##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.4d.S.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23132287
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	62-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.22441434346721	37.0	35.0	38.0	33.0	39.0
2	35.559213103313134	35.0	34.0	38.0	33.0	39.0
3	35.66027699725496	36.0	35.0	38.0	33.0	39.0
4	35.765692644224934	36.0	35.0	38.0	33.0	39.0
5	35.70177215940646	36.0	35.0	38.0	33.0	39.0
6	35.625973860690905	36.0	35.0	37.0	33.0	39.0
7	35.591108652594535	35.0	34.0	38.0	33.0	39.0
8	35.74128009910996	36.0	35.0	38.0	33.0	39.0
9	35.73513358190654	36.0	35.0	38.0	33.0	39.0
10-14	35.65454712713879	36.0	35.0	37.8	33.0	39.0
15-19	35.661135753676234	36.0	35.0	38.0	33.0	39.0
20-24	35.64559853506919	36.0	35.0	38.0	33.0	39.0
25-29	35.63348090917253	36.0	35.0	38.0	33.0	39.0
30-34	35.608298885449585	36.0	35.0	38.0	33.0	39.0
35-39	35.585115107727994	36.0	35.0	38.0	33.0	39.0
40-44	35.56986520182808	36.0	35.0	38.0	33.0	39.0
45-49	35.53849043979093	36.0	35.0	38.0	33.0	39.0
50-54	35.52080604913816	36.0	34.8	38.0	33.0	39.0
55-59	35.488048086209545	36.0	34.4	38.0	33.0	39.0
60-64	35.450448534349036	36.0	34.0	38.0	33.0	39.0
65-69	35.41482169852116	36.0	34.0	38.0	32.8	39.0
70-74	35.38536528884659	36.0	34.0	38.0	32.0	39.0
75-79	35.33636881316239	36.0	34.0	38.0	32.0	39.0
80-84	35.29648276906696	36.0	34.0	38.0	32.0	39.0
85-89	35.25409312313521	36.0	34.0	38.0	32.0	39.0
90-94	35.20103412009307	36.0	34.0	38.0	32.0	39.0
95-99	35.13655053116228	36.0	34.0	38.0	32.0	39.0
100-104	35.0868707433159	36.0	34.0	38.0	32.0	39.0
105-109	35.02415284484399	36.0	34.0	38.0	31.6	39.0
110-114	34.95199393379876	35.8	34.0	37.6	31.0	39.0
115-119	34.88161862462489	35.0	34.0	37.0	31.0	39.0
120-124	34.80466206229276	35.0	34.0	37.0	31.0	39.0
125-129	34.7427148595425	35.0	34.0	37.0	30.8	39.0
130-134	34.6501365409459	35.0	34.0	37.0	30.0	39.0
135-139	34.56018696896295	35.0	34.0	37.0	30.0	39.0
140-144	34.469443648140704	35.0	34.0	37.0	30.0	39.0
145-149	34.43256179815119	35.0	34.0	37.0	30.0	39.0
150	35.140895484345634	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	12.0
21	135.0
22	1250.0
23	7292.0
24	25684.0
25	64732.0
26	125286.0
27	212963.0
28	309903.0
29	403891.0
30	517858.0
31	680388.0
32	922985.0
33	1323985.0
34	2075281.0
35	3754801.0
36	1.0543993E7
37	2161842.0
38	6.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.96042453562849	19.30373767193879	15.718722493802709	32.01711529863
2	24.61199035841075	25.85613025880797	31.992177513281657	17.539701869499623
3	20.315069595802164	29.53926818992295	29.874198339066016	20.271463875208877
4	23.726280230151055	34.00141689411933	21.891671233876323	20.380631641853295
5	25.73145059111535	35.083124292898496	21.792302680664474	17.39312243532168
6	19.37082655817069	39.382908373171595	21.876852119155018	19.3694129495027
7	20.622410599626857	21.243633249217133	36.851347073955424	21.282609077200583
8	21.65036254305508	23.980019787926718	28.003768066685318	26.36584960233288
9	23.784687773616493	23.690408289089977	27.752112350677315	24.772791586616215
10-14	23.164292089152944	29.3146430777519	25.166076186540515	22.354988646554645
15-19	23.312595075445845	28.06191968826947	26.66862640948558	21.9568588267991
20-24	23.181979369355048	28.112801816785343	26.611774270308853	22.093444543550753
25-29	23.326454491940204	27.718219128095722	26.570599785485978	22.3847265944781
30-34	23.163512740123902	27.43039049645537	26.789055719298794	22.617041044121933
35-39	23.466432207644942	27.154965068131524	26.796007042416576	22.582595681806954
40-44	23.62483225285939	26.831021939162348	26.874596532543453	22.669549275434804
45-49	23.549535967191968	26.650142518551174	26.975659838351994	22.824661675904864
50-54	23.67875815614354	26.64369116021587	26.771161481286505	22.90638920235408
55-59	23.790272012447364	26.56426405223141	26.821212273563788	22.82425166175744
60-64	23.519216643286793	26.50745992400077	26.903752783350004	23.069570649362436
65-69	23.67493092316528	26.468025557665843	26.859819111857203	22.997224407311677
70-74	23.86565295717006	26.361431000056918	26.679782652133916	23.09313339063911
75-79	23.724240916601552	26.493362483725765	26.86907403525064	22.91332256442205
80-84	23.987732372946418	26.48215483084979	26.579848817358414	22.950263978845378
85-89	23.810069287748288	26.58957068723651	26.738826893649374	22.86153313136583
90-94	23.789538973403427	26.392895256931347	26.726832623449955	23.09073314621527
95-99	23.78892019216436	26.600476590200305	26.786521781996132	22.824081435639204
100-104	24.020964618194984	26.33306301450289	26.74630290422442	22.8996694630777
105-109	23.828436865534094	26.541123839651892	26.701303068431866	22.929136226382145
110-114	23.92568630478729	26.630198431774936	26.601402623221727	22.842712640216043
115-119	24.019353267558614	26.65502762532213	26.74548288943762	22.58013621768163
120-124	23.932293539212804	26.75758596379968	26.923909132548346	22.38621136443917
125-129	23.950152114497612	26.860378974588645	26.761668021672087	22.427800889241652
130-134	24.13136098376033	26.849132635387534	26.702614466038916	22.316891914813223
135-139	23.960912754110943	26.879047229139307	26.918558926549302	22.241481090200445
140-144	23.948351625872533	27.012199214247534	26.778926463192864	22.260522696687065
145-149	23.73814898085644	27.2963072959932	26.58314885584297	22.38239486730739
150	24.125546171392592	26.0594338406715	26.769592513120077	23.045427474815835
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	9.5
3	27.5
4	39.5
5	46.5
6	44.5
7	35.0
8	25.0
9	23.0
10	18.5
11	13.5
12	16.0
13	14.0
14	25.5
15	46.5
16	68.5
17	110.0
18	211.5
19	367.0
20	590.0
21	916.0
22	1403.0
23	2210.5
24	3580.0
25	5661.5
26	8732.0
27	13378.0
28	19728.0
29	27687.0
30	38204.5
31	54696.0
32	80018.0
33	109127.5
34	144712.0
35	202947.0
36	286003.0
37	380280.5
38	491489.5
39	637313.0
40	811665.0
41	993818.0
42	1177319.0
43	1345344.5
44	1472841.0
45	1550106.0
46	1572214.5
47	1530141.5
48	1465513.0
49	1402595.5
50	1286485.5
51	1123151.0
52	959435.5
53	802392.0
54	658607.5
55	548416.5
56	451480.0
57	372587.5
58	314067.0
59	257029.5
60	196680.5
61	134366.5
62	82436.0
63	48779.0
64	30051.5
65	15491.0
66	8434.5
67	4461.0
68	2322.5
69	1379.0
70	876.0
71	676.0
72	434.5
73	321.0
74	261.0
75	160.0
76	111.5
77	48.5
78	12.0
79	3.0
80	2.5
81	1.5
82	0.0
83	0.0
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.322962100548035E-6
3	4.322962100548035E-6
4	4.322962100548035E-6
5	0.0
6	4.322962100548035E-6
7	4.322962100548035E-6
8	0.0
9	4.322962100548035E-6
10-14	0.09753467091256476
15-19	0.0
20-24	0.0
25-29	0.0
30-34	8.64592420109607E-7
35-39	8.64592420109607E-7
40-44	0.0
45-49	8.64592420109607E-7
50-54	1.729184840219214E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	8.645926219400684E-7
75-79	8.645931526798796E-7
80-84	0.0
85-89	0.0
90-94	0.0
95-99	4.3230330039879635E-6
100-104	8.646144575624615E-7
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62-63	1.0
64-65	1.0
66-67	1.0
68-69	0.0
70-71	1.0
72-73	6.0
74-75	4.0
76-77	11.0
78-79	5.0
80-81	27.0
82-83	23.0
84-85	20.0
86-87	32.0
88-89	38.0
90-91	58.0
92-93	48.0
94-95	65.0
96-97	76.0
98-99	89.0
100-101	83.0
102-103	79.0
104-105	99.0
106-107	111.0
108-109	126.0
110-111	139.0
112-113	148.0
114-115	147.0
116-117	153.0
118-119	181.0
120-121	235.0
122-123	266.0
124-125	280.0
126-127	346.0
128-129	432.0
130-131	465.0
132-133	575.0
134-135	809.0
136-137	1348.0
138-139	4348.0
140-141	8360.0
142-143	8479.0
144-145	56285.0
146-147	767941.0
148-149	843165.0
150-151	2.1437181E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.94399953528637
#Duplication Level	Percentage of total
1	22.13535035846121
2	9.500487840151491
3	6.089122721881399
4	4.302366940111814
5	3.256649419932927
6	2.578595229021694
7	2.1027056029715117
8	1.7505094519313364
9	1.5360533692545615
>10	19.07474818924559
>50	6.428015291904159
>100	12.643076122996616
>500	3.5457040618458397
>1k	4.266025178321798
>5k	0.4696506017246471
>10k+	0.3209396202436229
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.5448289224493883E-4	0.0	4.322962100548035E-6	0.0	0.0030390423566852686	0.0
2	0.0018934574000400394	8.64592420109607E-6	4.322962100548035E-6	0.0	0.013695143934536174	0.0
3	0.0027148201991441657	8.64592420109607E-6	4.322962100548035E-6	0.0	0.018087273428692978	0.0
4	0.00646715130241986	8.64592420109607E-6	4.322962100548035E-6	0.0	0.024524163996409002	0.0
5	0.006657361634843974	1.2968886301644105E-5	4.322962100548035E-6	0.0	0.02937020451112335	0.0
6	0.007867791022997424	1.2968886301644105E-5	4.322962100548035E-6	0.0	0.037052108163797205	0.0
7	0.010107085391081306	1.729184840219214E-5	4.322962100548035E-6	0.0	0.04755690606812893	0.0
8	0.017767374233252423	2.1614810502740176E-5	4.322962100548035E-6	0.0	0.06432999901825531	0.0
9	0.01835097411682641	2.593777260328821E-5	4.322962100548035E-6	0.0	0.07702221574546433	0.0
10-11	0.018990772507707518	2.593777260328821E-5	6.484443150822052E-6	0.0	0.1307501502121256	0.0
12-13	0.01973864495110233	3.242221575411026E-5	8.64592420109607E-6	0.0	0.1598480081109144	0.0
14-15	0.022276223704124024	3.458369680438428E-5	8.64592420109607E-6	0.0	0.1727196277652962	0.0
16-17	0.025001851308519563	3.458369680438428E-5	8.64592420109607E-6	0.0	0.18079708245017018	0.0
18-19	0.03125717746801257	3.8906658904932314E-5	8.64592420109607E-6	0.0	0.19033569832502942	0.0
20-21	0.03708020741745077	3.8906658904932314E-5	8.64592420109607E-6	0.0	0.19631651639113762	0.0
22-23	0.03956374914421562	5.187554520657642E-5	8.64592420109607E-6	0.0	0.2025415818159268	0.0
24-25	0.04462809924500764	5.187554520657642E-5	8.64592420109607E-6	0.0	0.20874070946811268	0.0
26-27	0.048773819899433205	5.187554520657642E-5	8.64592420109607E-6	0.0	0.21398662397712773	0.0
28-29	0.05874473198434725	5.187554520657642E-5	8.64592420109607E-6	0.0	0.2189969370516629	0.0
30-31	0.08117874380514127	5.6198507307124456E-5	1.2968886301644105E-5	4.322962100548035E-6	0.22398563531569532	0.0
32-33	0.10285407577728913	7.132887465904257E-5	1.2968886301644105E-5	4.322962100548035E-6	0.22818107003427718	0.0
34-35	0.11972227389362755	7.781331780986463E-5	1.2968886301644105E-5	4.322962100548035E-6	0.232391635120211	0.0
36-37	0.122754831807162	8.213627991041267E-5	1.2968886301644105E-5	4.322962100548035E-6	0.2350934864330535	4.322962100548035E-6
38-39	0.12535509351064164	8.64592420109607E-5	1.2968886301644105E-5	4.322962100548035E-6	0.23707772603720506	4.322962100548035E-6
40-41	0.1269308131962914	8.862072306123472E-5	1.2968886301644105E-5	4.322962100548035E-6	0.23884149457422865	4.322962100548035E-6
42-43	0.12723774350543032	9.294368516178275E-5	1.2968886301644105E-5	4.322962100548035E-6	0.24058364830074952	4.322962100548035E-6
44-45	0.1280936900013388	9.510516621205676E-5	1.2968886301644105E-5	4.322962100548035E-6	0.242023194680232	4.322962100548035E-6
46-47	0.12940354751780486	9.510516621205676E-5	1.2968886301644105E-5	4.322962100548035E-6	0.2434368032871112	4.322962100548035E-6
48-49	0.13055561691760093	9.726664726233078E-5	1.2968886301644105E-5	4.322962100548035E-6	0.2448006978298341	4.322962100548035E-6
50-51	0.13146776192081655	9.94281283126048E-5	1.2968886301644105E-5	4.322962100548035E-6	0.24635480270498114	8.64592420109607E-6
52-53	0.13229128620097097	9.94281283126048E-5	1.2968886301644105E-5	4.322962100548035E-6	0.2476560142972461	8.64592420109607E-6
54-55	0.13271709796787495	9.94281283126048E-5	1.2968886301644105E-5	4.322962100548035E-6	0.2488361829506957	8.64592420109607E-6
56-57	0.1336616651868447	9.94281283126048E-5	1.2968886301644105E-5	4.322962100548035E-6	0.25008768047880436	8.64592420109607E-6
58-59	0.13386268292452017	1.0375109041315284E-4	1.2968886301644105E-5	4.322962100548035E-6	0.25115112915553917	8.64592420109607E-6
60-61	0.1342020354494132	1.0591257146342686E-4	1.2968886301644105E-5	4.322962100548035E-6	0.25211947266606194	8.64592420109607E-6
62-63	0.1344830279859488	1.0807405251370088E-4	1.2968886301644105E-5	4.322962100548035E-6	0.25320885911540003	8.64592420109607E-6
64-65	0.13451977316380348	1.102355335639749E-4	1.2968886301644105E-5	4.322962100548035E-6	0.25436308999624635	8.64592420109607E-6
66-67	0.1346019094437139	1.1455849566452292E-4	1.2968886301644105E-5	4.322962100548035E-6	0.2551952602006019	8.64592420109607E-6
68-69	0.13464513906471937	1.1888145776507096E-4	1.2968886301644105E-5	4.322962100548035E-6	0.25604472225335956	8.64592420109607E-6
70-71	0.13473592126883088	1.2104293881534497E-4	1.2968886301644105E-5	4.322962100548035E-6	0.2569719976239271	1.2968886301644105E-5
72-73	0.13478779681403746	1.2536590091589302E-4	1.2968886301644105E-5	4.322962100548035E-6	0.2576485411926629	1.2968886301644105E-5
74-75	0.134815896067691	1.2752738196616703E-4	1.2968886301644105E-5	4.322962100548035E-6	0.2584720654728173	1.2968886301644105E-5
76-77	0.13485047976449538	1.361733061672631E-4	1.2968886301644105E-5	4.322962100548035E-6	0.25918751570045795	1.2968886301644105E-5
78-79	0.1349174856770539	1.4698071141863318E-4	1.2968886301644105E-5	4.322962100548035E-6	0.26009317626052275	1.5130367351918122E-5
80-81	0.1349585538170091	1.5778811667000326E-4	1.2968886301644105E-5	4.322962100548035E-6	0.2610312590363417	3.0260734703836243E-5
82-83	0.13499097603276322	1.6427255982082533E-4	1.2968886301644105E-5	4.322962100548035E-6	0.2618893670133005	3.0260734703836243E-5
84-85	0.13502339824851733	1.6427255982082533E-4	1.2968886301644105E-5	4.322962100548035E-6	0.2627734127628626	3.0260734703836243E-5
86-87	0.13503852861586924	1.6427255982082533E-4	1.5130367351918122E-5	4.322962100548035E-6	0.2636012600051175	3.0260734703836243E-5
88-89	0.1350687893505731	1.6427255982082533E-4	1.729184840219214E-5	4.322962100548035E-6	0.26445072205787523	3.0260734703836243E-5
90-91	0.13509040416107582	1.6427255982082533E-4	1.729184840219214E-5	8.64592420109607E-6	0.26511213525925903	3.242221575411026E-5
92-93	0.13510985749052828	1.6427255982082533E-4	1.729184840219214E-5	8.64592420109607E-6	0.26569573514283307	3.458369680438428E-5
94-95	0.13515524859258404	1.6427255982082533E-4	1.9453329452466157E-5	1.2968886301644105E-5	0.26645873795357977	3.8906658904932314E-5
96-97	0.13516173303573487	1.6427255982082533E-4	2.1614810502740176E-5	1.2968886301644105E-5	0.267154734851768	3.8906658904932314E-5
98-99	0.13517686340308677	1.6427255982082533E-4	2.1614810502740176E-5	1.2968886301644105E-5	0.2678312784205038	3.8906658904932314E-5
100-101	0.13519415525148898	1.6427255982082533E-4	2.1614810502740176E-5	1.2968886301644105E-5	0.2684516234819324	3.8906658904932314E-5
102-103	0.13521144709989116	1.6427255982082533E-4	2.1614810502740176E-5	1.2968886301644105E-5	0.269087098910713	3.8906658904932314E-5
104-105	0.13522873894829335	1.6427255982082533E-4	2.1614810502740176E-5	1.2968886301644105E-5	0.26973986618789575	4.106813995520633E-5
106-107	0.13524386931564528	1.6427255982082533E-4	2.1614810502740176E-5	1.2968886301644105E-5	0.2702521371968107	4.322962100548035E-5
108-109	0.1352762915313994	1.6859552192137337E-4	2.593777260328821E-5	1.2968886301644105E-5	0.27085086744773657	4.5391102055754363E-5
110-111	0.13530655226610322	1.6859552192137337E-4	2.593777260328821E-5	1.2968886301644105E-5	0.27148201991441656	4.755258310602838E-5
112-113	0.13531735967135458	1.707570029716474E-4	2.593777260328821E-5	1.2968886301644105E-5	0.27210452645689553	4.97140641563024E-5
114-115	0.13533032855765625	1.729184840219214E-4	2.593777260328821E-5	1.2968886301644105E-5	0.2726967722646706	5.187554520657642E-5
116-117	0.1353411359629076	1.729184840219214E-4	2.593777260328821E-5	1.2968886301644105E-5	0.27327821066719427	5.187554520657642E-5
118-119	0.1353497818871087	1.729184840219214E-4	3.0260734703836243E-5	1.9453329452466157E-5	0.27384668018341635	5.8359988357398475E-5
120-121	0.13536275077341034	1.729184840219214E-4	3.0260734703836243E-5	2.1614810502740176E-5	0.27443892599119146	6.26829504579465E-5
122-123	0.1353843655839131	1.729184840219214E-4	3.0260734703836243E-5	2.1614810502740176E-5	0.2750614325336703	6.916739360876855E-5
124-125	0.135399495951265	1.729184840219214E-4	3.0260734703836243E-5	2.1614810502740176E-5	0.27575310646975804	7.349035570931659E-5
126-127	0.1354081418754661	1.7724144612246944E-4	3.0260734703836243E-5	2.1614810502740176E-5	0.276509624837354	7.349035570931659E-5
128-129	0.1354167877996672	1.858873703235655E-4	3.0260734703836243E-5	2.1614810502740176E-5	0.2772574972807488	7.781331780986463E-5
130-131	0.13542975668596885	1.9237181347438756E-4	3.0260734703836243E-5	2.1614810502740176E-5	0.27797727047049003	8.213627991041267E-5
132-133	0.13544921001542132	1.9669477557493557E-4	3.0260734703836243E-5	2.1614810502740176E-5	0.2786494910771252	8.213627991041267E-5
134-135	0.13546001742067268	2.0317921872575764E-4	3.0260734703836243E-5	2.1614810502740176E-5	0.2793173887216599	8.213627991041267E-5
136-137	0.13546866334487376	2.0750218082630568E-4	3.0260734703836243E-5	2.1614810502740176E-5	0.27996367155569185	8.64592420109607E-5
138-139	0.13546866334487376	2.1830958607767578E-4	3.0260734703836243E-5	2.1614810502740176E-5	0.28051052626141115	9.078220411150873E-5
>>END_MODULE
