##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.4d.R.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24010193
Total Bases	3.5 Gbp
Sequences flagged as poor quality	0
Sequence length	65-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.19271277827713	37.0	35.0	38.0	33.0	39.0
2	35.54785577941835	35.0	34.0	38.0	33.0	39.0
3	35.64481743232968	36.0	35.0	38.0	33.0	39.0
4	35.75422334172824	36.0	35.0	38.0	33.0	39.0
5	35.681953660264206	36.0	35.0	38.0	33.0	39.0
6	35.61003029005223	36.0	35.0	37.0	33.0	39.0
7	35.56031981917013	35.0	34.0	38.0	33.0	39.0
8	35.73392408799047	36.0	35.0	38.0	33.0	39.0
9	35.709403210544785	36.0	35.0	38.0	33.0	39.0
10-14	35.6307840757465	36.0	35.0	37.8	33.0	39.0
15-19	35.642810792899496	36.0	35.0	38.0	33.0	39.0
20-24	35.62310190509506	36.0	35.0	38.0	33.0	39.0
25-29	35.60538767014492	36.0	35.0	38.0	33.0	39.0
30-34	35.5771149194844	36.0	35.0	38.0	33.0	39.0
35-39	35.55774360497644	36.0	35.0	38.0	33.0	39.0
40-44	35.5338588906803	36.0	35.0	38.0	33.0	39.0
45-49	35.500310064146504	36.0	35.0	38.0	33.0	39.0
50-54	35.47975641012132	36.0	34.8	38.0	33.0	39.0
55-59	35.44714165354689	36.0	34.0	38.0	33.0	39.0
60-64	35.40912564093092	36.0	34.0	38.0	33.0	39.0
65-69	35.37048995266541	36.0	34.0	38.0	32.4	39.0
70-74	35.33707830697521	36.0	34.0	38.0	32.0	39.0
75-79	35.291036443163115	36.0	34.0	38.0	32.0	39.0
80-84	35.251359654761345	36.0	34.0	38.0	32.0	39.0
85-89	35.20649981977527	36.0	34.0	38.0	32.0	39.0
90-94	35.149555649850164	36.0	34.0	38.0	32.0	39.0
95-99	35.08428918374044	36.0	34.0	37.6	32.0	39.0
100-104	35.02991343407174	36.0	34.0	37.4	31.8	39.0
105-109	34.96416716434278	35.4	34.0	37.0	31.2	39.0
110-114	34.90091490235494	35.0	34.0	37.0	31.0	39.0
115-119	34.82755084542531	35.0	34.0	37.0	31.0	39.0
120-124	34.74938731701708	35.0	34.0	37.0	31.0	39.0
125-129	34.68553625983225	35.0	34.0	37.0	30.4	39.0
130-134	34.59201082941906	35.0	34.0	37.0	30.0	39.0
135-139	34.49781180287446	35.0	34.0	37.0	30.0	39.0
140-144	34.40179074463085	35.0	34.0	37.0	30.0	39.0
145-149	34.37072564311255	35.0	34.0	37.0	30.0	39.0
150	35.08345597321415	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	122.0
22	1093.0
23	6518.0
24	23832.0
25	61527.0
26	127025.0
27	226286.0
28	332699.0
29	433550.0
30	551813.0
31	719900.0
32	976721.0
33	1401641.0
34	2210686.0
35	4031025.0
36	1.1154573E7
37	1751177.0
38	2.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.200399513656556	19.183969075134048	16.975869373478172	30.639762037731227
2	25.116245421267543	25.06361777266847	32.47305842147958	17.347078384584414
3	20.39028675822334	29.233389720498693	30.409444455921054	19.966879065356913
4	24.02567109727106	33.37391748579447	22.642004585302583	19.958406831631883
5	25.777074169169495	35.01924099565718	22.40527272751505	16.79841210765828
6	19.17992079447258	39.353544555014615	22.56066413127125	18.905870519241557
7	20.580680904176027	21.15128025631782	37.98974202288761	20.278296816618543
8	21.58323521874524	23.663868396548782	28.506337163248723	26.246559221457254
9	23.68852853999013	23.194975532677027	28.950274314814788	24.166221612518054
10-14	23.092090185980435	29.55318967666547	25.709541648551838	21.645178488802262
15-19	23.033255276484077	28.471441512123135	27.123260751158984	21.372042460233807
20-24	23.110369833345363	28.546565202537106	27.023191358770006	21.319873605347528
25-29	23.23562996765582	28.41869451028569	26.941791763189908	21.403883758868577
30-34	23.192344184821838	28.237677223169342	27.167779950790067	21.402198641218753
35-39	23.307733705495277	28.164220322295787	27.112328727322836	21.415717244886096
40-44	23.410150847183946	27.951567069869032	27.261326054313685	21.37695602863334
45-49	23.43662729771999	27.770111033843925	27.336754247137907	21.456507421298173
50-54	23.4771640020441	27.74142807569862	27.303312465778596	21.478095456478684
55-59	23.636401423345493	27.616892542263194	27.248556477659303	21.49814955673201
60-64	23.535685864749194	27.590583715841017	27.303646413837658	21.57008400557213
65-69	23.644133504591743	27.515159356267276	27.272893035691464	21.567814103449518
70-74	23.710713066469097	27.451000432683298	27.18175703803395	21.65652946281366
75-79	23.633280194920424	27.542659063574604	27.252331560006542	21.57172918149843
80-84	23.76415012455625	27.57591450508446	27.095141381712214	21.56479398864708
85-89	23.76456296266037	27.572768655189122	27.16041396286471	21.502254419285794
90-94	23.721101470360175	27.488974758107666	27.200033506616062	21.589890264916097
95-99	23.762996443872073	27.59494461720186	27.222262577022367	21.4197963619037
100-104	23.974401952135047	27.540817189648404	27.03317564302215	21.4516052151944
105-109	23.728375505571954	27.704981447873735	27.156086657372235	21.410556389182076
110-114	23.760942139875883	27.70661038846855	27.061401242693766	21.4710462289618
115-119	23.822133643569295	27.695123553991465	27.115597974553356	21.367144827885888
120-124	23.80152718804154	27.613325616628714	27.26781427446338	21.31733292086637
125-129	23.7529025311327	27.720151988084535	27.23673137521816	21.2902141055646
130-134	23.82408676122661	27.609459733488535	27.332784361830875	21.23366914345398
135-139	23.702391781056605	27.610616290762824	27.43967967372744	21.24731225445313
140-144	23.735858685441144	27.716524264395453	27.407471884773667	21.140145165389736
145-149	23.5004992991489	27.952630260596408	27.232771272853935	21.31409916740076
150	23.74367880344817	26.673718910474747	27.589169137941383	21.993433148135697
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	3.0
2	13.5
3	33.0
4	49.0
5	50.5
6	47.0
7	44.0
8	36.0
9	27.5
10	19.0
11	16.0
12	23.0
13	22.0
14	23.5
15	44.0
16	70.0
17	123.5
18	226.5
19	433.0
20	724.5
21	1149.5
22	1742.5
23	2911.0
24	5069.5
25	8110.5
26	12193.0
27	19063.0
28	30168.0
29	44131.0
30	60895.0
31	81530.0
32	110043.5
33	147063.5
34	194054.5
35	266214.0
36	371036.5
37	495282.5
38	642725.0
39	831700.5
40	1051177.0
41	1251312.0
42	1425216.5
43	1584273.5
44	1693764.0
45	1738539.0
46	1723758.0
47	1657802.0
48	1545558.0
49	1394694.5
50	1211441.5
51	1013877.0
52	826983.5
53	656622.0
54	510360.0
55	386542.0
56	285147.5
57	211950.5
58	156606.0
59	112422.5
60	78342.5
61	52844.5
62	36047.0
63	24594.0
64	16317.5
65	10825.0
66	7342.5
67	5406.0
68	4742.0
69	3497.0
70	1965.5
71	1232.5
72	650.5
73	448.5
74	391.5
75	260.5
76	181.0
77	71.0
78	16.5
79	4.0
80	1.5
81	0.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	4.164897799863583E-6
4	0.0
5	4.164897799863583E-6
6	0.0
7	4.164897799863583E-6
8	8.329795599727166E-6
9	2.4989386799181498E-5
10-14	0.10704786921121374
15-19	8.329795599727166E-7
20-24	0.0
25-29	0.0
30-34	0.0
35-39	8.329795599727166E-7
40-44	0.0
45-49	8.329795599727166E-7
50-54	3.3319182398908665E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	8.329803093367339E-7
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	6.664094992409096E-6
100-104	8.330268141751676E-7
105-109	0.0
110-114	0.0
115-119	8.330837900263959E-7
120-124	0.0
125-129	8.331419328599576E-7
130-134	0.0
135-139	0.0
140-144	8.3367918514329E-7
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
64-65	4.0
66-67	3.0
68-69	4.0
70-71	8.0
72-73	19.0
74-75	12.0
76-77	30.0
78-79	25.0
80-81	45.0
82-83	68.0
84-85	66.0
86-87	88.0
88-89	113.0
90-91	120.0
92-93	122.0
94-95	126.0
96-97	151.0
98-99	173.0
100-101	187.0
102-103	184.0
104-105	197.0
106-107	188.0
108-109	246.0
110-111	234.0
112-113	231.0
114-115	219.0
116-117	274.0
118-119	282.0
120-121	330.0
122-123	331.0
124-125	381.0
126-127	438.0
128-129	510.0
130-131	600.0
132-133	714.0
134-135	1051.0
136-137	1575.0
138-139	3312.0
140-141	6872.0
142-143	9771.0
144-145	65300.0
146-147	823080.0
148-149	902881.0
150-151	2.2189628E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.043753124747475
#Duplication Level	Percentage of total
1	25.912617800459824
2	11.455044889216616
3	7.347903190340864
4	5.414869979607735
5	4.082209816538087
6	3.404579981365515
7	2.669373742555936
8	2.262383342761057
9	1.9428603610350548
>10	22.200799849207726
>50	5.380618461704462
>100	5.938060716804371
>500	0.8841630508699636
>1k	0.9833306370019619
>5k	0.07565075651884628
>10k+	0.04553342401193397
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0016992783023443419	0.0
2	1.6659591199454332E-5	1.2494693399590749E-5	0.0	0.0	0.0057767132484107895	0.0
3	5.830856919809016E-5	1.2494693399590749E-5	0.0	0.0	0.007401023390357587	0.0
4	7.49681603975445E-5	1.6659591199454332E-5	0.0	0.0	0.008637998036917071	0.0
5	7.49681603975445E-5	1.6659591199454332E-5	0.0	4.164897799863583E-6	0.010378925317260049	0.0
6	8.746285379713524E-5	1.6659591199454332E-5	0.0	4.164897799863583E-6	0.011957421583408347	0.0
7	1.0828734279645316E-4	1.6659591199454332E-5	0.0	4.164897799863583E-6	0.01400655130094123	0.0
8	1.3327672959563466E-4	2.4989386799181498E-5	0.0	4.164897799863583E-6	0.01944590782756307	0.0
9	1.457714229952254E-4	2.915428459904508E-5	0.0	4.164897799863583E-6	0.025093509244178087	0.0
10-11	1.6659591199454332E-4	3.123673349897687E-5	0.0	4.164897799863583E-6	0.03137834002417223	0.0
12-13	2.0407999219331557E-4	3.3319182398908664E-5	0.0	4.164897799863583E-6	0.03746325570977293	0.0
14-15	2.4156407239208781E-4	3.3319182398908664E-5	0.0	4.164897799863583E-6	0.04635739496138161	0.0
16-17	2.7696570369092823E-4	3.748408019877225E-5	0.0	4.164897799863583E-6	0.05198417188899731	0.0
18-19	3.165322327896323E-4	4.9978773598362996E-5	0.0	4.164897799863583E-6	0.0600599087229328	0.0
20-21	3.7067590418785886E-4	5.830856919809016E-5	0.0	4.164897799863583E-6	0.06568460320164857	0.0
22-23	4.2273712668615366E-4	7.28857114976127E-5	0.0	4.164897799863583E-6	0.07142383236986058	0.0
24-25	4.706334513845849E-4	8.538040489720345E-5	0.0	4.164897799863583E-6	0.07695273419917949	0.0
26-27	4.935403892838345E-4	1.0412244499658958E-4	0.0	4.164897799863583E-6	0.080661575689958	0.0
28-29	5.747558963811744E-4	1.0828734279645316E-4	0.0	4.164897799863583E-6	0.08450161146143223	0.0
30-31	6.892905858774229E-4	1.1453468949624853E-4	2.0824488999317915E-6	4.164897799863583E-6	0.08936412964277296	0.0
32-33	7.892481330741489E-4	1.2286448509597569E-4	4.164897799863583E-6	1.2494693399590749E-5	0.0941808339483152	0.0
34-35	8.892056802708749E-4	1.3119428069570285E-4	4.164897799863583E-6	1.2494693399590749E-5	0.09719205505761658	0.0
36-37	9.246073115697154E-4	1.3744162739549824E-4	4.164897799863583E-6	1.2494693399590749E-5	0.10072597084080082	0.0
38-39	9.849983296677375E-4	1.4160652519536182E-4	4.164897799863583E-6	1.2494693399590749E-5	0.1039662613290947	4.164897799863583E-6
40-41	0.0010245648587664414	1.457714229952254E-4	1.2494693399590749E-5	1.2494693399590749E-5	0.10684628815770036	4.164897799863583E-6
42-43	0.0010349771032661004	1.49936320795089E-4	1.457714229952254E-5	1.2494693399590749E-5	0.10968050111050753	4.164897799863583E-6
44-45	0.0010599664900652818	1.5618366749488435E-4	1.6659591199454332E-5	1.457714229952254E-5	0.11203158591853052	4.164897799863583E-6
46-47	0.0011120277125635765	1.8742040099386124E-4	1.6659591199454332E-5	1.6659591199454332E-5	0.11452844214954874	8.329795599727166E-6
48-49	0.0011661713839618032	1.8742040099386124E-4	1.6659591199454332E-5	1.6659591199454332E-5	0.11672750818787671	1.0412244499658958E-5
50-51	0.0012036554641605755	1.957501965935884E-4	1.6659591199454332E-5	1.6659591199454332E-5	0.11875373096751035	1.2494693399590749E-5
52-53	0.0012328097487596206	1.9783264549352018E-4	1.6659591199454332E-5	2.0824488999317915E-5	0.12080910803174302	1.2494693399590749E-5
54-55	0.0012848709712579153	2.1240978779304273E-4	1.6659591199454332E-5	2.0824488999317915E-5	0.1225437879653862	1.2494693399590749E-5
56-57	0.0013369321937562102	2.144922366929745E-4	1.6659591199454332E-5	2.0824488999317915E-5	0.12439508503742557	1.2494693399590749E-5
58-59	0.0013848285184546415	2.1865713449283812E-4	1.6659591199454332E-5	2.4989386799181498E-5	0.12640048332805986	1.2494693399590749E-5
60-61	0.0014535493321523904	2.2906937899249707E-4	1.6659591199454332E-5	2.4989386799181498E-5	0.12784570286461255	1.457714229952254E-5
62-63	0.0014806211678515036	2.39481623492156E-4	1.6659591199454332E-5	2.707183569911329E-5	0.1297032472833517	2.0824488999317915E-5
64-65	0.001497280759050958	2.519763168917468E-4	1.6659591199454332E-5	2.915428459904508E-5	0.1314254325235953	2.0824488999317915E-5
66-67	0.0015243525947500713	2.665534591912693E-4	1.6659591199454332E-5	2.915428459904508E-5	0.13290188879364692	2.2906937899249707E-5
68-69	0.0015451770837493894	2.8113060149079184E-4	1.6659591199454332E-5	2.915428459904508E-5	0.1342554805786026	2.707183569911329E-5
70-71	0.0015618366749488436	2.89460397090519E-4	1.6659591199454332E-5	2.915428459904508E-5	0.13558200052785915	2.915428459904508E-5
72-73	0.001628475039746661	2.9779019269024617E-4	1.6659591199454332E-5	2.915428459904508E-5	0.13650036049272907	3.5401631298840456E-5
74-75	0.001668041568845365	3.0611998828997334E-4	1.8742040099386124E-5	2.915428459904508E-5	0.13756865677839408	3.956652909870404E-5
76-77	0.0017117729957439325	3.186146816895641E-4	2.0824488999317915E-5	2.915428459904508E-5	0.13877022979365472	4.164897799863583E-5
78-79	0.0017555044226425002	3.3319182398908664E-4	2.2906937899249707E-5	2.915428459904508E-5	0.1403008297351046	4.164897799863583E-5
80-81	0.0017638342182422274	3.519338640884728E-4	2.915428459904508E-5	2.915428459904508E-5	0.1416377619288608	4.373142689856762E-5
82-83	0.001786741156141477	3.79005699787586E-4	2.915428459904508E-5	2.915428459904508E-5	0.1429996835094162	4.7896324698431205E-5
84-85	0.0018117305429406587	3.79005699787586E-4	2.915428459904508E-5	3.123673349897687E-5	0.14442407855696954	4.9978773598362996E-5
86-87	0.001838802378639772	3.8317059758744964E-4	3.123673349897687E-5	3.3319182398908664E-5	0.14609420257471484	5.206122249829479E-5
88-89	0.0018429672764396355	3.89417944287245E-4	3.3319182398908664E-5	3.3319182398908664E-5	0.1477226776144615	5.414367139822658E-5
90-91	0.0018554619698392262	4.0815998438663113E-4	3.3319182398908664E-5	3.3319182398908664E-5	0.14873891267762823	6.455591589788554E-5
92-93	0.0018617093165390216	4.123248821864947E-4	3.5401631298840456E-5	3.748408019877225E-5	0.14960104652219997	6.663836479781733E-5
94-95	0.0018700391121387488	4.164897799863583E-4	3.748408019877225E-5	4.373142689856762E-5	0.15071515668366348	6.663836479781733E-5
96-97	0.0018742040099386124	4.206546777862219E-4	3.748408019877225E-5	4.581387579849941E-5	0.15187299827202555	6.663836479781733E-5
98-99	0.0018742040099386124	4.269020244860172E-4	3.956652909870404E-5	5.414367139822658E-5	0.15295795414889002	6.872081369774912E-5
100-101	0.0018825338055383394	4.3314937118581263E-4	4.164897799863583E-5	5.414367139822658E-5	0.15381384064676198	7.080326259768091E-5
102-103	0.001886698703338203	4.3314937118581263E-4	4.373142689856762E-5	5.414367139822658E-5	0.15469679898033306	7.080326259768091E-5
104-105	0.0018908636011380666	4.3314937118581263E-4	4.581387579849941E-5	5.414367139822658E-5	0.15551936629580612	7.705060929747629E-5
106-107	0.0019200178857371117	4.3314937118581263E-4	4.581387579849941E-5	5.414367139822658E-5	0.15625863565528192	8.121550709733987E-5
108-109	0.0019221003346370435	4.4356161568547155E-4	4.581387579849941E-5	5.414367139822658E-5	0.1570770380729551	8.329795599727166E-5
110-111	0.001926265232436907	4.560563090850623E-4	4.581387579849941E-5	5.414367139822658E-5	0.15796207885542612	8.538040489720345E-5
112-113	0.0019304301302367707	4.706334513845849E-4	4.581387579849941E-5	5.414367139822658E-5	0.15868468862370244	8.746285379713524E-5
114-115	0.0019387599258364977	4.8312814478417563E-4	4.581387579849941E-5	5.414367139822658E-5	0.15945935961447705	8.746285379713524E-5
116-117	0.0019450072725362931	4.872930425840392E-4	4.581387579849941E-5	5.414367139822658E-5	0.16041520365954576	8.954530269706703E-5
118-119	0.001961666863735748	4.914579403839027E-4	4.9978773598362996E-5	5.414367139822658E-5	0.1611461432234218	9.371020049693062E-5
120-121	0.0019679142104355426	4.914579403839027E-4	4.9978773598362996E-5	5.414367139822658E-5	0.16203743135259263	1.0203999609665778E-4
122-123	0.001978326454935202	4.977052870836982E-4	4.9978773598362996E-5	5.414367139822658E-5	0.1628100198944673	1.0828734279645316E-4
124-125	0.001984573801634997	5.060350826834254E-4	5.414367139822658E-5	5.414367139822658E-5	0.16363050476104046	1.0828734279645316E-4
126-127	0.00199915094393452	5.081175315833571E-4	5.622612029815837E-5	5.414367139822658E-5	0.16461133819290832	1.0828734279645316E-4
128-129	0.0020158105351339743	5.122824293832207E-4	5.830856919809016E-5	5.414367139822658E-5	0.16558384182917646	1.1245224059631674E-4
130-131	0.0020241403307337015	5.268595716827432E-4	5.830856919809016E-5	5.414367139822658E-5	0.16668545729724038	1.1661713839618032E-4
132-133	0.0020387174730332235	5.331069183825386E-4	5.830856919809016E-5	5.414367139822658E-5	0.1676017348132104	1.1869958729611212E-4
134-135	0.002059541962032542	5.414367139822657E-4	5.830856919809016E-5	5.830856919809016E-5	0.1684284670264833	1.2286448509597569E-4
136-137	0.0020699542065322008	5.49766509581993E-4	6.0391018098021954E-5	5.830856919809016E-5	0.16930101311555473	1.270293828958393E-4
138-139	0.0020741191043320644	5.622612029815837E-4	6.247346699795375E-5	6.455591589788554E-5	0.17011108573762818	1.3327672959563466E-4
>>END_MODULE
