##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.2d.R.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23338379
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	62-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.97106761356476	37.0	35.0	38.0	33.0	39.0
2	35.323368988051826	35.0	34.0	37.0	33.0	39.0
3	35.46213432389627	35.0	34.0	37.0	33.0	39.0
4	35.51286788169821	36.0	35.0	38.0	33.0	39.0
5	35.42530691613158	36.0	35.0	37.0	33.0	39.0
6	35.38233225195289	35.0	35.0	37.0	33.0	39.0
7	35.41187534918342	35.0	34.0	37.0	33.0	39.0
8	35.49646245782537	36.0	35.0	38.0	33.0	39.0
9	35.500168756364786	36.0	35.0	38.0	33.0	39.0
10-14	35.48380236690817	35.8	34.6	37.8	33.0	39.0
15-19	35.45673981042128	36.0	35.0	38.0	33.0	39.0
20-24	35.441672397213196	36.0	34.2	38.0	33.0	39.0
25-29	35.42105908897956	36.0	34.0	38.0	33.0	39.0
30-34	35.40084567998488	36.0	34.0	38.0	33.0	39.0
35-39	35.37347164513868	36.0	34.0	38.0	33.0	39.0
40-44	35.35491809435437	36.0	34.0	38.0	33.0	39.0
45-49	35.32512165476446	36.0	34.0	38.0	32.0	39.0
50-54	35.302266742690236	36.0	34.0	38.0	32.0	39.0
55-59	35.27483539452333	36.0	34.0	38.0	32.0	39.0
60-64	35.233701976162536	36.0	34.0	38.0	32.0	39.0
65-69	35.20517788515251	36.0	34.0	38.0	32.0	39.0
70-74	35.17435736475498	36.0	34.0	37.8	32.0	39.0
75-79	35.12695479147798	35.6	34.0	37.2	32.0	39.0
80-84	35.08481833491835	35.4	34.0	37.0	32.0	39.0
85-89	35.03528854764226	35.0	34.0	37.0	32.0	39.0
90-94	34.97808452105362	35.0	34.0	37.0	31.6	39.0
95-99	34.91427093460613	35.0	34.0	37.0	31.0	39.0
100-104	34.864561681162044	35.0	34.0	37.0	31.0	39.0
105-109	34.80193964943296	35.0	34.0	37.0	31.0	39.0
110-114	34.72761358383355	35.0	34.0	37.0	31.0	39.0
115-119	34.666019759109325	35.0	34.0	37.0	30.4	39.0
120-124	34.59087364785421	35.0	34.0	37.0	30.0	39.0
125-129	34.514174739388935	35.0	34.0	37.0	30.0	39.0
130-134	34.412334124139974	35.0	34.0	37.0	30.0	39.0
135-139	34.353659264219765	35.0	34.0	37.0	29.8	39.0
140-144	34.22949219829463	35.0	34.0	37.0	29.0	39.0
145-149	34.22308927341873	35.0	34.0	37.0	29.0	39.0
150	35.018805661811875	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	10.0
21	143.0
22	1480.0
23	7980.0
24	28179.0
25	70336.0
26	141906.0
27	245516.0
28	365388.0
29	487082.0
30	626139.0
31	818088.0
32	1093386.0
33	1527575.0
34	2292382.0
35	3906567.0
36	1.010261E7
37	1623605.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.08061031145308	18.597037094992757	16.172914151407003	31.149438442147158
2	24.416005927403955	25.47302449754544	32.9353465379922	17.1756230370584
3	20.156691259491502	29.38075519298063	29.600333425042074	20.8622201224858
4	23.816993623121537	33.90934451400179	22.151907900369082	20.12175396250759
5	25.348218405998363	35.107584634231095	22.309497192820235	17.234699766950307
6	19.11804428417421	38.860151270269206	22.759401260414187	19.2624031851424
7	20.494906275145595	20.67091661852238	37.72921847542572	21.104958630906307
8	21.793606617551962	23.32235449797298	28.165909633064736	26.71812925141033
9	23.66152336458329	23.286077409232234	28.553534073638964	24.498865152545513
10-14	23.307217037714718	29.136517400257468	25.472347719371154	22.083917842656664
15-19	23.115125519214512	28.32191387413839	26.948196359310128	21.614764247336975
20-24	23.20233637477564	28.374509643536083	26.845420583837466	21.57773339785081
25-29	23.273367014907077	28.331103029906235	26.790343922343535	21.605186032843154
30-34	23.260415815511436	28.08064261875257	27.017746176801737	21.641195388934253
35-39	23.332256680419093	28.081429787071844	26.992033628248706	21.594279904260357
40-44	23.471984065388604	27.85638625544645	27.09819735123849	21.573432327926458
45-49	23.466630651597526	27.698318722135756	27.182166336402368	21.652884289864346
50-54	23.521110013681756	27.685910833824405	27.147996011205404	21.64498314128843
55-59	23.692869157707996	27.58655260504596	27.048689199879732	21.671889037366306
60-64	23.588286463053606	27.573570170808697	27.113834720292008	21.72430864584569
65-69	23.68960085813691	27.495021096971207	27.06261907588437	21.752758969007516
70-74	23.763716902130227	27.463734384078403	26.990751706632345	21.781797007159028
75-79	23.678425529024206	27.52582621871744	27.05623538985316	21.739512862405192
80-84	23.791469770224158	27.572429065477305	26.913475382198037	21.722625782100497
85-89	23.82846310770342	27.50665157830103	26.957017439745446	21.7078678742501
90-94	23.750377488778945	27.453200154026646	27.03582968933489	21.76059266785952
95-99	23.810946045434363	27.554266677063666	27.037866860332667	21.5969204171693
100-104	23.987180040276794	27.518809399066445	26.858022329518	21.635988231138757
105-109	23.770203120716367	27.64679561225541	26.97843931751897	21.60456194950925
110-114	23.801747972489423	27.63729728140895	26.919704857677097	21.641249888424525
115-119	23.87138731438593	27.6127465939034	26.952003727526332	21.563862364184345
120-124	23.819299181749408	27.53368978644192	27.135965042610678	21.511045989198003
125-129	23.792061084025807	27.62811311666794	27.093855028293394	21.48597077101286
130-134	23.862879883251523	27.52737681716497	27.163393686802685	21.44634961278082
135-139	23.74644375354729	27.538896312967143	27.267202538789583	21.447457394695988
140-144	23.790273034149884	27.656483797913673	27.237615029206086	21.315628138730357
145-149	23.53511446418608	27.782893321289144	27.16144644529676	21.52054576922802
150	23.668182672268284	26.543322976907874	27.597133459653104	22.19136089117074
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	3.0
9	3.0
10	5.0
11	8.0
12	7.5
13	8.5
14	17.0
15	33.5
16	54.0
17	84.5
18	145.5
19	260.0
20	423.0
21	653.0
22	1043.5
23	1857.5
24	3205.5
25	5313.0
26	8326.0
27	13320.0
28	22045.0
29	33433.0
30	46681.0
31	63678.0
32	87258.5
33	119652.5
34	163650.0
35	229845.5
36	325817.0
37	443871.0
38	583412.5
39	760121.0
40	971142.5
41	1174558.0
42	1357500.5
43	1522306.5
44	1641703.5
45	1700836.5
46	1706988.5
47	1657142.5
48	1557251.5
49	1417379.0
50	1234984.5
51	1035989.5
52	843892.0
53	670739.0
54	522925.0
55	394058.5
56	290391.0
57	214289.0
58	156005.5
59	112220.0
60	77873.5
61	51507.5
62	34405.5
63	23735.5
64	16354.5
65	11080.5
66	7742.0
67	5696.5
68	5067.5
69	3768.0
70	1814.0
71	1103.0
72	612.5
73	435.5
74	349.0
75	209.0
76	135.0
77	52.5
78	19.5
79	3.0
80	1.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	4.2847877309730894E-6
5	3.8563089578757806E-5
6	8.141096688848869E-5
7	0.002339494101111307
8	0.11378682298372136
9	0.0
10-14	8.569575461946178E-7
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	1.7139150923892357E-6
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	8.569898673117446E-7
100-104	0.0
105-109	5.05642056913493E-5
110-114	0.01895341561546844
115-119	0.0
120-124	1.71417560054278E-6
125-129	2.571367049635218E-6
130-134	0.04705111179134497
135-139	8.5727285644816E-7
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62-63	1.0
64-65	3.0
66-67	3.0
68-69	2.0
70-71	9.0
72-73	13.0
74-75	19.0
76-77	23.0
78-79	24.0
80-81	33.0
82-83	62.0
84-85	72.0
86-87	82.0
88-89	134.0
90-91	112.0
92-93	125.0
94-95	104.0
96-97	121.0
98-99	151.0
100-101	190.0
102-103	150.0
104-105	184.0
106-107	197.0
108-109	188.0
110-111	225.0
112-113	192.0
114-115	237.0
116-117	274.0
118-119	326.0
120-121	277.0
122-123	335.0
124-125	377.0
126-127	467.0
128-129	518.0
130-131	597.0
132-133	810.0
134-135	1060.0
136-137	1531.0
138-139	3447.0
140-141	6893.0
142-143	10734.0
144-145	68992.0
146-147	815005.0
148-149	1040254.0
150-151	2.1383826E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.6186175532314
#Duplication Level	Percentage of total
1	29.147813476525354
2	10.804061093188686
3	7.227326643294152
4	5.069954673611644
5	3.8770677488638947
6	3.154159746575632
7	2.552020935954725
8	2.060029170553058
9	1.8383302803539603
>10	20.962963590676047
>50	5.176934300536526
>100	6.147592778019758
>500	1.0382152759890204
>1k	0.834586047202117
>5k	0.10894423865531176
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.2847877309730894E-6	8.569575461946179E-6	0.0	0.0	5.955854946052595E-4	0.0
2	8.569575461946179E-6	2.1423938654865448E-5	0.0	4.2847877309730894E-6	0.004361913910130605	0.0
3	5.141745277167707E-5	2.1423938654865448E-5	0.0	4.2847877309730894E-6	0.0057244764085800474	0.0
4	9.855011781238106E-5	2.1423938654865448E-5	0.0	4.2847877309730894E-6	0.006962780062831271	0.0
5	9.855011781238106E-5	2.1423938654865448E-5	0.0	4.2847877309730894E-6	0.008325342561280712	0.0
6	1.0711969327432723E-4	2.1423938654865448E-5	0.0	8.569575461946179E-6	0.009683620271999182	0.0
7	1.1140448100530032E-4	2.5708726385838535E-5	4.2847877309730894E-6	8.569575461946179E-6	0.011436098453967175	0.0
8	1.4568278285308504E-4	2.9993514116811625E-5	4.2847877309730894E-6	8.569575461946179E-6	0.016299332528621632	0.0
9	1.585371460460043E-4	2.9993514116811625E-5	4.2847877309730894E-6	8.569575461946179E-6	0.021543912711332693	0.0
10-11	1.9924262949024866E-4	3.4278301847784716E-5	4.2847877309730894E-6	8.569575461946179E-6	0.02745477738621007	0.0
12-13	2.2280896201060064E-4	3.4278301847784716E-5	4.2847877309730894E-6	8.569575461946179E-6	0.03290074259227687	0.0
14-15	2.677992331858181E-4	4.2847877309730896E-5	4.2847877309730894E-6	8.569575461946179E-6	0.040223444824509874	0.0
16-17	2.9565035343714316E-4	4.7132665040703986E-5	4.2847877309730894E-6	8.569575461946179E-6	0.04544445867470058	0.0
18-19	3.534949878052799E-4	5.7844634368136705E-5	4.2847877309730894E-6	8.569575461946179E-6	0.05253578236946105	0.0
20-21	3.9420047124952425E-4	6.641420983008288E-5	4.2847877309730894E-6	8.569575461946179E-6	0.05769680919141813	0.0
22-23	4.263363792318224E-4	8.569575461946179E-5	4.2847877309730894E-6	8.569575461946179E-6	0.06316848312387077	0.0
24-25	4.906081951964187E-4	9.212293621592142E-5	4.2847877309730894E-6	8.569575461946179E-6	0.067748921208281	0.0
26-27	5.677343743539344E-4	9.855011781238106E-5	4.2847877309730894E-6	8.569575461946179E-6	0.07102464142860992	0.0
28-29	6.769964614937482E-4	1.0497729940884069E-4	4.2847877309730894E-6	8.569575461946179E-6	0.07436677585876894	0.0
30-31	8.312488198087794E-4	1.1140448100530032E-4	4.2847877309730894E-6	1.2854363192919268E-5	0.07865156358974204	0.0
32-33	9.640772394689451E-4	1.1354687487078686E-4	4.2847877309730894E-6	1.2854363192919268E-5	0.08301133510600715	0.0
34-35	0.001073339326608759	1.1783166260175996E-4	4.2847877309730894E-6	1.2854363192919268E-5	0.08572574813357861	0.0
36-37	0.0011354687487078689	1.2211645033273304E-4	4.2847877309730894E-6	1.2854363192919268E-5	0.08879579854282083	0.0
38-39	0.0011933133830760055	1.2854363192919268E-4	4.2847877309730894E-6	1.2854363192919268E-5	0.09188513049685242	0.0
40-41	0.0012340188665202498	1.3282841966016579E-4	8.569575461946179E-6	1.4996757058405813E-5	0.09436173780535487	0.0
42-43	0.0012447308358476824	1.4782517671857158E-4	8.569575461946179E-6	1.7139150923892358E-5	0.09694546480713163	0.0
44-45	0.0012811515315609538	1.6924911537343705E-4	8.569575461946179E-6	1.7139150923892358E-5	0.09900216291799871	0.0
46-47	0.0013197146211397115	2.0995459881768138E-4	8.569575461946179E-6	1.7139150923892358E-5	0.10135022659457196	0.0
48-49	0.0013797016493733347	2.16381780414141E-4	1.2854363192919268E-5	1.7139150923892358E-5	0.103415494280901	0.0
50-51	0.0014268343144140387	2.2280896201060064E-4	1.2854363192919268E-5	1.7139150923892358E-5	0.10529651609479818	0.0
52-53	0.0014675397978582833	2.2280896201060064E-4	1.2854363192919268E-5	1.7139150923892358E-5	0.10716468354550246	0.0
54-55	0.0014868213426476621	2.4637529453095264E-4	1.7139150923892358E-5	1.7139150923892358E-5	0.10887431385016072	2.1423938654865447E-6
56-57	0.0015189572506299602	2.549448699928988E-4	1.7139150923892358E-5	1.7139150923892358E-5	0.11065250075851454	4.2847877309730894E-6
58-59	0.00158108667272907	2.5922965772387193E-4	1.7139150923892358E-5	1.7139150923892358E-5	0.11237070063863476	4.2847877309730894E-6
60-61	0.0016624976396175588	2.7208402091679116E-4	2.1423938654865448E-5	1.7139150923892358E-5	0.11380181974077977	8.569575461946179E-6
62-63	0.0016924911537343702	2.827959902442239E-4	2.1423938654865448E-5	1.7139150923892358E-5	0.11555858271047874	8.569575461946179E-6
64-65	0.001718199880120209	3.1921668595749517E-4	2.1423938654865448E-5	1.7139150923892358E-5	0.11722964992555823	8.569575461946179E-6
66-67	0.001801753240874184	3.449254123433337E-4	2.1423938654865448E-5	1.7139150923892358E-5	0.1183844002190555	8.569575461946179E-6
68-69	0.001840316330452942	3.5992216940173953E-4	2.1423938654865448E-5	1.7139150923892358E-5	0.11957985599599698	8.569575461946179E-6
70-71	0.001848885905914888	3.5992216940173953E-4	2.1423938654865448E-5	1.9281544789378903E-5	0.1207388910772252	8.569575461946179E-6
72-73	0.0018681674507042669	3.749189264601453E-4	2.1423938654865448E-5	2.1423938654865448E-5	0.1216151301682092	8.569575461946179E-6
74-75	0.0018895913893591325	3.8348850192209153E-4	2.1423938654865448E-5	2.1423938654865448E-5	0.12259206177087106	8.569575461946179E-6
76-77	0.0019238696912069171	4.070548344424435E-4	2.785112025132508E-5	2.1423938654865448E-5	0.12377466318461963	8.569575461946179E-6
78-79	0.0019881415071715137	4.1133962217341656E-4	2.9993514116811625E-5	2.1423938654865448E-5	0.12531504437390445	8.569575461946179E-6
80-81	0.0019924262949024864	4.1990919763536277E-4	2.9993514116811625E-5	2.1423938654865448E-5	0.1266154774502548	8.569575461946179E-6
82-83	0.0019945686887679727	4.284787730973089E-4	2.9993514116811625E-5	2.1423938654865448E-5	0.12805302373399627	8.569575461946179E-6
84-85	0.0020074230519608926	4.3276356082828205E-4	2.9993514116811625E-5	2.1423938654865448E-5	0.12950342438093065	1.2854363192919268E-5
86-87	0.0020202774151538115	4.456179240212013E-4	2.9993514116811625E-5	2.1423938654865448E-5	0.13108022626592875	1.2854363192919268E-5
88-89	0.002037416566077704	4.541874994831475E-4	2.9993514116811625E-5	2.1423938654865448E-5	0.13266774012025428	1.2854363192919268E-5
90-91	0.0020459861415396503	4.6275707494509364E-4	2.9993514116811625E-5	2.1423938654865448E-5	0.13366395326770553	1.4996757058405813E-5
92-93	0.002050270929270623	4.6275707494509364E-4	3.213590798229817E-5	2.1423938654865448E-5	0.13452091081390016	1.7139150923892358E-5
94-95	0.002065267686329029	4.7561143813801293E-4	3.4278301847784716E-5	2.5708726385838535E-5	0.13547856087177262	2.1423938654865448E-5
96-97	0.0020695524740600023	4.8632340746544565E-4	3.4278301847784716E-5	2.5708726385838535E-5	0.1363719391136805	2.1423938654865448E-5
98-99	0.0021016883820423006	4.970353767928784E-4	3.4278301847784716E-5	2.5708726385838535E-5	0.13726531735558842	2.1423938654865448E-5
100-101	0.0021059731697732733	4.970353767928784E-4	3.4278301847784716E-5	2.5708726385838535E-5	0.13810513575085914	2.5708726385838535E-5
102-103	0.00210811556363876	4.970353767928784E-4	3.4278301847784716E-5	2.5708726385838535E-5	0.13900279878049798	2.5708726385838535E-5
104-105	0.002112400351369733	5.013201645238514E-4	3.4278301847784716E-5	2.5708726385838535E-5	0.13987903787148198	2.5708726385838535E-5
106-107	0.0021488210470830046	5.141745277167707E-4	3.4278301847784716E-5	2.5708726385838535E-5	0.14056460390843767	2.5708726385838535E-5
108-109	0.002155248228679464	5.2702889090969E-4	3.8563089578757806E-5	2.5708726385838535E-5	0.14129516021656857	2.5708726385838535E-5
110-111	0.0021681025918723834	5.355984663716362E-4	4.2847877309730896E-5	2.5708726385838535E-5	0.14217996888301454	2.5708726385838535E-5
112-113	0.0021766721673343293	5.398832541026093E-4	4.2847877309730896E-5	2.5708726385838535E-5	0.14294694588685872	2.5708726385838535E-5
114-115	0.0021809569550653024	5.463104356990689E-4	4.2847877309730896E-5	2.5708726385838535E-5	0.1437631979496091	2.5708726385838535E-5
116-117	0.0021873841366617623	5.484528295645554E-4	4.2847877309730896E-5	2.5708726385838535E-5	0.14465014900992051	2.5708726385838535E-5
118-119	0.0022002384998546813	5.484528295645554E-4	4.2847877309730896E-5	2.5708726385838535E-5	0.14533785744074168	2.9993514116811625E-5
120-121	0.0022152352569130875	5.527376172955286E-4	4.2847877309730896E-5	2.5708726385838535E-5	0.1461990997746673	2.9993514116811625E-5
122-123	0.0022216624385095465	5.570224050265016E-4	4.2847877309730896E-5	2.5708726385838535E-5	0.14681182442019644	2.9993514116811625E-5
124-125	0.0022366591955679527	5.677343743539343E-4	4.7132665040703986E-5	2.5708726385838535E-5	0.14763664605840876	2.9993514116811625E-5
126-127	0.002258083134222818	5.763039498158805E-4	4.7132665040703986E-5	2.5708726385838535E-5	0.1485557330267025	3.4278301847784716E-5
128-129	0.0022752222851467104	5.870159191433133E-4	4.7132665040703986E-5	2.5708726385838535E-5	0.1493012860918918	3.8563089578757806E-5
130-131	0.00228807664833963	6.062974639326922E-4	4.7132665040703986E-5	2.5708726385838535E-5	0.15012182294237317	3.8563089578757806E-5
132-133	0.002315927768590955	6.084398577981787E-4	4.7132665040703986E-5	2.5708726385838535E-5	0.15097235330697134	4.070548344424435E-5
134-135	0.00234377888884228	6.105822516636653E-4	4.7132665040703986E-5	2.5708726385838535E-5	0.15172647594762256	4.7132665040703986E-5
136-137	0.0023502060704387396	6.127246455291518E-4	4.7132665040703986E-5	2.5708726385838535E-5	0.15254701279810395	4.927505890619053E-5
138-139	0.002352348464304226	6.191518271256114E-4	4.7132665040703986E-5	2.5708726385838535E-5	0.15328613868169677	5.141745277167707E-5
>>END_MODULE
