##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.2d.R.3.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23338379
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	71-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.36583410527355	36.0	34.0	38.0	31.0	39.0
2	34.69077428213845	35.0	34.0	37.0	31.0	39.0
3	34.85895957041404	35.0	34.0	38.0	31.0	39.0
4	34.81825944295446	35.0	34.0	38.0	31.0	39.0
5	34.700524316620275	35.0	34.0	37.0	31.0	39.0
6	34.68871505600282	35.0	34.0	37.0	31.0	39.0
7	34.68470865093072	35.0	34.0	37.0	32.0	39.0
8	34.82774750551442	35.0	34.0	37.0	31.0	39.0
9	34.805787497066525	35.0	34.0	37.0	31.0	39.0
10-14	34.78434604219942	35.0	34.0	37.0	31.0	39.0
15-19	34.787575298181594	35.0	34.0	37.0	31.0	39.0
20-24	34.792245811073684	35.0	34.0	37.0	31.0	39.0
25-29	34.78247844034069	35.0	34.0	37.0	31.0	39.0
30-34	34.769624171413106	35.0	34.0	37.0	31.0	39.0
35-39	34.766109531428896	35.0	34.0	37.0	31.0	39.0
40-44	34.696575259147174	35.0	34.0	37.0	31.0	39.0
45-49	34.61093152185077	35.0	34.0	37.0	31.0	39.0
50-54	34.569613596556984	35.0	34.0	37.0	30.2	39.0
55-59	34.56250178300729	35.0	34.0	37.0	30.0	39.0
60-64	34.53537572596623	35.0	34.0	37.0	30.0	39.0
65-69	34.51684342772906	35.0	34.0	37.0	30.0	39.0
70-74	34.49000843133647	35.0	34.0	37.0	30.0	39.0
75-79	34.472686487505506	35.0	34.0	37.0	30.0	39.0
80-84	34.44950780891655	35.0	34.0	37.0	30.0	39.0
85-89	34.429074118336715	35.0	34.0	37.0	30.0	39.0
90-94	34.402999243384194	35.0	34.0	37.0	30.0	39.0
95-99	34.37483049524188	35.0	34.0	37.0	30.0	39.0
100-104	34.337370481363166	35.0	34.0	37.0	30.0	39.0
105-109	34.29014874418557	35.0	34.0	37.0	30.0	39.0
110-114	34.255840332544814	35.0	34.0	37.0	30.0	39.0
115-119	34.21186410570631	35.0	34.0	37.0	30.0	39.0
120-124	34.175254402616886	35.0	34.0	37.0	29.0	39.0
125-129	34.1318202166389	35.0	34.0	37.0	29.0	39.0
130-134	34.077546883613294	35.0	34.0	37.0	29.0	39.0
135-139	34.03535402232412	35.0	34.0	37.0	29.0	39.0
140-144	34.00211855626805	35.0	34.0	37.0	29.0	39.0
145-149	34.01422125956222	35.0	34.0	37.0	29.0	39.0
150	34.93681951396954	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	10.0
22	291.0
23	3260.0
24	22267.0
25	87569.0
26	218838.0
27	391477.0
28	571884.0
29	741334.0
30	920218.0
31	1145817.0
32	1455623.0
33	1904992.0
34	2613041.0
35	4052100.0
36	8220547.0
37	989104.0
38	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.296590221625934	11.063566154273182	15.716952749803232	37.92289087429766
2	22.2852177635972	12.969823555543977	36.54556605920833	28.19939262165049
3	21.41119752058017	17.523019930746642	28.147141254080243	32.918641294592945
4	26.28209907393696	25.17652905293121	22.30492723398654	26.23644463914529
5	25.672291966209126	30.707124730036867	24.546982111691424	19.073601192062583
6	19.22378913778348	36.317337614584474	25.92376607274148	18.535107174890562
7	16.07095961603104	24.5649038095889	40.2999527172985	19.064183857081556
8	18.59799019973101	24.36737678718267	31.51384080932238	25.520792203763943
9	19.658635664416536	23.29150618152124	32.531469985301875	24.518388168760353
10-14	20.29926904370325	29.140150596030196	27.151092338179623	23.409488022086926
15-19	20.436291574406308	27.861953000451518	27.96775504753114	23.73400037761104
20-24	20.582492507776735	28.016730483850154	27.67762399891735	23.723153009455757
25-29	20.576748039419776	28.208119503090355	27.561220495659853	23.653911961830016
30-34	20.53906720306905	27.94372651126087	27.600110606875226	23.91709567879485
35-39	20.617609399946154	27.91099884548651	27.649253075508295	23.822138679059037
40-44	20.662439488298475	27.977408467721443	27.557452925002508	23.80269911897758
45-49	20.74437602190508	27.771502549623577	27.530105534067374	23.954015894403973
50-54	20.871388580532305	27.74875031065967	27.60515165149427	23.774709457313755
55-59	20.862470227240905	27.77177962091843	27.54110966975956	23.824640482081104
60-64	20.805137328583342	27.694175231313313	27.52986951822359	23.970817921879757
65-69	20.9294927821401	27.601359255652476	27.616073264761994	23.853074697445436
70-74	21.021339210757777	27.545678657796735	27.609379968764813	23.823602162680675
75-79	21.0381369104334	27.45151587460771	27.585803238786493	23.924543976172398
80-84	21.09813281193099	27.441976708852362	27.571317794561125	23.888572684655525
85-89	21.071996535404445	27.43240723079384	27.63872089334133	23.85687534046039
90-94	21.064580584033244	27.354317613851887	27.592604071144677	23.98849773097019
95-99	21.153835688978724	27.270113449980453	27.68452569387877	23.89152516716205
100-104	21.192585459426443	27.33248910340948	27.56384706612391	23.911078371040166
105-109	21.16961243344693	27.169734209337037	27.641940147878437	24.018713209337598
110-114	21.251820789938552	27.163044628754413	27.688554944199524	23.89657963710751
115-119	21.28369186047757	27.16635508091874	27.665109666328302	23.884843392275386
120-124	21.23929413102426	27.08742088151299	27.663214876472036	24.010070110990714
125-129	21.28263508761919	26.99754181516527	27.733560918377435	23.9862621788381
130-134	21.362316103039806	27.095699224324406	27.624567080079782	23.91741759255601
135-139	21.352928786421405	26.946374417631453	27.717488123356386	23.98320867259076
140-144	21.400955579722623	26.8722312316381	27.853883568999088	23.87292961964019
145-149	21.003175988371503	27.08853885083679	27.913734408056577	23.99455075273513
150	20.894111821977287	25.78777331690741	28.602065808880972	24.71604905223433
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	2.0
11	5.0
12	10.0
13	20.5
14	37.0
15	70.0
16	148.0
17	292.5
18	579.0
19	1051.0
20	1970.5
21	3371.0
22	5318.5
23	8553.5
24	13651.5
25	21636.0
26	32416.0
27	45523.0
28	62116.0
29	87572.0
30	119007.0
31	150932.0
32	182832.5
33	219646.0
34	259106.0
35	304092.0
36	373213.0
37	467569.5
38	581531.0
39	722153.5
40	884154.5
41	1045800.0
42	1210758.5
43	1381303.5
44	1522166.5
45	1606142.0
46	1629539.0
47	1592294.5
48	1509060.5
49	1385729.0
50	1224147.0
51	1048535.5
52	871022.0
53	696724.5
54	538982.5
55	405615.0
56	300934.5
57	223973.0
58	168859.0
59	126874.5
60	89727.0
61	63967.5
62	46592.5
63	30131.0
64	19410.0
65	14891.5
66	14144.0
67	10557.5
68	5096.0
69	2543.5
70	1903.5
71	1274.5
72	585.5
73	237.5
74	109.0
75	74.5
76	50.5
77	39.0
78	17.0
79	1.5
80	0.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.8424587243184284E-4
3	6.855660369556943E-5
4	4.713266504070398E-5
5	2.9993514116811625E-5
6	1.2854363192919268E-4
7	1.4568278285308504E-4
8	1.7139150923892356E-4
9	1.2854363192919268E-4
10-14	2.0566981108670828E-4
15-19	3.350704005620956E-4
20-24	1.979571931709567E-4
25-29	3.590652118555449E-4
30-34	9.752176875694752E-4
35-39	0.0010146377346944276
40-44	0.0012408745268898066
45-49	9.769316026618643E-4
50-54	5.947285370590647E-4
55-59	4.2248007027394664E-4
60-64	2.3480636765732527E-4
65-69	3.265008251001494E-4
70-74	3.5478043932616054E-4
75-79	2.7079866349890506E-4
80-84	2.913658054067707E-4
85-89	3.727771906703656E-4
90-94	3.770625869820187E-4
95-99	3.273597576015828E-4
100-104	4.507643008659405E-4
105-109	2.7423066835541337E-4
110-114	2.442382566989669E-4
115-119	2.3138562625633826E-4
120-124	2.1510546136569223E-4
125-129	1.208381332925169E-4
130-134	1.6540725326917794E-4
135-139	1.637046748466893E-4
140-144	1.6806077221575414E-4
145-149	1.5211277165319404E-4
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
70-71	1.0
72-73	2.0
74-75	0.0
76-77	6.0
78-79	4.0
80-81	6.0
82-83	7.0
84-85	10.0
86-87	10.0
88-89	10.0
90-91	20.0
92-93	27.0
94-95	25.0
96-97	24.0
98-99	44.0
100-101	46.0
102-103	42.0
104-105	44.0
106-107	63.0
108-109	54.0
110-111	62.0
112-113	76.0
114-115	76.0
116-117	105.0
118-119	97.0
120-121	125.0
122-123	141.0
124-125	145.0
126-127	190.0
128-129	230.0
130-131	324.0
132-133	430.0
134-135	615.0
136-137	1005.0
138-139	2728.0
140-141	5762.0
142-143	9831.0
144-145	74180.0
146-147	747525.0
148-149	1425155.0
150-151	2.1069132E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.830431088102245
#Duplication Level	Percentage of total
1	39.16533119970963
2	10.955107302488019
3	6.431572172942612
4	4.556148595607966
5	3.3690391908321375
6	2.6908720929250194
7	2.11797118255555
8	1.8918325856192388
9	1.6303147528777737
>10	17.75040592007007
>50	4.441062325396736
>100	4.316591307329153
>500	0.3845929740649169
>1k	0.2991583975811588
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	2.5708726385838535E-5	0.0	0.0	2.1852417427962756E-4	1.7139150923892358E-5
2	0.0	9.426533008140797E-5	0.0	1.2854363192919268E-5	0.0019538632053237286	3.8563089578757806E-5
3	0.0	1.242588441982196E-4	0.0	1.2854363192919268E-5	0.0031107558926864627	7.284139142654252E-5
4	2.9993514116811625E-5	1.4996757058405812E-4	4.2847877309730894E-6	1.2854363192919268E-5	0.004511881480714663	8.569575461946179E-5
5	3.4278301847784716E-5	1.5425235831503122E-4	4.2847877309730894E-6	1.7139150923892358E-5	0.005737330771772966	9.855011781238106E-5
6	4.2847877309730896E-5	1.585371460460043E-4	4.2847877309730894E-6	1.7139150923892358E-5	0.006761395039475535	1.0283490554335414E-4
7	4.7132665040703986E-5	1.9281544789378902E-4	4.2847877309730894E-6	1.7139150923892358E-5	0.008038261783305516	1.0283490554335414E-4
8	5.998702823362325E-5	2.2280896201060064E-4	4.2847877309730894E-6	1.7139150923892358E-5	0.010184940436523033	1.199740564672465E-4
9	7.712617915751561E-5	2.2280896201060064E-4	4.2847877309730894E-6	1.7139150923892358E-5	0.013484226989372312	1.2854363192919268E-4
10-11	1.3711320739113886E-4	2.2709374974157375E-4	4.2847877309730894E-6	2.356633252035199E-5	0.018066807467648032	1.8638826629732938E-4
12-13	2.0566981108670828E-4	2.2923614360706028E-4	4.2847877309730894E-6	2.9993514116811625E-5	0.021721731402168078	2.2923614360706028E-4
14-15	2.2280896201060064E-4	2.485176883964392E-4	4.2847877309730894E-6	2.9993514116811625E-5	0.026130777977339384	2.63514445454845E-4
16-17	2.8065359637873737E-4	2.5922965772387193E-4	4.2847877309730894E-6	2.9993514116811625E-5	0.030717643243346075	2.6994162705130465E-4
18-19	3.3635583688138753E-4	2.7636880864776424E-4	4.2847877309730894E-6	2.9993514116811625E-5	0.0348224698896183	2.742264147822777E-4
20-21	3.684917448636857E-4	3.1707429209200865E-4	4.2847877309730894E-6	2.9993514116811625E-5	0.03862093421312594	2.785112025132508E-4
22-23	4.134820160389031E-4	3.277862614194414E-4	4.2847877309730894E-6	3.213590798229817E-5	0.04266791622503002	2.977927473026297E-4
24-25	4.777538320034995E-4	3.3849823074687404E-4	4.2847877309730894E-6	3.4278301847784716E-5	0.04721836079532345	2.9993514116811624E-4
26-27	5.377408602371227E-4	3.3849823074687404E-4	4.2847877309730894E-6	4.070548344424435E-5	0.05170239115578679	3.127895043610355E-4
28-29	5.78446343681367E-4	3.3849823074687404E-4	4.2847877309730894E-6	4.499027117521744E-5	0.05612429209415101	3.3421344301590096E-4
30-31	6.320061903185307E-4	3.3849823074687404E-4	4.2847877309730894E-6	5.570224050265016E-5	0.06031909928277367	3.5992216940173953E-4
32-33	6.684268860318019E-4	3.3849823074687404E-4	4.2847877309730894E-6	5.570224050265016E-5	0.06422682569342113	4.027700467114704E-4
34-35	7.177019449379925E-4	3.3849823074687404E-4	4.2847877309730894E-6	5.7844634368136705E-5	0.06791174314205799	4.070548344424435E-4
36-37	7.348410958618848E-4	3.449254123433337E-4	4.2847877309730894E-6	5.998702823362325E-5	0.07150882244220988	4.1133962217341656E-4
38-39	7.669770038441831E-4	3.642069571327126E-4	4.2847877309730894E-6	5.998702823362325E-5	0.07587716353393695	4.1776680376987626E-4
40-41	8.1839445661586E-4	3.642069571327126E-4	4.2847877309730894E-6	5.998702823362325E-5	0.0791186054524181	4.220515915008493E-4
42-43	8.269640320778063E-4	3.684917448636857E-4	4.2847877309730894E-6	5.998702823362325E-5	0.08233005385678244	4.2419398536633584E-4
44-45	8.762390909839968E-4	3.8563089578757805E-4	4.2847877309730894E-6	6.21294220991098E-5	0.08674981240128117	4.2419398536633584E-4
46-47	9.3622611921762E-4	3.963428651150108E-4	4.2847877309730894E-6	6.641420983008288E-5	0.09104745449544718	4.28478773097309E-4
48-49	9.705044210654047E-4	4.0062765284598384E-4	4.2847877309730894E-6	7.284139142654252E-5	0.09493161457357428	4.434755301557147E-4
50-51	9.83358784258324E-4	4.027700467114704E-4	8.569575461946179E-6	7.284139142654252E-5	0.09938136663218984	4.499027117521744E-4
52-53	0.001026206661568055	4.027700467114704E-4	8.569575461946179E-6	7.284139142654252E-5	0.103415494280901	4.648994688105802E-4
54-55	0.0010583425695503532	4.027700467114704E-4	8.569575461946179E-6	7.284139142654252E-5	0.10689259952458566	4.7561143813801293E-4
56-57	0.001096905659129111	4.070548344424435E-4	8.569575461946179E-6	7.498378529202906E-5	0.1112630830101782	4.841810135999591E-4
58-59	0.0011440383241698147	4.1990919763536277E-4	8.569575461946179E-6	9.212293621592142E-5	0.1155007380761106	4.863234074654456E-4
60-61	0.001204025352403438	4.2419398536633584E-4	8.569575461946179E-6	9.855011781238106E-5	0.11902069119710498	4.927505890619053E-4
62-63	0.0012618699867715747	4.2419398536633584E-4	1.2854363192919268E-5	9.855011781238106E-5	0.12244852138188346	4.948929829273919E-4
64-65	0.0013025754702158192	4.2419398536633584E-4	1.2854363192919268E-5	9.855011781238106E-5	0.1276652504443432	4.970353767928784E-4
66-67	0.0013090026518122787	4.3704834855925513E-4	1.2854363192919268E-5	9.855011781238106E-5	0.13189647832867912	5.013201645238516E-4
68-69	0.0013347113781981175	4.5847228721412057E-4	1.2854363192919268E-5	9.855011781238106E-5	0.13470515668633198	5.120321338512842E-4
70-71	0.0013775592555078484	4.8203861973447257E-4	1.2854363192919268E-5	9.855011781238106E-5	0.1374259968954999	5.141745277167707E-4
72-73	0.0014268343144140387	4.884658013309322E-4	1.2854363192919268E-5	9.855011781238106E-5	0.14004185980525896	5.184593154477439E-4
74-75	0.0014696821917237696	4.906081951964187E-4	1.2854363192919268E-5	9.855011781238106E-5	0.14312262218382862	5.227441031787169E-4
76-77	0.0015018180997060679	5.03462558389338E-4	1.2854363192919268E-5	1.0497729940884069E-4	0.1455885175230036	5.227441031787169E-4
78-79	0.0015446659770157987	5.098897399857976E-4	1.2854363192919268E-5	1.4354038898759848E-4	0.14821080761435917	5.248864970442034E-4
80-81	0.0015746594911326102	5.141745277167707E-4	1.2854363192919268E-5	1.4996757058405812E-4	0.1509145086726032	5.2702889090969E-4
82-83	0.001617507368442341	5.141745277167707E-4	1.2854363192919268E-5	1.4996757058405812E-4	0.1534125399197605	5.31313678640663E-4
84-85	0.0016496432764246394	5.227441031787169E-4	1.2854363192919268E-5	1.4996757058405812E-4	0.15586986568347355	5.355984663716362E-4
86-87	0.0017331966371786148	5.2702889090969E-4	1.2854363192919268E-5	1.4996757058405812E-4	0.1580765313649247	5.355984663716362E-4
88-89	0.001778186908353832	5.2702889090969E-4	1.2854363192919268E-5	1.4996757058405812E-4	0.1601932165040254	5.355984663716362E-4
90-91	0.0018188923917980765	5.2702889090969E-4	1.2854363192919268E-5	1.4996757058405812E-4	0.1631411504629349	5.355984663716362E-4
92-93	0.0018810218138971863	5.291712847751766E-4	1.2854363192919268E-5	1.4996757058405812E-4	0.16576129816042495	5.355984663716362E-4
94-95	0.0019410088421308095	5.398832541026093E-4	1.2854363192919268E-5	1.4996757058405812E-4	0.16878207351076097	5.441680418335823E-4
96-97	0.0020288469906157578	5.570224050265016E-4	1.2854363192919268E-5	1.4996757058405812E-4	0.17117084267077848	5.441680418335823E-4
98-99	0.0020931188065803543	5.741615559503939E-4	1.2854363192919268E-5	1.5425235831503122E-4	0.17319754726752873	5.441680418335823E-4
100-101	0.0021873841366617623	5.913007068742863E-4	1.2854363192919268E-5	1.5425235831503122E-4	0.17535922267780465	5.463104356990689E-4
102-103	0.002206665681451141	5.913007068742863E-4	1.2854363192919268E-5	1.5425235831503122E-4	0.17716097591867885	5.484528295645554E-4
104-105	0.002240943983298926	5.998702823362325E-4	1.2854363192919268E-5	1.585371460460043E-4	0.17883847031535482	5.527376172955286E-4
106-107	0.0022795070728776835	5.998702823362325E-4	1.2854363192919268E-5	1.585371460460043E-4	0.18037242432304318	5.570224050265016E-4
108-109	0.0023244973440529008	5.998702823362325E-4	1.2854363192919268E-5	1.585371460460043E-4	0.18182710975770855	5.570224050265016E-4
110-111	0.0023780571906900647	6.062974639326922E-4	1.2854363192919268E-5	1.585371460460043E-4	0.1832539440721226	5.570224050265016E-4
112-113	0.0024444714005201476	6.084398577981787E-4	1.2854363192919268E-5	1.585371460460043E-4	0.1849228688933366	5.570224050265016E-4
114-115	0.0024916040655608516	6.148670393946383E-4	1.2854363192919268E-5	1.6496432764246394E-4	0.18674604607286566	5.570224050265016E-4
116-117	0.002560160669256421	6.170094332601249E-4	1.2854363192919268E-5	1.7567629696989666E-4	0.18827357289895755	5.570224050265016E-4
118-119	0.002622290091355531	6.470029473769365E-4	1.2854363192919268E-5	1.778186908353832E-4	0.18976896381706715	5.570224050265016E-4
120-121	0.002716555421436939	6.641420983008288E-4	1.2854363192919268E-5	1.7996108470086977E-4	0.19121722207013606	5.570224050265016E-4
122-123	0.0028686653858864836	6.684268860318019E-4	1.2854363192919268E-5	1.821034785663563E-4	0.19260763568883682	5.570224050265016E-4
124-125	0.0029629307159678916	6.684268860318019E-4	1.2854363192919268E-5	1.8424587243184284E-4	0.1940773178805606	5.570224050265016E-4
126-127	0.0030164905626050547	6.705692798972885E-4	1.2854363192919268E-5	1.8424587243184284E-4	0.19548487065018527	5.570224050265016E-4
128-129	0.0030743351969731918	6.769964614937481E-4	1.2854363192919268E-5	1.9067305402830248E-4	0.19673174387989842	5.613071927574747E-4
130-131	0.003138607012937788	6.855660369556942E-4	1.2854363192919268E-5	1.9710023562476213E-4	0.19799147147280452	5.613071927574747E-4
132-133	0.0032457267062121153	6.898508246866674E-4	1.2854363192919268E-5	1.9710023562476213E-4	0.1990905195257991	5.613071927574747E-4
134-135	0.0034106910338545793	6.898508246866674E-4	1.2854363192919268E-5	1.9710023562476213E-4	0.20006102394686454	5.613071927574747E-4
136-137	0.0035542314228421776	6.941356124176405E-4	1.7139150923892358E-5	1.9710023562476213E-4	0.20116221439372461	5.634495866229613E-4
138-139	0.0035692281799005834	7.069899756105597E-4	1.7139150923892358E-5	1.9924262949024866E-4	0.2022484080835263	5.655919804884478E-4
>>END_MODULE
