##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.control.4d.S.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24211874
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	61-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.96721926605103	37.0	35.0	38.0	33.0	39.0
2	35.332572687269064	35.0	34.0	37.0	33.0	39.0
3	35.459170157584666	35.0	34.0	37.0	33.0	39.0
4	35.51996747546266	36.0	35.0	38.0	33.0	39.0
5	35.43759157180481	36.0	35.0	37.0	33.0	39.0
6	35.38908830435843	35.0	35.0	37.0	33.0	39.0
7	35.42739203086882	35.0	34.0	38.0	33.0	39.0
8	35.518505093822974	36.0	35.0	38.0	33.0	39.0
9	35.501874906502486	36.0	35.0	38.0	33.0	39.0
10-14	35.48011234487673	35.8	34.4	37.8	33.0	39.0
15-19	35.457696690475096	36.0	34.0	38.0	33.0	39.0
20-24	35.44496711819993	36.0	34.0	38.0	33.0	39.0
25-29	35.42880845984908	36.0	34.0	38.0	33.0	39.0
30-34	35.41007562652936	36.0	34.0	38.0	33.0	39.0
35-39	35.38140170397385	36.0	34.0	38.0	33.0	39.0
40-44	35.365050611117496	36.0	34.0	38.0	32.8	39.0
45-49	35.33602788449998	36.0	34.0	38.0	32.0	39.0
50-54	35.31429593595275	36.0	34.0	38.0	32.0	39.0
55-59	35.288631768032495	36.0	34.0	38.0	32.0	39.0
60-64	35.24507038751497	36.0	34.0	38.0	32.0	39.0
65-69	35.215782885457955	36.0	34.0	38.0	32.0	39.0
70-74	35.18364792484448	36.0	34.0	38.0	32.0	39.0
75-79	35.13276680930237	36.0	34.0	38.0	32.0	39.0
80-84	35.08861196237623	35.6	34.0	38.0	32.0	39.0
85-89	35.03879238836092	35.0	34.0	37.4	32.0	39.0
90-94	34.98400155515079	35.0	34.0	37.0	31.4	39.0
95-99	34.9177478471589	35.0	34.0	37.0	31.0	39.0
100-104	34.86832858621629	35.0	34.0	37.0	31.0	39.0
105-109	34.80621650325679	35.0	34.0	37.0	31.0	39.0
110-114	34.73084583293418	35.0	34.0	37.0	31.0	39.0
115-119	34.658214398657194	35.0	34.0	37.0	30.0	39.0
120-124	34.58324690675768	35.0	34.0	37.0	30.0	39.0
125-129	34.50361132869453	35.0	34.0	37.0	30.0	39.0
130-134	34.398822927621566	35.0	34.0	37.0	29.8	39.0
135-139	34.34192096859453	35.0	34.0	37.0	29.8	39.0
140-144	34.220485261838434	35.0	34.0	37.0	29.0	39.0
145-149	34.20758121789845	35.0	34.0	37.0	29.0	39.0
150	35.010430400632885	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	154.0
22	1511.0
23	8400.0
24	29912.0
25	75130.0
26	150771.0
27	258921.0
28	382103.0
29	506109.0
30	652109.0
31	852405.0
32	1142991.0
33	1594836.0
34	2385604.0
35	4024577.0
36	1.0056143E7
37	2090185.0
38	6.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.27228615182782	18.966012296280745	15.423172117945105	32.338529433946334
2	24.32427163630539	25.632191874119286	32.42816727032364	17.61536921925168
3	20.045428949448524	29.22373542832744	29.482249081586993	21.248586540637042
4	23.73043903995205	33.824226080145635	21.635974976575543	20.809359903326772
5	25.506526260627325	34.84198703495648	21.726409942493504	17.925076761922686
6	19.235673372494837	38.83893084855802	22.049243276253627	19.876152502693515
7	20.582082989528196	20.87117254946891	36.675570837680716	21.871173623322175
8	21.612251079780112	23.542725358640144	28.054499209767904	26.79052435181184
9	23.542254515284526	23.501225060067636	27.79455237541712	25.161968049230722
10-14	23.119267017497283	28.877755600413252	25.21144955570147	22.791527826387995
15-19	23.152501124035258	27.76323220581769	26.721478890894605	22.362787779252443
20-24	23.067578329541945	27.74042604054523	26.647056729272588	22.54493890064024
25-29	23.14605965651399	27.371579746367424	26.633005772291728	22.849354824826857
30-34	23.00008703167854	27.097572490218187	26.852996622285357	23.049343855817916
35-39	23.295946443468193	26.842187432496967	26.921328766207854	22.94053735782699
40-44	23.447583611247936	26.502830801118492	26.949277036548263	23.100308551085305
45-49	23.368802431402045	26.346566977839053	27.00841413597312	23.276216454785782
50-54	23.576293185731924	26.363846102949324	26.73595691105943	23.323903800259327
55-59	23.613343796629238	26.367977351674448	26.7517097334284	23.266969118267912
60-64	23.32628089819766	26.33428522718114	26.82276622179926	23.51666765282194
65-69	23.540215458772266	26.263235914316574	26.840414770093563	23.356133856817593
70-74	23.680680178217735	26.239400744747215	26.585656581895368	23.494262495139676
75-79	23.491476818873664	26.345551757048575	26.829719593527994	23.333251830549763
80-84	23.810796309068145	26.340948612560037	26.524434235728016	23.3238208426438
85-89	23.536851661648843	26.437770992881717	26.755334130670644	23.270043214798804
90-94	23.55304579563636	26.164521956486116	26.806359322744804	23.47607292513272
95-99	23.56789101690345	26.31505272130831	26.822491333237164	23.29456492855108
100-104	23.787562975274433	26.152021502530083	26.765675584018776	23.294739938176708
105-109	23.589674602406884	26.32685527414787	26.67997573670676	23.403494386738487
110-114	23.688530855500957	26.41186957566945	26.57883340644272	23.32076616238687
115-119	23.83259501940968	26.482940782788578	26.71793373600696	22.96653046179478
120-124	23.774252628145454	26.644789914329436	26.829627389215442	22.751330068309667
125-129	23.765206417522524	26.70401442094743	26.67289294465781	22.857886216872238
130-134	23.922074315783696	26.717771256698796	26.666194518440093	22.69395990907741
135-139	23.704580202487506	26.755087459044173	26.930143038362274	22.610189300106043
140-144	23.735013942871593	26.849632105550203	26.72832396378888	22.687029987789323
145-149	23.53564960292744	27.09847334664567	26.572493133709262	22.79338391671763
150	23.661445854571742	25.96389937657112	26.865823815000294	23.50883095385684
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.0
9	3.0
10	3.0
11	3.0
12	5.0
13	11.5
14	16.5
15	26.0
16	50.0
17	90.0
18	173.5
19	293.0
20	473.0
21	681.5
22	1013.0
23	1715.5
24	2800.0
25	4539.0
26	7269.0
27	11533.0
28	17461.5
29	24609.0
30	34721.0
31	49751.5
32	71411.5
33	98356.0
34	133926.5
35	189741.5
36	267263.5
37	361333.0
38	479186.0
39	625390.0
40	800308.0
41	989539.0
42	1181806.0
43	1371863.5
44	1520894.5
45	1617886.0
46	1646950.0
47	1610181.5
48	1560944.5
49	1506242.0
50	1399139.0
51	1245133.5
52	1058860.0
53	880557.5
54	740955.0
55	627669.5
56	516341.0
57	407444.0
58	332605.0
59	270566.5
60	202101.5
61	135444.0
62	84484.5
63	51569.5
64	31450.5
65	15992.5
66	8875.0
67	4962.5
68	2885.0
69	1765.0
70	863.5
71	609.5
72	389.5
73	276.0
74	233.0
75	145.5
76	92.5
77	36.5
78	10.0
79	3.0
80	1.5
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	1.6520819495426088E-6
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	8.260409747713044E-7
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	4.130694035742102E-6
130-134	0.04964678795518296
135-139	8.262678139725771E-7
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
60-61	1.0
62-63	0.0
64-65	1.0
66-67	2.0
68-69	1.0
70-71	3.0
72-73	3.0
74-75	5.0
76-77	9.0
78-79	21.0
80-81	12.0
82-83	15.0
84-85	30.0
86-87	35.0
88-89	48.0
90-91	36.0
92-93	46.0
94-95	46.0
96-97	80.0
98-99	70.0
100-101	76.0
102-103	106.0
104-105	117.0
106-107	103.0
108-109	104.0
110-111	152.0
112-113	152.0
114-115	162.0
116-117	182.0
118-119	201.0
120-121	234.0
122-123	297.0
124-125	311.0
126-127	395.0
128-129	419.0
130-131	547.0
132-133	708.0
134-135	981.0
136-137	1611.0
138-139	4361.0
140-141	8569.0
142-143	10018.0
144-145	62586.0
146-147	796281.0
148-149	1077858.0
150-151	2.2244879E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.49192441210675
#Duplication Level	Percentage of total
1	25.962624783698363
2	8.477588662008385
3	5.187220578447492
4	3.72488131066815
5	2.8746753684420128
6	2.233685896640881
7	1.8527889593574511
8	1.5738557309144081
9	1.3822975493723884
>10	16.991584683769887
>50	6.089861022044717
>100	12.8185751483025
>500	3.957877693239188
>1k	5.404286317247538
>5k	0.878279544927699
>10k+	0.5899167509188866
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	28154	0.11628178801855651	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.551976191516609E-4	0.0	0.0	0.0	0.001899894241974	0.0
2	0.0014703529350929218	4.1302048738565215E-6	0.0	0.0	0.009173185024835336	0.0
3	0.0022055294026393827	4.1302048738565215E-6	0.0	4.1302048738565215E-6	0.013662717722717373	0.0
4	0.005303183058031774	4.1302048738565215E-6	0.0	4.1302048738565215E-6	0.02003562384307799	0.0
5	0.0055344745309677396	4.1302048738565215E-6	0.0	4.1302048738565215E-6	0.024099745438952803	0.0
6	0.006542244520188731	4.1302048738565215E-6	0.0	4.1302048738565215E-6	0.031042619831905618	0.0
7	0.008661039620477127	8.260409747713043E-6	0.0	4.1302048738565215E-6	0.04181006393804957	0.0
8	0.01502155512621617	8.260409747713043E-6	0.0	4.1302048738565215E-6	0.05710008238106641	0.0
9	0.015562611964691374	8.260409747713043E-6	0.0	4.1302048738565215E-6	0.06823098451610975	0.0
10-11	0.016252356178625416	1.4455717058497826E-5	0.0	4.1302048738565215E-6	0.11264307752468891	0.0
12-13	0.0170040534656673	1.6520819495426086E-5	0.0	4.1302048738565215E-6	0.13393841385429314	0.0
14-15	0.020087251404001194	2.4781229243139132E-5	0.0	4.1302048738565215E-6	0.14394383516121056	0.0
16-17	0.02350086573224361	2.684633168006739E-5	0.0	8.260409747713043E-6	0.15170862032406082	0.0
18-19	0.029968766564702924	3.0976536553923914E-5	0.0	8.260409747713043E-6	0.16004130865706637	0.0
20-21	0.035269884520297765	3.7171843864708695E-5	0.0	8.260409747713043E-6	0.1653279708956027	0.0
22-23	0.03736596349377995	4.130204873856522E-5	0.0	8.260409747713043E-6	0.1708954870655613	0.0
24-25	0.042411008747195694	4.543225361242174E-5	0.0	8.260409747713043E-6	0.17604791764569733	0.0
26-27	0.046945973698690156	4.9562458486278264E-5	0.0	8.260409747713043E-6	0.18013682047081528	0.0
28-29	0.05851261244792535	5.7822868233991304E-5	0.0	8.260409747713043E-6	0.18437854087626593	0.0
30-31	0.08161284830740487	7.434368772941739E-5	0.0	8.260409747713043E-6	0.18834147245273125	0.0
32-33	0.0997299919865765	8.46691999140587E-5	0.0	8.260409747713043E-6	0.19124707158148932	0.0
34-35	0.11269057488073828	8.879940478791521E-5	0.0	8.260409747713043E-6	0.19428277216377388	0.0
36-37	0.11522239046841232	9.086450722484348E-5	0.0	8.260409747713043E-6	0.19621983824961256	0.0
38-39	0.11749400314903341	9.086450722484348E-5	0.0	8.260409747713043E-6	0.19793387327226303	0.0
40-41	0.11879708278673515	9.086450722484348E-5	0.0	8.260409747713043E-6	0.19968301503634128	0.0
42-43	0.11908826223034202	9.912491697255653E-5	0.0	8.260409747713043E-6	0.20143835210773028	0.0
44-45	0.11991636830755026	1.0325512184641305E-4	0.0	8.260409747713043E-6	0.20296239770618335	0.0
46-47	0.1212277083549997	1.1977594134183914E-4	0.0	8.260409747713043E-6	0.20446785738270404	0.0
48-49	0.12240068653917495	1.2390614621569565E-4	0.0	8.260409747713043E-6	0.2057791974301535	0.0
50-51	0.12366452923057505	1.2390614621569565E-4	0.0	8.260409747713043E-6	0.20715868585802155	0.0
52-53	0.12490772089760585	1.259712486526239E-4	0.0	8.260409747713043E-6	0.20848035141765564	0.0
54-55	0.1254591032482657	1.2803635108955217E-4	4.1302048738565215E-6	8.260409747713043E-6	0.20960583224578155	0.0
56-57	0.12627894891572622	1.321665559634087E-4	4.1302048738565215E-6	8.260409747713043E-6	0.21078913594214144	0.0
58-59	0.126458612827739	1.4455717058497826E-4	6.195307310784782E-6	8.260409747713043E-6	0.21176179918993465	0.0
60-61	0.12678283391033673	1.4662227302190652E-4	8.260409747713043E-6	8.260409747713043E-6	0.21261881670125987	4.1302048738565215E-6
62-63	0.1271091200953714	1.4868737545883478E-4	8.260409747713043E-6	8.260409747713043E-6	0.21364723771485017	4.1302048738565215E-6
64-65	0.1271690080660423	1.5075247789576304E-4	8.260409747713043E-6	8.260409747713043E-6	0.21457446870903096	4.1302048738565215E-6
66-67	0.12723302624158708	1.590128876434761E-4	8.260409747713043E-6	8.260409747713043E-6	0.21524149679615878	4.1302048738565215E-6
68-69	0.12728878400738414	1.6520819495426087E-4	8.260409747713043E-6	8.260409747713043E-6	0.21594982693202516	4.1302048738565215E-6
70-71	0.12741888546091062	1.6520819495426087E-4	8.260409747713043E-6	8.260409747713043E-6	0.21667467788738698	8.260409747713043E-6
72-73	0.12747877343158154	1.734686047019739E-4	8.260409747713043E-6	8.260409747713043E-6	0.2173313804623302	1.2390614621569566E-5
74-75	0.12750974996813547	1.734686047019739E-4	8.260409747713043E-6	8.260409747713043E-6	0.2180417757006335	1.2390614621569566E-5
76-77	0.12753659629981554	1.755337071389022E-4	8.260409747713043E-6	8.260409747713043E-6	0.21893183485094958	1.2390614621569566E-5
78-79	0.1276192003972927	1.7759880957583045E-4	8.260409747713043E-6	1.2390614621569566E-5	0.2200882922156294	1.2390614621569566E-5
80-81	0.12769147898258515	1.8172901444968697E-4	8.260409747713043E-6	1.2390614621569566E-5	0.22100932790249941	1.2390614621569566E-5
82-83	0.12774723674838223	1.8172901444968697E-4	1.0325512184641305E-5	1.4455717058497826E-5	0.22189112664306776	1.4455717058497826E-5
84-85	0.12779886430930543	1.8172901444968697E-4	1.2390614621569566E-5	2.065102436928261E-5	0.2227047770032175	1.6520819495426086E-5
86-87	0.127840166358044	1.8172901444968697E-4	1.2390614621569566E-5	2.065102436928261E-5	0.22362168248521366	1.6520819495426086E-5
88-89	0.1278835335092195	1.8172901444968697E-4	1.2390614621569566E-5	2.065102436928261E-5	0.2244497885624219	2.065102436928261E-5
90-91	0.12791657514821034	1.8172901444968697E-4	1.4455717058497826E-5	2.065102436928261E-5	0.22504660316669417	2.065102436928261E-5
92-93	0.12793309596770577	1.8172901444968697E-4	1.8585921932354347E-5	2.065102436928261E-5	0.22563928756609258	2.065102436928261E-5
94-95	0.12798678863106588	1.8172901444968697E-4	2.065102436928261E-5	2.065102436928261E-5	0.22639098485313447	2.4781229243139132E-5
96-97	0.12799917924568746	1.9411962907125651E-4	2.065102436928261E-5	2.065102436928261E-5	0.2270435572232038	2.4781229243139132E-5
98-99	0.12801983027005676	1.9618473150818477E-4	2.065102436928261E-5	2.065102436928261E-5	0.22771058531033161	3.304163899085217E-5
100-101	0.12805493701148452	2.0238003881896957E-4	2.065102436928261E-5	2.065102436928261E-5	0.2282722931731761	3.7171843864708695E-5
102-103	0.1280755880358538	2.0238003881896957E-4	2.4781229243139132E-5	2.065102436928261E-5	0.22883193593358367	3.7171843864708695E-5
104-105	0.12811689008459237	2.0238003881896957E-4	2.8911434116995652E-5	2.065102436928261E-5	0.22943701094760366	3.7171843864708695E-5
106-107	0.1281251504943401	2.0444514125589783E-4	2.8911434116995652E-5	2.065102436928261E-5	0.23000284901532198	3.7171843864708695E-5
108-109	0.12816025723576788	2.1270555100361087E-4	2.8911434116995652E-5	2.065102436928261E-5	0.2305521662635449	3.7171843864708695E-5
110-111	0.1282284056161865	2.2096596075132393E-4	2.8911434116995652E-5	2.065102436928261E-5	0.2310994184093309	3.7171843864708695E-5
112-113	0.1282490566405558	2.271612680621087E-4	2.8911434116995652E-5	2.065102436928261E-5	0.2316549309648646	3.7171843864708695E-5
114-115	0.1282655774600512	2.271612680621087E-4	2.8911434116995652E-5	2.065102436928261E-5	0.2321939227009029	3.7171843864708695E-5
116-117	0.1282800331771097	2.2922637049903696E-4	2.8911434116995652E-5	2.065102436928261E-5	0.23271639361744573	3.7171843864708695E-5
118-119	0.12828829358685742	2.3129147293596522E-4	3.304163899085217E-5	2.065102436928261E-5	0.23324505984129934	3.7171843864708695E-5
120-121	0.12829448889416822	2.3129147293596522E-4	3.304163899085217E-5	2.065102436928261E-5	0.23387491608456248	3.7171843864708695E-5
122-123	0.12831100971366366	2.3542167780982173E-4	3.304163899085217E-5	2.065102436928261E-5	0.2344324937425331	3.7171843864708695E-5
124-125	0.12831927012341135	2.3542167780982173E-4	3.304163899085217E-5	2.065102436928261E-5	0.23499213650294065	3.7171843864708695E-5
126-127	0.12833166073803293	2.3542167780982173E-4	3.304163899085217E-5	2.271612680621087E-5	0.23564883907788386	4.130204873856522E-5
128-129	0.12834611645509142	2.3748678024675E-4	3.304163899085217E-5	2.4781229243139132E-5	0.23633445308694404	4.7497356049350006E-5
130-131	0.12835644196727608	2.436820875575348E-4	3.304163899085217E-5	2.4781229243139132E-5	0.23712332221785062	4.9562458486278264E-5
132-133	0.12838122319651918	2.436820875575348E-4	3.304163899085217E-5	2.4781229243139132E-5	0.23771394151481212	4.9562458486278264E-5
134-135	0.1283956789135777	2.436820875575348E-4	3.304163899085217E-5	2.4781229243139132E-5	0.2381744593582471	5.162756092320652E-5
136-137	0.1283956789135777	2.478122924313913E-4	3.304163899085217E-5	2.4781229243139132E-5	0.2387444276308393	5.369266336013478E-5
138-139	0.1283956789135777	2.498773948683196E-4	3.7171843864708695E-5	2.4781229243139132E-5	0.23927515895712986	5.369266336013478E-5
>>END_MODULE
