##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.2d.R.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23706065
Total Bases	3.5 Gbp
Sequences flagged as poor quality	0
Sequence length	53-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.98083819478264	37.0	35.0	38.0	33.0	39.0
2	35.33893959204111	35.0	34.0	37.0	33.0	39.0
3	35.45980992627836	35.0	34.0	37.0	33.0	39.0
4	35.517729576798175	36.0	35.0	38.0	33.0	39.0
5	35.43133927963161	36.0	35.0	37.0	33.0	39.0
6	35.38817796205317	35.0	35.0	37.0	33.0	39.0
7	35.41075100401522	35.0	34.0	37.0	33.0	39.0
8	35.50669413080577	36.0	35.0	38.0	33.0	39.0
9	35.51191148762985	36.0	35.0	38.0	33.0	39.0
10-14	35.49108060743106	35.8	35.0	37.8	33.0	39.0
15-19	35.46501600328861	36.0	35.0	38.0	33.0	39.0
20-24	35.451033362137494	36.0	34.8	38.0	33.0	39.0
25-29	35.4327338763308	36.0	34.4	38.0	33.0	39.0
30-34	35.414371368677166	36.0	34.2	38.0	33.0	39.0
35-39	35.38895252333106	36.0	34.0	38.0	33.0	39.0
40-44	35.37194213379571	36.0	34.0	38.0	33.0	39.0
45-49	35.344725976242785	36.0	34.0	38.0	32.6	39.0
50-54	35.32432632157993	36.0	34.0	38.0	32.0	39.0
55-59	35.3002109526845	36.0	34.0	38.0	32.0	39.0
60-64	35.260052434687275	36.0	34.0	38.0	32.0	39.0
65-69	35.236538644193004	36.0	34.0	38.0	32.0	39.0
70-74	35.209820949305794	36.0	34.0	38.0	32.0	39.0
75-79	35.16462256565043	36.0	34.0	38.0	32.0	39.0
80-84	35.12788666540905	36.0	34.0	38.0	32.0	39.0
85-89	35.081159745913524	35.6	34.0	37.8	32.0	39.0
90-94	35.03055669155873	35.0	34.0	37.0	32.0	39.0
95-99	34.970485446570166	35.0	34.0	37.0	31.0	39.0
100-104	34.926678512605896	35.0	34.0	37.0	31.0	39.0
105-109	34.86864916322101	35.0	34.0	37.0	31.0	39.0
110-114	34.801385934557615	35.0	34.0	37.0	31.0	39.0
115-119	34.74484289015526	35.0	34.0	37.0	31.0	39.0
120-124	34.6770027015427	35.0	34.0	37.0	30.4	39.0
125-129	34.60531092906852	35.0	34.0	37.0	30.0	39.0
130-134	34.51011235394397	35.0	34.0	37.0	30.0	39.0
135-139	34.459216031635734	35.0	34.0	37.0	30.0	39.0
140-144	34.34186709318183	35.0	34.0	37.0	29.4	39.0
145-149	34.338868895653654	35.0	34.0	37.0	29.2	39.0
150	35.114405094757345	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	15.0
21	200.0
22	1638.0
23	9108.0
24	31455.0
25	76604.0
26	148978.0
27	254405.0
28	372869.0
29	487009.0
30	619290.0
31	802268.0
32	1064777.0
33	1477905.0
34	2211898.0
35	3797245.0
36	1.0516543E7
37	1833850.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.59934261548679	18.969356576049208	16.67783328865419	30.753467519809803
2	24.209424887681696	25.52152792966694	32.92092129166102	17.348125890990342
3	20.23053257596875	29.44247935886784	29.927194999557923	20.399793065605493
4	23.756015496963794	33.92250328534097	22.31907508218467	20.002406135510565
5	25.213546680768268	35.131707684170856	22.50095735022668	17.1537882848342
6	19.05324630571982	38.91921801022938	22.830680401732234	19.19685528231857
7	20.384763273141214	20.76168447935151	37.972389090541704	20.88116315696557
8	21.675789404335415	23.434665333273085	28.190142037668085	26.69940322472342
9	23.569133046754068	23.431240064515137	28.54796863165608	24.45165825707472
10-14	23.12883390811592	29.253033770049985	25.633983539655357	21.98414878217874
15-19	22.951414332155085	28.32007927085326	27.138309964137868	21.590196432853787
20-24	23.050875799083485	28.355789963454498	27.056233077906434	21.537101159555583
25-29	23.137060494856485	28.274323047709522	27.000174849769458	21.588441607664535
30-34	23.124994774543893	28.03205977933596	27.235497367201134	21.607448078919013
35-39	23.21371260898846	28.008229117738438	27.20158406719968	21.576474206073428
40-44	23.34725564955635	27.76698621217819	27.327118186843748	21.558639951421714
45-49	23.34446142790885	27.614290267068785	27.402513238700728	21.638735066321637
50-54	23.406183383080943	27.576312876694743	27.368730553710456	21.648773186513857
55-59	23.588006204555985	27.46294389033071	27.26553870153678	21.68351120357653
60-64	23.494718629575903	27.439225146748324	27.324934553662494	21.74112167001328
65-69	23.598264396617687	27.373450803375132	27.265235441136888	21.763049358870294
70-74	23.675353870241437	27.328337246863438	27.17480258898655	21.821506293908573
75-79	23.595837236458827	27.410877864693322	27.233629924047335	21.759654974800515
80-84	23.713445115655833	27.436100598606377	27.076167341498813	21.774286944238973
85-89	23.738752891633887	27.382356070975188	27.134930507096193	21.743960530294732
90-94	23.681074693725044	27.321430308521737	27.189359401175285	21.808135596577934
95-99	23.740553399605087	27.41833105101553	27.201017858505434	21.64009769087395
100-104	23.925365298754787	27.394029859384883	27.0047964483335	21.67580839352683
105-109	23.712596909508157	27.535809677369738	27.110932416891064	21.64066099623104
110-114	23.75577766611151	27.542411915803633	27.020756929874324	21.681053488210534
115-119	23.813824164519563	27.523311573164992	27.06622653570515	21.596637726610293
120-124	23.785574866585748	27.449681167755973	27.220010301517465	21.54473366414081
125-129	23.763182582817098	27.540320164177167	27.179125364909734	21.517371888095997
130-134	23.82254023054718	27.440615878776807	27.250855642113216	21.485988248562794
135-139	23.724525014244023	27.459641792450544	27.338495643746736	21.477337549558694
140-144	23.764329235019108	27.584809519477005	27.29932653613955	21.35153470936434
145-149	23.54185464701282	27.87396737969573	27.043267755671778	21.540910217619672
150	23.657877712661023	26.552697232588017	27.483334807085257	22.306090247665697
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	1.5
9	0.5
10	1.5
11	3.5
12	9.5
13	16.5
14	16.5
15	30.0
16	63.0
17	120.0
18	193.0
19	368.0
20	604.5
21	876.5
22	1365.5
23	2349.0
24	4074.5
25	6775.0
26	10586.5
27	16683.0
28	27104.5
29	40589.5
30	55854.0
31	75331.5
32	101637.5
33	136029.5
34	182596.0
35	252361.5
36	351809.0
37	470865.0
38	611971.5
39	793744.0
40	1006273.5
41	1205940.0
42	1383568.0
43	1546148.5
44	1662100.0
45	1711977.5
46	1707715.5
47	1656574.5
48	1555005.5
49	1409356.5
50	1228815.0
51	1031579.0
52	840108.0
53	669609.5
54	522494.5
55	394336.0
56	291814.5
57	215069.0
58	157177.0
59	113756.5
60	79394.0
61	53442.0
62	36356.0
63	25121.5
64	17131.5
65	11492.0
66	8198.5
67	6254.5
68	5848.5
69	4304.0
70	1934.5
71	1183.0
72	685.0
73	481.5
74	402.5
75	262.0
76	177.0
77	68.0
78	13.0
79	1.5
80	0.5
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	4.2183297818511845E-6
4	8.436659563702369E-6
5	2.9528308472958293E-5
6	1.096765743281308E-4
7	0.0024466312734736867
8	0.1144643786305319
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	1.6873319127404737E-6
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	4.640505208258364E-5
110-114	0.019466918287610487
115-119	0.0
120-124	0.0
125-129	3.3752973362710346E-6
130-134	0.04748377670680798
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
52-53	1.0
54-55	0.0
56-57	0.0
58-59	1.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	3.0
68-69	2.0
70-71	7.0
72-73	15.0
74-75	13.0
76-77	34.0
78-79	39.0
80-81	50.0
82-83	75.0
84-85	61.0
86-87	98.0
88-89	92.0
90-91	130.0
92-93	114.0
94-95	118.0
96-97	136.0
98-99	154.0
100-101	171.0
102-103	169.0
104-105	172.0
106-107	187.0
108-109	201.0
110-111	210.0
112-113	228.0
114-115	213.0
116-117	238.0
118-119	271.0
120-121	343.0
122-123	317.0
124-125	375.0
126-127	401.0
128-129	475.0
130-131	600.0
132-133	797.0
134-135	1010.0
136-137	1536.0
138-139	3327.0
140-141	7122.0
142-143	10752.0
144-145	68548.0
146-147	820507.0
148-149	1028228.0
150-151	2.1758524E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.090943082600425
#Duplication Level	Percentage of total
1	29.93622068812676
2	10.579269658961557
3	6.847620653628918
4	5.058573293656854
5	3.867688047740185
6	3.0302978386886457
7	2.3860783477419942
8	2.015606535179506
9	1.7801870547146215
>10	20.588843391788718
>50	5.24020337613767
>100	6.379754500949093
>500	1.1670356735058502
>1k	0.9816618224715171
>5k	0.09673330321331018
>10k+	0.04422581349479231
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.2183297818511845E-6	8.436659563702369E-6	0.0	0.0	8.647576052794928E-4	0.0
2	2.5309978691107107E-5	2.109164890925592E-5	0.0	0.0	0.005260257237968427	0.0
3	6.327494672776777E-5	2.5309978691107107E-5	0.0	0.0	0.006913842512454091	0.0
4	1.1811323389183316E-4	2.5309978691107107E-5	4.2183297818511845E-6	0.0	0.00818777810657315	4.2183297818511845E-6
5	1.2233156367368435E-4	2.5309978691107107E-5	4.2183297818511845E-6	0.0	0.009816053402367707	4.2183297818511845E-6
6	1.645148614921962E-4	2.5309978691107107E-5	4.2183297818511845E-6	4.2183297818511845E-6	0.011402145400343752	4.2183297818511845E-6
7	1.645148614921962E-4	2.952830847295829E-5	4.2183297818511845E-6	4.2183297818511845E-6	0.01349865530192379	4.2183297818511845E-6
8	1.898248401833033E-4	3.3746638254809476E-5	4.2183297818511845E-6	4.2183297818511845E-6	0.018995139007675885	4.2183297818511845E-6
9	2.0247982952885686E-4	3.3746638254809476E-5	4.2183297818511845E-6	4.2183297818511845E-6	0.024765814149248305	4.2183297818511845E-6
10-11	2.214623135471872E-4	3.3746638254809476E-5	4.2183297818511845E-6	4.2183297818511845E-6	0.031150256274080072	4.2183297818511845E-6
12-13	2.530997869110711E-4	3.585580314573507E-5	4.2183297818511845E-6	4.2183297818511845E-6	0.03720566867592745	4.2183297818511845E-6
14-15	2.973922496205085E-4	3.796496803666066E-5	4.2183297818511845E-6	4.2183297818511845E-6	0.0449737229692064	4.2183297818511845E-6
16-17	3.227022283116156E-4	3.796496803666066E-5	4.2183297818511845E-6	4.2183297818511845E-6	0.050577774084395705	4.2183297818511845E-6
18-19	3.859771750393834E-4	3.796496803666066E-5	4.2183297818511845E-6	4.2183297818511845E-6	0.058617910648604055	6.327494672776777E-6
20-21	4.429246270943744E-4	4.007413292758625E-5	4.2183297818511845E-6	4.2183297818511845E-6	0.06434218416257612	8.436659563702369E-6
22-23	4.893262546947374E-4	7.171160629147013E-5	8.436659563702369E-6	4.2183297818511845E-6	0.07005591185209355	8.436659563702369E-6
24-25	5.315095525132492E-4	7.171160629147013E-5	8.436659563702369E-6	4.2183297818511845E-6	0.07547857478666324	8.436659563702369E-6
26-27	5.758020152226867E-4	7.382077118239573E-5	8.436659563702369E-6	8.436659563702369E-6	0.07961464713776834	8.436659563702369E-6
28-29	6.770419299871151E-4	8.01482658551725E-5	8.436659563702369E-6	1.2654989345553554E-5	0.08354613049445364	8.436659563702369E-6
30-31	8.162468127882042E-4	9.069409030980046E-5	1.2654989345553554E-5	1.2654989345553554E-5	0.08855328794551098	8.436659563702369E-6
32-33	9.195958924435582E-4	9.280325520072606E-5	1.2654989345553554E-5	1.2654989345553554E-5	0.09365535781666	1.2654989345553554E-5
34-35	0.0010145083125352099	1.0123991476442843E-4	1.2654989345553554E-5	1.2654989345553554E-5	0.09681277765837562	1.2654989345553554E-5
36-37	0.0010883290837176056	1.0545824454627961E-4	1.2654989345553554E-5	1.4764154236479145E-5	0.10027602640927544	1.2654989345553554E-5
38-39	0.0011368398762088942	1.0545824454627961E-4	1.4764154236479145E-5	1.8982484018330328E-5	0.10345242873500937	1.4764154236479145E-5
40-41	0.0011916781633729595	1.075674094372052E-4	1.6873319127404738E-5	2.109164890925592E-5	0.10622176223679466	1.6873319127404738E-5
42-43	0.0012169881420640667	1.1811323389183316E-4	1.6873319127404738E-5	2.109164890925592E-5	0.10901640571727109	1.6873319127404738E-5
44-45	0.001252843945209802	1.349865530192379E-4	1.6873319127404738E-5	2.109164890925592E-5	0.11129008546968888	1.6873319127404738E-5
46-47	0.0013372105408468254	1.624056966012706E-4	1.6873319127404738E-5	2.109164890925592E-5	0.11387592162596366	1.6873319127404738E-5
48-49	0.0013941579929018164	1.666240263831218E-4	2.109164890925592E-5	2.109164890925592E-5	0.1161791296868544	1.6873319127404738E-5
50-51	0.0014447779502840307	1.6873319127404737E-4	2.109164890925592E-5	2.109164890925592E-5	0.11820814631192482	1.6873319127404738E-5
52-53	0.0014975070725571705	1.7295152105589856E-4	2.109164890925592E-5	2.109164890925592E-5	0.12041433278783299	1.8982484018330328E-5
54-55	0.0015628911841758639	1.7716985083774975E-4	2.3200813800181514E-5	2.5309978691107107E-5	0.12235265532259361	2.3200813800181514E-5
56-57	0.0016198386362308549	1.8138818061960095E-4	2.5309978691107107E-5	2.5309978691107107E-5	0.12433948864984551	2.5309978691107107E-5
58-59	0.0016683494287221435	1.961523348560801E-4	2.5309978691107107E-5	2.5309978691107107E-5	0.12613227880713226	2.5309978691107107E-5
60-61	0.0017421701999045392	2.130256539834848E-4	2.5309978691107107E-5	2.5309978691107107E-5	0.12747792600754282	3.163747336388388E-5
62-63	0.0017864626626139767	2.193531486562616E-4	2.5309978691107107E-5	2.5309978691107107E-5	0.1293909385636123	3.3746638254809476E-5
64-65	0.0017970084870686045	2.3411730289274072E-4	2.5309978691107107E-5	2.5309978691107107E-5	0.13107616131146185	3.3746638254809476E-5
66-67	0.0018687200933600748	2.63645611365699E-4	2.5309978691107107E-5	2.952830847295829E-5	0.13233744191623537	3.3746638254809476E-5
68-69	0.0018855934124874794	2.699731060384758E-4	2.5309978691107107E-5	2.952830847295829E-5	0.13369785327088235	3.7964968036660656E-5
70-71	0.0019151217209604377	2.699731060384758E-4	2.5309978691107107E-5	2.952830847295829E-5	0.1350202996574927	4.640162760036303E-5
72-73	0.0019383225347606192	2.699731060384758E-4	2.5309978691107107E-5	2.952830847295829E-5	0.13602848047535515	4.640162760036303E-5
74-75	0.0019636325134517263	2.889555900568062E-4	2.952830847295829E-5	3.796496803666066E-5	0.13714844703243662	4.640162760036303E-5
76-77	0.0019847241623609825	3.1215640385698763E-4	2.952830847295829E-5	3.796496803666066E-5	0.13850674922219272	4.640162760036303E-5
78-79	0.0020437807793068988	3.353572176571692E-4	2.952830847295829E-5	3.796496803666066E-5	0.14014135201266006	4.8510792491288625E-5
80-81	0.002064872428216155	3.4168471232994593E-4	2.952830847295829E-5	3.796496803666066E-5	0.1415966757873987	5.48382871640654E-5
82-83	0.0020986190664709645	3.4168471232994593E-4	3.163747336388388E-5	3.796496803666066E-5	0.1430730912110466	5.48382871640654E-5
84-85	0.002126038210052997	3.459030421117971E-4	3.3746638254809476E-5	3.796496803666066E-5	0.14454950663469454	5.48382871640654E-5
86-87	0.002151348188744104	3.6488552613012743E-4	3.796496803666066E-5	3.796496803666066E-5	0.14637393426534517	5.48382871640654E-5
88-89	0.002168221507871509	3.859771750393834E-4	3.796496803666066E-5	4.007413292758625E-5	0.14804650202384917	5.48382871640654E-5
90-91	0.002193531486562616	4.1972381329419284E-4	3.796496803666066E-5	4.218329781851184E-5	0.148987189565202	5.48382871640654E-5
92-93	0.0022019681461263184	4.2183297818511843E-4	4.007413292758625E-5	4.218329781851184E-5	0.15003122618621018	5.48382871640654E-5
94-95	0.002223059795035574	4.323788026397464E-4	4.218329781851184E-5	5.0619957382214214E-5	0.15100355120092684	5.48382871640654E-5
96-97	0.0022272781248174256	4.534704515490023E-4	4.218329781851184E-5	5.48382871640654E-5	0.15186830880620633	5.905661694591659E-5
98-99	0.002250478938617607	4.576887813308535E-4	4.218329781851184E-5	5.48382871640654E-5	0.1527372847412677	6.749327650961895E-5
100-101	0.0022589155981813094	4.7034377067640704E-4	4.218329781851184E-5	5.48382871640654E-5	0.15351345742112832	6.749327650961895E-5
102-103	0.002261024763072235	4.851079249128862E-4	4.218329781851184E-5	5.48382871640654E-5	0.15424955596806134	6.749327650961895E-5
104-105	0.002265243092854086	4.935445844765886E-4	4.218329781851184E-5	5.48382871640654E-5	0.15503416530748565	6.749327650961895E-5
106-107	0.0023095355555635237	4.977629142584398E-4	4.218329781851184E-5	5.48382871640654E-5	0.15580822882245535	6.749327650961895E-5
108-109	0.0023158630502363004	5.104179036039933E-4	4.8510792491288625E-5	5.48382871640654E-5	0.1565696373480795	7.171160629147013E-5
110-111	0.0023158630502363004	5.230728929495468E-4	5.2729122273139804E-5	5.48382871640654E-5	0.1573943208204314	7.171160629147013E-5
112-113	0.0023327363693637047	5.27291222731398E-4	5.694745205499099E-5	5.48382871640654E-5	0.15815572934605554	7.382077118239573E-5
114-115	0.0023411730289274076	5.420553769678772E-4	5.905661694591658E-5	5.48382871640654E-5	0.1590015044673167	7.592993607332132E-5
116-117	0.0023559371831638866	5.48382871640654E-4	5.905661694591658E-5	5.48382871640654E-5	0.1598746987321599	8.22574307460981E-5
118-119	0.0023622646778366633	5.526012014225052E-4	6.327494672776777E-5	5.48382871640654E-5	0.16070570969918457	8.436659563702368E-5
120-121	0.0023622646778366633	5.610378609862076E-4	6.327494672776777E-5	5.48382871640654E-5	0.1615472664906639	8.436659563702368E-5
122-123	0.0023622646778366633	5.631470258771332E-4	6.327494672776777E-5	5.48382871640654E-5	0.16231922084074266	9.280325520072606E-5
124-125	0.00236859217250944	5.715836854408355E-4	6.327494672776777E-5	5.48382871640654E-5	0.1631544501375492	1.0123991476442843E-4
126-127	0.0023812471618549933	5.779111801136122E-4	6.327494672776777E-5	5.905661694591658E-5	0.1639960069290285	1.0123991476442843E-4
128-129	0.002391792986309622	5.884570045682402E-4	6.327494672776777E-5	5.905661694591658E-5	0.16494513112994502	1.096765743281308E-4
130-131	0.002410775470327952	5.968936641319426E-4	6.327494672776777E-5	5.905661694591658E-5	0.1659069103202071	1.1811323389183316E-4
132-133	0.0024403037788009105	6.032211588047194E-4	6.327494672776777E-5	5.905661694591658E-5	0.16672948462766807	1.2233156367368435E-4
134-135	0.0024635045926010915	6.137669832593474E-4	6.327494672776777E-5	5.905661694591658E-5	0.1675309672862198	1.2444072856460995E-4
136-137	0.0024719412521647943	6.285311374958265E-4	6.327494672776777E-5	6.116578183684218E-5	0.16833455910966244	1.328773881283123E-4
138-139	0.0024719412521647943	6.369677970595288E-4	6.327494672776777E-5	6.749327650961895E-5	0.1690727668214864	1.4553237747386586E-4
>>END_MODULE
