##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.2d.S.3.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24094736
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	66-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.41289479162585	36.0	34.0	38.0	31.0	39.0
2	34.735854462153064	35.0	34.0	38.0	31.0	39.0
3	34.896790236672445	35.0	34.0	38.0	31.0	39.0
4	34.85341225568938	36.0	34.0	38.0	31.0	39.0
5	34.72619085762135	35.0	34.0	37.0	31.0	39.0
6	34.726014221529546	35.0	34.0	37.0	31.0	39.0
7	34.734614813791694	35.0	34.0	37.0	32.0	39.0
8	34.88707994144447	35.0	34.0	37.0	31.0	39.0
9	34.86625887911783	35.0	34.0	37.0	31.0	39.0
10-14	34.83541951237814	35.0	34.0	37.4	31.0	39.0
15-19	34.83798297686267	35.0	34.0	37.0	31.0	39.0
20-24	34.84109288435449	35.0	34.0	37.0	31.0	39.0
25-29	34.83959814293047	35.0	34.0	37.0	31.0	39.0
30-34	34.81800661356074	35.0	34.0	37.0	31.0	39.0
35-39	34.81455774406493	35.0	34.0	37.0	31.0	39.0
40-44	34.745996038304796	35.0	34.0	37.0	31.0	39.0
45-49	34.64525836680676	35.0	34.0	37.0	31.0	39.0
50-54	34.60966810343969	35.0	34.0	37.0	30.2	39.0
55-59	34.60159188297394	35.0	34.0	37.0	30.0	39.0
60-64	34.57049757258183	35.0	34.0	37.0	30.0	39.0
65-69	34.55320211548168	35.0	34.0	37.0	30.0	39.0
70-74	34.526753952813166	35.0	34.0	37.0	30.0	39.0
75-79	34.50655930135012	35.0	34.0	37.0	30.0	39.0
80-84	34.47703646254315	35.0	34.0	37.0	30.0	39.0
85-89	34.46086509928911	35.0	34.0	37.0	30.0	39.0
90-94	34.43179125688494	35.0	34.0	37.0	30.0	39.0
95-99	34.395014642057745	35.0	34.0	37.0	30.0	39.0
100-104	34.358903961048	35.0	34.0	37.0	30.0	39.0
105-109	34.30285724399911	35.0	34.0	37.0	30.0	39.0
110-114	34.27468353845889	35.0	34.0	37.0	30.0	39.0
115-119	34.22425879965887	35.0	34.0	37.0	29.0	39.0
120-124	34.18748238981575	35.0	34.0	37.0	29.0	39.0
125-129	34.133806917263925	35.0	34.0	37.0	29.0	39.0
130-134	34.087957778434784	35.0	34.0	37.0	29.0	39.0
135-139	34.037686242111015	35.0	34.0	37.0	29.0	39.0
140-144	34.0125026005466	35.0	34.0	37.0	29.0	39.0
145-149	34.02202540355554	35.0	34.0	37.0	29.0	39.0
150	34.95790965412853	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	19.0
22	280.0
23	3437.0
24	22654.0
25	89293.0
26	223245.0
27	402950.0
28	586539.0
29	763069.0
30	943718.0
31	1176209.0
32	1490670.0
33	1943721.0
34	2668655.0
35	4138351.0
36	8181192.0
37	1460726.0
38	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.63009364369047	10.650932220216067	15.239610842799856	38.47936329329361
2	22.677119093284116	12.846767246495618	36.53847909738792	27.937634562832347
3	21.794190136716704	17.544079687184514	27.856224902431926	32.80550527366686
4	26.985735714236505	25.320109524401236	21.74909924256441	25.94505551879785
5	26.632792595493488	30.75642011706127	23.867748352627455	18.743038934817786
6	19.711529283017903	36.14121868047749	25.51261861729352	18.634633419211085
7	16.813936314850437	23.752502159085584	39.99861629269286	19.43494523337112
8	19.40472342566727	23.645350291740314	31.061669288818344	25.88825699377407
9	20.191432021503473	22.738471901410744	32.062591319299685	25.007504757786098
10-14	21.208955873184337	28.439393702575817	26.44850075260133	23.90314967163852
15-19	21.443325491599172	27.480920427946586	27.11990449140985	23.95584958904439
20-24	21.38845684818552	27.45073824300941	27.04656213366936	24.11424277513571
25-29	21.305318230872448	27.763827886185332	26.650205641487673	24.280648241454546
30-34	21.35587016525974	27.541886351372547	26.854838329454612	24.2474051539131
35-39	21.59731730383155	27.321108350242028	26.777644218517793	24.303930127408627
40-44	21.78044850875619	27.382209396931838	26.586689334765897	24.250652759546078
45-49	22.041579636833035	27.329621246501603	26.53899567533946	24.089803441325905
50-54	21.99988140086496	27.559773656937423	26.429979105535082	24.01036583666253
55-59	21.925333840887497	27.47997075033751	26.548175015045878	24.04652039372911
60-64	22.195605832992616	27.33644643571127	26.261089471616184	24.20685825967993
65-69	22.027012595207623	27.463801475915634	26.466291505198637	24.042894423678106
70-74	22.390037584849626	27.212162577764033	26.379856007403202	24.017943829983142
75-79	22.221139271671376	27.146024020236116	26.36867857325144	24.264158134841075
80-84	22.474824919776143	27.07477740215436	26.439206176650025	24.011191501419475
85-89	22.328220382017534	27.090930145805093	26.431403676664317	24.149445795513053
90-94	22.345450938602863	26.833046558893063	26.4066264090917	24.414876093412374
95-99	22.538011929975028	26.894720469293464	26.62241518722608	23.944852413505433
100-104	22.516057535487743	26.70249951891288	26.639779521112235	24.141663424487145
105-109	22.589090357078607	26.71565297401486	26.519557833801592	24.175698835104935
110-114	22.523756785152298	26.763968186820406	26.5106008833615	24.201674144665798
115-119	22.635061667235412	27.007656762926064	26.37700831169793	23.980273258140596
120-124	22.587379774415815	26.82458876288957	26.414858061109165	24.173173401585455
125-129	22.951780892592698	26.78624718330936	26.313361417326142	23.948610506771804
130-134	22.633283978727615	27.04116082470956	26.22067727232153	24.104877924241297
135-139	22.785931361362366	26.802864245081025	26.253196467129214	24.1580079264274
140-144	22.746514781858128	26.644225491480018	26.54795055276493	24.061309173896927
145-149	22.17160996759075	26.671840175230955	26.958834088328764	24.19771576884953
150	21.977944925999555	25.297252034344815	27.60201268930878	25.12279035034685
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.0
10	0.5
11	2.5
12	8.0
13	14.5
14	20.0
15	36.5
16	74.5
17	182.5
18	433.0
19	788.5
20	1258.0
21	2053.5
22	3540.5
23	6035.0
24	9867.0
25	15047.5
26	21275.5
27	31010.5
28	48223.5
29	71414.0
30	97500.0
31	123312.0
32	149310.0
33	183087.0
34	220994.0
35	268378.0
36	338799.5
37	413821.5
38	485932.0
39	592261.5
40	739302.0
41	897410.0
42	1056795.0
43	1212189.0
44	1347186.5
45	1447042.0
46	1527741.0
47	1560082.0
48	1512410.0
49	1459229.0
50	1373720.0
51	1225372.0
52	1053470.0
53	899690.0
54	756633.5
55	628460.0
56	543693.5
57	456449.5
58	375702.0
59	307203.5
60	238445.5
61	165356.0
62	95146.5
63	53481.0
64	33847.5
65	20459.0
66	12786.0
67	5778.0
68	2052.0
69	956.0
70	782.5
71	558.0
72	302.5
73	141.0
74	83.5
75	54.5
76	28.5
77	19.0
78	12.5
79	3.5
80	2.5
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.8261250092136308E-4
3	8.715596634883238E-5
4	5.8103977565888247E-5
5	1.6601136447396644E-5
6	8.300568223698321E-5
7	1.8676278503321225E-4
8	8.300568223698321E-5
9	8.715596634883238E-5
10-14	1.6684142129633626E-4
15-19	2.7225863773730496E-4
20-24	1.7099170540818543E-4
25-29	3.502839790400692E-4
30-34	8.499781861067081E-4
35-39	9.529052320805674E-4
40-44	0.0010740935281465629
45-49	9.155526750739249E-4
50-54	5.19615570803515E-4
55-59	4.108781270730669E-4
60-64	2.3988642166488148E-4
65-69	3.278724530006667E-4
70-74	3.2953257489266906E-4
75-79	2.498471906360128E-4
80-84	2.7142892337310816E-4
85-89	3.5692531946123434E-4
90-94	3.619063156196433E-4
95-99	3.062930115489233E-4
100-104	4.631762396963934E-4
105-109	2.6230174428999817E-4
110-114	2.3740106528498584E-4
115-119	2.4404362557053916E-4
120-124	2.2080386635870891E-4
125-129	1.34476786683491E-4
130-134	1.585538718075178E-4
135-139	1.6188453120082757E-4
140-144	1.5613384819964675E-4
145-149	1.4224701083359161E-4
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
66-67	1.0
68-69	0.0
70-71	0.0
72-73	1.0
74-75	3.0
76-77	4.0
78-79	8.0
80-81	15.0
82-83	11.0
84-85	14.0
86-87	4.0
88-89	16.0
90-91	27.0
92-93	22.0
94-95	24.0
96-97	25.0
98-99	19.0
100-101	48.0
102-103	35.0
104-105	45.0
106-107	51.0
108-109	57.0
110-111	57.0
112-113	64.0
114-115	84.0
116-117	94.0
118-119	85.0
120-121	141.0
122-123	151.0
124-125	152.0
126-127	186.0
128-129	241.0
130-131	274.0
132-133	358.0
134-135	573.0
136-137	1016.0
138-139	2326.0
140-141	5937.0
142-143	9209.0
144-145	73153.0
146-147	780844.0
148-149	1455843.0
150-151	2.1763518E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.519983702118985
#Duplication Level	Percentage of total
1	32.26180572362186
2	8.585488208445387
3	4.95080911866147
4	3.6341005867002645
5	2.6274297587060507
6	2.0751472785145038
7	1.6835393090070534
8	1.473054097252304
9	1.2281763193876634
>10	15.670821996113832
>50	5.213560543621896
>100	11.162196821075531
>500	4.0848306077942365
>1k	4.777336853245749
>5k	0.4635076297212878
>10k+	0.10819514813110162
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	8.300568223698322E-6	2.9051988782944127E-5	0.0	1.2450852335547482E-5	3.237221607242345E-4	1.2450852335547482E-5
2	2.9051988782944127E-5	7.885539812513406E-5	0.0	2.4901704671094965E-5	0.002407164784872513	3.320227289479329E-5
3	3.735255700664245E-5	8.715596634883238E-5	0.0	2.4901704671094965E-5	0.004673219909942155	4.980340934218993E-5
4	8.715596634883238E-5	9.54565345725307E-5	0.0	2.9051988782944127E-5	0.006847968784551115	5.395369345403909E-5
5	1.0790738690807818E-4	9.54565345725307E-5	0.0	2.9051988782944127E-5	0.007881389528401556	5.810397756588825E-5
6	1.5356051213841895E-4	9.54565345725307E-5	0.0	2.9051988782944127E-5	0.00944604663856869	5.810397756588825E-5
7	2.116644897043072E-4	1.162079551317765E-4	0.0	3.320227289479329E-5	0.01092769806649884	6.225426167773741E-5
8	3.3202272894793285E-4	1.2035823924362566E-4	0.0	3.320227289479329E-5	0.014571647516702403	6.640454578958658E-5
9	4.108781270730669E-4	1.2035823924362566E-4	0.0	3.320227289479329E-5	0.017443644122102024	7.055482990143573E-5
10-11	4.897335251982009E-4	1.4110965980287146E-4	0.0	3.5277414950717864E-5	0.02955209801842195	8.300568223698322E-5
12-13	5.250109401489188E-4	1.4110965980287146E-4	0.0	3.735255700664245E-5	0.037201071636559946	9.54565345725307E-5
14-15	5.519877868759384E-4	1.5148537008249436E-4	0.0	3.735255700664245E-5	0.042747926352046356	1.0375710279622902E-4
16-17	5.976409121062792E-4	1.5356051213841895E-4	0.0	3.735255700664245E-5	0.046331696682628105	1.058322448521536E-4
18-19	6.910223046228852E-4	1.597859383061927E-4	0.0	3.735255700664245E-5	0.04936555436838984	1.0790738690807818E-4
20-21	0.0010583224485215359	2.0543906353653348E-4	0.0	3.942769906256703E-5	0.051843273983163796	1.0790738690807818E-4
22-23	0.0014173220241964885	2.1581477381615635E-4	0.0	4.357798317441619E-5	0.054269115046539626	1.0790738690807818E-4
24-25	0.0015335299793282648	2.2411534203985468E-4	0.0	4.565312523034077E-5	0.056802863496823536	1.0790738690807818E-4
26-27	0.0016082350933415497	2.2411534203985468E-4	0.0	4.772826728626535E-5	0.05901704007049507	1.2035823924362566E-4
28-29	0.0017783967419273655	2.32415910263553E-4	0.0	5.3953693454039095E-5	0.06174377673197996	1.28658807467324E-4
30-31	0.0020191132204146166	2.32415910263553E-4	0.0	7.055482990143573E-5	0.06460332248504404	1.390345177469469E-4
32-33	0.0021104194708752985	2.3449105231947758E-4	0.0	7.47051140132849E-5	0.067108018946545	1.4733508597064522E-4
34-35	0.0021955002951682063	2.3656619437540215E-4	0.0	9.54565345725307E-5	0.06972477307906591	1.5563565419434355E-4
36-37	0.002234927994230773	2.3656619437540215E-4	0.0	1.1205767101992734E-4	0.07212363729571472	1.5771079625026812E-4
38-39	0.0022411534203985468	2.3656619437540215E-4	0.0	1.2450852335547482E-4	0.0743772415684488	1.597859383061927E-4
40-41	0.0022577545568459433	2.3656619437540215E-4	0.0	1.2450852335547482E-4	0.07653953959072221	1.6186108036211726E-4
42-43	0.0022702054091814907	2.3656619437540215E-4	0.0	1.3280909157917315E-4	0.0787495658802819	1.6601136447396642E-4
44-45	0.0022868065456288873	2.4071647848725132E-4	0.0	1.3280909157917315E-4	0.08130614089318099	1.701616485858156E-4
46-47	0.0023117082502999826	2.4071647848725132E-4	0.0	1.3280909157917315E-4	0.0850642231564604	1.7223679064174016E-4
48-49	0.0023158585344118317	2.448667625991005E-4	0.0	1.3695937569102232E-4	0.08735102970208929	1.8261250092136306E-4
50-51	0.002334534812915153	2.4901704671094965E-4	0.0	1.4525994391472062E-4	0.08972291707201108	1.8676278503321222E-4
52-53	0.002369812227865871	2.4901704671094965E-4	0.0	1.4525994391472062E-4	0.09178768341765603	1.9713849531283515E-4
54-55	0.002392638790481041	2.4901704671094965E-4	0.0	1.4525994391472062E-4	0.0934809993352905	2.0543906353653348E-4
56-57	0.0024901704671094964	2.5316733082279884E-4	0.0	1.494102280265698E-4	0.09547106056692217	2.2204019998393009E-4
58-59	0.002537898734395762	2.57317614934648E-4	0.0	1.5563565419434355E-4	0.09766448572003444	2.2826562615170385E-4
60-61	0.002679008394198633	2.5939275699057255E-4	0.0	1.5771079625026812E-4	0.09977698033296567	2.3241591026355299E-4
62-63	0.002735037229708597	2.614678990464971E-4	0.0	1.5771079625026812E-4	0.10187287380944951	2.3656619437540215E-4
64-65	0.0027557886502678425	2.614678990464971E-4	0.0	1.6186108036211726E-4	0.10460376075504624	2.3864133643132675E-4
66-67	0.002788990923162636	2.780690354938938E-4	0.0	1.6601136447396642E-4	0.10701300068197468	2.4071647848725132E-4
68-69	0.0028242683381133537	2.9051988782944125E-4	0.0	1.6601136447396642E-4	0.10883290026502054	2.4071647848725132E-4
70-71	0.002859545753064072	2.988204560531396E-4	0.0	1.701616485858156E-4	0.11073165524619152	2.448667625991005E-4
72-73	0.002878222031567393	2.988204560531396E-4	0.0	1.7638707475358935E-4	0.1122216072423454	2.4901704671094965E-4
74-75	0.0029051988782944124	2.988204560531396E-4	0.0	1.9506335325691055E-4	0.11379871520484806	2.4901704671094965E-4
76-77	0.002932175725021432	3.029707401649887E-4	0.0	2.0543906353653345E-4	0.11516000839353459	2.4901704671094965E-4
78-79	0.0029778288502517727	3.029707401649887E-4	0.0	2.1581477381615635E-4	0.11645489703643153	2.4901704671094965E-4
80-81	0.0030151814072584154	3.112713083886871E-4	0.0	2.1581477381615635E-4	0.11788259477090765	2.5316733082279884E-4
82-83	0.00304008311192951	3.237221607242345E-4	0.0	2.1788991587208095E-4	0.11943065074462739	2.614678990464971E-4
84-85	0.003075360526880228	3.3202272894793285E-4	0.0	2.2204019998393009E-4	0.12099530785479451	2.635430411024217E-4
86-87	0.0031085627997750214	3.3617301305978204E-4	0.0	2.2411534203985468E-4	0.12237527732198436	2.656181831583463E-4
88-89	0.003114788225942795	3.403232971716312E-4	0.0	2.2411534203985468E-4	0.12384655303963488	2.656181831583463E-4
90-91	0.0031313893623901917	3.4447358128348037E-4	0.0	2.2411534203985468E-4	0.1259569725105102	2.656181831583463E-4
92-93	0.0031625164932290607	3.4447358128348037E-4	0.0	2.2411534203985468E-4	0.12783705121317784	2.656181831583463E-4
94-95	0.0032226956128508736	3.4654872333940494E-4	0.0	2.2411534203985468E-4	0.12922947153270326	2.656181831583463E-4
96-97	0.0032994758689200827	3.5484929156310327E-4	0.0	2.2411534203985468E-4	0.13061151614194902	2.656181831583463E-4
98-99	0.0033721058408774434	3.693752859545753E-4	4.150284111849161E-6	2.2619048409577928E-4	0.13172586742598053	2.676933252142709E-4
100-101	0.003492464080121069	3.7352557006642444E-4	4.150284111849161E-6	2.2826562615170385E-4	0.13291284868196937	2.6976846727019545E-4
102-103	0.0035235912109599374	3.7560071212234907E-4	4.150284111849161E-6	2.3034076820762842E-4	0.13393381857348427	2.6976846727019545E-4
104-105	0.0035484929156310322	3.7767585417827364E-4	4.150284111849161E-6	2.3864133643132675E-4	0.13500459187434136	2.739187513820446E-4
106-107	0.0036231980296443176	3.7767585417827364E-4	4.150284111849161E-6	2.448667625991005E-4	0.1360712148910866	2.739187513820446E-4
108-109	0.0036916777174898284	3.818261382901228E-4	4.150284111849161E-6	2.448667625991005E-4	0.13701125424242042	2.739187513820446E-4
110-111	0.0037373308427201693	3.818261382901228E-4	4.150284111849161E-6	2.448667625991005E-4	0.13795336873581016	2.780690354938938E-4
112-113	0.0038348625193486245	3.818261382901228E-4	4.150284111849161E-6	2.4901704671094965E-4	0.13905111888339428	2.780690354938938E-4
114-115	0.0038742902184111918	3.8390128034604734E-4	4.150284111849161E-6	2.4901704671094965E-4	0.14016131988331393	2.863696037175921E-4
116-117	0.00395522075859225	3.9220184856974573E-4	4.150284111849161E-6	2.531673308227988E-4	0.14093742301222972	2.863696037175921E-4
118-119	0.004015399878214063	4.0880298501714234E-4	4.150284111849161E-6	2.57317614934648E-4	0.14175710412431994	2.863696037175921E-4
120-121	0.004162734964184708	4.1295326912899153E-4	4.150284111849161E-6	2.656181831583463E-4	0.14274694688499595	2.863696037175921E-4
122-123	0.004405526584727884	4.150284111849161E-4	4.150284111849161E-6	2.7184360932612E-4	0.14371811336716867	2.863696037175921E-4
124-125	0.004530035108083358	4.150284111849161E-4	4.150284111849161E-6	2.780690354938938E-4	0.14462910072971955	2.863696037175921E-4
126-127	0.004581913659481474	4.150284111849161E-4	4.150284111849161E-6	2.780690354938938E-4	0.14556914008105337	2.9051988782944125E-4
128-129	0.0046649193417184565	4.1710355324084067E-4	4.150284111849161E-6	2.780690354938938E-4	0.14633486749968955	2.9259502988536587E-4
130-131	0.004810179285633177	4.2747926352046357E-4	4.150284111849161E-6	2.822193196057429E-4	0.1469698609688025	2.988204560531396E-4
132-133	0.0048848843996464626	4.2955440557638813E-4	4.150284111849161E-6	2.842944616616675E-4	0.14765050756314574	3.071210242768379E-4
134-135	0.004990716644498615	4.316295476323127E-4	4.150284111849161E-6	2.884447457735167E-4	0.14839340841916676	3.071210242768379E-4
136-137	0.005121450594021864	4.357798317441619E-4	4.150284111849161E-6	2.9051988782944125E-4	0.14919648839480956	3.091961663327625E-4
138-139	0.005129751162245563	4.3993011585601103E-4	4.150284111849161E-6	2.9051988782944125E-4	0.15003069550129122	3.1127130838868705E-4
>>END_MODULE
