##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.2d.S.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24190034
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	62-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.13219816061441	37.0	35.0	38.0	33.0	39.0
2	35.50001360064231	35.0	34.0	38.0	33.0	39.0
3	35.59335604902416	35.0	35.0	38.0	33.0	39.0
4	35.67678945800572	36.0	35.0	38.0	33.0	39.0
5	35.60511791756886	36.0	35.0	38.0	33.0	39.0
6	35.54399754047473	35.0	35.0	37.0	33.0	39.0
7	35.56916340010105	35.0	34.0	38.0	33.0	39.0
8	35.685228883927984	36.0	35.0	38.0	33.0	39.0
9	35.67278028629476	36.0	35.0	38.0	33.0	39.0
10-14	35.64326222939579	35.8	35.0	37.8	33.0	39.0
15-19	35.620511091468494	36.0	35.0	38.0	33.0	39.0
20-24	35.609375993435975	36.0	35.0	38.0	33.0	39.0
25-29	35.593078827421245	36.0	35.0	38.0	33.0	39.0
30-34	35.578360311523326	36.0	35.0	38.0	33.0	39.0
35-39	35.54942559402769	36.0	35.0	38.0	33.0	39.0
40-44	35.53643663336728	36.0	35.0	38.0	33.0	39.0
45-49	35.506564844018	36.0	35.0	38.0	33.0	39.0
50-54	35.48846222374057	36.0	35.0	38.0	33.0	39.0
55-59	35.46230307076046	36.0	34.8	38.0	33.0	39.0
60-64	35.421606061703386	36.0	34.0	38.0	33.0	39.0
65-69	35.394370723815236	36.0	34.0	38.0	32.6	39.0
70-74	35.36222701931117	36.0	34.0	38.0	32.0	39.0
75-79	35.31161053150013	36.0	34.0	38.0	32.0	39.0
80-84	35.27195167521459	36.0	34.0	38.0	32.0	39.0
85-89	35.22237101416166	36.0	34.0	38.0	32.0	39.0
90-94	35.17094158794747	36.0	34.0	38.0	32.0	39.0
95-99	35.102296407075556	36.0	34.0	38.0	32.0	39.0
100-104	35.056453382756445	36.0	34.0	38.0	32.0	39.0
105-109	34.99860829437726	36.0	34.0	38.0	31.2	39.0
110-114	34.92160836133148	35.2	34.0	38.0	31.0	39.0
115-119	34.84691699431312	35.0	34.0	37.6	31.0	39.0
120-124	34.77248312176457	35.0	34.0	37.0	31.0	39.0
125-129	34.6944827003218	35.0	34.0	37.0	30.0	39.0
130-134	34.60565983580621	35.0	34.0	37.0	30.0	39.0
135-139	34.5301397307044	35.0	34.0	37.0	30.0	39.0
140-144	34.40428451173986	35.0	34.0	37.0	30.0	39.0
145-149	34.39304873289605	35.0	34.0	37.0	29.8	39.0
150	35.157528636784576	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	6.0
21	128.0
22	1163.0
23	7033.0
24	24894.0
25	62878.0
26	126070.0
27	222577.0
28	336265.0
29	449854.0
30	581744.0
31	764259.0
32	1031856.0
33	1451626.0
34	2204329.0
35	3798218.0
36	1.0604759E7
37	2522366.0
38	9.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.50989915929841	18.899113577103694	15.667981285185462	31.923005978412434
2	24.57696008199079	25.54461891206933	31.96403940564945	17.914381600290433
3	20.23259661396094	29.350516828541867	29.598342027960772	20.81854452953642
4	23.67387329840049	33.951576917998544	21.501098344880376	20.873451438720593
5	25.70470549979384	34.93960777401139	21.46555891570884	17.89012781048592
6	19.295094831201972	39.043822757752224	21.666133251404275	19.99494915964153
7	20.691628626896513	20.815336596881178	36.633503698258544	21.85953107796376
8	21.765926414158823	23.486816926342478	27.90492977397221	26.842326885526496
9	23.803811106673102	23.422046451030205	27.42143314060658	25.35270930169011
10-14	23.20778714077045	28.83492846682233	25.081471154608547	22.875813237798674
15-19	23.34347525100626	27.649632902541597	26.58701843908115	22.419873407370986
20-24	23.180987674510916	27.65389002760393	26.529756014398327	22.635366283486828
25-29	23.27538522682523	27.227783144083222	26.538143766147666	22.95868786294389
30-34	23.087111824646463	26.94006630995227	26.783969795164403	23.18885207023686
35-39	23.429512335534543	26.67076615105212	26.793892063152953	23.105829450260384
40-44	23.616801861460797	26.326678995159742	26.8382706696485	23.21824847373096
45-49	23.494137296375854	26.14397730900254	26.94856733148866	23.41331806313294
50-54	23.696971240304997	26.16685697920061	26.65719113912779	23.478980641366608
55-59	23.755859954558144	26.17337453928341	26.70867019037675	23.362095315781698
60-64	23.407630984026415	26.139967825427078	26.803668433102136	23.64873275744437
65-69	23.621433429746048	26.06074888928218	26.809488688407047	23.508328992564724
70-74	23.78209251275329	26.039192101769874	26.518109301820953	23.660606083655882
75-79	23.62521917206805	26.157230296890884	26.77554333767554	23.442007193365534
80-84	23.965691388682618	26.159132995252925	26.43798806458137	23.43718755148308
85-89	23.661441676693844	26.27585206081056	26.68652958890114	23.376176673594454
90-94	23.698850062993827	26.01509211139786	26.688145225199477	23.597912600408833
95-99	23.685028513104772	26.20507050687334	26.759720162035023	23.350180817986868
100-104	23.95053403794875	25.953336229190565	26.703426200052384	23.392703532808298
105-109	23.749216594342546	26.181518770151506	26.582495964323993	23.48676867118195
110-114	23.854660877867673	26.297523381610493	26.48704532514386	23.360770415377974
115-119	23.971245788717958	26.356531813586866	26.66562292412507	23.00659947357011
120-124	23.945838367356767	26.520507973962577	26.780126821505064	22.7535268371756
125-129	23.914739009814117	26.591866059504397	26.60471326479859	22.888681665882896
130-134	24.103074829661953	26.61929658521781	26.575838728043355	22.70178985707688
135-139	23.867905822678754	26.626221078517997	26.860295379053113	22.645577719750136
140-144	23.885785162157553	26.814752548951766	26.613763649840642	22.685698639050038
145-149	23.708685033623134	27.23431063102654	26.319743244462224	22.737261090888104
150	23.901500783059927	25.97936052952161	26.547454919651702	23.57168376776676
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	0.5
9	2.5
10	2.5
11	1.0
12	7.0
13	10.5
14	15.5
15	23.5
16	40.0
17	83.5
18	153.5
19	245.5
20	407.0
21	623.5
22	956.5
23	1556.0
24	2490.0
25	4007.5
26	6427.5
27	10012.5
28	15069.0
29	21728.0
30	31316.5
31	46078.0
32	67433.0
33	93221.5
34	126254.5
35	180945.5
36	258631.5
37	348796.5
38	458775.5
39	599500.0
40	770677.5
41	956713.5
42	1150135.5
43	1335009.0
44	1482896.5
45	1586450.0
46	1622655.0
47	1593781.0
48	1551561.0
49	1508969.5
50	1406270.5
51	1247623.0
52	1075320.5
53	908286.0
54	764832.0
55	650384.5
56	543426.0
57	445210.5
58	371470.0
59	307540.5
60	236858.0
61	161895.0
62	100196.0
63	60458.5
64	36940.5
65	18104.5
66	9563.5
67	5035.5
68	2410.5
69	1389.5
70	935.5
71	741.5
72	458.5
73	366.5
74	317.5
75	197.5
76	123.0
77	47.5
78	14.0
79	2.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	8.267884279086832E-7
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	8.268781095423147E-7
130-134	0.0022343254708671414
135-139	8.269920068658861E-7
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62-63	1.0
64-65	0.0
66-67	1.0
68-69	3.0
70-71	0.0
72-73	6.0
74-75	7.0
76-77	8.0
78-79	9.0
80-81	11.0
82-83	20.0
84-85	24.0
86-87	31.0
88-89	31.0
90-91	52.0
92-93	51.0
94-95	69.0
96-97	52.0
98-99	68.0
100-101	90.0
102-103	91.0
104-105	100.0
106-107	97.0
108-109	118.0
110-111	125.0
112-113	126.0
114-115	141.0
116-117	193.0
118-119	199.0
120-121	211.0
122-123	293.0
124-125	265.0
126-127	345.0
128-129	400.0
130-131	513.0
132-133	580.0
134-135	874.0
136-137	1404.0
138-139	4400.0
140-141	8685.0
142-143	9319.0
144-145	59265.0
146-147	789105.0
148-149	1002098.0
150-151	2.2310553E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.61082262721069
#Duplication Level	Percentage of total
1	25.395444136462203
2	8.24660123107788
3	5.032822148930084
4	3.5619477188256456
5	2.6824788769221453
6	2.1814615128124406
7	1.803076199096518
8	1.5175169709016603
9	1.2679878154732906
>10	16.428260405233832
>50	5.872662320460847
>100	13.252781160180326
>500	4.604300315976552
>1k	6.397976731947286
>5k	1.1220821484849304
>10k+	0.6326003072143457
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	32860	0.13584106578767108	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.885897804029544E-4	4.1339338340739824E-6	0.0	0.0	0.0022405921380680986	0.0
2	0.001959484637351068	1.653573533629593E-5	0.0	0.0	0.010520861607718287	0.0
3	0.0029516287575288235	2.0669669170369915E-5	0.0	0.0	0.01497724228085004	0.0
4	0.007135169797611694	2.0669669170369915E-5	0.0	0.0	0.02217028715213877	0.0
5	0.007350134356983541	2.0669669170369915E-5	0.0	0.0	0.0270276594071757	0.0
6	0.008569644838035366	2.0669669170369915E-5	0.0	0.0	0.03537820575200514	0.0
7	0.010942522858793833	2.4803603004443896E-5	0.0	0.0	0.04816859703463005	4.1339338340739824E-6
8	0.019053301041246987	2.4803603004443896E-5	0.0	0.0	0.06607266447000447	4.1339338340739824E-6
9	0.019673391116358085	2.4803603004443896E-5	0.0	0.0	0.07905735064283084	4.1339338340739824E-6
10-11	0.020469173379417324	2.4803603004443896E-5	0.0	0.0	0.13166579261525635	4.1339338340739824E-6
12-13	0.021391040624415825	2.8937536838517878E-5	0.0	0.0	0.15735819139402615	4.1339338340739824E-6
14-15	0.024958625523221672	2.8937536838517878E-5	0.0	0.0	0.16917504125872662	4.1339338340739824E-6
16-17	0.029169037133226022	2.8937536838517878E-5	0.0	0.0	0.17715973445924052	4.1339338340739824E-6
18-19	0.037531985279557686	3.1004503755554865E-5	0.0	0.0	0.1848240477876137	4.1339338340739824E-6
20-21	0.04452866829372791	3.307147067259186E-5	0.0	0.0	0.18990258550277359	4.1339338340739824E-6
22-23	0.047414154109911545	3.720540450666584E-5	0.0	4.1339338340739824E-6	0.1951382127036283	4.1339338340739824E-6
24-25	0.05359851912568622	3.9272371423702835E-5	0.0	4.1339338340739824E-6	0.20036763900373186	4.1339338340739824E-6
26-27	0.05880107485586833	4.547327217481381E-5	0.0	4.1339338340739824E-6	0.204454032598714	4.1339338340739824E-6
28-29	0.07147158205730508	5.167417292592478E-5	0.0	4.1339338340739824E-6	0.2080918943726991	4.1339338340739824E-6
30-31	0.0961304973775564	5.3741139842961774E-5	0.0	4.1339338340739824E-6	0.21214108256317457	4.1339338340739824E-6
32-33	0.11692418456294852	5.7875073677035756E-5	0.0	4.1339338340739824E-6	0.2155908503477093	4.1339338340739824E-6
34-35	0.13219907007985188	5.7875073677035756E-5	0.0	4.1339338340739824E-6	0.21904681903299517	4.1339338340739824E-6
36-37	0.1348592565020785	5.7875073677035756E-5	0.0	4.1339338340739824E-6	0.22106211177710622	4.1339338340739824E-6
38-39	0.13709364773939547	5.7875073677035756E-5	0.0	4.1339338340739824E-6	0.2228045648881684	4.1339338340739824E-6
40-41	0.13855499334974064	5.994204059407275E-5	0.0	4.1339338340739824E-6	0.2244643393225491	6.200900751110974E-6
42-43	0.13884436871812583	6.407597442814673E-5	0.0	4.1339338340739824E-6	0.22593395280056242	8.267867668147965E-6
44-45	0.13968562425335987	6.82099082622207E-5	0.0	4.1339338340739824E-6	0.22714312844702905	8.267867668147965E-6
46-47	0.14115317076445616	8.061170976444266E-5	0.0	4.1339338340739824E-6	0.22858380438820383	8.267867668147965E-6
48-49	0.14244915902143834	8.267867668147966E-5	4.1339338340739824E-6	4.1339338340739824E-6	0.22974130586174454	8.267867668147965E-6
50-51	0.14336482536568573	8.681261051555363E-5	4.1339338340739824E-6	4.1339338340739824E-6	0.23094428060746008	8.267867668147965E-6
52-53	0.14417094246333015	9.301351126666461E-5	4.1339338340739824E-6	4.1339338340739824E-6	0.2320955811802497	1.0334834585184957E-5
54-55	0.14460913944974196	9.921441201777559E-5	4.1339338340739824E-6	4.1339338340739824E-6	0.23318280577861114	1.2401801502221948E-5
56-57	0.14537185024212862	9.921441201777559E-5	4.1339338340739824E-6	4.1339338340739824E-6	0.23404266401609852	1.2401801502221948E-5
58-59	0.14552273882707234	1.0334834585184957E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.23482604447765554	1.2401801502221948E-5
60-61	0.14575837305561457	1.0748227968592356E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.2355949561707933	1.2401801502221948E-5
62-63	0.14597127064806936	1.198840811881455E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.23655402882029847	1.2401801502221948E-5
64-65	0.1460022751518249	1.2195104810518249E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.2373539450171918	1.2401801502221948E-5
66-67	0.14608288686158938	1.2815194885629346E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.23796163329080067	1.2401801502221948E-5
68-69	0.1461242261999301	1.2815194885629346E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.23877188432227917	1.2401801502221948E-5
70-71	0.14625031118186937	1.3435284960740444E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.23945398340490137	1.2401801502221948E-5
72-73	0.14631025322246344	1.4882161802666336E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.23994385456423914	1.653573533629593E-5
74-75	0.1463309228916338	1.5915645261184834E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.24049780169800505	1.653573533629593E-5
76-77	0.14635986042847232	1.694912871970333E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.24121297225129984	2.0669669170369915E-5
78-79	0.14639293189914493	1.8189308869925524E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.24220718333839464	2.4803603004443896E-5
80-81	0.14641980246906638	1.922279232844402E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.24295749232927907	3.307147067259186E-5
82-83	0.146454940906656	2.0049579095258817E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.24368299771715907	3.720540450666584E-5
84-85	0.14649628024499678	2.1083062553777312E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.244462244244882	3.720540450666584E-5
86-87	0.1465148829472501	2.149645593718471E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.24522702200418567	3.720540450666584E-5
88-89	0.14651901688108418	2.149645593718471E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.24596286222665087	3.720540450666584E-5
90-91	0.14652521778183528	2.3150029470814302E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.24651887632733382	3.720540450666584E-5
92-93	0.14653555261642046	2.3150029470814302E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.24703561805659308	3.720540450666584E-5
94-95	0.1465934276900975	2.3563422854221703E-4	8.267867668147965E-6	4.1339338340739824E-6	0.2477363198414686	3.720540450666584E-5
96-97	0.14660169555776564	2.3563422854221703E-4	1.0334834585184957E-5	4.1339338340739824E-6	0.2483998162218375	4.133933834073983E-5
98-99	0.14661203039235082	2.3563422854221703E-4	1.2401801502221948E-5	4.1339338340739824E-6	0.2489764999916908	4.340630525777682E-5
100-101	0.14662649916077009	2.3563422854221703E-4	1.2401801502221948E-5	1.2401801502221948E-5	0.2495077104893693	4.960720600888779E-5
102-103	0.14664096792918935	2.3563422854221703E-4	1.4468768419258939E-5	1.2401801502221948E-5	0.2500161843509604	4.960720600888779E-5
104-105	0.14668024030061305	2.3563422854221703E-4	1.653573533629593E-5	1.2401801502221948E-5	0.2505225912456345	4.960720600888779E-5
106-107	0.14670711087053454	2.4183512929332797E-4	1.653573533629593E-5	1.2401801502221948E-5	0.2510207302726404	4.960720600888779E-5
108-109	0.14672364660587084	2.48036030044439E-4	1.860270225333292E-5	1.2401801502221948E-5	0.25147132906055447	4.960720600888779E-5
110-111	0.14677325381187972	2.521699638785129E-4	2.0669669170369915E-5	1.2401801502221948E-5	0.25204181192965663	4.960720600888779E-5
112-113	0.14678565561338194	2.5630389771258693E-4	2.0669669170369915E-5	1.2401801502221948E-5	0.252564754559667	5.3741139842961774E-5
114-115	0.14679185651413304	2.5630389771258693E-4	2.0669669170369915E-5	1.2401801502221948E-5	0.2530339560498344	5.3741139842961774E-5
116-117	0.14679805741488416	2.5630389771258693E-4	2.2736636087406906E-5	1.2401801502221948E-5	0.25357550138209806	5.3741139842961774E-5
118-119	0.14681045921638639	2.5630389771258693E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.25407570737602103	5.580810675999877E-5
120-121	0.14682492798480562	2.5837086462962393E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.254685462616547	5.994204059407275E-5
122-123	0.14684766462089305	2.6250479846369793E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.2551732668089677	6.200900751110974E-5
124-125	0.1468724682238975	2.645717653807349E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.2557871559833277	6.200900751110974E-5
126-127	0.14690140576073601	2.645717653807349E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.2563225004148403	6.200900751110974E-5
128-129	0.1469179414960723	2.645717653807349E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.256923987787698	6.200900751110974E-5
130-131	0.14692414239682342	2.645717653807349E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.2576102208041543	6.407597442814673E-5
132-133	0.14694067813215972	2.645717653807349E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.25825304751535283	6.614294134518372E-5
134-135	0.14694687903291082	2.666387322977719E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.2587759901453632	6.614294134518372E-5
136-137	0.14695514690057898	2.707726661318459E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.2592989327753735	6.614294134518372E-5
138-139	0.14695721386749602	2.7697356688295683E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.2598611477768076	7.027687517925771E-5
>>END_MODULE
