##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.control.4d.S.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24113794
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	61-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.04531721553232	37.0	35.0	38.0	33.0	39.0
2	35.368250636959075	35.0	34.0	37.0	33.0	39.0
3	35.50400575703682	35.0	34.0	38.0	33.0	39.0
4	35.57533335484246	36.0	35.0	38.0	33.0	39.0
5	35.497055793045256	36.0	35.0	38.0	33.0	39.0
6	35.43831708938046	35.0	35.0	37.0	33.0	39.0
7	35.47771022676896	35.0	34.0	38.0	33.0	39.0
8	35.60766028771748	36.0	35.0	38.0	33.0	39.0
9	35.6070219808629	36.0	35.0	38.0	33.0	39.0
10-14	35.56101184243342	35.8	35.0	37.8	33.0	39.0
15-19	35.53063083312398	36.0	35.0	38.0	33.0	39.0
20-24	35.50359179480425	36.0	35.0	38.0	33.0	39.0
25-29	35.49829409673153	36.0	35.0	38.0	33.0	39.0
30-34	35.48692863512063	36.0	35.0	38.0	33.0	39.0
35-39	35.46137988074377	36.0	34.2	38.0	33.0	39.0
40-44	35.44598279308515	36.0	34.2	38.0	33.0	39.0
45-49	35.420521474140486	36.0	34.0	38.0	33.0	39.0
50-54	35.40224821527463	36.0	34.0	38.0	33.0	39.0
55-59	35.37458040820951	36.0	34.0	38.0	32.0	39.0
60-64	35.335099843584295	36.0	34.0	38.0	32.0	39.0
65-69	35.30276835498279	36.0	34.0	38.0	32.0	39.0
70-74	35.28577351965943	36.0	34.0	38.0	32.0	39.0
75-79	35.232208619435674	36.0	34.0	38.0	32.0	39.0
80-84	35.15281945280866	36.0	34.0	38.0	32.0	39.0
85-89	35.14745729769967	36.0	34.0	38.0	32.0	39.0
90-94	35.103732620238766	36.0	34.0	38.0	32.0	39.0
95-99	35.03972748000581	35.6	34.0	38.0	32.0	39.0
100-104	34.97706201592486	35.4	34.0	38.0	31.2	39.0
105-109	34.92800046119966	35.0	34.0	38.0	31.0	39.0
110-114	34.85916787492302	35.0	34.0	37.8	31.0	39.0
115-119	34.80114167293284	35.0	34.0	37.0	31.0	39.0
120-124	34.733635903209716	35.0	34.0	37.0	30.4	39.0
125-129	34.65925300531275	35.0	34.0	37.0	30.0	39.0
130-134	34.58346312407182	35.0	34.0	37.0	30.0	39.0
135-139	34.507852027662686	35.0	34.0	37.0	30.0	39.0
140-144	34.42094279705389	35.0	34.0	37.0	30.0	39.0
145-149	34.38503340925265	35.0	34.0	37.0	29.6	39.0
150	35.163410276742674	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	125.0
22	1408.0
23	7637.0
24	26094.0
25	65375.0
26	136150.0
27	243658.0
28	365327.0
29	481807.0
30	619494.0
31	799612.0
32	1065091.0
33	1480073.0
34	2213351.0
35	3779481.0
36	1.0467272E7
37	2361829.0
38	3.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.918768651668834	18.569612065193887	15.404668381922813	32.10695090121447
2	23.79617025808301	25.683380697652197	32.79968160959504	17.720767434669753
3	20.21471196112897	29.24479242047104	29.39518766727459	21.145307951125403
4	23.711386934797567	33.91915017603617	21.521984470797086	20.84747841836917
5	25.59277897123945	34.71903260017897	21.750857621160733	17.93733080742085
6	19.407700837122523	38.52444372710491	22.12112701966352	19.946728416109053
7	20.721135794723967	20.50060226938988	36.793102736135175	21.98515919975098
8	21.89494527489121	23.18305033210452	28.107787600740057	26.81421679226421
9	23.7668033491536	23.20485527909876	27.758340309285217	25.27000106246242
10-14	23.325839144184446	28.410289977595397	25.293578438963195	22.970292439256966
15-19	23.33546951040114	27.497042792163214	26.745828771248764	22.421658926186886
20-24	23.216981766881624	27.474125712129414	26.657025993724577	22.65186652726438
25-29	23.302279185100446	27.12985024256241	26.63767385588514	22.930196716452002
30-34	23.15466243097208	26.892523839259802	26.83146003486635	23.121353694901764
35-39	23.46897879280216	26.680049601485358	26.882700416201615	22.968271189510865
40-44	23.63978808146076	26.34611625196765	26.89668909007019	23.117406576501402
45-49	23.5247966371447	26.191159300772	26.970978519597537	23.313065542485766
50-54	23.72801369360658	26.23198306078213	26.674458445015954	23.36554480059533
55-59	23.758624627878962	26.23934168136296	26.722608644662056	23.279425046096023
60-64	23.4239876999195	26.212614063451333	26.840764091476377	23.52263414515279
65-69	23.658202545180764	26.138418825720827	26.848093183783845	23.355285445314568
70-74	23.791751287336034	26.11166538594718	26.570065341949118	23.52651798476767
75-79	23.635007389232815	26.22455857834314	26.830427480685444	23.310006551738603
80-84	23.964628680552284	26.224044103227566	26.51434133688801	23.296985879332137
85-89	23.650187854179315	26.319742107139444	26.771046484953892	23.25902355372735
90-94	23.686046002796694	26.040847040297905	26.798183878548087	23.474923078357314
95-99	23.68350155537599	26.20878379696468	26.842982202719217	23.264732444940115
100-104	23.935871667227268	25.99591718456628	26.768021100866555	23.300190047339896
105-109	23.747903465418965	26.236271957585522	26.64072638150987	23.375098195485638
110-114	23.83179342907645	26.313329814661245	26.53511912494384	23.319757631318467
115-119	23.97615343878234	26.42561396554254	26.69066340135852	22.9075691943166
120-124	23.953056262462106	26.599730973223483	26.78808787190563	22.659124892408784
125-129	23.906673994862853	26.650052696985778	26.633197624562882	22.810075683588487
130-134	24.062277246064962	26.648952212108462	26.68316897510712	22.605601566719457
135-139	23.83744684602948	26.65410517167897	26.96079206473848	22.547655917553065
140-144	23.857967443709676	26.812201822607	26.694727351658194	22.635103382025132
145-149	23.695842000386268	27.1951134468675	26.406183237638782	22.70286131510745
150	23.88529656227997	26.00578911761602	26.653097323918693	23.455816996185316
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	3.0
2	14.5
3	25.0
4	40.0
5	43.5
6	28.0
7	22.0
8	17.5
9	10.5
10	6.5
11	7.5
12	10.0
13	13.5
14	20.0
15	27.0
16	47.5
17	78.0
18	137.5
19	250.0
20	364.5
21	533.5
22	872.5
23	1440.0
24	2410.0
25	3899.0
26	6091.0
27	9493.5
28	14509.5
29	21039.0
30	30428.5
31	44289.5
32	64320.0
33	89895.5
34	123502.0
35	176745.5
36	252444.0
37	343078.5
38	457120.5
39	600176.5
40	772506.5
41	961367.5
42	1155192.5
43	1348095.5
44	1502300.0
45	1602625.5
46	1634966.0
47	1606268.0
48	1562286.0
49	1513765.0
50	1407681.0
51	1249381.5
52	1068434.0
53	892538.5
54	753707.0
55	649136.0
56	536374.5
57	425256.0
58	353806.5
59	290478.5
60	219632.5
61	147928.0
62	90598.0
63	54633.5
64	33899.5
65	16920.0
66	9104.0
67	4954.5
68	2639.0
69	1561.0
70	753.0
71	513.5
72	344.5
73	254.5
74	211.5
75	135.0
76	86.0
77	32.5
78	9.5
79	1.0
80	0.5
81	1.0
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	8.294007985636768E-6
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	8.294007985636769E-7
20-24	0.03722350783953782
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	2.4882023956910307E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	1.6588018860477917E-6
75-79	7.530963310979726E-4
80-84	0.07251380270363544
85-89	8.294039010299199E-7
90-94	0.0
95-99	8.294099959646715E-7
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	8.295942483567086E-7
140-144	3.7104141147539614E-4
145-149	0.037738102439592865
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
60-61	1.0
62-63	0.0
64-65	1.0
66-67	0.0
68-69	0.0
70-71	2.0
72-73	2.0
74-75	3.0
76-77	6.0
78-79	11.0
80-81	12.0
82-83	19.0
84-85	21.0
86-87	26.0
88-89	30.0
90-91	29.0
92-93	33.0
94-95	47.0
96-97	51.0
98-99	58.0
100-101	78.0
102-103	85.0
104-105	102.0
106-107	104.0
108-109	92.0
110-111	94.0
112-113	102.0
114-115	144.0
116-117	161.0
118-119	176.0
120-121	189.0
122-123	249.0
124-125	267.0
126-127	306.0
128-129	397.0
130-131	481.0
132-133	555.0
134-135	856.0
136-137	1468.0
138-139	4457.0
140-141	8458.0
142-143	9574.0
144-145	60936.0
146-147	791294.0
148-149	1040143.0
150-151	2.2192674E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.735891719371175
#Duplication Level	Percentage of total
1	25.262649423602145
2	8.472791027256033
3	5.252163297144507
4	3.635315754960545
5	2.817761734860944
6	2.1309871037785233
7	1.750734153524847
8	1.5588919624896653
9	1.3914031993581413
>10	16.877667099287176
>50	6.0556019670562655
>100	13.00032883825084
>500	4.213928281778386
>1k	5.840646553368043
>5k	1.0715237454100346
>10k+	0.6676058578739293
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	34518	0.143146283824105	No Hit
GAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCCG	27764	0.11513741885660962	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.3176031942547073E-4	1.2441011978455152E-5	0.0	0.0	0.0025628484675617614	0.0
2	0.0015343914773428022	1.2441011978455152E-5	0.0	0.0	0.009417846067690552	0.0
3	0.0022518231681003826	1.2441011978455152E-5	0.0	0.0	0.013734877224214488	0.0
4	0.005258401062893711	2.073501996409192E-5	0.0	0.0	0.020345201588766994	0.0
5	0.005420134218613628	2.073501996409192E-5	0.0	0.0	0.024857141932953394	0.0
6	0.006473473232789498	2.073501996409192E-5	0.0	0.0	0.03215586896031375	0.0
7	0.008480623165313596	4.147003992818384E-5	0.0	0.0	0.04391262527995387	0.0
8	0.015845702256559048	4.147003992818384E-5	0.0	0.0	0.05909480689766198	4.147003992818384E-6
9	0.01645116483951053	4.147003992818384E-5	0.0	0.0	0.07050736188589816	4.147003992818384E-6
10-11	0.017315815172013166	4.147003992818384E-5	0.0	0.0	0.11537587158619667	4.147003992818384E-6
12-13	0.018224009046440393	4.976404791382061E-5	0.0	0.0	0.13589524734266206	4.147003992818384E-6
14-15	0.021618331814562238	4.976404791382061E-5	0.0	0.0	0.1450850081907476	4.147003992818384E-6
16-17	0.025394178950023376	5.1837549910229805E-5	0.0	2.073501996409192E-6	0.1520789304246358	4.147003992818384E-6
18-19	0.03276755204925447	5.3911051906638993E-5	0.0	4.147003992818384E-6	0.1588509879449082	4.147003992818384E-6
20-21	0.03895902901053232	5.3911051906638993E-5	0.0	8.294007985636768E-6	0.16294408088581996	4.147003992818384E-6
22-23	0.0417997267456129	6.220505989227577E-5	0.0	8.294007985636768E-6	0.16733990511820745	4.147003992818384E-6
24-25	0.04815708386660349	7.257256987432172E-5	0.0	8.294007985636768E-6	0.1714392185651084	4.147003992818384E-6
26-27	0.05375761275890471	7.87930758635493E-5	0.0	8.294007985636768E-6	0.17496417195900404	4.147003992818384E-6
28-29	0.06828456774574752	7.87930758635493E-5	4.147003992818384E-6	8.294007985636768E-6	0.17810345398156757	4.147003992818384E-6
30-31	0.09502652299343686	8.708708384918607E-5	4.147003992818384E-6	8.294007985636768E-6	0.1815185117696535	4.147003992818384E-6
32-33	0.11493628916295792	9.745459383123204E-5	4.147003992818384E-6	1.036750998204596E-5	0.18389059805354563	4.147003992818384E-6
34-35	0.12893035413672357	1.0367509982045961E-4	4.147003992818384E-6	1.2441011978455152E-5	0.18643271150114327	4.147003992818384E-6
36-37	0.13118632430881677	1.0367509982045961E-4	4.147003992818384E-6	1.2441011978455152E-5	0.18813505664019525	4.147003992818384E-6
38-39	0.13332410486711466	1.0782210381327799E-4	4.147003992818384E-6	1.2441011978455152E-5	0.18940403986199766	4.147003992818384E-6
40-41	0.1348025117905544	1.0989560580968718E-4	4.147003992818384E-6	1.2441011978455152E-5	0.19074974265766723	4.147003992818384E-6
42-43	0.13506584654409837	1.1611611179891476E-4	4.147003992818384E-6	1.2441011978455152E-5	0.1922799871310172	4.147003992818384E-6
44-45	0.13586207131071948	1.3477762976659747E-4	4.147003992818384E-6	1.2441011978455152E-5	0.19358836689075143	4.147003992818384E-6
46-47	0.1373218167161916	1.4099813575582507E-4	4.147003992818384E-6	1.2441011978455152E-5	0.1949589517103779	4.147003992818384E-6
48-49	0.1385182273681197	1.5343914773428022E-4	4.147003992818384E-6	1.2441011978455152E-5	0.19607449578444602	6.220505989227576E-6
50-51	0.1395466843583386	1.555126497306894E-4	4.147003992818384E-6	1.2441011978455152E-5	0.19718381935252494	1.036750998204596E-5
52-53	0.14059172936452885	1.575861517270986E-4	4.147003992818384E-6	1.2441011978455152E-5	0.198187394318787	1.6588015971273536E-5
54-55	0.1410769288316886	1.575861517270986E-4	6.220505989227576E-6	1.2441011978455152E-5	0.1991598667551029	2.073501996409192E-5
56-57	0.14178399301246414	1.6380665771632618E-4	8.294007985636768E-6	1.2441011978455152E-5	0.2001468537053937	2.073501996409192E-5
58-59	0.14198097570212304	1.6795366170914455E-4	8.294007985636768E-6	1.2441011978455152E-5	0.20099491602192504	2.073501996409192E-5
60-61	0.14226919247962388	1.7624766969478133E-4	8.294007985636768E-6	1.2441011978455152E-5	0.20172686222665748	2.073501996409192E-5
62-63	0.14257607077509246	1.824681756840089E-4	8.294007985636768E-6	1.2441011978455152E-5	0.20254796901723554	2.073501996409192E-5
64-65	0.14262376132100987	1.9076218366964566E-4	8.294007985636768E-6	1.2441011978455152E-5	0.2033566347958351	2.073501996409192E-5
66-67	0.14271292190685547	2.0527669764451004E-4	8.294007985636768E-6	1.2441011978455152E-5	0.20402844944267168	2.073501996409192E-5
68-69	0.14277097996275492	2.135707056301468E-4	8.294007985636768E-6	1.2441011978455152E-5	0.20473966062744003	2.4882023956910305E-5
70-71	0.14290783109451793	2.2393821561219276E-4	8.294007985636768E-6	1.2441011978455152E-5	0.2054550188162012	3.317603194254707E-5
72-73	0.14295966864442816	2.363792275906479E-4	8.294007985636768E-6	1.2441011978455152E-5	0.20595265929533943	3.317603194254707E-5
74-75	0.14299491817836713	2.405262315834663E-4	8.294007985636768E-6	1.2441011978455152E-5	0.20653323985433397	3.317603194254707E-5
76-77	0.14301980020232402	2.5089374156551226E-4	8.294007985636768E-6	1.2441011978455152E-5	0.2072361570311167	3.317603194254707E-5
78-79	0.14306956425023787	2.654082555403766E-4	8.294007985636768E-6	1.2441011978455152E-5	0.20817130643149725	3.732303593536546E-5
80-81	0.1431276223061373	2.654082555403766E-4	1.2441011978455152E-5	1.4514513974864344E-5	0.2089260611581902	3.9396537931774646E-5
82-83	0.14316701884406907	2.6955525953319495E-4	1.2441011978455152E-5	1.6588015971273536E-5	0.20967874238288675	4.147003992818384E-5
84-85	0.14321470938998648	2.7370226352601336E-4	1.2441011978455152E-5	1.6588015971273536E-5	0.21042727660359045	4.147003992818384E-5
86-87	0.14325203242592185	2.7784926751883173E-4	1.6588015971273536E-5	1.6588015971273536E-5	0.21108457673645217	4.147003992818384E-5
88-89	0.1432852084578644	2.7992276951524096E-4	1.6588015971273536E-5	1.6588015971273536E-5	0.21172528885334263	4.147003992818384E-5
90-91	0.14329142896385363	2.861432755044685E-4	1.866151796768273E-5	1.6588015971273536E-5	0.21215450376659933	4.147003992818384E-5
92-93	0.14329557596784645	2.861432755044685E-4	2.073501996409192E-5	1.6588015971273536E-5	0.21262311521778782	4.147003992818384E-5
94-95	0.14334948701975309	2.923637814936961E-4	2.073501996409192E-5	1.6588015971273536E-5	0.21340897247442686	4.3543541924593037E-5
96-97	0.1433536340237459	3.006577894793329E-4	2.073501996409192E-5	1.6588015971273536E-5	0.21407871361926706	4.5617043921002225E-5
98-99	0.14336400153372797	3.027312914757421E-4	2.073501996409192E-5	1.6588015971273536E-5	0.2146323386523083	4.5617043921002225E-5
100-101	0.1433951040636741	3.027312914757421E-4	2.073501996409192E-5	1.6588015971273536E-5	0.21513619963743574	4.7690545917411414E-5
102-103	0.1434013245696633	3.027312914757421E-4	2.073501996409192E-5	1.6588015971273536E-5	0.21572300070241954	4.976404791382061E-5
104-105	0.14341376558164176	3.0687829546856044E-4	2.073501996409192E-5	1.6588015971273536E-5	0.21638652134127045	4.976404791382061E-5
106-107	0.1434407211075951	3.110252994613788E-4	2.073501996409192E-5	1.6588015971273536E-5	0.21690489684037279	4.976404791382061E-5
108-109	0.1434697501355448	3.193193074470156E-4	2.073501996409192E-5	1.6588015971273536E-5	0.21745644837141762	4.976404791382061E-5
110-111	0.1435029261674874	3.3798082541469833E-4	2.073501996409192E-5	1.6588015971273536E-5	0.2179478683445666	5.3911051906638993E-5
112-113	0.14351744068146224	3.4420133140392593E-4	2.073501996409192E-5	1.6588015971273536E-5	0.2184683173456653	5.3911051906638993E-5
114-115	0.14354439620741555	3.462748334003351E-4	2.073501996409192E-5	1.6588015971273536E-5	0.21894314930284303	5.8058055899457384E-5
116-117	0.14356305772538325	3.5249533938956266E-4	2.073501996409192E-5	1.6588015971273536E-5	0.2194387162799848	5.8058055899457384E-5
118-119	0.1435775722393581	3.5249533938956266E-4	2.4882023956910305E-5	2.073501996409192E-5	0.22002759084696502	5.8058055899457384E-5
120-121	0.14358171924335092	3.5249533938956266E-4	2.4882023956910305E-5	2.073501996409192E-5	0.2206828174778303	5.8058055899457384E-5
122-123	0.14359416025532937	3.56642343382381E-4	2.4882023956910305E-5	2.073501996409192E-5	0.22118045795696853	6.013155789586657E-5
124-125	0.14360867476930425	3.628628493716086E-4	2.9029027949728692E-5	2.073501996409192E-5	0.22169261295008158	6.220505989227577E-5
126-127	0.14362940978926833	3.732303593536546E-4	2.9029027949728692E-5	2.073501996409192E-5	0.22224623798312285	6.427856188868496E-5
128-129	0.14363977729925037	3.8774487332851896E-4	2.9029027949728692E-5	2.073501996409192E-5	0.22287658259003124	6.635206388509415E-5
130-131	0.1436439243032432	3.960388813141557E-4	2.9029027949728692E-5	2.073501996409192E-5	0.22350692719693965	7.049906787791254E-5
132-133	0.14367502683318933	4.022593873033833E-4	2.9029027949728692E-5	2.073501996409192E-5	0.22412483079186957	7.257256987432172E-5
134-135	0.1437123498691247	4.043328892997925E-4	3.317603194254707E-5	2.073501996409192E-5	0.22466394131093598	8.294007985636768E-5
136-137	0.14372479088110315	4.084798932926109E-4	3.317603194254707E-5	2.073501996409192E-5	0.22517816980604546	8.294007985636768E-5
138-139	0.14372686438309956	4.2299440726747517E-4	3.317603194254707E-5	2.073501996409192E-5	0.22570276581113696	8.294007985636768E-5
>>END_MODULE
