##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.control.4d.R.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24130301
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	60-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.883729133755935	37.0	35.0	38.0	33.0	39.0
2	35.17456732097954	35.0	34.0	37.0	32.0	39.0
3	35.33645771762234	35.0	34.0	37.0	33.0	39.0
4	35.373501557232956	36.0	34.0	37.0	33.0	39.0
5	35.296039945792636	35.0	34.0	37.0	33.0	39.0
6	35.250302928256055	35.0	34.0	37.0	33.0	39.0
7	35.28686654178081	35.0	34.0	37.0	33.0	39.0
8	35.416309145915754	35.0	34.0	38.0	33.0	39.0
9	35.390352693901335	35.0	34.0	38.0	33.0	39.0
10-14	35.36382651007959	35.4	34.2	37.4	33.0	39.0
15-19	35.335676683021894	35.0	34.0	37.0	33.0	39.0
20-24	35.302348503651075	35.2	34.0	37.0	32.2	39.0
25-29	35.29248688609396	35.0	34.0	37.0	32.0	39.0
30-34	35.274315608412834	35.0	34.0	37.0	32.0	39.0
35-39	35.24989849069848	35.0	34.0	37.0	32.0	39.0
40-44	35.227642108567146	35.0	34.0	37.0	32.0	39.0
45-49	35.20143303641343	35.0	34.0	37.0	32.0	39.0
50-54	35.176434649530485	35.0	34.0	37.0	32.0	39.0
55-59	35.148070204345984	35.0	34.0	37.0	32.0	39.0
60-64	35.10697911231377	35.0	34.0	37.0	32.0	39.0
65-69	35.072566781058306	35.0	34.0	37.0	32.0	39.0
70-74	35.051988349118616	35.0	34.0	37.0	32.0	39.0
75-79	35.00235011441047	35.0	34.0	37.0	32.0	39.0
80-84	34.928083246341124	35.0	34.0	37.0	31.0	39.0
85-89	34.91160651604801	35.0	34.0	37.0	31.0	39.0
90-94	34.86102004957464	35.0	34.0	37.0	31.0	39.0
95-99	34.79840299759142	35.0	34.0	37.0	31.0	39.0
100-104	34.73483044173915	35.0	34.0	37.0	31.0	39.0
105-109	34.677418130935806	35.0	34.0	37.0	30.8	39.0
110-114	34.613177248068716	35.0	34.0	37.0	30.0	39.0
115-119	34.55675740330197	35.0	34.0	37.0	30.0	39.0
120-124	34.48349363233207	35.0	34.0	37.0	30.0	39.0
125-129	34.40526810546123	35.0	34.0	37.0	30.0	39.0
130-134	34.3300603181635	35.0	34.0	37.0	29.8	39.0
135-139	34.24291384741124	35.0	34.0	37.0	29.0	39.0
140-144	34.14839265692571	35.0	34.0	37.0	29.0	39.0
145-149	34.11737429790521	35.0	34.0	37.0	29.0	39.0
150	34.945599743596105	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	10.0
21	187.0
22	1971.0
23	10161.0
24	35068.0
25	86362.0
26	169914.0
27	287782.0
28	419155.0
29	550634.0
30	700505.0
31	903726.0
32	1195092.0
33	1642332.0
34	2429432.0
35	4077221.0
36	1.0116352E7
37	1504390.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.37019712269648	19.58134297620241	13.556826332170493	34.49163356893061
2	23.818725834324482	26.5884344579222	33.72846172654298	15.864377981210346
3	18.66227445733064	30.41876684422627	29.484998964579844	21.433959733863244
4	22.329796051860274	34.92048441500999	22.78157243044751	19.968147102682224
5	24.323811791655643	35.52013296477321	23.028635241640792	17.127420001930354
6	18.397702540055345	38.49673072872154	23.981213495844912	19.12435323537821
7	19.60064650664739	20.699878546894215	38.514161095628275	21.185313850830124
8	20.833835468270184	23.264082916499174	29.154486268301678	26.747595346928964
9	22.949431919643274	23.343923476130694	29.39766893086	24.30897567336603
10-14	22.919971415523932	29.080754178420804	25.859450752138578	22.139823653916686
15-19	22.73407070610633	28.330183338039312	27.268836427176545	21.666909528677817
20-24	22.855249959503304	28.320227062060336	27.19120703434864	21.633315944087713
25-29	22.929024383077525	28.366834711261994	27.120928164136863	21.583212741523614
30-34	23.010653700507092	28.105477838838393	27.23158488574179	21.65228357491272
35-39	23.078861718301816	28.094558787310607	27.237007114001603	21.58957238038597
40-44	23.261715632971175	27.80724533854758	27.370562016611395	21.560477011869846
45-49	23.276971140973334	27.656905730268345	27.399328338258194	21.666794790500127
50-54	23.375531232499185	27.607563018858016	27.365798108907118	21.65110763973568
55-59	23.527904604256697	27.50903770325948	27.255543973529385	21.707513718954438
60-64	23.46237712626456	27.469033620602602	27.302429801616384	21.766159451516458
65-69	23.567697541228508	27.37551018026535	27.255443679957754	21.801348598548387
70-74	23.616611012528203	27.35290484184581	27.15668582558156	21.87379832004443
75-79	23.562232188306528	27.395771606213508	27.216363854198296	21.825632351281666
80-84	23.67131769352551	27.435453901100587	27.100349282727503	21.7928791226464
85-89	23.720699644834536	27.352243067158653	27.131788477775476	21.795268810231335
90-94	23.64348368226805	27.285448082837238	27.2021486770894	21.86891955780531
95-99	23.74634462844284	27.422713669268205	27.136143977440447	21.694797724848502
100-104	23.912418509550747	27.412101549292462	26.938661227504856	21.736818713651935
105-109	23.69524831466567	27.54531771255274	27.03692428120207	21.722509691579525
110-114	23.73367043711839	27.562482542324602	26.977206022278615	21.72664099827839
115-119	23.814162987983273	27.54599053295921	26.981235349286536	21.65861112977098
120-124	23.780125229236305	27.462454849774513	27.163436863526357	21.593983057462825
125-129	23.770122020615588	27.522961964684185	27.121762730690786	21.585153284009444
130-134	23.83139876348968	27.44378996427291	27.195896808221935	21.528914464015475
135-139	23.749187681645843	27.4691416446828	27.294768932215185	21.48690174145617
140-144	23.781894690589084	27.59136012247557	27.25922905205145	21.3675161348839
145-149	23.549984869626794	27.64898425105618	27.219775566737724	21.5812553125793
150	23.646353551621768	26.473016816024625	27.67482839446254	22.205801237891063
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	8.0
3	27.5
4	35.0
5	27.0
6	23.5
7	21.0
8	14.0
9	9.5
10	7.0
11	7.5
12	9.0
13	11.5
14	22.0
15	39.0
16	75.0
17	153.0
18	260.0
19	433.5
20	649.0
21	1008.0
22	1569.5
23	2578.0
24	4380.0
25	7254.0
26	11536.0
27	18298.0
28	30031.0
29	46165.5
30	61670.5
31	81026.5
32	107524.5
33	140893.5
34	189820.0
35	261680.0
36	363933.5
37	490656.5
38	640161.5
39	826102.5
40	1036959.0
41	1230895.5
42	1412498.5
43	1583297.0
44	1698078.5
45	1745964.5
46	1743974.0
47	1686669.5
48	1573552.0
49	1424481.0
50	1239116.5
51	1036108.5
52	842385.0
53	667758.5
54	518096.0
55	390306.0
56	290097.0
57	214982.0
58	155695.0
59	111183.5
60	75802.5
61	49978.0
62	33920.5
63	23809.0
64	16547.0
65	11201.0
66	8095.0
67	6291.5
68	5619.0
69	3984.0
70	1842.5
71	1101.0
72	606.5
73	478.0
74	407.0
75	263.5
76	170.0
77	61.5
78	17.0
79	2.5
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.144167120004015E-6
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	4.144167120004015E-6
9	0.0
10-14	1.657666848001606E-6
15-19	1.657666848001606E-6
20-24	0.03044056516327749
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.28833424000803E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	2.4865022710717146E-6
75-79	7.078257739737088E-4
80-84	0.05848116463567605
85-89	1.657684145956901E-6
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	3.7910952862516684E-4
145-149	0.03157110011128114
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
60-61	2.0
62-63	2.0
64-65	2.0
66-67	1.0
68-69	2.0
70-71	9.0
72-73	14.0
74-75	23.0
76-77	29.0
78-79	22.0
80-81	30.0
82-83	42.0
84-85	43.0
86-87	54.0
88-89	113.0
90-91	86.0
92-93	97.0
94-95	111.0
96-97	126.0
98-99	132.0
100-101	152.0
102-103	153.0
104-105	140.0
106-107	160.0
108-109	195.0
110-111	216.0
112-113	177.0
114-115	217.0
116-117	235.0
118-119	250.0
120-121	275.0
122-123	332.0
124-125	357.0
126-127	453.0
128-129	530.0
130-131	665.0
132-133	784.0
134-135	1124.0
136-137	1642.0
138-139	3534.0
140-141	7605.0
142-143	12154.0
144-145	73985.0
146-147	849359.0
148-149	1134440.0
150-151	2.2040227E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.91230072417283
#Duplication Level	Percentage of total
1	28.89751714559845
2	10.242064327572677
3	6.9138573491050686
4	5.035260864053573
5	3.7598970902797726
6	2.958458171321892
7	2.508987866915282
8	2.114507232120818
9	1.8020631209504576
>10	20.811418264778624
>50	5.423428161986322
>100	6.859731553123993
>500	1.3506594018418645
>1k	1.153483037160289
>5k	0.11954825664816267
>10k+	0.04911815654255645
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.2432501360012045E-5	0.0	0.0	0.0013137009770412727	0.0
2	1.2432501360012045E-5	3.315333696003212E-5	0.0	0.0	0.006435891537366235	0.0
3	2.9009169840028105E-5	3.315333696003212E-5	0.0	0.0	0.00835049674680809	0.0
4	8.70275095200843E-5	3.315333696003212E-5	0.0	0.0	0.010082758602969769	0.0
5	8.70275095200843E-5	3.315333696003212E-5	0.0	0.0	0.011972498809691598	0.0
6	9.946001088009636E-5	3.315333696003212E-5	4.144167120004015E-6	4.144167120004015E-6	0.01382079734521339	0.0
7	1.0774834512010438E-4	4.1441671200040147E-5	4.144167120004015E-6	4.144167120004015E-6	0.01605035925577555	0.0
8	1.8234335328017666E-4	4.1441671200040147E-5	4.144167120004015E-6	8.28833424000803E-6	0.022212735763221518	0.0
9	1.947758546401887E-4	4.5585838320044166E-5	4.144167120004015E-6	8.28833424000803E-6	0.028308805596747425	0.0
10-11	2.2171294092021478E-4	5.1802089000050185E-5	6.216250680006022E-6	8.28833424000803E-6	0.03510938384067401	0.0
12-13	2.5693836144024895E-4	6.216250680006023E-5	8.28833424000803E-6	8.28833424000803E-6	0.04179806957236049	0.0
14-15	2.9423586552028506E-4	6.630667392006424E-5	8.28833424000803E-6	8.28833424000803E-6	0.05088208389940929	0.0
16-17	3.1910086824030913E-4	6.630667392006424E-5	8.28833424000803E-6	8.28833424000803E-6	0.0565658091044948	0.0
18-19	3.957679599603834E-4	7.045084104006826E-5	8.28833424000803E-6	8.28833424000803E-6	0.06437549204214237	0.0
20-21	4.6829088456045364E-4	7.459500816007227E-5	8.28833424000803E-6	8.28833424000803E-6	0.0708030952452686	0.0
22-23	5.283813078005119E-4	8.70275095200843E-5	8.28833424000803E-6	8.28833424000803E-6	0.07769070099871526	0.0
24-25	6.071204830805882E-4	9.117167664008833E-5	8.28833424000803E-6	8.28833424000803E-6	0.0832024432683206	0.0
26-27	6.609946556406403E-4	9.531584376009234E-5	8.28833424000803E-6	8.28833424000803E-6	0.08775481084964501	0.0
28-29	7.625267500807388E-4	9.531584376009234E-5	8.28833424000803E-6	8.28833424000803E-6	0.0926884418060098	0.0
30-31	9.552305211609255E-4	9.946001088009636E-5	8.28833424000803E-6	8.28833424000803E-6	0.09775054194309471	0.0
32-33	0.0011790155456411423	1.118925122401084E-4	8.28833424000803E-6	8.28833424000803E-6	0.10282300249797961	0.0
34-35	0.0013344218126412927	1.1603667936011242E-4	8.28833424000803E-6	8.28833424000803E-6	0.10634347246642303	0.0
36-37	0.0013737914002813307	1.1603667936011242E-4	8.28833424000803E-6	8.28833424000803E-6	0.11020169205514677	0.0
38-39	0.0014214493221613772	1.2225293004011843E-4	8.28833424000803E-6	8.28833424000803E-6	0.1137511711934302	0.0
40-41	0.0014628909933614173	1.346854314001305E-4	8.28833424000803E-6	8.28833424000803E-6	0.11682614319647319	4.144167120004015E-6
42-43	0.0015022605810014553	1.388295985201345E-4	8.28833424000803E-6	8.28833424000803E-6	0.11966489767367594	4.144167120004015E-6
44-45	0.0015478464193214997	1.5747835056015256E-4	8.28833424000803E-6	8.28833424000803E-6	0.12216175836347834	4.144167120004015E-6
46-47	0.0015830718398415337	2.1549669024020876E-4	8.28833424000803E-6	8.28833424000803E-6	0.12498393617220108	4.144167120004015E-6
48-49	0.0016286576781615778	2.1964085736021278E-4	1.2432501360012045E-5	8.28833424000803E-6	0.128009178169804	4.144167120004015E-6
50-51	0.00167217143292162	2.279291916002208E-4	1.657666848001606E-5	8.28833424000803E-6	0.13039414634736635	4.144167120004015E-6
52-53	0.0017239735219216701	2.3828960940023086E-4	1.657666848001606E-5	8.28833424000803E-6	0.1327563216057686	4.144167120004015E-6
54-55	0.0018192893656817626	2.5693836144024895E-4	1.657666848001606E-5	8.28833424000803E-6	0.1349340814273307	4.144167120004015E-6
56-57	0.001914605209441855	2.67298779240259E-4	1.657666848001606E-5	1.2432501360012045E-5	0.13715742708721287	4.144167120004015E-6
58-59	0.0020078489696419453	2.81803364160273E-4	1.657666848001606E-5	1.2432501360012045E-5	0.1392398710650149	4.144167120004015E-6
60-61	0.002109381064082044	2.9009169840028105E-4	1.657666848001606E-5	1.4504584920014053E-5	0.14076492456517636	4.144167120004015E-6
62-63	0.0021611831530820936	2.9838003264028907E-4	1.657666848001606E-5	1.657666848001606E-5	0.14280178270465835	4.144167120004015E-6
64-65	0.002186048155802118	3.149567011203051E-4	1.657666848001606E-5	1.657666848001606E-5	0.14457963039914007	4.144167120004015E-6
66-67	0.002300012751602228	3.605425394403493E-4	1.657666848001606E-5	1.657666848001606E-5	0.14609846764862155	4.144167120004015E-6
68-69	0.0023310940050022583	3.7711920792036537E-4	1.657666848001606E-5	1.657666848001606E-5	0.14738730362294278	4.144167120004015E-6
70-71	0.002370463592642296	3.833354586003714E-4	1.657666848001606E-5	1.657666848001606E-5	0.14879424836018415	4.144167120004015E-6
72-73	0.0024015448460423267	3.9991212708038743E-4	1.657666848001606E-5	1.657666848001606E-5	0.14973083012930505	4.144167120004015E-6
74-75	0.002426409848762351	4.0820046132039546E-4	1.657666848001606E-5	1.8648752040018067E-5	0.15094921526258623	4.144167120004015E-6
76-77	0.002457491102162381	4.268492133604135E-4	1.657666848001606E-5	2.0720835600020073E-5	0.15230642999438754	4.144167120004015E-6
78-79	0.002523797776082445	4.5585838320044166E-4	1.657666848001606E-5	2.0720835600020073E-5	0.15409049393954927	4.144167120004015E-6
80-81	0.002538302361002459	4.724350516804577E-4	1.657666848001606E-5	2.0720835600020073E-5	0.15557824993563074	4.144167120004015E-6
82-83	0.002554879029482475	4.993721379604838E-4	1.657666848001606E-5	2.0720835600020073E-5	0.1571861867781923	4.144167120004015E-6
84-85	0.0025755998650824953	5.055883886404899E-4	1.657666848001606E-5	2.0720835600020073E-5	0.15891430446723398	8.28833424000803E-6
86-87	0.0026170415362825354	5.221650571205059E-4	2.0720835600020073E-5	2.0720835600020073E-5	0.16079161217259577	8.28833424000803E-6
88-89	0.002631546121202549	5.34597558480518E-4	2.0720835600020073E-5	2.0720835600020073E-5	0.16265648737659757	8.28833424000803E-6
90-91	0.00265226695680257	5.63606728320546E-4	2.0720835600020073E-5	2.0720835600020073E-5	0.16363243873335853	8.28833424000803E-6
92-93	0.0026564111239225736	5.63606728320546E-4	2.0720835600020073E-5	2.0720835600020073E-5	0.1646456875941995	8.28833424000803E-6
94-95	0.0026646994581625816	5.6775089544055E-4	2.0720835600020073E-5	2.0720835600020073E-5	0.1655781251962004	1.2432501360012045E-5
96-97	0.0026646994581625816	5.905438146005721E-4	2.0720835600020073E-5	2.0720835600020073E-5	0.16655407655296137	1.657666848001606E-5
98-99	0.0026709157088425873	6.071204830805882E-4	2.0720835600020073E-5	2.0720835600020073E-5	0.16746786540292224	1.657666848001606E-5
100-101	0.0026729877924025897	6.154088173205962E-4	2.0720835600020073E-5	2.0720835600020073E-5	0.16828426632556304	2.0720835600020073E-5
102-103	0.0026729877924025897	6.216250680006022E-4	2.0720835600020073E-5	2.0720835600020073E-5	0.16911517183312383	2.0720835600020073E-5
104-105	0.0026771319595225935	6.257692351206062E-4	2.0720835600020073E-5	2.0720835600020073E-5	0.17002481651596474	2.2792919160022083E-5
106-107	0.0027061411293626218	6.382017364806183E-4	2.486500272002409E-5	2.0720835600020073E-5	0.17079977576740546	2.486500272002409E-5
108-109	0.002718573630722634	6.382017364806183E-4	2.486500272002409E-5	2.0720835600020073E-5	0.17152914918052617	2.486500272002409E-5
110-111	0.002731006132082646	6.423459036006223E-4	2.486500272002409E-5	2.0720835600020073E-5	0.17231446884976692	2.486500272002409E-5
112-113	0.0027413665498826558	6.444179871606243E-4	2.486500272002409E-5	2.486500272002409E-5	0.17305420268068766	2.9009169840028105E-5
114-115	0.0027434386334426577	6.506342378406303E-4	2.486500272002409E-5	2.486500272002409E-5	0.17398664028268856	3.1081253400030115E-5
116-117	0.0027496548841226638	6.589225720806383E-4	2.486500272002409E-5	2.9009169840028105E-5	0.1748797082970494	3.7297504080036134E-5
118-119	0.00275587113480267	6.609946556406403E-4	2.9009169840028105E-5	3.1081253400030115E-5	0.17564223504713017	4.1441671200040147E-5
120-121	0.00275587113480267	6.713550734406504E-4	2.9009169840028105E-5	3.315333696003212E-5	0.17643377096705093	4.1441671200040147E-5
122-123	0.0027600153019226737	6.713550734406504E-4	2.9009169840028105E-5	3.315333696003212E-5	0.17721080230205166	4.1441671200040147E-5
124-125	0.002772447803282686	6.734271570006524E-4	2.9009169840028105E-5	3.315333696003212E-5	0.1780707169794525	4.765792188004617E-5
126-127	0.002789024471762702	6.837875748006624E-4	3.1081253400030115E-5	3.315333696003212E-5	0.17909432625809352	4.973000544004818E-5
128-129	0.0028076732238027204	6.920759090406704E-4	3.315333696003212E-5	3.315333696003212E-5	0.18010550303537448	4.973000544004818E-5
130-131	0.0028304661429627426	7.086525775206866E-4	3.315333696003212E-5	3.315333696003212E-5	0.18097163396345534	5.1802089000050185E-5
132-133	0.002855331145682766	7.086525775206866E-4	3.522542052003413E-5	3.315333696003212E-5	0.1817838907189761	7.459500816007227E-5
134-135	0.0028801961484027903	7.086525775206866E-4	3.7297504080036134E-5	3.7297504080036134E-5	0.18260443580873692	8.288334240008029E-5
136-137	0.0028843403155227945	7.190129953206966E-4	4.1441671200040147E-5	3.7297504080036134E-5	0.18333795338897763	8.70275095200843E-5
138-139	0.0028884844826427983	7.210850788806986E-4	4.1441671200040147E-5	3.7297504080036134E-5	0.18400723637885827	8.70275095200843E-5
>>END_MODULE
