##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.4d.R.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23543034
Total Bases	3.5 Gbp
Sequences flagged as poor quality	0
Sequence length	62-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.23946777632823	37.0	35.0	38.0	34.0	39.0
2	35.58890685032354	35.0	35.0	38.0	33.0	39.0
3	35.68987705662745	36.0	35.0	38.0	34.0	39.0
4	35.7948476819088	36.0	35.0	38.0	34.0	39.0
5	35.722695766399525	36.0	35.0	38.0	33.0	39.0
6	35.64779492736577	36.0	35.0	37.0	33.0	39.0
7	35.60237928552454	35.0	34.0	38.0	33.0	39.0
8	35.77999679225711	36.0	35.0	38.0	33.0	39.0
9	35.75611040616091	36.0	35.0	38.0	33.0	39.0
10-14	35.68278344243991	36.0	35.0	37.8	33.0	39.0
15-19	35.68833977812716	36.0	35.0	38.0	33.0	39.0
20-24	35.67130321436056	36.0	35.0	38.0	33.0	39.0
25-29	35.656532798618905	36.0	35.0	38.0	33.0	39.0
30-34	35.6313817157126	36.0	35.0	38.0	33.0	39.0
35-39	35.6099284484744	36.0	35.0	38.0	33.0	39.0
40-44	35.59259942452617	36.0	35.0	38.0	33.0	39.0
45-49	35.56147917893675	36.0	35.0	38.0	33.0	39.0
50-54	35.543136241488675	36.0	35.0	38.0	33.0	39.0
55-59	35.51416174312963	36.0	35.0	38.0	33.0	39.0
60-64	35.478535718083194	36.0	34.8	38.0	33.0	39.0
65-69	35.44320321928284	36.0	34.0	38.0	33.0	39.0
70-74	35.41582652058968	36.0	34.0	38.0	33.0	39.0
75-79	35.372908397094335	36.0	34.0	38.0	32.4	39.0
80-84	35.336113815485405	36.0	34.0	38.0	32.0	39.0
85-89	35.29374853895952	36.0	34.0	38.0	32.0	39.0
90-94	35.240763435582004	36.0	34.0	38.0	32.0	39.0
95-99	35.180091952948466	36.0	34.0	38.0	32.0	39.0
100-104	35.13136968945926	36.0	34.0	38.0	32.0	39.0
105-109	35.0690111403188	36.0	34.0	38.0	32.0	39.0
110-114	35.00986971473816	36.0	34.0	37.8	31.6	39.0
115-119	34.9424083903351	35.4	34.0	37.0	31.0	39.0
120-124	34.86847810482054	35.0	34.0	37.0	31.0	39.0
125-129	34.809418102807236	35.0	34.0	37.0	31.0	39.0
130-134	34.72139206290705	35.0	34.0	37.0	30.8	39.0
135-139	34.63279771841839	35.0	34.0	37.0	30.0	39.0
140-144	34.53740823124541	35.0	34.0	37.0	30.0	39.0
145-149	34.5035253655996	35.0	34.0	37.0	30.0	39.0
150	35.185616072659336	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	118.0
22	1072.0
23	6189.0
24	22801.0
25	58830.0
26	118591.0
27	207196.0
28	304500.0
29	399007.0
30	512010.0
31	668766.0
32	906024.0
33	1303834.0
34	2059855.0
35	3780688.0
36	1.1296601E7
37	1896943.0
38	2.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.146513741601865	19.2855262410104	16.949416969792424	30.61854304759531
2	24.806204875981784	25.330143316708597	32.45755124244187	17.406100564867746
3	20.4342354515565	29.295952254921776	30.137644961137973	20.13216733238375
4	24.059031643924907	33.48936250102684	22.482115091878132	19.969490763170118
5	25.743649936692524	35.04006471893405	22.318169455906553	16.898115888466876
6	19.26916896097589	39.30481092623831	22.47777410507074	18.948246007715063
7	20.70668634750671	21.199294925169582	37.68200129524518	20.412017432078528
8	21.719397836294075	23.715274918061745	28.30027465025428	26.2650525953899
9	23.788563002420418	23.22248043497541	28.81128055213959	24.177676010464584
10-14	23.189999034703792	29.530438156723697	25.590740530488425	21.688822278084082
15-19	23.109170407766225	28.482651203601467	27.03936397525794	21.36881441337437
20-24	23.182867594720374	28.52814722180667	26.94989014584951	21.339095037623444
25-29	23.296568516161244	28.4128454167194	26.871650670611537	21.41893539650782
30-34	23.258271639925425	28.211668045843197	27.102871278187852	21.42718903604353
35-39	23.354435314959282	28.139093951434585	27.072149894292725	21.43432083931341
40-44	23.470701354804145	27.909498835196857	27.2191995305278	21.400600279471202
45-49	23.473535314097578	27.724413089663802	27.29858012353038	21.50347147270823
50-54	23.50834855878556	27.696125787290214	27.272589884083587	21.522935769840636
55-59	23.670834438755854	27.586142890504256	27.19332945787701	21.54969321286288
60-64	23.560190846095473	27.55712184480017	27.258440707135044	21.62424660196931
65-69	23.664012460044333	27.471910561453555	27.230422530741176	21.633654447760932
70-74	23.742202984886923	27.41424401541624	27.137578525517537	21.7059744741793
75-79	23.674854065183464	27.496829542812957	27.20016357489678	21.6281528171068
80-84	23.79744077427516	27.517943827974666	27.047197807038632	21.637417590711543
85-89	23.802706618229674	27.51099152860057	27.102305562647388	21.583996290522364
90-94	23.76349570788136	27.436131124173617	27.13781866539228	21.662554502552737
95-99	23.80122642443288	27.539441720457614	27.15677451611085	21.502557338998663
100-104	24.014769910140853	27.507777181569974	26.946139350207993	21.53131355808118
105-109	23.77120875775996	27.643506456732197	27.085841161154416	21.49944362435342
110-114	23.821285526050673	27.635328811682275	27.000244545831052	21.543141116435997
115-119	23.876524487236534	27.63503192322511	27.04755581410458	21.440887775433776
120-124	23.85101812520274	27.560124637240165	27.18000108905799	21.408856148499115
125-129	23.809023399217097	27.669722375999196	27.141073884928357	21.38018033985535
130-134	23.897725552169298	27.559071918201735	27.208333698352842	21.334868831276125
135-139	23.774963901599634	27.54687485115414	27.32368201312844	21.354479234117786
140-144	23.817975325400223	27.670982143623817	27.265313389508027	21.245729141467933
145-149	23.603095249897535	27.912166462041316	27.074786825161656	21.40995146289949
150	23.869952453528402	26.588181837393627	27.42027144932917	22.121594259748807
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	2.0
2	13.0
3	25.5
4	37.5
5	46.5
6	42.5
7	37.5
8	36.5
9	36.5
10	28.0
11	18.5
12	20.5
13	24.0
14	28.5
15	44.5
16	70.5
17	131.0
18	236.5
19	404.5
20	650.0
21	1022.0
22	1527.0
23	2509.0
24	4240.0
25	6914.0
26	10618.0
27	16638.5
28	26339.5
29	39498.0
30	54722.5
31	73128.0
32	99295.5
33	134758.0
34	180249.5
35	248756.0
36	350901.5
37	472244.0
38	615708.5
39	800685.0
40	1015746.0
41	1215528.5
42	1391338.5
43	1551386.5
44	1663595.5
45	1713649.0
46	1704633.5
47	1644380.5
48	1536792.0
49	1385555.0
50	1202797.0
51	1008051.0
52	821538.0
53	652651.5
54	508025.5
55	383880.5
56	282846.0
57	209790.0
58	154644.5
59	111087.0
60	77296.5
61	52245.0
62	35813.5
63	24564.5
64	16314.0
65	10735.0
66	7429.0
67	5595.0
68	4818.0
69	3576.0
70	1984.5
71	1256.0
72	661.0
73	424.5
74	372.0
75	262.5
76	172.0
77	52.0
78	12.5
79	2.0
80	0.0
81	0.0
82	0.5
83	0.5
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.247540907429348E-6
3	0.0
4	0.0
5	4.247540907429348E-6
6	0.0
7	4.247540907429348E-6
8	4.247540907429348E-6
9	8.495081814858696E-6
10-14	0.09681165137849268
15-19	8.495081814858698E-7
20-24	0.0
25-29	8.495081814858698E-7
30-34	0.0
35-39	8.495081814858698E-7
40-44	0.0
45-49	0.0
50-54	1.6990163629717395E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	1.6990204620340031E-6
80-84	0.0
85-89	0.0
90-94	8.495299907363852E-7
95-99	8.495408308268056E-6
100-104	8.495543416330205E-7
105-109	8.495711369148709E-7
110-114	8.495894558608074E-7
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62-63	2.0
64-65	0.0
66-67	3.0
68-69	3.0
70-71	11.0
72-73	7.0
74-75	11.0
76-77	36.0
78-79	33.0
80-81	35.0
82-83	58.0
84-85	62.0
86-87	96.0
88-89	126.0
90-91	118.0
92-93	132.0
94-95	114.0
96-97	111.0
98-99	147.0
100-101	175.0
102-103	173.0
104-105	193.0
106-107	196.0
108-109	205.0
110-111	192.0
112-113	227.0
114-115	202.0
116-117	206.0
118-119	259.0
120-121	297.0
122-123	297.0
124-125	328.0
126-127	383.0
128-129	453.0
130-131	570.0
132-133	630.0
134-135	904.0
136-137	1346.0
138-139	3123.0
140-141	6366.0
142-143	8955.0
144-145	62113.0
146-147	803714.0
148-149	836185.0
150-151	2.1814237E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.93140705441974
#Duplication Level	Percentage of total
1	25.678598442791333
2	11.49010657380293
3	7.6555651740624455
4	5.482308783577984
5	4.08237836981478
6	3.273199175677121
7	2.684989304743505
8	2.3537850231975974
9	1.9586095558843692
>10	22.009016710823303
>50	5.473522818376186
>100	5.928828915852708
>500	0.9203843466391011
>1k	0.8965955720857437
>5k	0.06672576665967409
>10k+	0.04538546601115592
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.247540907429348E-6	4.247540907429348E-6	0.0	0.0	0.0017457393129534622	0.0
2	1.6990163629717393E-5	1.2742622722288044E-5	0.0	0.0	0.005627991702343887	0.0
3	5.097049088915218E-5	1.2742622722288044E-5	0.0	0.0	0.007089145774499582	0.0
4	1.061885226857337E-4	1.6990163629717393E-5	0.0	0.0	0.008469596569414121	0.0
5	1.061885226857337E-4	1.6990163629717393E-5	0.0	0.0	0.010147375227848712	0.0
6	1.146836045005924E-4	2.1237704537146742E-5	0.0	0.0	0.011791173559023871	0.0
7	1.2742622722288044E-4	3.3980327259434785E-5	0.0	0.0	0.013825745653682529	0.0
8	1.9113934083432067E-4	3.8227868166864135E-5	0.0	0.0	0.01896527015167204	4.247540907429348E-6
9	1.9963442264917937E-4	5.097049088915218E-5	0.0	0.0	0.02489908479935084	4.247540907429348E-6
10-11	2.3998606126975818E-4	5.097049088915218E-5	0.0	0.0	0.0310304100992251	4.247540907429348E-6
12-13	2.718426180754783E-4	5.309426134286685E-5	0.0	0.0	0.0368920165514776	4.247540907429348E-6
14-15	3.270606498720598E-4	6.158934315772555E-5	0.0	0.0	0.04555487623217976	4.247540907429348E-6
16-17	3.695360589463533E-4	7.008442497258424E-5	0.0	0.0	0.05100447121641161	4.247540907429348E-6
18-19	3.9714507484464405E-4	7.645573633372827E-5	0.0	8.495081814858696E-6	0.058913392386045066	4.247540907429348E-6
20-21	4.247540907429348E-4	8.495081814858697E-5	0.0	8.495081814858696E-6	0.06452439392475923	4.247540907429348E-6
22-23	4.778483520858017E-4	9.7693440870875E-5	8.495081814858696E-6	8.495081814858696E-6	0.06989753317265734	4.247540907429348E-6
24-25	5.224475316138098E-4	1.1043606359316305E-4	8.495081814858696E-6	8.495081814858696E-6	0.07519846422512919	4.247540907429348E-6
26-27	5.500565475121006E-4	1.1893114540802175E-4	8.495081814858696E-6	8.495081814858696E-6	0.0787472846532864	4.247540907429348E-6
28-29	6.668639224664077E-4	1.1893114540802175E-4	8.495081814858696E-6	8.495081814858696E-6	0.08249786327454652	4.247540907429348E-6
30-31	8.176516246801496E-4	1.3592130903773914E-4	1.0618852268573371E-5	8.495081814858696E-6	0.08712343532273709	4.247540907429348E-6
32-33	9.429540814493154E-4	1.4016884994516848E-4	1.2742622722288044E-5	8.495081814858696E-6	0.09164494261869563	4.247540907429348E-6
34-35	0.001055513915496193	1.5291147266745654E-4	1.2742622722288044E-5	8.495081814858696E-6	0.09446743355168243	4.247540907429348E-6
36-37	0.0010979893245704865	1.5715901357488588E-4	1.6990163629717393E-5	8.495081814858696E-6	0.0978081244753756	4.247540907429348E-6
38-39	0.0011617024381819266	1.635303249360299E-4	1.6990163629717393E-5	8.495081814858696E-6	0.10091307687870646	4.247540907429348E-6
40-41	0.0012296630927007965	1.6777786584345924E-4	1.6990163629717393E-5	1.0618852268573371E-5	0.10355292355267379	6.371311361144022E-6
42-43	0.0012445294858767992	1.7839671811203264E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.10596765055854737	8.495081814858696E-6
44-45	0.0012593958790528019	1.89015570380606E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.10814663904405863	1.2742622722288044E-5
46-47	0.0013209852222105273	2.1874835673261144E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.11023430540006016	1.2742622722288044E-5
48-49	0.0013762032540071089	2.3998606126975818E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.11239417995148798	1.2742622722288044E-5
50-51	0.0014250499744425465	2.442336021771875E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.11409956762582087	1.2742622722288044E-5
52-53	0.0014484114494334077	2.463573726309022E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.11594724792055264	1.2742622722288044E-5
54-55	0.001486639317600272	2.484811430846169E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.1178374036243587	1.2742622722288044E-5
56-57	0.0015737139062025735	2.527286839920462E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.11982737653948936	1.2742622722288044E-5
58-59	0.0016437983311751577	2.569762248994756E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.12169417076830455	1.2742622722288044E-5
60-61	0.0017245016084163154	2.6971884762176364E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.12301303222006135	1.2742622722288044E-5
62-63	0.0017712245583980382	2.782139294366223E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.12479699940118168	1.2742622722288044E-5
64-65	0.001805204885657473	3.0369917488119844E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.12633036166876366	1.2742622722288044E-5
66-67	0.0018604229174540546	3.440508135017772E-4	1.6990163629717393E-5	2.1237704537146742E-5	0.12755577722055705	1.2742622722288044E-5
68-69	0.001877413081083772	3.546696657703506E-4	1.6990163629717393E-5	2.1237704537146742E-5	0.12886189604959158	1.4866393176002718E-5
70-71	0.0018859081628986307	3.567934362240653E-4	1.6990163629717393E-5	2.1237704537146742E-5	0.13009155914229237	1.6990163629717393E-5
72-73	0.0019262598015192096	3.6528851803892395E-4	1.6990163629717393E-5	2.1237704537146742E-5	0.13100053289648225	2.7609015898290764E-5
74-75	0.0019538688174175	3.737835998537827E-4	1.6990163629717393E-5	2.1237704537146742E-5	0.13210701730286759	2.973278635200544E-5
76-77	0.001981477833315791	3.8227868166864135E-4	1.6990163629717393E-5	2.1237704537146742E-5	0.13314341728428036	2.973278635200544E-5
78-79	0.002038819635566087	4.077639271132175E-4	2.1237704537146742E-5	2.1237704537146742E-5	0.1344389172610463	2.973278635200544E-5
80-81	0.00205368602874209	4.3112540210407885E-4	2.1237704537146742E-5	2.1237704537146742E-5	0.13563884756739508	2.973278635200544E-5
82-83	0.002066428651464378	4.438680248263669E-4	2.1237704537146742E-5	2.1237704537146742E-5	0.1371127442622731	2.973278635200544E-5
84-85	0.0020897901264552393	4.544868770949403E-4	2.1237704537146742E-5	2.1237704537146742E-5	0.13854416554807678	3.185655680572011E-5
86-87	0.002096161437816383	4.6298195890979895E-4	2.1237704537146742E-5	2.1237704537146742E-5	0.13993098765435247	3.3980327259434785E-5
88-89	0.0021089040605386713	4.69353270270943E-4	2.1237704537146742E-5	2.1237704537146742E-5	0.14128170566291498	3.3980327259434785E-5
90-91	0.00211739914235353	4.7997212253951635E-4	2.1237704537146742E-5	2.1237704537146742E-5	0.14214608023757686	3.3980327259434785E-5
92-93	0.002123770453714674	4.82095892993231E-4	2.548524544457609E-5	2.1237704537146742E-5	0.14305505399176674	3.610409771314946E-5
94-95	0.0021365130764369623	4.88467204354375E-4	2.548524544457609E-5	2.1237704537146742E-5	0.14411269167771665	4.035163862057881E-5
96-97	0.0021535032400666794	5.033335975303777E-4	2.548524544457609E-5	2.3361474990861417E-5	0.14500679903873054	4.2475409074293485E-5
98-99	0.002168369633242682	5.139524497989512E-4	2.548524544457609E-5	2.548524544457609E-5	0.14591152525201295	5.309426134286685E-5
100-101	0.0021747409446038262	5.224475316138098E-4	2.548524544457609E-5	2.548524544457609E-5	0.14677802359712855	5.521803179658153E-5
102-103	0.0021747409446038262	5.288188429749539E-4	2.9732786352005435E-5	2.548524544457609E-5	0.14766788341723502	5.946557270401087E-5
104-105	0.0021789884855112555	5.309426134286685E-4	3.3980327259434785E-5	2.548524544457609E-5	0.1484664211078317	6.58368840651549E-5
106-107	0.0022044737309558316	5.500565475121006E-4	3.3980327259434785E-5	2.548524544457609E-5	0.14922035961890043	7.008442497258424E-5
108-109	0.0022087212718632614	5.691704815955327E-4	3.3980327259434785E-5	2.548524544457609E-5	0.14992545140953373	7.220819542629892E-5
110-111	0.002221463894585549	5.8616064522525E-4	3.3980327259434785E-5	2.548524544457609E-5	0.15079832106601043	7.645573633372827E-5
112-113	0.002236330287761552	6.010270384012527E-4	3.3980327259434785E-5	2.973278635200544E-5	0.15156712597025515	7.857950678744294E-5
114-115	0.002244825369576411	6.031508088549675E-4	3.3980327259434785E-5	2.973278635200544E-5	0.15239114890629646	8.707458860230164E-5
116-117	0.002255444221844984	6.031508088549675E-4	3.3980327259434785E-5	2.973278635200544E-5	0.15319818167870802	9.344589996344567E-5
118-119	0.002261815533206128	6.031508088549675E-4	4.2475409074293485E-5	2.973278635200544E-5	0.15394999641932303	9.344589996344567E-5
120-121	0.0022639393036598425	6.031508088549675E-4	4.2475409074293485E-5	2.973278635200544E-5	0.1548887029598649	9.344589996344567E-5
122-123	0.0022639393036598425	6.031508088549675E-4	4.2475409074293485E-5	2.973278635200544E-5	0.15562140376639647	9.344589996344567E-5
124-125	0.0022639393036598425	6.031508088549675E-4	4.2475409074293485E-5	2.973278635200544E-5	0.15640932260472462	9.344589996344567E-5
126-127	0.002268186844567272	6.031508088549675E-4	4.2475409074293485E-5	2.973278635200544E-5	0.15738413324297962	9.7693440870875E-5
128-129	0.0022724343854747015	6.052745793086821E-4	4.2475409074293485E-5	2.973278635200544E-5	0.15828673568580837	9.7693440870875E-5
130-131	0.0022745581559284163	6.158934315772554E-4	4.459917952800816E-5	2.973278635200544E-5	0.15922544222635027	1.0194098177830436E-4
132-133	0.0022915483195581334	6.243885133921142E-4	4.6722949981722834E-5	2.973278635200544E-5	0.16004097008057672	1.0831229313944837E-4
134-135	0.002306414712734136	6.265122838458289E-4	5.097049088915218E-5	2.973278635200544E-5	0.16085862170525683	1.1043606359316305E-4
136-137	0.0023127860240952803	6.307598247532581E-4	5.521803179658153E-5	2.973278635200544E-5	0.16164016923222385	1.1255983404687774E-4
138-139	0.0023149097945489947	6.413786770218316E-4	5.521803179658153E-5	2.973278635200544E-5	0.16235375610467198	1.146836045005924E-4
>>END_MODULE
