##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.2d.S.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24075142
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	67-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.01712662795509	37.0	35.0	38.0	33.0	39.0
2	35.40015693365381	35.0	34.0	37.0	33.0	39.0
3	35.51742664695394	35.0	34.0	37.0	33.0	39.0
4	35.58850714151551	36.0	35.0	38.0	33.0	39.0
5	35.5065117788298	36.0	35.0	38.0	33.0	39.0
6	35.45106865828662	35.0	35.0	37.0	33.0	39.0
7	35.480486968674995	35.0	34.0	38.0	33.0	39.0
8	35.59285810235304	36.0	35.0	38.0	33.0	39.0
9	35.57702658617756	36.0	35.0	38.0	33.0	39.0
10-14	35.55232424381961	35.8	35.0	37.8	33.0	39.0
15-19	35.530834634329466	36.0	35.0	38.0	33.0	39.0
20-24	35.52097067589466	36.0	35.0	38.0	33.0	39.0
25-29	35.505726670272594	36.0	35.0	38.0	33.0	39.0
30-34	35.489412049989156	36.0	35.0	38.0	33.0	39.0
35-39	35.46287576621563	36.0	34.4	38.0	33.0	39.0
40-44	35.448239333333945	36.0	34.0	38.0	33.0	39.0
45-49	35.419613009966874	36.0	34.0	38.0	33.0	39.0
50-54	35.39961561182069	36.0	34.0	38.0	33.0	39.0
55-59	35.37521614618098	36.0	34.0	38.0	32.2	39.0
60-64	35.33443987163191	36.0	34.0	38.0	32.0	39.0
65-69	35.3085714705836	36.0	34.0	38.0	32.0	39.0
70-74	35.27827852316854	36.0	34.0	38.0	32.0	39.0
75-79	35.22933044904116	36.0	34.0	38.0	32.0	39.0
80-84	35.187868551972045	36.0	34.0	38.0	32.0	39.0
85-89	35.14025634326942	36.0	34.0	38.0	32.0	39.0
90-94	35.08979273648523	36.0	34.0	38.0	32.0	39.0
95-99	35.024295733687794	35.2	34.0	37.8	32.0	39.0
100-104	34.97968074590612	35.2	34.0	38.0	31.0	39.0
105-109	34.92156544320729	35.0	34.0	37.2	31.0	39.0
110-114	34.847453759398476	35.0	34.0	37.0	31.0	39.0
115-119	34.776626849492224	35.0	34.0	37.0	31.0	39.0
120-124	34.70558532517486	35.0	34.0	37.0	30.6	39.0
125-129	34.628286147689735	35.0	34.0	37.0	30.0	39.0
130-134	34.52627283368879	35.0	34.0	37.0	30.0	39.0
135-139	34.47051513691359	35.0	34.0	37.0	30.0	39.0
140-144	34.353496010497636	35.0	34.0	37.0	29.8	39.0
145-149	34.338535843103806	35.0	34.0	37.0	29.4	39.0
150	35.09464899426403	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	121.0
22	1282.0
23	7458.0
24	26735.0
25	67220.0
26	135338.0
27	233237.0
28	346942.0
29	462020.0
30	605081.0
31	799004.0
32	1081268.0
33	1523542.0
34	2300153.0
35	3938993.0
36	1.0273122E7
37	2273618.0
38	4.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.22938415067292	18.823236847367298	15.942622477574586	33.00475652438519
2	23.5835286039019	25.449507213706152	32.94783889540506	18.019125286986885
3	20.07439457677965	29.118075399098377	29.67227358409766	21.13525644002432
4	23.471309120419726	34.010353916084895	21.321066351342807	21.19727061215257
5	25.62136497471126	34.808197600662126	21.600620258023813	17.969817166602798
6	19.330054211102887	38.570397632545635	22.03278385647736	20.06676429987412
7	20.49446271178795	20.340341087084763	37.00997900656204	22.15521719456525
8	21.745815663309482	23.014514306914577	28.048087940665106	27.19158208911083
9	23.62371528275929	23.14727780214131	27.646923120951893	25.582083794147508
10-14	23.10338937980096	28.442680836524247	25.251535380352063	23.202394403322728
15-19	23.201953284429226	27.575001634465956	26.60534504843211	22.617700032672705
20-24	23.040265349213723	27.652830458902383	26.478189827499254	22.82871436438464
25-29	23.16134459352306	27.270742577551566	26.434972636921515	23.13294019200385
30-34	23.016002979338605	26.973555545383697	26.6397058011122	23.370735674165495
35-39	23.35885453967416	26.737007823256036	26.649585701301366	23.254551935768436
40-44	23.518964278748296	26.395668415128505	26.6672099102652	23.418157395857996
45-49	23.427831079874835	26.226674800090482	26.764182740853613	23.581311379181066
50-54	23.614451785995698	26.21959446802017	26.513297408588492	23.65265633739564
55-59	23.636594957570757	26.201636526172926	26.568242048167356	23.593526468088953
60-64	23.295325111685738	26.126591485940143	26.70474300836938	23.87334039400474
65-69	23.502621866242677	26.04204539410967	26.7151554650072	23.740177274640452
70-74	23.6572103857283	26.008950195915386	26.458181269475432	23.875658148880877
75-79	23.532174733822135	26.07179282708403	26.70491771261421	23.69111472647962
80-84	23.84685652579734	26.07071845100919	26.41395286393051	23.66847215926296
85-89	23.528947285697264	26.22061330494388	26.62698513441603	23.623454274942823
90-94	23.55623318341245	25.933880708593026	26.645261193862773	23.864624914131756
95-99	23.56459681818207	26.099655406886768	26.74099913203119	23.594748642899972
100-104	23.828080123252125	25.868389844617855	26.650292963717632	23.653237068412388
105-109	23.644695212553593	26.12136305551961	26.51918529060789	23.714756441318915
110-114	23.716219174426808	26.19632911504927	26.471080978941426	23.6163707315825
115-119	23.8559545756878	26.309902122453476	26.619280803046845	23.21486249881188
120-124	23.79986749282584	26.491745244369163	26.721935895801675	22.986451367003326
125-129	23.820420344798237	26.519993232131288	26.549914423349296	23.109671999721176
130-134	23.98789161789009	26.54468473873088	26.53334119384634	22.934082449532696
135-139	23.795834367707826	26.58086795456359	26.800242872072605	22.823054805655975
140-144	23.825944363680478	26.719390960401558	26.586363424612898	22.868301251305066
145-149	23.58889658438805	26.974615056045824	26.447549429469692	22.98893893009643
150	23.81787669535005	25.801716262468084	26.69619046799338	23.68421657418849
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	1.5
11	3.0
12	5.5
13	11.0
14	19.0
15	26.0
16	46.0
17	89.0
18	164.0
19	261.0
20	405.5
21	631.5
22	961.0
23	1536.0
24	2486.5
25	4046.5
26	6427.0
27	10063.5
28	15376.5
29	21843.0
30	30958.0
31	44963.5
32	65543.5
33	90441.0
34	121457.0
35	174877.0
36	249602.0
37	335548.0
38	442396.0
39	581684.0
40	754616.5
41	938877.5
42	1128052.0
43	1311671.5
44	1458862.5
45	1566502.0
46	1604252.5
47	1576163.5
48	1543867.0
49	1512189.0
50	1413806.5
51	1257562.0
52	1089442.5
53	921969.0
54	773989.0
55	661156.5
56	556773.5
57	456821.5
58	381320.0
59	315860.0
60	243692.0
61	165107.5
62	101397.5
63	62126.0
64	38222.0
65	18227.5
66	9559.0
67	5010.0
68	2432.0
69	1280.5
70	731.0
71	574.5
72	366.0
73	277.5
74	246.0
75	152.5
76	100.5
77	37.5
78	16.0
79	4.0
80	2.0
81	1.5
82	1.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	8.30732379480877E-7
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	8.307324001843662E-7
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	8.307972278689202E-7
125-129	4.154105344455923E-6
130-134	0.050069063269492384
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
66-67	1.0
68-69	1.0
70-71	2.0
72-73	4.0
74-75	6.0
76-77	9.0
78-79	11.0
80-81	15.0
82-83	19.0
84-85	16.0
86-87	23.0
88-89	30.0
90-91	40.0
92-93	44.0
94-95	61.0
96-97	63.0
98-99	86.0
100-101	95.0
102-103	91.0
104-105	107.0
106-107	95.0
108-109	107.0
110-111	136.0
112-113	122.0
114-115	147.0
116-117	146.0
118-119	175.0
120-121	210.0
122-123	260.0
124-125	281.0
126-127	326.0
128-129	366.0
130-131	463.0
132-133	584.0
134-135	899.0
136-137	1387.0
138-139	4811.0
140-141	8903.0
142-143	9304.0
144-145	59218.0
146-147	784571.0
148-149	1010546.0
150-151	2.2191361E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.51153403918746
#Duplication Level	Percentage of total
1	24.383019221972148
2	8.033772441062464
3	5.057075339259389
4	3.5532374263621556
5	2.6849470225691805
6	2.137117553625128
7	1.8233992019440866
8	1.4898870366679065
9	1.3399025681339025
>10	16.55696901547065
>50	6.068996857561672
>100	13.620411535378684
>500	4.823564380137192
>1k	6.644545014973777
>5k	0.9075337046890248
>10k+	0.8756216801925868
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	28660	0.11904394997960968	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.2845140435724117E-4	0.0	0.0	0.0	0.0021059065819840233	0.0
2	0.0012211765978368892	1.661464758961754E-5	0.0	0.0	0.009769412782695113	0.0
3	0.0017653063063968635	1.661464758961754E-5	0.0	0.0	0.014213831012917805	0.0
4	0.004564874425247419	1.661464758961754E-5	0.0	0.0	0.02063123864440758	0.0
5	0.004735174563040999	1.661464758961754E-5	0.0	0.0	0.025237649688629044	0.0
6	0.005632365532880346	1.661464758961754E-5	0.0	0.0	0.03256886293754778	0.0
7	0.007588740286557811	2.9075633281830694E-5	0.0	0.0	0.04383774766520588	0.0
8	0.014267828617584062	2.9075633281830694E-5	0.0	0.0	0.05946797738513858	0.0
9	0.014915799873579147	2.9075633281830694E-5	0.0	0.0	0.07111484534546048	0.0
10-11	0.015609461410445679	3.322929517923508E-5	2.0768309487021926E-6	0.0	0.11615715496091361	0.0
12-13	0.016344659566286255	3.7382957076639467E-5	4.153661897404385E-6	0.0	0.1380988739339523	0.0
14-15	0.019071538601932232	3.7382957076639467E-5	4.153661897404385E-6	0.0	0.14821719431602937	0.0
16-17	0.022267781431984907	4.153661897404385E-5	4.153661897404385E-6	0.0	0.15568132474566504	0.0
18-19	0.029758910663953718	4.153661897404385E-5	4.153661897404385E-6	0.0	0.16357120551978468	0.0
20-21	0.036201240266827917	4.153661897404385E-5	4.153661897404385E-6	0.0	0.16848083388251667	0.0
22-23	0.03913164873544671	4.5690280871448235E-5	4.153661897404385E-6	0.0	0.17358568435442664	0.0
24-25	0.04592080910675418	4.5690280871448235E-5	4.153661897404385E-6	0.0	0.17880060686661786	0.0
26-27	0.05164455520137742	4.5690280871448235E-5	4.153661897404385E-6	0.0	0.18295634559497095	0.0
28-29	0.06489473665409741	4.5690280871448235E-5	4.153661897404385E-6	0.0	0.187091316013837	0.0
30-31	0.09156955335922837	4.984394276885262E-5	4.153661897404385E-6	0.0	0.19142358537282977	4.153661897404385E-6
32-33	0.11159435736661491	5.3997604666257004E-5	4.153661897404385E-6	0.0	0.1945658305982162	4.153661897404385E-6
34-35	0.12576042126771259	5.3997604666257004E-5	4.153661897404385E-6	0.0	0.19792614307321635	4.153661897404385E-6
36-37	0.12776663996415888	5.3997604666257004E-5	4.153661897404385E-6	0.0	0.20000712768381596	4.153661897404385E-6
38-39	0.12961709633945254	5.8151266563661396E-5	4.153661897404385E-6	0.0	0.20157513505008612	4.153661897404385E-6
40-41	0.1309130388514427	6.230492846106578E-5	8.30732379480877E-6	0.0	0.20320544734481732	4.153661897404385E-6
42-43	0.13118302687477398	6.438175940976797E-5	8.30732379480877E-6	0.0	0.2047879925277284	4.153661897404385E-6
44-45	0.13198883728287045	7.476591415327893E-5	8.30732379480877E-6	2.0768309487021926E-6	0.20607355088497503	4.153661897404385E-6
46-47	0.13346338725644902	7.891957605068332E-5	8.30732379480877E-6	4.153661897404385E-6	0.2073674165660165	4.153661897404385E-6
48-49	0.1346845638542859	8.099640699938551E-5	8.30732379480877E-6	4.153661897404385E-6	0.20860936147334042	4.153661897404385E-6
50-51	0.13566898172397074	8.30732379480877E-5	8.30732379480877E-6	4.153661897404385E-6	0.2098160002545364	4.153661897404385E-6
52-53	0.1365973251580406	8.30732379480877E-5	8.30732379480877E-6	4.153661897404385E-6	0.21100187072624535	4.153661897404385E-6
54-55	0.13707914993813952	9.345739269159866E-5	8.30732379480877E-6	4.153661897404385E-6	0.21206520817198088	4.153661897404385E-6
56-57	0.13774373584172422	9.761105458900305E-5	8.30732379480877E-6	6.230492846106578E-6	0.21308285533684496	4.153661897404385E-6
58-59	0.13787042252959503	9.968788553770524E-5	8.30732379480877E-6	8.30732379480877E-6	0.2140070451090174	4.153661897404385E-6
60-61	0.1381881776647465	1.0384154743510962E-4	8.30732379480877E-6	8.30732379480877E-6	0.21471109080062745	4.153661897404385E-6
62-63	0.13845816568807778	1.0799520933251401E-4	8.30732379480877E-6	8.30732379480877E-6	0.2156145122633129	4.153661897404385E-6
64-65	0.13847478033566737	1.1214887122991839E-4	8.30732379480877E-6	8.30732379480877E-6	0.216534548373588	4.153661897404385E-6
66-67	0.13854954624982066	1.2045619502472716E-4	8.30732379480877E-6	8.30732379480877E-6	0.2172074416009675	6.230492846106578E-6
68-69	0.1386014670235382	1.3291718071694033E-4	8.30732379480877E-6	8.30732379480877E-6	0.2179509470806029	8.30732379480877E-6
70-71	0.13870946223287073	1.349940116656425E-4	8.30732379480877E-6	8.30732379480877E-6	0.21867368425075123	8.30732379480877E-6
72-73	0.13876345983753702	1.370708426143447E-4	8.30732379480877E-6	8.30732379480877E-6	0.21916173952369628	1.0384154743510963E-5
74-75	0.138784228147024	1.4537816640915348E-4	1.661464758961754E-5	8.30732379480877E-6	0.2197660973297686	1.2460985692213155E-5
76-77	0.13880915011840844	1.5160865925526005E-4	1.661464758961754E-5	1.2460985692213155E-5	0.22057398456881377	1.2460985692213155E-5
78-79	0.1388797623706643	1.5783915210136664E-4	1.661464758961754E-5	1.2460985692213155E-5	0.2216663976478311	1.2460985692213155E-5
80-81	0.13897737342525332	1.6199281399877102E-4	2.0768309487021926E-5	1.2460985692213155E-5	0.2224161336203126	1.4537816640915349E-5
82-83	0.13900437222758644	1.6199281399877102E-4	2.0768309487021926E-5	1.2460985692213155E-5	0.22321155987366553	1.661464758961754E-5
84-85	0.13906044666320141	1.6199281399877102E-4	2.0768309487021926E-5	1.2460985692213155E-5	0.2240090629579672	1.661464758961754E-5
86-87	0.13909990645122675	1.6199281399877102E-4	2.2845140435724118E-5	1.2460985692213155E-5	0.2247857977327818	1.661464758961754E-5
88-89	0.1391372894083034	1.661464758961754E-4	2.492197138442631E-5	1.2460985692213155E-5	0.2255542251838016	1.661464758961754E-5
90-91	0.13917259553443131	1.661464758961754E-4	2.492197138442631E-5	1.2460985692213155E-5	0.22612120003279731	2.0768309487021926E-5
92-93	0.13919336384391834	1.661464758961754E-4	2.9075633281830694E-5	1.2460985692213155E-5	0.22661963946048586	2.0768309487021926E-5
94-95	0.13924113095573848	1.72376968742282E-4	2.9075633281830694E-5	1.2460985692213155E-5	0.22722815092845558	2.0768309487021926E-5
96-97	0.1392432077866872	1.7445379969098417E-4	2.9075633281830694E-5	1.2460985692213155E-5	0.22780343310124607	2.492197138442631E-5
98-99	0.1392577456033281	1.8276112348579294E-4	2.9075633281830694E-5	1.2460985692213155E-5	0.2282790273884989	2.492197138442631E-5
100-101	0.13927851391281515	1.8276112348579294E-4	2.9075633281830694E-5	1.2460985692213155E-5	0.2287650058304952	2.492197138442631E-5
102-103	0.13929512856040477	1.8276112348579294E-4	3.5306126127937274E-5	1.2460985692213155E-5	0.22923644645585062	2.492197138442631E-5
104-105	0.13930966637704567	1.8276112348579294E-4	3.7382957076639467E-5	1.2460985692213155E-5	0.22982626644528203	2.492197138442631E-5
106-107	0.1393345883484301	1.8691478538319733E-4	3.7382957076639467E-5	1.2460985692213155E-5	0.23032470587297055	2.492197138442631E-5
108-109	0.1393574334888658	1.931452782293039E-4	4.5690280871448235E-5	1.2460985692213155E-5	0.23086260508868442	2.492197138442631E-5
110-111	0.13939273961499377	1.972989401267083E-4	4.5690280871448235E-5	1.4537816640915349E-5	0.2314316567686288	2.492197138442631E-5
112-113	0.13940520060068598	2.0352943297281486E-4	4.5690280871448235E-5	1.661464758961754E-5	0.23200278527952192	2.492197138442631E-5
114-115	0.1394197384173269	2.0352943297281486E-4	4.5690280871448235E-5	1.661464758961754E-5	0.2324700722429799	2.6998802333128502E-5
116-117	0.1394363530649165	2.0768309487021925E-4	4.5690280871448235E-5	1.661464758961754E-5	0.23303497026102693	3.322929517923508E-5
118-119	0.13944673721966	2.1183675676762363E-4	4.984394276885262E-5	1.661464758961754E-5	0.23353133285776673	3.5306126127937274E-5
120-121	0.1394550445434548	2.1599041866502802E-4	4.984394276885262E-5	1.661464758961754E-5	0.2341626894661722	3.7382957076639467E-5
122-123	0.1394695823600957	2.1599041866502802E-4	4.984394276885262E-5	1.661464758961754E-5	0.23466735938670683	3.7382957076639467E-5
124-125	0.13948412017673664	2.201440805624324E-4	4.984394276885262E-5	1.661464758961754E-5	0.2353174074736506	3.7382957076639467E-5
126-127	0.13949658116242886	2.2637457340853896E-4	5.3997604666257004E-5	1.661464758961754E-5	0.2358553066893645	4.5690280871448235E-5
128-129	0.13951319581001848	2.3468189720334773E-4	5.3997604666257004E-5	1.661464758961754E-5	0.236430588862155	4.984394276885262E-5
130-131	0.1395422714433003	2.471428828955609E-4	5.3997604666257004E-5	1.661464758961754E-5	0.2371720175108417	5.815126656366139E-5
132-133	0.13955057876709512	2.492197138442631E-4	5.3997604666257004E-5	1.661464758961754E-5	0.23777637531691403	6.230492846106578E-5
134-135	0.13956303975278733	2.492197138442631E-4	5.3997604666257004E-5	1.661464758961754E-5	0.2382395086184746	6.230492846106578E-5
136-137	0.13957134707658214	2.533733757416675E-4	5.3997604666257004E-5	1.661464758961754E-5	0.23875040903185535	6.230492846106578E-5
138-139	0.13957134707658214	2.533733757416675E-4	5.3997604666257004E-5	1.661464758961754E-5	0.2392945387404153	6.645859035847016E-5
>>END_MODULE
