##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.4d.S.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24035536
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	56-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.17986122714301	37.0	35.0	38.0	33.0	39.0
2	35.52629444169666	35.0	34.0	38.0	33.0	39.0
3	35.61506092479069	36.0	35.0	38.0	33.0	39.0
4	35.720641886247094	36.0	35.0	38.0	33.0	39.0
5	35.652406253806866	36.0	35.0	38.0	33.0	39.0
6	35.57836155598943	36.0	35.0	37.0	33.0	39.0
7	35.54559107814363	35.0	34.0	38.0	33.0	39.0
8	35.69516681466975	36.0	35.0	38.0	33.0	39.0
9	35.6880180662499	36.0	35.0	38.0	33.0	39.0
10-14	35.59863248316992	36.0	35.0	37.8	33.0	39.0
15-19	35.607106644095644	36.0	35.0	38.0	33.0	39.0
20-24	35.59376712880462	36.0	35.0	38.0	33.0	39.0
25-29	35.57280797066477	36.0	35.0	38.0	33.0	39.0
30-34	35.547589319414385	36.0	35.0	38.0	33.0	39.0
35-39	35.52282087655544	36.0	35.0	38.0	33.0	39.0
40-44	35.503608423793835	36.0	35.0	38.0	33.0	39.0
45-49	35.46386384726348	36.0	34.0	38.0	33.0	39.0
50-54	35.44748840217252	36.0	34.0	38.0	33.0	39.0
55-59	35.40880627089927	36.0	34.0	38.0	32.4	39.0
60-64	35.37047617063295	36.0	34.0	38.0	32.0	39.0
65-69	35.32911657779709	36.0	34.0	38.0	32.0	39.0
70-74	35.29526674281684	36.0	34.0	38.0	32.0	39.0
75-79	35.243089520581044	36.0	34.0	38.0	32.0	39.0
80-84	35.200015049982724	36.0	34.0	38.0	32.0	39.0
85-89	35.15274740019547	36.0	34.0	38.0	32.0	39.0
90-94	35.09481167701095	36.0	34.0	38.0	32.0	39.0
95-99	35.02566369817238	35.8	34.0	38.0	31.4	39.0
100-104	34.969199899478795	35.8	34.0	38.0	31.0	39.0
105-109	34.9002162619106	35.0	34.0	37.0	31.0	39.0
110-114	34.825004402526005	35.0	34.0	37.0	31.0	39.0
115-119	34.744728481310005	35.0	34.0	37.0	30.8	39.0
120-124	34.66253511125758	35.0	34.0	37.0	30.0	39.0
125-129	34.595458402002464	35.0	34.0	37.0	30.0	39.0
130-134	34.496084669431	35.0	34.0	37.0	30.0	39.0
135-139	34.39438019897251	35.0	34.0	37.0	30.0	39.0
140-144	34.29303934464203	35.0	34.0	37.0	29.0	39.0
145-149	34.253436458251755	35.0	34.0	37.0	29.0	39.0
150	35.020503781180494	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	146.0
22	1285.0
23	7089.0
24	27081.0
25	69067.0
26	140364.0
27	241655.0
28	353675.0
29	457966.0
30	583695.0
31	763371.0
32	1027809.0
33	1461932.0
34	2262954.0
35	4004629.0
36	1.0525537E7
37	2107271.0
38	2.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.98961171492078	19.041476753420437	16.23182857249366	31.73708295916513
2	24.963110867434857	25.090430693156225	32.09020860530912	17.856249834099795
3	20.493918691637194	28.994582396439274	30.21832882022389	20.293170091699643
4	23.91837534869728	33.49603688838521	22.03466426144991	20.550923501467597
5	26.198652378599117	34.726663447543956	21.79481429453575	17.279869879321176
6	19.502773705682024	39.15970249882101	21.897586219736738	19.439937575760226
7	20.847606643762802	20.990848716666854	37.148008681811795	21.013535957758545
8	21.718078812731136	23.619413807049757	28.25364846288202	26.408858917337096
9	23.913876845839997	23.17356460646974	28.05893557430428	24.85362297338599
10-14	23.26262657715228	29.042459023650224	25.34670347990237	22.348210919295123
15-19	23.434844972876828	27.887668492185902	26.727091087130322	21.950395447806947
20-24	23.276543918580288	27.998158692997883	26.644294125902483	22.08100326251935
25-29	23.445337132863084	27.61548257226702	26.607276214745333	22.331904080124563
30-34	23.27606193825727	27.359028127011836	26.831178858097264	22.53373107663362
35-39	23.585594263427286	27.104276767532877	26.811948774514537	22.498180194525304
40-44	23.753731974190213	26.83864175111385	26.862949925477007	22.54467634921892
45-49	23.66481612891845	26.676523460928852	26.9825569939443	22.676103416208402
50-54	23.79377660961112	26.652540471388875	26.802877875564196	22.75080504343581
55-59	23.90274859448315	26.59995832670322	26.824435123671098	22.672857955142533
60-64	23.64678562997602	26.538894455184565	26.924705729442085	22.889614185397335
65-69	23.784243297232912	26.502310417254566	26.892242344942645	22.82120394056988
70-74	23.95734991892124	26.44035525724592	26.69774350860469	22.904551315228144
75-79	23.826083403805487	26.527769884360207	26.910735113539147	22.735411598295155
80-84	24.095765023139148	26.551651859652676	26.575984245940663	22.776598871267513
85-89	23.912235738389867	26.667682723698977	26.76765028737076	22.6524312505404
90-94	23.88856616892347	26.457738842799706	26.757949957408456	22.895745030868365
95-99	23.877447296364014	26.657942878883418	26.82748308024222	22.63712674451035
100-104	24.14004181654323	26.39796273497341	26.766553568858498	22.695441879624862
105-109	23.913444693738086	26.601893314111024	26.763696050377423	22.720965941773468
110-114	23.998925831951617	26.692694879213967	26.648725919977746	22.659653368856674
115-119	24.096056416889045	26.700407970991613	26.804229395977018	22.399306216142325
120-124	24.001610307030187	26.794048002168736	26.982688034369552	22.221653656431528
125-129	24.03617964749067	26.8787366488781	26.813773414135934	22.271310289495304
130-134	24.1937380202712	26.871148446631842	26.771642455320364	22.163471077776588
135-139	24.036362172955833	26.910155428550407	26.98477817910051	22.06870421939325
140-144	24.025639718517496	27.039866210754028	26.846679354016356	22.08781471671212
145-149	23.7838962156281	27.340467176434412	26.65053156946274	22.225105038474748
150	24.128703112063718	26.078529810962607	26.87564816631136	22.91711891066231
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	12.5
3	31.0
4	47.0
5	53.0
6	48.0
7	41.0
8	31.0
9	31.5
10	29.5
11	19.5
12	21.5
13	23.5
14	27.5
15	46.5
16	72.0
17	114.0
18	203.5
19	373.0
20	603.0
21	933.5
22	1408.0
23	2200.0
24	3650.5
25	5852.0
26	9056.0
27	13628.0
28	19946.0
29	28208.5
30	39227.5
31	55454.5
32	80779.5
33	110140.0
34	145438.5
35	204783.5
36	289798.5
37	386754.5
38	502083.0
39	656626.0
40	842673.5
41	1032052.5
42	1226438.0
43	1407976.5
44	1543182.5
45	1624790.0
46	1646012.0
47	1602816.5
48	1536775.5
49	1466915.5
50	1342285.5
51	1174193.0
52	1001336.0
53	830830.5
54	678807.5
55	561352.0
56	458732.5
57	379337.0
58	321241.5
59	262693.0
60	200281.5
61	136659.5
62	84554.0
63	50546.0
64	30773.0
65	15468.0
66	8414.5
67	4402.0
68	2148.5
69	1258.0
70	839.0
71	657.0
72	418.0
73	317.5
74	267.5
75	172.0
76	114.5
77	40.5
78	14.0
79	2.5
80	3.5
81	2.5
82	1.5
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	8.321012687214466E-6
3	4.160506343607233E-6
4	1.2481519030821697E-5
5	8.321012687214466E-6
6	4.160506343607233E-6
7	0.0
8	1.2481519030821697E-5
9	8.321012687214466E-6
10-14	0.10960937172360126
15-19	0.0
20-24	1.6642025374428929E-6
25-29	8.321012687214464E-7
30-34	8.321012687214464E-7
35-39	0.0
40-44	0.0
45-49	0.0
50-54	2.4963038061643394E-6
55-59	0.0
60-64	0.0
65-69	8.321015179828288E-7
70-74	0.0
75-79	3.3284102262941176E-6
80-84	0.0
85-89	0.0
90-94	0.0
95-99	9.153269483786723E-6
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	1.6970497977201494E-6
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
56-57	1.0
58-59	0.0
60-61	1.0
62-63	1.0
64-65	4.0
66-67	0.0
68-69	5.0
70-71	6.0
72-73	5.0
74-75	9.0
76-77	10.0
78-79	19.0
80-81	17.0
82-83	23.0
84-85	24.0
86-87	28.0
88-89	47.0
90-91	50.0
92-93	51.0
94-95	70.0
96-97	67.0
98-99	100.0
100-101	103.0
102-103	123.0
104-105	144.0
106-107	110.0
108-109	126.0
110-111	155.0
112-113	184.0
114-115	191.0
116-117	185.0
118-119	235.0
120-121	245.0
122-123	312.0
124-125	320.0
126-127	385.0
128-129	468.0
130-131	551.0
132-133	711.0
134-135	933.0
136-137	1559.0
138-139	4513.0
140-141	8882.0
142-143	9741.0
144-145	60962.0
146-147	801836.0
148-149	956360.0
150-151	2.2185664E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.06134875888604
#Duplication Level	Percentage of total
1	22.451979415783796
2	9.18575120367119
3	5.927532778381426
4	4.3314871859202135
5	3.1918354826050694
6	2.4893218637412633
7	2.083901029188021
8	1.7990950933062018
9	1.5497529880678977
>10	19.361920814135296
>50	6.667253782039226
>100	12.866611023429392
>500	3.476926883509193
>1k	3.9090237543290662
>5k	0.510058480292474
>10k+	0.1975482216001438
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.496303806164339E-4	0.0	0.0	0.0	0.003016367099115243	0.0
2	0.0013729670933903867	0.0	0.0	0.0	0.010688340796726979	0.0
3	0.0020386481083675438	0.0	4.160506343607232E-6	0.0	0.014212289669762306	4.160506343607232E-6
4	0.0052214354612270765	0.0	4.160506343607232E-6	0.0	0.01970415804332385	4.160506343607232E-6
5	0.005412818753033009	0.0	4.160506343607232E-6	0.0	0.023806417298120584	4.160506343607232E-6
6	0.006432142807216781	4.160506343607232E-6	4.160506343607232E-6	0.0	0.0306046846635748	4.160506343607232E-6
7	0.008183715977875426	8.321012687214464E-6	4.160506343607232E-6	0.0	0.039811885201977606	4.160506343607232E-6
8	0.014407833467911845	8.321012687214464E-6	4.160506343607232E-6	0.0	0.05480635006433807	4.160506343607232E-6
9	0.01498614384967325	8.321012687214464E-6	4.160506343607232E-6	0.0	0.06602723567304677	4.160506343607232E-6
10-11	0.015551972712403835	1.2481519030821697E-5	4.160506343607232E-6	2.080253171803616E-6	0.1111458467162954	4.160506343607232E-6
12-13	0.01614276461319606	1.2481519030821697E-5	4.160506343607232E-6	4.160506343607232E-6	0.13429074350578246	4.160506343607232E-6
14-15	0.018639068419360402	1.2481519030821697E-5	4.160506343607232E-6	4.160506343607232E-6	0.14452350885788443	8.321012687214464E-6
16-17	0.021420366910061835	1.4561772202625313E-5	4.160506343607232E-6	4.160506343607232E-6	0.15151107926197277	8.321012687214464E-6
18-19	0.02686022895432829	1.8722278546232545E-5	4.160506343607232E-6	4.160506343607232E-6	0.16024606233037614	8.321012687214464E-6
20-21	0.03205878163066553	2.4963038061643394E-5	4.160506343607232E-6	4.160506343607232E-6	0.16595427703380528	8.321012687214464E-6
22-23	0.03420560290396686	2.9123544405250625E-5	4.160506343607232E-6	4.160506343607232E-6	0.17134629325512024	8.321012687214464E-6
24-25	0.03846596139982067	2.9123544405250625E-5	4.160506343607232E-6	4.160506343607232E-6	0.17740815099775598	1.2481519030821697E-5
26-27	0.042249941919331443	2.9123544405250625E-5	4.160506343607232E-6	1.040126585901808E-5	0.18220313455876333	1.2481519030821697E-5
28-29	0.052478546765089826	2.9123544405250625E-5	4.160506343607232E-6	1.2481519030821697E-5	0.18635948039602696	1.6642025374428928E-5
30-31	0.07384274683951296	2.9123544405250625E-5	4.160506343607232E-6	1.6642025374428928E-5	0.19074049357584538	1.8722278546232545E-5
32-33	0.09192430740883	4.576556977967955E-5	4.160506343607232E-6	1.6642025374428928E-5	0.19436221434795548	2.0802531718036163E-5
34-35	0.10562277454515681	4.992607612328679E-5	4.160506343607232E-6	1.6642025374428928E-5	0.19781335435997766	2.0802531718036163E-5
36-37	0.10801506569273096	5.20063292950904E-5	4.160506343607232E-6	1.6642025374428928E-5	0.20004338576015113	2.4963038061643394E-5
38-39	0.10996426291471095	5.8247088810501244E-5	4.160506343607232E-6	1.6642025374428928E-5	0.20183448374107404	2.4963038061643394E-5
40-41	0.11127066190660362	6.240759515410848E-5	4.160506343607232E-6	1.6642025374428928E-5	0.20350492703803236	2.4963038061643394E-5
42-43	0.11149532924915842	6.240759515410848E-5	4.160506343607232E-6	1.6642025374428928E-5	0.20516496906913162	2.4963038061643394E-5
44-45	0.11214020773241754	6.656810149771571E-5	4.160506343607232E-6	1.6642025374428928E-5	0.20651713363080398	2.4963038061643394E-5
46-47	0.11325730368567608	7.696936735673379E-5	4.160506343607232E-6	1.6642025374428928E-5	0.20789634148370978	2.9123544405250625E-5
48-49	0.11416845457492605	8.112987370034104E-5	4.160506343607232E-6	1.6642025374428928E-5	0.20936291996983133	2.9123544405250625E-5
50-51	0.11506712394514523	8.529038004394826E-5	6.2407595154108485E-6	1.6642025374428928E-5	0.21091894934234046	2.9123544405250625E-5
52-53	0.11590962647972569	9.569164590296634E-5	8.321012687214464E-6	1.6642025374428928E-5	0.21229399668890264	3.3284050748857856E-5
54-55	0.11632567711408641	1.0401265859018081E-4	8.321012687214464E-6	1.6642025374428928E-5	0.2134755804904871	3.3284050748857856E-5
56-57	0.11710161154716917	1.1025341810559164E-4	8.321012687214464E-6	1.6642025374428928E-5	0.21461555922863546	3.3284050748857856E-5
58-59	0.11724306876285182	1.1441392444919889E-4	1.2481519030821697E-5	1.6642025374428928E-5	0.2156806488525989	3.3284050748857856E-5
60-61	0.11755094623227874	1.2273493713641336E-4	1.2481519030821697E-5	1.6642025374428928E-5	0.21649194758960233	3.3284050748857856E-5
62-63	0.11784218167633125	1.289756966518242E-4	1.2481519030821697E-5	1.6642025374428928E-5	0.21739269721299329	3.5364303920661474E-5
64-65	0.1178733854739083	1.3729670933903865E-4	1.2481519030821697E-5	1.6642025374428928E-5	0.2183745767100846	3.744455709246509E-5
66-67	0.11796075610712406	1.5393873471346758E-4	1.2481519030821697E-5	1.6642025374428928E-5	0.21916923342171357	3.744455709246509E-5
68-69	0.11804188598082438	1.5809924105707483E-4	1.2481519030821697E-5	1.6642025374428928E-5	0.2199888531714042	3.744455709246509E-5
70-71	0.11812925661404014	1.6225974740068205E-4	1.2481519030821697E-5	1.6642025374428928E-5	0.2208022321615794	4.576556977967955E-5
72-73	0.11817502218381983	1.6225974740068205E-4	1.4561772202625313E-5	1.6642025374428928E-5	0.221484555201931	4.992607612328679E-5
74-75	0.11819790496870966	1.6225974740068205E-4	1.6642025374428928E-5	1.6642025374428928E-5	0.22225632912867016	4.992607612328679E-5
76-77	0.1182166272472559	1.7266101325970015E-4	2.4963038061643394E-5	1.6642025374428928E-5	0.22304266482761193	4.992607612328679E-5
78-79	0.11827071382972279	1.78901772775111E-4	2.4963038061643394E-5	1.6642025374428928E-5	0.22405366786910846	5.20063292950904E-5
80-81	0.11831855965267427	1.8722278546232546E-4	2.9123544405250625E-5	1.8722278546232545E-5	0.22489617040368892	5.4086582466894016E-5
82-83	0.11834768319707953	1.9762405132134353E-4	3.120379757705424E-5	2.0802531718036163E-5	0.22569290736848974	5.4086582466894016E-5
84-85	0.11836432522245396	2.0386481083675437E-4	3.3284050748857856E-5	2.0802531718036163E-5	0.22650420610549316	5.4086582466894016E-5
86-87	0.11838096724782837	2.0386481083675437E-4	3.3284050748857856E-5	2.0802531718036163E-5	0.22725517750051422	5.4086582466894016E-5
88-89	0.11841009079223364	2.0386481083675437E-4	3.3284050748857856E-5	2.0802531718036163E-5	0.22813928509853076	5.824708881050125E-5
90-91	0.11842049205809264	2.1218582352396884E-4	3.3284050748857856E-5	2.0802531718036163E-5	0.22898802839262666	6.032734198230487E-5
92-93	0.11843713408346708	2.1218582352396884E-4	3.5364303920661474E-5	2.0802531718036163E-5	0.22963290687588578	6.240759515410848E-5
94-95	0.1184537761088415	2.1218582352396884E-4	3.744455709246509E-5	2.0802531718036163E-5	0.23035475472650163	6.656810149771571E-5
96-97	0.11845793661518511	2.1218582352396884E-4	3.744455709246509E-5	2.0802531718036163E-5	0.2310807630834611	6.656810149771571E-5
98-99	0.11846001686835692	2.1634632986757606E-4	3.744455709246509E-5	2.0802531718036163E-5	0.2317630861238127	6.656810149771571E-5
100-101	0.11847249838738774	2.1634632986757606E-4	3.744455709246509E-5	2.0802531718036163E-5	0.23238508182218195	6.656810149771571E-5
102-103	0.11849122066593398	2.225870893829869E-4	3.952481026426871E-5	2.0802531718036163E-5	0.23305908384984633	6.656810149771571E-5
104-105	0.11852034421033922	2.309081020702014E-4	4.1605063436072326E-5	2.0802531718036163E-5	0.2338620615741625	6.656810149771571E-5
106-107	0.11852866522302644	2.32988355242005E-4	4.1605063436072326E-5	2.0802531718036163E-5	0.2344736560066728	7.280886101312657E-5
108-109	0.11854530724840087	2.3922911475741585E-4	4.576556977967955E-5	2.0802531718036163E-5	0.23503116385671613	8.112987370034104E-5
110-111	0.11857235053963432	2.4546987427282666E-4	4.992607612328679E-5	2.0802531718036163E-5	0.2356281965170238	8.321012687214465E-5
112-113	0.11858483205866513	2.5379088696004116E-4	5.408658246689402E-5	2.0802531718036163E-5	0.23625851322808028	8.737063321575187E-5
114-115	0.11859731357769596	2.5379088696004116E-4	5.824708881050125E-5	2.0802531718036163E-5	0.23686802740741875	8.737063321575187E-5
116-117	0.11860355433721137	2.579513933036484E-4	5.824708881050125E-5	2.0802531718036163E-5	0.2374629798145546	8.737063321575187E-5
118-119	0.11861187534989859	2.641921528190593E-4	5.824708881050125E-5	2.0802531718036163E-5	0.23813282133587535	8.737063321575187E-5
120-121	0.1186160358562422	2.6627240599086285E-4	5.824708881050125E-5	2.4963038061643394E-5	0.2387881010849935	8.737063321575187E-5
122-123	0.1186264371221012	2.683526591626665E-4	5.824708881050125E-5	2.4963038061643394E-5	0.23941009678336278	9.15311395593591E-5
124-125	0.11864099889430384	2.704329123344701E-4	5.824708881050125E-5	2.4963038061643394E-5	0.2401714694442429	9.15311395593591E-5
126-127	0.11865348041333465	2.704329123344701E-4	5.824708881050125E-5	2.9123544405250625E-5	0.24087043450996892	9.15311395593591E-5
128-129	0.11865556066650645	2.704329123344701E-4	5.824708881050125E-5	2.9123544405250625E-5	0.24164012818353625	9.15311395593591E-5
130-131	0.11866388167919367	2.7459341867807735E-4	5.824708881050125E-5	2.9123544405250625E-5	0.2424805504649449	9.361139273116273E-5
132-133	0.1186763631982245	2.7459341867807735E-4	6.240759515410848E-5	2.9123544405250625E-5	0.2431711945179837	9.569164590296634E-5
134-135	0.11868468421091172	2.8083417819348817E-4	6.240759515410848E-5	2.9123544405250625E-5	0.243782788950494	9.985215224657358E-5
136-137	0.11869092497042712	2.829144313652918E-4	6.240759515410848E-5	2.9123544405250625E-5	0.2444193464210659	9.985215224657358E-5
138-139	0.11869092497042712	2.8707493770889904E-4	6.240759515410848E-5	2.9123544405250625E-5	0.24508502743604305	1.019324054183772E-4
>>END_MODULE
