##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.control.4d.S.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22996710
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	66-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.06165855898518	37.0	35.0	38.0	33.0	39.0
2	35.37966874392033	35.0	34.0	37.0	33.0	39.0
3	35.50249048668266	35.0	34.0	37.0	33.0	39.0
4	35.565553463952014	36.0	35.0	38.0	33.0	39.0
5	35.48371832318623	36.0	35.0	38.0	33.0	39.0
6	35.43141149320925	35.0	35.0	37.0	33.0	39.0
7	35.476907653312146	35.0	34.0	38.0	33.0	39.0
8	35.54125094415679	36.0	35.0	38.0	33.0	39.0
9	35.56881127778713	36.0	35.0	38.0	33.0	39.0
10-14	35.541040105302024	35.8	35.0	37.8	33.0	39.0
15-19	35.51346728292873	36.0	35.0	38.0	33.0	39.0
20-24	35.50605656200387	36.0	35.0	38.0	33.0	39.0
25-29	35.48814237340906	36.0	35.0	38.0	33.0	39.0
30-34	35.47361789577727	36.0	34.8	38.0	33.0	39.0
35-39	35.44328885305767	36.0	34.2	38.0	33.0	39.0
40-44	35.43189631038527	36.0	34.0	38.0	33.0	39.0
45-49	35.40312763869266	36.0	34.0	38.0	32.8	39.0
50-54	35.38784230439919	36.0	34.0	38.0	32.4	39.0
55-59	35.35975853937368	36.0	34.0	38.0	32.0	39.0
60-64	35.32040727564943	36.0	34.0	38.0	32.0	39.0
65-69	35.29318099551824	36.0	34.0	38.0	32.0	39.0
70-74	35.26550443876433	36.0	34.0	38.0	32.0	39.0
75-79	35.213973796820945	36.0	34.0	38.0	32.0	39.0
80-84	35.17136569853466	36.0	34.0	38.0	32.0	39.0
85-89	35.12543246710152	36.0	34.0	38.0	32.0	39.0
90-94	35.074327108412255	36.0	34.0	38.0	32.0	39.0
95-99	35.00907664633107	35.6	34.0	38.0	31.0	39.0
100-104	34.96409874339572	35.4	34.0	38.0	31.0	39.0
105-109	34.90814037319533	35.0	34.0	38.0	31.0	39.0
110-114	34.830123446328734	35.0	34.0	37.2	31.0	39.0
115-119	34.76496850301278	35.0	34.0	37.0	31.0	39.0
120-124	34.69597788100631	35.0	34.0	37.0	30.2	39.0
125-129	34.61895218639022	35.0	34.0	37.0	30.0	39.0
130-134	34.518210951643205	35.0	34.0	37.0	30.0	39.0
135-139	34.46570495322085	35.0	34.0	37.0	30.0	39.0
140-144	34.35171165199657	35.0	34.0	37.0	29.4	39.0
145-149	34.34069109901473	35.0	34.0	37.0	29.2	39.0
150	35.12969151400668	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	162.0
22	1679.0
23	8934.0
24	30513.0
25	73332.0
26	141909.0
27	238727.0
28	350463.0
29	460454.0
30	590304.0
31	768350.0
32	1025100.0
33	1428786.0
34	2133887.0
35	3611873.0
36	9801708.0
37	2330516.0
38	6.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.002942159987235	18.740541581817574	15.183871953857746	32.072644304337445
2	24.18599443137736	25.88994686631262	32.35525864351901	17.568800058791016
3	20.099601203824374	29.64723214755502	29.261163879528855	20.99200276909175
4	23.749437365146463	34.187261316390966	21.413959710495966	20.6493416079666
5	25.438908587848815	35.00110146228794	21.584395014554698	17.97559493530855
6	19.258570045950513	38.88671490518834	21.95605742859507	19.898657620266075
7	20.66043638795976	20.840457521026636	36.556609445156326	21.942496645857275
8	21.752293086325086	23.548125568746748	27.89223516236788	26.807346182560288
9	23.670725073282224	23.63225000445716	27.529951023429007	25.167073898831617
10-14	23.211271929093716	28.882161472352546	25.073967973226917	22.832598625326817
15-19	23.21050880756421	27.761828539821565	26.647198664504618	22.380463988109604
20-24	23.108842960580013	27.692142049884527	26.582954692214667	22.616060297320793
25-29	23.179052133979162	27.272548116665384	26.586957003849683	22.96144274550577
30-34	23.06338689316863	26.99121569998491	26.813825977715943	23.131571429130517
35-39	23.3581490569738	26.70990763461382	26.902056859437717	23.029886448974658
40-44	23.535842505609214	26.352297579543315	26.92667713709202	23.18518277775545
45-49	23.42386715317104	26.17952654966732	26.992375865939085	23.404230431222555
50-54	23.640358120792058	26.21897219210922	26.682489799627863	23.45817988747086
55-59	23.649568133876542	26.23252108671197	26.72262075749096	23.395290021920527
60-64	23.349322577012103	26.18251741227332	26.809003548768494	23.65915646194608
65-69	23.57537757774837	26.104217858596574	26.827644900156443	23.49275966349861
70-74	23.7227304019115	26.077402220598668	26.54709571061315	23.65277166687668
75-79	23.505632075893498	26.19848316079792	26.824737651150777	23.471147112157805
80-84	23.839674766049985	26.186862125969885	26.51725004083636	23.45621306714377
85-89	23.52663411822619	26.279471270390893	26.780548463642678	23.41334614774024
90-94	23.56873612110936	25.981568536664884	26.82617963933559	23.623515702890167
95-99	23.576130651511633	26.127055734270826	26.84768969804176	23.449123916175783
100-104	23.815829517978617	25.959826468019862	26.76962214014293	23.454721873858592
105-109	23.633671056527223	26.165655716602167	26.643434618633812	23.5572386082368
110-114	23.73044177988541	26.27038983885769	26.495410560604633	23.503757820652265
115-119	23.892427655905994	26.377629866393654	26.645804835011887	23.08413764268846
120-124	23.863771877807714	26.558395002122076	26.73635698374554	22.841476136324665
125-129	23.815467910137027	26.624127641915273	26.561860709108764	22.99854373883894
130-134	23.972852629327505	26.67215079410401	26.57127447952815	22.78372209704034
135-139	23.737283130435156	26.694484563529958	26.84708260525773	22.72114970077716
140-144	23.776186816955054	26.8266870163329	26.60437192635975	22.792754240352302
145-149	23.646270006044436	27.313200536390212	26.218806997954875	22.821722459610477
150	23.780245224686915	26.058970642264693	26.47525484678478	23.685529286263613
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.5
10	2.5
11	3.5
12	5.5
13	7.5
14	13.5
15	27.0
16	44.5
17	75.5
18	157.5
19	287.5
20	450.5
21	627.5
22	943.0
23	1604.5
24	2627.5
25	4336.5
26	6825.0
27	10610.0
28	16117.5
29	22654.5
30	32032.5
31	46447.0
32	66763.0
33	91356.5
34	123209.0
35	174373.0
36	246015.5
37	331754.0
38	438745.0
39	572772.5
40	734765.5
41	911583.5
42	1096104.5
43	1278055.0
44	1421782.0
45	1519605.5
46	1552097.0
47	1522351.0
48	1483203.0
49	1444628.0
50	1349298.0
51	1198113.0
52	1020063.5
53	856558.0
54	727926.5
55	622508.0
56	514223.0
57	404055.5
58	331502.5
59	271525.5
60	203005.5
61	136573.0
62	85214.0
63	52524.5
64	32365.0
65	15896.5
66	8651.0
67	4800.0
68	2710.5
69	1654.5
70	823.0
71	567.0
72	370.0
73	255.5
74	211.0
75	141.5
76	91.0
77	35.5
78	12.0
79	2.0
80	0.5
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	4.348448104098369E-6
5	3.478758483278695E-5
6	5.65298253532788E-5
7	0.0022655414622352503
8	0.11414241428447808
9	0.0
10-14	1.7393792416393477E-6
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	8.696896208196738E-7
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	3.739780668821505E-5
110-114	0.019215746705183046
115-119	8.697406629904351E-7
120-124	0.0
125-129	2.6093505083915016E-6
130-134	0.047550710457646556
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
66-67	4.0
68-69	3.0
70-71	3.0
72-73	4.0
74-75	2.0
76-77	9.0
78-79	5.0
80-81	17.0
82-83	13.0
84-85	17.0
86-87	26.0
88-89	41.0
90-91	43.0
92-93	37.0
94-95	42.0
96-97	46.0
98-99	68.0
100-101	74.0
102-103	107.0
104-105	104.0
106-107	93.0
108-109	107.0
110-111	112.0
112-113	147.0
114-115	150.0
116-117	156.0
118-119	144.0
120-121	205.0
122-123	266.0
124-125	261.0
126-127	328.0
128-129	444.0
130-131	446.0
132-133	633.0
134-135	828.0
136-137	1515.0
138-139	4232.0
140-141	8193.0
142-143	9528.0
144-145	58344.0
146-147	750701.0
148-149	996987.0
150-151	2.1162225E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.19374093716474
#Duplication Level	Percentage of total
1	24.84422885589893
2	8.41125579826532
3	5.084851414968576
4	3.6349259136288117
5	2.7254008504796907
6	2.1341820807155636
7	1.79093848596889
8	1.512314035979154
9	1.2980133957255808
>10	16.75212211892624
>50	6.076815542178086
>100	13.56963542952917
>500	4.268512156427229
>1k	5.934430484577007
>5k	1.1568192792045495
>10k+	0.8055541575271443
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	32671	0.1420681480089978	No Hit
GAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCCG	23811	0.10354089780668625	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.043913672868858E-4	0.0	0.0	0.0	0.001965498543052463	0.0
2	0.0017959090669926263	4.348448104098369E-6	0.0	0.0	0.009340466527603297	0.0
3	0.002722128513165579	4.348448104098369E-6	0.0	0.0	0.013454098434080353	0.0
4	0.006622686462541816	4.348448104098369E-6	0.0	0.0	0.020094178689038563	0.0
5	0.0068357604196426355	4.348448104098369E-6	0.0	0.0	0.024455672137449227	0.0
6	0.008192476228121326	4.348448104098369E-6	0.0	0.0	0.0322480911399935	0.0
7	0.010684136991769693	4.348448104098369E-6	0.0	0.0	0.04313225674455172	0.0
8	0.018789644257809053	1.3045344312295107E-5	0.0	0.0	0.05873883699016077	0.0
9	0.01956801646844266	1.3045344312295107E-5	0.0	0.0	0.0703883294610403	0.0
10-11	0.020350737127180368	1.3045344312295107E-5	0.0	0.0	0.11914530382824326	0.0
12-13	0.021229123644208238	1.3045344312295107E-5	0.0	0.0	0.14242906920163798	0.0
14-15	0.025020970390982014	1.3045344312295107E-5	0.0	0.0	0.1536893755672007	0.0
16-17	0.029397683407757023	1.3045344312295107E-5	0.0	0.0	0.16163833870149252	0.0
18-19	0.03752710713836892	1.3045344312295107E-5	0.0	0.0	0.16980907268909334	0.0
20-21	0.044402003590948445	1.5219568364344292E-5	0.0	2.1742240520491845E-6	0.17447495750479092	0.0
22-23	0.04713500322437427	2.391646457254103E-5	0.0	4.348448104098369E-6	0.17954307377011755	0.0
24-25	0.053842484424946005	2.82649126766394E-5	0.0	4.348448104098369E-6	0.18424592039469995	0.0
26-27	0.059745502726259545	3.0439136728688583E-5	0.0	4.348448104098369E-6	0.18811821343139953	0.0
28-29	0.07361052950617719	3.0439136728688583E-5	0.0	4.348448104098369E-6	0.19233185964427085	0.0
30-31	0.10136232530653297	3.478758483278695E-5	0.0	8.696896208196738E-6	0.1966629139559528	0.0
32-33	0.12464391645587565	4.565870509303287E-5	0.0	8.696896208196738E-6	0.199985128307484	0.0
34-35	0.14139848700096666	5.870404940532798E-5	0.0	8.696896208196738E-6	0.20385307289607948	0.0
36-37	0.14456850566885437	7.174939371762309E-5	0.0	8.696896208196738E-6	0.20614905349504342	0.0
38-39	0.1473015053022802	7.827206587377063E-5	4.348448104098369E-6	8.696896208196738E-6	0.20776450196571597	0.0
40-41	0.14901914230339905	8.2620513977869E-5	8.696896208196738E-6	1.0871120260245923E-5	0.2094103895731172	0.0
42-43	0.14939963151250768	8.2620513977869E-5	1.3045344312295107E-5	1.3045344312295107E-5	0.21100409580326923	0.0
44-45	0.15040847147265848	8.696896208196738E-5	1.3045344312295107E-5	1.3045344312295107E-5	0.2124999619510791	0.0
46-47	0.15221090321180725	8.914318613401656E-5	1.3045344312295107E-5	1.3045344312295107E-5	0.21413497843822007	0.0
48-49	0.15399376693448757	9.78400823422133E-5	1.3045344312295107E-5	1.3045344312295107E-5	0.21533080166684715	0.0
50-51	0.15537222498348677	1.0001430639426248E-4	1.3045344312295107E-5	1.3045344312295107E-5	0.21672013083610656	0.0
52-53	0.1566115326931548	1.0001430639426248E-4	1.3045344312295107E-5	1.3045344312295107E-5	0.21797683233819098	0.0
54-55	0.1571246495694384	1.0001430639426248E-4	1.5219568364344292E-5	1.3045344312295107E-5	0.21913786798198526	2.1742240520491845E-6
56-57	0.1579073702281761	1.0001430639426248E-4	1.7393792416393476E-5	1.3045344312295107E-5	0.2203423881068205	4.348448104098369E-6
58-59	0.1581095730650167	1.0436275449836086E-4	1.7393792416393476E-5	1.3045344312295107E-5	0.22143819702905326	4.348448104098369E-6
60-61	0.15844875201713637	1.1305965070655758E-4	1.7393792416393476E-5	1.3045344312295107E-5	0.22233832578660165	4.348448104098369E-6
62-63	0.15878358252115193	1.1958232286270515E-4	1.7393792416393476E-5	1.3045344312295107E-5	0.22324062876820205	8.696896208196738E-6
64-65	0.15880532476167242	1.3045344312295109E-4	1.7393792416393476E-5	1.3045344312295107E-5	0.22406683390798077	8.696896208196738E-6
66-67	0.15890316484401465	1.3697611527909863E-4	1.7393792416393476E-5	1.3045344312295107E-5	0.2248256381021459	8.696896208196738E-6
68-69	0.15898578535799252	1.391503393311478E-4	1.7393792416393476E-5	1.3045344312295107E-5	0.22569097927486148	8.696896208196738E-6
70-71	0.1591010192327511	1.391503393311478E-4	1.7393792416393476E-5	1.3045344312295107E-5	0.22656284311973324	1.3045344312295107E-5
72-73	0.15913798104163596	1.4349878743524617E-4	1.956801646844266E-5	1.3045344312295107E-5	0.22716075473404673	1.3045344312295107E-5
74-75	0.1591575490581044	1.4784723553934453E-4	2.1742240520491845E-5	1.7393792416393476E-5	0.2278565064307025	1.3045344312295107E-5
76-77	0.1592010335391454	1.6089257985163962E-4	2.1742240520491845E-5	1.7393792416393476E-5	0.22867836312237708	1.3045344312295107E-5
78-79	0.1592814798290712	1.7393792416393476E-4	2.6090688624590214E-5	1.7393792416393476E-5	0.22986766367884798	1.956801646844266E-5
80-81	0.1593380096544245	1.7828637226803313E-4	2.6090688624590214E-5	1.7393792416393476E-5	0.2307264821794074	2.6090688624590214E-5
82-83	0.15935105499873678	1.826348203721315E-4	3.0439136728688583E-5	1.7393792416393476E-5	0.23158530067996683	2.6090688624590214E-5
84-85	0.1593836683595175	1.826348203721315E-4	3.0439136728688583E-5	1.7393792416393476E-5	0.23254413348692052	2.6090688624590214E-5
86-87	0.15942932706461055	1.826348203721315E-4	3.0439136728688583E-5	1.7393792416393476E-5	0.23338990664316767	3.0439136728688583E-5
88-89	0.15946846309754742	1.8698326847622985E-4	3.0439136728688583E-5	1.7393792416393476E-5	0.23418784687026972	3.0439136728688583E-5
90-91	0.15949890223427612	1.9133171658032824E-4	3.0439136728688583E-5	1.956801646844266E-5	0.23476401624406273	3.0439136728688583E-5
92-93	0.1595162960266925	1.9133171658032824E-4	3.0439136728688583E-5	2.1742240520491845E-5	0.23532496604949144	3.0439136728688583E-5
94-95	0.15954890938747326	1.9133171658032824E-4	3.0439136728688583E-5	2.1742240520491845E-5	0.23592722611190906	3.0439136728688583E-5
96-97	0.1595641289558376	1.956801646844266E-4	3.0439136728688583E-5	2.6090688624590214E-5	0.23655992531105535	3.0439136728688583E-5
98-99	0.15957934852420194	2.0002861278852496E-4	3.0439136728688583E-5	2.6090688624590214E-5	0.23709261020380742	3.0439136728688583E-5
100-101	0.15961196188498267	2.0002861278852496E-4	3.0439136728688583E-5	2.6090688624590214E-5	0.2376666053535484	3.0439136728688583E-5
102-103	0.1596184845571388	2.0002861278852496E-4	3.0439136728688583E-5	2.6090688624590214E-5	0.23820363869440456	3.478758483278695E-5
104-105	0.15963587834955523	2.0437706089262333E-4	3.0439136728688583E-5	2.6090688624590214E-5	0.23880807298087423	3.478758483278695E-5
106-107	0.15964457524576342	2.0437706089262333E-4	3.0439136728688583E-5	2.6090688624590214E-5	0.23933423520147012	3.478758483278695E-5
108-109	0.1596750143824921	2.0872550899672172E-4	3.0439136728688583E-5	2.6090688624590214E-5	0.23987779121448244	3.478758483278695E-5
110-111	0.15972502153568924	2.1524818115286926E-4	3.0439136728688583E-5	2.6090688624590214E-5	0.24041482455533858	3.478758483278695E-5
112-113	0.15973806688000153	2.1742240520491844E-4	3.261336078073777E-5	2.6090688624590214E-5	0.24100403927344388	3.478758483278695E-5
114-115	0.1597641575686261	2.1742240520491844E-4	3.478758483278695E-5	2.6090688624590214E-5	0.24156498907887258	3.478758483278695E-5
116-117	0.15978372558509457	2.1742240520491844E-4	3.478758483278695E-5	2.6090688624590214E-5	0.2421215904361972	3.478758483278695E-5
118-119	0.15978372558509457	2.1742240520491844E-4	3.478758483278695E-5	2.6090688624590214E-5	0.24264557843274104	3.478758483278695E-5
120-121	0.15979677092940686	2.1742240520491844E-4	3.478758483278695E-5	2.6090688624590214E-5	0.2432413158230025	3.913603293688532E-5
122-123	0.15980546782561506	2.1742240520491844E-4	3.478758483278695E-5	2.6090688624590214E-5	0.24382618209300375	3.913603293688532E-5
124-125	0.15980981627371915	2.261193014131152E-4	3.478758483278695E-5	2.6090688624590214E-5	0.2443806092262763	3.913603293688532E-5
126-127	0.15981416472182325	2.261193014131152E-4	3.478758483278695E-5	2.6090688624590214E-5	0.24493721058360088	3.913603293688532E-5
128-129	0.1598206873939794	2.3046774951721355E-4	3.478758483278695E-5	2.6090688624590214E-5	0.24560904581568407	4.783292914508206E-5
130-131	0.15983808118639578	2.3264197356926273E-4	3.6961808884836134E-5	2.6090688624590214E-5	0.24633741087312055	5.435560130122961E-5
132-133	0.15985764920286424	2.369904216733611E-4	3.913603293688532E-5	2.6090688624590214E-5	0.24702663989762014	5.65298253532788E-5
134-135	0.15987939144338473	2.3916464572541028E-4	3.913603293688532E-5	2.82649126766394E-5	0.24756802168658038	5.65298253532788E-5
136-137	0.15988591411554087	2.3916464572541028E-4	3.913603293688532E-5	3.0439136728688583E-5	0.24808548701096808	5.65298253532788E-5
138-139	0.15988808833959292	2.4351309382950867E-4	4.131025698893451E-5	3.0439136728688583E-5	0.24866600483286522	5.65298253532788E-5
>>END_MODULE
