##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.2d.R.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24165536
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	63-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.98687941372374	37.0	35.0	38.0	33.0	39.0
2	35.31743732065368	35.0	34.0	37.0	33.0	39.0
3	35.45845157334809	35.0	34.0	37.0	33.0	39.0
4	35.5140831968304	36.0	35.0	38.0	33.0	39.0
5	35.428916039768374	36.0	35.0	37.0	33.0	39.0
6	35.38673994237082	35.0	34.0	37.0	33.0	39.0
7	35.41227092997234	35.0	34.0	37.0	33.0	39.0
8	35.541084129067116	36.0	35.0	38.0	33.0	39.0
9	35.50142227343933	36.0	34.0	38.0	33.0	39.0
10-14	35.4813007996181	35.8	34.4	37.8	33.0	39.0
15-19	35.458160497660806	36.0	34.2	38.0	33.0	39.0
20-24	35.439662219782754	36.0	34.2	38.0	33.0	39.0
25-29	35.42223947360406	36.0	34.0	38.0	33.0	39.0
30-34	35.400006505131934	36.0	34.0	38.0	33.0	39.0
35-39	35.373936849569574	36.0	34.0	38.0	33.0	39.0
40-44	35.35365751456951	36.0	34.0	38.0	32.8	39.0
45-49	35.32451322412216	36.0	34.0	38.0	32.0	39.0
50-54	35.29932301108487	36.0	34.0	38.0	32.0	39.0
55-59	35.273664064393195	36.0	34.0	38.0	32.0	39.0
60-64	35.230715587907696	36.0	34.0	38.0	32.0	39.0
65-69	35.202487337560946	35.8	34.0	38.0	32.0	39.0
70-74	35.16859095736636	35.8	34.0	38.0	32.0	39.0
75-79	35.12101941933336	35.4	34.0	37.4	32.0	39.0
80-84	35.078670563191295	35.2	34.0	37.2	32.0	39.0
85-89	35.0262076891147	35.0	34.0	37.0	32.0	39.0
90-94	34.96844687484882	35.0	34.0	37.0	31.4	39.0
95-99	34.903172657138	35.0	34.0	37.0	31.0	39.0
100-104	34.85219704137869	35.0	34.0	37.0	31.0	39.0
105-109	34.78753128892874	35.0	34.0	37.0	31.0	39.0
110-114	34.71943503376012	35.0	34.0	37.0	31.0	39.0
115-119	34.649890911954714	35.0	34.0	37.0	30.0	39.0
120-124	34.57200236989238	35.0	34.0	37.0	30.0	39.0
125-129	34.49372844888285	35.0	34.0	37.0	30.0	39.0
130-134	34.39041271620502	35.0	34.0	37.0	29.8	39.0
135-139	34.33013688894638	35.0	34.0	37.0	29.8	39.0
140-144	34.20382646631171	35.0	34.0	37.0	29.0	39.0
145-149	34.19767361272581	35.0	34.0	37.0	29.0	39.0
150	34.99926559018235	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	10.0
21	172.0
22	1683.0
23	9529.0
24	32911.0
25	80150.0
26	155917.0
27	261824.0
28	382817.0
29	504839.0
30	647056.0
31	844861.0
32	1129480.0
33	1570214.0
34	2356607.0
35	4003776.0
36	1.049967E7
37	1684010.0
38	10.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.61434648087259	18.815229258726145	16.142182817711966	31.42824144268929
2	23.959878233199543	25.852817831145973	33.30891977732255	16.878384158331933
3	19.696873266125774	29.76053996898724	29.924376599798986	20.618210165087998
4	23.320575219188186	34.31472407646989	22.35619768582828	20.008503018513636
5	24.906668736832486	35.321670498018335	22.57265057145846	17.199010193690718
6	18.786415496846416	38.86899922269467	23.056629077045923	19.287956203412993
7	20.103816443384495	20.712745622526228	38.11296798879197	21.0704699452973
8	21.41441017488708	23.354209896275425	28.400172874295027	26.831207054542467
9	23.35546374804184	23.373857712073924	28.727237831596202	24.543440708288035
10-14	23.015249485879394	29.21093080658339	25.67475681069106	22.099062896846153
15-19	22.828471092054404	28.326995105757224	27.17948900450625	21.66504479768212
20-24	22.925322244042093	28.31709836686428	27.119730346556352	21.637849042537272
25-29	23.003258028292855	28.256210828512142	27.050578145669935	21.68995299752507
30-34	23.01355285477632	27.995635602702958	27.27193553662538	21.71887600589534
35-39	23.104460195747038	27.974212762954753	27.239732048482036	21.681594992816176
40-44	23.234519606765602	27.733741970382948	27.369279125445427	21.662459297406027
45-49	23.25024199752904	27.565523065575704	27.437590459404664	21.746644477490587
50-54	23.305212845268567	27.52896107911697	27.395965063634424	21.769861011980034
55-59	23.490547861218555	27.41910876713018	27.28996782856379	21.800375543087476
60-64	23.40197977319419	27.387913327375923	27.34964477799826	21.860462121431627
65-69	23.50675732759443	27.32060380749942	27.27933924526943	21.893299619636725
70-74	23.56934962290558	27.286273022933738	27.187118495577717	21.957258858582964
75-79	23.52301356983313	27.37684614561953	27.221370492183066	21.87876979236427
80-84	23.632182243649392	27.412017520208977	27.080797520473983	21.875002715667648
85-89	23.679865726497457	27.35222079461852	27.131614447418627	21.836299031465394
90-94	23.61383933823691	27.290762779708693	27.187735050474295	21.907662831580105
95-99	23.691117734891552	27.384492676352913	27.18887417650902	21.735515412246517
100-104	23.882798781643984	27.364879894540888	26.982576632954757	21.769744690860364
105-109	23.66571056705316	27.519149658099597	27.077951481112635	21.737188293734615
110-114	23.684994921401767	27.523633486753656	27.00079442051383	21.790577171330746
115-119	23.771579917673773	27.508402285210337	27.032500281059807	21.687517516056083
120-124	23.750284494553288	27.42649010476311	27.20584939426407	21.61737600641953
125-129	23.719763360491875	27.52050085896788	27.1498460043209	21.609889776219347
130-134	23.784773604782675	27.418407839359748	27.235323257761852	21.561495298095725
135-139	23.688011877628014	27.43259909189305	27.32623137172861	21.553157658750326
140-144	23.751310687868994	27.54951473928057	27.292666267535314	21.40650830531512
145-149	23.493086850689533	27.727210060157248	27.167091658597908	21.612611430555308
150	23.621907154130355	26.46408849755678	27.622625853681438	22.291378494631424
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	0.0
7	2.0
8	4.0
9	3.0
10	4.0
11	7.5
12	9.5
13	12.0
14	21.5
15	39.5
16	68.5
17	138.5
18	197.5
19	317.5
20	548.0
21	875.5
22	1352.0
23	2260.0
24	3890.0
25	6417.5
26	9950.0
27	15901.0
28	26478.0
29	40452.5
30	55638.5
31	74628.5
32	100853.0
33	135563.0
34	182685.5
35	254321.0
36	356566.5
37	479098.0
38	625065.0
39	812291.5
40	1034301.5
41	1241516.5
42	1421637.0
43	1583817.5
44	1696270.5
45	1746158.0
46	1740149.0
47	1685375.0
48	1583986.0
49	1435699.0
50	1249606.5
51	1047918.5
52	855213.0
53	681743.5
54	530863.0
55	402281.5
56	298192.0
57	219542.5
58	159811.0
59	114937.0
60	80061.5
61	53542.0
62	36425.5
63	25581.5
64	17457.5
65	11461.0
66	8065.0
67	5869.5
68	4693.0
69	3351.5
70	1721.5
71	1046.5
72	579.5
73	420.0
74	344.5
75	214.0
76	137.0
77	54.0
78	12.0
79	0.5
80	0.0
81	0.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	8.276249283276813E-7
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	8.276807565796689E-7
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	2.483427364861299E-6
130-134	0.05082104785470406
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62-63	1.0
64-65	2.0
66-67	4.0
68-69	7.0
70-71	9.0
72-73	12.0
74-75	30.0
76-77	27.0
78-79	53.0
80-81	62.0
82-83	68.0
84-85	76.0
86-87	111.0
88-89	154.0
90-91	171.0
92-93	141.0
94-95	146.0
96-97	155.0
98-99	193.0
100-101	204.0
102-103	203.0
104-105	219.0
106-107	213.0
108-109	258.0
110-111	257.0
112-113	235.0
114-115	274.0
116-117	265.0
118-119	348.0
120-121	358.0
122-123	405.0
124-125	431.0
126-127	547.0
128-129	599.0
130-131	767.0
132-133	932.0
134-135	1268.0
136-137	1866.0
138-139	3800.0
140-141	7729.0
142-143	11536.0
144-145	70913.0
146-147	838016.0
148-149	1071351.0
150-151	2.215112E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.33851837782765
#Duplication Level	Percentage of total
1	28.167238004210464
2	10.485277124142932
3	6.916765959719296
4	5.018401393320593
5	3.762766882484694
6	3.019188975777271
7	2.532642686011106
8	2.120742835925527
9	1.8591779291979083
>10	21.190135049093026
>50	5.475197213838818
>100	6.6803976755357315
>500	1.3595100175147576
>1k	1.2354323489198136
>5k	0.07674342015611699
>10k+	0.10038248415195565
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.138124641638406E-6	1.6552498566553625E-5	0.0	0.0	6.414093194539529E-4	0.0
2	8.276249283276813E-6	3.310499713310725E-5	0.0	0.0	0.004804362708942189	0.0
3	2.8966872491468844E-5	3.310499713310725E-5	0.0	0.0	0.006430645693106083	0.0
4	9.931499139932175E-5	3.724312177474565E-5	0.0	0.0	0.007792088700205119	0.0
5	9.931499139932175E-5	3.724312177474565E-5	0.0	0.0	0.009352161690102797	0.0
6	1.0345311604096015E-4	4.138124641638406E-5	0.0	4.138124641638406E-6	0.011023964045324713	0.0
7	1.1586748996587537E-4	4.965749569966087E-5	0.0	4.138124641638406E-6	0.01305578324436917	0.0
8	1.6552498566553623E-4	5.379562034129928E-5	0.0	4.138124641638406E-6	0.01830292528996667	0.0
9	1.6966311030717465E-4	6.20718696245761E-5	0.0	4.138124641638406E-6	0.024108714162185355	0.0
10-11	1.8621560887372828E-4	6.62099942662145E-5	0.0	4.138124641638406E-6	0.030137961765052512	0.0
12-13	2.1932060600683554E-4	8.276249283276812E-5	0.0	4.138124641638406E-6	0.03587754064300498	0.0
14-15	2.793234133105924E-4	8.483155515358733E-5	0.0	4.138124641638406E-6	0.044017231813107724	0.0
16-17	3.041521611604228E-4	8.690061747440652E-5	0.0	6.207186962457609E-6	0.049547835396657455	0.0
18-19	3.620859061433605E-4	8.690061747440652E-5	0.0	8.276249283276813E-6	0.05725302347938817	0.0
20-21	3.9519090327646775E-4	9.103874211604493E-5	0.0	8.276249283276813E-6	0.06314571296908125	4.138124641638406E-6
22-23	4.324340250512135E-4	1.0759124068259856E-4	0.0	8.276249283276813E-6	0.06898253777611224	4.138124641638406E-6
24-25	4.882987077133319E-4	1.241437392491522E-4	0.0	8.276249283276813E-6	0.07438485949577117	4.138124641638406E-6
26-27	5.255418294880776E-4	1.365581131740674E-4	0.0	8.276249283276813E-6	0.07822090103856996	4.138124641638406E-6
28-29	6.207186962457609E-4	1.4069623781570582E-4	0.0	8.276249283276813E-6	0.08221419131775104	4.138124641638406E-6
30-31	7.924508688737547E-4	1.489724870989826E-4	4.138124641638406E-6	8.276249283276813E-6	0.08680957873229048	4.138124641638406E-6
32-33	9.331471066894606E-4	1.5931779870307863E-4	4.138124641638406E-6	8.276249283276813E-6	0.091347032401847	4.138124641638406E-6
34-35	0.0010324620980887824	1.6138686102389784E-4	4.138124641638406E-6	8.276249283276813E-6	0.09454994087447512	4.138124641638406E-6
36-37	0.0010862577184300817	1.6345592334471705E-4	6.207186962457609E-6	8.276249283276813E-6	0.0979742390154309	4.138124641638406E-6
38-39	0.001131777089488104	1.7380123494881305E-4	8.276249283276813E-6	8.276249283276813E-6	0.10133232716212046	6.207186962457609E-6
40-41	0.0011793655228669458	1.9035373351536668E-4	8.276249283276813E-6	8.276249283276813E-6	0.10399107224437315	1.0345311604096015E-5
42-43	0.0012083323953584147	2.0276810744028188E-4	8.276249283276813E-6	8.276249283276813E-6	0.10707397510239376	1.2414373924915218E-5
44-45	0.001253851766416437	2.1518248136519712E-4	8.276249283276813E-6	1.2414373924915218E-5	0.10952581395256451	1.4483436245734422E-5
46-47	0.0013345451969283858	2.5242560313994274E-4	8.276249283276813E-6	1.2414373924915218E-5	0.11190109749686496	1.6552498566553625E-5
48-49	0.0014007551911946006	2.5242560313994274E-4	1.0345311604096015E-5	1.2414373924915218E-5	0.11419154948601182	2.069062320819203E-5
50-51	0.001448343624573442	2.5863279010240035E-4	1.2414373924915218E-5	1.2414373924915218E-5	0.11625647368218939	2.069062320819203E-5
52-53	0.0014917939333106453	2.669090393856772E-4	1.2414373924915218E-5	1.2414373924915218E-5	0.11830898350444202	2.2759685529011233E-5
54-55	0.0015166226811604757	2.834615379522308E-4	1.2414373924915218E-5	1.2414373924915218E-5	0.12019389927870833	2.4828747849830436E-5
56-57	0.0015931779870307864	2.917377872355076E-4	1.2414373924915218E-5	1.2414373924915218E-5	0.12203950286887905	2.4828747849830436E-5
58-59	0.0016655951682594584	3.0208309883960363E-4	1.2414373924915218E-5	1.2414373924915218E-5	0.12391614239386206	2.4828747849830436E-5
60-61	0.0017566339103755033	3.1863559740615726E-4	1.2414373924915218E-5	1.2414373924915218E-5	0.12532517383433994	2.4828747849830436E-5
62-63	0.0017793935959045145	3.351880959727109E-4	1.2414373924915218E-5	1.2414373924915218E-5	0.12723698741877687	2.689781017064964E-5
64-65	0.0018021532814335257	3.600168438225413E-4	1.2414373924915218E-5	1.2414373924915218E-5	0.12892327321024455	2.8966872491468844E-5
66-67	0.0018890538989079324	3.8691465399319096E-4	1.2414373924915218E-5	1.6552498566553625E-5	0.13024126590860638	2.8966872491468844E-5
68-69	0.0019283660830034972	3.9312184095564856E-4	1.2414373924915218E-5	1.6552498566553625E-5	0.131470288927173	3.103593481228805E-5
70-71	0.001959402017815785	4.096743395222022E-4	1.2414373924915218E-5	1.6552498566553625E-5	0.13267241413556893	3.310499713310725E-5
72-73	0.001996645139590531	4.200196511262982E-4	1.2414373924915218E-5	1.6552498566553625E-5	0.13353728218567135	3.310499713310725E-5
74-75	0.002021473887440361	4.220887134471174E-4	1.2414373924915218E-5	1.6552498566553625E-5	0.1346835427114052	3.310499713310725E-5
76-77	0.002044233572969373	4.28295900409575E-4	1.2414373924915218E-5	1.6552498566553625E-5	0.13587946073283869	3.310499713310725E-5
78-79	0.0020959601309898527	4.4277933665530945E-4	1.2414373924915218E-5	1.6552498566553625E-5	0.13731538998348725	3.310499713310725E-5
80-81	0.002120788878839683	4.4898652361776705E-4	1.2414373924915218E-5	1.6552498566553625E-5	0.13857131081222449	3.310499713310725E-5
82-83	0.0021352723150854175	4.6760808450513986E-4	1.8621560887372826E-5	1.6552498566553625E-5	0.14013966005140543	3.310499713310725E-5
84-85	0.002168377312218525	4.758843337884167E-4	2.4828747849830436E-5	1.6552498566553625E-5	0.1415155864947502	3.310499713310725E-5
86-87	0.0021787226238226204	4.841605830716935E-4	2.4828747849830436E-5	1.6552498566553625E-5	0.1429556538700404	3.310499713310725E-5
88-89	0.0021952751223891743	4.841605830716935E-4	2.4828747849830436E-5	1.6552498566553625E-5	0.1444764146758425	3.310499713310725E-5
90-91	0.0022138966832765472	5.007130816382471E-4	2.4828747849830436E-5	1.6552498566553625E-5	0.14539094022164456	3.724312177474565E-5
92-93	0.002222172932559824	5.007130816382471E-4	2.8966872491468844E-5	2.069062320819203E-5	0.14644616200526236	3.724312177474565E-5
94-95	0.0022387254311263777	5.007130816382471E-4	2.8966872491468844E-5	2.069062320819203E-5	0.14752414347440918	3.724312177474565E-5
96-97	0.002253208867372112	5.027821439590663E-4	2.8966872491468844E-5	2.4828747849830436E-5	0.14849039557823174	3.724312177474565E-5
98-99	0.002265623241297027	5.048512062798855E-4	3.103593481228805E-5	2.4828747849830436E-5	0.1494007829993922	3.9312184095564855E-5
100-101	0.0022780376152219425	5.110583932423432E-4	3.310499713310725E-5	2.4828747849830436E-5	0.1502635819871738	4.345030873720326E-5
102-103	0.002292521051467677	5.131274555631623E-4	3.310499713310725E-5	2.8966872491468844E-5	0.15108706879085984	4.965749569966087E-5
104-105	0.002307004487713411	5.317490164505351E-4	3.310499713310725E-5	2.8966872491468844E-5	0.15196642027720803	4.965749569966087E-5
106-107	0.002321487923959146	5.462324526962696E-4	3.310499713310725E-5	3.103593481228805E-5	0.15272576614894867	4.965749569966087E-5
108-109	0.0023256260486007843	5.545087019795464E-4	3.724312177474565E-5	3.310499713310725E-5	0.15355339107727634	4.965749569966087E-5
110-111	0.002342178547167338	5.689921382252809E-4	4.138124641638406E-5	3.310499713310725E-5	0.15435618725775418	5.379562034129928E-5
112-113	0.0023504547964506147	5.896827614334728E-4	4.138124641638406E-5	3.310499713310725E-5	0.15506587563379515	5.379562034129928E-5
114-115	0.0023504547964506147	6.020971353583881E-4	4.138124641638406E-5	3.310499713310725E-5	0.15585418837802728	5.793374498293769E-5
116-117	0.0023504547964506147	6.062352600000265E-4	4.138124641638406E-5	3.310499713310725E-5	0.15668388236867578	7.034811890785291E-5
118-119	0.0023545929210922532	6.145115092833033E-4	4.138124641638406E-5	3.310499713310725E-5	0.15741426136792497	7.655530587031051E-5
120-121	0.0023587310457338913	6.165805716041225E-4	4.138124641638406E-5	3.310499713310725E-5	0.15827085316874412	8.483155515358733E-5
122-123	0.0023587310457338913	6.186496339249417E-4	4.138124641638406E-5	3.310499713310725E-5	0.15896812717086017	8.690061747440652E-5
124-125	0.0023608001080547107	6.227877585665801E-4	4.138124641638406E-5	3.310499713310725E-5	0.1598309261586418	9.517686675768334E-5
126-127	0.0023690763573379877	6.289949455290377E-4	4.138124641638406E-5	3.310499713310725E-5	0.16082821419727666	9.517686675768334E-5
128-129	0.00238769791822536	6.41409319453953E-4	4.138124641638406E-5	3.310499713310725E-5	0.16170756568362482	9.724592907850254E-5
130-131	0.0024001122921502756	6.538236933788682E-4	4.345030873720326E-5	3.310499713310725E-5	0.16263864372799347	1.0345311604096015E-4
132-133	0.0024332172892833825	6.620999426621449E-4	4.5519371058022466E-5	3.310499713310725E-5	0.16338557522580918	1.0345311604096015E-4
134-135	0.0024828747849830438	6.641690049829642E-4	4.5519371058022466E-5	3.5174059453926454E-5	0.16409112547720855	1.0966030300341777E-4
136-137	0.0024870129096246823	6.662380673037834E-4	4.5519371058022466E-5	3.724312177474565E-5	0.1649270266548195	1.1586748996587537E-4
138-139	0.0024911510342663203	6.82790565870337E-4	4.5519371058022466E-5	3.724312177474565E-5	0.16570292502512668	1.2000561460751378E-4
>>END_MODULE
