.0029133494853628224	6.216250680006022E-5
4	2.9009169840028105E-5	9.531584376009234E-5	0.0	1.2432501360012045E-5	0.004430114651284292	7.873917528007628E-5
5	2.9009169840028105E-5	9.946001088009636E-5	0.0	1.2432501360012045E-5	0.00551174226960534	8.70275095200843E-5
6	5.387417256005219E-5	1.0774834512010438E-4	0.0	1.2432501360012045E-5	0.006556072383846352	8.70275095200843E-5
7	5.387417256005219E-5	1.6576668480016059E-4	0.0	1.2432501360012045E-5	0.00772058334456748	9.117167664008833E-5
8	7.873917528007628E-5	1.740550190401686E-4	0.0	1.2432501360012045E-5	0.009821676074409515	9.946001088009636E-5
9	9.117167664008833E-5	1.740550190401686E-4	0.0	1.2432501360012045E-5	0.012710160557052314	1.118925122401084E-4
10-11	1.2846918072012445E-4	1.8027126972017466E-4	0.0	1.4504584920014053E-5	0.01710712187137657	1.4919001632014454E-4
12-13	1.5747835056015256E-4	1.8441543684017867E-4	0.0	1.657666848001606E-5	0.02094462062450029	1.6162251768015657E-4
14-15	1.7198293548016663E-4	1.968479382001907E-4	0.0	1.657666848001606E-5	0.025124013165024342	1.8027126972017466E-4
16-17	2.0720835600020074E-4	2.2171294092021478E-4	0.0	1.657666848001606E-5	0.029108629850908202	1.8648752040018068E-4
18-19	2.3414544228022682E-4	2.3621752584022885E-4	0.0	1.657666848001606E-5	0.03276378525075174	1.8648752040018068E-4
20-21	3.3567753672032524E-4	2.83875447720275E-4	0.0	1.657666848001606E-5	0.035973442685194854	1.947758546401887E-4
22-23	4.206329626804075E-4	3.004521162002911E-4	0.0	1.657666848001606E-5	0.039649318920638416	2.1342460668020676E-4
24-25	5.118046393204959E-4	3.149567011203051E-4	0.0	1.657666848001606E-5	0.04380591854200244	2.1549669024020876E-4
26-27	5.988321488405801E-4	3.149567011203051E-4	0.0	1.657666848001606E-5	0.04800810400168651	2.1964085736021278E-4
28-29	6.423459036006223E-4	3.1910086824030913E-4	0.0	1.657666848001606E-5	0.05203416235877041	2.403616929602329E-4
30-31	7.107246610806885E-4	3.2324503536031315E-4	0.0	3.936958764003815E-5	0.055819859022894075	2.5693836144024895E-4
32-33	7.666709172007428E-4	3.2324503536031315E-4	0.0	4.973000544004818E-5	0.059369338161177516	3.108125340003011E-4
34-35	8.28833424000803E-4	3.2324503536031315E-4	0.0	6.216250680006022E-5	0.06258521184630063	3.2946128604031917E-4
36-37	8.37121758240811E-4	3.2324503536031315E-4	0.0	7.459500816007227E-5	0.06549027299742344	3.439658709603332E-4
38-39	8.640588445208371E-4	3.2738920248031716E-4	0.0	8.288334240008029E-5	0.06936506925462721	3.481100380803373E-4
40-41	9.096446828408813E-4	3.2738920248031716E-4	0.0	9.117167664008833E-5	0.07257265460551031	3.543262887603433E-4
42-43	9.137888499608853E-4	3.2946128604031917E-4	0.0	9.946001088009636E-5	0.07568285202907332	3.6468670656035333E-4
44-45	9.552305211609255E-4	3.418937874003312E-4	0.0	1.0153209444009836E-4	0.07955143203559707	3.6883087368035734E-4
46-47	0.0010091046937209776	3.439658709603332E-4	0.0	1.0360417800010037E-4	0.08317136201492058	3.7297504080036135E-4
48-49	0.0010443301142410118	3.563983723203453E-4	0.0	1.0360417800010037E-4	0.08689282408868418	3.854075421603734E-4
50-51	0.001071267200521038	3.6468670656035333E-4	0.0	1.0360417800010037E-4	0.09068680908704785	3.916237928403794E-4
52-53	0.0011209972059610862	3.6883087368035734E-4	0.0	1.0360417800010037E-4	0.09439583865945145	3.936958764003814E-4
54-55	0.0011893759634411523	3.7090295724035935E-4	0.0	1.0360417800010037E-4	0.09732783689685429	3.936958764003814E-4
56-57	0.0012225293004011844	3.854075421603734E-4	0.0	1.0360417800010037E-4	0.10073848643661759	4.0612837776039345E-4
58-59	0.0012432501360012044	4.0820046132039546E-4	0.0	1.118925122401084E-4	0.1044537322597012	4.206329626804075E-4
60-61	0.0012971243085612566	4.185608791204055E-4	0.0	1.118925122401084E-4	0.10773176845162437	4.268492133604135E-4
62-63	0.0013302776455212887	4.227050462404095E-4	0.0	1.139645958001104E-4	0.11098493964082752	4.268492133604135E-4
64-65	0.0013551426482413128	4.289212969204155E-4	0.0	1.1810876292011443E-4	0.11511874634303153	4.268492133604135E-4
66-67	0.001369647233161327	4.558583832004416E-4	0.0	1.2018084648011643E-4	0.118705522985395	4.3099338048041753E-4
68-69	0.0014649630769214194	4.765792188004617E-4	0.0	1.2018084648011643E-4	0.12132256452167753	4.496421325204356E-4
70-71	0.0016120810096815618	4.8486755304046975E-4	0.0	1.2846918072012447E-4	0.12383600187995997	4.641467174404497E-4
72-73	0.001690820184961638	4.869396366004717E-4	0.0	1.3261334784012849E-4	0.12655043134356259	4.724350516804577E-4
74-75	0.001717757271241664	4.973000544004817E-4	0.0	1.409016820801365E-4	0.12947621333028542	4.745071352404597E-4
76-77	0.0017529826917616982	4.973000544004817E-4	0.0	1.4919001632014454E-4	0.13156280147520744	4.8072338592046573E-4
78-79	0.0017737035273617183	5.014442215204859E-4	0.0	1.8441543684017867E-4	0.1338545258925697	4.8072338592046573E-4
80-81	0.0018110010314417546	5.055883886404899E-4	0.0	1.906316875201847E-4	0.136233277819452	4.8072338592046573E-4
82-83	0.0018607310368818028	5.159488064404999E-4	0.0	1.906316875201847E-4	0.13850428140121418	4.8072338592046573E-4
84-85	0.0019208214601218608	5.180208900005019E-4	4.144167120004015E-6	1.906316875201847E-4	0.1405722207940962	4.8072338592046573E-4
86-87	0.0020202814710019576	5.304533913605139E-4	4.144167120004015E-6	1.906316875201847E-4	0.142439168081658	4.8072338592046573E-4
88-89	0.002092804395602028	5.34597558480518E-4	4.144167120004015E-6	1.9892002176019271E-4	0.1442998991185398	4.8072338592046573E-4
90-91	0.0021176693983220515	5.42885892720526E-4	4.144167120004015E-6	2.0306418888019673E-4	0.14680919230970224	4.8279546948046774E-4
92-93	0.002159111069522092	5.42885892720526E-4	4.144167120004015E-6	2.0306418888019673E-4	0.14933506216934467	4.890117201604737E-4
94-95	0.00223992232836217	5.42885892720526E-4	4.144167120004015E-6	2.0306418888019673E-4	0.15209922163838735	4.890117201604737E-4
96-97	0.0023414544228022686	5.4703005984053E-4	4.144167120004015E-6	2.0306418888019673E-4	0.15432878354894952	4.931558872804777E-4
98-99	0.002457491102162381	5.61534644760544E-4	4.144167120004015E-6	2.1135252312020475E-4	0.15624753292551136	4.973000544004817E-4
100-101	0.002571455697962491	5.63606728320546E-4	4.144167120004015E-6	2.1342460668020676E-4	0.15809997562815317	4.973000544004817E-4
102-103	0.0026066811184825255	5.63606728320546E-4	4.144167120004015E-6	2.1964085736021278E-4	0.15965196621459468	4.993721379604838E-4
104-105	0.00265226695680257	5.63606728320546E-4	4.144167120004015E-6	2.258571080402188E-4	0.16121431721883617	5.035163050804879E-4
106-107	0.0027413665498826558	5.63606728320546E-4	4.144167120004015E-6	2.3414544228022685E-4	0.16268756862999761	5.076604722004919E-4
108-109	0.0028366823936427483	5.63606728320546E-4	4.144167120004015E-6	2.4036169296023287E-4	0.16408622503299897	5.097325557604939E-4
110-111	0.0029382144880828465	5.6775089544055E-4	4.144167120004015E-6	2.4450586008023685E-4	0.1654455118483603	5.097325557604939E-4
112-113	0.003052179083882957	5.718950625605541E-4	4.144167120004015E-6	2.4450586008023685E-4	0.16707209744296186	5.097325557604939E-4
114-115	0.003143350760523045	5.843275639205661E-4	4.144167120004015E-6	2.548662778802469E-4	0.16880850346624354	5.097325557604939E-4
116-117	0.0032158736851231155	5.843275639205661E-4	4.144167120004015E-6	2.6108252856025296E-4	0.1705407653224052	5.097325557604939E-4
118-119	0.003278036191923176	5.967600652805781E-4	4.144167120004015E-6	2.69370862800261E-4	0.17194356589252657	5.118046393204959E-4
120-121	0.0034065053726433005	6.091925666405902E-4	4.144167120004015E-6	2.8594753128027703E-4	0.17322825769972783	5.138767228804979E-4
122-123	0.0035971370601634847	6.133367337605942E-4	4.144167120004015E-6	2.9009169840028105E-4	0.17451709367404905	5.138767228804979E-4
124-125	0.0036965970710435814	6.174809008805982E-4	4.144167120004015E-6	2.9009169840028105E-4	0.1757872808963303	5.180208900005019E-4
126-127	0.0037276783244436116	6.174809008805982E-4	4.144167120004015E-6	2.9009169840028105E-4	0.17709890978981158	5.200929735605039E-4
128-129	0.0037877687476836697	6.174809008805982E-4	4.144167120004015E-6	2.9216378196028305E-4	0.17839396201481283	5.242371406805079E-4
130-131	0.00387065209008375	6.216250680006022E-4	4.144167120004015E-6	2.9423586552028506E-4	0.17960820298097402	5.34597558480518E-4
132-133	0.003959751683163836	6.257692351206062E-4	4.144167120004015E-6	2.9630794908028706E-4	0.18080379519509515	5.34597558480518E-4
134-135	0.004127590451523999	6.257692351206062E-4	4.144167120004015E-6	2.9838003264028907E-4	0.18192479240105625	5.34597558480518E-4
136-137	0.004301645470564167	6.402738200406203E-4	4.144167120004015E-6	2.9838003264028907E-4	0.1830105641864973	5.3666964204052E-4
138-139	0.004326510473284192	6.796434076806584E-4	4.144167120004015E-6	2.9838003264028907E-4	0.18412327305821838	5.42885892720526E-4
>>END_MODULE
