##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.4d.R.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24023071
Total Bases	3.5 Gbp
Sequences flagged as poor quality	0
Sequence length	57-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.08109966456828	37.0	35.0	38.0	33.0	39.0
2	35.42126587395924	35.0	34.0	37.0	33.0	39.0
3	35.543082147990155	35.0	34.0	37.0	33.0	39.0
4	35.63524480279811	36.0	35.0	38.0	33.0	39.0
5	35.55865909067163	36.0	35.0	37.0	33.0	39.0
6	35.49893650149891	36.0	35.0	37.0	33.0	39.0
7	35.4625519776385	35.0	34.0	37.0	33.0	39.0
8	35.61928223081887	36.0	35.0	38.0	33.0	39.0
9	35.59634682010472	36.0	35.0	38.0	33.0	39.0
10-14	35.517236701335975	36.0	34.8	37.8	33.0	39.0
15-19	35.52362776599212	36.0	35.0	38.0	33.0	39.0
20-24	35.50440726749715	36.0	34.8	38.0	33.0	39.0
25-29	35.48377909718537	36.0	34.2	38.0	33.0	39.0
30-34	35.45697234962174	36.0	34.0	38.0	33.0	39.0
35-39	35.43143656362669	36.0	34.0	38.0	33.0	39.0
40-44	35.41039074479694	36.0	34.0	38.0	33.0	39.0
45-49	35.37317129021515	36.0	34.0	38.0	33.0	39.0
50-54	35.3517545779222	36.0	34.0	38.0	32.2	39.0
55-59	35.31499502799965	36.0	34.0	38.0	32.0	39.0
60-64	35.2758765847102	36.0	34.0	38.0	32.0	39.0
65-69	35.233172136776965	36.0	34.0	37.8	32.0	39.0
70-74	35.19976771596938	36.0	34.0	37.8	32.0	39.0
75-79	35.14856656838417	36.0	34.0	37.0	32.0	39.0
80-84	35.105802557386255	35.8	34.0	37.0	32.0	39.0
85-89	35.05764900054685	35.2	34.0	37.0	32.0	39.0
90-94	34.99667402077632	35.0	34.0	37.0	31.8	39.0
95-99	34.92749087777518	35.0	34.0	37.0	31.0	39.0
100-104	34.86876348541852	35.0	34.0	37.0	31.0	39.0
105-109	34.79859997905179	35.0	34.0	37.0	31.0	39.0
110-114	34.7298426337327	35.0	34.0	37.0	31.0	39.0
115-119	34.65293980604642	35.0	34.0	37.0	30.2	39.0
120-124	34.56878778003639	35.0	34.0	37.0	30.0	39.0
125-129	34.49928341541266	35.0	34.0	37.0	30.0	39.0
130-134	34.399079684173486	35.0	34.0	37.0	30.0	39.0
135-139	34.295136101613295	35.0	34.0	37.0	29.4	39.0
140-144	34.18719282375082	35.0	34.0	37.0	29.0	39.0
145-149	34.1522965345611	35.0	34.0	37.0	29.0	39.0
150	34.931026473457926	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	10.0
21	170.0
22	1443.0
23	8140.0
24	29563.0
25	75114.0
26	149114.0
27	253520.0
28	365411.0
29	475467.0
30	608206.0
31	795363.0
32	1075673.0
33	1537056.0
34	2386271.0
35	4215009.0
36	1.0524007E7
37	1523527.0
38	5.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.00804464175292	19.01129543346061	16.794771992306895	31.185887932479574
2	24.748510494287366	25.115703363475195	32.99955417854587	17.136231963691568
3	20.121470736254775	29.317256287393747	30.38332735990862	20.177945616442862
4	23.792933209618923	33.672715435620844	22.559556293179845	19.974795061580387
5	25.53276186319075	35.18834333781417	22.44556680081134	16.833327998183744
6	18.990544547780754	39.28278362079519	22.672230373876843	19.054441457547206
7	20.402254149771277	20.971827457030784	38.12412243214034	20.5017959610576
8	21.39445125040222	23.592700683135003	28.566465485052493	26.446382581410287
9	23.575237083591364	23.21834260379826	28.953155731530867	24.25326458107951
10-14	23.03066773202481	29.503721832285663	25.70874722154628	21.756863214143245
15-19	22.955066818892554	28.46109059079083	27.145290458493005	21.438552131823613
20-24	23.037680927951975	28.519241595042043	27.066288294833647	21.37678918217233
25-29	23.146357932339292	28.418207647140537	26.978430859235274	21.4570035612849
30-34	23.123857062238212	28.196997794328627	27.200696363924497	21.478448779508668
35-39	23.21800489204732	28.13682563732172	27.176393059821535	21.468776410809426
40-44	23.33802868084601	27.91007694228602	27.307535327186105	21.444359049681864
45-49	23.34716759024829	27.728508380369092	27.394202243286898	21.530121786095723
50-54	23.39464464630096	27.70072282100357	27.347822055686756	21.556810477008714
55-59	23.55693863714491	27.58275954948074	27.288708047171955	21.571593766202394
60-64	23.457649225642673	27.556423513837714	27.34587839682236	21.640048863697253
65-69	23.57555883394171	27.469431400568983	27.304178559784702	21.650831205704606
70-74	23.627204385469145	27.423790633297084	27.215477318936315	21.733527662297455
75-79	23.569276900926734	27.504990863795598	27.278159870468777	21.64757236480889
80-84	23.6930469407322	27.528106533741063	27.128843360630146	21.650003164896592
85-89	23.693609092039537	27.517490288686254	27.18222992264184	21.606670696632367
90-94	23.65635270883448	27.438533821644285	27.22155964612692	21.683553823394316
95-99	23.712204875795972	27.520633025153934	27.256030262533415	21.511131836516682
100-104	23.90742406170686	27.49554020180152	27.062962068983694	21.53407366750793
105-109	23.674532800450375	27.63946114540173	27.179464434512134	21.50654161963576
110-114	23.715347945907894	27.633138800452322	27.092053877622636	21.559459376017152
115-119	23.77360606430301	27.6289816605652	27.136218239663783	21.461194035468
120-124	23.752509499547948	27.55153014211406	27.274919180004854	21.421041178333137
125-129	23.711390169126805	27.65399250907287	27.25243202319093	21.38218529860939
130-134	23.77544790497114	27.548737427419724	27.338532055548892	21.33728261206024
135-139	23.668607512116495	27.541201939371803	27.4372539548583	21.352936593653407
140-144	23.704260025576808	27.66281014611099	27.405832106525274	21.22709772178693
145-149	23.452227330201016	27.83785990975599	27.293377338414366	21.416535421628634
150	23.669690664389517	26.559542864976688	27.671566365590188	22.099200105043607
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	2.0
2	8.5
3	26.0
4	46.0
5	52.5
6	44.0
7	39.5
8	42.5
9	30.5
10	19.5
11	18.5
12	14.5
13	18.5
14	31.5
15	55.5
16	81.0
17	134.0
18	239.0
19	446.0
20	727.0
21	1160.0
22	1839.0
23	3067.0
24	5162.5
25	8245.5
26	12639.0
27	19685.0
28	31228.0
29	46019.5
30	62652.0
31	83242.5
32	111334.0
33	148624.0
34	195214.0
35	265675.5
36	371459.0
37	495636.0
38	641311.5
39	828837.0
40	1048124.5
41	1249003.5
42	1423844.0
43	1582895.0
44	1691518.0
45	1735296.0
46	1721698.0
47	1658604.0
48	1551119.0
49	1401595.5
50	1216903.5
51	1018091.5
52	827914.0
53	657694.5
54	511359.0
55	386312.0
56	285341.0
57	211585.0
58	155508.0
59	111748.0
60	77538.0
61	51983.5
62	35454.5
63	24366.0
64	16091.0
65	10615.5
66	7400.5
67	5539.5
68	4776.5
69	3436.0
70	1831.0
71	1131.0
72	577.0
73	394.0
74	346.0
75	252.5
76	166.0
77	60.5
78	17.5
79	1.5
80	0.5
81	0.0
82	0.0
83	1.0
84	1.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.162665131364762E-6
3	4.162665131364762E-6
4	4.162665131364762E-6
5	8.325330262729524E-6
6	0.0
7	0.0
8	4.162665131364762E-6
9	1.6650660525459047E-5
10-14	0.101489106034778
15-19	0.0
20-24	8.325330262729524E-7
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	8.325330262729524E-7
50-54	1.6650660525459047E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	1.6650712370341216E-6
80-84	0.0
85-89	0.0
90-94	0.0
95-99	7.493116074262576E-6
100-104	0.0
105-109	1.6652037019543145E-6
110-114	0.0
115-119	3.3305851565011986E-6
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
56-57	1.0
58-59	0.0
60-61	0.0
62-63	1.0
64-65	1.0
66-67	3.0
68-69	7.0
70-71	13.0
72-73	16.0
74-75	19.0
76-77	25.0
78-79	28.0
80-81	49.0
82-83	82.0
84-85	75.0
86-87	90.0
88-89	129.0
90-91	128.0
92-93	140.0
94-95	144.0
96-97	147.0
98-99	177.0
100-101	194.0
102-103	192.0
104-105	208.0
106-107	225.0
108-109	239.0
110-111	260.0
112-113	260.0
114-115	259.0
116-117	307.0
118-119	329.0
120-121	391.0
122-123	394.0
124-125	465.0
126-127	547.0
128-129	602.0
130-131	771.0
132-133	904.0
134-135	1275.0
136-137	1763.0
138-139	3578.0
140-141	7336.0
142-143	11019.0
144-145	67821.0
146-147	829389.0
148-149	976539.0
150-151	2.2116529E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.79815983819302
#Duplication Level	Percentage of total
1	25.73959409937261
2	11.205146997128406
3	7.395264331959164
4	5.557351963003005
5	4.244208851338603
6	3.227026496888214
7	2.6682179330235556
8	2.334580881826242
9	1.919826749292644
>10	21.95292470251669
>50	5.500980552497141
>100	6.216401275683555
>500	0.9465788528826473
>1k	0.9769904881171779
>5k	0.0707647304709256
>10k+	0.044141093999395364
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.162665131364762E-6	4.162665131364762E-6	4.162665131364762E-6	0.0	0.001710855368990917	0.0
2	2.081332565682381E-5	4.162665131364762E-6	4.162665131364762E-6	4.162665131364762E-6	0.005640411252999252	0.0
3	3.746398618228285E-5	4.162665131364762E-6	4.162665131364762E-6	4.162665131364762E-6	0.007268013319362874	0.0
4	7.909063749593048E-5	8.325330262729524E-6	8.325330262729524E-6	4.162665131364762E-6	0.00857509017061141	0.0
5	7.909063749593048E-5	8.325330262729524E-6	8.325330262729524E-6	4.162665131364762E-6	0.010169390915924113	0.0
6	7.909063749593048E-5	8.325330262729524E-6	8.325330262729524E-6	4.162665131364762E-6	0.011817806307944559	0.0
7	9.157863289002475E-5	2.081332565682381E-5	8.325330262729524E-6	4.162665131364762E-6	0.013928277529546493	0.0
8	1.415306144664019E-4	2.081332565682381E-5	8.325330262729524E-6	4.162665131364762E-6	0.019281464888481578	0.0
9	1.4569327959776667E-4	2.9138655919553333E-5	8.325330262729524E-6	4.162665131364762E-6	0.025130009398049068	0.0
10-11	1.7691326808300236E-4	3.746398618228285E-5	1.6650660525459047E-5	4.162665131364762E-6	0.03132405511351983	0.0
12-13	2.1021458913392048E-4	3.746398618228285E-5	1.6650660525459047E-5	4.162665131364762E-6	0.0373765702145242	0.0
14-15	2.2686524965937952E-4	3.746398618228285E-5	1.6650660525459047E-5	4.162665131364762E-6	0.04605572701341973	0.0
16-17	2.6224790327598E-4	3.746398618228285E-5	1.6650660525459047E-5	4.162665131364762E-6	0.05186056353910788	0.0
18-19	3.1219988485235717E-4	3.954531874796524E-5	1.6650660525459047E-5	4.162665131364762E-6	0.05999441120579463	0.0
20-21	3.475825384689576E-4	4.7870649010694756E-5	1.6650660525459047E-5	4.162665131364762E-6	0.06584087438279644	0.0
22-23	3.8712785721692285E-4	7.49279723645657E-5	1.6650660525459047E-5	4.162665131364762E-6	0.07169982555519235	0.0
24-25	4.329171736619352E-4	8.741596775866E-5	1.6650660525459047E-5	4.162665131364762E-6	0.07687609964604442	0.0
26-27	4.641371621471709E-4	9.990396315275428E-5	1.6650660525459047E-5	4.162665131364762E-6	0.0807994115323557	0.0
28-29	5.577971276028781E-4	9.990396315275428E-5	1.6650660525459047E-5	4.162665131364762E-6	0.08483303404464816	0.0
30-31	6.805957489781386E-4	1.0406662828411904E-4	1.8731993091141427E-5	4.162665131364762E-6	0.08989067217925634	0.0
32-33	8.054757029190814E-4	1.0614796084980143E-4	2.081332565682381E-5	4.162665131364762E-6	0.09493165965333908	0.0
34-35	8.991356683747886E-4	1.1239195854684857E-4	2.081332565682381E-5	4.162665131364762E-6	0.09828468641665339	0.0
36-37	9.574129802138952E-4	1.1655462367821333E-4	2.081332565682381E-5	4.162665131364762E-6	0.1018396024388389	4.162665131364762E-6
38-39	0.0010198529571843666	1.2071728880957809E-4	2.081332565682381E-5	4.162665131364762E-6	0.10542990111464101	4.162665131364762E-6
40-41	0.001059398275932332	1.2279862137526046E-4	2.081332565682381E-5	6.243997697047143E-6	0.10830214005528269	4.162665131364762E-6
42-43	0.0010906182644175675	1.2904261907230763E-4	2.081332565682381E-5	8.325330262729524E-6	0.1112784456242085	4.162665131364762E-6
44-45	0.0011218382529028033	1.498559447291314E-4	2.081332565682381E-5	8.325330262729524E-6	0.11364283941882368	4.162665131364762E-6
46-47	0.0011634649042164508	1.977265937398262E-4	2.081332565682381E-5	8.325330262729524E-6	0.11619455314435029	4.162665131364762E-6
48-49	0.0012113355532271455	1.9980792630550857E-4	2.081332565682381E-5	8.325330262729524E-6	0.11854021494587433	4.162665131364762E-6
50-51	0.001244636874278064	2.060519240025557E-4	2.081332565682381E-5	8.325330262729524E-6	0.12048209822965597	4.162665131364762E-6
52-53	0.0012737755301976171	2.1437725426528523E-4	2.081332565682381E-5	8.325330262729524E-6	0.12251764147889335	4.162665131364762E-6
54-55	0.0013029141861171704	2.164585868309676E-4	2.081332565682381E-5	8.325330262729524E-6	0.12429718082255178	4.162665131364762E-6
56-57	0.0013632728305219594	2.2062125196233237E-4	2.081332565682381E-5	8.325330262729524E-6	0.12636186272770872	4.162665131364762E-6
58-59	0.00144236346801789	2.372719124877914E-4	2.081332565682381E-5	8.325330262729524E-6	0.12857848191016044	4.162665131364762E-6
60-61	0.0015048034449883613	2.6016657071029764E-4	2.081332565682381E-5	8.325330262729524E-6	0.13013531866929084	8.325330262729524E-6
62-63	0.001519372772948138	2.809798963671214E-4	2.081332565682381E-5	1.0406662828411905E-5	0.13228325387707507	1.4569327959776666E-5
64-65	0.0015360234334735972	2.997118894582628E-4	2.081332565682381E-5	1.2487995394094285E-5	0.13425011315164492	1.6650660525459047E-5
66-67	0.0015859754150499743	3.2260654768076903E-4	2.081332565682381E-5	1.4569327959776666E-5	0.1357195339430167	1.6650660525459047E-5
68-69	0.0016192767361008923	3.4550120590327524E-4	2.081332565682381E-5	1.6650660525459047E-5	0.13715565341333752	1.6650660525459047E-5
70-71	0.001650496724586128	3.5590786873168715E-4	2.081332565682381E-5	1.6650660525459047E-5	0.1385064382484654	1.8731993091141427E-5
72-73	0.0016942047084654581	3.704771966914638E-4	2.081332565682381E-5	1.6650660525459047E-5	0.13947633922407338	2.2894658222506192E-5
74-75	0.0017170993666879642	3.808838595198757E-4	2.081332565682381E-5	1.6650660525459047E-5	0.14065229212368394	2.9138655919553333E-5
76-77	0.0017399940249104703	4.0585985030806425E-4	2.081332565682381E-5	1.6650660525459047E-5	0.14189692899796202	2.9138655919553333E-5
78-79	0.0017753766785270709	4.4124250392466475E-4	2.081332565682381E-5	1.6650660525459047E-5	0.14336010579163672	3.3301321050918095E-5
80-81	0.001787864673921165	4.495678341873943E-4	2.081332565682381E-5	1.6650660525459047E-5	0.14472337862215867	4.162665131364762E-5
82-83	0.0018149219972750361	4.7662515754126524E-4	2.081332565682381E-5	2.081332565682381E-5	0.1463405740256939	4.162665131364762E-5
84-85	0.0018398979880632246	4.8703182036967716E-4	2.081332565682381E-5	2.2894658222506192E-5	0.1480056400782398	4.370798387933E-5
86-87	0.001862792646285731	5.016011483294538E-4	2.081332565682381E-5	2.497599078818857E-5	0.1496936007890082	4.578931644501238E-5
88-89	0.0018731993091141428	5.099264785921833E-4	2.081332565682381E-5	2.497599078818857E-5	0.15142318815109024	4.578931644501238E-5
90-91	0.0018773619742455075	5.494717973401485E-4	2.081332565682381E-5	2.497599078818857E-5	0.1524825864270226	4.578931644501238E-5
92-93	0.001889849969639602	5.619597927342428E-4	2.497599078818857E-5	2.497599078818857E-5	0.15354198470295494	4.578931644501238E-5
94-95	0.0018981752999023315	5.744477881283372E-4	2.497599078818857E-5	2.497599078818857E-5	0.15462844030224113	4.995198157637714E-5
96-97	0.0019085819627307433	5.84854450956749E-4	2.497599078818857E-5	2.497599078818857E-5	0.15565453725712253	4.995198157637714E-5
98-99	0.0019148259604277905	5.952611137851609E-4	2.497599078818857E-5	2.497599078818857E-5	0.1566889595422667	4.995198157637714E-5
100-101	0.0019252326232562023	5.994237789165256E-4	2.497599078818857E-5	2.497599078818857E-5	0.157525655233671	4.995198157637714E-5
102-103	0.0019273139558218847	6.035864440478904E-4	2.497599078818857E-5	2.497599078818857E-5	0.158495556209279	4.995198157637714E-5
104-105	0.0019418832837816615	6.077491091792552E-4	2.497599078818857E-5	2.9138655919553333E-5	0.15949459584080655	4.995198157637714E-5
106-107	0.0019606152768728027	6.1191177431062E-4	2.497599078818857E-5	2.9138655919553333E-5	0.1603749995160902	4.995198157637714E-5
108-109	0.001968940607135532	6.181557720076671E-4	2.497599078818857E-5	2.9138655919553333E-5	0.1612720538518993	4.995198157637714E-5
110-111	0.001981428602529627	6.285624348360791E-4	3.3301321050918095E-5	2.9138655919553333E-5	0.16219408417849657	4.995198157637714E-5
112-113	0.0019959979304894036	6.389690976644909E-4	4.162665131364762E-5	2.9138655919553333E-5	0.1630640811909518	4.995198157637714E-5
114-115	0.0020022419281864505	6.577010907556324E-4	4.578931644501238E-5	2.9138655919553333E-5	0.1638695568938709	5.8277311839106666E-5
116-117	0.00201056725844918	6.618637558869971E-4	4.578931644501238E-5	2.9138655919553333E-5	0.16483529520434753	6.243997697047143E-5
118-119	0.0020147299235805448	6.660264210183619E-4	4.578931644501238E-5	2.9138655919553333E-5	0.16568239755858025	6.868397466751856E-5
120-121	0.0020147299235805448	6.701890861497266E-4	4.578931644501238E-5	2.9138655919553333E-5	0.16664813586905686	7.076530723320095E-5
122-123	0.002016811256146227	6.764330838467738E-4	4.578931644501238E-5	2.9138655919553333E-5	0.1674244729160564	7.49279723645657E-5
124-125	0.002020973921277592	6.805957489781386E-4	4.995198157637714E-5	2.9138655919553333E-5	0.16839021122653303	7.909063749593048E-5
126-127	0.0020292992515403216	6.82677081543821E-4	4.995198157637714E-5	2.9138655919553333E-5	0.16948291082351627	8.741596775866E-5
128-129	0.0020397059143687332	6.910024118065505E-4	4.995198157637714E-5	2.9138655919553333E-5	0.17051108911096338	9.157863289002475E-5
130-131	0.0020417872469344154	6.9932774206928E-4	4.995198157637714E-5	2.9138655919553333E-5	0.17158505671485547	9.574129802138953E-5
132-133	0.0020584379074598744	7.034904072006447E-4	4.995198157637714E-5	2.9138655919553333E-5	0.17233641777106684	9.574129802138953E-5
134-135	0.002102145891339205	7.034904072006447E-4	4.995198157637714E-5	3.1219988485235715E-5	0.1731689507973398	9.782263058707191E-5
136-137	0.0021292032146930755	7.055717397663271E-4	4.995198157637714E-5	3.5382653616600474E-5	0.1741450957706448	9.990396315275428E-5
138-139	0.002135447212390123	7.180597351604214E-4	4.995198157637714E-5	3.746398618228285E-5	0.17501509278310004	9.990396315275428E-5
>>END_MODULE
