##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.2d.R.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24201870
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	68-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.86775472308545	36.0	35.0	38.0	33.0	39.0
2	35.2199022637507	35.0	34.0	37.0	32.0	39.0
3	35.339002936549946	35.0	34.0	37.0	33.0	39.0
4	35.40346477358981	36.0	35.0	37.0	33.0	39.0
5	35.31813186336428	35.0	34.0	37.0	33.0	39.0
6	35.276660605151584	35.0	34.0	37.0	33.0	39.0
7	35.3148171608227	35.0	34.0	37.0	33.0	39.0
8	35.458537542760126	36.0	34.0	38.0	33.0	39.0
9	35.431252171836306	36.0	34.0	38.0	33.0	39.0
10-14	35.39181869004337	35.4	34.2	37.6	33.0	39.0
15-19	35.3630474504656	35.8	34.0	38.0	33.0	39.0
20-24	35.333906594820974	35.8	34.0	37.8	32.8	39.0
25-29	35.3218738552021	35.8	34.0	37.8	32.2	39.0
30-34	35.304777283738815	36.0	34.0	37.8	32.0	39.0
35-39	35.27794842299376	35.4	34.0	37.4	32.0	39.0
40-44	35.25881114971695	35.6	34.0	37.6	32.0	39.0
45-49	35.2303938332038	35.2	34.0	37.0	32.0	39.0
50-54	35.20783294844572	35.2	34.0	37.2	32.0	39.0
55-59	35.17815926620546	35.0	34.0	37.0	32.0	39.0
60-64	35.13899687916678	35.0	34.0	37.0	32.0	39.0
65-69	35.10326793069714	35.0	34.0	37.0	32.0	39.0
70-74	35.08379996028181	35.0	34.0	37.0	32.0	39.0
75-79	35.03189664614003	35.0	34.0	37.0	32.0	39.0
80-84	34.96237575824301	35.0	34.0	37.0	31.6	39.0
85-89	34.94262199314806	35.0	34.0	37.0	31.2	39.0
90-94	34.892696710455	35.0	34.0	37.0	31.0	39.0
95-99	34.829285177776384	35.0	34.0	37.0	31.0	39.0
100-104	34.767502030202806	35.0	34.0	37.0	31.0	39.0
105-109	34.70969872682568	35.0	34.0	37.0	30.8	39.0
110-114	34.64667549380956	35.0	34.0	37.0	30.0	39.0
115-119	34.58755960647219	35.0	34.0	37.0	30.0	39.0
120-124	34.5140100082523	35.0	34.0	37.0	30.0	39.0
125-129	34.43517067092904	35.0	34.0	37.0	30.0	39.0
130-134	34.36101209574824	35.0	34.0	37.0	30.0	39.0
135-139	34.273191418155065	35.0	34.0	37.0	29.0	39.0
140-144	34.178147481795165	35.0	34.0	37.0	29.0	39.0
145-149	34.15005783608473	35.0	34.0	37.0	29.0	39.0
150	34.973402245870155	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	9.0
21	179.0
22	1702.0
23	9182.0
24	32524.0
25	80373.0
26	163069.0
27	283181.0
28	414980.0
29	544213.0
30	691796.0
31	890569.0
32	1179825.0
33	1629724.0
34	2421638.0
35	4076891.0
36	1.0192033E7
37	1589970.0
38	12.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.06144112004568	19.000102058229384	16.425891883561064	31.512564938163866
2	24.265319167485817	25.54373277767379	33.39070080121908	16.80024725362131
3	19.664303612254077	29.497664002726403	30.337177678302446	20.50085470671707
4	23.388940606655602	34.07534211199382	22.46493762672058	20.070779654629995
5	25.143123237997727	35.20068077384103	22.513322317655618	17.142873670505626
6	18.805621218525676	38.80579062692263	23.018171736316244	19.37041641823545
7	20.201170405427348	20.512733106987188	38.22207126969941	21.064025217886055
8	21.46806424462242	23.17325066203562	28.4529707828362	26.905714310505758
9	23.443168789980643	23.25711291140366	28.676024979723675	24.623693318892023
10-14	23.114947729245717	29.1157303134014	25.66629686053185	22.103025096821032
15-19	22.94432245019616	28.251957857007877	27.13944545837897	21.664274234416997
20-24	23.059116830606065	28.323379107107137	27.00151762197354	21.615986440313254
25-29	23.166453666596837	28.297612539857457	26.920963545378935	21.614970248166774
30-34	23.169422858646875	28.070149124840356	27.082341984317743	21.678086032195033
35-39	23.25896469983518	28.049925894156114	27.06362359602791	21.627485809980797
40-44	23.425067567092956	27.803863089918256	27.15635031507896	21.614719027909825
45-49	23.439601981169222	27.62819401971831	27.225225984603668	21.706978014508795
50-54	23.52372377443333	27.580209525794576	27.194065807262547	21.702000892509552
55-59	23.678546327205293	27.470356629467062	27.09741354696972	21.753683496357926
60-64	23.611921723404016	27.430816709617893	27.15538262126026	21.80187894571783
65-69	23.68121676021261	27.35909129929367	27.11526964016036	21.84442230033336
70-74	23.751745083147632	27.3152297240814	27.021615871649246	21.91140932112172
75-79	23.667808160623323	27.356367611535397	27.119593924979362	21.85623030286192
80-84	23.780375424159068	27.408046348363552	26.991763683603843	21.819814543873534
85-89	23.826159597119233	27.311334215165484	27.042250981425504	21.820255206289776
90-94	23.752389596995066	27.245998873815413	27.106633985529704	21.894977543659817
95-99	23.83570393128324	27.384384653798644	27.083943705159257	21.695967709758854
100-104	23.9817066024707	27.352797279507012	26.886850204866125	21.77864591315616
105-109	23.75929360752362	27.499518912334032	26.975783966287125	21.765403513855222
110-114	23.791405328987004	27.506549568080324	26.91168552962439	21.790359573308276
115-119	23.87742562681651	27.498906336267652	26.930855757282917	21.692812279632914
120-124	23.84401150765075	27.40309420684031	27.12184377499926	21.63105051050968
125-129	23.829534171233867	27.486264850930276	27.06822265540314	21.61597832243272
130-134	23.873467010691492	27.39918227345186	27.14422194200227	21.583128773854373
135-139	23.798459527186978	27.404370947313588	27.231762852240106	21.56540667325933
140-144	23.82374830671194	27.51438347839165	27.218943461034804	21.442924753861604
145-149	23.52143956741857	27.461222167392503	27.32861623287303	21.688722032315898
150	23.646269194806823	26.30162675139435	27.7962941773782	22.25580987642063
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	7.0
3	19.5
4	33.0
5	32.5
6	24.5
7	18.5
8	11.0
9	10.5
10	10.0
11	5.0
12	5.5
13	14.5
14	29.0
15	46.0
16	88.0
17	155.0
18	250.0
19	403.5
20	628.0
21	978.5
22	1514.0
23	2491.0
24	4106.5
25	6636.0
26	10334.5
27	16704.5
28	28052.0
29	43579.5
30	58786.0
31	76055.0
32	99822.5
33	131458.5
34	177623.5
35	246780.0
36	344542.5
37	467439.0
38	614399.0
39	798045.5
40	1009404.0
41	1206656.5
42	1392844.0
43	1566253.0
44	1686150.0
45	1744445.5
46	1748151.0
47	1695583.0
48	1594163.5
49	1450188.5
50	1263540.5
51	1062689.5
52	870220.0
53	694869.0
54	545787.5
55	417358.0
56	313073.5
57	233302.0
58	169978.0
59	121679.0
60	84907.0
61	57639.5
62	39090.0
63	27430.5
64	19173.0
65	13813.5
66	10519.5
67	7899.5
68	7014.0
69	5515.0
70	3270.0
71	2383.5
72	1831.5
73	1533.0
74	1313.0
75	780.5
76	265.0
77	92.5
78	24.5
79	1.5
80	0.5
81	1.0
82	1.0
83	0.5
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	4.131912120840249E-6
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	4.131912120840249E-6
10-14	0.0
15-19	1.6527648483360998E-6
20-24	0.026381432509140823
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.263824241680499E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	5.842536485235497E-4
80-84	0.05123104454863845
85-89	8.263922445005085E-7
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	3.250427606984992E-4
145-149	0.026755775624374496
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
68-69	5.0
70-71	10.0
72-73	9.0
74-75	16.0
76-77	23.0
78-79	27.0
80-81	43.0
82-83	62.0
84-85	62.0
86-87	56.0
88-89	93.0
90-91	104.0
92-93	98.0
94-95	100.0
96-97	125.0
98-99	162.0
100-101	143.0
102-103	140.0
104-105	132.0
106-107	175.0
108-109	181.0
110-111	188.0
112-113	198.0
114-115	212.0
116-117	219.0
118-119	278.0
120-121	276.0
122-123	318.0
124-125	347.0
126-127	428.0
128-129	475.0
130-131	575.0
132-133	748.0
134-135	1143.0
136-137	1634.0
138-139	3419.0
140-141	7255.0
142-143	11537.0
144-145	73180.0
146-147	841063.0
148-149	1119178.0
150-151	2.2137433E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.78642284685335
#Duplication Level	Percentage of total
1	28.35911272139919
2	10.72877193736149
3	7.001400205778401
4	5.17793586648441
5	3.9134986420878537
6	3.194726025437653
7	2.487038347162097
8	2.219025564424074
9	1.9047488218972193
>10	21.21760452342336
>50	5.276930973745843
>100	6.411928136838259
>500	0.9949293127205272
>1k	0.975459341462508
>5k	0.08236330562472814
>10k+	0.05452627415248082
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	8.263824241680498E-6	8.263824241680498E-6	0.0	0.0	0.001194122602922832	0.0
2	2.8923384845881743E-5	3.718720908756224E-5	0.0	0.0	0.005516102681321733	0.0
3	3.718720908756224E-5	4.131912120840249E-5	0.0	0.0	0.007255637684195478	0.0
4	1.3635309998772822E-4	4.131912120840249E-5	0.0	0.0	0.008767917520423009	0.0
5	1.4048501210856847E-4	4.545103332924274E-5	0.0	0.0	0.010486792962692552	0.0
6	1.4874883635024896E-4	4.958294545008299E-5	0.0	0.0	0.01211063442618277	0.0
7	1.9006795755865147E-4	6.197868181260374E-5	0.0	0.0	0.01441624138961163	0.0
8	2.933657605796577E-4	6.197868181260374E-5	0.0	0.0	0.020535603240576038	0.0
9	3.098934090630187E-4	6.197868181260374E-5	0.0	4.131912120840249E-6	0.02704336483089943	0.0
10-11	3.5121253027142117E-4	6.197868181260374E-5	0.0	4.131912120840249E-6	0.03356972002576661	0.0
12-13	3.966635636006639E-4	6.404463787302386E-5	0.0	4.131912120840249E-6	0.03992666682367933	0.0
14-15	4.441805529903268E-4	6.817654999386411E-5	0.0	4.131912120840249E-6	0.048653265222893934	0.0
16-17	4.875656302591494E-4	7.024250605428423E-5	0.0	4.131912120840249E-6	0.054632142061749774	0.0
18-19	5.743357847967946E-4	7.024250605428423E-5	0.0	4.131912120840249E-6	0.06260879841103187	0.0
20-21	6.776335878178009E-4	7.850633029596473E-5	0.0	6.197868181260374E-6	0.0687508857786609	0.0
22-23	7.499420499325051E-4	8.677015453764524E-5	0.0	8.263824241680498E-6	0.07712420569154367	0.0
24-25	8.222505120472096E-4	8.883611059806536E-5	0.0	8.263824241680498E-6	0.08290061883647834	0.0
26-27	8.842291938598133E-4	9.090206665848549E-5	0.0	8.263824241680498E-6	0.08749323915879229	0.0
28-29	0.0010846269317205653	9.296802271890561E-5	0.0	8.263824241680498E-6	0.09210238712958957	2.0659560604201246E-6
30-31	0.0014317075498711464	1.012318469605861E-4	0.0	8.263824241680498E-6	0.09810398948511004	4.131912120840249E-6
32-33	0.0016341712437923186	1.1156162726268672E-4	0.0	1.0329780302100622E-5	0.1048761934511672	4.131912120840249E-6
34-35	0.0018097775089280293	1.1156162726268672E-4	0.0	1.2395736362520748E-5	0.10838005492963973	4.131912120840249E-6
36-37	0.0018841519271031535	1.1982545150436723E-4	0.0	1.2395736362520748E-5	0.11178888242933294	4.131912120840249E-6
38-39	0.001941998696794917	1.2808927574604773E-4	0.0	1.2395736362520748E-5	0.11548694377748497	4.131912120840249E-6
40-41	0.0019853837740637396	1.3635309998772822E-4	0.0	1.2395736362520748E-5	0.11839374395449609	4.131912120840249E-6
42-43	0.0019998454664866805	1.4048501210856847E-4	0.0	1.2395736362520748E-5	0.12123236758151333	4.131912120840249E-6
44-45	0.0020205050270908818	1.5081479241066907E-4	0.0	1.2395736362520748E-5	0.12356276601766723	4.131912120840249E-6
46-47	0.0020638901043597046	2.024636939211722E-4	0.0	1.2395736362520748E-5	0.12647783001892002	4.131912120840249E-6
48-49	0.0021114070937493673	2.127934742232728E-4	0.0	1.2395736362520748E-5	0.12883921779598023	4.131912120840249E-6
50-51	0.0021713198195015505	2.1485943028369295E-4	0.0	1.2395736362520748E-5	0.13103119717608597	4.131912120840249E-6
52-53	0.002239496369495415	2.1485943028369295E-4	0.0	1.2395736362520748E-5	0.13306409793953938	4.131912120840249E-6
54-55	0.0022994090952475985	2.189913424045332E-4	0.0	1.2395736362520748E-5	0.13515277951662413	6.197868181260374E-6
56-57	0.0023965090300873447	2.272551666462137E-4	0.0	1.2395736362520748E-5	0.13722906535734636	8.263824241680498E-6
58-59	0.002464685580081208	2.437828151295747E-4	0.0	1.2395736362520748E-5	0.1392764278132227	8.263824241680498E-6
60-61	0.002549389778558434	2.5617855149209545E-4	0.0	1.2395736362520748E-5	0.14068954175855006	8.263824241680498E-6
62-63	0.002594840811887676	2.6857428785461617E-4	0.0	1.2395736362520748E-5	0.14264806810382835	8.263824241680498E-6
64-65	0.002619632284612718	2.7890406815671683E-4	0.0	1.2395736362520748E-5	0.14440206479912504	8.263824241680498E-6
66-67	0.002687808834606582	2.9749767270049793E-4	0.0	1.2395736362520748E-5	0.1457552660187002	8.263824241680498E-6
68-69	0.0027084683952107833	3.160912772442791E-4	0.0	1.2395736362520748E-5	0.14698037796252933	1.2395736362520748E-5
70-71	0.002737391780056665	3.305529696672199E-4	0.0	1.6527648483360997E-5	0.1482695345442315	1.6527648483360997E-5
72-73	0.0027869747255067478	3.5534444239226145E-4	0.0	1.6527648483360997E-5	0.1491826871229372	2.0659560604201244E-5
74-75	0.002805568330050529	3.6360826663394194E-4	0.0	1.859360454378112E-5	0.15019500559254306	2.0659560604201244E-5
76-77	0.002834491714896411	3.7600400299646266E-4	0.0	2.0659560604201244E-5	0.1515461408560578	2.0659560604201244E-5
78-79	0.0028778767921652333	3.883997393589834E-4	0.0	2.0659560604201244E-5	0.15337244601346922	2.0659560604201244E-5
80-81	0.0028882065724673343	3.945976075402438E-4	0.0	2.4791472725041495E-5	0.15494257261938849	2.0659560604201244E-5
82-83	0.002915064001252796	4.111252560236048E-4	4.131912120840249E-6	2.4791472725041495E-5	0.15680606498588745	2.0659560604201244E-5
84-85	0.002935723561856997	4.131912120840249E-4	4.131912120840249E-6	2.8923384845881743E-5	0.1582666959206045	2.4791472725041495E-5
86-87	0.0029522512103403583	4.214550363257054E-4	4.131912120840249E-6	2.8923384845881743E-5	0.16014464997952638	2.4791472725041495E-5
88-89	0.0029791086391258194	4.338507726882262E-4	4.131912120840249E-6	2.8923384845881743E-5	0.1618821190263397	2.4791472725041495E-5
90-91	0.0030039001118508614	4.875656302591494E-4	4.131912120840249E-6	2.8923384845881743E-5	0.1628283269020121	2.4791472725041495E-5
92-93	0.0030121639360925416	4.916975423799897E-4	8.263824241680498E-6	2.8923384845881743E-5	0.1637704028655637	2.8923384845881743E-5
94-95	0.003014229892152962	5.020273226820902E-4	8.263824241680498E-6	2.8923384845881743E-5	0.16478478729122997	2.8923384845881743E-5
96-97	0.003018361804273802	5.102911469237708E-4	8.263824241680498E-6	2.8923384845881743E-5	0.16569793986993567	2.8923384845881743E-5
98-99	0.0030224937163946426	5.164890151050311E-4	8.263824241680498E-6	2.8923384845881743E-5	0.16654084994258708	2.8923384845881743E-5
100-101	0.0030266256285154825	5.206209272258713E-4	8.263824241680498E-6	3.3055296966721993E-5	0.16724534095919036	2.8923384845881743E-5
102-103	0.0030286915845759025	5.226868832862915E-4	1.0329780302100622E-5	3.3055296966721993E-5	0.16810891059244595	2.8923384845881743E-5
104-105	0.003039021364878003	5.309507075279721E-4	1.2395736362520748E-5	3.3055296966721993E-5	0.1689022377196473	2.8923384845881743E-5
106-107	0.0030782745300259855	5.743357847967946E-4	1.2395736362520748E-5	3.3055296966721993E-5	0.16966250954988188	2.8923384845881743E-5
108-109	0.003086538354267666	5.867315211593154E-4	1.6527648483360997E-5	3.3055296966721993E-5	0.1704537707210228	3.3055296966721993E-5
110-111	0.0030927362224489265	6.032591696426763E-4	1.859360454378112E-5	3.3055296966721993E-5	0.17140204455275565	3.3055296966721993E-5
112-113	0.0031154617391135476	6.177208620656172E-4	2.0659560604201244E-5	3.3055296966721993E-5	0.17217471211935276	3.512125302714212E-5
114-115	0.003119593651234388	6.30116598428138E-4	2.0659560604201244E-5	3.3055296966721993E-5	0.17300316049958123	3.718720908756224E-5
116-117	0.0031237255633552283	6.363144666093984E-4	2.2725516664621368E-5	3.3055296966721993E-5	0.17382541101162843	4.338507726882261E-5
118-119	0.0031278574754760686	6.363144666093984E-4	2.8923384845881743E-5	3.3055296966721993E-5	0.17455056158883592	4.545103332924274E-5
120-121	0.003131989387596909	6.404463787302386E-4	2.8923384845881743E-5	3.3055296966721993E-5	0.1753707461448227	4.545103332924274E-5
122-123	0.003131989387596909	6.487102029719192E-4	2.8923384845881743E-5	3.3055296966721993E-5	0.1760669733371843	4.545103332924274E-5
124-125	0.003140253211838589	6.507761590323392E-4	2.8923384845881743E-5	3.3055296966721993E-5	0.17690161958559403	5.371485757092324E-5
126-127	0.00315471490426153	6.528421150927594E-4	2.8923384845881743E-5	3.3055296966721993E-5	0.17787675084611232	5.578081363134336E-5
128-129	0.0031609127724427904	6.569740272135996E-4	2.8923384845881743E-5	3.3055296966721993E-5	0.17882709063390556	5.9912725752183616E-5
130-131	0.0031629787285032103	6.569740272135996E-4	3.098934090630187E-5	3.718720908756224E-5	0.17971751769594663	6.197868181260374E-5
132-133	0.003191902113349092	6.569740272135996E-4	3.3055296966721993E-5	3.718720908756224E-5	0.18047778952618124	6.611059393344399E-5
134-135	0.0032228914542553944	6.590399832740197E-4	3.3055296966721993E-5	3.718720908756224E-5	0.1812545890048992	7.024250605428423E-5
136-137	0.0032270233663762348	6.673038075157003E-4	3.3055296966721993E-5	3.9253165147982365E-5	0.18209956503361102	7.437441817512448E-5
138-139	0.0032311552784970747	6.755676317573807E-4	3.3055296966721993E-5	4.545103332924274E-5	0.1828433092153623	7.437441817512448E-5
>>END_MODULE
