##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.2d.R.1.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24169843
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	63-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.44293212827241	36.0	34.0	38.0	31.0	39.0
2	34.76031470291305	35.0	34.0	37.0	31.0	39.0
3	34.86342894324965	35.0	34.0	38.0	31.0	39.0
4	34.881797080767136	36.0	34.0	38.0	31.0	39.0
5	34.75469571730358	35.0	34.0	37.0	31.0	39.0
6	34.74491290655053	35.0	34.0	37.0	32.0	39.0
7	34.759651148747636	35.0	34.0	37.0	32.0	39.0
8	34.89705543391407	35.0	34.0	37.0	32.0	39.0
9	34.86796467813217	35.0	34.0	37.0	32.0	39.0
10-14	34.846386366680164	35.0	34.0	37.0	31.6	39.0
15-19	34.85764912912343	35.0	34.0	37.0	31.0	39.0
20-24	34.8525232125008	35.0	34.0	37.0	31.0	39.0
25-29	34.84513881203118	35.0	34.0	37.0	31.0	39.0
30-34	34.835463432675176	35.0	34.0	37.0	31.0	39.0
35-39	34.82289027694553	35.0	34.0	37.0	31.0	39.0
40-44	34.78369208273302	35.0	34.0	37.0	31.0	39.0
45-49	34.68865049723327	35.0	34.0	37.0	31.0	39.0
50-54	34.66760268984784	35.0	34.0	37.0	31.0	39.0
55-59	34.65076262183416	35.0	34.0	37.0	31.0	39.0
60-64	34.63058853643904	35.0	34.0	37.0	31.0	39.0
65-69	34.592625710999684	35.0	34.0	37.0	31.0	39.0
70-74	34.59807583653923	35.0	34.0	37.0	31.0	39.0
75-79	34.57868536495005	35.0	34.0	37.0	30.8	39.0
80-84	34.569763389308044	35.0	34.0	37.0	30.8	39.0
85-89	34.546914733073514	35.0	34.0	37.0	30.0	39.0
90-94	34.52786209777124	35.0	34.0	37.0	30.0	39.0
95-99	34.511671352735085	35.0	34.0	37.0	30.0	39.0
100-104	34.47493970143358	35.0	34.0	37.0	30.0	39.0
105-109	34.449343302992816	35.0	34.0	37.0	30.0	39.0
110-114	34.41719105739911	35.0	34.0	37.0	30.0	39.0
115-119	34.38361506510881	35.0	34.0	37.0	30.0	39.0
120-124	34.34365735111254	35.0	34.0	37.0	30.0	39.0
125-129	34.33514284023698	35.0	34.0	37.0	30.0	39.0
130-134	34.28447290968803	35.0	34.0	37.0	30.0	39.0
135-139	34.25426122279234	35.0	34.0	37.0	30.0	39.0
140-144	34.20956907624939	35.0	34.0	37.0	29.8	39.0
145-149	34.24782102745114	35.0	34.0	37.0	30.0	39.0
150	35.088086956339566	35.0	34.0	37.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	4.0
22	193.0
23	2469.0
24	17082.0
25	71353.0
26	188724.0
27	358151.0
28	540168.0
29	711895.0
30	895332.0
31	1128123.0
32	1447565.0
33	1916455.0
34	2680580.0
35	4285097.0
36	8908081.0
37	1018558.0
38	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.34822890657585	10.934617986554567	15.047135390991162	39.67001771587842
2	22.372154944636062	13.004082470614847	36.953820968824544	27.669941615924543
3	20.789239263110733	17.907196761347386	28.76999095194962	32.53357302359226
4	25.899288641962087	25.65668449578412	22.50441107850756	25.939615783746227
5	25.55324771913937	31.06835614524955	24.675016251669852	18.70337988394123
6	18.861493073741393	36.29047716552093	26.268615614234125	18.57941414650355
7	15.875343910212674	24.46230270751639	40.78738092694586	18.874972455325082
8	18.331662102847616	24.182397582174396	31.893527861362287	25.592412453615697
9	19.533268368019773	23.033095694006175	32.88265560593105	24.550980332043004
10-14	20.244344960685353	29.282488872910083	27.131201426825413	23.34196473957915
15-19	20.427906546146765	27.971459458650767	27.914841632272193	23.68579236293028
20-24	20.576455065191176	28.137533353145972	27.630537813802775	23.655473767860077
25-29	20.55188056476741	28.32692818231611	27.51414579284008	23.607045460076407
30-34	20.53311563779772	28.042555752450642	27.53182873327365	23.892499876477984
35-39	20.64163219556351	28.037583713526647	27.56444055728101	23.75634353362883
40-44	20.70011223355887	28.102906472092197	27.452825980242217	23.744155314106717
45-49	20.79494099122235	27.891321705100175	27.414751652413756	23.89898565126372
50-54	20.91773604018221	27.856757997034748	27.48908957588813	23.73641638689491
55-59	20.919039061223422	27.888247610359407	27.423030806097238	23.769682522319936
60-64	20.868107753669836	27.78721301367416	27.41117847993571	23.933500752720292
65-69	20.97981217493506	27.7150091005702	27.48984648204253	23.815332242452218
70-74	21.09110499580249	27.662560663620923	27.450734322626918	23.795600017949674
75-79	21.110968876702515	27.559358776761282	27.4510575261595	23.878614820376697
80-84	21.161322728792996	27.554426995588237	27.43331303444644	23.850937241172332
85-89	21.138747864750833	27.568748788052623	27.48059241650983	23.811910930686714
90-94	21.144116607863474	27.432685582573317	27.4447370263506	23.978460783212608
95-99	21.231508031302127	27.346016543024852	27.558588052320882	23.863887373352142
100-104	21.285999072832695	27.417876130842117	27.413342323605256	23.88278247271993
105-109	21.254499759207967	27.22782941229615	27.532305264419342	23.985365564076538
110-114	21.346476704307154	27.224670588502818	27.556667191521488	23.872185515668537
115-119	21.377709940209215	27.273238596944672	27.502825502623114	23.846225960223002
120-124	21.34450278964707	27.156233724314177	27.508131543447373	23.991131942591384
125-129	21.387031702637213	27.091190156282796	27.579941784388073	23.94183635669192
130-134	21.461666459257238	27.19626764982101	27.468323499191417	23.873742391730335
135-139	21.446052442749334	27.0498410141771	27.572187476907956	23.931919066165612
140-144	21.52033120395728	26.990350812452164	27.675173284397438	23.814144699193122
145-149	21.135241407814114	27.06423500543016	27.822471508003595	23.978052078752132
150	21.145853799432544	25.670898657478542	28.44358087942811	24.7396666636608
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	3.5
11	6.0
12	9.0
13	16.0
14	39.0
15	82.5
16	157.5
17	316.5
18	638.5
19	1243.0
20	2279.5
21	3881.5
22	6079.5
23	9651.0
24	15434.0
25	23766.0
26	34894.5
27	48851.5
28	66765.5
29	93211.5
30	126328.0
31	161469.5
32	194027.0
33	228843.5
34	269370.5
35	318466.0
36	393136.5
37	493549.0
38	612733.0
39	759732.0
40	927395.5
41	1092386.5
42	1260683.0
43	1432254.5
44	1572569.5
45	1655263.0
46	1676329.5
47	1635593.0
48	1545486.5
49	1417353.5
50	1252858.0
51	1073406.0
52	890918.0
53	711717.0
54	551134.0
55	417569.0
56	315095.5
57	237801.5
58	178850.0
59	132660.0
60	93271.0
61	65328.5
62	46445.5
63	31844.0
64	22620.5
65	17469.5
66	15323.5
67	11347.5
68	6708.5
69	4787.0
70	3900.0
71	3115.0
72	2541.0
73	1657.0
74	834.0
75	261.5
76	57.5
77	30.0
78	14.5
79	1.0
80	0.5
81	0.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0016963287680437148
3	0.14630628755015082
4	7.033558306522719E-5
5	7.861035754348922E-5
6	4.964864686957214E-5
7	7.033558306522719E-5
8	1.1170945545653731E-4
9	9.102251926088225E-5
10-14	1.9197476789567893E-4
15-19	2.90444584186997E-4
20-24	3.47540528087005E-4
25-29	3.1940629486091406E-4
30-34	2.581729637217751E-4
35-39	1.381887337869758E-4
40-44	2.763774675739516E-4
45-49	2.0273197471741956E-4
50-54	9.019504181305604E-5
55-59	1.5225585040002122E-4
60-64	1.7128783311739205E-4
65-69	0.05925814492291674
70-74	1.762527095130439E-4
75-79	1.2825904686564887E-4
80-84	9.929737919213985E-5
85-89	7.86105045529177E-5
90-94	1.3818925635958955E-4
95-99	9.102314516675706E-5
100-104	8.026628015722062E-5
105-109	9.598903368319735E-5
110-114	5.2959886692322416E-5
115-119	5.5442951197509E-5
120-124	9.930180733757936E-5
125-129	9.682042389934543E-5
130-134	5.710031539814357E-5
135-139	4.468925586620759E-5
140-144	3.477049243883769E-5
145-149	4.7244440185695616E-5
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62-63	1.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	3.0
74-75	1.0
76-77	4.0
78-79	8.0
80-81	3.0
82-83	6.0
84-85	13.0
86-87	15.0
88-89	14.0
90-91	22.0
92-93	27.0
94-95	26.0
96-97	39.0
98-99	48.0
100-101	65.0
102-103	51.0
104-105	37.0
106-107	64.0
108-109	69.0
110-111	79.0
112-113	121.0
114-115	98.0
116-117	88.0
118-119	90.0
120-121	108.0
122-123	102.0
124-125	114.0
126-127	138.0
128-129	171.0
130-131	203.0
132-133	284.0
134-135	390.0
136-137	706.0
138-139	2263.0
140-141	5176.0
142-143	8631.0
144-145	73561.0
146-147	768668.0
148-149	1351294.0
150-151	2.1957042E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.118568020381396
#Duplication Level	Percentage of total
1	39.964852659567605
2	10.150683828395547
3	6.19435339826885
4	4.464873264112882
5	3.3991396614401808
6	2.6003878983515274
7	2.129886610403029
8	1.865601726716186
9	1.510991680266054
>10	17.882373989657786
>50	4.447903687489128
>100	4.6123594524049
>500	0.42955627050779166
>1k	0.32431794998463237
>5k	0.02271792243376197
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	4.551125963044113E-5	0.0	0.0	1.9031981300002653E-4	1.6549548956524044E-5
2	0.0	1.1170945545653731E-4	0.0	4.137387239131011E-6	0.0019983580365002785	4.551125963044113E-5
3	0.0	1.1170945545653731E-4	0.0	8.274774478262022E-6	0.0030533917824786864	9.102251926088225E-5
4	2.482432343478607E-5	1.2412161717393033E-4	0.0	8.274774478262022E-6	0.00458422506095716	9.929729373914428E-5
5	2.896171067391708E-5	1.2825900441306134E-4	0.0	8.274774478262022E-6	0.005846128168892119	1.1584684269566832E-4
6	4.964864686957214E-5	1.2825900441306134E-4	0.0	1.2412161717393035E-5	0.006859788042479217	1.2412161717393033E-4
7	6.206080858696516E-5	1.6963287680437148E-4	0.0	1.2412161717393035E-5	0.00802239385667503	1.3239639165219235E-4
8	8.274774478262023E-5	1.6963287680437148E-4	0.0	1.2412161717393035E-5	0.01026485774028404	1.4480855336958538E-4
9	9.515990650001327E-5	1.6963287680437148E-4	0.0	1.2412161717393035E-5	0.013893346349001936	1.5722071508697842E-4
10-11	1.468772469891509E-4	1.71701570423937E-4	0.0	1.2412161717393035E-5	0.01860996780161129	2.0893805557611607E-4
12-13	2.0893805557611607E-4	1.8411373214133E-4	0.0	1.2412161717393035E-5	0.022610821261850975	2.6272408968481924E-4
14-15	2.1100674919568159E-4	1.9445720023915754E-4	0.0	1.2412161717393035E-5	0.02693232223312332	3.1857881741308787E-4
16-17	2.4410584710872967E-4	2.172128300543781E-4	0.0	1.2412161717393035E-5	0.031559989860091356	3.268535918913499E-4
18-19	2.8341102588047426E-4	2.2755629815220563E-4	0.0	1.2412161717393035E-5	0.035618766741678874	3.309909791304809E-4
20-21	3.55815302565267E-4	2.4203715348916416E-4	0.0	1.2412161717393035E-5	0.03935069003137505	3.3926575360874295E-4
22-23	4.6131867716310776E-4	2.503119279674262E-4	0.0	1.2412161717393035E-5	0.043500489432223455	3.6409007704352896E-4
24-25	5.378603410870315E-4	2.585867024456882E-4	0.0	2.0686936195655058E-5	0.048020584990974086	3.7236485152179104E-4
26-27	6.164706986305207E-4	2.606553960652537E-4	0.0	2.0686936195655058E-5	0.052300712089855116	3.9718917495657705E-4
28-29	6.516384901631343E-4	2.606553960652537E-4	0.0	2.482432343478607E-5	0.05653739662272527	4.2408219201092864E-4
30-31	6.868062816957479E-4	2.606553960652537E-4	0.0	3.309909791304809E-5	0.06059203611707366	4.571812899239767E-4
32-33	7.24042766847927E-4	2.606553960652537E-4	0.0	3.309909791304809E-5	0.06449152358995464	5.316542602283349E-4
34-35	7.654166392392372E-4	2.606553960652537E-4	0.0	3.309909791304809E-5	0.06793796716015077	5.502725028044245E-4
36-37	7.798974945761956E-4	2.606553960652537E-4	0.0	3.9305178771744604E-5	0.07140096027930343	5.564785836631211E-4
38-39	8.026531243914162E-4	2.647927833043847E-4	0.0	4.551125963044113E-5	0.07567901868456489	5.626846645218175E-4
40-41	8.564391585001194E-4	2.6893017054351574E-4	0.0	4.551125963044113E-5	0.07915856135267407	5.709594390000796E-4
42-43	8.709200138370778E-4	2.772049450217777E-4	0.0	4.551125963044113E-5	0.08260086753563108	5.792342134783415E-4
44-45	9.12293886228388E-4	2.854797195000398E-4	0.0	4.757995325000663E-5	0.08681065905144689	5.813029070979071E-4
46-47	9.784920820544842E-4	2.854797195000398E-4	0.0	4.964864686957214E-5	0.09079496296273004	5.895776815761692E-4
48-49	0.0010053850991088359	2.9375449397830175E-4	0.0	4.964864686957214E-5	0.09444620720126316	6.123333113913896E-4
50-51	0.0010281407289240564	2.978918812174328E-4	0.0	5.378603410870315E-5	0.09846361021045938	6.206080858696517E-4
52-53	0.0010798580694131939	2.978918812174328E-4	0.0	6.206080858696516E-5	0.10225552561512294	6.247454731087827E-4
54-55	0.001133644103521897	2.978918812174328E-4	0.0	6.619819582609618E-5	0.10541442077219948	6.247454731087827E-4
56-57	0.0011646745078153797	3.061666556956948E-4	0.0	6.619819582609618E-5	0.10950836544531961	6.309515539674791E-4
58-59	0.0011936362184892966	3.496092217065705E-4	0.0	7.861035754348921E-5	0.11338095990114624	6.350889412066103E-4
60-61	0.0012225979291632138	3.64090077043529E-4	0.0	8.274774478262023E-5	0.11681085392238584	6.412950220653068E-4
62-63	0.0012598344143153928	3.76502238760922E-4	0.0	8.274774478262023E-5	0.12032349568840808	6.495697965435687E-4
64-65	0.0012784526568914824	3.76502238760922E-4	0.0	8.274774478262023E-5	0.12518699438800657	6.495697965435687E-4
66-67	0.0013032769803262686	3.9718917495657705E-4	0.0	8.274774478262023E-5	0.12912371834603975	6.495697965435687E-4
68-69	0.0013632690952936682	4.1167003029353563E-4	0.0	8.274774478262023E-5	0.13183577568129012	6.578445710218308E-4
70-71	0.0014625663890328125	4.1787611115223213E-4	0.0	8.274774478262023E-5	0.134421642705747	6.578445710218308E-4
72-73	0.0014956654869458606	4.1787611115223213E-4	0.0	8.481643840218574E-5	0.1372102417049213	6.619819582609618E-4
74-75	0.0015142837295219502	4.2201349839136317E-4	8.274774478262022E-6	9.722860011957877E-5	0.14031948821512824	6.661193455000928E-4
76-77	0.0015535889082936949	4.302882728696252E-4	8.274774478262022E-6	1.1170945545653731E-4	0.1428164014139438	6.661193455000928E-4
78-79	0.0015887566998263082	4.323569664891907E-4	8.274774478262022E-6	1.3239639165219235E-4	0.14501128534430283	6.702567327392238E-4
80-81	0.0016321992658371838	4.385630473478872E-4	8.274774478262022E-6	1.3239639165219235E-4	0.14760749583685753	6.806002008370514E-4
82-83	0.0016673670573697976	4.4890651544571476E-4	8.274774478262022E-6	1.3446508527175787E-4	0.1502885227678144	6.826688944566168E-4
84-85	0.0017439087212937213	4.5304390268484574E-4	8.274774478262022E-6	1.3653377889132336E-4	0.15254753620037997	6.868062816957479E-4
86-87	0.0018556181767502584	4.5511259630441126E-4	8.274774478262022E-6	1.3653377889132336E-4	0.15458519941565196	6.888749753153134E-4
88-89	0.001975602406685058	4.654560644022388E-4	8.274774478262022E-6	1.3860247251088885E-4	0.15672009123104358	6.909436689348789E-4
90-91	0.0020190449726959336	4.7373083888050077E-4	8.274774478262022E-6	1.4067116613045437E-4	0.1593390573534135	6.99218443413141E-4
92-93	0.0020645562323263747	4.757995325000663E-4	8.274774478262022E-6	1.4067116613045437E-4	0.1620262903652291	7.033558306522719E-4
94-95	0.0021245483472937744	4.778682261196318E-4	8.274774478262022E-6	1.4067116613045437E-4	0.16499279701568603	7.033558306522719E-4
96-97	0.002180403075022043	4.840743069783283E-4	8.274774478262022E-6	1.4067116613045437E-4	0.16727663477168636	7.033558306522719E-4
98-99	0.0022569447389459664	4.902803878370249E-4	8.274774478262022E-6	1.427398597500199E-4	0.16933291622953445	7.033558306522719E-4
100-101	0.0023169368539133666	5.006238559348524E-4	8.274774478262022E-6	1.4687724698915088E-4	0.17150297583645868	7.054245242718374E-4
102-103	0.002345898564587283	5.047612431739834E-4	8.274774478262022E-6	1.530833278478474E-4	0.17332756360891544	7.07493217891403E-4
104-105	0.0023789976625003314	5.088986304131144E-4	1.0343468097827527E-5	1.5928940870654394E-4	0.17503837323229612	7.157679923696649E-4
106-107	0.0024658827945220826	5.192420985109419E-4	1.2412161717393035E-5	1.6342679594567497E-4	0.17670987767690505	7.219740732283615E-4
108-109	0.0025465618456851375	5.275168729892039E-4	1.2412161717393035E-5	1.6549548956524047E-4	0.1784351681556227	7.24042766847927E-4
110-111	0.002625172203228627	5.378603410870315E-4	1.2412161717393035E-5	1.6549548956524047E-4	0.17999082575753594	7.24042766847927E-4
112-113	0.0026913703990547226	5.399290347065969E-4	1.2412161717393035E-5	1.6549548956524047E-4	0.181666467589384	7.24042766847927E-4
114-115	0.0027472251267829915	5.461351155652936E-4	1.2412161717393035E-5	1.6756418318480596E-4	0.18363586391521036	7.261114604674925E-4
116-117	0.0028175607098482186	5.60615970902252E-4	1.2412161717393035E-5	1.737702640435025E-4	0.18535287961944974	7.32317541326189E-4
118-119	0.0029313388589243213	5.916463751957346E-4	1.2412161717393035E-5	1.820450385217645E-4	0.1868671633489717	7.32317541326189E-4
120-121	0.003072010025054776	6.040585369131277E-4	1.2412161717393035E-5	1.9031981300002653E-4	0.1883173175762871	7.343862349457545E-4
122-123	0.0032540550635765404	6.040585369131277E-4	1.2412161717393035E-5	1.9031981300002653E-4	0.18981298306323297	7.385236221848855E-4
124-125	0.0033533523573156844	6.040585369131277E-4	1.2412161717393035E-5	1.9031981300002653E-4	0.19130037377570058	7.40592315804451E-4
126-127	0.003398863616946126	6.123333113913896E-4	1.2412161717393035E-5	1.9238850661959202E-4	0.19254158994743986	7.40592315804451E-4
128-129	0.003487817442587442	6.123333113913896E-4	1.2412161717393035E-5	1.9445720023915754E-4	0.19388210341291834	7.40592315804451E-4
130-131	0.00359952689804398	6.164706986305207E-4	1.2412161717393035E-5	1.9859458747828855E-4	0.19511090742294024	7.40592315804451E-4
132-133	0.0036926181109244276	6.185393922500861E-4	1.2412161717393035E-5	2.0066328109785405E-4	0.1963479862074404	7.40592315804451E-4
134-135	0.003851907519630972	6.206080858696517E-4	1.2412161717393035E-5	2.0273197471741956E-4	0.19752714157059276	7.447297030435821E-4
136-137	0.004042227332630998	6.475011029240033E-4	1.2412161717393035E-5	2.0273197471741956E-4	0.1985614883803755	7.447297030435821E-4
138-139	0.004062914268826653	6.557758774022654E-4	1.2412161717393035E-5	2.0686936195655058E-4	0.1996703081604626	7.447297030435821E-4
>>END_MODULE
